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1.
Controlled-environment chambers were used to study the effects of elevated CO2 concentrations on biological N fixation, N mineralization and C decomposition in rice soil. In three chambers, CO2 concentration was maintained at 353ᆣ/396ᆫ µmol mol-1 (day/night; ambient CO2), while in another three, CO2 was maintained at 667ᆸ/700ᆽ µmol mol-1 (day/night; elevated CO2) throughout the growing season. Rice (var. Nipponbare) seedlings were grown under either ambient or elevated CO2 concentrations, and then transplanted into the soils in the corresponding chambers. At different growth stages, soil samples were taken from surface (0-1cm) and sub-surface (1-10cm) layers at the centre of four hills, then sieved (<1 mm) to remove root residues. Fresh soil was used to measure N fixation activity (using the acetylene reduction assay), NH4+ content and organic C. Separate sets of soil samples were transferred to serum bottles and anaerobically incubated at 30°C for 30 days to measure potential rates of N mineralization and C decomposition. Under an elevated atmospheric CO2 concentration, acetylene reduction activity significantly increased in the surface soil layer during the early cultivation stages and in the sub-surface soil layer during the latter part of cultivation. There was no difference in the amount of NH4+ in fresh soils between elevated and ambient CO2 chambers, while the rate of N mineralization was increased by elevated CO2 during the latter part of cultivation. Soils from the elevated CO2 chambers had obviously higher rate of C decomposition than that from the ambient CO2 chambers. CH4 production gradually increased with the growth of rice plants. These results suggest that elevated CO2 affected biological N fixation, N mineralization and C decomposition in submerged rice soil during the different growth stages of rice.  相似文献   

2.
The objectives of this study were to determine the variability in mineralization of dairy manure N, to determine if N mineralization can be predicted by compositional factors or by near- or mid-infrared reflectance spectroscopy. Dairy manures (n =107) were collected from farms in Maryland, Virginia, Pennsylvania, New York, and Connecticut. The composition of these manures ranged from 14 to 386 g dry matter kg-1, 0.9 to 9.5 kg total N/m3, and 0.3 to 4.7 kg NH4+-N/m3. Manure-amended soil was aerobically incubated at 25°C and concentrations of NH4+-N and NO3--N were determined at day 2 and day 56. The manures were highly variable in their N mineralization characteristics, ranging from a net mineralization of 54.9% to a net immobilization of 29.2% of the organic N. When compositional parameters were individually regressed against percentage mineralized organic N, the highest correlation coefficient (r) was 0.164. A stepwise regression of all 11 variables yielded a maximal r of 0.486. These results suggest that the availability of dairy manure organic N is highly variable and that the availability cannot be predicted from simple compositional parameters. No relationship was found between near-infrared spectral characteristics and N mineralization suggesting that no simple relationship exists between N mineralization and compositional characteristics. There appears to be some potential for the use of mid-infrared for determining the mineralization potential of manures.  相似文献   

3.
A field study was conducted to investigate the effects of N fertilization on soil N pools and associated microbial properties in a 13-year-old hoop pine (Araucaria cunninghamii) plantation of southeast Queensland, Australia. The treatments included: (1) control (without N application); (2) 300 kg N ha-1 applied as NH4NO3; and (3) 600 kg N ha-1 as NH4NO3. The experiment employed a randomized complete block design with four replicates. Soil samples were taken approximately 5 years after the N application. The results showed that application of 600 kg N ha-1 significantly increased concentrations of NH4+-N in 0-10 cm soil compared with the control and application of 300 kg N ha-1. Concentrations of NO3--N in soil (both 0-10 cm and 10-20 cm) with an application rate of 600 kg N ha-1 were significantly higher compared with the control. Application of 600 kg N ha-1 significantly increased gross N mineralization and immobilization rates (0-10 cm soil) determined by 15N isotope dilution techniques under anaerobic incubation, compared with the control. However, N application did not significantly affect the concentrations of soil total C and total N. N application appeared to decrease microbial biomass C and N and respiration, and to increase the metabolic quotient (qCO2) in 0-10 cm soil, but these effects were not statistically significant. The lack of statistical significance in these microbial properties between the treatments might have been associated with large spatial variability between the replicate plots at this experimental site. Spatial variability in soil microbial biomass C and N was found to relate to soil moisture, total C and total N.  相似文献   

