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1.
随着保健养生的意识的增强,人们于利用蜜蜂相关产品的基础上,逐渐地了解且接触并开始应用蜂花粉。蜂花粉中的多酚类及黄酮类化合物提供了良好的抗氧化活性,对人类的健康有很大的益处,可减少人体被自由基攻击。蜂花粉的成分及香气亦会因花粉来源不同而有很大的差异,本研究将针对常见的几种蜂花粉产品进行抗氧化活性测试并比较之间的差异性。在此分别针对油菜花粉、花莲百草花粉、蒲盐花粉、茶花粉、松花粉、含羞草花粉、云林百草花粉等7种不同蜂花粉产品进行抗氧化测试包含清除DPPH自由基能力、清除氢氧自由基能力、清除超氧自由基能力、抑制亚麻油酸能力及还原力等抗氧化活性方法测试,并分析其总蛋白质含量及总酚类含量。结果显示,不同蜂花粉产品因花粉源、制程或加工不同,总酚类含量即不同,进而影响了抗氧化活性之表现。其中以油菜花粉、花莲百草花粉及蒲盐花粉于多项抗氧化能力测定下,具有较佳之抗氧化性效能。 相似文献
2.
海拔对桃儿七果实特性、活性成分含量及抗氧化能力的影响 总被引：1，自引：0，他引：1
为了探明海拔对桃儿七果实特性以及活性成分积累的影响,更好地保护和利用桃儿七野生资源,本研究以不同海拔(2400~2500 m和2900~3000 m)的野生桃儿七果实为材料,分别对果实特性、活性物质含量以及抗氧化能力进行了测定与分析。结果表明,海拔对桃儿七果实特性、活性成分积累以及组织间的差异性具有显著影响。低海拔果实开花期至成熟期的天数为111 d,较高海拔延长5 d,成熟期较高海拔提前13 d,低海拔果实的纵径、横径、干重、种子数量和千粒干重均高于高海拔,而纵横比则相反;低海拔果皮和果肉中可溶性糖、总黄酮和酚类化合物的含量分别显著高于高海拔;低海拔果皮70%和10%乙醇提取液的抑制率、铁离子还原/氧化能力(FRAP值)以及果肉70%乙醇提取液的FRAP值分别显著大于高海拔。同一海拔不同组织而言,果肉中可溶性糖的含量显著高于果皮,总黄酮和酚类含量则相反;在低海拔地区,果皮70%和10%乙醇提取液的抑制率和FRAP值分别显著大于果肉,而在高海拔地区,仅果皮70%乙醇提取液的FRAP值显著大于果肉。因此,较低海拔有利于桃儿七果实的生长与发育以及果实中可溶性糖、总黄酮和酚类化合物等活性物质的积累。 相似文献
3.
兰州百合连作障碍效应及机制研究 总被引：1，自引：0，他引：1
为探讨兰州百合连作障碍效应及作用机制,以正茬及不同连作年限土壤为试验对象,利用盆栽试验,动态研究了连作对百合生长、光合特性及抗氧化酶系统的影响,并利用GC-MS技术分析了正茬及不同连作年限土壤中自毒成分的种类及含量。结果显示,苗期、鳞茎膨大期及成熟期连作对百合的生长均存在抑制作用,且随着连作年限的延长作用程度增强。同一生长阶段,随连作年限的增加,百合植株中CAT和SOD含量逐渐下降,POD活性先升高后降低,MDA含量持续升高。叶绿素总量(Chl)、净光合速率(Pn)、气孔导度(Gs)及蒸腾速率(Tr)呈下降趋势,而胞间CO2浓度(Ci)持续升高。从正茬、连作2年和连作4年土壤中分别鉴定出9种、15种、17种化感自毒物质,主要包括:2,3-丁二醇、间苯二甲酸二辛酯、2,2'-亚甲基双-(4-甲基-6-叔丁基苯酚)和对苯二甲酸二辛酯。其中,对苯二甲酸二辛酯在连作2年和4年土壤中含量均达最高值,分别为33.24%和41.43%。连作通过影响百合植株的光合作用能力,破坏抗氧化酶代谢平衡,从而抑制百合植株的生长,导致连作障碍的发生;连作条件下多种次生代谢物引起的自毒作用是导致兰州百合连作障碍产生的主要原因之一。 相似文献
4.
