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1.
Prairie hay supplemented with various amounts of corn and soybean meal was fed to steers in two experiments. Effects of supplementation on hay OM intake, digestion, and ruminal fermentation and kinetics were measured. A preliminary study was conducted to attain accurate values for OM intake and digestibility of prairie hay to be used in ration formulation using the NRC (1996) level 1 model. Ten steers (284 +/- 9 kg) given ad libitum access to chopped prairie hay (75% NDF, 6% CP) were supplemented with dry-rolled corn (0.75% of BW/d) plus soybean meal (0.25% of BW/d). Hay OM intake was 1.85% of BW and hay OM digestibility was 48%. Based on results from the preliminary study, eight ruminally cannulated beef steers (317 +/- 25 kg) received a sequence of eight different supplementation combinations (2 x 4 factorial arrangement of treatments). These supplements consisted of dry-rolled corn at either 0 or 0.75% of BW (DM basis) daily combined with one of four amounts of added soybean meal to provide between 0 and 1.3 g of degradable intake protein (DIP)/kg of BW. After supplements had been fed for 10 d, feces were collected for 4 d. Intake of hay and total OM increased quadratically (P < 0.01) in response to added DIP with or without supplemental corn. Hay OM digestibility increased quadratically (P = 0.03) as DIP was added when corn was fed in the supplement. Intake of digestible OM was greater (P < 0.01) with than without corn supplementation. Increasing DIP increased (P < 0.01) digestible OM intake regardless of whether corn was fed. Inadequate ruminally degraded protein in grain-based supplements decreased forage intake, digestibility, and energy intake of cattle fed low-quality prairie hay. Providing adequate supplemental DIP to meet total diet DIP needs seemed to overcome negative associative effects typically found from supplementing low-quality forages with large quantities of low-protein, high-starch feeds.  相似文献   

2.
This experiment was designed to investigate the effects of different concentrations (0, 0.33, 0.66, 0.99, and 1.32 g/L) of a twin-strain of Saccharomyces cerevisiae live cells on in vitro mixed ruminal microorganism fermentation of corn starch, soluble potato starch, and sudangrass hay (60.5%, DM basis) plus concentrate mixture (39.5%, DM basis). Ruminal fluid was collected from two dairy cows, mixed with phosphate buffer (1:2), and incubated (30 mL) anaerobically at 38 degrees C for 6 and 24 h with or without yeast supplement, using 200 mg (DM basis) of each substrate. Medium pH, ammonia-N, and numbers of protozoa were unaffected (P = 0.38) by yeast cells in all substrates. Molar proportion of acetate was unchanged (P = 0.56) with cornstarch and soluble potato starch, but increased quadratically (P = 0.02) with hay plus concentrate by treatment. Addition of yeast cells caused a linear increase of total VFA (P = 0.008) in all substrates. Excluding the soluble potato starch, supplementation of S. cerevisiae resulted in a quadratic increase of propionate (P = 0.01), with a quadratic decrease (P = 0.04) of acetate:propionate. When soluble potato starch was used as a substrate, a linear increase (P = 0.006) of the molar proportion of propionate and a quadratic decrease (P = 0.007) in acetate:propionate was observed by treatment. Molar proportion of butyrate was unchanged (P = 0.35) with cornstarch and soluble potato starch, whereas it decreased linearly (P = 0.007) with hay plus concentrate by yeast cell supplementation. When cornstarch and soluble potato starch were used as a substrate, minor VFA were decreased (P = 0.05) by treatment. Accumulation of lactate was linearly decreased by treatment (P = 0.007) in all substrates. During incubation with hay plus concentrate, IVDMD was linearly increased (P = 0.006), whereas production of methane (linear; P = 0.02) and accumulation of hydrogen was decreased (quadratic; P = 0.005) by treatment after 24 h. These results showed that a twin strain of S. cerevisiae live cells stimulated in vitro mixed ruminal microorganism fermentation with decreased lactate, and a small decrease of methane and hydrogen with hay plus concentrate.  相似文献   

