共查询到20条相似文献，搜索用时 31 毫秒
1.
2.
Effects of methanolic extract and fractions from Litsea cubeba bark on the production of inflammatory mediators in RAW264.7 cells 总被引：4，自引：0，他引：4
Both the methanolic extract and its fractions (0.01 mg/ml) from Litsea cubeba bark were found to inhibit NO and PGE2 production in LPS-activated RAW264.7 macrophages without significant cytotoxicity at less than 0.01 mg/ml concentration. Moreover, L. cubeba bark extract and its fractions significantly decreased the activity of myeloperoxidase catalyzing oxidation of chloride to HOCl and O2- production was reduced by methanol extract (0.05 mg/ml), chloroform fraction (0.025-0.05 mg/ml) and butanol fraction (0.005 mg/ml). These findings suggest that L. cubeba is beneficial for inflammatory conditions and may contain compound(s) with anti-inflammatory properties. 相似文献
3.
Aqueous extract of Vernonia amygdalina infused intragastrically at a dose of 10 mg/ml to rats induced a significant increase in acid output which was reduced by, ranitidine (5 mg/kg b.w.) or atropine (1.2 microMol/kg b.w.). Moreover the extract evoked a dose-dependent contraction of the guinea pig ileum at a dose range of 0.6 mg/ml to 66 mg/ml which was inhibited by atropine (2.4 x 10(-8)-2.4 x 10(-6) M). 相似文献
4.
Determination of hesperetin, cinnamic acid and nicotinic acid in propolis with micellar electrokinetic capillary chromatography 总被引：1，自引：0，他引：1
Micellar electrokinetic capillary chromatography (MEKC) has been used to determine hesperetin, cinnamic acid and nicotinic acid in propolis. After systematically study the effect of buffer concentration and pH, micellar concentration, organic modifier, applied voltage and injection time, the analytical conditions were optimized. Under the optimized conditions, the three analytes could be well separated in 20 min. A good linearity between the peak current and the concentration was found in the range of 0.02-0.60 mg/ml, 0.05-1.20 mg/ml and 0.10-1.40 mg/ml with correlation coefficients of 0.9989, 0.9995 and 0.9990 for hesperetin, cinnamic acid and nicotinic acid, respectively. The detection limits were 0.006 mg/ml (hesperetin), 0.017 mg/ml (cinnamic acid) and 0.036 mg/ml (nicotinic acid). The validity of the method was verified by analyzing the three analytes in propolis oral liquids. 相似文献
5.
Aqueous extracts of the leaf, stem bark and root bark from Canna bidentata, Spondias mombin and Commiphora africana were examined for anthelmintic activity against earthworm. All the extracts demonstrated a concentration-dependent activity at tested concentrations of 10-80 mg/ml. Higher activities were observed at the higher concentrations, 40-80 mg/ml for all the plant extracts. C. bidentata with a paralysis time of 3-5 min and death time of 5-18 min at these concentrations for the stem bark, and S. mombin which exhibited comparatively higher efficacy (34-44 min paralysis time and 105 min death time for the leaf) at lower concentrations of 10-20 mg/ml were adjudged the outstanding anthelmintics of plant origin accordingly. 相似文献
6.
Mitragyna inermis is used in the Senegal traditional medicine for treating stomach and intestinal disorders. At concentrations of 0.5, 0.75 and 1 mg/ml, the aqueous extract of M. inermis bark (AEMIB) significantly induced a decrease of the ileal basal tonus, respectively, from 37+/-1, 51+/-1 and 75+/-2% (P<0.05; n=5), compared to the baseline values. As well as atropine, AEMIB inhibited submaximal contractions induced by 0.01 mg/ml ACh with IC(50) value of approximately 0.75 mg/ml (n=5). These results show that AEMIB possesses both myorelaxant and antispasmodic actions in the ileum. This may justify the pharmacological basis for the popular use of Mitragyna for the treatment of gastrointestinal disorders. 相似文献
7.
The present work was carried out to examine the hypotensive effects of ethanolic extract (EE) from Jatropha gossypiifolia L. The oral administration of EE (125 or 250 mg kg(-1) day(-1)) caused a significant and dose-dependent reduction of the systolic blood pressure. The concentration-response curves to norepinephrine (NE) or Ca(2+) were non-parallelly shifted to the right and the maximum contractile responses were concentration dependently depressed by EE (0.1 or 0.5 mg/ml) in endothelium-deprived mesenteric artery. The cumulative additions of EE (0.1-30 mg/ml) caused a concentration-dependent relaxant response in endothelium-deprived mesenteric artery precontracted with NE or Ca(2+). In conclusion, our results have shown that the EE from J. gossypiifolia L. can elicit hypotension, by oral via, in conscious normotensive rats and vasorelaxant activity on rat mesenteric rings precontracted with NE or Ca(2+). 相似文献
8.
