共查询到19条相似文献，搜索用时 250 毫秒
1.
布尔山羊超数排卵胚胎移植试验 总被引：2，自引：0，他引：2
本试验采用置入炔诺酮阴道栓 FSH 氯前列烯醇同期发情、超数排卵处理方法，对8只布尔山羊(供体羊)、90只洪洞奶山羊(受体羊)进行了同期发情和超排数排卵胚胎移植试验。其结果如下：(1)8只供体羊回收胚胎数量、可用胚胎数量、羊均回收胚胎数量、羊均可用胚胎数量、可用胚比率分别为182枚、172枚、22．8枚、21．5枚、94．5％；(2)90只受体羊共有81只羊达到同期发情效果，发情同期率为90．0％；(3)150枚可用胚胎共移植洪洞奶山羊73只，妊娠51只，产羔80只，妊娠率69．9％，胚胎成羔率53．5％。 相似文献
2.
[目的]研究和牛母牛超数排卵及胚胎移植效果。[方法]利用纯种和牛母牛作为供体,荷斯坦母牛及西杂母牛作为受体,通过供体牛的超数排卵、人工授精、采胚、胚胎冷冻及受体牛的同期发情和鲜胚移植,快速获得纯种和牛及和牛胚胎。[结果]对4头青年纯种和牛母牛进行超排,重复冲卵13头次,共采卵数122枚,其中75枚可用胚胎,可用胚率为61.5%,平均5.76枚/头次。对75头荷斯坦牛及西杂母牛同期发情,结果获得89.2%的同期发情率。67头受体牛移植了鲜胚,2个月后妊娠检查有35头怀孕,移植妊娠率为52.23%。[结论]可以利用超数排卵及胚胎移植增加和牛母牛利用效率。 相似文献
3.
为了充分利用优良母牛遗传资源,发挥超数排卵技术对改良奶牛品种的重要作用,采用CUE-MATE孕酮栓+FSH+PGF2α法和自然发情+FSH+PGF2α法对酒泉地区饲养的奶牛进行了超数排卵研究。结果表明:自然发情法和CUE-MATE法超排回收的平均可用胚胎数分别为4.54枚和4.86枚;上、中、下3等膘情供体牛获得的平均可用胚胎数分别为6.13、5.39和3.29枚,可用胚率分别为58.60%、58.08%和44.66%;采用CUE-MATE孕酮栓+FSH+PGF2α方法进行超排处理,平均可用胚胎数:春季(4.00枚)≤秋季(4.86枚)≤冬季(5.07枚),平均可用胚率:秋季(56.04%)>冬季(50.71%)>春季(43.50%);青年母牛的超排效果略高于经产母牛,但两者间差异不显著(P>0.05)。 相似文献
4.
《上海畜牧兽医通讯》2016,(2)
采用杂交奶水牛及纯种奶水牛作为供体牛,将日本生产的FSH作为外源激素进行超数排卵,以确定胚胎移植供体牛超排的效果;采用CIDR+PMSG+PG+LHRH-A3法,确定受体牛胚胎移植和同期发情的效果。试验结果表明:5头奶水牛供体牛超排,共采卵7头次,采卵数21枚,其中10枚可用胚胎,平均1.4枚/头次,可用胚率为47.6%。受体牛的同期发情效果和可移植率分别为77.0%和62.0%。 相似文献
5.
21世纪以来,贵州开展了牛同期发情、超数排卵、胚胎分割、早期胚胎性别鉴定、胚胎移植等试验及其示范应用。研究表明:结合使用孕酮阴道栓和氯前列烯醇进行同期发情,受体合格率分别为50.12%(423/840)和37.82%(247/653),同期发情率分别为81.09%(343/423)和65.58%(162/247),受体妊娠率分别为51.52%(136/268)和18.57%(26/140),胚胎移植总效率分别为16.36%和3.98%,前者均极显著高于后者(P<0.01);促卵泡素法处理供体平均获得可用胚胎数4·8枚(43/9),促卵泡素 雌二醇法处理供体获可用胚9.8枚(117/12),差异极显著(P<0.01),说明促卵泡素和雌二醇结合使用进行超排,可改善牛的超排效果,显著增加可用胚胎数;8、10月份移植妊娠率分别为40.34%(48/119)、45.41%(84/185),均高于12月份的24.80%(26/105),且差异显著(P<0.05),可见,由于天气较寒冷且受体牛的饲草料不足,将严重影响胚胎移植妊娠率;8月份供体牛超数排卵的平均采胚数(8.40枚/头)及平均可用胚数均高于12月份的(5.81枚/头),其中平均可用胚数两者差异显著(P<0.05),在天气较寒冷的月份对供体牛进行超数排卵将会影响超排效果;移植双半胚和整胚的妊娠率分别为54.20%(13/24)和27.30%(6/22),前者为后者的2倍,表明在生产实践中通过胚胎分割不但可以成倍增加胚胎的数量,而且可以提高移植后的妊娠率;性别鉴定雌性胚胎冻胚的移植妊娠率40%(40/100),略低于常规冻胚的43.55%(27/62),但差异不显著(P>0.05);2003-2004年全省共移植牛胚胎1208枚,妊娠463头,移植妊娠率37.58%(454/1208)。初步建成了牛良种繁育体系,成效明显。 相似文献
6.