3.
In vitro digestion and growth studies were conducted to evaluate the effects of level of soybean oil inclusion in forage-based diets. In Exp. 1, diets were bromegrass hay (H), bromegrass hay and corn-soybean meal supplement (C), C with 3% added soybean oil (O3), and C with 6% added soybean (O6). Diets containing supplements were formulated to be isonitrogenous and isocaloric. Treatment means were compared using a single-degree-of-freedom contrast (H vs C, O3, and O6) and orthogonal polynomial contrasts within diets C, O3, and O6. Diet H had the lowest (P = .0003) IVDMD and a linear decline (P = .0001) in IVDMD was observed from C to O6, but 24-h IVDMD disappearance was greatest (P = .001; quadratic) for O3. Total VFA increased from C to O3 and then decreased from O3 to O6 (quadratic; P = .001), and acetate:propionate ratio decreased linearly (P = .0001) from C to O6. Changes in long-chain fatty acids reflected biohydrogenation by ruminal microbes; however, only 18:3 was hydrogenated to the same extent across all diets. In Exp. 2, 36 Angus x Gelbvieh heifers (260.0 +/- 6.0 kg initial BW) were individually fed C, O3, or O6 as mixed rations for 104 d. Diets were formulated to be isonitrogenous and provide ADG of .91 kg. Feed efficiency and ADG was greatest (P < .02; quadratic) for O3 heifers. Serum NEFA increased linearly (P = .02) and serum glucose (P = .02), cholesterol (P = .002), and GH (P = .04) showed a quadratic response to level of dietary soybean oil. Plasma proportions of 16:0, 16:1, 18:0, and 18:1 increased quadratically (P < .03), and 18:2 increased linearly (P < .001) from C to O6. In Exp. 3, 42 Angus x Gelbvieh heifers (288.7 +/- 6.6 kg initial BW) were divided into six pens (two pens/treatment) in a randomized complete block designed experiment. Rations were delivered as hay plus a top-dressed supplement (C, O3, or O6). Heifers fed O3 conceived 10 d earlier (quadratic; P = .06) than heifers fed C and O6. Other production estimates did not differ (P > or = .10) among dietary treatments. Inclusion of soybean oil at 3% of a forage-based diet increased total VFA, many blood metabolites, ADG, and feed efficiency, and it decreased time to conception. Adding soybean oil as 3% of a forage-based diet is an acceptable feeding strategy for developing beef heifers.  相似文献   

4.
The objectives of this study were to evaluate the influence of supplemental whole flaxseed level on intake and site and extent of digestion in beef cattle consuming native grass hay. Nine Angus heifers (303 +/- 6.7 kg of BW) fitted with ruminal and duodenal cannulas were used in a triplicated 3 x 3 Latin square. Cattle were given ad libitum access to chopped native grass hay (9.6% CP and 77.5% NDF, OM basis). All animals were randomly allotted to 1 of 3 experimental treatments of hay plus no supplement (control); 0.91 kg/d whole flaxseed (23.0% CP, 36.3% NDF, and 25.5% total fatty acid, OM basis); or 1.82 kg/d whole flaxseed on a DM basis. Supplemental flaxseed tended to decrease (linear, P = 0.06) forage OM intake. However, total OM intake did not differ (P = 0.29) with increasing levels of flaxseed. Total duodenal OM flow increased (linear, P = 0.05) with additional flaxseed in the diet, and no differences (P = 0.29) were observed for microbial OM flow. True ruminal OM disappearance was not affected (P = 0.14) by supplemental flaxseed. Apparent lower tract OM digestibility increased (linear, P = 0.01) with level of whole flaxseed. Apparent total tract OM digestibility was not different (P = 0.41) among treatments. Nitrogen intake increased (linear, P < 0.001) with supplemental flaxseed. In addition, total duodenal N flow tended (P = 0.08) to increase with additional dietary flaxseed. However, true ruminal N digestibility did not differ (P = 0.11) across treatment. Supplemental whole flaxseed did not influence ruminal (P = 0.13) or total tract (P = 0.23) NDF digestibility. Ruminal molar proportion of propionate responded quadratically (P < 0.001) with increasing levels of whole flaxseed. An increase in the duodenal supply of 18:3n-3 (P < 0.001), total unsaturated fatty acids (P < 0.001), and total fatty acids (P < 0.001) was observed with additional dietary whole flaxseed. Apparent postruminal 18:3n-3 disappearance tended to decrease (P = 0.07) as intake of flaxseed increased. Overall, the inclusion of 1.82 kg/d of flaxseed does not appear to negatively influence nutrient digestibility of a forage-based diet and therefore can be used as an effective supplement to increase intestinal supply of key fatty acids important to human health.  相似文献   

5.
Four ruminally and duodenally cannulated crossbred beef steers (397+/-55 kg initial BW) were used in a 4 x 4 Latin square to evaluate the effects of increasing level of field pea supplementation on intake, digestion, microbial efficiency, ruminal fermentation, and in situ disappearance in steers fed moderate-quality (8.0% CP, DM basis) grass hay. Basal diets, offered ad libitum twice daily, consisted of chopped (15.2-cm screen) grass hay. Supplements were 0, 0.81, 1.62, and 2.43 kg (DM basis) per steer daily of rolled field pea (23.4% CP, DM basis) offered in equal proportions twice daily. Steers were adapted to diets on d 1 to 9; on d 10 to 14, DMI were measured. Field pea and grass hay were incubated in situ, beginning on d 10, for 0, 2, 4, 8, 12, 16, 24, 36, 48, 72, and 96 h. Ruminal fluid was collected and pH recorded at -2, 0, 2, 4, 6, 8, 10, and 12 h after feeding on d 13. Duodenal samples were taken for three consecutive days beginning on d 10 in a manner that allowed for a collection to take place every other hour over a 24-h period. Linear, quadratic, and cubic contrasts were used to evaluate the effects of increasing field pea level. Total DMI and OMI increased quadratically (P = 0.09), whereas forage DMI decreased quadratically (P = 0.09) with increasing field pea supplementation. There was a cubic effect (P < 0.001) for ruminal pH. Ruminal (P = 0.02) and apparent total-tract (P = 0.09) NDF disappearance decreased linearly with increasing field pea supplementation. Total ruminal VFA concentrations responded cubically (P = 0.008). Bacterial N flow (P = 0.002) and true ruminal N disappearance (P = 0.003) increased linearly, and apparent total-tract N disappearance increased quadratically (P = 0.09) with increasing field pea supplementation. No treatment effects were observed for ruminal DM fill (P = 0.82), true ruminal OM disappearance (P = 0.38), apparent intestinal OM digestion (P = 0.50), ruminal ADF disappearance (P = 0.17), apparent total-tract ADF disappearance (P = 0.35), or in situ DM disappearance of forage (P = 0.33). Because of effects on forage intake and ruminal pH, field peas seem to act like cereal grain supplements when used as supplements for forage-based diets. Supplementing field peas seems to effectively increase OM and N intakes of moderate-quality grass hay diets.  相似文献   