Determination of isoflavones in red clover by capillary electrophoresis with electrochemical detection 总被引：5，自引：0，他引：5
Capillary electrophoresis with electrochemical detection (CE-ED) was employed to analyze isoflavones in red clover (Trifolium pratense). The effects of potential of working electrode, pH and concentration of running buffer, separation voltage and injection time on CE-ED were investigated. Operated in a wall-jet configuration, a 300 microm diameter carbon-disk electrode was used as the working electrode, which exhibits a good response at +0.85 V (versus saturated calomel electrode) for the analytes. The analytes could be separated in a 50 mmol/l borate buffer (pH 9.5) within 25 min. The response was linear over three orders of magnitude with detection limit (S/N=3) ranging from 2x10(-5) mg/ml to 5x10(-5) mg/ml for the analytes. This method has been used for the determination of daidzein, genistein and biochanin A in red clover with satisfactory results. 相似文献
9.
Sangvanich P Kaeothip S Srisomsap C Thiptara P Petsom A Boonmee A Svasti J 《Fitoterapia》2007,78(1):29-31
Crude proteins obtained by Mg/NP-40 extraction from Thai medicinal plants of the Curcuma species exhibited agglutination activity against rabbit erythrocytes. A crude extract from Salingalinthong, a Thai Curcuma specie, exhibited the strongest hemagglutinating activity, 2 x 10(-5) mg/ml. 相似文献
10.
1,7-Dimethoxy-2-hydroxy-xanthone and 1,4-dihydroxy-7-methoxy-xanthone isolated from Securidaca longependunculata were tested for their activity on rabbit corpus cavernosum in vitro. Only the former relaxed the corpus cavernosum by 63% at 1.8 x 10(-5) mg/ml. 相似文献
11.
The oil of the leaves of Bursera aloexylon was found to contain a high linalool level (96.7 %). The antimicrobial activity tests indicated that the oil was effective against Rhodococcus equi (0.60 mg/ml) and Staphylococcus epidermides (0.15 mg/ml). 相似文献
12.
Antioxidant and hepatoprotective effect of Acanthus ilicifolius 总被引：9，自引：0，他引：9
The alcoholic extract of Acanthus ilicifolius leaves inhibited the formation of oxygen derived free radicals (ODFR) in vitro with IC(50) of 550 microg/ml, 2750 microg/ml, 670 microg/ml and 600 microg/ml (Fe(2+)/ascorbate system), 980 microg/ml (Fe(3+)/ADP/ascorbate system) for superoxide radical production, hydroxyl radical generation, nitric oxide radical formation and lipid peroxide formation, respectively. The oral administration of the extract (250 and 500 mg/kg) significantly reduced CCl(4) induced hepatotoxicity in rats, as judged from the serum and tissue activity of marker enzymes [glutamate oxaloacetate transaminase (GOT), glutamate pyruvate transaminase (GPT) and alkaline phosphatase (ALP)]. These results were comparable with those obtained with curcumin (100 mg/kg, p.o.). 相似文献
13.
The antioxidant activity of the methanol extract of Hypericum triquetrifolium Turra (Hypericaceae) aerial part and of flavonoids isolated therein, I3,II8-biapigenin, quercetin-3-O-galactoside, kaempferol-3-O-glycoside, (-)-epicatechin and hypericin, was evaluated. The IC(50) resulted between 0.062 and 1 mg/ml. 相似文献
14.
Yuan Cao Nin-Hua Tan Ji-Jun Chen Guang-Zhi Zeng Yun-Bao Ma Yong-Ping Wu He Yan Jie Yang Lai-Feng Lu Qiang Wang 《Fitoterapia》2010
Three new flavones named 5-carboxymethyl-4′,7-dihydroxyflavone (1), its ethyl ester (2) and butyl ester (3) were isolated from the herb Selaginella moellendorffii Hieron., together with ten known compounds. Their structures were elucidated on the basis of spectroscopic and chemical analysis. Selected compounds were evaluated for their anti-HBV and cytotoxic activity. Among them, compounds 2 and 3 displayed inhibitory activity in vitro on hepatitis B virus (HBV) surface antigen (HBsAg) secretion of the Hep G2.2.15 cell line with IC50 values of 0.17 mg/ml and 0.46 mg/ml, and on HBV e antigen (HBeAg) secretion with IC50 values of 0.42 mg/ml and 0.42 mg/ml, respectively. Compounds 7, 8, 10 and 12 exhibited selective cytotoxicity against the three human cancer cell lines tested. 相似文献
15.
