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1.
Weed populations with resistance to glyphosate have evolved over the last 7 years, since the discovery of the first glyphosate‐resistant populations of Lolium rigidum in Australia. Four populations of L. rigidum from cropping, horticultural and viticultural areas in New South Wales and South Australia were tested for resistance to glyphosate by dose–response experiments. All populations required considerably more glyphosate to achieve 50% control compared with a known susceptible population, indicating they were resistant to glyphosate. Translocation of glyphosate within these resistant populations was examined by following the movement of radiolabelled glyphosate applied to a mature leaf. All resistant plants translocated significantly more herbicide to the tip of the treated leaf than did susceptible plants. Susceptible plants translocated twice as much herbicide to the stem meristematic portion of the plant compared with resistant plants. These different translocation patterns suggest an association between glyphosate resistance in L. rigidum and the ability of glyphosate to accumulate in the shoot meristem.  相似文献   

2.
BACKGROUND: Resistance to glyphosate in weed species is a major challenge for the sustainability of glyphosate use in crop and non‐crop systems. A glyphosate‐resistant Italian ryegrass population has been identified in Arkansas. This research was conducted to elucidate its resistance mechanism. RESULTS: The investigation was conducted on resistant and susceptible plants from a population in Desha County, Arkansas (Des03). The amounts of glyphosate that caused 50% overall visual injury were 7 to 13 times greater than those for susceptible plants from the same population. The EPSPS gene did not contain any point mutation that has previously been associated with resistance to glyphosate, nor were there any other mutations on the EPSPS gene unique to the Des03 resistant plants. The resistant plants had 6‐fold higher basal EPSPS enzyme activities than the susceptible plants, but their I50 values in response to glyphosate were similar. The resistant plants contained up to 25 more copies of EPSPS gene than the susceptible plants. The level of resistance to glyphosate correlated with increases in EPSPS enzyme activity and EPSPS copy number. CONCLUSION: Increased EPSPS gene amplification and EPSPS enzyme activity confer resistance to glyphosate in the Des03 population. This is the first report of EPSPS gene amplification in glyphosate‐resistant Italian ryegrass. Other resistance mechanism(s) may also be involved. Copyright © 2012 Society of Chemical Industry  相似文献   

3.
BACKGROUND: Glyphosate‐resistant (GR) weed species are now found with increasing frequency and threaten the critically important GR weed management system. RESULTS: The reported 31P NMR experiments on glyphosate‐sensitive (S) and glyphosate‐resistant (R) horseweed, Conyza canadensis (L.) Cronq., show significantly more accumulation of glyphosate within the R biotype vacuole. CONCLUSIONS: Selective sequestration of glyphosate into the vacuole confers the observed horseweed resistance to glyphosate. This observation represents the first clear evidence for the glyphosate resistance mechanism in C. canadensis. Copyright © 2010 Society of Chemical Industry  相似文献   

4.
Glyphosate has been associated with control failures for Spermacoce verticillata in some Brazilian States. The objective of this work was to evaluate and determine the possible causes of this and propose alternative herbicides to use. Glyphosate was applied at three plant stages of development (2–4 leaves, 4–6 leaves and full bloom) where foliar absorption and translocation of glyphosate to various plants parts and time were analysed using the 14C technique. Data were submitted to nonlinear regressions and analysis of variance, where means were compared by a Tukey test at 5% probability. Plant control by the application of different herbicides (19) in the same three stages of development of weed was evaluated. Twenty‐one days after herbicide application, control was visually evaluated and data analysed and means were compared. Due to absorption and/or translocation problems, S. verticillata plants were not controlled by glyphosate. Plants with 4–6 leaves showed lower absorption and translocation of the herbicide to the leaf/root regions compared with plants at the beginning of their development. Plants at full bloom showed lower translocation of the herbicide to the root. In addition to the application of glyphosate at early stages of development, the application of paraquat, flumioxazin and mixtures of glyphosate with flumioxazin or cloransulam is recommended. Late applications could result in poor control, principally if glyphosate is used. Therefore, early applications are strongly recommended for control of this species.  相似文献   