6.
Nine Angus x Gelbvieh heifers (average BW = 347 +/- 2.8 kg) with ruminal and duodenal cannulas were used in a split-plot designed experiment to determine the effects of soybean oil or corn supplementation on intake, OM, NDF, and N digestibility. Beginning June 8, 1998, heifers continually grazed a 6.5-ha predominantly bromegrass pasture and received one of three treatments: no supplementation (Control); daily supplementation of cracked corn (Corn) at 0.345% of BW; or daily supplementation (0.3% of BW) of a supplement containing cracked corn, corn gluten meal, and soybean oil (12.5% of supplemental DM; Oil). Soybean oil replaced corn on a TDN basis and corn gluten meal was included to provide equal quantities of supplemental TDN and N. Three 23-d periods consisted of 14 d of adaptation followed by 9 d of sample collections. Treatment and sampling period effects were evaluated using orthogonal contrasts. Other than crude fat being greater (P = 0.01) for supplemented heifers, chemical and nutrient composition of masticate samples collected via ruminal evacuation did not differ (P = 0.23 to 0.56) among treatments. Masticate NDF and ADF increased quadratically (P < or = 0.003) and N decreased linearly (P = 0.0001) as the grazing season progressed. Supplementation did not influence (P = 0.37 to 0.83) forage OM intake, total and lower tract OM digestibility, ruminal and total tract NDF digestibility, or total ruminal VFA; however, supplemented heifers had lower ruminal molar proportions of acetate (P = 0.01), higher ruminal molar proportions of butyrate (P = 0.007), and greater quantities of OM digested in the rumen (P = 0.10) and total tract (P = 0.02). As the grazing season progressed, total tract OM and N and ruminal NH3 concentrations and NDF digestibility decreased quadratically (P < or = 0.04). Microbial N flow (P = 0.09) and efficiency (P = 0.04) and postruminal N disappearance (P = 0.02) were greater for Control heifers and declined linearly (P < or = 0.02) as the grazing season advanced. Depressed microbial N flow seemed to be more pronounced for Oil than for the Corn treatment. Although total digestible OM intake increased with supplementation, metabolizable protein supply was reduced in supplemented heifers. Therefore, feeding low levels of supplemental grain with or without soybean oil is an effective strategy to increase dietary energy for cattle grazing high-quality forages, but consideration should be given to reduced supply of metabolizable protein.  相似文献   

7.
Hereford x Angus steers were used in a 13-treatment, four-period, incomplete Latin square design to examine the effects of starch and degradable intake protein (DIP) supplements on forage utilization and ruminal function. Steers were given ad libitum access to low-quality hay (4.9% CP) and were not supplemented (NS) or received different amounts of starch (cornstarch grits; 0, .15, and .3% of initial BW) and DIP (Na-caseinate; .03, .06, .09, and .12% of initial BW) administered via ruminal fistulae in a 3 x 4 factorial arrangement of treatments. Supplemented steers consumed more (P < .01) forage OM, total OM, NDF, and digestible OM (DOM) than NS steers. Forage OM, total OM, NDF, and DOM intakes increased linearly (P < .01) as the amount of supplemental DIP increased. The addition of starch to supplements linearly decreased ( P < .01) the intake of forage OM, NDF, and DOM. The digestion of DM, OM, and NDF increased linearly (P < .01) with supplemental DIP and decreased linearly (P < or = .06) with supplemental starch. Particulate and liquid passages generally increased with DIP; however, starch level influenced the nature of the response (P = .03 and .06, respectively). Similarly, ruminal acid detergent-insoluble ash content generally decreased as starch increased, but the effect was dependent on DIP level (P < .01). Supplementation increased (P < .01) ruminal NH3 and total VFA and decreased (P < .01) ruminal pH relative to NS. All treatments supported average pH values in a range (6.3 to 6.7) unlikely to inhibit fibrolytic bacteria. Ruminal NH3 concentration increased quadratically (P = .03) with DIP and decreased linearly (P = .02) with starch. As DIP increased, total VFA concentration increased linearly (P = .02). Providing supplemental DIP to steers fed low-quality forage increased OM intake and digestion, whereas addition of starch to supplements decreased forage intake and digestion.  相似文献   

