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1.
4 horses (Group A) received an oat-hay ration, through 9 months running, while 3 horses (Group B) were fed a barley-hay ration over the same period of time. Blood samples were collected and examined in 4-week intervals. All values considered, no differences were found to exist between the 2 groups with regard to Hb and haematocrit in blood as well as to protein, glucose, sodium, potassium, magnesium, iron fixation capacity, copper, and zinc in blood plasma. The urea level in plasma of Group A was higher than that in Group B, values being 5.76 +/- 0.64 mmol/l and 5.14 +/- 0.72 mmol/l. The same applied to free fatty acids, values being 150 +/- 83 mumols/l in Group A and 95 +/- 42 mumols/l in Group B. Calcium was 3.10 +/- 0.27 mmol/l in Group A and 2.92 +/- 0.26 mmol/l in Group B, while the A-B difference in inorganic phosphorus amounted to 1.62 +/- 0.65 against 1.99 +/- 0.77 mmol/l. Group A was inferior to Group B also with regard to iron in plasma, values being 28.6 +/- 2.42 mumols/l against 32.8 +/- 4.62 mumol/l. Causes underlying these differences are discussed in some detail.  相似文献   

2.
Keeping of piglets on slatted metal floor, without complementary iron supply, caused anaemia within seven days from parturition. Hb levels in the blood dropped from 7.00 +/- 1.16 to 3.47 +/- 0.74 mmol/l. Iron deficit reduced growth and vitality. The iron levels in blood plasma of the piglets tested varied between 5 and 35 mumol/l, immediately after parturition. Intraperitoneal or oral administration of Fe-dextran (150 mg Fe) on the first day after parturition resulted in physiological iron levels in blood plasma, after seven days. Yet, when 14 days had passed, the levels were similar to those recorded from piglets without additional iron supply. Iron bonding capacity of blood plasma increased in piglets of all groups up to the seventh day from birth. Death occurred, on the second day of age, to many piglets with protein levels below 50 g/l plasma. Copper levels in blood plasma of newborn piglets were below those recordable from sows, but they went up by more than three times of their original values up to the seventh day from birth. In piglets, zinc, levels of blood plasma after parturition were higher than those of sows and went up by some 50 percent, within seven days from birth.  相似文献   

3.
Variations of plasma volume (PV) and variations of triglyceride, cholesterol, total lipid, beta-lipoprotein and phospholipid concentrations in the blood plasma were investigated in trials with ten sows, crossbreds of the White Thoroughbred and Landrace breeds, at the age of 2-3 years, kept on a defined diet; the trials were performed before the sows became pregnant and during their pregnancy (days 1-40, 41-60, 61-80, 81-100 and 101-120). The PV in nonpregnant sows is 9.8 +/- 0.33 1. Following a decrease to the values of 7.8 +/- 0.33 l (p less than 0.01) in the first 40 days of pregnancy the plasma volume increases in the successive periods and it makes 15.4 +/- 0.19 l at the end of pregnancy (p less than 0.001). Total lipaemia decreases during pregnancy from 2.80 +/- 0.054 in nonpregnant sows to 2.49 +/- 0.245 g per 1 in sows at the end of pregnancy. Cholesterol concentrations in the blood plasma also decrease from 2.48 +/- 0.53 in nonpregnant ones to 1.88 +/- 0.173 mmol per 1 (p less than 0.001) in sows at the end of pregnancy, beta-lipoprotein concentrations from 3.95 +/- 1.134 in nonpregnant ones to 3.47 +/- 0.199 g per l in sows on days of pregnancy 81-100 (p less than 0.05), phospholipid concentrations in the first 60 days of pregnancy from 1.62 +/- 0.079 before insemination to 1.29 +/- 0.05 mmol per l in sows of 41-60 day pregnancy.(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   

4.
Blood samples were collected from 22 calves in three weight classes (A: 29.3 +/- 0.5 kg, B: 36.0 +/- 1.3 kg, C: 42.7 +/- 3.7 kg), 1-3 minutes after parturition and prior to uptake of foremilk as well as 24-26 hours after parturition. Other blood samples were collected from 45 calves, 5-60 minutes after parturition, and from their mothers, 3-5 or 5-60 minutes from calving. Haematocrit and haemoglobin in the blood of the calves, immediately after parturition, were higher the values recorded from adult cattle. Major differences were found to exist between individual calves regarding total protein of blood plasma. Protein levels in 14 calves were below 50 g/l, within 24-26 hours from parturition. Free fatty acids in blood plasma of calves were lower than those in cattle, 1-3 minutes from parturition, and were higher, 5-60 minutes from parturition. Glucose levels in Group C were higher than those in A and B, 1-3 minutes postpartum. Lactate in Group C was higher than that in B. An account is also given of blood plasma levels of Ca, Pa, Mg, Na, K, Cu, and Zn.  相似文献   