4.
Nitrification inhibitors specifically retard the oxidation of NH4+ to NO2- during the nitrification process in soil. In this study, the influence of soil properties on the nitrification-inhibiting effect of 3,4-dimethylpyrazole-phosphate (DMPP), a newly developed nitrification inhibitor, has been investigated. Based on short-term incubation experiments, where the degradation of DMPP could be largely disregarded, the oxidation of the applied NH4+ was more inhibited in sandy soils compared with loamy soils. The influence of soil parameters on the relative NO2- formation could be described by a multiple regression model including the sand fraction, soil H+ concentration and soil catalase activity (R2=0.62). Adsorption studies showed that the binding behaviour of DMPP was influenced markedly by soil textural properties, viz. the clay fraction (r2=0.61). The adsorption of DMPP was found to be an important factor for the inhibitory effect on NH4+ oxidation in a short-term incubation (r2=0.57). It is concluded that the evaluated soil properties can be used to predict the short-term inhibitory effect of DMPP in different soils. The significance of these results for long-term experiments under laboratory and field conditions needs further investigation.  相似文献   

5.
Net N mineralization was studied in three different forest sites (Belgium): a mixed deciduous forest with oak (Quercus robur L. and Quercus rubra L.) and birch (Betula pendula Roth) as dominant species, a deciduous stand of silver birch (Betula pendula) and a coniferous stand of Corsican pine (Pinus nigra ssp. Laricio). The organic (F + H) layer and mineral soil at different depths (0-10, 10-20 and 20-30 cm) were sampled at three locations in the mixed deciduous forest (GE, GF1, GF2), at one location in the silver birch stand (SB) and one in the Corsican pine stand (CP). All samples were incubated over 10 weeks under controlled temperature and moisture conditions. The net N mineralization rates in the organic and upper mineral layer (0-10 cm) were found to be significantly different from the other layers and accounted for 66-95% of the total mineralization over the first 30 cm. Net N mineralization rates in the organic layer ranged from 4.2 to 27.3 mg N m-2 day-1. Net N mineralization and nitrification rates were positively correlated. For the mineral soil, net N mineralization rates decreased with depth and the upper 10 cm showed significantly higher rates, ranging from 8.9 to 33.5 mg N m-2 day-1. The rates of the 10-20 cm and 20-30 cm sublayers were similar, ranging from 1.2 to 7.4 mg N m-2 day-1. The net N mineralization rates for the total mineral layer (0-30 cm) ranged from 17.4 mg N m-2 day-1 (SB) to 36.1 mg N m-2 day-1 (CP). Both from PCA and multiple regression analysis, we could conclude that net N mineralization rates were closely related to the initial mineral N content (Ninitial). Furthermore, significant correlations were observed between the net N mineralization rate, the total carbon (TC) and NH4+-N content for the mineral layers and between net N mineralization rate, total nitrogen (TN), hemicellulose content and C/N for the organic layers.  相似文献   

6.
Volatilization of NH3 from soil is a major N-loss mechanism that reduces the efficiency of applied N fertilizers, and causes environmental pollution. Strategies are needed to reduce the loss. The influences of dicyandiamide (DCD), farmyard manure (FYM) and irrigation on NH3 volatilization from an alluvial soil in rice (Oryza sativa L.)-wheat (Triticum aestivum L.) cropping system was studied using the acid trap method. The loss of NH3 in the rice-wheat system ranged from 38.6 kg N ha-1 from the unfertilized soil to 69.0 kg N ha-1 in the treatment with urea+DCD. Substitution of 50% N provided through urea by FYM reduced NH3-N volatilization by 10% in rice and wheat as compared to the urea treatment. Application of DCD increased NH3 volatilization in wheat by 7% but in rice it had no effect. The irrigation level had no effect on NH3 volatilization in rice but fewer irrigations with fewer splits of N in wheat resulted in higher NH3 volatilization. Application of DCD and FYM with urea had similar effects on grain yield and N uptake by rice and wheat as that of the urea treatment. The study showed that integrated use of organic manure and chemical fertilizer has the potential to reduce the loss of N due to volatilization and thereby minimize environmental pollution. Nitrification inhibitors, which are reported to be useful in increasing the N-use efficiency by reducing the leaching and denitrification losses of N, however, may increase N loss due to volatilization.  相似文献   