8.
Two experiments were conducted at two locations to determine the effects of dietary CP concentration and source on performance, carcass characteristics, and serum urea nitrogen (SUN) concentrations of finishing beef steers. British x Continental steers were blocked by BW (357 +/- 28 and 305 +/- 25 kg initial BW; n = 360 and 225; four and five pens per treatment in Exp. 1 and 2, respectively). Steam-flaked corn-based diets were arranged in a 3 x 3 factorial with three CP concentrations (11.5, 13, or 14.5% of DM) and three sources of supplemental CP (N basis): 100% urea; 50:50 blend of urea and cottonseed meal; or 100% cottonseed meal. Steers in both experiments were initially implanted with Ralgro and reimplanted with Revalor-S on d 56. Performance and carcass data were pooled across locations. Crude protein concentration x source interactions were not observed (P = 0.22 to 0.93) for performance and carcass data. Crude protein concentration affected ADG (P = 0.02) and carcass-adjusted (to a common dressing percent within location) ADG quadratically (P = 0.06). Increasing the concentration of supplemental urea linearly increased carcass-adjusted ADG and G:F (P < 0.05) and carcass-adjusted G:F (P < 0.001). Dry matter intake was not affected (P = 0.93) by either CP concentration or source. Hot carcass weight (HCW; P = 0.02), LM area (P = 0.05), and dressing percent (P = 0.03) increased linearly with increasing urea concentration, whereas increasing CP concentration quadratically affected HCW (P = 0.02), with a maximum at 13% CP. Differences in backfat thickness and yield grade were negligible across treatments. Neither marbling score nor percentage of carcasses grading USDA Choice was affected by CP concentration or source. At all times measured, SUN concentrations increased (P < 0.05) with increasing CP concentration, but effects of CP source were small and variable across time. Results indicate that increasing CP concentrations from 11.5 to 13% slightly increased ADG and carcass-adjusted ADG, whereas increasing the proportion of supplemental urea increased carcass-adjusted ADG, G:F, and carcass-adjusted G:F and increased HCW, LM area, and dressing percent. A CP concentration above 13% seemed detrimental to ADG and HCW. Serum urea N increased over time, with CP concentration having a greater effect than CP source.  相似文献   

9.
Six ruminally cannulated Holstein steers (initial BW = 189 +/- 11 kg) housed in metabolism crates were used in a 6 x 6 Latin square to study effects of ruminal ammonia load on Leu utilization. All steers received a diet based on soybean hulls (2.7 kg of DM/d), ruminal infusions of 200 g of acetate/d, 200 g of propionate/d, and 50 g of butyrate/d, as well as an abomasal infusion of 300 g of glucose/d to provide energy without increasing microbial protein supply and an abomasal infusion of a mixture (238 g/d) of all essential AA except Leu. Treatments were arranged as a 3 x 2 factorial and included Leu (0, 4, or 8 g/d) infused abomasally and urea (0 or 80 g/d) infused ruminally. Abomasal Leu infusion linearly decreased (P < 0.05) both urinary and fecal N excretions and linearly increased (P < 0.05) retained N, but the decreases in urinary N excretion in response to Leu tended (P = 0.07) to be greater, and the increases in retained N in response to Leu were numerically greater in the presence of the urea infusion. Although urea infusions increased (P < 0.05) plasma urea concentrations, urinary N excretions, and urinary urea excretions, retained N also was increased (P < 0.05). The efficiency of deposition of supplemental Leu ranged from 24 to 43% when steers received 0 or 80 g of urea/d, respectively. Under our experimental conditions, increasing ammonia load improved whole-body protein deposition in growing steers when Leu supply was limiting.  相似文献   

10.
Two in vitro digestion experiments were conducted to evaluate the influence of the novel urease inhibitor N-(n-butyl) thiophosphoric triamide (NBPT) on in vitro urea kinetics, substrate digestion, and fermentation characteristics. In Exp. 1, in vitro incubations were conducted in 50-mL test tubes containing .25 g of ground fescue hay to which 0, 6.5, 13, 26, or 52 mg of NBPT in a buffered ruminal fluid innoculum was added. Tubes were incubated in triplicate at 39 degrees C and replicated on consecutive days, with NH3 N and urea concentrations measured at 0, 10, 30, 60, 120, 240, and 360 min. Samples for VFA analysis were collected at 6 h, and incubations were continued through 48 h to estimate true digestibility (based on NDF analysis). Increasing the dose of NBPT tended (P < .12) to linearly depress the rate of urea hydrolysis and decreased (P < .0004) subsequent NH3 N formation. Although total VFA concentration at 6 h increased linearly (P < .03), acetate:propionate and estimated true digestibility decreased (P < .01) with increasing NBPT concentration. In Exp. 2, we compared in vitro urea kinetics and digestion of forage-only or mixed forage-grain substrates in response to addition of NBPT. In vitro incubations were conducted in 50-mL test tubes containing either .5 g of ground fescue hay or .5 g of a ground fescue hay and ground corn mixture (50:50, DM basis) to which 0, 6.5, 13, 26, or 52 mg of NBPT in a buffered ruminal fluid innoculum was added. Tubes were incubated in triplicate at 39 degrees C and replicated on consecutive days, with NH3 N and urea concentrations measured at 0, .5, 1, 2, 4, 8, 12, 24, and 48 h. At 48 h, samples for VFA analysis were collected and true digestibility (based on NDF analysis) was estimated. No (P > .10) NBPT dose x substrate interactions were detected. Increasing the dose of NBPT depressed (P < .003) the rate of urea hydrolysis and subsequent NH3 N formation, regardless of substrate. Although total VFA concentration was unaffected (P > .10), the acetate:propionate and estimated true digestibility decreased (P < .002) with higher NBPT addition. In both experiments, the rate of urea degradation was not different (P > .20) from zero for the 26 and 52 mg NBPT treatments, indicating that nearly complete inhibition of urease had been achieved. We conclude that NBPT can be used to reduce the rate of NH3 N release from dietary urea and, thereby, offers the potential to improve nonprotein nitrogen utilization in ruminants.  相似文献   

