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1.
Microcosm experiments were carried out to study the effects of bacterial-feeding nematodes and prometryne on soil bacterial communities in contaminated soil. Prometryne (5 or 10 mg kg−1 dry soil, that is, P5 or P10) and bacterial-feeding nematodes (5 or 10 individuals g−1 dry soil, that is, N5 or N10), singly and in combination (P5N5, P5N10, P10N5, P10N10), were added to a nematode-free soil. An uncontaminated nematode-free soil was studied for comparison (Control). Bacterial-feeding nematode grazing boosted soil enzyme activities in contaminated soils, thus speeding up prometryne degradation. In the initial stage of the experiment, prometryne enhanced the soil enzyme activities too, but served the opposite purpose later. Denaturing gradient gel electrophoresis (DGGE) analysis indicated that prometryne contamination and nematode grazing over the incubation period exerted an obvious impact on Species richness (S), Shannon–Wiener index (H′) and Evenness (EH) of soil bacteria, which increased initially, then decreased and increased again later. The cluster analysis of DGGE profiles showed that the similarity of soil bacterial communities in all treatments with indigenous microbes, P5, P5N5, P5N10, P10, P10N5, and P10N10 and the Control was 75%, 44%, 78% and 49% at Day 0, Day 8, Day 18 and Day 30, respectively. Compared to the Control, DGGE profiles displayed a varying characteristic bands pattern in all treatments over the incubation period with certain bands present in the treatments while not in the Control and vice versa, suggesting that bacterial-feeding nematode grazing and prometryne contamination affected soil bacterial communities evidently. Consequently, when added to contaminated soil, bacterial-feeding nematodes can contribute to restoration of contaminated sites by degrading toxic compounds like prometryne through enhanced microbial activity.  相似文献   

2.

Purpose  

Using two fractions of dissolved organic matter (DOM) with different polarity, we carried out the experiments with standard batch equilibration, soil column leaching, and soil thin layer chromatography to investigate the behavior of the herbicide prometryne in soils. The purpose of the study was to: (1) separate DOM into hydrophilic matter and hydrophobic acid forms and characterize their chemical properties; (2) analyze interaction between the DOM fractions and prometryne in soils.  相似文献   

3.

Purpose  

The use of only one or a few species—representing an entire taxon—in ecotoxicological standard tests poses risk of underestimating the impact of toxicants on the environment. In earthworm ecotoxicity tests, the species Eisenia fetida or Eisenia andrei are commonly used, and there is evidence that these species respond relatively insensitive towards environmental pollution. With the present study, we wanted to evaluate the risk of underestimating effects of the insecticide imidacloprid in soil organisms by comparing E. fetida with two other earthworm species (Aporrectodea caliginosa and Lumbricus terrestris) regarding their sensitivities towards soil contaminated with this widely used insecticide.  相似文献   

4.

Purpose  

This paper evaluates the feasibility of using the buffering capacity of natural soil for the remediation of dredged material before being disposed in soil landfills. To achieve that, an Integrated Soil Microcosms (ISM) system was designed to produce elutriates and leachates from the sediment/soil percentage mixtures. Furthermore, to investigate the biological effects of the contaminated sediments, the toxicity behavior of leachates and elutriates was assessed and compared by performing acute (48 h) toxicity assays with the cladoceran Daphnia magna as test organism.  相似文献   

5.

Purpose  

Successful phytoremediation depends mainly on the bioavailability of heavy metals in the soil. Recently, soil microbes possess several mechanisms that are able to change metal bioavailability in the soil, which provides a new strategy for investigating biogeochemical cycling of metals in contaminated soils. Three metal mines soils with elevated concentrations of Cd, Pb, and Zn from China were applied in this column study to (1) evaluate the effects of metal tolerant bacterial inoculation (Burkholderia cepacia, accession number: AB051408) on metal release, (2) monitor the migration of metals in the rhizospheric horizon (0–20 cm), and (3) investigate metal speciation and sequential fractions in soil.  相似文献   

6.

Purpose  

A contaminated soil was amended to reduce bioavailability of metals (As, Cd, Cu, Pb, and Zn) and to modify its potential environmental impacts. Reproduction toxicity tests using two different soil invertebrates, Enchytraeus crypticus and Folsomia candida, were used to evaluate efficiency of soil amendments to reduce metal availability.  相似文献   

7.

Purpose  

This study investigated the effects of plants on the available pools of heavy metals and their re-supply potential in contaminated substrates in a short-term experiment using five metal-accumulating willow and poplar species/cultivars and in a longer-term experiment for Salix x reichardtii.  相似文献   

8.