5.
Babesiosis is a common tick borne disease of dogs in tropical and subtropical regions of the world caused by different species of Babesia. The present study aimed to examine erythrocyte lipid peroxide and erythrocytic antioxidant levels in dogs with clinical babesiosis, caused by Babesia gibsoni, and impact of the disease on blood iron, zinc and copper levels. The study was conducted on 10 naturally occurring cases of canine babesiosis with the history of tick infestation, erratic pyrexia, and prolonged illness. Microscopic examination of Giemsa stained peripheral blood smears confirmed B. gibsoni infection in the erythrocytes. Six apparently healthy dogs of different age, sex and breeds, brought for either health checkup or vaccination were used for comparison. Levels of erythrocytic antioxidant enzymes were significantly (P<0.01) higher in sick dogs than those of cytologically negative dogs (catalase: 0.192+/-0.024 units/mg Hb vs 0.074+/-0.004 units/mg Hb; superoxide dismutase: 0.014+/-0.0009 units/mg Hb vs 0.006+/-0.0008 units/mg Hb and lipid peroxide: 6.01+/-0.30 nmol MDA/mg Hb vs 1.89+/-0.10 nmol MDA/mg Hb). The levels of blood micronutrients were significantly low in these dogs (iron: 89.87+/-8.12 microg/g vs 126.44+/-14.65 microg/g; zinc: 3.67+/-1.85 microg/g vs 5.62+/-1.83 microg/g and copper: 0.55+/-0.63 microg/g vs 0.65+/-0.04 microg/g). The study demonstrated oxidative damage in dogs naturally infected with B. gibsoni. Low level of blood iron, zinc and copper seems to have an additional role in the genesis of anaemia and oxidative stress.  相似文献   

6.
In three series of experiments the variations of lactic acid and glucose concentrations were investigated in pregnant sows, crossbreds of the Large White and Landrace breeds, and in their foetuses on the 104th and 113th day of pregnancy, then in farrowing sows of the same breed and their piglets at delivery (birth) and till the 10th day after birth. In nonpregnant sows the value of lactacidaemia is 1.60 +/- 0.08 mmol per 1. In the first twenty days of pregnancy it increases to 2.50 +/- 0.95 mmol per 1 (p less than 0.05). In the following days of pregnancy until the 81st to 100th day the value ranges from 1.5 to 1.7 mmol per 1. In the last twenty days of pregnancy it increases slightly to 2.10 +/- 0.25 mmol per 1. On the 104th and 113th day the values of lactacidaemia in foetuses in vena and arteria umbilicalis make 7.40 +/- 0.41 and 6.80 +/- 0.37, or 8.50 +/- 0.43 and 8.16 +/- 0.21 mmol per 1, respectively. Glucosaemia values are decreasing in pregnant sows in the first forty days, in the following period of pregnancy they are increasing and on the 101st to 120th day of pregnancy they make 5.39 +/- 0.26 mmol per 1. The foetus to dam ratio lactic acid concentrations is 2.3 and 2.1, respectively, on the 104th and 113th days. Lactacidaemia is highest in farrowing sows (3.15 +/- 0.19 mmol per 1), then there is a statistically significant decrease in the value of this characteristic, and on the day after delivery it makes 2.38 +/- 0.66 mmol per 1.(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   