11.
Our objectives were to compare the effects of sources of supplemental N on ruminal fermentation of dried citrus pulp (DCP) and performance of growing steers fed DCP and bahiagrass (Paspalum notatum) hay. In Exp. 1, fermentation of DCP alone was compared with that of isonitrogenous mixtures of DCP and solvent soybean meal (SBM), expeller soybean meal (SoyPLUS; SP), or urea (UR). Ground (1 mm) substrates were incubated in buffered rumen fluid for 24 h, and IVDMD and fermentation gas production kinetics and products were measured. Nitrogen supplementation increased (P < 0.10) ruminally fermentable fractions, IVDMD, pH, and concentrations of NH3 and total VFA, but reduced the rate of gas production (P < 0.10) and the lag phase (P < 0.01). Supplementation with UR vs. the soy-based supplements increased ruminally fermentable fractions (P < 0.05) and concentrations of total VFA (P < 0.10) and NH3 (P < 0.01), but these measures were similar (P > 0.10) between SBM and SP. In Exp. 2, 4 steers (254 kg) were fed bahiagrass hay plus DCP, or hay plus DCP supplemented with CP predominantly from UR, SBM, or SP in a 4 x 4 Latin square design, with four 21-d periods, each with 7 d for DMI and fecal output measurement. Nitrogen-supplemented diets were formulated to be isonitrogenous (11.9% CP), and all diets were formulated to be isocaloric (66% TDN). Intake and digestibility of DM, N, and ADF were improved (P < 0.05) by N supplementation. Compared with UR, the soy-based supplements led to greater (P < 0.05) DM and N intakes and apparent N and ADF digestibilities. Plasma glucose and urea concentrations increased (P < 0.10) with N supplementation and were greater (P < 0.01) for the soy-based supplements than for UR. Intake, digestibility, and plasma metabolite concentrations were similar (P > 0.1) for SBM and SP. In Exp. 3, 24 steers (261 kg) were individually fed bahiagrass hay plus DCP (control), or hay plus DCP supplemented with CP predominantly from UR or SBM. Over 56 d, DMI and ADG were greatest (P < 0.05) in steers fed SBM. Nitrogen supplementation increased (P < 0.05) DMI, ADG, and G:F. However, SBM supplementation produced greater (P < 0.05) DMI and ADG and similar (P > 0.05) G:F compared with UR supplementation. We conclude that supplemental N is important to optimize ruminal function and performance of growing steers fed forage diets supplemented with DCP. Diets with supplemental N mainly from SBM improved diet digestibility and animal performance beyond that achieved by UR.  相似文献   

12.
Three experiments were conducted to determine the effects of phytase, excess Zn, or their combination in diets for nursery pigs. In all experiments, treatments were replicated with five to seven pens of six to seven pigs per pen, dietary Ca and available P (aP) levels were decreased by 0.1% when phytase was added to the diets, excess Zn was added as ZnO, a basal level of 127 mg/kg of Zn (Zn sulfate) was present in all diets, and the experimental periods were 19 to 21 d. In Exp. 1, pigs (5.7 kg and 18 d of age) were fed two levels of phytase (0 or 500 phytase units/kg) and three levels of excess Zn (0, 1,000, or 2,000 ppm) in a 2 x 3 factorial arrangement. Added Zn linearly increased ADG and ADFI during Phase 1 (P = 0.01 to 0.06), Phase 2 (P = 0.02 to 0.09), and overall (P = 0.01 to 0.02). Gain:feed was linearly increased by Zn during Phase 1 (P = 0.01) but not at other times. Dietary phytase decreased ADG in pigs fed 1,000 or 2,000 ppm Zn during Phase 2 (Zn linear x phytase interaction; P = 0.10), did not affect (P = 0.27 to 0.62) ADFI during any period, and decreased G:F during Phase 2 (P = 0.01) and for the overall (P = 0.07) period. Plasma Zn was increased by supplemental Zn (Zn quadratic, P = 0.01) but not affected (P = 0.70) by phytase addition. In Exp. 2, pigs (5.2 kg and 18 d of age) were fed two levels of phytase (0 or 500 phytase units/kg) and two levels of Zn (0 or 2,000 ppm) in a 2 x 2 factorial arrangement. Supplemental Zn increased ADG and G:F during Phase 2 (P = 0.02 to 0.09) and overall (P = 0.07 to 0.08), but it had no effect (P = 0.11 to 0.89) on ADG during Phase 1 or ADFI during any period. Phytase supplementation increased ADG (P = 0.06) and G:F (P = 0.01) during Phase 2. Gain:feed was greatest for pigs fed 2,000 ppm Zn and phytase (Zn x phytase interaction; P = 0.01). Bone (d 20) and plasma Zn (d 7 and 20) were increased (P = 0.01) by added Zn but not affected (P = 0.51 to 0.90) by phytase. In Exp. 3, pigs (5.7 kg and 19 d of age) were fed a basal diet or the basal diet with Ca and aP levels decreased by 0.10% and these two diets with or without 500 phytase units/kg. Supplemental phytase had no effect (P = 0.21 to 0.81) on growth performance. Reduction of dietary Ca and aP decreased (P = 0.02 to 0.08) ADG, ADFI, and G:F for the overall data. These results indicate that excess dietary supplemental Zn increases ADG and plasma and bone Zn concentrations. Dietary phytase did not affect plasma or bone Zn concentrations.  相似文献   