7.
外加碳、氮对土壤氮矿化、固定与激发效应的影响   总被引：9，自引：2，他引：9  
本文利用14C和15N对中国生黄绵土(坡地黄绵土)、菜园黄绵土和瑞典耕作草甸土的土壤氮矿化、固定与激发效应进行了研究。结果表明,外加碳、氮能促进土壤氮的矿化、固定与激发效应;促进作用的大小次序为外加NH4-15N大于外加NO3-15N,外加葡萄糖+NH4-15N大于外加葡萄糖+NO3-15N,外加麦秸+NH4-15N大于外加麦秸+NO3-15N,外加葡萄糖+NH4-15大于外加麦秸+NH4-15,外加葡萄糖+NO3-15N大于外加麦秸+NO3-15N;低肥力土壤高于高肥力土壤。在本文中提出了土壤净矿化氮的激发效应、土壤生物固定氮激发效应和土壤总矿化氮的总激发效应的概念,认为土壤氮的总激发效应更能反映土壤氮激发效应的实质。  相似文献   

8.
Restoration of CH4-oxidation activities of desiccated paddy soils and the NH4+ effect after watering were investigated in laboratory incubations. Fresh paddy soil collected from an intermittently flooded rice field in Wuxi, Jiangsu province, showed a parabolic relationship between CH4-oxidation activity and soil moisture with an optimum CH4-oxidation rate at 71% water-holding capacity (WHC), while the paddy soil collected from a permanently flooded rice field in Yingtan, Jiangxi province, showed a much smaller CH4-oxidation ability, which increased exponentially with soil moisture increasing from 28% WHC to 95% WHC at an initial CH4 concentration of ~2,200 µl l-1 and at room temperature (25°C). CH4-oxidation ability was inversely related to N2O emission and related positively with CO2 emission in response to the change in soil moisture. Desiccated paddy soils lost their CH4-oxidation abilities. However, this was recovered after the soils were re-watered. The restoration of CH4-oxidation ability was directly dependent upon soil moisture and the rate of its restoration increased with increasing soil moisture content from 40% to 90% WHC. Addition of NH4Cl at rates of 0-3.57 µmol g-1 soil inhibited the restoration of CH4-oxidation ability significantly (P<0.01), but the inhibitory effect was alleviated by a high soil moisture content. The restoration of CH4-oxidation ability was much slower in the Yingtan soil than in the Wuxi soil. The studies show that the optimum moisture content of paddy soils for CH4 oxidation depends on the methanotrophic bacteria in relation to the prevailing water regime; desiccation damages the CH4-oxidation ability of permanently flooded paddy soil more severely than that of frequently well-drained soils.  相似文献   