《黑龙江动物繁殖》2015,(5)
2014年在新疆哈密地区伊吾县喀尔里克公司进行绵羊鲜胚移植。受体羊采用两次注射PG法同期发情处理本地哈萨克母羊460只,发情389只,同期发情率为84.5%。供体羊采用PG与阴道栓埋置法同期发情处理,结合FSH递减法超排处理。结果显示:79只供体羊冲出可用胚胎485枚,冷冻胚胎42枚,移植可用胚胎443枚,移植受体羊375只,移植A级胚胎302枚,B、C级胚胎141枚。通过移植,A级胚胎利用率与受胎率明显高于B、C级胚胎,差异均显著(P0.05);71只羊采用PG法,8只羊CIDR法同期处理超排可用胚胎6.24枚与5.25枚,差异不显著(P0.05);供体羊平均超排可用胚胎6.1枚,移植受胎率达76.2%,羔羊成活率为95%。 相似文献
7.
①弥勒红骨山羊两次超排间排卵数、回收胚胎数、回收率、可用胚数、可用胚率均无显著性差异(P＞0.05),但第2次超排后的排卵数、回收胚胎数、可用胚胎数的标准差较大,说明供体羊第2次超排效果均匀度较第1次低.②放牧+补饲和全舍饲两种养殖模式奶山羊同期发情率无显著差异(94.12％vs 88.89％)(P＞0.05),但发情受体的利用率差异显著(81.25％vs 56.25％) (P＜0.05).③放牧+补饲条件下饲养的奶山羊和圭山山羊同期发情率无显著差异(94.12％vs 84.85％) (P＞0.05),但圭山山羊发情受体的利用率较奶山羊低(57.14％vs 81.25％),差异显著(P＜0.05).④移植单胚的圭山山羊和奶山羊妊娠率、产羔率、胚胎获得羔羊的效率无明显差别,移植1～3枚胚胎的奶山羊受体,随移植胚胎数的增加,受体妊娠率逐步提高,从单胚的58.33％提高到双胚的65.22％和三胚的88.89％,母羊产羔率也显著提高,但胚胎获得羔羊的效率无明显差别. 相似文献
8.
本试验对56头娟姗牛与179头荷斯坦青年母牛进行超排处理,分别获得可用胚胎227枚和1242枚;可用胚率分别为57.80%和70.65%,娟姗牛和荷斯坦牛超排黄体数无明显差异(P>0.05),但采胚数明显低于荷斯坦牛,娟姗牛的超排结果明显低于荷斯坦牛的结果(P<0.05),移植受体牛270头,怀孕157头。移植妊娠率分别达到52.54%和50.20%,同期娟姗牛胚胎与荷斯坦牛胚胎移植结果比较,无显著差异(P>0.05)。娟姗牛超排使用FSH剂量为9mg更为经济。青年荷斯坦牛和娟姗牛可用于胚胎生产,但青年娟姗牛的可用胚胎数低于荷斯坦牛。 相似文献
9.
奶牛超数排卵及胚胎移植的试验研究 总被引：1，自引：0，他引：1
本试验采用FSH＋PG的方法对22头供体荷斯坦牛分两批进行了超数排卵处理,140头受体南阳黄牛和供体牛采用2次PG法分批进行了同期发情处理。结果如下：（1）共有20头供体牛、104头受体牛在第二次注射PG后96h内发情,同期发情率分别为90.90%和74.29%;（2）20头供体牛回收胚胎176枚,可用胚胎140枚/头,平均回收胚胎8.8枚/头,平均可用胚胎7.0枚/头,可用胚胎比率79.55%;（3）104头南阳黄牛受体均移植单胚,51头妊娠,妊娠率为49.04%。 相似文献
10.
