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1.
Vegetative propagation techniques are recognized as indispensable tools for mass multiplication of important multipurpose
trees adopted in different agroforestry systems. Albizia procera, one among important species, is difficult to propagate commercially either by stem / root cuttings or layering. A study
was undertaken to develop procedure for its in vitro regeneration through organogenesis. Explants collected from 15±2 yr-old mature plus trees and from 15 days old juvenile seedlings
were regenerated with exogenous application of different hormones. Epicotyl and hypocotyl explants excised from juvenile seedlings
showed higher callusing than axillary bud and shoot tip explants derived from mature trees. Benzylaminopurine (BA) at 3 μg/l
was most effective, which induced hundred percent callusing in epicotyl and hypocotyl explants in 1/2 Murashige and Skoog
(MS) medium. Callus originated from axillary buds and apical shoot tips of mature trees failed to form organs, however callus
derived from epicotyl and hypocotyl explants proliferated and formed de novo shoots and leaflets. A concentration of 3 μg/l of BA was found effective for shoot proliferation. Shoots grew vigorously
in 2 μg/l gibberellic acid (GA3) treatment and rooted in 1/2 MS medium supplemented with indole-3-butyric acid (IBA) and indole-3-acetic acid (IAA). Rooting
was most successful on medium supplemented with 6 μg/l IBA alone on which 93.3% of the shoots formed roots. Sand or vermiculite
supplemented with 4 ml of yoshida solution proved as best hardening media, which recorded 70-80% survival of plantlets. One
year old tissue culture raised plants had comparatively more height, collar diameter, biomass, and root shoot ratio than plants
raised from cuttings and seeds of the same age. The procedures enumerated provide a basis for the development of in vitro techniques for rapid multiplication of A. procera.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
2.
Growth and branching habit of rooted cuttings collected from epicormic shoots of Betula pendula Roth
Patterns of shoot growth and branching were studied over two growing seasons in rooted cuttings collected from both epicormic shoots and seedlings of Betula pendula Roth. Epicormic shoots were induced to sprout on stumps and small logs of 5-, 10- and 30-year-old trees. The use of epicormic shoots enhanced the rooting capacity of stem cuttings collected from these shoots but did not appear to reverse the process of maturation. In this study, maturation was based on characteristics typical of mature trees but not necessarily those of the mother plant, because it was not possible to root cuttings, for comparison, from 5-, 10- and 30-year-old ortets, other than from epicormic shoots. There was evidence of the persistence of mature characteristics through an increase in shoot plagiotropism with increasing ortet age. Rooted cuttings from both seedlings and epicormic shoots, however, assumed an increasingly orthotropic habit with a smaller shoot angle at the end of the first growing season than at the beginning and this continued into the second growing season. Other indications of maturation, such as delayed bud flushing and the incidence of flowering with increasing ortet age, were also evident in rooted cuttings from epicormic shoots. There was a clear difference in branching habit depending on cutting source. Rooted cuttings derived from epicormic shoots produced nearly twice as many lateral branches compared with rooted cuttings collected from seedlings, but this was not an effect of maturation. There was some evidence that rooted cuttings derived from seedlings grew taller than rooted cuttings from epicormic shoots. 相似文献
3.
Seedlings, rooted cuttings from juvenile stock plants, and cotyledon-derived tissue culture plantlets were propagated from several coastal Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) full-sib families so that the rooted cuttings and plantlets were clonally identical. The stock types (seedlings, rooted cuttings and plantlets) were planted in the field in spring 1987. In fall 1991, after five complete growing seasons, the plants were measured and these values compared to maturation "markers" identified for Douglas-fir in the companion paper (Ritchie and Keeley 1994). Nodal branch lengths and nodal branch diameters decreased in the order seedlings > rooted cuttings > plantlets. The decreases were about 21% for nodal branch lengths and 24% for nodal branch diameters. Seedlings carried significantly more total branches (nodal + internodal) than the other two stock types. Height growth was similar for the three stock types, but plantlet height increment was beginning to decrease during the fourth year. We conclude that vegetative propagules of Douglas-fir exhibited traits of mature trees. These were particularly marked in the cotyledon-derived plantlets. 相似文献
4.
