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1.
Root drench application of Trichoderma atroviride isolates R32, R33, R40 and R84 promoted the growth of potted radiata pine seedlings. After 6 weeks, seedlings treated with R33 and R84 had thicker stems and greater stem and root biomass (p < 0.05) than untreated controls. Treatment with R32 increased seedling root biomass whilst R40 increased stem diameter. None of the isolates affected seedling height. One isolate, R33, induced systemic resistance to stem inoculation with Diplodia pinea and reduced dieback incidence by 20% compared with untreated controls. To our knowledge, this is the first report of systemic induced resistance by Trichoderma in a pine species. Furthermore, seedlings that were treated with R33 (root drench) plus foliar application of methyl jasmonate (MeJA) expressed elevated peroxidase activity in their stems 2 weeks later, compared with seedlings treated only with MeJA. Because R33 itself did not affect peroxidase activity, this may be indicative of treatment synergy or defence potentiation by R33. Curiously, R33 + MeJA induced terpenoids but suppressed phenylalanine ammonia‐lyase activity suggesting possible trade‐offs between phenolic and terpenoid defence pathways in the treated seedlings.  相似文献   

2.
The effects of fertilization and irrigation, singly and in combination, on growth and dry-matter allocation in seedlings of Pinus taeda and P. elliottii were investigated under field conditions. Trickle irrigation was regulated to maintain soil water potential above −10 kPa and 111 g of fertilizer (N:P:K 10:10:10 plus micronutrients) were banded around each seedling on three occasions. Treatments were initiated at the beginning of the second growing-season in the field, the same year sampling was conducted. Sampling consisted of complete excavation of 15 seedlings per treatment per species beginning in early April and continued every 6 weeks through mid-November, 1986. Each seedling was measured for height, root-collar diameter, foliage, stem and root dry-matter, and total needle surface-area. Allometric growth-analysis was used to determine dry-matter partitioning among the various tree components.

The cultural treatments affected the two species differently. Loblolly pine responded to treatments by shifting dry-matter allocation from roots to shoots, with the greatest increase observed in the fertilization treatment. Slash pine showed a similar response to irrigation and to irrigation plus fertilization, but increased allocation to roots under the fertilization treatment. Allocation to stems was greater than to foliage in both species and treatments except the control loblolly pine.  相似文献   


3.
Maintaining proper seedling nitrogen status is important for outplanting success. Fall fertilization of evergreen conifer seedlings is a well-known technique for averting nitrogen (N) dilution caused by continued seedling growth during hardening. For deciduous seedlings, this technique is much less understood, and regardless of foliage type, the interaction of N status prior to fall fertilization and the rate of fall fertilization have yet to be fully explored. Therefore, we fertilized Quercus variabilis container seedlings with either 25, 100, or 150 mg total N seedling?1, applied exponentially, during a 23-week pre-hardening regime, followed by either 0, 12, or 24 mg total N seedling?1 applied during hardening (i.e., fall fertilization) in equal aliquots for 4 weeks. For seedlings without supplemental N during hardening, N concentration in stems and roots increased significantly despite substantial growth. The absence of N dilution was attributed to N translocation from foliage to these tissues, which was independent of pre-hardening N status. Overall, 32 % of foliar N was translocated and accounted for 75 % of the total N increase in stems and roots. Final stem N status was a function of pre-hardening fertilization, whereas root N concentration was affected by the interaction of pre-hardening and fall fertilization. Roots appear to be the main site of N storage, and root N content was significantly affected by pre-hardening and fall fertilization, but not their interaction. A combination of pre-hardening and fall fertilizer at a rate of 100 and 24 mg total N seedling?1, respectively, yielded seedlings with the largest root systems.  相似文献   