萨福克羊的胚胎移植初探 总被引：1，自引：0，他引：1
在库车县肉羊繁育场共超排处理萨福克母羊33只,超排有效30只,超排有效率90.9%;获取黄体数361个、回收卵数317枚、回收率87.8%;可用胚胎159枚,可用率为50.2%,末受精卵152枚,可用胚中A、B、C级胚胎分别为102枚、37枚、20枚。比例为64.2%、23.2%、12.6%。供移植受体134只,其中移A、B、C级胚胎移植羊分别为96只、24只、14只;妊娠羊为53只、12只、5只;妊娠率分别为55.2%、50%、35.7%。134只受体中87只多浪羊,47只卡拉库尔羊;35～45dB超检查70只妊娠,其中48只多浪羊、22只卡拉库尔羊;两种受体妊娠率分别为54.3%、45.7%;64只末孕,总妊娠率为52.2%。对250只受体分9批进行同期发情处理,平均同期发情率为89.8%。70只妊娠羊在饲养过程中发现3只有流产迹象,产后死亡2只,计产活羔65只。 相似文献
11.
T C Grahn M L Fahning R Zemjanis 《Journal of the American Veterinary Medical Association》1984,185(4):429-432
A 2-part study was undertaken to determine the effect of bovine viral diarrhea (BVD) virus on fertilization and early development of embryos. In experiment 1, 10 seronegative cows were superovulated and artificially inseminated twice during estrus. After the second insemination, 5 of the cows received intrauterine infusion of BVD virus suspension. The other 5 cows received suspending medium only and served as controls. All 10 cows were slaughtered on day 3, and ova and embryos were collected for morphologic evaluation. A total of 49 and 52 ova and embryos were collected from the control and virus-treated cows, respectively. Among the ova and embryos collected from control cows, 81.6% were fertilized, whereas only 52% were fertilized in the virus-treated group. The statistically significant difference (P less than 0.01) indicated that the virus interferes with fertilization. In experiment 2, the protocol was identical except for slaughter on day 13. Seventy-nine ova and embryos were collected from the 6 control cows, and the 6 virus-treated cows yielded 59 ova and embryos. Of the total ova and embryos recovered on day 13, 88.6% and 50.8% were hatched and developing normally in the control and virus-treated groups, respectively. The difference was highly significant (P less than 0.001). Unfertilized ova and degenerating embryos could not be differentiated on the basis of morphologic appearance. The nearly identical percentages of unfertilized ova in experiment 1 and unhatched ova and embryos in experiment 2 strongly suggested that fertilization failure is the principal manifestation of the observed adverse effect of BVD virus infection. 相似文献
12.
Tahir Karasahin Hasan Alkan Fatma Satilmis Sukru Dursun Huseyin Erdem 《Reproduction in domestic animals》2021,56(12):1555-1561
This study aimed to determine the effect of flunixin meglumine treatment during and after the transfer of in vivo produced embryos to Angus (cows) and Holstein (cows and heifers) breeds of cattle on pregnancy rate. Holstein cows were used as donors in the study. A double dose of prostaglandin F2α was administered to the recipient animals for synchronization. Uterine flushing was performed in donors on day 7 after artificial insemination. A total of 295 transferable embryos were obtained. These embryos were transferred to Angus cows (n = 85), Holstein heifers (n = 80) and Holstein cows (n = 130). After the transfer, these animals were divided into three subgroups. The first subgroup (TI) was administered flunixin meglumine during embryo transfer, and the second subgroup (TII) was administered flunixin meglumine both during embryo transfer and on days 8 and 9 after the transfer. The third subgroup (TIII) was not administered anything and it was considered the control group. Pregnancy examination of the recipients was performed on days 30–35 after the transfer using real-time ultrasonography. The pregnancy rates after embryo transfer were found to be 43.52% in Angus cows, 42.5% in Holstein heifers, and 24.61% in Holstein cows (p < .05). When the animals were not classified according to breed, the pregnancy rates in subgroups TI, TII and TIII were found to be 29.29%, 45.10% and 29.79%, respectively (p < .05). In addition, the pregnancy rates were higher in TII and TIII subgroups of Angus cows and Holstein heifers compared to that of Holstein cows (p < .05). As a result, the pregnancy rates obtained after embryo transfer in Angus cows and Holstein heifers were found to be higher than that in Holstein cows. In addition, it was concluded that the administration of flunixin meglumine during and during/after embryo transfer has a positive effect on pregnancy rates in Angus cows and Holstein heifers. 相似文献
13.
