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Jakab C Halász J Szász AM Batmunkh E Kiss A Schaff Z Rusvai M Gálfi P Kulka J 《Acta veterinaria Hungarica》2008,56(3):341-352
The recently identified claudins are dominant components of tight junctions, responsible for cell adhesion, polarity and paracellular permeability. Certain claudins have been shown to have relevance in tumour development. The aim of the present study was to analyse the expression of claudin-1, -2, -3, -4, -5, -7 and -10 in normal canine mammary glands. Samples from the inguinal mammary regions of 20 non-castrated, 1-13 years old female dogs were studied. Immunohistochemical analysis was performed on conventional specimens and tissue microarrays. The results of the immunohistochemical reactions detecting claudins in tissue sections were photodocumented. The immunoreactivity of claudins was quantitatively analysed on digital images using Leica QWin morphometry software. Intense membranous immunolabelling was found for claudin-1, -3 and -7, intense membranous with non-granular cytoplasmic immunolabelling for claudin-2, moderate membranous immunolabelling for claudin-4 and -5, and weak membranous immunolabelling for claudin-10. The occurrence of tight junctions was confirmed by ultrathin section electron microscopy. The available data suggested that claudins might be proteins preserved throughout the evolution of mammals. The results of our study support the concept that they are indeed preserved, since the same type of claudins, in identical distribution, could be detected in our canine mammary tissue samples as could be found in human mammary tissue. 相似文献
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啤酒酵母中(1-3)-β-D-葡聚糖提取与理化性能分析 总被引：13，自引：0，他引：13
(1-3)-β-D-葡聚糖是一种极富生物活性的多糖，它表现出很强的刺激免疫和抗肿瘤活性，是一种良好的生物反应调节剂．在啤酒酵母细胞壁中，含有大量的(1-3)-β-D-葡聚糖，我国的酵母资源十分丰富，估计年产啤酒酵母3～5万吨(干基)。可是我国啤酒制造业对啤酒废酵母没有很好的重视与利用。啤酒废酵母作为废弃物排人下水道或以低廉的价格售作饲料，造成环境污染和资源浪费。因此酵母提取物的研究与开发具有重要意义。 相似文献
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褪黑素的英文名为Melatonin,学名N—乙酰基—5—甲氧基色胺,分子式C_(13)H_(16)N_2O_2。褪黑素是由松果体分泌的一种激素,具有调节睡眠、延缓衰老、增强免疫、抑制肿瘤、预防心血管疾病及其它多种调节功能。1 褪黑素与睡眼 褪黑素与睡眠周期有着密切关系。研究发现,褪黑激素在一天24小时中并不是均匀分泌的,而是有昼少夜多的规律。具体数据见表1。 表1 30岁健康成人一昼夜的血清褪黑素含量变化 相似文献
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Ribeiro L. A. Turba M. E. Bernardini C. Zannoni A. Bacci M. L. Forni M. 《Veterinary research communications》2007,31(1):193-196
Veterinary Research Communications - Ribeiro, L.A., Turba, M.E., Bernardini, C., Zannoni, A., Bacci, M.L. and Forni, M., 2007. Matrix metalloproteinases -2 and -9 in swine luteal tissue... 相似文献
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为获得增强型绿色荧光蛋白(Enhanced green fluorescent protein,EGFP)的真核表达载体pEFP-N1-MSC,并观察其在猪成纤维细胞中的表达情况,用Nhe Ⅰ和Age Ⅰ消除pEGFP-N1质粒上的多克隆位点(MSC),然后补平连接,获得增强型绿色荧光蛋白编码基因的真核表达载体pEGFP-N1-MSC质粒,将pEGFP-N1-MSC质粒转化DH5α感受态细胞,于Kanr LB平板上筛选阳性克隆.重组子经Nhe Ⅰ和Age Ⅰ双酶切鉴定,将该载体转染猪成纤维细胞,24 h后观察EGFP表达情况,48 h后添加G418进行筛选.结果表明:改造的pEGFP-N1-MSC真核表达载体,成功转染猪成纤维细胞,在倒置荧光显微镜下呈现绿色光,获得可产生绿色荧光的pEGFP-N1-MSC载体,为构建猪双正选择同源重组载体奠定了基础. 相似文献