4.
Two-year-old Fraxinus mandshurica Rupr. var. japonica Maxim. seedlings were flooded to 8 cm above soil level for 70 days. The flooding treatment altered the growth, morphology, stem anatomy and ethylene production of the seedlings. Although flooding did not affect height growth, it stimulated diameter growth of the submerged stems by increasing both the number and size of wood fibers produced; however, the thickness of the cell walls of the wood fibers was reduced by flooding. In response to the flooding treatment, the seedlings formed abundant hyperhydric tissues, originating from the vicinity of lenticels on the surface of the flooded stems, and adventitious roots, which grew through the hyperhydric tissues. Aerenchyma tissues were observed in the bark of the adventitious roots. The flooding treatment did not affect dry weight increment of leaves and stems, but it reduced the total dry weight increment of the root system even though it promoted adventitious root formation. Flooding also enhanced ethylene production in the submerged portions of stems. The potential roles of flood-induced ethylene in cambial growth and adventitious root formation in flooded plants are discussed.  相似文献   

5.
Seasonal patterns of carbohydrate concentration in coarse and fine roots, stem or bole, and foliage of ponderosa pine (Pinus ponderosa Laws) were described across five tree-age classes from seedlings to mature trees at an atmospherically clean site. Relative to all other tree-age classes, seedlings exhibited greater tissue carbohydrate concentration in stems and foliage, and greater shifts in the time at which maximum and minimum carbohydrate concentration occurred. To determine the effect of environmental stressors on tissue carbohydrate concentration, two tree-age classes (40-year-old and mature) were compared at three sites along a well-established, long-term O3 and N deposition gradient in the San Bernardino Mountains, California. Maximum carbohydrate concentration of 1-year-old needles declined with increasing pollution exposure in both tree-age classes. Maximum fine root monosaccharide concentration was depressed for both 40-year-old and mature trees at the most polluted site. Maximum coarse and fine root starch concentrations were significantly depressed at the most polluted site in mature trees. Maximum bole carbohydrate concentration of 40-year-old trees was greater for the two most polluted sites relative to the cleanest site: the bole appeared to be a storage organ at sites where high O3 and high N deposition decreased root biomass.  相似文献   

6.
Sucrose synthase (SS) was the dominant enzyme of sucrose metabolism in both stem and root vascular cambial zone tissues of nursery-grown loblolly pine (Pinus taeda L.) seedlings. Acid invertase (AI) and neutral invertase (NT) activities were generally less than 10% of the SS activity in both tissues. In both cambial tissues, seasonal patterns of enzyme activity were observed for SS but not for AI or NI. The seasonal patterns of SS activity in stem and root cambia paralleled the periodic growth of stems and roots. Stems had high SS activity and growth during summer and early fall. Roots had substantial SS activity and growth during summer and fall, but SS activity and growth were even higher in winter. When seedlings were transplanted, about eight months elapsed before stem and root cambia resumed rates of growth and sucrose metabolism similar to those in control nontransplanted seedlings. Two months after transplanting, root SS was at its lowest, whereas AI activity in transplants was 50% higher than in control nontransplanted seedlings. In stems, SS activity decreased in response to transplanting, whereas AI and NI activities did not change appreciably. In loblolly pine tissues, SS was specific for uridylates, whereas the nucleotide triphosphate-dependent phosphofructokinase (NTP-PFK) had similar activity with either UTP or ATP. Except in winter, the NTP-PFK was less active than the pyrophosphate-dependent phosphofructokinase (PPi-PFK) during all seasons. The PPi-dependent PFK activity in nontransplanted seedlings followed similar seasonal and spatial patterns to those of SS activity. In actively growing tissues, such as stem cambial tissues in summer and root cambial tissues in winter, the measured total PFK to SS ratio ranged between 1.5/1 and 3/1. In contrast, in less actively growing tissues or transplanted seedlings, a greater decrease occurred in SS than in PFK activity, hence the ratio rose to as high as 12/1. It was concluded that: (1) SS was the dominant enzyme for sucrose metabolism in root and stem cambial tissues of loblolly pine seedlings; (2) both SS and PPi-PFK in the cambial tissues can be used as biochemical indicators of growth sink strength in stems and roots; and (3) both enzymes can be used as indicators of seedling stress caused by events such as transplanting and winter freezing.  相似文献   

