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1.
Since the development of effective N2O mitigation options is a key challenge for future agricultural practice, we studied the interactive effect of tillage systems on fertilizer-derived N2O emissions and the abundance of microbial communities involved in N2O production and reduction. Soil samples from 0–10 cm and 10–20 cm depth of reduced tillage and ploughed plots were incubated with dairy slurry (SL) and manure compost (MC) in comparison with calcium ammonium nitrate (CAN) and an unfertilized control (ZERO) for 42 days. N2O and CO2 fluxes, ammonium, nitrate, dissolved organic C, and functional gene abundances (16S rRNA gene, nirK, nirS, nosZ, bacterial and archaeal amoA) were regularly monitored. Averaged across all soil samples, N2O emissions decreased in the order CAN and SL (CAN?=?748.8?±?206.3, SL?=?489.4?±?107.2 μg kg?1) followed by MC (284.2?±?67.3 μg kg?1) and ZERO (29.1?±?5.9 μg kg?1). Highest cumulative N2O emissions were found in 10–20 cm of the reduced tilled soil in CAN and SL. N2O fluxes were assigned to ammonium as source in CAN and SL and correlated positively to bacterial amoA abundances. Additionally, nosZ abundances correlated negatively to N2O fluxes in the organic fertilizer treatments. Soils showed a gradient in soil organic C, 16S rRNA, nirK, and nosZ with greater amounts in the 0–10 than 10–20 cm layer. Abundances of bacterial and archaeal amoA were higher in reduced tilled soil compared to ploughed soils. The study highlights that tillage system induced biophysicochemical stratification impacts net N2O emissions within the soil profile according to N and C species added during fertilization.  相似文献   

2.
Abstract

To understand the influence of basal application of N fertilizer on nitrification potential and N2O and NO emissions, four soil samples were collected from an upland Andisol field just before (sample 1) and 4 (sample 2), 36 (sample 3) and 72 (sample 4) days after the basal application of N fertilizer during the Chinese cabbage growing season from 12 September to 30 November 2005. The potentials of N2O production and nitrification of the soils were determined using a 15N tracer technique and the soils were incubated for 25 days at 25°C and 60% water-filled pore space (WFPS). The results revealed that as much as 84–97% N2O and almost all NO were produced by nitrification. The 15N2O emission peak occurred approximately 350 h after the beginning of incubation for samples 1 and 2, but just 48 h later in samples 3 and 4. Total 15N2O emission during the 25-day incubation of samples 3 and 4 ranged from 190 to 198 µg N kg?1 soil, which was significantly higher than the 99–108 µg N kg?1 soil recorded in samples 1 and 2. Basal application of N fertilizer did not immediately increase the nitrification potential and the ratio of N2O to N added, but did dramatically increase the nitrification potential and the ratio of N2O to N added as (15NH4)2SO4 36–72 days after the basal N fertilizer was added. In contrast, NO emission was negatively correlated with nitrification potential and total N2O emission. As a result, a trade-off relationship between total NO and N2O emissions was identified. The results indicated that there was a time-lagged induction of the change of N turnover in the soil, which was possibly caused by slow population growth of the nitrifiers and/or a slow shift in the microbial community in the soil.  相似文献   

3.
Relative control of soil moisture [30, 60, and 80 percent water-holding capacity (WHC)] on nitrous oxide (N2O) emissions from Fargo-Ryan soil, treated with urea at 0, 150, and 250 kg N ha?1 with and without nitrapyrin [2-chloro-(6-trichloromethyl) pyridine] (NP), was measured under laboratory condition for 140 days. Soil N2O emissions significantly increased with increasing nitrogen (N) rates and WHC levels. Urea applied at 250 kg N ha?1 produced the greatest cumulative N2O emissions and averaged 560, 3919, and 15894 µg kg?1 at 30, 60, and 80 percent WHC, respectively. At WHC ≤ 60 percent, addition of NP to urea significantly reduced N2O losses by 2.6- to 4.8-fold. Additions of NP to urea reduced N2O emission at rates similar to the control (0 N) until 48 days for 30 percent WHC and 35 days for 60 and 80 percent WHC. These results can help devise urea-N fertilizer management strategies in reducing N2O emissions from silty-clay soils.  相似文献   

