共查询到20条相似文献，搜索用时 31 毫秒
1.
试验旨在研究伊犁马催乳素受体(prolactin receptor,PRLR)基因多态性及其与产奶量之间的关联性。以60匹伊犁马为研究对象,选取PRLR基因侧翼区的部分片段,采用PCR-SSCP技术检测其遗传多态性,并与伊犁马的日产奶量进行关联分析,研究PRLR基因的多态性与伊犁马产奶性能的关系。结果显示,PRLR基因侧翼区的2个片段均具有多态性,都存在3种基因型:AA、AB和BB,测序结果显示PRLR-P1的碱基突变位置为g.29764513 G>A,该突变为错义突变,导致其编码的氨基酸由组氨酸变成精氨酸,经关联分析发现,该突变对伊犁马日产奶量有显著影响(P<0.05);PRLR-P2的碱基突变位置为g.30106804 C>A,该突变为同义突变,未导致其编码的氨基酸发生改变,经关联分析发现,该突变对伊犁马日产奶量有显著影响(P<0.05)。伊犁马PRLR基因侧翼区的碱基突变与日产奶量之间存在关联性,且可能是影响伊犁马产奶性能的重要位点。 相似文献
2.
BI Lan-shu XIAO Hai-xia ZANG Chang-jiang HE Mei-sheng TUOHUTI&# Ajide PAREHATIJIANG&# Wufuer YU Xi LIU Wu-jun 《中国畜牧兽医》2017,44(1):180-185
In this study, the single nucleotide polymorphism of prolactin receptor (PRLR) gene and its correlation with the lactation traits in Jiangyue donkeys was tested, and the molecular genetic marker of breeding Jiangyue donkeys was explored.120 individuals were analyzed association of PRLR gene polymorphism with lactation traits in Jiangyue donkeys. Genetic polymorphism of PRLR gene flanking region was detected by PCR-SSCP method. The results showed that there was 1 mutation in PRLR gene and 2 genotypes of AA and AB, and allele A was the dominant allele. The SNP g.29764584 C>G was identified of PRLR gene by sequencing, and it was a synonymous mutation, did not cause amino acid changes. Statistical results indicated that the SNP was significantly associated with average daily milk yield and protein percentage in Jiangyue donkey (P<0.05),there was no significant in the lactin percentage(P>0.05). The mutation might be as crucial DNA genetic marker for lactation performance selection in Jiangyue donkeys. 相似文献
3.
试验旨在研究疆岳驴催乳素受体（prolactin receptor,PRLR）基因多态性及其与泌乳性状间的关系,探寻可用于疆岳驴选育的分子遗传标记。随机选取120头疆岳驴,对疆岳驴的泌乳性能指标进行测定,并运用PCR-SSCP方法对疆岳驴PRLR基因侧翼区进行多态性分析。结果显示,PRLR基因在疆岳驴群体中存在2种基因型：AA和AB,A为优势等位基因;测序结果显示,PRLR基因的碱基突变位置为g.29764584 C>G,该突变为同义突变,没有导致编码的氨基酸发生改变;经关联分析,PRLR基因AB基因型个体平均日泌乳量显著高于AA基因型（P<0.05）,AA基因型个体乳蛋白率显著高于AB基因型（P<0.05）,在乳糖率上差异不显著（P>0.05）。疆岳驴PRLR基因突变与泌乳性状存在关联性,推测PRLR基因可以作为疆岳驴乳用型选育的分子遗传标记之一。 相似文献
4.