Tests were conducted to assess rootability of red alder softwood cuttings from shoots of young trees and epicormic sprouts of mature individuals. Ortets were 3 to 34 years old, and treatments with and without indole-3-butyric acid (IBA) were compared. The extent of rooting and root vigor on the cuttings varied greatly among ortets and treatments. Among nonterminal cuttings, the best overall rooting success, with and without IBA, was obtained with cuttings of the youngest ortet. Average rooting of terminal and nonterminal cuttings of one clone were 100% and 64%, respectively. Although cuttings of some trees rooted well without IBA, the IBA treatment appeared to be generally beneficial. For all nonterminal cuttings of all clones, best results (80.0%/x) were obtained with a 10-sec dip in 2,000 or 4,000 ppm IBA. Vigorous planting stock was obtained from the rooted cuttings of all clones. Using epicormic sprouts from mature trees of proven performance will presumably allow propagation of superior trees and establishment of new plantations with improved stock. 相似文献
5.
Despite many studies of the percent loss of hydraulic conductivity in excised branches, there is doubt as to whether cutting stems in air introduces unnatural embolism into the xylem at the cut surface. To address this question, hydraulic conductivity was measured in seedlings of northern red oak (Quercus rubra L.) and rooted scions of eastern cottonwood (Populus deltoides Bartr. ex Marsh.) that had been droughted in pots. Results indicate that in situ dehydration produced a very similar vulnerability curve (% loss of conductivity versus water potential) to those previously obtained by bench-top dehydration of excised branches of eastern cottonwood and red oak. In eastern cottonwood cuttings, conductivity loss increased sharply below water potentials of -1.0 MPa, with 100% loss of conductivity occurring by -2.0 MPa, whereas conductivity loss in red oak seedlings was more gradual, i.e., increasing below -1.5 MPa and sustaining 100% loss of conductivity by about -4.0 MPa. 相似文献
6.
To determine whether brassinosteroids promote clonal propagation of mature coniferous trees, cuttings of adult Norway spruce (Picea abies (L.) Karst.) trees were harvested in late March, treated with (22S,23S)-28-homobrassinolide (SSHB), and stored at 2 degrees C in darkness until they were planted in May. Treatment of the cuttings with SSHB significantly enhanced the formation of adventitious roots by increasing percent rooting from 50% in the control cuttings to 92% in the 60 ppm SSHB treatment. N(6)-Benzyladenine (BA) stimulated rooting slightly although not significantly, but it counteracted the stimulative effect of SSHB when cuttings were treated with both compounds together. In a second study, the above-ground parts of medium-sized mature clonal Norway spruce trees were pretreated from the end of June till August with SSHB or BA or both. Needle retention of cuttings taken from the pretreated trees the following spring was prolonged until September and the cuttings flushed, but did not root, whereas cuttings from untreated stock plants died before September. No interaction between BA and SSHB was observed. When these pretreatments were repeated the following year on the same mature clonal trees rooting of cuttings was significantly improved in the 5 ppm SSHB treatment. 相似文献
7.
Tarit Kumar Baul Mohammad Mosharraf Hossain Mohammad Mezbahuddin Mohammed Mohiuddin 《林业研究》2011,22(3):409-416
In this study we investigated the rooting ability and the growth performance of juvenile single-node leafy stem cuttings of Litsea monopetala (Roxb) Pers. collected from two mature mother trees preserved in the hill forest of Chittagong district, Bangladesh. The rooting ability of cuttings was studied under 0%, 0.1%, 0.2% and 0.4% indole-3-butyric acid (IBA) treatments. Significantly better rooting response (p ≤ 0.05) was observed with 0.1% IBA compared to control (0% IBA). The mean number of roots and the length of the longest root of cuttings in different treatments showed no significant difference (p≤0.05). After transfer into polythene bags from non-mist propagator, rooted cuttings treated with 0%, 0.1% and 0.2% IBA demonstrated the highest (100 ± 0.00%) survival capacity. The mean number of shoots developed in cuttings in the polythene bags in first three weeks varied significantly (p≤0.05) among the treatments. Effects of three fertilizer treatments, viz. T0 (no fertilizer), T1 (10g Urea, 20g TSP, 10g MOP dissolved in 1 L water) and T2 (10g Urea, 20g TSP, 10g MOP dissolved in 2 L water) on initial growth ofstecklings were also measured over a 90-days period. The increment of leaf area of stecklings was significantly higher (p≤0.05) under T0 compared with that under T1 and T2 while the increment of stem length, collar diameter and root biomass varied insignificantly among different fertilizer treatments. The results suggest that rooting juvenile single-node leafy stem cuttings could be an effective mean of regenerating L. monopetala. The application of 0.1% IBA concentration is recommended for rooting of juvenile leafy stem cuttings and application of fertilizer appeared unnecessary for the subsequent growth of stecklings in polythene bags. 相似文献
8.