13.
Five dual-flow fermentors (700 mL) were used to determine the effects of eastern gamagrass (Tripsacum dactyloides [L.] L.) diets on microbial metabolism by mixed rumen cultures. Fermentors were incubated with filtered ruminal contents and allowed to adapt for 4 d to diets followed by 3 d of sample collection. Five dietary treatments were tested: 1) gamagrass hay (GH) + no corn (GHNC), 2) gama grass silage (GS) + no corn (GSNC), 3) GS + low corn (GSLC), 4) GS + medium corn (GSMC); and 5) GS + high corn (GSHC). The experiment was conducted as a randomized complete block design with five treatments and three replications. Total VFA concentrations were not affected by diets. Corn addition linearly decreased (P < 0.001) molar proportion of acetate. In contrast, molar proportion of propionate was reduced in GSLC (cubic effect, P < 0.001) but remained similar across other diets. Corn supplementation linearly increased molar proportion of butyrate (P < 0.001). The acetate + butyrate-to-propionate ratio was highest in cultures offered GSLC (cubic effect, P < 0.001) but similar across other diets. Feeding GSNC resulted in a higher ruminal pH compared with GHNC (P < 0.03). Increasing the level of corn supplementation in GS linearly decreased culture pH (P < 0.001). All diets resulted in similar methane production, with the exception of GSMC, which lowered methane output (quadratic effect, P < 0.004). Total substrate fermented to VFA and gas tended to be greater with GHNC than with GSNC (P < 0.06) and linearly increased with the addition of corn (P < 0.004). Neutral detergent fiber digestibility was similar between GH and GS and was not affected by supplemental corn. Microbial N flow increased in cultures offered GSHC (quadratic effect, P < 0.02). Corn supplementation at the medium and high level linearly decreased C 18:0 (P < 0.02) and increased trans-C18:1 (P < 0.004). Including corn at the high level with GS did not have a detrimental effect on fermentation in dual-flow fermentors.  相似文献   

14.
Four ruminally and duodenally cannulated beef steers (492 +/- 30 kg) were used in a 4 x 4 Latin square design to evaluate the effect of undegradable intake protein (UIP) supplementation on intake, digestion, microbial efficiency, in situ disappearance, and plasma hormones and metabolites in steers fed low-quality grass hay. The steers were offered chopped (10.2 cm in length) grass hay (6.0% CP) ad libitum and 1 of 4 supplements. Supplemental treatments (1,040 g of DM daily), offered daily at 0800, were control (no supplement) or low, medium, or high levels of UIP (the supplements provided 8.3, 203.8, and 422.2 g of UIP/ d, respectively). The supplements were formulated to provide similar amounts of degradable intake protein (22%) and energy (1.77 Mcal of NE(m)/kg). Blood samples were taken at -2, -0.5, 1, 2, 4, 8, 12, and 24 h after supplementation on d 1 (intensive sampling) and at -0.5 h before supplementation on d 2, 3, 4, and 5 (daily sampling) of each collection period. Contrasts comparing control vs. low, medium, and high; low vs. medium and high; and medium vs. high levels of UIP were conducted. Apparent and true ruminal OM and N digestion increased (P < 0.03) in steers fed supplemental protein compared with controls, but there were no differences (P > 0.26) among supplemental protein treatments. There were no differences (P > 0.11) among treatments for NDF or ADF digestion, or total ruminal VFA or microbial protein synthesis. Ruminal pH was not different (P = 0.32) between control and protein-supplemented treatments; however, ruminal pH was greater (P = 0.02) for supplementation with medium and high compared with low UIP. Daily plasma insulin concentrations were increased (P = 0.004) in protein-supplemented steers compared with controls and were reduced (P = 0.003) in steers fed low UIP compared with steers fed greater levels of UIP. Intensive and daily plasma urea N concentrations were increased (P < 0.01) in protein-supplemented steers compared with controls and increased (P < 0.02) for intensive and daily sampling, respectively, in steers supplemented with medium and high UIP compared with low UIP. Supplemental protein increased apparent and true ruminal OM and N digestion, and medium and high levels of UIP increased ruminal pH compared with the low level. An increasing level of UIP increases urea N and baseline plasma insulin concentrations in steers fed low-quality hay.  相似文献   

