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NaCl胁迫对莴笋种子萌发及幼苗根系生理生化指标的影响 总被引：4，自引：0，他引：4
研究了NaCl胁迫对2个莴苣品种种子萌发及幼苗生长的影响.研究结果表明,随着NaCl胁迫浓度的增大,对2个参试品种种子萌发及幼苗生长的抑制作用明显增强.同时采用营养液水培法,研究了盐胁迫对其幼苗根系活力、相对电导率、丙二醛(MDA)含量、超氧化物歧化酶(SOD)和过氧化物酶(POD)活性的影响.研究结果表明,2个品种根系的相对电导率和MDA含量均随着NaCl浓度的增加呈现出增大的趋势,而2个品种的根系抗氧化酶(SOD和POD)活性和根系活力则随着NaCl浓度的增加而呈现出降低的趋势,并且每个盐浓度处理下相对电导率和MDA含量较对照的增强程度、SOD和POD活性和根系活力较对照的下降程度"攀研""绵兴". 相似文献
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以黄瓜‘中农18’为试材,在苗期采用黑籽南瓜作为砧木采用插接法进行嫁接换根,对嫁接苗与自根苗进行干旱胁迫,测定株高、茎粗、干物质质量、叶绿素含量、过氧化物酶(POD)活性、超氧化物歧化酶(SOD)活性、过氧化氢酶(CAT)活性、丙二醛(MDA)含量、根系活力、脱落酸(ABA)含量、玉米素(ZR)含量,研究干旱胁迫对嫁接黄瓜幼苗生长发育的影响,以期为黄瓜逆境生理的研究提供参考依据。结果表明：在干旱胁迫下嫁接苗、自根苗的生长发育均受影响,表现为株高、茎粗、干物质质量的积累减缓,与自根苗相比较,嫁接苗的株高、茎粗、干物质质量较高,处理9 d后分别达17.54 cm、6.24 mm、1.2 g。进一步分析生理指标,可以发现干旱胁迫对黄瓜嫁接苗、自根苗均有一定程度的抑制,表现为叶绿素含量、POD活性、SOD活性、CAT活性、内源激素含量在前期表现为升高,后期开始下降,MDA含量逐渐升高,嫁接苗的根系活力表现为先升高后下降,自根苗的根系活力逐渐下降。除MDA含量外嫁接苗的生理指标均高于自根苗,嫁接苗的叶绿素含量、POD活性、SOD活性、CAT活性、MDA含量、根系活力、内源激素含量最高峰达2.58... 相似文献
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以"津研4号"黄瓜为试材,研究了6-BA浸种对淹涝胁迫下黄瓜幼苗根系活力、根系电导率、保护酶活性、乙醇脱氢酶(ADH)活性的影响。结果表明:淹涝使黄瓜幼苗根系活力显著下降,根电导率显著升高,6-BA浸种可延缓淹涝胁迫下黄瓜幼苗根系中的根系活力的降低和根系电导率的升高;淹涝使黄瓜根系的超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、乙醇脱氢酶(ADH)活性均呈先升后降的趋势,6-BA浸种处理可以使淹涝胁迫下的黄瓜在较长时间内维持较高的保护酶和ADH活性,从而提高黄瓜对淹涝胁迫的抵抗能力。 相似文献
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摘要：为探索缓解樱桃萝卜盐胁迫效应的化学调控措施,研究了NaCl（0、50、100、150 mmol/L）胁迫对樱桃萝卜种子萌发和幼苗生长的影响以及赤霉素（0、10、20、30、40 mg/L）对100 mmol/L NaCl胁迫的调控作用。结果表明：NaCl胁迫显著抑制了樱桃萝卜种子的萌发和幼苗生长,且NaCl浓度越大,抑制作用越强。在100 mmol/L NaCl处理下,种子发芽率、发芽势、发芽指数、活力指数、株高、单株鲜质量、根长、根表面积、根投影面积、根体积、根平均直径、根尖数、根分枝数、根系活力、叶绿素a含量、叶绿素b含量分别较对照显著降低4.33百分点、5.33百分点、30.05%、26.01%、20.88%、19.52%、50.08%、70.27%、70.83%、100.00%、23.08%、15.69%、22.73%、10.67%、12.86%、23.81%,过氧化氢
酶（CAT）活性较对照显著提高13.51%。在100 mmol/L NaCl胁迫下,采用30 mg/L赤霉素进行调控处理,樱桃萝卜的种子发芽率、发芽势、发芽指数、活力指数、根长、根表面积、根投影面积、根平均直径、根尖数、根分枝数、株高、叶绿素a含量、叶绿素b含量分别较对照显著提高4.78百分点、12.96百分点、23.09%、42.03%、108.44%、135.29%、140.00%、79.17%、15.45%、114.29%、15.38%、41.67%、70.00%,CAT活性则较对照显著下降33.33%,根系生长发育得到显著改善,株高和叶绿素含量均显著增加;但是当赤霉素浓度为40 mg/L时,效果开始减弱。说明低浓度赤霉素可以缓解盐胁迫对樱桃萝卜造成的抑制作用,但是浓度过高可能会造成新的胁迫,30 mg/L赤霉素是缓解樱桃萝卜盐胁迫的合适浓度。 相似文献
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The effects of combinations of salinity (no salt, 2000 p.p.m. or 4000 p.p.m. of CaCl2 and NaCl, 1:1) and water table (30 cm, 60 cm or 90 cm from the soil surface), on the vegetative growth and tolerance of ‘Golden Japanese’ plum and ‘Mit Ghamre’ and ‘Balady’ peaches were studied. The plants were grown in lysimeters. The growth of the trunk, total shoot length, the increase in shoot length per cm and the fresh weight of top, root and total plant were reduced with increasing salinity of the irrigation water. The effect was accentuated when the plants were maintained at high water table level. The salinity treatments resulted in the death of 43%, 73% and 76% of the plants in the plum, and the ‘Mit Ghamre’ and ‘Balady’ peaches, respectively, indicating that the plum is more tolerant to salinity than the peach. The plants of the salinity treatments showed various symptoms of salt injury, such as leaf burn, defoliation, shoot die-back and finally death. In the peaches, salt injury started to occur in the first growing-season, whereas the salt injury appeared in the plum in the second growing-season. The symptoms were more pronounced in the 4000 p.p.m. treatment than in the 2000 p.p.m. treatment and were more pronounced at the high water table level. The salinity level was the predominant factor and the effect of the water table on the vegetative growth diminished with increase in the salinity level of the irrigation water. 相似文献
