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1.
Three replicate paddocks, each of 0·235 ha, containing adjacent monocultures of perennial ryegrass or white clover [50:50 by ground area, 6 cm sward surface height (SSH) at start of experiment] were continuously stocked with three yearling and four mature non-lactating, non-pregnant Scottish halfbred ewes for 12 weeks. Herbage intake, grazing behaviour and dietary selection were measured on seven occasions. Clover SSH declined rapidly over the first 5 weeks then stabilized at 1·2–1·6 cm, whereas perennial ryegrass SSH rose slightly initially, then declined gradually. Animals initially included proportionately c . 0·6 white clover in their diet but, by the end of the experiment, this had fallen to 0·3. Total daily herbage intake declined over the 12 weeks from 1·8 kg dry matter (DM) day–1 at the start to 1·0 kg DM day–1. Total grazing time increased from 561 min day–1 to 649 min day–1 at the end of the experiment. The results suggest that, despite overall herbage depletion and a greater depletion of white clover than perennial ryegrass as a result of the initial partial preference for white clover, the animals traded-off a reduced total intake and an increased grazing time in an attempt to maintain their initial preferred dietary composition.  相似文献   

2.
A straw/concentrate mixture was offered to set-stocked dairy cows over a 24-week period. The cows were offered grazed herbage only (G), or grazed herbage with a straw/concentrate supplement offered either for 45 min after each milking (B), or overnight (P). The overnight treatment involved housing the cows between afternoon and morning milking. The straw/concentrate mixture contained 0·33 long barley straw, 0·28 barley, 0·12 soya bean meal, 0·25 molaferm and 0·02 minerals. During the first 8 weeks of the experiment an average of 2·25 kg of concentrate were fed, and from weeks 9–24, 2·0 kg of concentrate were fed.
The feeding of the straw/concentrate mixture led to a decrease in estimated herbage dry matter (DM) intake, particularly for treatment P. Estimated total DM intakes were increased throughout the experiment by offering the straw/concentrate mixture. However, total metabolizable energy (ME) intakes were only increased in mid-and late season.
Milk yield was higher in early season for treatment G; 28·1 kg d−1 compared to 26·8 kg d−1 and 25·5 kg d−1 for treatments B and P respectively. In late season the cows in treatment G had lower milk yields; 13·3 kg d−1 compared to 15·5 kg d−1 and 16·8 kg d−1 for treatments B and P respectively. Milk fat content was increased in early season in treatment P, and milk protein content tended to be reduced throughout the experiment for cows offered the straw/concentrate mixture overnight. Over the whole experiment there were no differences in yield of milk solids.  相似文献   

3.
Effects of timing and rate of N fertilizer application on concentrations of P, K, S, Ca, Mg, Na, Cl, Mn, Fe, Cu and Zn in herbage from perennial ryegrass/white clover pastures were studied at two sites in south-western Victoria, Australia. Nitrogen fertilizer (0, 15, 25, 30, 45 and 60 kg ha–1) was applied as urea in mid-April, early May, mid-May, early June and mid-June 1996 to pastures grazed by dairy cows. At Site 1, N fertilizer resulted in a linear increase in P, K, S, Mg and Cl concentrations in herbage and a linear decrease in Ca concentration. For all times of application, concentrations of P, K, Ca, Mg and Cl in herbage increased by 0·0048, 0·08, −0·010, 0·0013 and 0·053 g kg–1 dry matter (DM) per kg N applied respectively. For S concentration, maximum responses occurred in mid-May (0·012 g kg–1 DM per kg N applied). At Site 2, N fertilizer resulted in a linear increase in P, S and Na concentrations in herbage, a linear decrease in Ca concentration and a curvilinear increase in K and Cl concentration. The maximum responses for P, S and K concentrations in herbage occurred for the N application in mid-June and were 0·015, 0·008 and 0·47 g kg–1 DM per kg N applied respectively. For Cl concentration, the maximum response occurred for the N application in early June and was 0·225 g kg–1 DM per kg N applied. Overall, applications of N fertilizer up to 60 kg ha–1 did not alter herbage mineral concentration to levels that might affect pasture growth or animal health.  相似文献   