试验旨在研究绵羊催乳素受体(Prolactin receptor,PRLR)基因g.38976954C>A位点多态性和可卡因-苯丙胺调控转录肽(CART prepropeptide,CARTPT)基因g.9856267C>A、g.9854141G>C位点多态性与绵羊产羔数之间的关系,为绵羊多羔新品系的选育和高繁殖力机理研究提供参考。采用重测序和Sequenom MassARRAY?誖SNP技术对常年发情(小尾寒羊、湖羊、策勒黑羊)和季节性发情(草原型藏羊、苏尼特羊、滩羊、萨福克羊)的不同绵羊品种PRLR、CARTPT基因进行检测分型,然后与小尾寒羊产羔数关联分析。结果表明:g.38976954C>A位点有CC、CA和AA三种基因型,该位点基因型频率和等位基因频率在常年发情、季节性发情绵羊品种间差异不显著(P>0.05);g.9856267C>A位点存在CC、CA和AA三种基因型,g.9854141G>C位点存在GG、GC和CC三种基因型,这2个位点的基因型频率和等位基因频率在常年发情、季节性发情绵羊品种间差异均极显著(P<0.01)。群体遗传学分析结果表明,g.38976954C>A位点在小尾寒羊、草原型藏羊、滩羊和策勒黑羊中均表现为低度多态(PIC<0.25),在苏尼特羊、湖羊和萨福克羊中均表现为中度多态(0.25A位点在7个绵羊品种中均表现为低度多态(PIC<0.25);g.9854141G>C位点在小尾寒羊、苏尼特羊、草原型藏羊、滩羊、湖羊和策勒黑羊中均表现为低度多态(PIC<0.25),在萨福克羊中表现为中度多态(0.25A位点在7个绵羊品种中均处于哈代-温伯格不平衡状态(P<0.05);g.9856267C>A位点在草原型藏羊和湖羊中处于哈代-温伯格不平衡状态(P<0.05);g.9854141G>C位点在7个绵羊品种中均处于哈代-温伯格平衡状态(P>0.05)。关联分析表明,PRLR基因g.38976954C>A位点不同基因型与小尾寒羊各胎产羔数之间无显著关联(P>0.05),该位点不适用于小尾寒羊多羔性状选育;CARTPT基因g.9856267C>A和g.9854141G>C位点不同基因型与小尾寒羊各胎产羔数之间无显著关联(P>0.05),但这2个位点不同基因型各胎产羔数相差较大,推测该位点可能对产羔有影响。 相似文献
5.
为寻找伊犁马肉质性能的分子标记,试验以38匹伊犁马为材料,测定肉质性状（失水率、熟肉率、剪切力）和肌纤维性状（肌纤维横截面积、肌纤维直径、肌纤维密度）,利用PCR直接测序法检测肌细胞生成素（meyogenin,MyoG）基因外显子1在伊犁马群体中的多态性,并对MyoG基因SNPs不同基因型与肉质、肌纤维性状进行关联分析。结果表明,MyoG基因外显子1检测出5个突变位点,分别为SNP1（g.31187343 A>C）、SNP2（g.31187333 G>A）、SNP3（g.31187132 C>T）、SNP4（g.31187105 C>G）和SNP5（g.31187099 C>T）,其中SNP1为错义突变,碱基A突变为C使得氨基酸由苏氨酸突变为脯氨酸,其他位点均为无义突变。SNP3和SNP4为中度多态位点,SNP1和SNP2为低度多态位点,这4个位点均处于Hardy-Weinberg平衡状态。MyoG基因外显子1中SNP1和SNP4不同基因型个体失水率、熟肉率、肌纤维横截面积、肌纤维直径、肌纤维密度差异显著（P<0.05）;SNP3不同基因型个体熟肉率、肌纤维横截面积、肌纤维密度差异显著（P<0.05）;SNP2不同基因型个体各指标差异均不显著（P>0.05）。综上,伊犁马MyoG基因外显子1检测到5个多态位点,其中SNP1（g.31187343 A>C）、SNP3（g.31187132 C>T）和SNP4（g.31187105 C>G）位点不同基因型对肉质及肌纤维性状有显著影响,这些位点可作为伊犁马肉质性能潜在分子标记。 相似文献
6.