4.
Soil moisture changes, arising from seasonal variation or from global climate changes, could influence soil nitrogen (N) transformation rates and N availability in unfertilized subtropical forests. A 15?N dilution study was carried out to investigate the effects of soil moisture change (30–90 % water-holding capacity (WHC)) on potential gross N transformation rates and N2O and NO emissions in two contrasting (broad-leaved vs. coniferous) subtropical forest soils. Gross N mineralization rates were more sensitive to soil moisture change than gross NH4 + immobilization rates for both forest soils. Gross nitrification rates gradually increased with increasing soil moisture in both forest soils. Thus, enhanced N availability at higher soil moisture values was attributed to increasing gross N mineralization and nitrification rates over the immobilization rate. The natural N enrichment in humid subtropical forest soils may partially be due to fast N mineralization and nitrification under relatively higher soil moisture. In broad-leaved forest soil, the high N2O and NO emissions occurred at 30 % WHC, while the reverse was true in coniferous forest soil. Therefore, we propose that there are different mechanisms regulating N2O and NO emissions between broad-leaved and coniferous forest soils. In coniferous forest soil, nitrification may be the primary process responsible for N2O and NO emissions, while in broad-leaved forest soil, N2O and NO emissions may originate from the denitrification process.  相似文献   

5.
Molecular nitrogen (N2) and nitrous oxide (N2O) generated by denitrification increase N losses in the soil–plant system. This study aimed to quantify N2 and N2O from potassium nitrate (K15NO3) applied to soils with different textures and moisture contents in the absence and presence of a source of carbon (C) using the 15N tracer method. In the three soils used (sandy texture (ST), sandy clay loam texture (SCLT), and clayey texture (CT)), three moisture contents were evaluated (40%, 60%, and 80% of the water holding capacity (WHC)) with (D+) and without (D?) dextrose added. The treatments received 100 mg N kg?1 (KNO3 with 23.24 atom% 15N). N2 emissions occurred in all of the treatments, but N2O emissions only occurred in the D+ treatment, showing increases with increasing moisture content. SCLT with 80% WHC in the D+ treatment exhibited the highest accumulated N emission (48.26 mg kg?1). The 15N balance suggested trapping of the gases in the soil.  相似文献   

6.
Quantitative measurements of plant growth characteristics, forage production, nitrogen (N) fixation, and soil N accumulation by white clover were determined in a field experiment at the subhumid hilly region of Rawalakot, Azad Jammu and Kashmir (AJK). Three indigenous and two exotic ecotypes of white clover were used in the study. Indigenous ecotypes were collected from three different locations (i.e., Tollipir, Banjosa, and Rawalakot), whereas exotic ecotypes (NuSiral and Irrigation) were collected from New South Wales Agricultural Research and Advisory Station, Australia. Data were collected for two seasons (spring 2004–autumn 2004). Total average values for height, number of stolons, length of stolons, number of leaves, and leaf area were 13–50 cm, 9–20, 2–4 cm, 23–81, and 7–16 cm2, respectively. The morphological characteristics of exotic ecotypes were significantly higher than the indigenous ecotypes, and the percentage increase in different plant characteristics was +6% to 214%. Total herbage dry‐matter yield (DMY) in the indigenous and exotic ecotypes varied between 0.5–2.3 and 3.6–4 Mg ha?1, respectively. All the ecotypes showed substantial nodulation potential, and the number of nodules in plant roots ranged from 65 to 119, confirming the presence of indigenous Rhizobium population in the soil. The N contents of harvested herbage of white clover were 2.3–3.0% compared to 0.85% in the grass, and the estimated rates of N2 fixation were 26 kg N ha?1 in the indigenous to 79 kg N ha?1 in the exotic ecotypes. Amount of N2 fixed was strongly correlated with DMY, suggesting that crop DM can be used as an indicator of N2 fixation in white clover. Protein content of white clover was 14–19%, compared to 5% in the indigenous grass species. Total organic carbon (C) and N in control soil were 8.5 and 0.75 g kg?1, which increased significantly to 13.1 and 0.93 g kg?1 in soil under white clover. It is concluded that white clover has substantial potential for growth and establishment in the subhumid hilly regions and can be used to recuperate degraded soils because of its ability to sustain high level of pasture production and increase the N status of soil. These benefits could be of particular use for small‐scale resource‐poor farmers.  相似文献   