4.
An experiment was carried out in 1992 and 1993 to examine the effect of white clover content of perennial ryegrass/white clover swards on the performance of Limousin × Friesian heifers. Swards with low (L), medium (M) and high (H) white clover contents were established and managed by continuous variable stocking. A compressed sward height of 5·5 cm was maintained using a buffer fence to vary plot areas, with herbage surplus to grazing requirements cut, removed and yields measured. The mean white clover proportions for treatments L, M and H were 0·02, 0·19 and 0·18 in 1992 and 0·13, 0·16 and 0·31 in 1993 respectively. White clover contents of the swards reached a maximum in August and September, and differences between treatments diminished. There was no significant difference between treatments in the content of white clover in the swards in autumn 1993.
Liveweight gains of heifers increased asymptotically with increasing white clover content of the sward. Below a white clover herbage mass of 300 kg DM ha–1, there was little effect on liveweight gain, which was 0·70 kg day–1 over the grazing season. Between 400 and 450 kg DM ha–1 white clover, liveweight gains were 0·85–0·90 kg day–1. While clover content of the sward did not significantly affect utilized metabolizable energy output; the mean output over the grazing season in the two years from liveweight gain and herbage yield was 78 GJ ha–1. It is suggested that, using this grazing system, white clover reached an equilibrium with a mean herbage mass of about 400 kg DM ha–1 over the grazing season.  相似文献   

5.
Two experiments were carried out in Guadeloupe to estimate the organic matter intake (OMI) and digestibility (OMD) of a Dichanthium spp. sward, grazed by tethered Creole heifers [mean live weight (LW) 202 ± 2·0 kg], at three daily herbage allowances. Experiment 1 examined herbage allowances of 16, 25 and 31 kg of dry matter (DM) d–1 on a fertilized sward at 21 days of regrowth whereas, in experiment 2, lower allowances of 11, 15 and 19 kg DM d–1 were examined on the same sward, which was unfertilized and grazed at 14 days of regrowth. In each experiment, the herbage was grazed with three groups of two heifers in a 3 × 3 Latin square design. Sward characteristics were described before grazing. OMI was calculated from total faecal output, and OMD was predicted from the crude protein (CP) content of the faeces. The amount of herbage defoliated by the heifers was also estimated on tillers selected at random.
Organic matter intakes were on average 26 g and 19 g OM kg–1 LW, and OMD values were 0·740 and 0·665 for Experiments 1 and 2, respectively, and were not affected by allowance. In Experiment 1, the herbage quality was high [0·50 of leaf and 116 g CP kg–1 organic matter (OM)] for a tropical forage, whereas in Experiment 2, the quality of the herbage (0·27 of leaf and 73 g CP kg–1 OM) was lower. These differences were reflected in differences in intake and digestibility in the two experiments.
The experimental tropical Dichanthium spp. swards can have intake characteristics similar to those of a temperate sward.  相似文献   

6.
Over a 24-week period, three groups of dairy cows were continuously stocked at 8, 10 or 12 cows ha-1 between morning and afternoon milkings, and overnight were housed and offered grass silage ad libitum. Due to a prolonged drought, sward heights only averaged 4·1 cm.
The increase in daytime stocking rate led to a decline in herbage intake, and increases in silage intake. At the highest stocking rate (12 cows ha-1), the silage intake failed to compensate for the reduced herbage intake. Consequently the total dry matter and estimated metabolizable energy intakes were lower than for the 8 and 10 cows ha-1 treatments. Milk yields and milk composition were not significantly affected by treatment but the 12 cows ha-1 stocking rate gave the lowest milk and milk solids yields.
The utilized metabolizable energy (UME) on the grazed swards was greatest for the 10 cows ha-1 treatment. The sward cut to provide the silage had a UME level (GJ ha-1) 32% greater on average than the grazed swards during the same growth period. The total areas utilized for grazing and silage production for 8, 10 and 12 cows ha-1 were 0·240, 0·224 and 0·215 ha respectively. Fat and protein yields per unit area were greatest for the 10 cows ha-1 group.  相似文献   