Cuttings from mature chestnut (Castanea spp.) trees are very difficult to root, whereas juvenile or etiolated cuttings root easily. The HPLC chromatograms from mature cuttings consistently showed two well-defined peaks that were absent from juvenile or etiolated cuttings. The compounds responsible for these two peaks were isolated and identified as derivatives of ellagic acid, 3,3',4-tri-O-methylellagic acid (compound 1) and 3,4,4'-tri-O-methylellagic acid (compound II). Compound I significantly inhibited rooting as measured by the bean test; a concentration of 3.0 x 10(-5) M caused a 32% inhibition in number of roots formed compared with controls grown in 10(-5) M IAA. When compound I was mixed with an equal quantity of IAA, the rooting activity of the auxin was reduced by 31.5%. An insufficient amount of compound II was available for the bean assay. 相似文献
9.
Rooting ability of cuttings from avocado (Persea americana Mill.) seedlings declined during the first 12 months of development. Cuttings from one-year-old seedlings rooted at a similar rate to cuttings prepared from mature plants. The amount of gibberellin-like substances in the leaves decreased during this time whereas abscisic acid content increased. Application of GA to avocado seedlings induced shoot elongation but failed to maintain the initial, high rooting ability. Treatments with ABA and AM0 1618 had no effect on either rooting or elongation. During the development of roots, the endogenous Avocado Rooting Promoter (ARP) accumulated at a faster rate in juvenile cuttings than in mature cuttings. 相似文献
10.
赤霉素及多效唑对3种草本花卉花期与株高的影响 总被引：3，自引：0，他引：3
采用不同质量浓度的赤霉素与多效唑对3种草本花卉金盏花、雏菊及三色堇的全株进行了喷施处理,分析了其对3种花卉开花起始时间、花期持续时间及株高的影响,主要研究结论是:(1)合适质量浓度的赤霉素喷施具有促使金盏花、雏菊及三色堇提前开花、缩短花期与增加株高的作用,而合适质量浓度的多效唑喷施正好相反,其具有推迟开花、延长花期与减小株高的作用;(2)在一定的范围内赤霉素的质量浓度与3种花卉花期的长短呈显著或极显著的负相关(p<0.05或p<0.01),而多效唑的质量浓度与3种花卉的花期呈正相关,其中三色堇的花期天数与多效唑质量浓度间的相关程度达极显著水平(p<0.01);(3)100 m g/L的赤霉素喷施可使金盏花、雏菊及三色堇分别提前18.0 d、11.0 d和12.4 d开花,并使其花期持续时间分别缩短3.7%、11.0%和18.3%,结实期株高分别增加86.6%、37.0%和101.6%;(4)100 m g/L的多效唑喷施可使金盏花、雏菊及三色堇分别推迟19.7 d、12.0 d和18.4 d开花,并使其花期持续时间分别延长11.0%、38.4%和31.1%,结实期株高分别减小39.4%、53.3%和41.6%. 相似文献
11.
奇异核桃组织培养嫩枝试管外生根苗移植3个月后进行地上及地下部分的生长情况调查.结果表明,根系修剪对植株地上部分的生长及根系的再生量没有显著影响,但是修剪处理明显减少了曲根的产生,尤其是重度修剪的处理根本没有曲根现象发生,半量修剪的处理有少量曲根发生,而不剪则出现很多曲根植株. 相似文献
12.