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AIM：To establish a fast, accurate and economical technique for culturing mouse pulmonary arteriolar smooth muscle cells (PASMCs), and to explore the effects of hypoxia on the proliferation and apoptosis of the PASMCs. METHODS：In sterile condition, the pulmonary artery was isolated from the male BALB/c mice by digesting with collagenase I, and the cells were cultured in fetal bovine serum-coated flask. Centrifugal procedure was not used during the cell passage. The cell morphology was observed under an inverted phase-contrast microscope. α-Smooth muscle actin was identified by immunocytochemistry and immunofluorescence. The effects of hypoxia on the proliferation and apoptosis of the PASMCs were detected by CCK-8 assay and TUNEL assay. RESULTS：PASMCs were identified by the methods of immunocytochemistry, immunofluorescence staining and observation of morphology. Unlike the rat PASMCs with typical subcultured peak-vally pattern, the mouse PASMCs showed a lot different without a peak-vally pattern. The cells could be subcultured after 5 d to 7 d and there was 3 to 5 generations depending on the activity of the cells. CCK-8 assay demonstrated that the A values of PASMCs exposed to hypoxia increased after 24 h (P＜0.05) as compared with normoxia. TUNEL result showed that the apoptotic index of the PASMCs in hypoxia decreased after 24 h (P<0.05). CONCLUSION：This technique for obtaining cultured mouse PASMCs is simple, fast, accurate and economical. The digestion time is easy to control. Hypoxia promotes the proliferation and inhibits the apoptosis of PASMCs. 相似文献
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小檗碱和壳聚糖抗蔬菜病原真菌活性测定及复合膜制备 总被引：2，自引：0，他引：2
研究了小檗碱和壳聚糖对几种常见蔬菜病原真菌的抑制活性,以及以小檗碱和壳聚糖为主料制备复合膜的方法,并测定了该膜的药物释放效果。试验表明:低浓度(0.234 mg/mL)小檗碱即可抑制辣椒炭疽病菌(Vermicularia capsici)等5种蔬菜病原真菌的生长。20 mg/mL浓度壳聚糖对番茄灰霉病菌(Botrytis cinerea)的抑制率高达65%,而对其余4种果蔬病原真菌也有一定的抑制作用。为了集成这2种天然化合物的优点,制备了小檗碱-壳聚糖复合膜,该膜具有缓释功能,在模拟外部环境(磷酸缓冲液,pH 6.8)条件下,20 d小檗碱累计释放率接近25%,提示其在果蔬贮藏抗菌中的应用价值。 相似文献
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Fresh onion seeds desiccated to 6.0% seed moisture content (SMC) were stored in various packaging materials under different storage conditions. Seeds packed in aluminum-laminated pouches beside those stored with silica gel at 25 °C maintained satisfactory germinability and vigour after 12 months. Desiccated seeds stored in moisture impervious containers produced more vigourous seedlings. Germination potential of onion seeds increased with reduced SMC besides storage in moisture impervious packets along with desiccants as physiological and biochemical attributes are regulated. Seed viability and vigour decreased with accelerated ageing due to increased lipid peroxidation, decreased activities of several free radical and peroxide scavenging enzymes. Electrical conductance of seed leachates also increased with ageing. Thus, adoption of appropriate storage temperature and moisture control techniques would significantly affect onion seed quality, which was due to minimum accumulation of free peroxide radicals and enhanced activity of free radical scavenging enzymes. 相似文献
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以大荔县冬枣种植区采集的发病枣果为试材,采用常规组织分离法进行病原菌的分离纯化,通过柯赫氏证病法则、形态学观察及分子生物学分析,研究鉴定了冬枣黑斑病和炭疽病的病原菌种类,并采用田间调查法对2种病害为害特征进行比较分析,以期为田间有效区分冬枣黑斑病和炭疽病为害症状,并快速判断其病原类型,及进一步深入开展精准防控提供参考和指导.结果表明:陕西大荔冬枣黑斑病的病原菌为细极链格孢(Alternaria tenuissima),炭疽病的病原菌为胶孢炭疽菌(Colletotrichum gloeosporioides).2种病害均主要为害枣果实,在枣果果腰或果肩上形成黑褐色病斑,可能同时存在且症状相近极易混淆,但在病斑表面、皮下及久置表型3个方面存在明显区别. 相似文献
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钾肥在小白菜和萝卜上的施用效果 总被引：6，自引：0，他引：6
采用盆栽试验方法研究了施用钾肥对小白菜、萝卜生长发育、产量、品质及钾素含量的影响。结果表明 ,施用钾肥对小白菜和水萝卜的株高、叶片数无显著的影响 ,但可提高水萝卜块根的产量 ,提高幅度为 31.0 4 %～ 97.93%。施用钾肥可提高小白菜和萝卜的蛋白质和VC含量 ,降低硝酸盐含量 ,增加植株的钾素含量 相似文献