Purpose  

Cadmium (Cd) is a toxic heavy metal, accumulated in soil by anthropogenic activities and has serious effects on soil microbial activities in contaminated soils. Moreover, there is a lack of reliable data on the effects of Cd in the soil-plant system, since most of the information on Cd-microorganism interactions in soils are based on sewage sludge without plants. The main objective of this study was to assess the effects of Cd on soil microbial activities and community structure during growth of plant.  相似文献   

9.

Purpose  

Little is known of the effect of sepiolite on the transformation of Cd in anthropogenically contaminated paddy soil under different moisture conditions; therefore, we studied the effects of sepiolite and flooding on the extractability and fractionation of Cd in paddy soils.  相似文献   

10.

Background, aim, and scope  

In the soil environment, polycyclic aromatic hydrocarbons (PAHs) and heavy metals (HMs) are of great environmental and human health concerns due to their widespread occurrence, persistence, and carcinogenic properties. Bioremediation of contaminated soil is a cost-effective, environmentally friendly, and publicly acceptable approach to address the removal of environmental contaminants. However, bioremediation of contaminants depends on plant–microbe interactions in the rhizosphere. The microorganisms that can mineralize various PAHs have PAH dioxygenase genes like nahAc, phnAc, and pdo1. To understand the fate of pyrene in rhizospheric and non-rhizospheric soils in the presence or absence of Pb, pyrene biodegradation, bacterial community structure, and dioxygenase genes were investigated in a pot experiment.  相似文献   

11.

Purpose  

Persistent organic compounds exhibit declining extractability and bioavailability to microorganisms and other soil organisms with increasing contact time or ageing. Among the possible mechanisms are the association of organic compounds with natural organic matter. Ageing can reduce the negative effects that an organic pollutant may have on the biological, biochemical properties and phytotoxicity of the contaminated soil. The aim of the present work was to evaluate, under laboratory conditions, the response of an agricultural soil contaminated with phenanthrene (Phe) and subjected to 2-year ageing and the effects and potential remediation capabilities of compost and an effective Phe-degrading bacterial culture.  相似文献   

12.

Purpose  

Ozonation has been shown to be a feasible method for removal of organic pollutants resistant to biodegradation in contaminated soils. However, little is known about the impact of ozonation on soil organic matter (SOM). This study was conducted to investigate the change in SOM characteristics and the consequent change in sequestration of contaminants in soil during ozonation.  相似文献   

13.

Purpose  

UK local authorities are required by environmental regulations to keep a public register of contaminated land. In many towns and cities, there is growing concern of allotment holders about the quality of their allotment soils. Limited information on soil chemistry is available for the southwest of England. This work was carried out to assess whether any allotment and park soil in Bristol could be defined as “contaminated”.  相似文献   

14.

Purpose

Polycyclic aromatic hydrocarbons (PAHs) are largely accumulated in soils in China. The immobilized-microorganism technique (IMT) is a potential approach for abating soil contamination with PAHs. However, few studies about the application of IMT to contaminated soil remediation were reported. Due to recalcitrance to decomposition, biochar application to soil may enhance soil carbon sequestration, but few studies on the application of biochars to remediation of contaminated soil were reported. In this study, we illustrated enhanced bioremediation of soil having a long history of PAH contamination by IMT using plant residues and biochars as carriers.

Materials and methods

Two PAH-degrading bacteria, Pseudomonas putida and an unidentified indigenous bacterium, were selected for IMT. The extractability and biodegradation of 15 PAHs in solution and an actual PAH-contaminated soil amended with immobilized-bacteria materials were investigated under different incubation periods. The effects of carriers and the molecular weight of PAHs on bioremediation efficiency were determined to illustrate their different bio-dissipation mechanisms of PAHs in soil.

Results and discussion

The IMT can considerably enhance the removal of PAHs. Carriers impose different effects on PAH bio-dissipation by amended soil with immobilized-bacteria, which can directly degrade the carrier-associated PAHs. The removal of PAHs from soil depended on PAH molecular weight and carrier types. Enhanced bio-dissipation by IMT was much stronger for 4- and 5-ring PAHs than for 3- and 6-ring ones in soil. Only P400 biochar-immobilized bacteria enhanced bio-dissipation of all PAHs in contaminated soil after a 90-day incubation.

Conclusions

Biochar can promote bioremediation of contaminated soil as microbial carriers of IMT. It is vital to select an appropriate biochar as an immobilized carrier to stimulate biodegradation. It is feasible to use adsorption carriers with high sorptive capabilities to concentrate PAHs as well as microorganisms and thereby enhance dissipation of PAHs and mitigate soil pollution.  相似文献   