7.
Soil microbes are frequently limited by carbon (C), but also have a high phosphorus (P) requirement. Little is known about the effect of P availability relative to the availability of C on soil microbial activity. In two separate experiments, we assessed the effect of P addition (20 mg P kg?1 soil) with and without glucose addition (500 mg C kg?1 soil) on gross nitrogen (N) mineralization (15N pool dilution method), microbial respiration, and nitrous oxide (N2O) emission in a grassland soil. In the first experiment, soils were incubated for 13 days at 90% water holding capacity (WHC) with addition of NO3? (99 mg N kg?1 soil) to support denitrification. Addition of C and P had no effect on gross N mineralization. Initially, N2O emission significantly increased with glucose, but it decreased at later stages of the incubation, suggesting a shift from C to NO3? limitation of denitrifiers. P addition increased the N2O/CO2 ratio without glucose but decreased it with glucose addition. Furthermore, the 15N recovery was lowest with glucose and without P addition, suggesting a glucose by P interaction on the denitrifying community. In the second experiment, soils were incubated for 2 days at 75% WHC without N addition. Glucose addition increased soil 15N recovery, but had no effect on gross N mineralization. Possibly, glucose addition increased short-term microbial N immobilization, thereby reducing N-substrates for nitrification and denitrification under more aerobic conditions. Our results indicate that both C and P affect N transformations in this grassland soil.  相似文献   

8.
A better understanding of the nitrogen (N) cycle in agricultural soils is crucial for developing sustainable and environmentally friendly N fertilizer management and to propose effective nitrous oxide (N2O) mitigation strategies. This laboratory study quantified gross nitrogen transformation rates in uncultivated and cultivated black soils in Northeast China. It also elucidated the contribution made by nitrification and denitrification to the emissions of N2O. In the laboratory, soil samples adjusted to 60 % water holding capacity (WHC) were spiked with 15NH4NO3 and NH4 15NO3 and incubated at 25 °C for 7 days. The size and 15N enrichment of the mineral N pools and the N2O emission rates were determined between 0 and 7 days. The results showed that the average N2O emission rate was 21.6 ng N2O-N kg?1 h?1 in cultivated soil, significantly higher than in the uncultivated soil (11.6 ng N2O-N kg?1 h?1). Denitrification was found to be responsible for 32.1 % of the N2O emission in uncultivated soil, and the ratio increased significantly to 43.2 % in cultivated soil, due to the decrease in soil pH. Most of the increase in net N2O-N emissions observed in the cultivated soil was resulting from the increased production of N2O through denitrification. Gross nitrification rate was significantly higher in the cultivated soil than in the uncultivated soil, and the ratio of gross nitrification rate/ammonium immobilization rate was 6.87 in cultivated soil, much larger than the uncultivated soil, indicating that nitrification was the dominant NH4 + consuming process in cultivated soil, and this will lead to the increased production of nitrate, whereas the increased contribution of denitrification to N2O emission promoted the larger emission of N2O. This double impact explains why the risk of N loss to the environment is increased by long-term cultivation and fertilization of native prairie sites, and controlling nitrification maybe effective to abate the negative environmental effects.  相似文献   

9.

Purpose

The effects of soil pH manipulation and KCl addition on N2O production in adjacent forest and grassland soils in central Alberta were studied in a 16-day laboratory incubation experiment.

Materials and methods

The soils were subjected to four pH and two salt treatments: CK (control)—no addition of acid or alkali solution (pH 4.50 and 4.48 for the forest and grassland soils, respectively; same below); HCl—addition of HCl solution to lower soil pH (3.95 and 3.75); L-KOH and H-KOH—addition of 6 mL of 0.2 (5.36 and 5.57) and 0.4 (6.41 and 6.72)?mol?L?1 KOH solution, respectively, to increase soil pH to two different levels. In order to differentiate between the effect of a change in pH and of changed salt concentrations on N2O production, 6 mL of 0.2 (L-KCl) (4.56 and 4.41) or 0.4 mol?L?1 (H-KCl) (4.59 and 4.42) KCl solutions were also applied as treatments to create two levels of salt application rates.