J Ríha 《Veterinární medicína》1984,29(8):457-464
In a trial, 134 donors (cows and first-calvers) culled for slaughter owing to a low performance, udder disease, infertility and other reasons (termination of productive age, foot disease, etc.) were treated with 2000 to 4000 i. u. PMSG (Serum gonadotropin, Bioveta) to induce superovulation. The embryos were obtained post mortem on the sixth to ninth day after the first insemination. Among the donors culled for infertility the proportion of superovulated animals was 53.1% and among those culled for "the other" reasons this proportion was only 46.2%; out of the cows eliminated for a low performance and udder diseases 76.3% and 70.6% were superovulated. The highest stimulation was obtained in cows culled for "the other" reasons (14.58 CL) and for infertility (12.41 CL). The level of embryo gain was good in donors culled for a low performance (57.3% of CL number, 6.82 eggs), for "the other" reasons (59.3%, 8.64 eggs) and for udder diseases (62.6%, 6.83 eggs), it was the lowest in infertile donors (46%; 5.71 eggs-P-insignificant). These donors had the largest proportion of embryos suitable for transfer--66.9% out of the eggs obtained. A good proportion of suitable embryos was also found in donors culled for a low performance (61.7%) and for "the other" reasons (55.8%); the lowest proportion of suitable embryos was obtained in the donors with diseased udders (48.8%). Good proportions of superovulated donors (from 53.5% to 85.7%) and acceptable results of stimulation were kept for about a year after calving.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
14.
Total 240 flushings of superovulated donor cows were examined after double sedimentation. Altogether 2,810 ova and embryos, i.e. average of 11.7 per donor, including 9.0 (76.97%) transferable embryos, were obtained after the first sedimentation. The sedimentation was carried out separately for the respective uterine horns. The second sedimentation, which was carried out in the flushing from both uterine horns simultaneously, yielded ova and embryos in 82.9% (199/240) of the cases. Total 679 ova and embryos were found, i.e. 3.41 per donor, including 2.61 (76.58%) transferable embryos per donor. The increase of the yield of total ova and embryos, and transferable embryos, was 2.82 and 2.16 (24:16 and 24.04%) respectively after the second sedimentation. Altogether 3.489 total ova and embryos were obtained from 240 donors. The average embryo yield was 14.53 per donor with 76.89% (11.17) transferable embryos. The embryo yield/corpora lutea ratio was at the number of corpora lutea 12.04 on the average 120.68%. It is concluded that the second sedimentation of the flushing provides for the release of a part of the embryos for the mucous and cell aggregations, which density is usually lower. The effect of the second sedimentation is considerable, because as much as 24% of the total number of transferable embryos can be saved. The total efficiency of superovulation may be considerably decreased if no attention is paid to the above mentioned facts. 相似文献
15.
Experiments were conducted in 1985 and 1986 at the Eastern Ohio Resource Development Center, Belle Valley, to examine the feasibility of using embryo transfer to induce twinning and to examine the influence of twinning on traits of the cow and calf. Embryos were collected from a total of 14 superovulated Angus donors on two dates each in 1985 and 1986 and were transferred to Angus recipients. A total of 124 embryos were transferred to 79 recipients, with 43 (34.7%) calves born alive. Seven of 45 (15.6%) recipients implanted with two embryos produced twins. In no case did both halves of the 15 embryos that were split to produce identical twins and implanted in the same recipient survive to birth. Proportion of calves born alive did not differ among transfer codes 3 (nonsplit embryos from two different donors implanted in separate uterine horns of the same recipient), 6 (nonsplit embryos from one embryo flush implanted in separate uterine horns of the same recipient) and 7 (nonsplit embryos from two different donors implanted in the same uterine horn of one recipient). Surgical transfers tended to result in a higher proportion of embryos surviving to birth (.43 vs .21; P = .16) and a higher twinning rate (.29 vs .04; P = .36) than did nonsurgical transfers. Age of recipient did not influence embryo survival (P = .98) or twinning rate (P = .99). Gestation length was 5 d shorter (P less than .01) for twin calves than for singles. Singles were 9 kg heavier (P less than .01) at birth and 32 kg heavier (P less than .01) at weaning than twins. However, cows raising twins produced 108 kg (51%) more total weaning weight than did cows raising singles. 相似文献
16.