7.
Sperry JS  Ikeda T 《Tree physiology》1997,17(4):275-280
Roots of hardwoods have been shown to be more vulnerable to xylem cavitation than stems. This study examined whether this pattern is also observed in a conifer species. Vulnerability to cavitation was determined from the pressure required to inject air into the vascular system of hydrated roots and stems, and reduce hydraulic conductance of the xylem. According to the air-seeding hypothesis for the cavitation mechanism, these air pressures predict the negative xylem pressure causing cavitation in dehydrating stems. This was evaluated for stems of Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) and white fir (Abies concolor (Gord. & Glend.) Lindl.). The air-injection method was applied to roots and stems of different sizes and positions in Douglas-fir trees. Roots, especially smaller roots with a xylem diameter < 5 mm, were more vulnerable to cavitation than stems. Mean cavitation pressure for smaller roots was -2.09 +/- 0.42 versus -3.80 +/- 0.19 MPa for larger roots (> 8 mm diameter). Within the shoot system, smaller stems (< 5 mm diameter) were most vulnerable to cavitation, having a mean cavitation pressure of -4.23 +/- 0.565 versus -5.27 +/- 0.513 MPa for large stems (> 8 mm diameter). There was no correlation between tracheid diameter and mean cavitation pressure within root or stem systems, despite larger tracheid diameters in roots (23.3 +/- 3.9 micro m) than in stems (9.2 +/- 1.6 micro m). Smaller safety margins from cavitation in roots may be beneficial in limiting water use during mild drought, and in protecting the stem from low xylem pressures during extreme drought.  相似文献   

8.
Flooding of soil for 55 days altered the rate of growth and stem anatomy of 9‐month‐old Cryptomeria japonica seedlings. Although flooding did not affect height growth it reduced the rate of dry weight increment of seedlings while increasing stem diameter. The reduction in dry weight increment of seedlings resulted largely from decay of roots and, to a lesser extent, from inhibition of growth of roots and needles. The increased diameter growth of flooded seedlings resulted largely from an increase in bark thickness associated with increased phloem production and greater amount of intercellular space. Flooding reduced xylem increment in submerged stems but increased it above the water level because of larger tracheids rather than more tracheids per radial file. Flooding also increased lumen diameters of tracheids, decreased tracheid wall thickness (as a proportion of tracheid diameter), and stimulated formation of axial parenchyma cells in the xylem. Cryptomeria japonica seedlings adapted to flooding by forming adventitious roots, primarily on the original root system and submerged portion of the stem. Such new roots originated in the xylem ray parenchyma. Flooding stimulated ACC synthesis in roots and ethylene production in stems. The role of ethylene in alteration of stem anatomy is discussed.  相似文献   

9.
The objectives of this study were to assess the range of genotypic variation in the vulnerability of the shoot and root xylem of Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) seedlings to water-stress-induced cavitation, and to assess the trade-off between vulnerability to cavitation and conductivity per unit of stem cross-sectional area (k(s)), both within a species and within an individual tree. Douglas-fir occupies a broad range of environments and exhibits considerable genetic variation for growth, morphology, and drought hardiness. We chose two populations from each of two varieties (the coastal var. menziesii and the interior var. glauca) to represent environmental extremes of the species. Vulnerability curves were constructed for shoots and roots by plotting the percentage loss in conductivity versus water potential. Vulnerability in shoot and root xylem varied genetically with source climate. Stem xylem differed in vulnerability to cavitation between populations; the most mesic population, coastal wet (CW), was the most susceptible of the four populations. In the roots, the most vulnerable population was again CW; the interior wet (IW) population was moderately susceptible compared with the two dry populations, coastal dry (CD) and interior dry (ID). Root xylem was more susceptible to cavitation than stem xylem and had significantly greater k(s). The trade-off between vulnerability to cavitation and k(s), however, was not evident across populations. The most vulnerable population (CW) had a shoot k(s) of 0.534 +/- 0.067 &mgr;mol m(-2) s(-1) MPa(-1), compared with 0.734 +/- 0.067 &mgr;mol m(-2) s(-1) MPa(-1) for the less vulnerable CD stems. In the roots, IW was more vulnerable than ID, but had the same k(s).  相似文献   