7.
During the course of parturition seven high pregnant sows, crossbreds of the Large White and Landrace breeds, and their 69 piglets being born, were studied for the changes of the total and separate free amino acids in the blood plasma in relation to the time of duration of the parturition, to the sequence of piglets being born in the litter, and to the birth weight of the piglets. The concentration of the total free amino acids in blood plasma is 2.44 +/- 0.182 mmol.l-1 just before parturition; after the birth of the last piglet it decreases insignificantly to 2.17 +/- 0.190 mmol.l-1. The concentration of the total free amino acids in the blood plasma of newborn piglets is always significantly higher than in their mothers (p less than 0.001) and is 4.24 +/- 0.109 mmol.l-1. The rank of each piglet in the sequence of births in litter has no influence on the level of plasma concentration of the total free amino acids amino acidaemia was 4.03 +/- 0.268 mmol.l-1 in the piglets born first and insignificantly changed to 3.99 +/- 0.445 mmol.l-1 of blood plasma in the last piglets born in the litter. The time factor of parturition is also statistically insignificant. However, the differences in the free "amino acids" concentration in the blood plasma between the piglets with the lowest weight and the piglets with a weight of up to 1200 and 1500 g are statistically significant (p less than 0.02). Alanine (0.43 +/- 0.052 mmol.l-1) and glycine (0.43 +/- 0.063 mmol.l-1) constitute the largest proportions of all the 17 amino acids studied in the blood plasma in the high pregnant sows before parturition. The tyrosine concentration in piglets at birth is remarkably high (1.44 +/- 0.035 mmol.l-1); it represent 34 per cent of the total amino acidaemia. Tyrosine, histidine and lysine concentration in the blood plasma is statistically highly significantly higher (p less than 0.001) in newborn piglets than in their mothers. The phenomenon of 6-times higher tyrosinaemia in piglets, compared with the sows is discussed in connection with the weight at birth and with the data on the function and concentration of thyroid hormones in piglets during the first days of their lives.  相似文献   

8.
Our objectives were to 1) establish ionised calcium (ICa), C-terminal PTH and biologically active PTH (intact molecule) concentrations in blood from normal horses, 2) examine the stability of ionised calcium and acid-base values in stored equine heparinised blood and serum and 3) check the applicability of the formulas based on these parameters in certain disease states. Mean +/- s.d. % ionised calcium in heparinised blood of normal Warmbloods was 51 +/- 2.7 (n = 20) of total calcium, range 1.45-1.75 mmol/l (n = 15) at Michigan State University and 1.43-1.69 mmol/l (n = 20) at Utrecht University. Mean +/- s.d. EDTA plasma concentration for intact +/PTH in normal horses measured 0.6 +/- 0.3 pmol/l (n = 11). Both mean serum and the heparinised blood ionised calcium concentrations changed (not significantly) after 102 h storage at room temperature. Six cycles of freezing and thawing did not affect serum ionised calcium concentration significantly. Ionised calcium concentration and pH in heparinised blood of 20 normal Warmbloods were used to calculate the regression equation for the prediction of the adjusted ionised calcium concentration to a pH of 7.4. The linear regression equation found was: adjusted plasma ICa at pH 7.4 mmol/l = -6.4570 + 0.8739 x (measured pH) + 0.9944 x (measured ICa mmol/l). By means of this formula, mean adjusted ionised calcium concentration in heparinised blood calculated was 100% of the actual value given by the analyser in the normal horses. When using this formula in horses with colic or diarrhoea, mean adjusted ionised calcium concentration was underestimated by 0.2 and 0.3%, respectively. Furthermore, to adjust the measured ionised calcium concentration in heparinised blood to a pH of 7.4 in healthy as well as in 2 groups of diseased horses 2 formulas with a good prediction are now available.  相似文献   

9.
Blood was sampled from fattening pigs fixed with the snout loop in the pen as well as during slaughter after control or container transport. Assays were made of haemoglobin (Hb), haematocrit (Hk), glucose concentration (PG), and lactic acid (MS) in the plasma. The mean values and standard deviations of Hb and Hk were 13.97 +/- 1.14 g/100 ml, 48.36 +/- 3.67 per cent, 15.24 +/- 1.12 g/100 ml, 49.83 +/- 4.04 per cent, 15.32 +/- 1.23 g of 100 ml, and 51.08 +/- 3.73 per cent. The means values of PG were between 51 and 101 mg of 100 ml and those of MS between 8 and 16 mMol/l.  相似文献   

10.
Massive dose injections of butyrate, isobutyrate, and isovalerate (1.25 mmol/kg body weight) as well as of physiological saline solutions were applied to 4 groups of lambs aged 8 to 15, 16 to 22, 23 to 29, and 30 to 45 days. Blood samples were drawn prior to infusion as well as 5, 15, 30, 60, 90, 120, and 180 minutes from mid-infusion. Original glucose and insulin levels in the blood plasma of 87 lambs had been 5.24 +/- 0.85 mmol/l or 191 +/- 106 pmol/l, respectively. Infusion of these solutions led to temporary rise in blood plasma levels of glucose, insulin, and free fatty acids. The highest insulin level increase resulted from isovalerate infusion. Total alpha amino nitrogen in blood plasma went down. It appeared to be obvious that lambs, even before their forestomachs came into action, responded to infusion of volatile fatty acids by increase in insulin secretion.  相似文献   