7.
The effect of fasting on short-term cattle grazing behaviour   总被引：2，自引：0，他引：2  
The effects of fasting on feeding behaviour, intake rates, and diet quality were studied with steers grazing vegetative Lolium multiflorum swards. Fasting interrupted the normal alternation of grazing and rumination and led to longer grazing times (0·65 vs. 0·45 of observed time), shorter rumination times (0·15 vs. 0·30 of observed time), and higher total dry matter (DM) intakes (6200 g DM vs. 3750 g DM d−1)-Fasting did not affect prehended bite size which averaged 047 g DM bite−1. Declining bite size within a day was explicable by changes in herbage mass. Biting rate (38.9 bites min−1) and instantaneous intake rate (2675 g DM min−1) of fasted animals were considerably higher than non-fasting animals in the mornings, but neutral detergent fibre (NDF) concentration of the diet (0 742 of organic matter) was generally not depressed by fasting. Consequently, non-fasted animals appeared to be feeding suboptimally since their nutrient intake rates were considerably lower than that which they are capable of exhibiting. Mastication rates per unit NDF intake were considerably lower in the morning for fasted animals (0·78 chews g−1 NDF intake) which suggested that their intake rates may have led to larger ingested particle size. Therefore, on Lolium multifiorum swards, the steers attained a higher intake rate by masticating the forage less while maintaining diet quality.  相似文献   

8.
Changes in the crude protein (CP) concentration of white clover and perennial ryegrass herbage from a mixed sward were determined on six sampling dates from May to October in each of 2 years. The swards were grown without fertilizer N in an organic farming system and continuously grazed by dairy cows during the grazing season. The annual mean contents of white clover in the dry matter (DM) of the sward were 272·3 and 307·0 g kg−1 in Years 1 and 2. The mean CP concentrations of the white clover and perennial ryegrass herbage were 251·6 and 151·9 g kg−1 DM in Year 1 and 271·9 and 174·0 g kg−1 DM in Year 2 respectively. The CP concentration of the white clover increased significantly during the grazing season from 220·0 to 284·1 g kg−1 DM in Year 1 and from 269·0 to 315·5 g kg−1 DM in Year 2. In the perennial ryegrass herbage the CP concentration increased from 112·2 to 172·6 g kg−1 DM in Year 1 and from 142·7 to 239·5 g kg−1 DM in Year 2. The rate of increase during the season in the CP concentration of the perennial ryegrass herbage was similar to the rate of increase recorded in the white clover herbage.  相似文献   

9.
The effect of feeding either traditional concentrates containing starch or high quality fibrous concentrates on the performance of grazing dairy cows was examined in a trial in which cows were given concentrates with either 350 g starch and sugars (kg dry matter (DM))-1 (high-starch) or 100 g starch and sugars (kg DM)-1 (high-fibre). The swards used consisted predominantly of perennial ryegrass and were usually aftermaths following cutting. Each area was grazed for 3 or 4 d at each grazing and a two-machine sward-cutting technique was used for estimating herbage intake.
The effect of concentrate composition on the herbage intake of grazing cows at a high daily herbage allowance of 28 kg OM above 4 cm cutting height was investigated in 1983 and 1984. With 54 kg OM d-1 of high-starch concentrates the mean herbage intake was 11·5 kg OM d-1 per cow while cows fed 5.3 kg d-1 of high-fibre concentrates consumed on average 12–6 kg OM d-1. The mean substitution rate of herbage by concentrates was reduced from 0·45 kg herbage OM (kg concentrate OM)-1 with the high-starch concentrate to 0·21 with the high-fibre concentrates.
The effect of the treatments on milk production was studied in 1984. The cows consumed 5·5 kg OM d-1 as concentrates and grazed at a lower herbage allowance of 19 kg OM above 4 cm cutting height. With high-fibre concentrates milk production and 4% fat-corrected milk production were 13 and 1·8 kg d-1, respectively, higher than with the high-starch treatment. The daily live weight gain with the high-starch concentrates was 0·17 kg per cow more than with the high-fibre concentrates.  相似文献   

10.
In a two-year study, set stocked dairy cows were offered a mixture of brewers grains and sodium hydroxide-treated straw (1:1 on a dry matter basis)for 1 h daily. The experiments lasted for 20 and 19 weeks, mean supplementary (buffer) feed dry matter intakes were 3·0 and 3·4 kg d-1 and mean sward heights (rising plate meter) were 4·9 and 4·7 cm in 1988 and 1989 respectively. The control group received no supplementation. The buffer feed significantly increased milk yield (26·3 vs 24·6 kg d-1 and 28·6 vs 26·3kg d-1 in 1988 and 1989 respectively), increased liveweight gain (0·27 vs 0·13 kg d-1 and 0·27 vs 0·17 kg d-1) and increased effective stocking rate (5·17 vs 4·69 cows ha-1and 5·10 vs 4·53 cows ha-1). There were no significant effects on milk composition. The greatest responses in milk production were found in the late grazing season.  相似文献   