15.
Two trials were conducted to evaluate the effects of whole shelled corn supplementation on intake and digestibility of low-quality meadow hay by cattle. In Trial 1, four ruminally cannulated steers (avg BW 418 kg) were used in a latin square design with four treatments (no corn or corn fed at .25%, .50% or .75% of BW). Hay DMI decreased (P = .002) linearly .43 g for each gram of increase in corn intake. In contrast, total diet DMI increased (P = .001) linearly as level of corn supplementation increased. Apparent DM digestibility of the diet increased (P = .026) linearly, whereas hay DM digestibility was not affected (P greater than .05) by supplemental corn, although the DM digestion coefficient for hay alone was 24% higher than for hay fed with corn at .75% of BW. Ruminal ammonia concentration and pH were not affected (P greater than .05) by treatments; however, ratio of acetate:propionate and acetate:butyrate responded (P less than or equal to .097) quadratically to increased corn supplementation. Corn supplementation resulted in a cubic (P less than or equal to .081) decrease in meadow hay NDF disappearance from nylon bags suspended in the rumen at all incubation intervals after 4 h. In Trial 2, 45 crossbred, nonlactating, pregnant cows (avg BW 474 kg) were used in a completely randomized design with three treatments (no corn, .91 and 1.81 kg corn.hd-1.d-1). Cow performance was not altered (P greater than .05) by treatments.(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   

16.
Two experiments were conducted to investigate the effects of the addition of Zn to high quality diets on ruminal fermentation and hydrolysis of urea in vitro and in vivo. In Experiment 1, single effluent continuous culture fermentors were maintained on alfalfa hay-based diets with or without added Zn (400 ppm in total diet) for 4 d. On day 5, 2 g urea were added to the fermentors, and determination of concentrations of VFA and NH4+ in the fluid followed for 8 h. The addition of Zn did not affect concentrations of total VFA or NH4+ in the fluid from the fermentors. In Experiment 2, four non-lactating, Holstein cows previously fitted with ruminal cannulas were used in a switchback-designed experiment. Cows were fed the same diets used in the in vitro experiment at restricted (5.71 kg DM) intakes. The addition of Zn to these diets had no impact on ruminal NH4+ concentrations or concentrations of total VFA in ruminal fluid. However, the addition of Zn to diets did change proportions of individual VFA in ruminal fluid. The proportion of propi-onate tended to increase, and the proportion of butyrate tended to decrease, when Zn was added to the diets. These data indicate that supplemental Zn was ineffective in preventing urea hydrolysis when fed with high quality diets but was effective in altering the fermentation pattern.  相似文献   

17.
Supplemental corn grain for steers grazing native rangeland during summer   总被引：1，自引：0，他引：1  
Effects of supplemental corn grain on forage OM intake (FOMI), digesta kinetics, ruminal fermentation patterns, in vitro OM digestibility (IVOMD), and in situ OM digestion were examined in steers grazing summer blue grama rangeland in northeastern New Mexico during July and August 1988. Sixteen ruminally cannulated steers (average BW 507 kg) were allotted to four treatments and individually fed whole-shelled corn at 0, .2, .4, and .6% of BW in a complete random design with repeated measurements over time. Forage OMI decreased linearly (P = .02) with increasing levels of supplemental corn; however, a tendency toward greater FOMI, as well as faster particulate and fluid passage, was observed when corn was fed at .2% of BW compared with 0, .4, or .6% of BW. Molar proportions of butyrate increased (P less than .10) but molar proportions of acetate and propionate, ruminal pH, and total VFA concentration did not change (P greater than .10) with added corn. Added corn linearly decreased (P less than .10) ruminal ammonia N concentrations in July, but patterns were inconsistent in August. A cubic response (P less than .05) for in situ OM disappearance with added corn was noted after 24, 72, and 96 h of incubation. Supplemental whole corn fed at .2% of BW had no detrimental effects and tended to increase FOMI. However, supplemental corn fed at .4 or .6% of BW decreased FOMI compared with 0 or .2% of BW.  相似文献   