11.
In one study, a comparison was made of the concentrations and ratios of certain blood cations in 2 groups of cows with milk fever, one showing normal consciousness (n = 8) and the other depressed consciousness (n = 24). There were no significant differences in the mean concentrations of serum total calcium, plasma inorganic phosphorus and potassium, erythrocyte sodium and potassium, the serum calcium/serum magnesium ratio or the plasma sodium/erythrocyte sodium ratio. There were significant differences (all P less than 0.05) in the mean (+/- SD) concentrations of serum magnesium and plasma sodium concentrations, and the plasma sodium/serum magnesium ratio of 0.8 +/- 0.28 vs 1.2 +/- 0.37 mmol/l, 155 +/- 3.0 vs 147 +/- 6.4 mmol/l and 180 +/- 40.1 vs 116 +/- 34.1 for normal vs depressed cows, respectively. In a second study, a comparison was made of the concentrations and ratios of the same blood cations in 3 groups of cows in different positions when attended for milk fever, namely standing (n = 6), sternal recumbency (n = 24) and lateral recumbency (n = 31). There were no significant differences between the mean concentrations or ratios of any of the cations.  相似文献   

12.
OBJECTIVES: To evaluate two treatment methods in bitches with primary uterine inertia in relation to blood concentrations of oxytocin, calcium and glucose. METHODS: A prospective study where 27 bitches with primary inertia were randomly allocated to treatment groups I and II. Blood samples were collected before and after treatment. Group I was treated with a combination of intravenous calcium solutions and oxytocin and group II with oxytocin only. If unsuccessful, caesarean sections or additional medical treatment was pursued. RESULTS: Plasma oxytocin concentrations were 35+/-15 pmol/l in group I and 30+/-15 pmol/l in group II. Before treatment, the total serum calcium concentrations were 2.1+/-0.2 mmol/l in both the groups, and blood glucose values were 7.0+/-0.5 mmol/l (group I) and 7.3+/-1.4 mmol/l (group II). The number of bitches subjected to caesarean sections and the number of puppies born did not differ between the two groups. CLINICAL SIGNIFICANCE: This study indicates that low plasma oxytocin levels is a cause of primary inertia in bitches with normal serum calcium concentrations and aggravates the condition in bitches with low calcium levels. Hypoglycaemia was not observed. The outcome of labour did not differ between groups I and II.  相似文献   

13.
The objectives of Exp. 1 were to determine the effects of production system and genotype on pig performance and health. Sows were bred, gestated, farrowed, and lactated in either an intensive indoor or an intensive outdoor production system. The three dam genotypes of pigs used in each environment were PIC Camborough-15 (C-15), PIC Camborough Blue (CB), and Yorkshire x Landrace (YL). All pigs received 100 mg of iron dextran at d 3 of age. Pigs raised in the outdoor unit had higher blood hemoglobin (Hb) concentrations on d 28 of age than pigs raised indoors (11.5 +/- .22 vs 8.16 +/- .26 g/dL, P < .0001). Outdoor-reared pigs had more white blood cells (WBC) on d 3 than indoor-reared pigs (9.7 +/- .38 vs 8.04 +/- .38 cells/microL x 10(3), P < .05), but outdoor pigs had fewer WBC on d 28 of age than indoor-reared pigs (9.8 +/- .5 vs 11.1 +/- .45 cells/microL x 10(3), P < .05). Genetic lines did not differ in plasma immunoglobulin G (IgG) concentrations at 3 or 28 d of age. Environment and age influenced pig Hb levels and WBC numbers. The objectives for Exp. 2 were to determine whether C-15-405 pigs reared outdoors or indoors needed supplemental iron or whether they would receive enough environmental iron, and how the lack of supplemental iron may impact pig Hb and immunity. Indoor and outdoor pigs received either no supplemental iron, 100 mg, or 400 mg of iron dextran on d 3 of age. Blood percentage neutrophils and neutrophil: lymphocyte ratio were lower (P < .05) indoors, and natural killer cell (NK) activity was greater (P < .05) among indoor- than outdoor-reared pigs (NK % cytotoxicity: 15.6 +/- 2.3 vs 9.7 +/- 2.3). Outdoor-reared pigs that received no injected iron had similar Hb at d 28 of age as indoor-reared pigs that received 100 mg of iron dextran (11.1 +/- .36 vs 10.7 +/- .4 g/dL, P = .59). Supplemental iron may not be necessary in an outdoor production system. Outdoor-reared pigs had lower values for some immune measures, but they had similar survival rates as indoor-reared litters.  相似文献   