5.
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6.
Lolium rigidum (annual or rigid ryegrass) is a widespread annual weed in cropping systems of southern Australia, and herbicide resistance in L. rigidum is a common problem in this region. In 2010, a random survey was conducted across the grain belt of Western Australia to determine the frequency of herbicide‐resistant L. rigidum populations and to compare this with the results of previous surveys in 1998 and 2003. During the survey, 466 cropping fields were visited, with a total of 362 L. rigidum populations collected. Screening of these populations with the herbicides commonly used for control of L. rigidum revealed that resistance to the ACCase‐ and ALS‐inhibiting herbicides was common, with 96% of populations having plants resistant to the ACCase herbicide diclofop‐methyl and 98% having plants resistant to the ALS herbicide sulfometuron. Resistance to another ACCase herbicide, clethodim, is increasing, with 65% of populations now containing resistant plants. Resistance to other herbicide modes of action was significantly lower, with 27% of populations containing plants with resistance to the pre‐emergent herbicide trifluralin, and glyphosate, atrazine and paraquat providing good control of most of the populations screened in this survey. Ninety five per cent of L. rigidum populations contained plants with resistance to at least two herbicide modes of action. These results demonstrate that resistance levels have increased dramatically for the ACCase‐ and ALS‐inhibiting herbicides since the last survey in 2003 (>95% vs. 70–90%); therefore, the use of a wide range of integrated weed management options are required to sustain these cropping systems in the future.  相似文献   

7.
In Shizuoka Prefecture, Japan, glyphosate‐resistant Lolium multiflorum is a serious problem on the levees of rice paddies and in wheat fields. The mechanism of resistance of this biotype was analyzed. Based on LD50, the resistant population was 2.8–5.0 times more resistant to glyphosate than the susceptible population. The 5‐enolpyruvyl‐shikimate‐3‐phosphate synthase (EPSPS) gene sequence of the resistant biotype did not show a non‐synonymous substitution at Pro106, and amplification of the gene was not observed in the resistant biotype. The metabolism and translocation of glyphosate were examined 4 days after application through the direct detection of glyphosate and its metabolite aminomethylphosphonic acid (AMPA) using liquid chromatograph‐tandem mass spectrometer (LC‐MS/MS). AMPA was not detected in either biotype in glyphosate‐treated leaves or the other plant parts. The respective absorption rates of the susceptible and resistant biotypes were 37.90 ± 3.63% and 41.09 ± 3.36%, respectively, which were not significantly different. The resistant biotype retained more glyphosate in a glyphosate‐treated leaf (91.36 ± 1.56% of absorbed glyphosate) and less in the untreated parts of shoots (5.90 ± 1.17%) and roots (2.76 ± 0.44%) compared with the susceptible biotype, 79.58 ± 3.73%, 15.77 ± 3.06% and 4.65 ± 0.89%, respectively. The results indicate that the resistance mechanism is neither the acquisition of a metabolic system nor limiting the absorption of glyphosate but limited translocation of the herbicide in the resistant biotype of L. multiflorum in Shizuoka Prefecture.  相似文献   

8.
Echinochloa colona is the most common grass weed of summer fallows in the grain‐cropping systems of the subtropical region of Australia. Glyphosate is the most commonly used herbicide for summer grass control in fallows in this region. The world's first population of glyphosate‐resistant E. colona was confirmed in Australia in 2007 and, since then, >70 populations have been confirmed to be resistant in the subtropical region. The efficacy of alternative herbicides on glyphosate‐susceptible populations was evaluated in three field experiments and on both glyphosate‐susceptible and glyphosate‐resistant populations in two pot experiments. The treatments were knockdown and pre‐emergence herbicides that were applied as a single application (alone or in a mixture) or as part of a sequential application to weeds at different growth stages. Glyphosate at 720 g ai ha?1 provided good control of small glyphosate‐susceptible plants (pre‐ to early tillering), but was not always effective on larger susceptible plants. Paraquat was effective and the most reliable when applied at 500 g ai ha?1 on small plants, irrespective of the glyphosate resistance status. The sequential application of glyphosate followed by paraquat provided 96–100% control across all experiments, irrespective of the growth stage, and the addition of metolachlor and metolachlor + atrazine to glyphosate or paraquat significantly reduced subsequent emergence. Herbicide treatments have been identified that provide excellent control of small E. colona plants, irrespective of their glyphosate resistance status. These tactics of knockdown herbicides, sequential applications and pre‐emergence herbicides should be incorporated into an integrated weed management strategy in order to greatly improve E. colona control, reduce seed production by the sprayed survivors and to minimize the risk of the further development of glyphosate resistance.  相似文献   