15.
The population abundance of free-living and plant-parasitic nematodes was investigated in a long-term rotation/tillage/stubble management experiment at Wagga Wagga Agricultural Institute, New South Wales (NSW), Australia. The treatments were a combination of two crop rotations: wheat (Triticum aestivum)–wheat and wheat–lupin (Lupinus angustifolius); two tillage systems: conventional cultivation (CC) and direct drill (DD); and two stubble management practices: stubble retention (SR) and stubble burnt (SB). Plots of one of the wheat–wheat treatments received urea at 100 kg N ha−1 during the cropping season. Soil samples from 0–5 and 5–10 cm depths were collected in September (maximum tillering), October (flowering) and December (after harvest), 2001, to analyse nematode abundance. Soil collected in September was also analysed for concentrations of total and labile C, and pH levels.Three nematode trophic groups, namely bacteria-feeders (primarily Rhabditidae), omnivores (primarily Dorylaimidae excluding plant-parasites and predators) and plant-parasites (Pratylenchus spp. and Paratylenchus spp.) were recorded in each soil sample. Of them, bacteria-feeders (53–99%, population range 933–2750 kg−1 soil) dominated in all soil samples. There was no difference in nematode abundance and community composition between the 0–5 cm and 5–10 cm layers of soil. The mean population of free-living and plant-parasitic nematodes varied significantly between the treatments in all sampling months. In most cases, total free-living nematode densities (Rhabditidae and Dorylaimidae) were significantly (P < 0.001) greater in wheat–lupin rotation than the wheat–wheat rotation irrespective of tillage and stubble management practices. In contrast, a greater population of plant-parasitic nematodes was recorded from plots with wheat–wheat than the wheat–lupin rotation. For treatments with wheat–wheat, total plant-parasitic nematode (Pratylenchus spp. and Paratylenchus spp.) densities were greater in plots without N-fertiliser (295–741 kg−1 soil) than the plots with N-fertiliser (14–158 kg−1 soil).Tillage practices had significant (P < 0.05) effects mostly on the population densities of plant-parasitic nematodes while stubble management had significant effects (P < 0.05) on free-living nematodes. However, interaction effects of tillage and stubble were significant (P < 0.01) for the population densities of free-living nematodes only. Population of Rhabditidae was significantly higher in conventional cultivated plots (7244 kg−1 soil) than the direct drilled (3981 kg−1 soil) plots under stubble retention. In contrast, plots with direct drill and stubble burnt had significantly higher populations of Dorylaimidae than the conventional cultivation with similar stubble management practice. No correlations between abundance of free-living nematodes, and concentration of total C and labile C in soil were observed in this study. These results showed that stubble retention contributed for enormous population density of free-living (beneficial) nematodes while conventional cultivation, irrespective of stubble management, contributed for suppressing plant-parasitic nematodes.  相似文献   

16.

Purpose  

Gardening (especially food growing) in urban areas is becoming popular, but urban soils are often very contaminated for historical reasons. There is lack of sufficient information as to the bioavailability of soil heavy metals to plants and human in urban environments. This study examines the relative leachability of Cr, Ni, As, Cd, Zn, and Pb for soils with varying characteristics. The speciation and mobility of these metals can be qualitatively inferred from the leaching experiments. The goal is to use the data to shed some light on their bioavailability to plant and human, as well as the basis for soil remediation.  相似文献   

17.

Purpose  

The purpose of the present study was to investigate the composition and structure of microbial communities in rhizosphere soils in response to the presence of Aroclor 1242 with low (8 mg kg−1 soil) and high (16 mg kg−1 soil) concentrations in the hope to provide more information on potential dissipation of polychlorinated biphenyls (PCBs) at contaminated sites.  相似文献   

18.

Purpose  

Dissimilatory iron-reducing bacteria have been described by both culture-dependent and -independent methods in various environments, including freshwater, marine sediments, natural wetlands, and contaminated aquifers. However, little is known about iron-reducing microbial communities in paddy soils. The goal of this study was to characterize iron-reducing microbial communities in paddy soil. Moreover, the effect of dissolved and solid-phase iron (III) species on the iron-reducing microbial communities was also investigated by enrichment cultures.  相似文献   

19.

Purpose  

Two main pathways of phytoremediation of heavy metal-contaminated soils are phytostabilization and phytoextraction. Some soil amendments can strengthen phytostabilization or phytoextraction through either reducing heavy metal bioavailability in soil or increasing the heavy metal accumulation capacity of the hyperaccumulator (enhancing heavy metal concentration or shoot biomass of the hyperaccumulator). Urea and chicken manure are often used as fertilizers. This research will explore their effects on a newly found hyperaccumulator, Rorippa globosa (Turcz.) Thell., phytoremediating cadmium (Cd).  相似文献   

20.

Purpose  

We investigated the chemical fractions of Zn, Cd and Cu in soils collected from positions at different distances from a copper smelter and studied the relationships between distribution patterns of Zn, Cd and Cu, fractions and soil organic carbon (SOC), especially “black carbon” (BC), in contaminated soils. The relationships between soil particle size and concentrations of Zn and Cd in contaminated soil were also examined.  相似文献   

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