Techniques for the vegetative propagation of Ulmus villosa, an indigenous agroforestry tree species of the north-western sub-Himalayas, were developed. Studies investigated the effects on rooting of stem cuttings of growth regulators with sucrose and fungicide (0%, 0.2% p-HBA + 5% sucrose + 5% captan, and IBA concentrations ranging from 0.2 to 1.0% plus each containing, 0.2% p-HBA + 5% sucrose + 5% captan). Cuttings were collected at two times of the year (February and July) from two types of stock plant (seedling and mature trees) and from two positions within the shoots (lower and upper nodes). Cuttings collected in February were leafless, while those collected in July had about 80 cm2 leaf areas. These cuttings were then set under a mist unit. Percentage rooting and primary root number differed significantly between treated and untreated cuttings. Treatment of cuttings with chemical formulations of 0.4% IBA + 0.2% p-HBA + 5% sucrose + 5% captan maximized rooting (82.0%), while, survival (80%) and primary root number (14.7) were greatest with: (i) 0.6% IBA + 0.2% p-HBA + 5% sucrose + 5% captain and (ii) 0.8% IBA + 0.2% p-HBA + 5% sucrose + 5% captan, respectively. Rooting success and root number were better in propagules set in February rather than in July. Cuttings from seedlings rooted better, with more roots ensuring better survival, than cuttings from mature trees (12–13-years-old). In cuttings from seedlings, those cuttings collected from the lower part of the shoots had greater percentage rooting and primary root number than those from the upper part. Maximum root regeneration (100%), roots number (19.0), and survival (98.5%) were achieved when the time of collection (February) and shoot position were optimized. Rooting was best when cuttings had higher levels of sugar, total carbohydrate and peroxidase enzyme activity, and low N. 相似文献
13.
High-quality black walnut (Juglans nigra L.) logs are of great economic value and are used in the manufacture of high-end products. Indigenous to the central hardwood region, black walnut has been commercially cultivated for many years, and genetic improvement and selections have resulted in superior timber genotypes. The recalcitrance of black walnut cuttings to form adventitious roots is the greatest hurdle for mass propagation of improved material. The goal of this research was to improve the frequency of adventitious root formation in black walnut cuttings, and investigate anatomical changes during root development. Softwood cuttings (15–20 cm) were collected from juvenile and mature sources of elite genotypes, dipped for 60 s in 31.1, 62.2, or 93.2 mM indole-3-butyric acid-potassium salt (K-IBA), or 36.9, 73.8, or 110.7 mM indole-3-butyric acid (IBA), and then inserted into a moist medium consisting of 3 perlite: 1 coarse vermiculite (v/v). Cuttings were placed in bench-top fog chambers or a mist bench for 5 weeks. To visualize anatomical changes during root formation, stems were fixed in formaldehyde, embedded in paraffin, serially sectioned, and stained on sequential days throughout root development. Rooting was greatest (72%) for cuttings exposed to 93.2 mM K-IBA and placed in the fog chamber, while cuttings treated with IBA rooted at lower frequencies (16–22.2%). Cuttings in the mist bench often deteriorated and rooted at lower frequencies independent of the auxin type. Anatomical analysis revealed adventitious root initials by day 16 and root primordia formation by day 18. Rooted cuttings survived acclimatization to the greenhouse. 相似文献
14.
A. Itoh T. Yamakura S. Tan J. J. Kendawang H. S. Lee 《Journal of Forest Research》2003,8(2):0117-0121
Cuttings from older trees of the Dipterocarpaceae generally lose their ability to root. However, branches in a canopy of
adult dipterocarps are a possible source of cuttings because they show juvenile characteristics in architecture due to “adaptive
reiteration”, suggesting physiological rejuvenation. Effects of resource plant size on the rooting of cuttings and the possibility
of using cuttings from reiterated branches of adult trees were studied for Dryobalanops lanceolata, an emergent dipterocarp species. A cutting experiment with non-mist propagators was conducted for cuttings collected from
resource plants of four different size classes: <2 m, 2–5 m, 8–15 m, and 70 m in height. The smallest size class included
two different age classes: <2 and >2 years old. Cuttings from the tallest resource plant were collected from reiterated branches.
Rooting percentage was negatively correlated with resource plant size: 77–78% for resource plants <2 m, 63% for 2–5 m, 36%
for 8–15 m, and 0% for 70 m. Rooting percentages of cuttings collected from different individuals were not different for the
2–5 m tall class, while they were significantly different for the 8–15 m tall class. Resource plant size was negatively correlated
with the number of roots for rooted cuttings. No significant relationship was observed between resource plant size and mean
length of each root, total root length or total root dry weight for rooted cuttings. The results suggest the possibility of
collecting cuttings from relatively large resource plants up to 15 m tall and >20 years old if we chose good individuals for
resource plants. The results, however, show the difficulty in using reiterated branches of adult trees as a source of cuttings
for D. lanceolata.
