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1.
IGF-Ⅰ和EGF对绵羊毛囊体外培养的影响 总被引：2，自引：0，他引：2
在常规培养液中分别添加不同剂量的细胞生长因子IGFⅠ(100 ng.mL-1、10 ng.mL-1、1 ng.mL-1、0.1ng.mL-1)和EGF(100 ng.mL-1、20 ng.mL-1、2 ng.mL-1、0.2 ng.mL-1),观察毛囊体外培养过程中生长速度及毛囊形态结构的变化。结果表明,10 ng.mL-1IGFⅠ和20 ng.mL-1EGF对毛囊均具有明显的促生长作用,10 ng.mL-1IGFⅠ有刺激毛囊生长正向调节作用,20 ng.mL-1EGF可促进毛囊外根鞘细胞分化,并诱导毛囊由生长期提前进入退行期。10 ng.mL-1IGFⅠ和20 ng.mL-1EGF混合对毛囊生长有明显的促进作用。 相似文献
2.
镍对蚕豆根尖细胞的遗传毒性效应 总被引：2，自引：1，他引：1
采用蚕豆根尖细胞微核试验,研究了不同浓度硝酸镍(5～5 1300 μmol·L-1)对蚕豆根尖生长、细胞有丝分裂指数(MI)、微核率以及染色体畸变的影响,以此评价镍的遗传毒性和蚕豆根尖微核技术应用于镍污染检测的可行性.结果表明,5 μmol·L-1镍显著促进根的生长,提高有丝分裂指数,对微核形成和染色体畸变无明显影响.在10～5 000 μmol·L-1镍浓度范围,蚕豆根的生长受阻,有丝分裂指数下降,表现出明显的剂量-效应关系;微核率和染色体畸变率先升高,且呈明显的剂量-效应关系,分别于100 μmol·L-1和50 μmol·L-1达到最大值;随着镍浓度的增加,两者均下降,但仍显著高于对照.镍引起的染色体畸变以染色体断片和落后染色体为主.这些结果表明,镍能诱导蚕豆根尖细胞微核的产生和染色体畸变,具有一定的遗传毒性;同时表明,在5～500 μmol·L-1浓度范围内,蚕豆根尖微核检测体系可应用于镍污染的监测. 相似文献
3.
《扬州大学学报(农业与生命科学版)》2017,(2)
通过小鼠鞘内注射地佐环平(MK801,3、10nmol·L-1)和美金刚(10、30nmol·L-1),运用免疫组织化学染色的方法,探讨这2种药物是否对脊髓神经元产生促进凋亡的作用。结果表明:小鼠鞘内注射MK801和美金刚对脊髓背角神经元(主要为感觉神经元)、腹角神经元(主要为运动神经元)及其他细胞均无促凋亡作用,且对腰段脊髓横切面上的细胞数量无显著影响。该试验为研究NMDA受体在脊髓层面作为用药靶点的安全用药提供了基础和前提。 相似文献
4.