9.
During freeze-thaw events, biophysical changes occurring in soils can affect processes such as mineralization, nitrification and denitrification which control inorganic N balances in agro-ecosystems. To evaluate the impact of these climatic events on soil biochemical properties, a study was conducted comparing soil denitrification enzyme activity (DEA), dissolved organic C (DOC) and inorganic N levels before and after the winter season in plots under: (1) continuous corn (Zea mays L.) (CC) with annual chisel plow and disking, (2) corn-soybean (Glycine max L.) (CS) rotation with chisel plow every other year prior to planting soybean, and (3) corn-soybean-wheat (Triticum aestivum L.)/hairy vetch (Vicia villosa Roth) (CSW-V) with ridge tillage during the corn and soybean crops, and dairy manure application during the corn year. Soil cores were collected in late autumn and immediately after spring thaw at 0-5, 5-10, 10-15, and 15-30 cm depths. Regardless of management practices, freeze-thaw events resulted in significant (2-10 times) increases in NH4+-N, NO3--N (P<0.001) and DOC (P<0.01) levels at all soil depths. Following freeze-thaw, DEA remained unchanged in the 5-30 cm depth but dropped significantly (P<0.01) in the 0-5 cm soil layer. In that layer, soils which had been chisel plowed during the previous growing season lost 78-84% of the DEA recorded during the fall, whereas in the plots amended with manure during the previous season, the loss of activity was 40-45%. These data indicate that frequent tillage, compared with manure additions, is more conducive to overwinter loss of DEA in surface layers of soils subject to freeze-thaw cycles.  相似文献   

10.
Effects of earthworms on Zn fractionation in soils   总被引：11，自引：0，他引：11  
Laboratory incubation experiments were conducted to examine the effect of earthworm (Pheretima sp.) activity on soil pH, zinc (Zn) fractionation and N mineralization in three soils. No Zn uptake by earthworms was observed. Zinc addition decreased pH of red soil (soil 1) and hydragric paddy soil (soil 3) by 0.5 and 0.2 unit, respectively, but had no effect on alluvial soil (soil 2). The effect of Zn on soil pH was possibly due to a specific adsorption mechanism between Zn and oxides. Earthworm activity significantly decreased the pH of the red soil, a key factor affecting Zn solubility, but not of the other two soils. Earthworm activity significantly increased DTPA-Zn (DTPA-extractable) and OxFe-Zn (NH2OH-HCl-extractable) in the red soil, but had little effect on other fractions. In the alluvial soil, earthworm activity significantly increased OxFe-Zn but decreased organic-Zn (organic-associated Zn). In the hydragric paddy soil, earthworm activity significantly increased MgCl2-Zn (MgCl2-extractable) and organic-Zn. The level of CaCl2-extractable Zn in all three soils was not affected by earthworm activity. Nitrogen mineralized as a result of earthworm activity was equivalent to 110, 120 and 30 kg N ha-1 in soils 1, 2 and 3, respectively. Zinc added at rates less than 400 mg Zn kg-1 did not seem to affect the activity of N-mineralizing microorganisms. The present results indicated the possibility of increasing the metal bioavailability of relatively low level metal-contaminated soils, with a higher organic matter content, by earthworm inoculation.  相似文献   

11.
Manure N dynamics are affected by manure characteristics, soil factors, and environmental conditions. An incubation experiment was conducted to assess the relationship of these factors. The effects of temperature (11, 18, and 25°C), soil texture (three soils, silt loam to sandy loam), and soil water status (constant at 60% water filled pore space, WFPS, and fluctuating between 30% and 60% WFPS) on net mineralization and nitrification of swine manure N were assessed. Swine manure was applied at an equivalent rate of 350 kg total N ha-1 to 250 g air-dry soil in 2-l canning jars. Subsamples were taken from each jar for NO3- and NH4+ determination when fluctuating moisture treatment dried to 30% WFPS, with sampling continuing through four wet-dry cycles at each temperature. Manure NH4+ was rapidly nitrified to NO3-. The relationship between NO3- accumulation and degree days after application (DDAA, 0°C base) could be described across temperatures using a single pool exponential model for each soil. More NO3- accumulated in coarser-textured soils (150-200 mg N kg-1 soil), compared to 130 mg N kg-1 soil in the silt loam soil. Fluctuating soil water status did not alter estimates of rate and extent of NO3- accumulation, but slowed NH4+ disappearance somewhat.  相似文献   