Results and discussion

Increasing pH promoted gross nitrification and cumulative N2O production in both soils, particularly in the forest soil. However, cumulative N2O production decreased in the forest soil but increased in the grassland soil when pH decreased. Cumulative N2O production in the grassland soil was 36 times higher in the L-KCl treatment (1,442 μg?N?kg?1) than in the CK (40 μg?N?kg?1), whereas the H-KCl treatment reduced cumulative N2O production. In contrast, in the forest soil, both KCl treatments reduced cumulative N2O production.

Conclusions

(1) The most important factor to increase N2O production in this study was increasing soil pH, suggesting that careful soil pH management could be used as a tool to control soil N2O production; (2) salt effect was also involved in affecting N2O production.  相似文献   

10.
A 56-day aerobic incubation experiment was performed with 15-nitrogen (N) tracer techniques after application of wheat straw to investigate nitrate-N (NO3-N) immobilization in a typical intensively managed calcareous Fluvaquent soil. The dynamics of concentration and isotopic abundance of soil N pools and nitrous oxide (N2O) emission were determined. As the amount of straw increased, the concentration and isotopic abundance of total soil organic N and newly formed labeled particulate organic matter (POM-N) increased while NO3-N decreased. When 15NO3-N was applied combined with a large amount of straw at 5000 mg carbon (C) kg?1 only 1.1 ± 0.4 mg kg?1 NO3-N remained on day 56. The soil microbial biomass N (SMBN) concentration and newly formed labeled SMBN increased significantly (P < 0.05) with increasing amount of straw. Total N2O-N emissions were at levels of only micrograms kg?1 soil. The results indicate that application of straw can promote the immobilization of excessive nitrate with little emission of N2O.  相似文献   

11.
Abstract

We studied the effect of crop residues with various C:N ratios on N2O emissions from soil. We set up five experimental plots with four types of crop residues, onion leaf (OL), soybean stem and leaf (SSL), rice straw (RS) and wheat straw (WS), and no residue (NR) on Gray Lowland soil in Mikasa, Hokkaido, Japan. The C:N ratios of these crop residues were 11.6, 14.5, 62.3, and 110, respectively. Based on the results of a questionnaire survey of farmer practices, we determined appropriate application rates: 108, 168, 110, 141 and 0 g C m?2 and 9.3, 11.6, 1.76, 1.28 and 0 g N m?2, respectively. We measured N2O, CO2 and NO fluxes using a closed chamber method. At the same time, we measured soil temperature at a depth of 5 cm, water-filled pore space (WFPS), and the concentrations of soil NH+ 4-N, NO? 3-N and water-soluble organic carbon (WSOC). Significant peaks of N2O and CO2 emissions came from OL and SSL just after application, but there were no emissions from RS, WS or NR. There was a significant relationship between N2O and CO2 emissions in each treatment except WS, and correlations between CO2 flux and temperature in RS, soil NH+ 4-N and N2O flux in SSL and NR, soil NH+ 4-N and CO2 flux in SSL, and WSOC and CO2 flux in WS. The ratio of N2O-N/NO-N increased to approximately 100 in OL and SSL as N2O emissions increased. Cumulative N2O and CO2 emissions increased as the C:N ratio decreased, but not significantly. The ratio of N2O emission to applied N ranged from ?0.43% to 0.86%, and was significantly correlated with C:N ratio (y = ?0.59 ln [x] + 2.30, r 2 = 0.99, P < 0.01). The ratio of CO2 emissions to applied C ranged from ?5.8% to 45% and was also correlated with C:N ratio, but not significantly (r 2 = 0.78, P = 0.11).  相似文献   