为探究雄激素受体(Androgen receptor,AR)及共调节因子对绵羊附睾上皮细胞(Epididymal epithelial cells,EECs)谷胱甘肽过氧化物酶5(Glutathione peroxidase 5,GPX5)的调节机制,本研究采用CCK-8检测睾酮对EECs增殖的影响;利用qRT-PCR、免疫荧光法和 Western Blot法分别检测AR、GPX5、甾体激素受体共激活子 1(Steroid receptor coactivator 1,SRC-1)、CREB结合蛋白(cAMP-response element-binding protein,CBP)、p300和核受体共抑制因子2(Nuclear receptor corepressor 2,NCOR2)的mRNA水平和蛋白表达情况,采用双荧光素酶报告基因测定干扰SRC-1或p300后EECs的荧光素酶活性。结果表明:1)与对照组相比,100 nmol/L睾酮组细胞中的GPX5 mRNA和蛋白表达量极显著升高(P<0.01),1 000 nmol/L睾酮组细胞中的GPX5 mRNA和蛋白表达量差异不显著(P>0.05),但分别极显著(P<0.01)和显著(P<0.05)低于100 nmol/L睾酮组;100 nmol/L睾酮组细胞中的AR mRNA和蛋白表达量分别呈极显著(P<0.01)和显著(P<0.05)高于对照组,然而1 000 nmol/L睾酮组细胞中的AR mRNA和蛋白的表达量显著低于对照组(P<0.05);1 000 nmol/L睾酮组细胞中的CBP、p300、SRC-1蛋白表达极显著高于对照组(P<0.01),特别是核内的表达量明显增高。2)与对照组相比,siRNA-AR组细胞中的GPX5 mRNA和蛋白的表达量差异不显著(P>0.05),而SRC-1和p300蛋白表达量极显著升高(P<0.01)。pcDNA3.1-AR组GPX5 mRNA和蛋白表达量较对照组呈极显著(P<0.01)和显著(P<0.05)升高。3)siRNA-SRC-1 和siRNA-p300组细胞中的GPX5 mRNA和蛋白表达量较对照组均显著降低(P<0.05),而AR的蛋白表达量与对照组差异不显著(P>0.05),AR的转录活性显著降低(P<0.05)。综上,睾酮对绵羊EECs GPX5、AR及其共调节因子的表达具有浓度依赖性的调节效应,睾酮通过AR及其共调节因子SRC-1和p300/CBP的协同调节GPX5表达。本研究为探究GPX5在绵羊附睾中的调节机制提供理论依据。 相似文献
5.
用全骨髓法培养大鼠骨髓细胞,用第3代细胞进行试验,研究杜仲与地塞米松共作用对骨髓基质细胞增殖和分化的影响.试验分为对照组、地塞米松作用组(浓度分别为1、10、100、1000nmol/L)、杜仲与地塞米松共作用组(分别在地塞米松作用组中加入杜仲,使杜仲浓度为40μg/mL).作用6d后,MTT法测定细胞增殖活力并测定培养基中碱性磷酸酶活性;作用14d后,油红O染色法测定细胞内脂肪滴.结果表明,1～100nmol/L地塞米松对细胞的增殖无明显影响,1nmol/L地塞米松能提高碱性磷酸酶的活性,1000nmol/L地塞米松能显著抑制细胞的增殖和碱性磷酸酶的活性,杜仲与1000nmol/L地塞米松共作用可显著改善由单独1000nmoL/L地塞米松所致的抑制作用;1～100nmol/L地塞米松作用组细胞肉无明显的脂肪滴形成,1000nmol/L地塞米松作用组细胞内出现脂肪滴,杜仲与1000nmol/L地塞米松共作用组细胞内无明显脂肪滴.表明杜仲能于预高浓度(1000nmol/L)塞米松对骨髓基质细胞增殖的抑制作用,并能抑制地塞米松促进骨髓基质细胞向脂肪细胞方向的分化. 相似文献
6.
云南茶叶茸毒蛾的生物学特性与综合防治 总被引：1，自引：0，他引：1
对云南茶叶上发生危害的茸毒蛾主要种类的生物学特性及综合防治研究进行了报道.云南茶园内常见的6个茸毒蛾种类,以茶茸毒蛾、皱茸毒蛾和蔚茸毒蛾危害为主.茶茸毒蛾在茶园中1年发生4～5个世代;生长时期完成1个世代需要61.2～71.5d;越冬代长达146.8d.茸毒蛾的综合防治,充分利用茶叶——害虫——天敌相互作用的生态调控作用,发展生物制剂、农业防治、生态防治等结合的综合治理方法,尽力减少化学农药使用量或者不用化学农药,建立可持继发展的害虫综合防治体系. 相似文献
7.