12.
This study addressed differences between Diplocardia spp. (a native North American earthworm) and Octolasion tyrtaeum (an introduced European species), with respect to behavior, influence on soil microbial biomass, and plant uptake of N in tallgrass prairie soils. We manipulated earthworms in PVC-encased soil cores (20 cm diameter) over a 45-day period under field conditions. Treatments included: (1) control with no earthworms, (2) Diplocardia spp. only, and (3) O. tyrtaeum only. Prior to addition of earthworms, seedlings of Andropogon gerardii (a dominant tallgrass) were established in each core, and a dilute solution of 13C-labeled glucose and 15N-labeled (NH4)2SO4 was added to the soil to facilitate examination of earthworm/microbe/plant interactions. We found that Diplocardia spp. were significantly more active than O. tyrtaeum, and quickly assimilated 13C and 15N from the tracer. Individuals of Diplocardia spp. were present at shallower soil depths than O. tyrtaeum throughout the study. Contrary to expectation, this greater activity of Diplocardia spp. did not result in increased plant productivity. Rather, the activity of Diplocardia spp. was associated with less plant growth and smaller amounts of N acquired by A. gerardii seedlings compared to controls or O. tyrtaeum treatments. We observed few significant influences of earthworm treatments on microbial biomass C or N pool sizes, but the microbial C/N ratio was consistently greater in the presence of Diplocardia spp. relative to O. tyrtaeum. Results of this study indicate that activity of earthworms may enhance competition for N between microbes and plants during the growing season in tallgrass prairie.  相似文献   

13.
施用不同种类氮肥对日光温室土壤溶液离子组成的影响   总被引：4，自引：0，他引：4  
采用土培模拟试验研究了施用不同量的尿素[CO(NH2)2]、碳酸氢铵（NH4HCO3）、硫酸铵[(NH4)2SO4]对培养期间日光温室土壤溶液电导率（EC）和不同离子组成及比例的影响。结果表明,不同氮肥种类对土壤溶液电导率（EC）的影响主要表现在培养的前一周左右,之后不同品种间无明显差异。土壤溶液中NO3--N含量随施氮量和培养时间呈明显的上升趋势,不同氮肥种类NO3--N含量无明显差异;不同氮肥种类处理土壤溶液中NH4+-N含量在培养的前7 d有所差异,之后亦无差异。随着氮肥施用量的增加,日光温室土壤溶液的EC及K+、Na+、Ca2+、Mg2+离子的浓度升高;增施氮肥同时提高了土壤溶液中Ca/K、Mg/K的比值,而对土壤溶液钾的活度比（ARK）无显著影响。说明氮肥施用量是影响土－液界面离子交换的重要因素;由此带来的日光温室土壤盐分累积以及K+、Na+、Ca2+和Mg2+离子的淋失等问题值得关注。  相似文献   

14.
Two field experiments were conducted in 1999 (wet season) and 2000 (dry season) on a Ustic Endoaquerts in central Thailand to examine the impact of rice straw management practices on rice yield, N uptake and fertilizer-N use efficiency. Treatments included a combination of urea broadcast at a rate of 70 kg N haу with either straw or compost which were incorporated at a rate of 5 Mg haу. At maturity of the wet season rice, 15N recovery by the grain was low (11-14%) as well as straw-N derived from labeled N (5-7%). After harvest, 25-29% of applied N still remained in the soil, mainly in the 0 to 5-cm layer. Large amounts of fertilizer-N (53-55%) were lost (unaccounted for) from the soil/plant system during the first crop. Residual fertilizer-N recovery in the second rice crop was less than 3% from the original application. During both fallow seasons NO3m-N remained the dominant form of mineral N (NO3m + NH4+) in the soil but its concentration was low. In the wet season grain yield response to N application was significant (P =0.05). Organic material sources did not significantly change grain yield and N accumulation in rice. In terms of grain yield and N uptake at maturity, there was no significant residual effect of fertilizer-N on the subsequent rice crop. These results indicated that the combined use of organic residues with urea did not decrease total N losses or increase crop yield or uptake of N compared to urea alone.  相似文献   

