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广西洞穴盲鱼1新属3新种 总被引:1,自引:0,他引:1
本文记述了采集于广西河池地区的洞穴盲鱼1新属3新种,即原花鳅属Protocobitis gen.nov.;无眼原花鳅Protocobitis typhlops sp.nov.、透明间条鳅Heminogmacheilus hyalinus sp.nov.、叉背金线鲃Sinocyclocheilus furcodorsalis sp.nov.。3新种均分布于石山区的地下溶洞,营穴居生活,眼睛均已完全退化,无色素,体白色半透明。 相似文献
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将7日龄艾维因肉仔鸡分成3组,每组接种相同剂量鸡肾型支病毒强毒病料。同日,Ⅰ组采用0. 3%NaHCO3饮水治疗,Ⅱ组采用0. 5%肾炎康拌料治疗,Ⅲ组作为对照,连续治疗12d。统计结果,Ⅰ组、Ⅱ组、Ⅲ组死于肾型传支数量分别为0只、2只、5只,其死亡率分为0、20%、62. 5%。经t检验,Ⅲ组与Ⅰ组间死亡率差异极显著(t=2. 9427),Ⅲ组与Ⅱ组间死亡率差异不显著(t=1. 8379),Ⅱ组与Ⅰ组间死亡率差异不显著。对比治疗结果表明,碳酸氢钠用于治疗鸡肾型传支疗效显著而确实,且药费低廉。 相似文献
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In this paper, genetic diversity of intraspecies, and genetic relationship of interspecies in Epinephelus spp. (E. merra, E. fario, E. awoara, E. akaara and E. septemfasciatus) were assessed by using mitochondrial DNA restriction fragment length polymorphisms (mtDNA RFLPs).The samples were collected from the coastal area of Zhanjiang,Guangdong province. MtDNA was extracted from the fresh liver tissue by applying a difference centrifugation procedures. Using 17 restriction enzymes with 5-or 6-bp recognition sites, the purified mtDNA was cleaved by single enzymes. These enzymes included BamH Ⅰ,Bgl Ⅰ,Bgl Ⅱ,Dra Ⅰ,EcoR Ⅰ,EcoR Ⅴ,Hind Ⅲ,Kpn Ⅰ,Mlu Ⅰ,Pst Ⅰ,Pvu Ⅱ,Sal Ⅰ,Sca Ⅰ,Sma Ⅰ,Sty Ⅰ,Xba Ⅰ and Xho Ⅰ. The phylogenetic analysis was done using the Neighbor joining(NJ) method and Unweighted pairgroup method with arithmetic mean(UPGMA) method. Genetic diversity indices such as haplotype diversity (h), average genetic distance between haplotypes (P) and nucleotide diversity (π) were calculated using Nei and Li's segment method to quantify the genetic diversity within species. There were 8, 5, 8, 5 and 2 haplotypes detected within E. merra, E. fario, E.awoara, E.akaara and E. septemfasciatus, respectively. The haplotype diversity (h) was 0.8943, 0.6186, 0.9242, 0.6927 and 0.1820,respectively. The average genetic distance between haplotypes (P) was 0.62%±0.31%, 0.64%±0.37%, 1.12%±0.55%,0.72%±0.42% and 0.45%, respectively. And the nucleotide diversity (π) was 0.22%, 0.13%, 0.46%, 0.17% and 0.04%, respectively. The wild groupers in