Received: October 15, 2001 / Accepted: November 11, 2002
Acknowledgments We express our sincere thanks to Dr. S. Tamura, Dr. K. Ogino, and Mr. A.A. Hamid for their kind support. The tree tower was
constructed in a cooperative project between Japan and Sarawak supported by the Ministry of Education, Culture, Sports, Science
and Technology, Japan (Grant NP0201). The cutting experiment was partly funded by the Nippon Life Insurance Foundation and
the Japan Society for the Promotion of Science (JSPS-RFTF96R16001).
Correspondence to:A. Itoh 相似文献
15.
A study to determine the best vegetative propagation protocol was carried out for Dalbergia melanoxylon. Natural regeneration of D. melanoxylon is limited due to poor seed viability and germination. The effects of age of donor plant, indole-3-butyric acid (IBA) treatment
and cutting position on rooting of stem cuttings were investigated. The effect of age of donor plant was significant (P < 0.001) only for percent rooting, position effect was significant for all studied parameters, and IBA treatment at 300 ppm
was insignificant only for percent callusing. Interactive effects of cutting position × age of donor and cutting position × IBA
treatment were significant for percent rooting and root number per cuttings while interactive effect of age × IBA was significant
only for percent rooting. Observed difference in rooting performance could be due to uneven distribution of root promoting
and inhibiting substances with respect to age of donor plant and IBA which intensified root formation. Cuttings from juvenile
donor plant performed better in all rooting parameters than mature donor plant. The highest percentages in rooting were (71.11%)
and (24.42%) for cuttings from juvenile and mature donor plants, respectively. IBA treated cuttings produced higher percentage
rooting, number of roots and root length than untreated cuttings which revealed a strong IBA influence on rooting ability
of stem cuttings in D. melanoxylon. It was concluded that stem cuttings should be taken from juvenile donor plants and basal cutting position should be used
to produce high quality planting stock material for the afforestation and conservation programme of D. melanoxylon. 相似文献
16.
Maximization of short-rotation forest plantation yield requires frequent applications of nutrients, especially nitrogen (N). Whole-plant growth is known to be sensitive to the proportion of ammonium to nitrate (NH4:NO3). However, the extent to which N form affects root growth, branching and morphology is poorly understood, and these variables may have substantial impacts on plant nutrient and water acquisition. We used rooted cuttings of cottonwood (Populus deltoides Bartr. ex Marsh.) to investigate the effect of various NH4:NO3 ratios on root growth in N-enriched patches. A sand culture study with split-root systems was carried out in which 1-3% of the total root system of each cutting was supplied with 2 mM N at NH4:NO3 ratios of 0:100, 20:80, 40:60, 60:40, 80:20 and 100:0 (molar basis), with the rest of the plant supplied with 0 mM N, resulting in the whole plant becoming N deficient. During the experiment, whole-plant growth was unaffected by the treatments. Of the NH4:NO3 ratios tested, greatest total root length, specific root length, and root N concentration of roots in enriched patches occurred in the 20:80 NH4:NO3 treatment. The largest response of roots in enriched patches was third- and fourth-order root production. We conclude that N form has a profound effect on root development and morphology in enriched patches. 相似文献
17.
《林业研究》2021,(1)
The rooting capacity of Pinus massoniana is poor,especially for mature trees,and has prevented the development of clonal forestry for P.massoniana.In this study,we varied explant types,subculture times and exogenous hormones for plantlet regeneration and assessed shoots for rooting rate and root number for P.massoniana.Following five repetitive grafts,new shoots from grafts used as explant sources were rejuvenated as observed from juvenile shoot morphology and anatomy,leading to greatly enhanced plant regeneration in comparison to that of mature materials from 26-year-old P.massoniana trees.The rooting capacity of subcultured shoots increased with successive subcultures,reaching a peak at 20 subcultures with 35-40 days per subculture.However,rooting performance was significantly reduced after 30 subcultures.The addition of naphthaleneacetic acid(NAA) plus indoleacetic acid in the medium improved the root number,but the combination of exogenous NAA with paclobutrazol(PBZ) increased rooting rate and root number.We thus greatly improved the rooting capacity of mature P.massoniana trees by optimizing explant types(rejuvenated),subculture times(20 subcultures,35-40 days per subculture) and addition of NAA+PBZ to the rooting medium.The conditions can be used for efficient plantlet regeneration of P.massoniana. 相似文献
18.