11.
Over a 24-week period during the 1986 summer, three groups of January- to March-calving dairy cows were either grazed conventionally (G) or grazed between morning and afternoon milkings and housed overnight and offered grass silage (Si) or a straw/concentrate mixture (St) ad libitum. The straw/concentrate mixture contained proportionately, 0-33 long barley straw, 0·28 ground barley, 0·12 soya bean meal, 0·25 molaferm and 0·22 minerals. The metabolizable energy (ME) and crude protein (CP) contents of the silage fed in weeks 1-8 and weeks 9-24 were 9-5 and 10−6 MJ kg MD−1 and 160 and 191 g kg DM−1 respectively. The straw mix had an ME content of 10−1 MJ kg DM−1 and CP content of 134 g kg DM−1. Partial storage feeding with silage or a straw/concentrate mixture led to a decrease in estimated herbage DM intake. The feeding of the straw/concentrate mixture increased total DM intake, but the estimated total ME intake was similar for treatments G and St. The intakes (kg DM d−1) for treatments G, Si and St were respectively, herbage 11·7,6·8,4·1; total l3·5,13·6,15·0; total ME intake (MJd−1) 163, 155, 163.
Animal performance was, for treatments G, Si and St respectively: milk yield (kg d−1) 19·2, 17·5, 19·1 (s.e.d. 0-87); milk fat content (g kg−1) 36·9, 37·6, 37.1 (s.e.d. 1.22); milk protein content (g kg−1) 35·3, 32·9, 33·4 (s.e.d. 0·76).  相似文献   

12.
The objective of this experiment was to use diurnal and temporal changes in herbage composition to create two pasture diets with contrasting ratios of water‐soluble carbohydrate (WSC) and crude protein (CP) and compare milk production and nitrogen‐use efficiency (NUE) of dairy cows. A grazing experiment using thirty‐six mid‐lactation Friesian x Jersey cows was conducted in late spring in Canterbury, New Zealand. Cows were offered mixed perennial ryegrass and white clover pastures either in the morning after a short 19‐day regrowth interval (SR AM) or in the afternoon after a long 35‐day regrowth interval (LR PM). Pasture treatments resulted in lower pasture mass and greater herbage CP concentration (187 vs. 171 g kg?1 DM) in the SR AM compared with the LR PM but did not affect WSC (169 g kg?1 DM) or the ratio of WSC/CP (1·0 g g?1). Cows had similar apparent DM (17·5 kg DM cow?1 d?1) and N (501 g N cow?1 d?1) intake for both treatments. Compared with SR AM cows, LR PM cows had lower milk (18·5 vs. 21·2 kg cow?1 d?1), milk protein (0·69 vs. 0·81 kg cow?1 d?1) and milk solids (1·72 and 1·89 kg cow?1 d?1) yield. Urinary N concentration was increased in SR AM, but estimated N excretion and NUE for milk were similar for both treatments. Further studies are required to determine the effect of feeding times on diurnal variation in urine volume and N concentration under grazing to predict urination events with highest leaching risk.  相似文献   

13.
The development of efficient forage management and utilization is facilitated by a knowledge of the changes in nutritive value and patterns of dry matter (DM) distribution within plants. The objectives of this experiment were to determine the patterns of DM accumulation and the change in nutritive value during maturation of crimson clover ( Trifolium incarnatum L.), a cool-season annual forage legume adapted to mild winter areas and acid soils. Crimson clover was established on a Dystric Eurocrept soil with pH 5·4 in Galicia, north-western Spain, during three consecutive years (1990–92). Shoot samples were taken at approximately biweekly intervals in the spring and summer after autumn sowing, and were separated into stems, leaves and seed pods. DM accumulation, crude protein (CP) and acid-detergent fibre (ADF) concentrations and in vitro organic matter digestibility (IVOMD) were determined for each plant part at each stage. Average annual DM production at initial flowering was 4·2 t ha–1, whereas the CP and ADF concentrations and IVOMD values were 134 g kg–1 DM, 302 g kg–1 DM and 0·720 respectively. The nutritive value declined with age, coinciding with a reduction in leaf:stem ratio (from 0·53 at the vegetative stage to 0·13 at the ripe seed stage). This decline was more rapid than that reported for other legumes, possibly because of the low leaf:stem ratio of crimson clover. However, as it is used in the Atlantic coastal areas of north-western Spain, where it is harvested around initial flowering, it can provide forage of good quality.  相似文献   