10.
红豆树根瘤的形成时间和根瘤的数量与圃地土壤的质量有关,在土壤水肥条件好的环境中,1 a生苗木中的根瘤少见,且根瘤个体极小;在土壤较贫瘠的圃地中,红豆树根瘤十分发达,数量多且形成时间早。红豆树根瘤能够促进苗木生长,有根瘤生长比无根瘤生长的1年生红豆树植株中,根系的干物质增长率46%、根系鲜重的增长率41.9%、茎干物质增长率40.3%、植株总干物质的增长率35.0%、植株总鲜重增长率22.4%、植株叶的干物质增长率14.8%。截根处理有助于根瘤菌感染苗木植株,苗木生长更健壮:截根处理的苗木径高比为4.1%,未截根处理的为3.4%;截根处理促进根系的发育,截根处理的侧根总长度47cm/株、未截根处理的侧根总长度为26cm/株、侧根增加率为80.8%,截根处理的须根总长度228cm/株、未截根处理的侧根总长度为102cm/株、须根增加比率123.5%;截根处理促进苗木根幅的扩大,比未截根处理的根幅增大27.3%。  相似文献   

11.

A plant's induced defense system can be triggered by the application of the plant hormone methyl jasmonate (MeJA), and recent research suggest that MeJA treatment may become a tool for protection of conifer seedlings against insect herbivory (e.g. by the pine weevil Hylobius abietis). A side-effect of MeJA application is temporarily reduced height growth. This has generally been considered as negative, but in forest tree nurseries this could instead be beneficial since it is commonly desired to stop the growth of nursery seedlings in late summer. Artificially longer dark periods (long nights/short days) are widely used in high-latitude nurseries to terminate height growth and induce freezing tolerance. However, long night treatment requires specialized nursery equipment and are labor intensive. Therefore alternatives are sought after. We compared long-night and MeJA treatments by following the growth of Norway spruce (Picea abies) seedlings throughout one season. The regulatory effect of MeJA on height growth was similar if not even better than that of long nights, i.e. it was terminated faster. However, MeJA treatment also reduced root growth and delayed the development of freezing tolerance. MeJA may therefore not replace long-night treatments, but it could facilitate a more flexible application of long nights by gaining a longer time interval during which this treatment can be used without risking the seedlings growing too large.

  相似文献   

12.
茉莉酸甲酯(MeJA)是茉莉酸的一种衍生物,外源使用可诱导植物产生防御反应,包括化学和物理防御,提高植物的抗性。为了搞清其是否对蒙古扁桃产生诱导防御反应,研究了不同浓度的茉莉酸甲酯对蒙古扁桃木质素、晶细胞及水势的诱导作用及其时间效应。结果发现,茉莉酸甲酯处理使蒙古扁桃叶片木质素含量增加,中脉周围的晶细胞数量增多,水势下降;这些变化和茉莉酸甲酯的浓度及处理后时间有关。  相似文献   