9.
The weed species, prickly sida (Sida spinosa L.) and sicklepod (Senna obtusifolia L.), were treated with 14C-glyphosate alone and formulated with different polyethlylane oxide (PEO) surfactants in tallow amine ethoxylate and non-ionic alkoxylate series to determine the amount of 14C-glyphosate absorption and translocation. The surface tension, contact angle, and 14C-glyphosate distribution were significantly affected by both the presence of different waxes on the plants and by the addition of surfactants to the glyphosate. The surface and contact angle values of the surfactants, with and without glyphosate, showed a significant increase as the PEO number increased in both surfactant series. A higher absorption of the 14C-glyphosate was recorded for S. spinosa compared with S. obtusifolia. The absorption and translocation of the 14C-glyphosate increased with the increase in the PEO number of tallow amine ethoxylate. In the case of the non-ionic alkoxylate surfactant series, an increase in the absorption of 14C-glyphosate was recorded when the surface tension and contact angle values decreased. There was no significant difference in the translocation values obtained in the two species after the addition of the surfactants. The amount of 14C-glyphosate absorbed by the treated leaf was significantly higher in the case of S. spinosa compared with S. obtusifolia. A linear relationship was observed with the physical properties, 14C absorption, and the efficacy of glyphosate with the addition of the non-ionic alkoxylate surfactant series. The percentage control was higher with the higher PEO surfactant in the tallow amine ethoxylate surfactant series and with the lower PEO surfactant in the non-ionic alkoxylate surfactant series as the two series are chemically different.  相似文献   

10.
Glyphosate‐resistant Palmer amaranth from Georgia (GA), USA, possesses multiple copies of the gene that encodes 5‐enolpyruvylshikimate‐3‐phosphate synthase (EPSPS), the enzyme target site of this herbicide. The cloned plants of glyphosate‐resistant and glyphosate‐susceptible Palmer amaranth biotypes from Mississippi (MS), USA, and GA were evaluated for glyphosate injury (digital imaging) in leaf disc bioassays. Four groups (three resistant groups: two from MS [G and R] and one from GA [C7]; one susceptible group from GA [C3]) were chosen for cloning to facilitate long‐term studies. After exposure to glyphosate (1.0 mmol L–1, 144 h), the level of injury (mean value) was low in the resistant groups, while a higher level of injury was found in the susceptible group. However, the individual injury values within all groups varied widely. The mean EPSPS gene copy number of these groups was G ≥ R > C7 >>> C3. However, a higher copy number did not always convey increased resistance in these bioassays. When the copy number was high (>20), 81.5% of the bioassayed plants exhibited little or no injury and only ～20% were significantly injured, while 50% of the plants with a low copy number (<20) remained healthy. Overall, no strong statistical correlation of the copy number versus injury occurred in these cloned plants and no statistical relationship of resistance and copy number with the sex of the MS plants was observed. The results suggest that although an elevated copy number of the EPSPS gene can instill resistance, other mechanisms might contribute to the overall glyphosate resistance of Palmer amaranth in these plants.  相似文献   

11.
Annual ryegrass (Lolium rigidum) is the only weed species to have evolved resistance to the broad‐spectrum herbicide glyphosate in Australia. A population that had failed to be controlled by glyphosate was collected from a vineyard in the Adelaide Hills region of South Australia. Dose–response experiments on this population (SLR 77) showed that it was glyphosate resistant, with an LD50 that was 1.9–3.4 times higher than that of a susceptible population (VLR 1). The movement of radiolabelled glyphosate within SLR 77 plants showed that this population did not have the differential glyphosate translocation mechanism of resistance common to several other Australian glyphosate‐resistant populations. Subsequent analysis of shikimic acid accumulation within the plant after glyphosate treatment showed that this population accumulated significantly less shikimic acid than a susceptible population, but more than a glyphosate‐resistant population with the translocation mechanism, indicating the possible involvement of another mechanism of resistance. Sequencing of a portion of the SLR 77 5‐enolpyruvylshikimate‐3‐phosphate synthase gene was carried out and a mutation causing an amino acid change at position 106 from proline to threonine was identified. This mutation is likely to be responsible for glyphosate resistance in this population, as mutations in this position have been found to be responsible for glyphosate resistance in goosegrass (Eleusine indica) from Malaysia. This paper represents the first report of target‐site glyphosate resistance in L. rigidum and provides evidence that this species has at least two mechanisms of glyphosate resistance present in Australia.  相似文献   