不同收获期贯叶连翘花中抗氧化能力、主要活性物质变化及挥发性组分分离鉴定 总被引：2，自引：0，他引：2
为了探明不同收获期(花蕾期、盛花期、结果期)贯叶连翘花中抗氧化能力、总黄酮、酚类和金丝桃素含量等的变化,以栽培花器官为实验材料,采用HPLC和GC-MS等方法对70%乙醇提取液进行测定与分析。结果表明,花蕾期和盛花期贯叶连翘花提取液自由基抑制率(inhibition percentage, I%)和铁离子还原/氧化能力(ferric reducing/antioxidant power, FRAP)均显著高于结果期,花蕾期和盛花期二者之间无显著差异;总黄酮和酚类化合物以及金丝桃素含量均呈现为盛花期>花蕾期>结果期,且在P<0.05水平下达到显著差异;盛花期花中分离鉴定得到37种挥发性化学组分,其中,主要成分有1,1-二乙氧基-乙烷(19.26%)、1-十六醇(17.85%)、β-衣兰烯(10.71%)、(Z, Z)-9,12-十八碳二烯酰氯(8.42%)、十六烷酸乙酯(8.40%)、叶绿醇(5.79%)、石竹烯氧化物(4.56%)等。以上研究结果表明,花蕾期至盛花期采集贯叶连翘花器官较佳,提取液抗氧化能力较强,主要活性物质含量较高,挥发性成分较为丰富,该研究结果将对贯叶连翘生产、大面积种植栽培具有重要的参考价值和实践意义。 相似文献
5.
I. Kafantaris B. Kotsampasi V. Christodoulou E. Kokka P. Kouka Z. Terzopoulou K. Gerasopoulos D. Stagos C. Mitsagga I. Giavasis S. Makri K. Petrotos D. Kouretas 《Journal of animal physiology and animal nutrition》2017,101(5):e108-e121
Grape pomace is a by‐product of winemaking process and rich in bioactive compounds such as plant polyphenols having antioxidant and antimicrobial activities. As known, oxidative stress may cause a number of pathological conditions in farm animals and thus affecting animal welfare and production. Moreover, pathogenic bacteria affect animals’ health status. Thus, the objective of this study was to investigate whether lambs’ feed supplemented with grape pomace enhances the antioxidant mechanisms and reduces the growth of pathogenic bacteria. For this purpose, redox status was assessed in blood and tissues using oxidative stress markers in lambs fed with diet supplemented with grape pomace. Moreover, the effect on bacterial growth was assessed by examining microbial populations in lambs’ faecal microflora. The following oxidative stress markers were assessed: reduced glutathione (GSH), catalase activity (CAT), total antioxidant capacity (TAC), thiobarbituric acid‐reactive substances (TBARS) and protein carbonyls (CARB). Twenty‐four lambs were assigned to two experimental groups receiving standard or experimental diet for 55 days. Blood and tissues (liver, quadriceps muscle, spleen, heart and brain) were collected at two different time‐points, 42 and 70 days post‐birth, after ration administration. The results showed that lambs fed with experimental diet had significantly increased antioxidants mechanisms in blood and tissues as shown by increases in CAT and GSH compared to control. Moreover, lambs fed with the experimental diet exhibited decreased oxidative stress‐induced damage to lipids and proteins as shown by decreases in TBARS and CARB respectively. In addition, the experimental diet enhanced the growth of facultative probiotic bacteria and inhibited the growth of pathogen populations such as Enterobacteriacae and E. coli. This is the first study investigating the effect of diet supplemented with grape pomace on the redox status and microbiota of lambs. 相似文献
6.
《畜牧与生物技术杂志(英文版)》2021,(3)
Due to ban on using antibiotics in feed industry, awareness of using natural feed additives have led to a great demand. The interest of plants phenolic compounds as a potential natural antioxidant source has been considered in research community due to their predictable potential role as feed additives in poultry and swine production.However, the mode of action for their functional role and dosage recommendation in animal diets are still remain indistinct. Taking into account, the present review study highlights an outline about the mode of action of phenolic compound and their experimental uses in poultry and swine focusing on the growth performance, antioxidant function, immune function, antimicrobial role and overall health status, justified with the past findings till to date.Finally, the present review study concluded that supplementation of phenolic compounds as natural feed additives may have a role on the antioxidant, immunity, antimicrobial and overall production performance in poultry and swine. 相似文献
7.