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ＺＪＨ９２－１型和ＺＪＨ９２－２型烘茧灶是浙７３－１型烘茧灶的改进灶型。文中对新旧灶型的性能和蚕茧干燥质量进行了较全面的测试。测试数据表明，用新灶型替代老灶型，经济效益明显提高。 相似文献
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南金花 《青海畜牧兽医杂志》2012,42(3):9-10
为了探讨口蹄疫O型-亚洲Ⅰ型二价灭活苗免疫接种牦牛、藏羊后产生抗体效价,项目对天峻县阳康乡的牦牛、藏羊开展AsiaⅠ-O型口蹄疫疫苗的抗体效价检测,试验结果表明,口蹄疫AsiaⅠ-O型二价灭活疫苗免疫效果依次为成年牦牛、成年藏羊、犊牛、羔羊;免疫后Ⅰ型抗体分析结果与O型免疫抗体的免疫效果一致,免疫合格率在80%以上,免疫牛羊产生的整体抗体水平能够符合国家规定的要求。 相似文献
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动物源食品安全与HACCP制度 总被引：3，自引：0，他引：3
１何谓HACCPHACCP是英文“Hazardsanalysisandcriticalcontrolpoints”的缩写，意思是“公害（或危害）分析关键控制点”。它是一种用来鉴定、评价和控制食品安全的系统性方法，是在国际上已普遍实施的一种保证食品安全的卫生制度，是被设计用于从农业生产、采办、加工、流通、烹调一直到送上餐桌全过程所有环节的监控，以便有效地预防或消除食品安全公害，或把公害减少到可以接受的水平。HACCP有７个原则，即①公害分析检验，包括会损害食物安全的生物学、化学和物理学的污染状况的检验。②确定关键控制点并在这些控制点上能够实施… 相似文献
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β-内酰胺酶抑制剂与β-内酰胺类抗生素联用在兽医学上的应用 总被引：1，自引：0，他引：1
陈志华 《广东畜牧兽医科技》2003,28(6):17-19
20世纪40年代,第一个β-内酰胺类抗生素青霉素的研制和应用,开创了抗生素治疗细菌性疾病的光辉时代。但细菌的耐药性也因抗生素的广泛应用而变得日益普遍,细菌对β-内酰胺类抗生素最主要的耐药机制是产生β-内酰胺酶,破坏β-内酰胺环使抗生素失活。β-内酰胺酶抑制剂的研制应用,恢复了β-内酰胺类抗生素的有效性,使β-内酰胺类抗生素得以继续发挥其在临床上强大的治疗作用。本文主要综述了β-内酰抑制剂的作用机制及其与β-内酰胺类抗生素联用的抗菌活性,以及在兽医临床上的应用概况。为有效地应用β-内酰胺类抗生素控制耐药菌感染提供参考。 相似文献
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Pathogenicity studies of feline coronavirus isolates 79-1146 and 79-1683 总被引：13，自引：0，他引：13
N C Pedersen J F Evermann A J McKeirnan R L Ott 《American journal of veterinary research》1984,45(12):2580-2585
Two feline coronavirus isolates were characterized by their disease-causing potential in cats. The 79-1683 feline coronavirus isolate caused an inapparent-to-mild enteritis when given oronasally to specific-pathogen-free kittens and was not a cause of feline infectious peritonitis (FIP). Target tissues for the virus were the mature apical epithelium of the small intestine, mesenteric lymph nodes, tonsils, thymus, and (to a lesser extent) the lungs. Inoculated kittens shed high numbers of virus in their feces for 14 to 17 days, but remained infectious to susceptible kittens for longer periods of time, as evidenced by contact-exposure studies. Because the 79-1683 isolate induced only enteritis, it was designated feline enteric coronavirus (FECV) 79-1683. The 79-1146 feline coronavirus isolate induced effusive abdominal FIP in specific-pathogen-free kittens after oronasal and intraperitoneal inoculation. Clinical signs of disease appeared within 12 to 14 days in almost all inoculated kittens. Because this isolate caused FIP, it was designated FIP virus (FIPV) 79-1146. Cross-protective immunity was not induced by the various coronavirus infections. Kittens preimmunized with the UCD strain of FECV (FECV-UCD) or with FECV-79-1683 were not immune to infection with FIPV-79-1146. Likewise, kittens previously inoculated with FECV-79-1683 were not immune to infection with FIPV-UCD1. In fact, preexisting heterologous FECV-79-1683 immunity often accelerated and enhanced the severity of disease caused by inoculation with FIPV-UCD1.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
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