The antitussive activity of Crocus sativus stigma and petal extracts and its components, safranal and crocin, was evaluated using the nebolized solution of citric acid 20% in guinea pigs. The extract and agents were injected intraperitoneally. The ethanolic extract of C. sativus (100-800 mg/kg) and safranal (0.25-0.75 ml/kg) reduced the number of cough. The ethanolic and aqueous extracts of petal and crocin did not show antitussive activity. 相似文献
16.
The present study examined the chemical composition, in vitro antioxidant, anti-hyaluronidase and antifungal activities of essential oils of Melaleuca leucadendron Linn. from Gundih-Central Java, Indonesia in different plant ages of 5, 10 and 15 years old. The Chemical composition of essential oils were analyzed by GC/MS. Twenty-six components were identified, of which 1,8-cineole (49.22–55.04 %), α-terpineol (8.79–10.70 %), d-limonene (5.58–6.39 %), and β-caryophyllene (5.03–7.64 %) were the main compounds in these oils. The antioxidant assay and anti-hyaluronidase assay showed that M. leucadendron leaf oils possess mild antioxidant activity with IC50 between 7.21 and 9.23 mg/ml and anti-hyaluronidase activity with IC50 between 1.94 and 3.03 mg/ml. The antifungal assay showed the effectiveness of these essential oils against Fomitopsis palustris (IC50 0.12–3.16 mg/ml), Trametes versicolor (IC50 0.01–0.06 mg/ml), Cladosporium cladosporioides (IC50 0.03–0.49 mg/ml), and Chaetomium globosum (IC50 0.06–0.15 mg/ml). 相似文献
17.
Extracts from pods and leaves of carob (Ceratonia siliqua L.) were tested for their ability to inhibit cell proliferation of mouse hepatocellular carcinoma cell line (T1). The two extracts showed a marked alteration of T1 cell proliferation in a dose-related fashion reaching the maximal effect at 1 mg/ml. Moreover, we demonstrated that leaf and pod extracts were able to induce apoptosis in T1 cell lines after 24-h treatment mediating a direct activation of the caspase 3 pathway. HPLC analysis revealed the presence of gallic acid, (-) epigallocatechin-3-gallate and (-) epicatechin-3-gallate in pod and leaf extracts, compounds well known to exert antiproliferative effects. Their concentration reached 6.28 mg/g in carob leaves and 1.36 mg/g in carob pods extract. The discovery that carob pod and leaf extracts contained antiproliferative agents could be of practical importance in the development of functional foods and/or chemopreventive drugs. 相似文献
18.
《Fitoterapia》1999,70(3):296-298
The ethanol extract of Melia azedarach ripe fruits showed fungistatic (MIC 50–300 mg/ml) and fungicidal (MFC 60–500 mg/ml) against Aspergillus flavus, Fusarium moniliforme, Microsporum canis and Candida albicans. 相似文献
19.
Insecticidal activity and antifeedant effect of a new type biocide GCSC-BtA and its two reactants Bacillus thuringiensis (B.t.) crystal and Abamectin against third instar larvae of Plutella xylostella L. (Lep., Plutellidae) were investigated using bioassays. GCSC-BtA showed significantly higher toxicity to P. xylostella with LC50 of 0.021 mg/ml than B.t. crystal with 0.060 mg/ml and Abamectin with 0.139 mg/ml in leaf-dip bioassay. Net leaf-dip test
gave LC50s of 0.073, 0.071 and 0.670 mg/ml for GCSC-BtA, B.t. crystal and Abamectin, respectively. The significant differences of the
biocide toxicities between leaf-dip and net leaf-dip methods showed the potential of net leaf-dip method for separating ingestion
and contact poisons. Variation in toxicity of the biocide and two reactants in net leaf-dip method and in mortality in topical
application bioassay showed that GCSC-BtA possessed a combination of strong stomach as well as contact poison property higher
than B.t. or Abamectin. GCSC-BtA indicated no fumigant and systemic toxicities, which the two reactants lacked. In the antifeedant
bioassay, GCSC-BtA acted as a strong antifeedant to the larvae of P. xylostella. Leaf consumption by the larvae after GCSC-BtA treatment was 0.04–0.60 mm2 with a concentration range of 0.04–4.00 mg/ml, which was lower than 0.26–1.02 and 0.04–6.47 mm2 for B.t. crystal and Abamectin, respectively. DC50 (concentration resulting in a deterrence index of 50%) value for GCSC-BtA was 0.089 mg/ml, which was also significantly lower
than 0.275 and 0.736 mg/ml for B.t. crystal and Abamectin, respectively. 相似文献
20.
Onion has had an important dietary and medicinal role for centuries. In this study the antileishmanial effect of aqueous onion extract (AOE) was investigated. Five leishmanial strains in the promastigote stage were studied in vitro. Seventy-two hour inoculation of AOE gave an IC100 and average IC50 values of 1.25 mg/ml and 0.376 mg/ml, respectively, against all leishmanial strains tested. 相似文献