13.
沙棘主要蛀干害虫危害特性及种群动态变化   总被引:4,自引:0,他引:4  
沙棘木蠹蛾主要危害沙棘栽植树的根部和干部,红缘天牛同时危害沙棘栽植树和根蘖苗的干部,已成为宁夏沙棘林中两大主要蛀干害虫。通过对沙棘树不同高度范围内沙棘木蠹蛾幼虫和红缘天牛幼虫的调查发现,沙棘木蠹蛾幼虫主要分布在地下根部和干部40~120cm的范围内,而红缘天牛主要分布在树干40~120cm的高度内。红缘天牛幼虫数量随时间变化大致呈现向树干上部增加的变化趋势。沙棘木蠹蛾幼虫数量随时间变化呈现明显的向根部转移的趋势,7月末,有近98%的幼虫在根部危害。之后,由于初孵幼虫的大量出现,使干部幼虫数量急剧增加,越冬前,虽然有部分转移至根部危害,但到9月末仍有近43%的幼虫停留在树干40~120cm处越冬,直到次年越冬结束。沙棘木蠹蛾和红缘天牛在沙棘树干部40cm以上危害位置的较大重叠,必然造成它们在时间、空间和营养上的激烈竞争。  相似文献   

14.
The present study deals with effects of flooding depth on growth, morphology and photosynthesis in Alnus japonica species thorough one field study and two controlled experiments. In the field study performed in Kushiro Mire, Hokkaido Island, Japan, tree heights and stem diameters decreased with an increase in water depth accompanied with the reduction of soil redox potential. In contrast, the rate of multiple stems per individual tree increased. In the controlled experiments for seedlings flooding suppressed the shoot elongation and biomass increment in roots. However, diameter increment around water levels, epicormic shoot development and adventitious root formation were enhanced in flooded seedlings. The photosynthetic rate and stomatal conductance of flooded seedlings also were lowered with an increase in flooding depth. The recovery of the reduced photosynthetic rate and stomatal conductance occurred simultaneously with the advancement of adventitious root formation in the flooded seedlings. These results indicate the importance of a series of morphological changes occurring on stems around water levels in flood tolerance in A. japonica species.  相似文献   

15.
Plantlets of Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) had lower rates of net CO(2) uptake and higher rates of root respiration than greenhouse-grown seedlings. They also had a lower photosynthetic efficiency at high photosynthetic photon flux density, and exhibited significant depression of CO(2) uptake under water stress. The ability of plantlets to utilize high irradiance and to photosynthesize under conditions of water stress was enhanced by the presence of many roots. Carbon dioxide uptake rate increased with increasing root number, and decreased as the needle surface area/root surface area ratio decreased. High root number, low needle surface area/root surface area ratio, and elongated roots were associated with low apparent rates of shoot and root respiration.  相似文献   

16.
We assessed stem burial and adventitious root formation of two late-successional species, Abies mariesii and A. veitchii, in central Japan. In a plot (5 × 5 m), all seedlings between 8 and 24 cm tall were excavated: six A. mariesii seedlings in soil, and six and four A. veitchii seedlings in soil and on logs, respectively. For each sampled seedling, the number of terminal bud scars (TBS) was counted on the aboveground and belowground stems. Stem length was measured, and divided into aboveground and belowground stems. Among the three groups (A. mariesii seedlings in soil and A. veitchii seedlings in soil or on logs), there was no significant difference in height or total root weight (sum of adventitious roots and primary roots), but diameter at ground level and number of TBS were significantly different. Counting TBS on the aboveground stem of seedlings in soil underestimated seedling age, whereas the estimate was much closer to the true age for seedlings on logs. Seedlings in soil formed more adventitious roots than seedlings on logs. A large proportion of the stem was buried in humus for seedlings in soil, while most of the stem was not buried in humus for seedlings on logs. These results suggest that substrate affects adventitious root formation, the formation of which is important to shade tolerance. Thus, our preliminary results suggest that consideration of adventitious root formation is necessary to understand seedling bank dynamics and estimate seedling ages of these Abies species in spatially heterogeneous old-growth subalpine forests.  相似文献   