前期研究通过荷斯坦公牛全基因组重测序鉴定到17个奶牛产奶性状候选功能基因,其中,肽基脯氨酸顺反异构酶基因(PIN1)参与甘油三酯代谢、甘油磷脂代谢以及mTOR信号通路,且位于产奶量和乳蛋白量性状QTL区间。为进一步系统分析PIN1基因是否对奶牛产奶性状具有遗传效应,本实验基于40头公牛的基因组DNA混池,采用PCR产物直接测序法对PIN1基因的全部编码区以及上下游调控区2000 bp进行扫描,在内含子2检测到1个SNP位点7:g.14432394G>A,A、G等位基因频率分别为0.4797和0.5203。采用靶向测序基因型技术对北京地区987头中国荷斯坦母牛进行个体基因型检测,对SNP位点7:g.14432394G>A与5个产奶性状进行关联分析。结果表明:在第1泌乳期,SNP 7:g.14432394G>A与产奶量、乳脂量、乳蛋白量和乳蛋白率呈显著或极显著关联(P=0.0001~0.0493);在第2泌乳期,SNP与产奶量、乳脂量、乳脂率和乳蛋白量呈显著或极显著关联(P=0.0001~0.0104);SNP位点7:g.14432394G>A对产奶量、乳脂量、乳蛋白量和乳蛋白率的加性效应或等位基因替代效应均达到显著或极显著。综上,PIN1基因对中国荷斯坦牛的产奶量和乳蛋白、乳脂性状具有显著遗传效应,可作为遗传标记用于基因组选择,以加快遗传进展。 相似文献
7.
为研究水牛蛋白激酶AMP活化的催化亚基α2（protein kinase AMP-activated catalytic subunit alpha 2,PRKAA2）基因多态性,本试验以摩拉水牛和尼里-拉菲水牛基因组DNA为模板,扩增PRKAA2基因外显子4及内含子3部分序列,通过常规测序法检测其SNP并进行遗传多样性分析。结果发现,PRKAA2基因外显子4内存在1个SNP位点（c.462 G>A）,PRKAA2基因内含子3部分序列存在3个SNPs位点（IVS3.557 T>C、IVS3.560 C>T和IVS3.565 G>A）。经遗传多样性分析表明,在c.462 G>A位点的野生纯合型和杂合型比突变纯合型更有优势,IVS3.557 T>C和IVS3.560 C>T位点的突变纯合型为非优势基因型,IVS3.565 G>A位点杂合型为优势基因型。IVS3.565 G>A位点在摩拉水牛群体中处于Hardy-Weinberg非平衡状态;c.462 G>A位点在尼里-拉菲水牛群体中处于Hardy-Weinberg非平衡状态。4个SNPs位点在摩拉水牛群体中均为中度多态;c.462 G>A、IVS3.557 T>C位点在尼里-拉菲水牛群体中为低度多态,IVS3.560 C>T、IVS3.565 G>A位点为中度多态。IVS3.557 T>C位点在两个水牛群体中杂合度较低。说明摩拉水牛IVS3.565 G>A位点和尼里-拉菲水牛c.462 G>A位点的基因型频率和基因频率遗传状态不平衡,尼里-拉菲水牛群体中IVS3.557 T>C位点遗传变异小,选择潜力不高。4个多态位点可以构建5种单倍型,其中T-C-G-G是摩拉水牛群体和尼里-拉菲水牛群体的优势单倍型。综上,本研究检测的摩拉水牛和尼里-拉菲水牛PRKAA2基因上4个SNPs位点可为水牛标记辅助选择育种提供参考。 相似文献
8.
旨在探究脂肪酸结合蛋白(FABP3)基因在黔北麻羊中的遗传多态性,进一步揭示FABP3基因与黔北麻羊生长性状间的关联性,为今后运用分子标记辅助育种方法提高黔北麻羊的生长性状提供理论依据。本研究以120只6月龄黔北麻羊为试验对象,采用PCR产物直接测序技术检测FABP3基因全部外显子区的遗传多态性,并与体重、体高、体斜长、胸围、管围等生长性状进行关联性分析。结果:在黔北麻羊FABP3基因第5外显子和3′非翻译区筛选出2个SNPs位点,分别为g.13665051C>T和g.13664737G>A,其中g.13665051C>T位点为同义突变,产生3种基因型:CC、CT和TT,g.13664737G>A位点产生3种基因型:GG、AG和AA,卡方(χ2)检验结果显示,这2个SNPs位点处于中度多态且在黔北麻羊群体中均未偏离Hardy-Weinberg平衡(P>0.05)。关联分析显示,g.13665051C>T位点中CT基因型个体的体高、体斜长、胸宽和管围显著高于CC和TT基因型个体(P<0.05);CT基因型个体的胸深、胸围显著高于TT基因型个体(P<0.05),g.13664737G>A位点中不同基因型的生长性状指标均未达到差异显著(P>0.05)。因此,推测黔北麻羊FABP3基因外显子5的g.13665051C>T突变位点可能是影响个体生长性状的重要位点。 相似文献
9.