18.
Effects of supplemental glucose and degradable intake protein on nutrient digestion and urea kinetics in steers (Bos taurus) given ad libitum access to prairie hay (4.7% CP) were quantified. Six ruminally and duodenally cannulated steers (initial BW 391 kg) were used in a 4 × 4 Latin square with 2 extra steers. Treatments were arranged as a 2 × 2 factorial and included 0 or 1.2 kg of glucose and 240 or 480 g of casein dosed ruminally once daily. Each period included 9 d for adaptation, 4 d for total fecal and urine collections, and 1 d for ruminal and duodenal sampling. Jugular infusion of (15)N(15)N-urea with measurement of enrichment in urine was used to measure urea kinetics. Glucose reduced forage intake by 18% (P < 0.01), but casein did not affect forage intake (P = 0.69). Glucose depressed (P < 0.01) total tract NDF digestion. Glucose supplementation decreased ruminal pH 2 h after dosing, but the effect was negligible by 6 h (treatment × time; P = 0.01). Providing additional casein increased the ruminal concentration of NH(3), but the increase was less when glucose was supplemented (casein × glucose; P < 0.01). Plasma urea-N was increased (P < 0.01) by additional casein but was reduced (P < 0.01) by glucose. Microbial N flow to the duodenum and retained N increased (P ≤ 0.01) as casein increased, but neither was affected by glucose supplementation. Urea-N entry rate increased (P = 0.03) 50% with increasing casein. Urinary urea-N excretion increased (P < 0.01) as casein increased. The proportion of urea production that was recycled to the gut decreased (P < 0.01) as casein increased. Glucose supplementation decreased (P < 0.01) urinary urea excretion but did not change (P ≥ 0.70) urea production or recycling. The amount of urea-N transferred to the gut and captured by ruminal microbes was less for steers receiving 480 g/d casein with no glucose than for the other 3 treatments (casein × glucose interaction, P = 0.05), which can be attributed to an excess of ruminally available N provided directly to the microbes from the supplement. Overall, the provision of supplemental glucose decreased forage intake and digestibility. Increasing supplemental casein from 240 to 480 g/d increased urea production but decreased the proportion of urea-N recycled to the gut.  相似文献   

19.
Seven ruminally cannulated Holstein steers (194 +/- 16 kg) housed in metabolism crates were used in a 6 x 6 Latin square, with one additional steer, to study effects of ruminal ammonia load on methionine (Met) use. All steers received a diet based on soybean hulls (2.6 kg DM/d), ruminal infusions of 200 g/d of acetate, 200 g/d of propionate, and 50 g/d of butyrate, as well as abomasal infusion of 300 g/d of glucose to provide energy without increasing microbial protein supply, and abomasal infusions of a mixture (248 g/d) of all essential AA except Met. Treatments were arranged as a 3 x 2 factorial and included urea (0, 40, or 80 g/d) infused ruminally to supply metabolic ammonia loads and Met (2 or 5 g/d) infused abomasally. Supplementation with the greater amount of Met decreased (P < 0.05) urinary N excretion from 68.8 to 64.8 g/d and increased (P < 0.05) retained N from 22.0 to 27.5 g/d. Urea infusions linearly increased (P < 0.05) urinary N excretions, plasma urea concentrations, and urinary urea excretions, but retained N was not affected. The efficiency of deposition of supplemental Met, calculated by assuming that Met deposition is 2.0% of protein deposition (6.25 x retained N), ranged between 18 and 27% when steers received 0 or 80 g/d of urea, respectively. There were no (P > or = 0.40) effects of treatments on serum insulin or IGF-I concentrations. In our model, increasing ammonia load did not affect whole-body protein deposition in growing steers when Met was limiting.  相似文献   

20.
The effect of altering supplementation frequency on host N balance and key N transactions in the ruminal ecosystem were monitored. Four ruminally fistulated beef steers (BW = 513 kg; SEM = 6.5) were used in a 2 x 2 crossover design with two periods and two supplementation frequency treatments. Supplementation frequencies were 2 and 7 d/wk. Steers were fed tallgrass prairie hay (73.1% NDF, 5.3% CP) ad libitum. Supplement (42% CP; DM basis) was fed at 0.36% BW/d to steers supplemented 7 d/wk. Steers supplemented 2 d/wk received the same amount of supplement per week, but it was equally split among the two supplementation events. Steers supplemented 7 d/wk had higher forage (P < 0.02) and total digestible OM intake (P < 0.06), total N intake, fecal N excretion, and N retention. Although both supplementation frequencies were characterized by positive N balance, the decrease in N retention in the steers supplemented 2 d/wk was due to higher (P < 0.01) urinary N loss. Ruminal fluid was sampled at 0, 2, 4, 6, 12, 24, 48, and 72 h after supplementation beginning on a day when both treatments were supplemented. Frequency x hour interactions (P < 0.02) were observed for ruminal N metabolism criteria. Counts of peptide- and AA-fermenting bacteria peaked at 2 h and returned to nadir by 12 h for steers supplemented 7 d/wk. Steers supplemented 2 d/wk peaked at 6 h with a greater population and returned to nadir at 48 h. Ruminal ammonia concentrations followed a similar trend. Specific activity of ammonia production was lower (P < or = 0.05) immediately after supplementation for steers supplemented 2 d/wk, but by 12 h was the same as for 7 d/wk steers. Ruminal peptides and free AA peaked at 2 h for steers supplemented 2 d/wk and were generally higher (P < or = 0.05) during the first 6 h compared with steers supplemented 7 d/wk. Total VFA concentration was not different (P = 0.35) due to supplementation frequency. Frequency x hour interactions (P < 0.01) were observed for all molar proportions of VFA. The molar proportion of acetate and acetate:propionate ratio were lower (P < 0.01) and the molar proportions of propionate and butyrate were higher for steers supplemented 2 d/wk from 4 h to 24 h. In conclusion, forage use and N balance improved with supplementation 7 d/wk, but supplementation 2 d/wk was associated with some desirable shifts in select ruminal events that may contribute to moderating potential negative impacts of supplementing infrequently.  相似文献   

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