我国部分引进猪种IGF2和RYR1基因主效位点的遗传变异分析 总被引：1，自引：0，他引：1
胰岛素样生长因子2(Insulin-like growth factor2,IGF2)是一类多功能细胞增殖调控因子。它在胎儿生长发育、肿瘤细胞增殖、肌肉生长等方面具有重要的调控作用。猪IGF2基因第3内含子3072G>A突变是迄今少数几个被确证的影响农业动物数量性状的主效突变位点(Quantitative Traits Nucleotide,QTN)。兰定尼受体基因(RYR1)c.1843C>T突变位点是造成猪应激综合症的主效基因位点。本研究采用PCR-RFLP及PCR-SSCP的方法,检测了深圳、江苏、江西等4个猪场5个引进猪种(品系)的323头种猪个体在IGF2的第3内含子3072G>A位点突变和RYR1基因的c.1843C>T突变位点的基因型,以此指导猪的育种实践,提高猪产肉量和经济效益,剔除氟烷应激敏感基因,选育抗应激种群。 相似文献
8.
通过外科手术连续收集活体公兔外周血样 ,用放射免疫法测定血清促卵泡素 ( FSH)、促黄体生成素 ( LH)和睾酮 ( T)水平。 5只健康成年公兔分别安置颈静脉导管 ,以 2 0 min间隔连续采样 1 0次。结果表明 ,血清 FSH、LH和 T含量分别为 ( 2 4 4.95± 4 5.4 9) IU/ L、( 443.64± 1 88.4 4) IU/ L和 ( 1 32 .4 6± 99.2 4 ) nmol/L;LH和 T均呈波动式释放 ,而 FSH的波动性不明显 相似文献
9.
采用生物信息学的方法分析已经在GenBank上注册十字花科植物的DFR基因序列及相应氨基酸序列,并对其理化性质、结构特征、系统进化关系等进行预测。结果表明,十字花科植物的DFR蛋白大部分都有6个外显子,分子质量36 481.89～43 129.21 Da,大多数蛋白亚细胞定位于叶绿体中;除了BnaDFRA402,其他的DFR蛋白均具有酶活性部位、NADP结合位点和底物特异结合位点,这些保守区域形成了5个motifs;每个DFR蛋白均有多个磷酸化位点,以Ser为主,以Thr和Tyr磷酸化为辅;二级结构均由α-螺旋、β-转角、延伸链和无规则卷曲组成,其中α-螺旋为主要结构元件。 相似文献
10.
构建东北梅花鹿galectin-1基因特异小分子干扰RNA(shRNAs)真核干扰载体,并获得稳定感染生茸区骨膜细胞系。设计2对galectin-1 mRNA特异性shRNAs,两端分别带有ClaⅠ/MluⅠ酶切位点和一个9 nt发夹结构,通过基因克隆技术将其插入真核表达质粒pLVTHM中,构建galectin-1 shRNA表达载体,利用PCR和测序筛选出阳性质粒。将重组质粒与PPAX及pMD2.G共转染到293 t细胞中,在倒置显微镜下观察转染效果并收集、浓缩病毒质粒,感染生茸区骨膜细胞。结果表明,成功构建东北梅花鹿galectin-1基因RNAi载体,获得稳定传代被感染生茸区骨膜细胞系,为进一步研究galectin-1基因在鹿茸再生中的作用奠定基础。 相似文献
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Mitogens and oncogenes can block the induction of specific voltage-gated ion channels 总被引：16，自引：0，他引：16
The mechanisms underlying the ontogeny of voltage-gated ion channels in muscle are unknown. Whether expression of voltage-gated channels is dependent on mitogen withdrawal and growth arrest, as is generally true for the induction of muscle-specific gene products, was investigated in the BC3H1 muscle cell line by patch-clamp techniques. Differentiated BC3H1 myocytes expressed functional Ca2+ and Na+ channels that correspond to those found in T tubules of skeletal muscle. However, Ca2+ and Na+ channels were first detected after about 5 days of mitogen withdrawal. In order to test whether cellular oncogenes, as surrogates for exogenous growth factors, could prevent the expression of ion channels whose induction was contingent on mitogen withdrawal, BC3H1 cells were modified by stable transfection with oncogene expression vectors. Expression vectors containing v-erbB, or c-myc under the control of the SV40 promoter, delayed but did not prevent the appearance of functional Ca2+ and Na+ channels. In contrast, transfection with a Val12 c-H-ras vector, or cotransfection of c-myc together with v-erbB, suppressed the formation of functional Ca2+ and Na+ channels for greater than or equal to 4 weeks. Potassium channels were affected neither by mitogenic medium nor by transfected oncogenes. Thus, the selective effects of certain oncogenes on ion channel induction corresponded to the suppressive effects of mitogenic medium. 相似文献
13.