12.
Annual ryegrass (Lolium rigidum) is a widespread and important weed of Australia and populations of this weed have developed resistance to most major herbicides, including glyphosate. The possible mechanisms of resistance have been examined in one glyphosate-resistant Lolium population. No major differences were observed between resistant and susceptible biotypes in respect of (i) the target enzyme (EPSP synthase), (ii) DAHP synthase, the first enzyme of the target (shikimate) pathway, (iii) absorption of glyphosate, or (iv) translocation. Following treatment with glyphosate, there was greater accumulation of shikimate (derived from shikimate-3-Pi) in susceptible than in resistant plants. In addition, the resistant population exhibited cross-resistance to 2-hydroxy-3-(1,2,4-triazol-1-yl)propyl phosphonate, a herbicide which, although structurally similar to glyphosate, acts at an unrelated target site. On the basis of these observations we speculate that movement of glyphosate to its site of action in the plastid is involved in the resistance mechanism. © 1999 Society of Chemical Industry  相似文献   

13.
Saflufenacil is a new herbicide on the market and its effectiveness on horseweed, several populations of which have evolved resistance to glyphosate, is not clear. In this research, the effect of adjuvants on the control of horseweed with saflufenacil in the field, the effect of the interaction between glyphosate and saflufenacil on glyphosate‐resistant and glyphosate‐susceptible horseweed and the patterns of uptake and translocation of glyphosate applied alone and in combination with saflufenacil in horseweed were evaluated. The addition of methylated seed oil to saflufenacil provided the best control of horseweed, with crop oil concentrate being intermediate in effect and non‐ionic surfactant ranking as the least‐effective adjuvant. The interaction between glyphosate and saflufenacil was additive with regards to the control of glyposate‐resistant horseweed. The glyphosate‐susceptible horseweed population absorbed 6–13% more 14C‐glyphosate than the glyphosate‐resistant population. The addition of saflufenacil reduced 14C‐glyphosate translocation in both the glyphosate‐resistant and the glyphosate‐susceptible horseweed populations by at least 6%; however, due to the exceptional efficacy of saflufenacil, these reductions did not reduce the level of control. Saflufenacil holds great potential as an alternative control option for glyphosate‐resistant horseweed and is a valuable tool in the management of resistant weeds.  相似文献   

14.
Experiments evaluated the effect of glyphosate rate and Anoda cristata density, on crop and weed biomass and weed seed production in wide (70 cm) and narrow rows (35 cm) glyphosate‐resistant soyabean (Glycine max). Soyabean density was higher at 35 cm row spacing as an increase in planting rate in narrow‐row soyabean is recommended for producers in Argentina. Soyabean biomass at growth stage V4 (four nodes on the main stem with fully developed leaves beginning with the unifoliate leaves) was higher when grown on narrow than in wide‐rows but was not affected by the presence of A. cristata. At growth stage R5 (seed initiation – seed 3 mm long in a pod at one of the four uppermost nodes on the main stem, with a fully developed leaf and full canopy development), crop biomass was greater in narrow rows compared with wide rows with 12 plants m?2 of A. cristata. In narrow‐row soyabean, a single application of a reduced rate of glyphosate maintained soyabean biomass at R5 and provided excellent weed control regardless of weed density. In wide‐row soyabean control was reduced at the high weed density. Regardless of row spacing, A. cristata biomass and seed production were severely reduced by half of the recommended dose rate of glyphosate but the relationship between biomass and seed production was not altered. Glyphosate rates as low as 67.5 g a.e. ha?1 in narrow rows or 540 g a.e. ha?1 in wide rows provided excellent control of A. cristata. To minimize glyphosate use, planting narrow‐row soyabean are effective where A. cristata density is low.  相似文献   