the Zhanjiang Coastal Area exhibited a relative higher level of genetic diversity. The net genetic distance between species (Pnet) was 0.0694(E. merra - E. fario),0.1337(E. merra - E. awoara),0.1090 E. merra -E. akaara), 0.1286(E. merra - E. septemfasciatus),0.1590(E. fario -E. awoara),0.0825(E. fario -E. akaara),0.1153(E. fario - E. septemfasciatus),0.1131 E. awoara - E. akaara),0.0724(E. awoara -E. septemfasciatus) and 0.1336(E. akaara - E. septemfasciatus). Both NJ and UPGMA methods yielded an identical phylogenetic tree for the five species. The E. merra and E. fario first clustered together, then joined with E. akaara, and finally clustered with E. awoara and E. septemfasciatus. 相似文献
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一、三文鱼发病前一般有如下征兆1.离群鱼或游于水面、排水部的鱼或在池壁、池底缓游的鱼增多。2.游泳方式异常,出现突进式、旋回式、竖直上下式、曲体或侧体游动或擦磨活动。3.活泼度、集群性下降,摄饵反应迟钝,摄饵量减少。4.体色发暗、变黑或呈灰黄色。5.体表局部浮肿或充血,出现白点或白色粘液。6.鱼鳍缺损,基部充血或颜色变白。二、常见疾病及其防治(一)传染性胰脏坏死症症状:鳃色淡、贫血,前腹部膨胀,消化道无饵,含透明白色粘液,肝脏白色或充血,幽门垂出现凝血块。主要危害开食2~4周稚鱼。防治:因病毒深入卵内部,尚无有… 相似文献
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一、准备工作 1.池塘的选择:大小不限,以 0.5~ 3亩为宜,深 1.0米以上,底质最好是壤土,保水保肥效果好;水源充足,水质良好无污染,进排水方便;池埂牢固,池塘光照要好,有利于培养饵料生物。 2.清塘:鱼苗下塘前 20天左右排干水,清除过多淤泥,平整池底并曝晒数日,然后用生石灰彻底清塘,每亩用量为 100~ 125公斤。 3.肥水:清塘后 2~ 3天,即鱼苗下塘前 5~ 7天,加水至 50厘米深,然后施经发酵的有机粪肥,培养水中的浮游生物,使鱼苗下塘后,能吃到适口的天然饵料,施肥量为每亩 100~ 200公斤。 4.放苗前的准… 相似文献
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应用红细胞C3b受体花环试验和红细胞天然免疫粘附肿瘤细胞花环试验方法,证实兴国红鲤(Cyprinuscarpio.var.singguonensis)、玻璃红鲤(C.carpio.var.wananensis)、荷包红鲤(C.carpio.var.wananensis)及瓯江彩鲤(C.carpio.var.color)的红细胞表面都存在C3b补体受体,均可形成花环;红鲤的红细胞免疫粘附能力存在着种群间差异,上述2种花环试验的花环率大小的顺序均是:兴国红鲤>荷包红鲤>瓯江彩鲤>玻璃红鲤,差异都极显著(P<0.01);4群体红鲤的红细胞均具有吞噬作用。结果证实,鱼类红细胞免疫功能在机体防御病原的过程中占有重要地位。 相似文献
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中华鳑鲏学名Rhodeus sinensis ,俗称鳑鲏,鲤科,体侧扁,略呈椭圆形。体长约4-15cm.头小,眼大,口端位,麟圆,侧线不完全。 相似文献
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鱼类体表寄生虫病的防治方法 总被引:1,自引:0,他引:1
一、预防方法1.放养前清除池底淤泥,彻底消毒池塘,杀灭媒介生物等。2.严格执行检疫制度,不从疫情地区引进苗种等。3.保持合理的密度。4.加强水质管理,投喂营养丰富饵料,提高鱼体抵抗力。5.养殖工具(网具、木桶、木瓢等)、食场等经常洗涤、消毒。定期检查,发现病害后及时治疗并严格隔离病源。6.利用病原体对寄主具有选择性,采用轮养方法。二、寄生虫病的防治方法1.小瓜虫①200~250毫克/升冰醋酸溶液浸洗15分钟或2毫克/升醋酸亚汞或硝酸亚汞溶液浸洗1.5~3小时或0.2~0.4毫克/升孔雀石绿溶液浸洗2小时。②0.1~0.3毫克/升的醋酸亚汞或… 相似文献