为研究雄激素和氢化可的松对山羊附睾上皮细胞生长的作用模式,本研究利用酶标仪检测附睾头部上皮细胞增殖情况,实时荧光定量PCR及ELISA检测雄激素受体(AR)的表达,检测睾酮与氢化可的松对山羊附睾上皮细胞体外增殖的作用以及对AR表达的影响。结果表明:100nmol/L睾酮对附睾头上皮细胞增殖的促进效应最高且与对照组差异极显著(P0.01),200nmol/L氢化可的松促进附睾头上皮细胞增殖效应最佳并与对照组差异显著(P0.05),前者的效应明显且可以被AR阻断剂阻断;睾酮和氢化可的松对附睾头上皮细胞增殖表现为协同作用;100nmol/L睾酮与200nmol/L氢化可的松均使附睾头上皮细胞的AR mRNA和蛋白表达量上调,与对照组差异显著(P0.05),且二者共存时附睾头上皮细胞的AR mRNA和蛋白表达量极显著高于对照组(P0.01)。本研究表明睾酮和氢化可的松对附睾头上皮细胞体外增殖均有促进作用,呈明显的浓度依赖性,且二者之间存在协同作用,这为进一步研究附睾上皮细胞增殖及功能的调节机理提供了基础。 相似文献
14.
HIV-1 production from infected peripheral blood T cells after HTLV-I induced mitogenic stimulation 总被引：17，自引：0，他引：17
The human immunodeficiency virus type 1 (HIV-1) and human T-cell leukemia virus type I (HTLV-I) are two distinct human retroviruses that infect T cells. Recent epidemiologic studies have identified a cohort of individuals that are coinfected with both viruses. It is reported here that human peripheral blood leukocytes infected with HIV-1 in vitro can be induced to produce large quantities of HIV-1 after mitogenic stimulation by noninfectious HTLV-I virions. It is also shown that HTLV-I virions may exert this effect prior to, immediately following, or well after the cells are infected with HIV-1. These results provide further impetus for epidemiologic studies of dually infected individuals to determine whether HTLV-I may act as a cofactor for acquired immunodeficiency syndrome (AIDS). 相似文献
15.
Growth regulation of human melanocytes: mitogenic factors in extracts of melanoma, astrocytoma, and fibroblast cell lines 总被引：5，自引：0，他引：5
Melanocytes derived from fetal or adult skin do not propagate in vitro unless cultured in the presence of factors such as 12-O-tetradecanoylphorbol 13-acetate (TPA). In a search for physiological factors regulating the growth of melanocytes, extracts of various cultured cell types were tested. Factors produced by melanoma and astrocytoma cell lines support continued proliferation of melanocytes in the absence of TPA. WI-38, a fibroblast cell line derived from human embryonic lung, was the most active source of melanocyte growth factors. No melanocyte growth-promoting activity was found in extracts of cultured neuroblastoma, renal cancer, normal keratinocytes, or renal epithelium. Nerve growth factor, epidermal growth factor, melanocyte-stimulating hormone, transforming growth factor-beta, and platelet-derived growth factor did not have growth-promoting activity for melanocytes. The presence of melanocyte growth factors and TPA together resulted in the strongest mitogenic activity for melanocytes, permitting the recovery (at 20 days) of 4 to 20 times as many cells as in growth factor or TPA alone. 相似文献
16.