15.
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16.
Separate field experiments were conducted to evaluate Sorghum halepense (L.) Pers. (johnson-grass) control with foliar-applied herbicides in soyabeans (Glycine max (L.) Merr. cv. Williams) grown in tilled (mould board plough) or no-tillage fields. Fluazifop-butyl, sethoxydim, CGA-82725 and mefluidide were applied post-emergence to 45-cm high S. halepense and glyphosate was applied with a wick bar to 90-cm high S. halepense in the conventional and no-tillage studies. HOE-00581 {ethyl 2-[4-[(6-chloro-2-benzoazolyl)oxyl]-phenoxy]propanoate} was applied to 45-cm high S. halepense only in the no-tillage studies. Fluazifop-butyl, sethoxydim, CGA-82725, HOE-00581 (no-tillage only) and glyphosate provided good control of S. halepense in conventional and no-tillage soyabeans and generally increased soyabean yields compared to weedy controls, when low precipitation was not limiting yields. Mefluidide at 0.3 kg ai ha?1 provided the least S. halepense control in the conventional and no-tillage studies. The studies were not designed to compare S. halepense control in the two tillage systems; however, these data show that tillage to disrupt S. halepense rhizomes was not required to obtain good control of S. halepense with foliar applications of fluazifop-butyl, sethoxydim, CGA-82725, HOE-00581 and glyphosate. La lutte contre Sorghum halepense (L.) Pers. utilisant des herbicides foliaires dans le soja en culture classique et en non-culture Plusieurs essais ont été installés sur le terrain dans le but d'évaluer des herbicides foliaires pour la destruction de Sorghum halepense (L.) Pers. dans le soja (Glycine max (L.) Merr. var. Williams) cultivé en terre labourée et en non-culture. Le fluazifop-butyle, le séthoxydime, le CGA-82725 et le méfluidide ont été appliqués en postlevée sur S. halepense haut de 45 cm et le glyphosate a été appliqué sur S. halepense haut de 90 cm, utilisant un frotteur, dans les deux sytèmes de culture. HOE-00581 (éthyle 2-[4-[(6-chloro-2-benzoazolyl)oxyl]phénoxy]propanoate) a été appliqué sur S. halepense haut de 45 cm uniquement dans les systèmes de·non-culture. Le fluazifop-butyle, le séthoxydime, le CGA-82725, le HOE-00581 et le glyphosate ont bien maitriséS. halepense dans les deux systèmes de culture du soja, tout en augmentant les rendements de soja par rapport au témoin enherbé, là où une précipitation insuffisante n'est pas intervenue. Le méfluidide à 0,3 kg ai ha?1 a donné la plus faible destruction de S. halepense en culture classique et en non-culture. Une comparaison de la destruction de S. halepense dans les deux systèmes de culture n'était pas le but principal de ces travaux; cependant ces données font voir qu'un labourage pour briser les rhizomes de S. halepense n'est pas de rigueur pour obtenir une bonne maîtrise de l'adventice par des applications foliaires de fluazifop-butyle, séthoxydime, CGA-82725, HOE-00581 et glyphosate. Sorghum halepense (L.) Pers. Bekämpfung mit blattwirksamen Herbiziden in Soyakulturen mit und ohne Bodenbearbeitung Es wurden separate Feldversuche zur Bekämpfung von Sorghum halepense (L.) Pers. mit blattwirksamen Herbiziden in Soyakulturen (Glycinemax (L.) Merr. cv. Williams), ohne und mit Bodenbearbeitung (Mouldboard Pflug), ausgeführt. Fluazifop-Butyl, Sethoxydim, CGA-82725 und Mefluidid wurden postemergent auf 45 cm hohe und Glyphosat mit einem Docht-Streichgerät (wick bar) auf 90 cm hohe S. halepense appliziert; diese Produkte kamen in den Feldern mit und ohne Bodenbearbeitung zur Anwendung. HOE-00581 {Aethyl 2-[4-[(6-chlor-2-benzoazolyl)oxyl]phenoxy]propanoat} wurde nur auf den Parzellen ohne Bodenbearbeitung auf 45 cm hohe S. halepense appliziert. Fluazifop-Butyl, Sethoxydim, CGA-82725, HOE-00581 (Ohne Bodenbearbeitung) und Glyphosat erzielten in bearbeiteten und unbearbeiteten Feldern eine gute Bekämpfung von S. halepense und bewirkten im allgemeinen eine Erhöhung der Ernteerträge, sofern nicht geringe Niederschläge als limitierender Faktor auftraten. Mefluidid (0,3 kg ai ha?1) ergab die geringste Wirkung in bearbeiteten und unbearbeiteten Feldern. Diese Versuche waren nicht geplant um den Erfolg der S. halepense Bekämpfung unter den zwei Anbaubedingungen (mit resp. ohne Bodenbearbeitung) miteinander zu vergleichen; immerhin zeigen die Ergebnisse, dass eine Bodenbearbeitung zur Zerkleinerung der S. halepense-Rhizome nicht notwendig ist, um gute Effekte mit Fluazifop-Butyl, Sethoydim, CGA-82725, HOE-00581 und Glyphosat zuerzielen.  相似文献   