Wang C Liu M Li Q Ju Z Huang J Li J Wang H Zhong J 《Veterinary immunology and immunopathology》2011,139(2-4):229-236
Mannan-binding lectin (MBL), a pattern recognizing serum protein, participates in the innate immune system of mammals as an opsonin. In humans, single-nucleotide polymorphisms (SNPs) in MBL2 gene were found to cause various innate immune dysfunctions. In the present study, we discovered three single-nucleotide polymorphisms of the MBL1 gene in Chinese native cattle and analyzed their associations with milk traits. By screening the genetic variation of MBL1 in 1053 individuals of three Chinese native cattle breeds including China Holstein, Luxi Yellow and Bohai Black using created restriction site–polymerase chain reaction (CRS–PCR), PCR–restriction fragment length polymorphism (PCR–RFLP) and DNA sequencing techniques, three new SNPs, g.855G>A, g.2651G>A and g.2686T>C, were found to have allele frequencies of 0–12.65%, 24.07–42.39% and 56.95–73.68%, respectively. While SNP g.855G>A is located within intron ?, the other two SNPs reside in the exon II region with one mutation being non-synonymous (GTT (Val) > ATT (Ile)) and the other synonymous (GCT (Ala) > GCC (Ala)). Among the 596 Chinese Holstein cattle with at least 3 lactation Dairy Herd Improvement (DHI) records, eight different haplotypes and 19 genotype combinations were detected. Statistical analyses revealed no correlation between either g.855G>A or g.2686T>C and somatic cell score (SCS), however significant association was found between g.2651G>A and SCS, suggesting a possible role of this SNP in the host response against mastitis. Our data also suggested that the combined genotypes of GGC/AAC with the lowest SCS, AAT/AAT with the highest protein content and AGC/AGC with the highest 305-d milk yield were favorable combinations for mastitis resistance and milk production traits. Therefore, GGC/AAC, AAT/AAT and AGC/AGC can be used as possible candidates for marker-assisted selection in the dairy cattle breeding program. 相似文献
10.
X Wang Z Ju J Huang M Hou L Zhou C Qi Y Zhang Q Gao Q Pan G Li J Zhong C Wang 《Veterinary immunology and immunopathology》2012,148(3-4):311-319
Mannose-binding lectin (MBL), a calcium-dependent collagenous lectin, plays an important role in the host immune defence against a wide range of pathogens. There are MBL1 and MBL2 genes which encode the MBL-A and MBL-C proteins, respectively. This study was carried out to investigate the relationship between the variants of the bovine MBL2 gene and milk production traits, mastitis, serum MBL-C levels and hemolytic complement activity in both classical pathway (CH50) and alternative pathway (ACH50) in Chinese Holstein cattle. Four single-nucleotide polymorphisms (SNPs) in the exon 1 of the MBL2 gene in Chinese Holstein cattle and Luxi yellow cattle were identified by the direct sequencing method. The SNP g.201 G>A was identified as a non-synonymous mutation (codon 31, Arg>Gln) at the N-terminus cysteine-rich domain and the SNPs g.234 C>A and g.235 G>A (codon 42) made Pro to Gln at the 1st Gly-X-Y repeat of the collagen-like domain, while the SNP g.244 T>C (codon 45) was identified as a synonymous mutation (Asn>Asn) at the 2th Gly-X-Y repeat of the collagen-like domain. The SNP markers (g.201 G>A, and g.234 C>A) were significantly correlated with somatic cell score (SCS) (P<0.05). The concentration of MBL-C protein in serum ranges from 0.8 to 7.4μg/mL by enzyme-linked immunosorbent assay. Six combinations of different haplotypes from the four SNPs were identified in Chinese Holstein cattle. Statistical analysis revealed that cows with the haplotype combination H4H5 exhibited the lowest SCS. The CH50 value of H4H5 and H5H5 cow are significantly higher than H2H5 haplotype combination (P<0.05). The association analysis results showed that the haplotype combination H4H5 may be used as a tolerance haplotype combination for the bovine mastitis. 相似文献
11.