Effects of genotype, collection date, auxin treatment, crown position and artificial chilling on rooting and subsequent vegetative growth of Cupressus sempervirens L. softwood cuttings taken from 13-year-old plants were evaluated. Three different clones (318, 296 and 22) previously selected for resistance to cypress cortical cancer caused by Seiridium cardinale were used. In all these clones, both the IBA treated and the untreated cuttings showed seasonal periodicity of rooting. Untreated cuttings rooted best when collected in April (up to 49% for the best clone, i.e. 296) and rooted very poorly in July and October. Auxin treatments appeared unable to overcome this periodicity, as the cuttings taken in July and October did not respond to IBA stimulation. However, in January and April, IBA treatments enhanced adventitious root formation of poorly rooting clones (318 and 22), as well as of clone 296, which performed best (89%) when cuttings were treated in April with 1.5% IBA. Cuttings of each clone collected in the lower third of the crown of the donor trees showed a significantly higher rooting percentage, when compared with those from the upper third. A 4-week post-severance chilling treatment (4°C) significantly enhanced the rooting capacity of cuttings taken in January, while a longer chilling treatment (8 weeks) had a negative effect. The subsequent growth of the rooted cuttings was not affected by either the crown position of the severed cuttings, or the genotype. 相似文献
19.
The present study aimed at examining the amenability of Stereospermum suaveolens DC. to vegetative propagation through rooting of leafy stem cuttings using low-cost polythene propagators. Rooting efficiencies
of cuttings derived from two selected matured mother trees were studied using four different concentrations of Indole-3-butyric
acid (IBA), viz. 0, 0.1, 0.2 and 0.4%. IBA applied at 40 μg per cutting (0.4% concentration) resulted in significantly higher
rooting responses compared to the control (P < 0.05). Differences in the length of longest root for all the treatments were significant (P = 0.05) with cuttings treated with 0.2% IBA producing the longest root. The highest survival after 3 weeks of transference
of the rooted cuttings into polythene bags was found for rooted cuttings treated with 0.2% IBA. Effects of three fertilizer
treatments viz. T0 (no fertilizer), T1 (10 g of Urea + 20 g of TSP + 10 g of MOP dissolved in 1 l of water) and T2 (10 g of Urea + 20 g of TSP + 10 g of MOP dissolved in 2 l of water) on initial growth of established rooted cuttings (stecklings)
of Stereospermum suaveolens in polythene bags were also measured for a period of 90 days. Stem length, collar diameter and leaf area of the stecklings
increased throughout the observation period. Increments of both stem length and collar diameter were significantly higher
for the stecklings treated with T2 in comparison with the control while increment of leaf area was found the highest for the stecklings treated with T1. The study clearly indicated that Stereospermum suaveolens is amenable to vegetative propagation technique using juvenile single-node leafy cuttings and the initial growth performance
of the established rooted cuttings (stecklings) in polythene bags under different fertilizer treatments was satisfactory. 相似文献
20.
Rapid vegetative propagation of promising multipurpose trees is an important need in agroforestry development. Grewia optiva andRobinia pseudoacacia are two such species, about the propagation of which very little is known. The rooting ability of stem cuttings harvested
from juvenile (2 year-old) and mature hardwood(15 year-old) trees of Robinia pseudoacacia andGrewia optiva was significantly influenced by the period or season of harvesting cutting. Juvenile cuttings of both species rooted significantly
better (42.9% in R. pseudoacacia and 46.6% in G. optiva) than mature hardwood cuttings (34.7% in R.pseudoacacia and 41.4% in G. optiva). The effect was more pronounced in auxin treated cuttings. InR. pseudoacacia, the highest rooting in juvenile(83.3%) and mature (66.6%) cuttings prepared in spring season was observed with the NAA (500
mg/l) treatment. InG. optiva, IBA (250 mg/l) in the monsoon season was most effective and recorded a maximum of 80% and 70%rooting in juvenile and mature
cuttings, respectively. The auxin treatments also significantly enhanced the number of roots (23.8 inR. pseudoacacia and 17.6 in G.optiva) and their mean length (14.3 cm inR. pseudoacacia and 16.1 cm inG. optiva). Interactions between age, season and treatments were significant at P < 0.05 level for rooting percent in R. pseudoacacia and non-significant for G. optiva. The results of this study suggest that it is possible to develop clones of genetically superior trees of R. pseudoacacia and G.optiva for use in agroforestry or afforestation programmes.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献