14.
Three groups of cows were continuously stocked on pasture and offered supplementary concentrates according to the herbage height measured weekly with a grass disc. Concentrates were offered when the mean herbage height fell below 9 cm for treatment A, 7 cm for treatment B and 5 cm for treatment C at a rate of 1 kg d-1 for each 0·2-cm decline below these threshold levels. If the herbage height declined by more than 1·2 cm (8 kg concentrates d-1) hay was offered in addition ad libitum. The stocking rates for all three treatments were 5·2, 3·2 and 3·2 cows ha-1 for three successive 8-week periods. For treatments A, B and C the mean concentrate DM intakes were 3·2, 1·2 and 1·2 kg d-1 (treatment A also received 0·2 kg hay DM d-1), and the mean daily milk yields were 17·2, 16·2 and 15·2 kg respectively. The increasing level of supplementary feeding from treatment C to A also resulted in an increase in liveweight and body condition score change, and a reduction in milk fat.  相似文献   

15.
Management of beef cows grazing extensively grazed semi-natural pastures in temperate regions in late autumn can require supplements to be offered. The effects of supplementation with soya bean meal on the diet selected by Charolais cows and on their subsequent performance were examined for an 8-week period in late autumn in 2 years. Three groups of eight cows were compared: non-supplemented dry cows (D), non-supplemented (L) and supplemented (LS) lactating cows. The amount of soya bean meal supplement offered per cow was 250 g d−1 in year 1 and 800 g d−1 in year 2. Dietary choices were measured by direct observations and herbage intake was estimated in year 2. Supplementation affected neither diet selection (L: 0·42 vs. LS: 0·43 for the proportion of bites on green patches in year 1; 0·24 vs. 0·22 in year 2) nor daily organic matter intake of herbage (L: 15·6 vs. LS: 15·9 kg d−1), which may have resulted from an adequate crude protein concentration of herbage. The higher total dry matter intake by cows offered the supplement reduced losses in live weight (L: −1212 vs. LS: −828 g d−1; P  < 0·01) rather than increased milk production (L: 5·1 vs. LS: 5·0 kg d−1). This may be linked to the low milk yield potential of the Charolais cows. The use of lactating cows rather than dry cows for pasture management in late autumn would increase the utilization of herbage but a reduction in liveweight losses of cows by supplementation is unlikely to be economic.  相似文献   

16.
The sulphur status of four soils and 122 forage plants from the Inner Mongolia steppe was determined. The organic sulphur concentration ranged from 17 μg g–1 in the 0·2–0·4 m soil layer to 397 μg g–1 in the topsoil. The mean sulphate-S concentrations were <10 μg g–1; greater concentrations were found only in the chernozem soil. Biomass-S accounted for 0·018–0·028 of total S in four soils. Three-quarters of plant samples examined had <1·6 g kg–1 S, and >0·80 of them had an N:S ratio >14:1. More than 0·80 of the plants were deficient in S. There was a close relationship between plant-available soil sulphur concentrations and total plant sulphur concentrations. It was concluded that sulphur deficiency is widespread in the Inner Mongolia steppe and that sulphur fertilizer requirements should be evaluated.  相似文献   

17.
The content of ergot alkaloids (ergovaline and chanoclavine), and their production in October 1996 and during the period May–September 1997, were investigated in seventeen ecotypes of perennial ryegrass ( Lolium perenne L.) and in nineteen ecotypes of meadow fescue ( Festuca pratensis Huds.), naturally infected with Neotyphodium spp. The ecotypes were collected in the north-eastern part of the Czech Republic. In 1996 the content of ergovaline in the ecotypes of perennial ryegrass ranged from 0·00 to 2·73 μg g–1 dry matter (DM) (one cut), and in 1997 from 0·00 to 4·65 μg g–1 DM (five cuts). In meadow fescue the content of ergovaline varied from 0·00 to 0·61 μg g–1 DM (one cut) in 1996, and in 1997 from 0·00 to 2·31 μg g–1 DM (five cuts). The content of chanoclavine (investigated in 1997 in four cuts only) in perennial ryegrass ranged between 0·00 and 3·39 μg g–1 DM, and in meadow fescue between 0·00 and 2·26 μg g–1 DM. Most ecotypes of L. perenne reacted to the high temperature and heavy rainfall in June and July of 1997 with an enhanced production of ergovaline, whereas the content of chanoclavine was not changed. Such reaction to stress conditions was not observed in the ecotypes of F. pratensis. Large differences in the production of both ergot alkaloids between different ecotypes of both plant species were observed.  相似文献   