E A Bauer N Silverman D F Busiek A Kronberger T F Deuel 《Science (New York, N.Y.)》1986,234(4781):1240-1243
Patients with Werner's syndrome, an autosomal recessive disorder, undergo an accelerated aging process that leads to premature death. Fibroblasts from such patients typically grow poorly in culture. Here it is shown that fibroblasts from a patient with Werner's syndrome have a markedly attenuated mitogenic response to platelet-derived growth factor (PDGF) and fibroblast growth factor (FGF). In contrast, they have a full mitogenic response to fetal bovine serum. Both PDGF binding and receptor numbers per cell are unaltered. The Werner's syndrome cells express high constitutive levels of collagenase in vitro. Although PDGF enhances collagenase expression through increased levels of hybridizable collagenase messenger RNA in normal skin fibroblasts, no induction of collagenase occurs in the Werner's syndrome fibroblasts. Moreover, the failure to respond to this agonist effect of PDGF is not restored by fetal bovine serum. The data suggest that failure of one or more PDGF-mediated pathways in Werner's syndrome cells may contribute to the phenotypic expression of the disorder. 相似文献
17.
Androgen accumulation andbinding to macromolecules in seminal vesicles: inhibition cyproterone 总被引：4，自引：0，他引：4
Cyproterone reduces the accumulation of testosterone and dihydrotestosterone in seminal vesicles 30 minutes after intravenous administration of tritiated testosterone to castrated rats. Testosterone, added in vitro, binds to macromolecules from the supernatant fraction of the seminal vesicle homogenates; this interaction is antagonized competitively by cyproterone. Cyproterone may diminish androgenic effects by competition for binding molecules. 相似文献
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利用山羊睾丸间质细胞体外培养的方法,研究ＩＧＦ－Ⅰ对ｃＡＭＰ和睾酮的影响。结果表明：①ＩＧＦ－Ⅰ可使间质细胞外ｃＡＭＰ积聚能力的显著增强（Ｐ＜０．０５～Ｐ＜０．００５）。②ＩＧＦ－Ⅰ可使间质细胞分泌睾酮的能力显著增加（Ｐ＜０．０５～Ｐ＜０．０１）。③ＩＧＦ－Ⅰ可刺激山羊间质细胞经ｈＣＧ诱导睾酮分泌的显著增多（Ｐ＜０．０５～Ｐ＜０．０１）。以上结果与催乳素和白细胞介素－ｌα这些含氮激素（因子）调节睾酮分泌的机理不同。 相似文献
20.
Pertussis toxin-sensitive pathway in the stimulation of c-myc expression and DNA synthesis by bombesin 总被引：20，自引：0，他引：20
The bombesin-like peptides are potent mitogens for Swiss 3T3 fibroblasts, human bronchial epithelial cells, and cells isolated from small cell carcinoma of the lung. The mechanism of signal transduction in the proliferative response to bombesin was investigated by studying the effect of Bordetella pertussis toxin on bombesin-stimulated mitogenesis. At nanomolar concentrations, bombesin increased levels of c-myc messenger RNA and stimulated DNA synthesis in Swiss 3T3 cells. Treatment of the cells with pertussis toxin (5 nanograms per milliliter) completely blocked bombesin-enhanced c-myc expression and eliminated bombesin-stimulated DNA synthesis. This treatment had essentially no effect on the mitogenic responses to either platelet-derived growth factor or phorbol 12,13-dibutyrate. These results suggest that the mitogenic actions of bombesin-like growth factors are mediated through a pertussis toxin-sensitive guanine nucleotide-binding protein. Furthermore they indicate that bombesin-like growth factors act through pathways that are different from those activated by platelet-derived growth factor. 相似文献