12.
Yak and Tibetan sheep graze extensively on natural grasslands in the Qinghai-Tibetan Plateau, and large amounts of excrement are directly deposited onto alpine grasslands. However, information on greenhouse gas (GHG) emissions from this excrement is limited. This study evaluated the short-term effects of yak and Tibetan sheep dung on nitrous oxide (N2O), methane (CH4), and carbon dioxide (CO2) emissions from alpine steppe soil at a water holding capacity (WHC) of 40 or 60 % and from alpine meadow soil at a WHC of 60 or 80 % under laboratory conditions. Cumulative N2O emissions over a 15-day incubation period at low soil moisture conditions ranged from 111 to 232 μg N2O–N kg soil?1 in the yak dung treatments, significantly (P?<?0.01) higher than that of sheep dung treatments (28.7 to 33.7 μg N2O–N kg soil?1) and untreated soils (1.04–6.94 μg N2O–N kg soil?1). At high soil moisture conditions, N2O emissions were higher from sheep dung than yak dung and non-treated soils. No significant difference was found between the yak dung and non-treated alpine meadow soil at 80 % WHC. Low N2O emission in the yak dung treatment from relatively wet soil was probably due to complete denitrification to N2. Yak dung markedly (P?<?0.001) increased CH4 and CO2 emissions, likely being the main source of these two gases. The addition of sheep dung markedly (P?<?0.001) elevated CO2 emissions. Dung application significantly (P?<?0.01) increased global warming potential, particularly for alpine steppe soil. In conclusion, our findings suggest that yak and Tibetan sheep dung deposited on alpine grassland soils may increase GHG emissions.  相似文献   

13.
The potential of Nostoc 9v for improving the nitrogen (N)2–fixing capacity and nutrient status of semi‐arid soils from Tanzania, Zimbabwe, and South Africa was studied in a laboratory experiment. Nostoc 9v was inoculated on nonsterilized and sterilized soils. Inoculum rates were 2.5 mg dry biomass g?1 soil and 5 mg dry biomass g?1 soil. The soils were incubated for 3 months at 27 °C under 22 W m2 illumination with a photoperiod of 16 h light and 8 h dark. The moisture was maintained at 60% of field capacity. In all soils, Nostoc 9v proliferated and colonized the soil surfaces very quickly and was tolerant to acidity and low nutrient availability. Cyanobacteria promoted soil N2 fixation and had a pronounced effect on total soil organic carbon (SOC), which increased by 30–100%. Total N also increased, but the enrichment was, in most soils, comparatively lower than for carbon (C). Nitrate and ammonium concentrations, in contrast, decreased in all the soils studied. Increases in the concentration of available macronutrients were produced in most soils and treatments, ranging from 3 to 20 mg phosphorus (P) kg?1 soil, from 5 to 58 mg potassium (K) kg?1 soil, from 4 to 285 mg calcium (Ca) kg?1, and from 12 to 90 mg magnesium (Mg) kg?1 soil. Positive effects on the levels of available manganese (Mn) and zinc (Zn) were also observed.  相似文献   

14.

Purpose

In view that soils are bodies and that processes such as storage and release of water, carbon, nutrients and pollutants, and aeration and rooting happen in these bodies, it is of interest to know the density of elements and compounds in soils. On the basis of soil bulk and element density of organic carbon (OC), N, and heavy metals in soils and of horizon thickness, stocks of these elements for garden soils were calculated.

Materials and methods

Fourteen gardens in four allotments of the northwestern part of the Ruhr area, Germany were investigated. The research included 14 vegetable patches, 13 lawns, 2 compost heaps, and 1 meadow. Volume samples were taken. The soil analysis included pH, soil bulk density, and OC, N, Pb, Cd, Zn, Cu, and Ni contents.

Results and discussion

The soils were from sandy loam to loamy sand. The pH was slightly acid and C/N ratio about 20. Soil bulk density was between 0.8 and 1.4 g cm?3 and mean bulk density was 1.1 g cm?3. Mean OC content was for compost 7.4 %, vegetable patches 5.2 % (0–30 cm depth), and lawns and meadow 5.8 and 5.2 % (0–5 cm depth). OC density for compost was 76 mg cm?3, vegetable patches 56 mg cm?3, and lawns 67 mg cm?3 (0–5 cm). Mean OC stock in 0–30 cm soil depth in vegetable patches was 16.4 kg m?2, lawns 15.5 kg m?2, and meadow 11.1 kg m?2. N contents were between 0.06 and 0.46 %. For compost, the mean was 0.39 %, vegetable patches 0.27 % (0–30 cm), lawn 0.28 %, and meadow 0.26 % (0–5 cm). Mean stock of N in 0–30 cm depth for vegetable patches was 0.84 kg m?2, lawn 0.76 kg m?2, and meadow 0.55 kg m?2. For heavy metals in compost, vegetable patches, lawn and meadow, Cd contents were in the range of 1.7 to 3.0 mg kg?1, Pb 49 to 152 mg kg?1, and Zn 52 to 1830 mg kg?1. The amounts stored per square meters in 30 cm depth were for Cd 0.6–1.1 g, Pb 15–52 g, Zn 41–440 g, Cu 4–39 g, and Ni 1–8 g.