为分析不同李种质果皮总酚含量及其抗氧化能力,以21个李种质为试材,测定果皮中总酚含量,采用DPPH法、FRAP法和ABTs法测定其抗氧化能力。结果表明：李果皮中酚含量丰富且不同种质间差异明显,不同李种质果皮中总酚含量为66.39~560.81mg/100g,平均含量224.23mg/100g,变异系数为48.24%,安哥诺、黑宝石、密山大紫李、青奈中含量较高,玉皇李含量最低;李果皮酚提取物具有较强的抗氧化能力,综合抗氧化能力较强的品种有安哥诺、黑宝石、密山大紫李、青奈和布尔班克。果皮中总酚含量与DPPH、FRAP和ABTs 均呈极显著正相关。3种抗氧化评价方法的相关性分析表明,DPPH/FRAP、DPPH/ABTs和FRAP/ABTs相关性均达到极显著相关,3种方法可作为李果皮酚类物质抗氧化能力评价的主要选择。 相似文献
8.
Bizimenyera SE Swan GE Chikoto H Eloff JN 《Journal of the South African Veterinary Association》2005,76(2):54-58
Peltophorum africanum (Fabaceae) is a deciduous tree widespread in southern Africa. The plant has many ethnomedical and ethnoveterinary uses. Root and bark decoctions are used to treat diarrhoea, dysentery, sore throat, wounds, back and joint pains, HIV-AIDS, venereal diseases and infertility. Pastoralists and rural farmers use the root and bark extracts to treat diarrhoea, dysentery, infertility, and to promote well-being and resistance to diseases in cattle. To evaluate these ethnobotanical leads, dried leaves, stem bark and root bark were extracted with ethanol, acetone, dichloromethane and hexane. Polyphenols in the extract were determined by the Folin-Ciocalteu method with gallic acid as standard. Qualitative antioxidant activity was screened by spraying thin layer chromatograms (TLC) of the extracts with 0.2% 1,1-diphenyl-2-picryl hydrazyl (DPPH), and quantified with Trolox equivalent antioxidant capacity (TEAC) assay. Minimum inhibitory concentration (MIC) and total antibacterial activity (TAA) were determined by serial microplate dilution for Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Enterococcus faecalis, with gentamicin as standard and tetrazolium violet as growth indicator. Acetone and ethanol extracted the largest quantity of material. Polyphenols concentration was 49.2% in acetone extract of the root and 3.8% in dichloromethane extract of the leaf. Antioxidant activity of at least 5 antioxidant compounds as measured by TEAC ranged from 1.34 (ethanol extract of the root) to 0.01 (hexane extract of the leaf). The total antibacterial activity (volume to which active compounds present in 1 g plant material can be diluted and still inhibit bacterial growth) was 1263 ml/g for ethanol extract of the root against S. aureus, and 800 ml/g for acetone extract of the root against P. aeruginosa. There was substantial activity against both Gram-positive and Gram-negative bacteria, with MIC values of 0.08 mg/ml for S. aureus and 0.16 mg/ml for P. aeruginosa. There is therefore a rationale for the traditional use of root and bark of P. africanum in treating bacterial infection related diseases. 相似文献
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10.
试验旨在研究妊娠母羊饲喂竹酢粉对羔羊生长性能、血液生理生化指标、免疫与抗氧化能力的影响。选择初产、妊娠约120 d的健康湖羊50只，随机分为2组，分别饲喂基础日粮（对照组）和基础日粮+2%竹酢粉（试验组），饲喂3周，预饲期1周。分娩后，选择单羔母羊所产、3周龄左右、产期相近（1~4 d）羔羊20只（对照组和试验组各10只）测定其生长性能、血液生理生化、免疫与抗氧化能力等指标。结果表明：试验组羔羊的初生重（BW）、平均日增重（ADG）与对照组相比差异不显著（P>0.05），但日增重提高4.74%。与对照组相比，试验组羔羊血液中白细胞、红细胞、血小板数量和血红蛋白浓度均无显著差异（P>0.05）；血清谷草转氨酶（GOT）活性显著降低（P<0.05），总蛋白（TP）、白蛋白（ALB）、球蛋白（GLB）含量和白蛋白/球蛋白（A/G）有一定程度提高，总胆固醇（TC）含量、丙二醛（MDA）含量、超氧化物歧化酶（SOD）活性和总抗氧化能力（T-AOC）均有降低趋势（P>0.05）；试验组羔羊免疫球蛋白IgA、IgM和IgG含量高于对照组，但均无显著差异（P>0.05）。综上所述，在妊娠母羊日粮中添加2%竹酢粉可在一定程度上提高羔羊日增重，同时羔羊血清总蛋白、白蛋白、球蛋白、IgA、IgM和IgG含量也有一定程度提高。 相似文献
11.