17.
18.
Ozone exposure decreases belowground carbon allocation and root growth of plants; however, the extent to which these effects persist and the cumulative impact of ozone stress on plant growth are poorly understood. To evaluate the potential for plant compensation, we followed the progression of ozone effects, with particular emphasis on the development of new roots. Ponderosa pine (Pinus ponderosa Dougl. ex Laws.) seedlings were exposed to ozone for 2 years. Following removal of the seedlings from ozone, root growth was assessed to characterize the carry-over effects on new root production, and carbohydrate concentrations were measured to determine if allocation strategies differed among ozone treatments. Four months after removal from ozone, dormant seedlings had significantly lower starch concentrations in stems, coarse roots and fine roots than control seedlings. Following root flushing, starch concentrations in all seedlings decreased, with ozone-treated seedlings containing significantly less starch, sucrose, fructose, glucose and total monosaccharides than control seedlings. There was some evidence that stem starch was mobilized to compensate partially for the lower concentrations of root starch in ozone-treated seedlings; however, there was significantly less new root production in seedlings previously exposed to ozone for 2 years than in control seedlings. Early senescence of older needle age classes, perhaps resulting in inadequate available photosynthate, may be responsible for the reduction in new root production during the year following exposure to ozone. Stored carbohydrate reserves, which were depleted in seedlings previously exposed to ozone, were insufficient to compensate for the ozone-induced reduction in canopy photosynthate. We conclude that there are carry-over effects of ozone exposure on ponderosa pine seedlings, including an enhanced potential for seedling susceptibility to other stresses even in respite years when ozone concentrations are low.  相似文献   

19.
One-year-old Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) and lodgepole pine (Pinus contorta Dougl.) seedlings were grown for 17 weeks in 100-cm deep, 7.8-liter containers. Two Douglas-fir provenances, one from a wet and one from a dry site in coastal British Columbia, and two lodgepole pine provenances, one from a wet and one from a dry site in interior British Columbia, were grown in wet (522% water content) or dry (318% water content) peat/vermiculite soil in a factorial design. Each container was sealed so that water loss occurred only through the seedling. Five harvests were made at three to five week intervals and water use, dry matter increment, root length and root weight were determined at each harvest. Stomatal conductance and shoot water potentials were measured during the last 12 weeks of the experiment. Lodgepole pine seedlings had greater dry matter production, water use, stomatal conductance and new root length than Douglas-fir seedlings. New root weight of lodgepole pine seedlings exceeded that of Douglas-fir seedlings during the last five weeks of the experiment, and specific root length (root length per unit root weight) of new roots was higher for lodgepole pine seedlings throughout the experiment. Douglas-fir seedlings showed higher water use efficiency (WUE) than lodgepole pine seedlings, and both species showed higher WUE in the dry soil treatment. Douglas-fir seedlings had lower water potentials and higher water uptake rates per unit of new root length than lodgepole pine seedlings, although water uptake rates per unit of root dry weight showed little difference between species. Soil water treatment influenced specific root length of new roots, water uptake per unit of new root length, and WUE in Douglas-fir seedlings more than in lodgepole pine seedlings.  相似文献   

20.
When conifers such as Picea abies Karst. (Norway spruce) are attacked by insects or pathogens, they often respond by producing increased quantities of terpenoid oleoresin. This response can be mimicked in young P. abies seedlings by treatment with methyl jasmonate (MJ). In this study, we determined the effects of MJ on terpenoids and other chemical defenses of mature P. abies, and investigated whether this treatment protected trees against attack by the blue-stain fungus Ceratocystis polonica (Siem.) C. Moreau, the most important fungal associate of the spruce bark beetle Ips typographus L. Methyl jasmonate treatment induced the formation of traumatic resin ducts in the developing xylem, enhanced resin flow and stimulated increased accumulation of monoterpenes, sesquiterpenes and diterpene resin acids. However, only minor changes were detected in terpene composition in response to MJ treatment and no changes in soluble phenolic concentration were measured. There was much variability in the timing and degree of response to MJ among clones. The observed chemical and anatomical changes in response to MJ treatment were correlated with increased resistance to C. polonica, suggesting that terpenoid oleoresin may function in defense against this pathogen.  相似文献   

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