Conclusions

Allotment gardens have a high capacity to store CO2 as OC. Roughly, there will be 7–8 million tons of OC stored in the 1.3 million allotment gardens of Germany. The high amount of 8000 kg N ha?1 could damage the groundwater when released by wrong soil management. Cd, Zn, Pb, Cu, and Ni amounts of 7.8, 1000, 300, 135, and 30 kg ha?1, respectively, are a lasting burden.
  相似文献   

15.
The objective of this study was to adapt the partial chemical digestion method for estimation of labile soil organic matter pools by evaluating the effect of different digestion times in Mollisols of the Argentine Pampas. The soils were sampled from nine agricultural fields under no-tillage at the 0–20 cm depth. A chemical method was performed through partial soil digestion with dilute sulphuric acid at 100°C on the basis of four digestion times: 1 (Nd1), 2 (Nd2), 4 (Nd4) and 6 (Nd6) hours. Soil organic carbon (C) and nitrogen (N) fractions were determined. The extracted organic N (Nd) ranged from 0.076 g kg?1 to 0.273 g kg?1, with a mean of 0.154 g kg?1. Statistically, the means for each digestion time indicated highly significant differences (P = 0.008). High correlations were found between Nd for different times and labile C and N fractions. However, the best fit prediction was observed between Nd2 and soil total carbohydrates (CHt), with a high coefficient of determination (R2 = 0.94). Partial chemical digestion for 2 h can be used as a rapid indicator to accurately predict CHt. Because of its speed and simplicity, this method may also be useful for rapid soil quality assessments.  相似文献   

16.
Abstract

To investigate the effects of renovation (ploughing and resowing) on nitrous oxide (N2O) emissions from grassland soil, we measured N2O fluxes from renovated and unrenovated (control) grassland plots. On 22 August in both 2005 and 2006 we harvested the sward, ploughed the surface soil and then mixed roots and stubble into the surface soil with a rotovator. Next, we compacted the soil surface with a land roller, spread fertilizer at 40 kg N ha?1 on the soil surface and sowed orchardgrass (Dactylis glomerata L., Natsumidori). In the control plot, we just harvested the sward and spread fertilizer. We determined N2O fluxes for 2 months after the renovation using a vented closed chamber. During the first 2 weeks, the renovated plot produced much more N2O than the control plot, suggesting that N was quickly mineralized from the incorporated roots and stubble. Even after 2 weeks, however, large N2O emissions from the renovated plot were recorded after rainfall, when the soil surface was warmed by sunshine and the soil temperature rose 2.7–3.0°C more than that of the control plot. In 2005, during the 67-day period from 19 August to 26 October, the renovated and control plots emitted 5.3 ± 1.4 and 2.8 ± 0.7 kg N2O-N ha?1, with maximum fluxes of 3,659 and 1,322 µg N2O-N m?2 h?1, respectively. In 2006, during the 65-day period from 21 August to 26 October, the renovated and control plots emitted 2.1 ± 0.6 and 0.96 ± 0.42 kg N2O-N ha?1, with maximum fluxes of 706 and 175 µg N2O-N m?2 h?1, respectively. The cumulative N2O emissions from plots in 2005 were greater than those in 2006, presumably because rainfall just after renovation was greater in 2005 than in 2006. These results suggest that incorporated roots and stubble may enlarge the anaerobic microsites in the soil in its decomposing process and increase the N2O production derived from the residues and the fertilizer. In addition, rainfall and soil moisture and temperature conditions during and after renovation may control the cumulative N2O emission.  相似文献   

