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神经肽Y免疫反应神经元在鸽小脑中的定位--SABC 法研究 总被引：3，自引：0，他引：3
采用石蜡切片和免疫组化SABC法（链霉亲合素-生物素-过氧化物酶连结法），对10只肉鸽小脑内神经肽Y免疫反应神经元的分布状况进行了研究．并与北京鸭、鸟鸡、肉鸡及大鼠的相关结果进行了比较。在光镜下观察分析了阳性神经元的分布状况．并用图像分析软件进行了半定量分析。结果显示：（1）小脑内神经肽Y（neuropeptide Y，NPY）阳性神经元主要存在于小脑皮质的蒲肯野氏细胞层，且以小叶顶端的蒲肯野氏细胞阳性明显；（2）小脑白质中央核可见到少量阳性神经元．不同于鸟鸡、肉鸡及大鼠；（3）分子层、颗粒层未见阳性反应细胞。表明：鸽小脑NPY阳性神经元的分布在皮质中与鸡、大鼠大体相似；在白质中与鸡、大鼠有差异。 相似文献
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乌鸡小脑的解剖学及神经肽Y神经元分布的研究 总被引：3，自引：0，他引：3
为了探明乌鸡小脑的形态结构及其细胞构筑和神经肽Y免疫反应神经元在小脑内的分布情况 ,采用石蜡切片HE染色和免疫组化SABC染色技术 ,对 1 0只泰和乌鸡的小脑进行了研究 ,并与北京鸭、肉鸽、本地鸡及非洲鸵鸟等禽类的相关结构进行了比较。结果表明 :①泰和乌鸡小脑皮质分 3层 ,由外至内依次为分子层、蒲肯野氏细胞层和颗粒层 ;②小脑分 1 0叶 ,Ⅰ、Ⅱ和Ⅹ为单叶 ,其余都有2个～ 3个小叶 ;③小脑中央核的神经元数量稀少 ;④神经肽Y免疫阳性神经元主要存在小脑皮质的蒲肯野氏细胞层 ,分子层、颗粒层 ,在小脑中央核未见阳性反应细胞 ;⑤泰和乌鸡小脑的形态结构及其细胞构筑与其它家禽相似 ;⑥小脑内NPY阳性神经元在蒲肯野氏细胞层的分布与鸡、肉鸽、大鼠的大体相似 ,在小脑白质内与肉鸽的有一定差异 相似文献
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本试验采用免疫组织化学方法对10头苏钟猪中枢神经系统Orexin B的分布定位进行了研究。结果表明,Orexin B免疫阳性神经元和纤维在猪下丘脑、脑干和脊髓均有分布。在下丘脑,阳性神经元和纤维分布于视前区、视交叉上核、室旁核、室周核、背内侧核、穹隆周核、乳头体核、外侧区和后区,其中以穹隆周区、背内侧核、未定带和下丘脑后区分布较密集,以视前区、视交叉上核、下丘脑外侧区和乳头体核分布较稀少。在脑干,阳性神经元分布于蓝斑、前庭神经核、下橄榄核、孤束核和疑核。在脊髓,阳性神经元较少,主要分布于颈部脊髓背角第Ⅳ层。此外,在斜角带、隔区、终纹床核等部位观察到密集的阳性纤维分布。本试验为进一步研究Orexin B的生理功能奠定了基础。 相似文献
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兔脑一氧化氮合酶(NOS)阳性神经元的形态、结构和分布 总被引：1，自引：0，他引：1
应用MADPH—d酶组织化学技术，对兔脑一氧化氮合酶（NOS）阳性神经元的形态、结构和分布进行了研究。结果显示：①NOS阳性神经元呈蓝色，细胞核不着色，突起染色很清晰；神经元胞体大多呈多角形、梭形，还有一些呈圆形或卵圆形等。②NOS阳性神经元几乎分布于家兔的各个脑区，包括大脑皮质、小脑、丘脑下部、中脑和脑桥。小脑分布最集中，而延髓分布较少。以上结果表明，NOS阳性神经元及其催化产生的一氧化氮（NO）与中枢神经系统的诸多功能有关。 相似文献
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利用SABC免疫组织化学方法,研究神经型一氧化氮合酶(nNOS)阳性神经元在家兔脑干中的分布和衰老变化。结果表明,家兔脑干内分布有丰富的nNOS阳性神经元;阳性神经元主要分布于动眼神经核、红核、脑桥核、三叉神经感觉主核以及脑干的网状结构等;在中央灰质周围、上丘(前丘)浅灰质层、臂旁核、中央上核、舌下神经核、下橄榄核、楔束核等核团也发现一些阳性神经元。对动眼神经核、红核、脑桥核、三叉神经感觉主核和延髓的外侧网状核这5个核团内阳性神经元的数量、胞体平均截面积和最长突起长度在5个年龄组的变化进行了比较。与成年兔相比,仔兔、青年兔阳性神经元的数量、胞体平均截面积和最长突起长度均没有显著性变化(P0.05),但老年兔(36月龄)阳性神经元的数量和最长突起长度都显著减少(P0.05),胞体平均截面积在动眼神经核、脑桥核、三叉神经感觉主核和外侧网状核减小,而在红核则增大(P0.05)。结果提示,脑干内丰富的nNOS阳性神经元,可能通过其生成的NO参与内脏活动、感觉和运动的传导以及睡眠和觉醒等脑的高级整合功能的调节;随着年龄的增长,nNOS阳性神经元的衰老变化会影响NO的合成与释放,从而影响它们在脑干中的正常生理功能。 相似文献
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对从胚胎期第6周到出生前山羊的延髓主要灰质核团发育的组织学变化进行了系统研究。结果表明：（1）第6周山羊胚胎延髓处在组织发生末期和核团形成初期，是其内部核团构建的关键时期。（2）延髓内不同核团中的神经元发育变化在时间上有较大的差异，在同一核团内神经元胞体发育分化在时间上也有差异，即同一核团内较为成熟的神经元胞体的数量由少到多，细胞质内的尼氏小体也存在由少到多，由小变大的过程。（3）延髓灰质结构形成和神经元发育分化的规律：有些结构发生早，而神经元分化较晚，如下橄榄核、三叉神经脊束核和孤束核；有些结构发生略晚，但其中的神经元胞体分化和发育则较早，如舌下神经核、疑核及延髓的网状结构；有些结构发生早，神经元发育也早，如迷走神经背核。 相似文献