牛胚胎移植技术在中国内蒙古的研究和应用 总被引：12，自引：2，他引：10
1991－1996年，对6个品种138头供体母牛超排处理199次，共获得A、B级胚胎1148枚，平均每次每头获可用胚5．77枚；共移植受体1299头（包括鲜胚和冻胚），妊娠产犊593头，总移植妊娠率为45．7％。对牛的超数排卵技术进行了深入研究，对不同超排方法的超排效果、不同品种肉牛的超排反应及重复超排的效果进行了分析，获得了适于重复应用、效果稳定的超排方案，邓用FSH－PG法，FSH总剂量在4－6mg,5d 10次递减注射。同时，对受体牛饲养管理条件与受体诱导同期发情效果和移植妊娠率之间的关系进行了探讨，对经产牛和育成牛受本移植妊娠效果进行了比较，并对移植人员技术熟练程度对妊娠率的影响进行了分析，从而为大规模实施牛ET计划提供了受体牛选择依据。 相似文献
17.
Baillargeon P Fecteau G Paré J Lamothe P Sauvé R 《Journal of the American Veterinary Medical Association》2001,218(11):1803-1806
OBJECTIVE: To evaluate efficacy of embryo transfer into seronegative recipients, using the procedure proposed by the International Embryo Transfer Society (IETS), for preventing vertical transmission of Neospora caninum in cattle. DESIGN: Prospective clinical trial. ANIMALS: 87 recipient cows and heifers and their embryo transfer calves from 22 donors originating from 9 dairy herds. PROCEDURE: Neospora caninum serologic status of donors and recipients was determined before collection and transfer of embryos. Viable embryos were washed and treated with trypsin. Recipients in experimental groups A (n = 50) and B (29) were seronegative and received embryos from seropositive and seronegative donors, respectively. Recipients in group C (n = 8) were seropositive and received embryos from seronegative or seropositive donors. Antibody titers against N caninum were determined monthly during pregnancy in recipients and in calf blood samples collected at birth. Tissues collected from stillborn calves and aborted fetuses were analyzed histologically and by immunohistochemical (IHC) methods. RESULTS: 76 calves and 11 fetuses and stillborn calves were examined. All calves from groups A and B were seronegative (n = 70) or lacked evidence of infection by use of tissue analysis (9). In group C, 5 of 6 calves were seropositive at birth, and IHC results were positive for 1 of 2 calves. Vertical transmission rate was significantly lower in groups A and B (0%) than in group C (75%). CONCLUSION AND CLINICAL RELEVANCE: Embryo transfer into seronegative recipients, using the procedure proposed by IETS, is an effective way to prevent vertical transmission of N caninum. Results provide support for pretransfer testing of all embryo transfer recipients. 相似文献
18.
青年荷斯坦奶牛初次超数排卵试验 总被引：4，自引：0，他引：4
对影响荷斯坦青年奶牛超数排卵的因素进行了对比分析,并探索了青年奶牛超排的可行性方案.卵巢上有黄体存在的供体牛可以用于超排.青年奶牛在不同性周期阶段进行超排的结果不同,但差异不显著.用FSH联合氯前列烯醇,采用常规逐日递减法肌肉注射进行超排,平均获得胚胎7.07枚,可用胚胎5.98枚;在人工授精的同时注射促排卵素3号(LRH A3),平均回收胚胎8.64枚,可用胚胎为5.5枚.促排卵素3号能提高胚胎的回收率,但可用胚胎率急剧下降. 相似文献
19.
M Sch?fer T H Tran S Paarmann G Kramer 《Archiv fuer experimentelle veterinaermedizin》1990,44(1):157-162
Blood samples were taken from 58 high-performance cows earmarked as donors for embryo transfer, one day before artificial insemination and following superovulation. An evaluation of 16 major indices showed the yield of transferable embryos to be clearly reduced by hypocholesterolemia, hyperproteinemia, and even more by hypophosphatemia. More than one deviating metabolic findings were obtained only from few animals which had the desired high number of embryos, that is above seven. 相似文献