17.
利用15N同位素标记方法,研究在两种水分条件即60％和90％ WHC下,添加硝酸盐(NH4NO3,N 300 mg kg-1)和亚硝酸盐(NaNO2,N 1 mg kg-1)对中亚热带天然森林土壤N2O和NO产生过程及途径的影响.结果表明,在含水量为60％ WHC的情况下,高氮输入显著抑制了N2O和NO的产生(p＜0.01);但当含水量增为90％ WHC后,实验9h内抑制N2O产生,之后转为促进.所有未灭菌处理在添加NO2-后高氮抑制均立即解除并大量产生N2O和NO,与对照成显著差异(p＜0.01),在60％ WHC条件下,这种情况维持时间较短(21 h),但如果含水量高(90％ WHC)这种情况会持续很长时间(2周以上),说明水分有效性的提高和外源NO2-在高氮抑制解除中起到重要作用.本实验中N2O主要来源于土壤反硝化过程,而且加入未标记NO2-后导致杂合的N2O(14N15NO)分子在实验21 h内迅速增加,表明这种森林土壤的反硝化过程可能主要是通过真菌的“共脱氮”来实现,其贡献率可多达80％以上.Spearman秩相关分析表明未灭菌土壤NO的产生速率与N2O产生速率成显著正相关性(p＜0.05),土壤含水量越低二者相关性越高.灭菌土壤添加NO2-能较未灭菌土壤产生更多的NO,但却几乎不产生N2O,表明酸性土壤的化学反硝化对NO的贡献要大于N2O.  相似文献   

18.
Soil cultivation changes and usage of agricultural wastes can have profound impacts on greenhouse gas (GHG) emission from soil. In this study, the effects of soil cultivation and organic amendment on GHG emission were investigated using aerobic incubation. Surface soil (0–20 cm) from (1) rice–legume consecutive rotation (Rice) and (2) recently (<3 years) converted from rice field to plastic-covered intensive vegetable and flower production (VegC) were collected in Kunming, P.R. China. Rose (Rosa rugosa Thunb.) residues and cattle manure were applied at 5% by weight. Results indicated that N2O and CO2 fluxes were significantly influenced by soil cultivation, organic amendment, incubation time and their interaction (p <0.05). Applying cattle manure increased, while rose residue decreased, cumulative N2O emissions from soil (84 days). Rose residue application significantly increased cumulative CO2 emissions with peak values of 6371 (Rice) and 7481 mg kg?1 (VegC), followed by cattle manure addition figure of 2265 (VegC) and 3581 mg kg?1 (Rice). Both were significantly higher (p <0.05) than the un-amended Control at 709 (VegC) and 904 mg kg?1 (Rice). Our study demonstrates that a low C/N ratio in cattle manure is better than a high C/N ratio in rose residue in regard to reducing the global warming potential of agricultural soil.  相似文献   

19.
Available iron, zinc, copper and manganese were determined in six pedons located in upper slope, middle slope and valley bottom soils derived from Abeokuta geological materials in Nigeria. The soils had an average of 639.8 g kg?1 sand, 241.8 g kg?1 clay and 118.4 g kg?1 silt. The fertility status of the soils was low–medium with a strongly acid–neutral reaction, 1.3–15.1 g kg?1 organic carbon contents, moderate–high exchangeable bases and 1.38 mg kg?1 available phosphorus. Both Fe (122.50 mg kg?1) and Mn (111.40 mg kg?1) occurred at toxic levels, whereas the mean Cu (1.27 mg kg?1) and Zn (2.56 mg kg?1) contents were found to be adequate for most crops grown in the region. There were significant positive correlations among the micronutrients and also between soil pH, organic carbon, particle size fractions and micronutrients. The high levels of Fe and Mn were probably due to the presence of oolitic ironstone in the parent material.  相似文献   

20.
Quantification of soil carbon (C) and nitrogen (N) fractions in grasslands is vital for estimating C sequestration and climate change studies. We quantified background soil total carbon (TOC) status, recalcitrant carbon (RC), acid hydrolysable labile carbon (AHC), hot- and cold-water extractable carbon (HWC and CWC, respectively) fractions in the grassland in this study. Soil C fractions were as follows: TOC (11,633 to 15,525 mg C kg?1); RC (11,500 to 15,357 mg C kg?1); AHC (132 to 168 mg C kg?1); HWC (57 to 70 mg C kg?1); and CWC (27 to 33 mg C kg?1). Labile C fractions contributed at most 1.2% to total C. Concentrations of total N ranged from 1072 to 1230 g N kg?1. Recalcitrant C contributed higher amounts (>90%) to total C, indicating the incorporation of C into the stable C fraction. Carbon dioxide (CO2) and nitrous oxide (N2O) fluxes were not significantly correlated with total C.  相似文献   

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