15.
The mineralisation of green manure from agroforestry trees was monitored with the objective to compare the temporal dynamics of mineralisation of litter from different species. Green manures from five agroforestry tree species were used on a fallow field during the long rainy season of 1997 (March-August) and from two species in the following short rainy season (September-January) in western Kenya. Different methods, i.e. measurements of isotopic ratios of C in respired CO2 and of soil organic matter (SOM) fractions, soil inorganic N and mass loss from litterbags, were used in the field to study decomposition and C and N mineralisation. Soil respiration, with the separation of added C from old soil C by using the isotopic ratio of 13C/12C in the respired CO2, correlated well with extractable NH4+ in the soil. Mineralisation was high and very rapid from residues of Sesbania sesban of high quality [e.g. low ratio of (polyphenol+lignin)/N] and low and slow from low quality residues of Grevillea robusta. Ten days after application, 37% and 8% of the added C had been respired from Sesbania and Grevillea, respectively. Apparently, as much as 70-90% of the added C was respired in 40 days from high quality green manure. Weight losses of around 80%, from high quality residues in litterbags, also indicate substantial C losses and that a build-up of SOM is unlikely. For immediate effects on soil fertility, application of high quality green manure may, however, be a viable management option. To achieve synchrony with crop demand, caution is needed in management as large amounts of N are mineralised within a few days after application.  相似文献   

16.
Lumbricus terrestris' middens contain large concentrations of organic material and have been characterized as microenvironments distinct from the surrounding soil. The direct and indirect consequences of midden formation on nutrient cycling dynamics and organic matter pools in various ecosystem types have not received much consideration. Therefore, we focused on the differences in C and N dynamics between midden and bulk soil samples in four corn (Zea mays L.) agroecosystems, a rotational pasture and a deciduous forest, in June, July and August of 1996, in Ohio, USA. Paired earthworm midden and bulk soil samples were analyzed for mineral N (NH4+-N and NO3--N), dissolved organic N, microbial biomass N (MBN) and carbohydrate C (CarbC). Additionally, coarse litter, fine litter, particulate organic matter, and soil organic matter fractions were separated and analyzed for total C, total N and C:N ratios. Mineral and dissolved N levels were higher in the midden soil relative to those in the bulk soil for all ecosystem types, except for only NO3--N levels in two highly fertilized agroecosystems and in the pasture. MBN, CarbC, and total C and N levels for all organic fractions were significantly greater in the earthworm midden samples relative to these in the bulk samples across all ecosystem types. The plan defined by principal component analysis clearly separated two main groups: (1) includes the forest, the pasture and the less fertilized cornfields and the midden effect is to increase slightly the organic matter content and strongly the inorganic N content, and (2) includes the heavily fertilized agroecosystems and the midden effect is also to increase the organic matter content but to decrease the inorganic N content. We concluded that L. terrestris' middens significantly raised overall soil C and N levels relative to the bulk soil, in a variety of ecosystem types, and, given the abundance of earthworm middens, these macrosites should receive important attention when evaluating nutrient cycling processes at the systems level.  相似文献   

17.
The effects of additions of three wastes from sugar mills on the properties of two acid soils were investigated. The wastes used were boiler ash and filter cake from a conventional mill and fly ash from a new mill, where filter cake is burnt. Additions of each of the wastes raised soil pH, reduced concentrations of exchangeable Al and total and monomeric Al in soil solution and increased maize yields in a pot experiment. Microbial biomass C and the percentage of organic C present as microbial biomass were decreased by additions of wastes but the effect was less marked at the higher rate of addition. Although basal respiration was decreased or unaffected by the lower rate of addition of wastes it was increased by the higher rate. The metabolic quotient increased in the order: control4+ and NO3- accumulated during incubation and arginine ammonification rate were all less at the higher rate of addition of each of the wastes. This was attributed to microbial immobilization of mineral N and arginine due to the wide C/N ratio of light fraction organic matter present in the study soil. It was concluded that all three waste materials were effective liming materials but their effect on soil microbial activity was complex.  相似文献   