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应用免疫组织化学SP法和原位杂交法研究了催产素（oxytocin，OT）及OT mRNA在成年发情期奶山羊下丘脑中的分布和表达。结果，OT免疫反应阳性细胞主要分布在视上核和室旁核，在视上弥散核、弓状核、室周核和乳头体各核团也存在免疫阳性神经元；在室旁核、视上弥散核、正中隆起和第三脑室附近有较多数量的强阳性神经纤维，在交叉上核有少量阳性神经纤维。在下丘脑23个核团（区）中均能检测出OT mRNA的阳性细胞。结果表明，OT和OT mRNA在下丘脑中分布广泛，且OT可能通过轴突传递和血液运输，将OT mRNA合成的OT运送到别的核团；OT在奶山羊发情过程中发挥了重要作用。 相似文献
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利用SABC免疫组织化学方法，对兔脑神经型一氧化氮合酶（nNOS）阳性神经元的形态、结构及分布规律进行了系统研究，结果表明：①nNOS阳性神经元呈深棕色，着色主要在胞质内，细胞核处较为浅淡；神经元胞体形态多种多样，有三角形、圆形、椭圆形、梭形等，神经元突起有一个或者数个；nNOS阳性神经纤维大多呈棕色串珠样，有些区域的阳性纤维交错分布，相互交织成网状。②家兔的各个脑区均有nNOS阳性神经元和神经纤维出现，其中在大脑皮质、小脑、丘脑下部、中脑和脑桥广泛分布，延髓分布极少。③nNOS阳性神经元，在仔兔时就已基本发育到成年兔水平，以后随着年龄增长逐渐发育成熟至衰老，到2岁龄（老年兔）时就出现了显著的衰老变化，即密度减小，表达强度减弱，第一级突起数减少，最长突起长度变短等。结果提示：nNOS阳性神经元及其催化产生的NO在家兔中枢神经系统的发育和神经调控中起重要作用。 相似文献
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Ovine scrapie was first recorded in Cyprus in 1985. Subsequently four dairy goats kept in two mixed flocks with affected sheep developed characteristic clinical signs similar to those seen in sheep. Fifteen goats from the two flocks were examined histologically and neurological lesions consistent with a diagnosis of scrapie were found in the four animals and in three others which had subsequently developed early neurological signs. These lesions were similar to those of naturally-affected sheep although neuronal degeneration and vacuolation were more severe in some cases. 相似文献
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Slocombe JO McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1985,26(10):323-327
In late December 1984, 1853 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1984 and 35% of them responded. Veterinarians reported that 97,794 dogs were blood-tested to check for microfilariae and 1417 dogs (1.45% of those tested) were found with heartworm. Another 34 dogs were amicrofilaremic, but were diagnosed as having heartworm disease, to give the total number diagnosed in 1984 as 1451 (1.48%). Heartworm was reported from all provinces except Prince Edward Island and Newfoundland but most (1310) of the cases were in Ontario. In Quebec, 126 cases were reported mostly from west of Montreal.
Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 27% of the cases were observed with clinical signs of heartworm disease and 72% had a history of not having left Canada. Southwestern Ontario continued to be the primary focus of the infection.
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Slocombe JO McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1984,25(9):347-350
In late December 1983, 2 800 veterinarians across Canada were sent a questionnaire in order to assess the status of heartworm disease in Canada in 1983 and 26% of them responded. Veterinarians reported that 59 504 dogs were blood-tested to check for microfilariae and 771 dogs (1.30% of those tested) were found with Dirofilaria immitis. Heartworm disease was diagnosed in all provinces except New Brunswick and Newfoundland but most (733) of the cases were in Ontario.
Heartworm disease was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 31% of the cases were observed with clinical signs of heartworm disease and 64% had a history of not having left Canada. Southwestern Ontario continues to be the focus of the infection and most of the dogs there had not left the province previously.
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Slocombe JO Villeneuve A 《The Canadian veterinary journal. La revue veterinaire canadienne》1993,34(10):630-633
In late November 1991, 1883 clinics in Canada were sent a questionnaire to assess the status of Dirofilaria immitis in dogs in 1991 and there was a 60.0% response. There were 344,031 dogs tested for heart-worm (HW), 627 were found infected and the prevalence of HW infection was 0.18%. There were 417 dogs with HW in Ontario, 116 in Manitoba, 38 in Quebec, 53 in British Columbia, three in Alberta, and one in Nova Scotia. In British Columbia, all of the infected dogs but one were from the Okanagan valley which, as from 1991, is a new focus of infection in Canada. Most dogs with HW had not been on preventive medication in 1990, and the prevalence among dogs tested and unprotected was 0.59%. That prevalence was considerably higher in endemic areas. Companion dogs, over three years of age and maintained primarily outdoors in rural areas, were most frequently infected. One cat was diagnosed with D. immitis and 33 dogs had Dipetalonema reconditium. 相似文献
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Owen J Slocombe D McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1986,27(9):324-328
In late December 1985, 1485 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1985 and 44% of them responded. Veterinarians reported that 137,300 dogs were blood-tested to check for microfilariae and 1210 dogs were found with heartworm. Another 36 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number diagnosed in 1985 as 1247 (0.91%).
Heartworm was reported from all provinces except Prince Edward Island, Newfoundland and Saskatchewan but most (1126) of the cases were in Ontario. Southwestern Ontario continued to be the primary focus of the infection in Canada. From Quebec, 91 cases were reported mostly from and around Montreal. From Manitoba, 19 cases were reported from Winnipeg and surrounding areas. Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 28% of the cases were observed with clinical signs of heartworm disease and 78% had a history of not having left Canada.
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Slocombe JO McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1989,30(6):504-508
In late November 1988, 1581 small and mixed animal clinics and institutional veterinarians across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1988, and 46% of them responded. Veterinarians reported that 181,577 dogs were blood-tested for heartworm disease and 367 dogs were found with D. immitis microfilariae. Another 60 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number of cases diagnosed in 1988 as 441 (0.24%). 相似文献
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In late November 1989, 1732 clinics and institutional veterinarians were sent a questionnaire to assess the status of Dirofilaria immitis, and 51.7% responded. Of 247,716 dogs tested, 394 had D. immitis microfilariae and 51 were amicrofilaremic for a total of 445 cases and heartworm prevalence of 0.17%. Most (408) of these dogs had no preventive medication and the prevalence among dogs tested and unprotected was 1.01%. That prevalence was considerably higher in endemic areas. Thirty-seven dogs with heartworm had preventive medication. Heartworm was most frequent in companion dogs over three years of age maintained outdoors in rural areas. About 75% of the cases had never left Canada, 26% had clinical signs and 125 were not treated.
Heartworm was reported from British Columbia, Manitoba, Ontario, Quebec, Nova Scotia and Newfoundland, but 383 cases were in Ontario. South-western Ontario was the primary focus of infection. There were 33 cases in Quebec and 24 in Manitoba, mainly found in and around Metropolitan Montreal and Winnipeg respectively.
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