为了探究PRL基因多态性与鸡产蛋性能的相关性,试验以95只百宜黑鸡为研究对象,利用PCR扩增技术结合直接测序方法对鸡PRL基因SNP位点进行筛选.结果:在试验群体中找到4个SNPs,分别为g.6171 C>T、g.6232 C>G、g.6306 C>T、g.6440 T>C,且4个SNPs均处于第5外显子,g.6171 C>T和g.6232 C>G位点之间属于完全连锁平衡;χ2检验发现,该基因座的位点并不处于Hardy-Weinberg平衡状态;遗传参数分析发现,除了g.6306 C>T属于低度多态,其余位点均属于中度多态;关联分析表明,各SNP位点与产蛋性能之间不存在显著相关关系(P>0.05). 相似文献
12.
作者所在团队前期通过奶牛乳腺上皮组织转录组测序及荷斯坦公牛全基因组重测序研究发现RPL23A和ACACB基因是奶牛乳蛋白和乳脂性状的候选功能基因,本研究旨在探究这两个基因是否对奶牛产奶性状具有显著遗传效应。以北京地区7个牧场的1059头中国荷斯坦母牛为试验群体,采集尾根静脉血并提取基因组DNA,通过飞行时间质谱方法检测SNP位点基因型,利用SAS9.4软件的MIXED过程进行关联分析。结果表明,RPL23A基因的SNP位点g.20146771C>T与第1泌乳期5个产奶性状达到显著或极显著关联(P=0.0001~0.0416),其优势等位基因为T;ACACB基因的g.63878254T>C位点与第1泌乳期产奶量、乳脂量和乳蛋白量呈极显著关联(P<0.01),其优势等位基因为C;g.63962768G>A位点与第1泌乳期产奶量、乳脂量、乳脂率和乳蛋白率关联显著或极显著(P=0.0001~0.0391),其优势等位基因为A。综上,RPL23A基因主要影响中国荷斯坦牛产奶量和乳蛋白,ACACB基因对产奶量和乳脂具有显著遗传效应,3个SNP位点可考虑作为遗传标记用于标记辅助选择培育奶牛高乳蛋白乳脂新品系和选育提高。 相似文献
13.
马毛色是品种鉴定和个体识别的重要依据。马 KIT 基因位于3号染色体,KIT 基因突变影响马毛色及毛色的分布。对德保矮马和哈萨克马69个个体的 KIT 基因21个外显子及部分内含子直接测序,共发现了5个SNPs,其中1个位于5'-UTR区（g.91214T>G）,1个位于内含子20 （g.171356C>G）,另外3个分别位于外显子15、20和21(g.164297C>T;g.170189C>T;g.171471G>A,p.Ala960Thr)。用PCR-RFLP方法对69个个体进行分型,发现外显子15有3种基因型TT、CT、CC;外显子20有3种基因型TT、CT、CC;外显子21有2种基因型GG与GA,且均为野生型占优势。德保矮马 KIT 基因多态性比哈萨克马更丰富。 相似文献
14.
Zhang CL Chen H Wang YH Zhang RF Lan XY Lei CZ Zhang L Zhang AL Hu SR 《Research in veterinary science》2008,85(2):265-268
Serotonin receptor 1B (HTR1B) is one of the 14 different identified serotonin receptors which are involved in the regulation of behaviors such as sleep, fear, aggression, mood, and feeding. The aims of this study were to characterize polymorphisms in the 5' coding and the 3' flanking regions of the bovine HTR1B gene among Chinese Holsteins and to identify the association of HTR1B polymorphisms with milk production performance. SSCP was used to examine the polymorphisms at four loci and the fragments with different SSCP patterns were sequenced. A total of three single nucleotide polymorphisms (SNPs) were detected, among which the 205G>T mutation was found to cause a predicted amino acid change: from Ala to Ser at position 69. The polymorphism of G205T was identified to be associated with milk yield trait. Furthermore, the H1-C genotype was found to be associated with a significant increase in milk yield of 489kg vs. the H1-A genotype (P<0.05). 相似文献