14.
Equine babesiosis, caused by Babesia equi and transmitted by ticks is of major economic importance in India. The adverse effects which B. equi organism and its metabolites inflict on red blood cells have not been reported. Erythrocytes were analysed for red cell membrane phospholipids, proteins and haemoglobin (Hb) concentration and plasma for malondialdehyde (MDA) in B. equi carrier donkeys before splenectomy (< 1% parasitaemia) and after splenectomy at 1-5, 5-15, 15-50 and >50% parasitaemia. Before splenectomy the mean values of membrane protein, phospholipids, plasma MDA and Hb were found to be 1.63 +/- 0.12 mg/ml PCV, 2.28 +/- 0.9 mg/ml PCV, 3.63 +/- 0.33 nmoles/ml plasma and 11.52 +/- 0.45 g/dl blood respectively. Erythrocyte membrane protein showed a significant increase at and beyond 5-15% parasitaemia, whereas a significant increase in total phospholipids and MDA level was observed at and beyond 50% parasitaemia. Though, a gradual decrease in Hb value was observed at various stages of parasitaemia and there was a sharp fall when parasitaemia reached more than 50%. Examination of blood smears showed phagocytosis of both healthy and infected erythrocytes.  相似文献   

15.
The objective of this prospective study was to elucidate whether amounts of bicarbonate needed for correction of acidosis and normalization of clinical signs are influenced by blood D-lactate concentrations in calves with diarrhoea. In 73 calves up to 3 weeks old with acute diarrhoea and base excess values below -10 mmol/l correction of acidosis was carried out within 3.5-h by intravenous administration of an amount of sodium bicarbonate which was calculated using the formula: HCO (mmol) = body mass (kg) x base deficit (mmol/l) x 0.6 (l/kg). Clinical signs, venous base excess, and plasma D-lactate concentrations were monitored immediately following admission, following correction of acidosis at 4 h and 24 h after admission. The base excess and plasma D-lactate concentrations throughout the study were -17.8 +/- 4.0, -0.4 +/- 0.4, -3.0 +/- 5.5 mmol/l (base excess), and 10.0 +/- 4.9, 9.8 +/- 4.8, 5.4 +/- 3.4 mmol/l (D-lactate) for the three times of examination. Metabolic acidosis was not corrected in more than half of the calves (n = 43) by the calculated amount of bicarbonate, whereas the risk of failure to correct acidosis increases with D-lactate concentrations. The study shows that calves with elevated D-lactate concentrations do not need additional specific therapy, as D-lactate concentrations regularly fall following correction of acidosis and restitution of body fluid volume, for reasons that remain unclear. However, calves with distinct changes in posture and demeanour need higher doses of bicarbonate than calculated with the factor of 0.6 in the formula mentioned above probably because of D-hyperlactataemia.  相似文献   

16.
Five Holstein-Friesian calves, from one sire, with prevalent black hair coat pigmentation were used in the experiment. The mean age was 33 days and the mean live weight 51 kg. The animals were exposed free running without interruption for 12 hours to an artificial ultraviolet light in the range of 280-320 nm. The mean doses of radiation was 179.10(-10) J/h/m. One-spot high-pressure mercury discharge lamps Tesla RVK 400 W were used as a radiation source. The dose rate was estimated from measurements by a spectral photometer with filter UG 2 for absorbtion of visible light located at the height of the back of standing calf. Blood samples were collected immediately before the beginning of treatment and after 5, 12, 24, 48 and 72 hours. The blood plasma aldosterone was measured by radioimmunoassays, the levels of sodium, potassium and calcium in blood plasma by flame spectrophotometry. Double classification variance analysis and evaluation according to the Snedecor F-test, the contrast effect test according to Duncan and regression analysis were used for statistical evaluation. Compared to the first sampling, sodium increased significantly after 5 and 12 hours of exposure (Tab. I) to 138.1 and 138.3 mmol/l, respectively. In the subsequent samplings this trend continued up to 72 hours from the beginning of irradiation (140.5 mmol/l). The potassium level did not change statistically significantly. Owing to an excessive irradiation, the calcium concentration increased significantly. The greatest increase occurred after 12 hours of irradiation (from 2.29 mmol/l to 2.61 mmol/l) and after 36 hours from the end of irradiation (2.70 mmol/l).(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   