18.
Heterotrophic N2-fixing bacteria are a potentially important source of N2 fixation in rice fields due to the moist soil conditions. This study was conducted at eight sites along a geographic gradient of the Yangtze River Plain in central China. A nitrogen-free solid malate-sucrose medium was used to isolate heterotrophic N2-fixing bacteria. Numbers of the culturable N2-fixing bacteria expressed as CFU (colony forming units) ranged between 1.41ǂ.42᎒6 and 1.24ǂ.23᎒8 in the sampled paddy field sites along the plain. Thirty strains with high ARA (acetylene reduction activity) were isolated and purified; ARA of the strains varied from 0.9 to 537.8 nmol C2H4 culture-1 h-1, and amounts of 15N fixed ranged between 0.008 and 0.4866 mg·culture-1·day-1. According to morphological and biochemical characteristics, 14 strains were identified as the genus Bacillus, 2 as Burkholderia, 1 as Agrobacterium, 4 as Pseudomonas, 2 as Derxia, 1 as Alcaligenes, 1 as Aeromonas, 2 as Citrobacter, and 3 strains belonged to the corynebacter-form group.  相似文献   

19.
An experiment was conducted in an Andosol paddy field in Shizukuishi (Iwate Prefecture, Japan) to determine the effects of free-air CO2 enrichment (FACE) on biological N2-fixation activity and soil microbial biomass C at three levels of N application. Rice (Oryza sativa L. cv. Akitakomachi) plants were grown under ambient CO2 or FACE (ambient +200 µmol mol-1 CO2) conditions throughout the growing season with each treatment having four replicated plots. Three levels of N fertilizer (high, standard and low; 15, 9 and 4 g N m-2, respectively) were applied to examine the effect of different N availability under both CO2 conditions. Soil samples were collected at four different times from upper and lower soil layers (0-1-cm and 1-10-cm soil depths, respectively) and analysed for biological N2-fixation (BNF) activity and microbial biomass C (MBC) by the acetylene reduction and chloroform fumigation-extraction methods, respectively. The amounts of chlorophyll-type compounds (Chls), an index of algal growth, and soil available C were also determined. Compared to the ambient CO2 treatment, the FACE treatment had significantly higher BNF activity in both the upper and lower soil layers at ripening only in low-N soil and at harvest at all three levels of N fertilization rates. MBC was significantly increased by FACE in both the upper and lower soil layers from the middle to later period of the growing season compared to the ambient CO2 treatment. The FACE treatment increased the Chls in the upper soil layers at ripening only in low-N soil and at harvest at all three levels of N fertilization rates. The amount of soil available C was not significantly different between FACE and ambient CO2 treatments in both the upper and lower soil layers throughout the cropping season. From these results it can be concluded that the FACE treatment had a significantly positive influence on BNF activity, MBC and Chls at different levels of N fertilization rates in paddy field during the cropping season.  相似文献   

20.
To identify the key soil parameters influencing N2O emission from the wheat-growing season, an outdoor pot experiment with a total of 18 fertilized Chinese soils planted with wheat was conducted in Nanjing, China during the 2000/2001 wheat-growing season. Average seasonal N2O-N emission for all 18 soils was 610 mg m-2, ranging from 193 to 1,204 mg m-2, approximately a 6.2-fold difference between the maximum and the minimum. Correlation analysis indicated that the seasonal N2O emission was negatively correlated with soil organic C (r2=0.5567, P<0.001), soil total N (r2=0.4684, P<0.01) and the C:N ratio (r2=0.4530, P<0.01), respectively. A positive dependence of N2O emission on the soil pH (r2=0.3525, P<0.01) was also observed. No clear relationships existed between N2O emission and soil texture, soil trace elements of Fe, Cu and Mg, and above-ground biomass of the wheat crop at harvest. A further investigation suggested that the seasonal N2O-N emission (E, mg m-2) can be quantitatively explained by E=1005-34.2SOC+4.1Sa (R2=0.7703, n=18, P=0.0000). SOC and Sa represent the soil organic C (g kg-1) and available S (mg kg-1), respectively.  相似文献   

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