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【目的】 探究磷脂酰肌醇-4,5-二磷酸3-激酶催化亚单位β（PIK3CB）基因多态性及其与中国荷斯坦牛繁殖和产奶性状的关系。【方法】 通过混池测序对中国荷斯坦牛PIK3CB基因进行单核苷酸多态性（SNP）位点筛选,采用竞争性等位基因特异性PCR （KASP）技术在1 160头健康泌乳中国荷斯坦牛中进行SNP分型并进行群体遗传学分析,采用线性模型进行SNP与11个繁殖和产奶性状基于单位点和单倍型组合的关联分析。【结果】 在PIK3CB基因中共检测到了17个SNPs,筛选出7个SNPs用于后续分析。关联分析发现,7个SNPs与多个目标性状存在显著或极显著的关联（P<0.05;P<0.01）;位于外显子区域的g.130433743 A>G位点AA基因型个体和位于可变剪接区域的g.130448069 G>A位点GG基因型个体,其经产牛首末次配种间隔、产奶量、乳蛋白量和乳脂量最低,体细胞评分最高,上述基因型个体具有较短的首末次配种间隔,而产奶性能相对较差;g.130387717 G>A位点AA基因型个体,其初配日龄和青年牛首末次配种间隔最低,产奶量、乳蛋白量和乳脂量最高,该基因型个体的繁殖和产奶性能均较好,上述3个SNPs位点可作为中国荷斯坦牛繁殖和产奶性状的候选位点重点关注。单倍型分析发现,PIK3CB基因的g.130387717 G>A、g.130430832 A>－、g.130433743 A>G、g.130433982 C>T、g.130446073 C>T和g.130448069 G>A 6个SNPs紧密连锁形成一个单倍型块,且与多个目标性状存在显著或极显著关联（P<0.05;P<0.01）,其中H2H3和H2H4单倍型组合个体的繁殖和产奶性能较好,为优势单倍型组合。【结论】 中国荷斯坦牛PIK3CB基因存在丰富的遗传变异,其多态性与繁殖和产奶性状存在关联,g.130433743 A>G、g.130448069 G>A和g.130387717 G>A位点可作为潜在分子标记,为中国荷斯坦牛的平衡育种提供理论依据。 相似文献
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In order to study the influence of genetic variation in CYP2C45 gene on fatty liver production performance, PCR and sequencing methods were used to identify polymorphism of CYP2C45 gene exons in 113 Landes goose, and the association of polymorphism with production performance of goose fatty liver were investigated. The results indicated that there were 4 SNPs in exon 5 at 111 bp of C>T transition and at 147 bp of C>T transition, and in exon 7 at 71 bp of A>G transition and at 135 bp of G>A transition, respectively. These SNPs were in the stage of complete linkage by linkage disequilibrium analysis. Combining 4 SNPs as one genotype, there were AA (CCAG/CCAG) and AB (CCAG/TTGA) genotypes, but BB genotype (TTGA/TTGA) was not found， the genotype frequencies were 0.9027 and 0.0973, respectively, and the gene frequencies of alleles A and B were 0.9513 and 0.0487, respectively. Analysis of variance showed that the body weight of AB genotype was higher significantly than AA genotype (P<0.05), although the liver weight and ratio of liver weight versus body weight of AA genotype were higher than AB genotype, there were no significant difference （P>0.05）. It suggested that, in the case of the same yield of liver production, less body weight less feeding, AA genotype maybe contribute to the improvement of production efficiency. 相似文献
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为了解三穗鸭肌细胞增强因子(myocyte enhancer factor 2A,MEF2A)SNPs与屠宰性状的相关性,本研究以60只三穗鸭为研究对象,采用PCR-SSCP方法结合PCR产物直接测序技术对三穗鸭MEF2A基因多态性进行检测,并进行SNPs与屠宰性状各指标的相关分析。结果表明,在MEF2A基因中共发现了2个SNPs:第11外显子的g.47915G>A位点和g.47918G>A位点,g.47915G>A位点发生的G/A突变使密码子由GAA变为AAA,翻译出的氨基酸由谷氨酸变成赖氨酸,g.47918G>A位点的G/A突变引起的密码子由GAT变成AAT,翻译出的氨基酸由天冬氨酸变成天冬酰胺。SNPs与屠宰性状的关联性分析表明,g.47915G>A和g.47918G>A位点影响全净膛率。这一结果揭示了MEF2A基因的多态性对三穗鸭屠体性状具有重要的影响。 相似文献