18.
The effects of sowing date and autumn management of sainfoin ( Onobrychis viciifolia Scop.) were investigated over 3 years in the UK. Replicated plots were sown between April and September in 2003 and 2004. Autumn management treatments were early and late cutting carried out in the establishment year and in subsequent years. Dry matter (DM) yields were measured over 3 years. One harvest was taken from April to July sowings in the establishment year and three harvests in each of the following years. DM yields from sowings in April and May were 2·8 and 3·3 t DM ha−1, respectively, in the establishment year, which were higher ( P  <   0·001) than from sowings in June and July. Sowings from April to July yielded 10·9–12·5 t DM ha−1 in the first full-harvest year, and 9·5–11·5 t DM ha−1 in the following year. Sowings in August and September only gave 5 t DM ha−1 year−1. Early-autumn cutting of an established sward reduced yields of sainfoin at the second harvest in the first and second full-harvest years. Sowing in May had the lowest proportion of weed species (0·06) in the establishment year, and sowing in July had the highest (0·53). Crude protein concentration increased as the seasons progressed from 149·8 to 230·1 g kg−1 DM.  相似文献   

19.
An experiment was conducted to examine the effect of concentrate supplementation on the performance of suckling lambs while grazing at variable levels of herbage allowance. Twenty-four ewes suckling twin lambs were allocated 55 d after lambing between four grazing treatments (two levels of herbage allowance × two levels of supplementation) in a 2×2 factorial design. The sward consisted of Tall Fescue and was grazed rotationally.
Daily herbage organic matter (OM) allowances were 57.0 (H) and 38.5 (L) g OM kg LW−1 above 1·5 cm cutting height. Lambs were either supplemented (S) ad libitum with high-moisture whole maize grain or unsupplemented (U).
Supplement intake during the 60-d grazing period was 16 kg DM and 17.5 kg DM respectively for LS and HS lambs. The effect of supplementation on lamb growth rate differed significantly between allowances: at the low allowance level, supplementation increased growth rate (287 g d−1 vs 226 g d−1), whereas it had no effect at the high level (277 g d−1 vs 276 g d−1). Growth rate of unsupplemented lambs was significantly reduced at the low level of allowance compared to the high level (226 g d−1 vs 276 g d−1). The effect of sward height on time spent grazing by unsupplemented lambs is described during the defoliation of a plot.  相似文献   

20.
The variation in force required to break the leaves and pseudostems of perennial ryegrass ( Lolium perenne ) was measured on twelve New Zealand commercial and pre-release cultivars using a punch and die apparatus which enabled up to 100 simultaneous breaks per sample. Perennial ryegrass cultivars were sampled from 2-year-old pure swards in two studies carried out in spring and autumn. In the first study, the forces required to break old and young leaves, and pseudostems, of six cultivars ranging in heading date and ploidy were compared at a designated morphological stage during spring growth. Force was measured as Newtons (N) mg−1 dry matter (DM) and the difference between the force required to break leaves and pseudostems was significant ( P  < 0·01). The youngest emerged leaf required a greater force by 0·13 than the oldest green leaf (5·60 and 4·90 N mg−1 DM for young and old leaf respectively). The force required to break leaves (average of young and old leaves) of 5·25 N mg−1 DM was 0·12 greater than the force required to break pseudostems (4·59 N mg−1 DM). This difference was consistent across most cultivars. In the second study, the force required to break leaves of ten cultivars was tested daily over three autumn periods when all cultivars were sampled at a regrowth age of 2 weeks. The cultivar Nevis was used in each period whilst the other cultivars were used in one period. When averaged across periods, cultivars could be separated into three groups of high, medium and low forces required to break leaves. The difference between the means of the high and low groups was 0·33 with a range from 3·29 to 4·91 N mg−1 DM.  相似文献   

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