17.
Resistance to glyphosate and paraquat has evolved in some populations of Conyza spp. from California, USA. This study evaluated whether herbicide absorption and translocation were involved in the mechanism of resistance to both herbicides. Three lines of each species were used: glyphosate‐paraquat‐susceptible (GPS), glyphosate‐resistant (GR) and glyphosate‐paraquat‐resistant (GPR). Radiolabelled herbicide was applied to a fully expanded leaf, and absorption and movement out of the treated leaf were monitored for up to 24 h for paraquat and 72 h for glyphosate. Plants treated with paraquat were incubated in darkness for the first 16 h and then subjected to light conditions. More glyphosate was absorbed in C. bonariensis (52.9–58.3%) compared with C. canadensis (28.5–37.6%), but no differences in absorption were observed among lines within a species. However, in both species, the GR and GPR lines translocated less glyphosate out of the treated leaf when compared with their respective GPS lines. Paraquat absorption was similar among lines and across species (71.3–77.6%). Only a fraction of paraquat was translocated in the GPR lines (3% or less) when compared with their respective GPS or GR lines (20% or more) in both species. Taken together, these results indicate that reduced translocation is involved in the mechanism of resistance to glyphosate and paraquat in C. bonariensis and C. canadensis.  相似文献   

18.
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19.
Parietaria debilis is a dominant annual weed in many non‐tillage fallow and undisturbed areas in Argentina. Herbicides control P. debilis inconsistently. A study on the growth of P. debilis, in a fallow in a soyabean–maize rotation including glyphosate‐resistant cultivars and in an undisturbed area without crops or weed control, was conducted in the central soyabean area of Argentina. The biomass dynamics of all species in the community, as well as biomass, density and growth traits of P. debilis were assessed. Biomass of several species including P. debilis and total biomass were greater in undisturbed than in fallow plots in both years of the study. Diversity was greater in undisturbed plots in 2000, but no differences in diversity were observed in 2001. In both treatments, P. debilis emergence began in March and finished in October and density increased from March to May. High mortality occurred in June and July, particularly in undisturbed plots. In the fallow plots, no other tall weed species were present and light interception was higher in spring when fruit production was at its peak. Conversely, in the undisturbed plots, P. debilis was substantially taller and had greater leaf area due to lower light interception by the high canopy of the rest of the community. In fallow, P. debilis is favoured by single applications of glyphosate, because the herbicide eliminates competitors. Control, if applied, should be directed to both fallow and undisturbed environments, as the weed produces considerable numbers of seeds and can invade extensively managed areas.  相似文献   

20.
Field experiments were conducted in northern Greece in 2003 and 2004 to evaluate effects of tillage regimes (moldboard plowing, chisel plowing, and rotary tilling), cropping sequences (continuous cotton, cotton‐sugar beet rotation, and continuous tobacco) and herbicide treatments with inter‐row hand hoeing on weed population densities. Total weed densities were not affected by tillage treatment except that of barnyardgrass (Echinochloa crus‐galli), which increased only in moldboard plowing treated plots during 2003. Redroot pigweed (Amaranthus retroflexus) and black nightshade (Solanum nigrum) densities were reduced in continuous cotton, while purple nutsedge (Cyperus rotundus), E. crus‐galli, S. nigrum, and johnsongrass (Sorghum halepense) densities were reduced in tobacco. A. retroflexus and S. nigrum were effectively controlled by all herbicide treatments with inter‐row hand hoeing, whereas E. crus‐galli was effectively reduced by herbicides applied to cotton and tobacco. S. halepense density reduction was a result of herbicide applied to tobacco with inter‐row hand hoeing. Yield of all crops was higher under moldboard plowing and herbicide treatments. Pre‐sowing and pre‐emergence herbicide treatments in cotton and pre‐transplant in tobacco integrated with inter‐row cultivation resulted in efficient control of annual weed species and good crop yields. These observations are of practical relevance to crop selection by farmers in order to maintain weed populations at economically acceptable densities through the integration of various planting dates, sustainable herbicide use and inter‐row cultivation; tools of great importance in integrated weed management systems.  相似文献   

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