17.
母猪饲粮中添加蛋氨酸铁螯合物对预防仔猪贫血的影响   总被引:18,自引:1,他引:18  
本试验研究了妊娠母猪饲粮中添加蛋氨酸铁螯合物对仔猪血液生化指标、生长速度和预防仔猪贫血的影响。试验结果表明在母猪妊娠第93天至哺乳21天饲粮中添加60mg/kg蛋氨酸铁螯合物,仔猪初生时不注射铁剂组的血红蛋白浓度较对照组仔猪提高15.67A%(10.78:9.32g/100ml),但差异不显著(P>0.05):7、21日龄的血红蛋白浓度为10g/100ml左右,铁营养处于适宜水平。试验组仔猪初生时的红细胞含量显著高于对照组(P<0.01),但7、21日龄时则低于对照组(P<0.o1,P<0.05);仔猪的初生重、日增重和35日龄断奶窝重与对照组相似(P>0.05)。仔猪血液生化指标和体重变化说明蛋氨酸铁螯合物可有效地防止仔猪缺铁性贫血的发生。  相似文献   

18.
Blood was collected from a group of ten sheep immediately after removal from pasture and thereafter at regular intervals for 50 hours whilst maintaining them without food but with adequate water. The samples were analysed for parameters normally examined as an aid to the diagnosis of zinc, copper, selenium, cobalt and iodine status. Serum vitamin B12 levels increased with time. Mean plasma zinc concentrations and mean serum concentrations of both T4 and fT4 followed significant parabolic trends. Maximum mean zinc levels (50 +/- 10% higher than initial values) were recorded after 30 hours. T4 and fT4 levels peaked at approximately 26 hours. Zinc levels at two hours were 20 +/- 5% lower than the initial values. Whole blood copper, selenium and glutathione peroxidase levels did not change significantly with time the largest differences were 11 +/- 6%, 5 +/- 4% and 10 +/- 8% respectively.  相似文献   

19.
Experiments were conducted to elucidate the time required was showing in what time for postnatal absorption (within 40 hours from birth) and, occurrence of hypoglycaemic activity of exogenous insulin, when high concentrations of the hormone had been present in sow colostrum before parturition, 5 days after parturition, and, occasionally during lactation. The mean insulin concentration in piglet plasma samples from 11 litters amounted to 116.48 pmol/l +/- 101.11 (n = 115), and the glucose concentration was 4.69 pmol/l +/- 1.65 (n = 115), before insulin loading. Oral insulin loading (1.0 I.U./kg body weight) was applied to 10 litters. The piglet litters were separately tested, at different periods, 1, 2, 3, 5, 10, 15, 20, 25, 30, 40 hours after birth. After insulin loading, the mean hormone concentration in plasma samples increased to 447.11 pmol/l +/- 277.01 (n = 83) (P less than 0.001), and the glucose concentration dropped to 2.55 mmol/l +/- 1.08 (n = 83) (P less than 0.001). Insulin absorption from the alimentary tract to blood stopped completely in piglets of litters 10 and 11 examined in 30 and 40 hours after birth. 22 piglets had separated from litters Nos. 4 to 11 and were given intramuscularly injections of 1.0 I.U. insulin. The average hormone concentration in their plasma samples increased to 645.51 pmol/l +/- 44.2 (n = 22) (P less than 0.001), while their average glucose concentration dropped to 2.82 mmol/l +/- 0.13 (n = 22) (P less than 0.001). 24 piglets with hypoglycaemic symptoms from litters orally loaded with insulin and 1 piglet of the intramuscular group, were reanimated by intraperitoneal administration of glucose.(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   

20.
The effects of two putative stressors relative to the collection of blood, namely the environment of the treatment room and the pain associated with venepuncture, on plasma levels of luteinising hormone (LH), testosterone and cortisol were examined in six trained male experimental dogs. Blood samples were collected from the dogs in a treatment room as well as in the kennels (control), and by venepuncture as well as via an indwelling intravenous catheter (control). No significant influence of either stressor on plasma levels of LH, testosterone or cortisol was found. Plasma concentrations of these hormones varied considerably both between and within dogs. Mean (+/- SEM; n = 6) plasma concentrations were 4.3 +/- 1.0 micrograms/l for LH, 4.6 +/- 1.9 nmol/l for testosterone and 68 +/- 10 nmol/l for cortisol. It was concluded that the putative stressors, the environment of the treatment room and the pain associated with venepuncture, did not significantly influence plasma levels of LH, testosterone or cortisol in trained male experimental dogs. This conclusion implies that under the experimental conditions described, the validity of results will not be affected by the method of blood collection used.  相似文献   

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