对夏枯草（Prunella vulgaris）不同溶剂提取物体外抗氧化活性进行评价,为进一步开发利用夏枯草提供参考。采用70%乙醇超声波提取,然后用等体积的乙酸乙酯、正丁醇和水萃取,得到乙酸乙酯、正丁醇和水提取物,并以BHT为对照,研究不同溶剂提取物的抗氧化活性。结果表明,夏枯草提取物具有良好的抗氧化性。不同溶剂提取物及BHT清除DPPH·的能力高低次序为乙酸乙酯提取物＞正丁醇提取物＞BHT＞水提物,清除·OH的能力高低次序为BHT＞乙酸乙酯提取物＞正丁醇提取物＞水提物,清除O-2·的能力高低次序为乙酸乙酯提取物＞水提物＞BHT＞正丁醇提取物,还原力高低次序为BHT＞乙酸乙酯提取物＞正丁醇提取物＞水提物。夏枯草乙酸乙酯提取物具有较强的抗氧化活性。 相似文献
12.
前期试验筛选出了生长迅速、性状优良的Epichlo? sinensis内生真菌菌株,为进一步利用这些性状优良的菌株,需筛选出适宜E.sinensis生长的最佳碳氮源,本研究分别采用不同碳源(葡萄糖、淀粉、麦芽糖、甘露醇、山梨醇)和氮源(氯化铵、蛋白胨、胰蛋白胨、酵母浸粉、尿素)的固体培养基及液体培养基,对3株E.sinensis内生真菌菌株(菌株ID:1、41C和111C)进行培养,探究不同碳氮源条件下,E.sinensis的生长状况及总抗氧化能力.结果表明:供试的3株E.sinensis菌株的生长有显著差异(P<0.05),在所有的碳源和氮源培养基上,菌株111C的菌落直径、生长速率、菌丝鲜重及总抗氧化能力均显著高于菌株1和41C(P<0.05),菌丝直径低于菌株1和41C.而菌株41C的菌落直径及菌丝鲜重显著高于菌株1(P<0.05),总抗氧化能力及菌丝直径显著低于菌株1(P<0.05).不同菌株适宜的碳源和氮源亦不相同,在供试的5种碳源和氮源中,菌株111C的最佳碳氮源为麦芽糖和胰蛋白胨;菌株41C的最佳碳氮源为葡萄糖和胰蛋白胨;菌株1的最佳碳氮源为葡萄糖和胰蛋白胨;且在以酵母浸粉为氮源的条件下,3株内生真菌的总抗氧化能力最强.本研究结果表明,菌株111C生长迅速,总抗氧化能力强,可用于后续研究利用且初步研究获得适宜E.sinensis生长的最佳碳氮源. 相似文献
13.
为探讨青藏高原高山嵩草（Kobresia pygmaea）叶、根抗寒性生理特征,本研究对返青期、草盛中期、草盛后期和枯黄期青藏高原高山嵩草叶和根中丙二醛、脯氨酸、可溶性糖含量及超氧化物歧化酶、过氧化物酶和过氧化氢酶活性进行测定。结果显示：叶片中丙二醛和可溶性糖含量在枯黄期达到最高,而脯氨酸含量在返青期最高;根中丙二醛含量在植物生长后期较低,可溶性糖和脯氨酸含量在后期则较高;叶片中3种抗氧化酶活性在植物生长后期呈显著下降趋势（P<0.05）;而根中3种抗氧化酶活性在植物生长后期较强;不同物候期各生理指标叶片中含量均高于根;器官、物候期以及器官和物候期的交互作用能够显著影响高山嵩草的渗透调节物质和抗氧化酶活性（P<0.05）,且不同物候期其不同器官生理指标的相关性不同。综上所述,高山嵩草叶片和根中的渗透调节物质和抗氧化系统对不同物候期的环境变化积极响应,这可能是其适应高寒环境的生理机制。 相似文献
14.
Fébel H Mézes M Pálfy T Hermán A Gundel J Lugasi A Balogh K Kocsis I Blázovics A 《Journal of animal physiology and animal nutrition》2008,92(3):369-376
The effects of dietary fat supplementation on performance, fatty acid (FA) composition of tissues and antioxidant defence system of broilers were studied. Male broilers were placed in 20 floor pens (60 broilers per pen). The broilers were fed by diets with added different energy sources: lard (L); sunflower oil (SFO); soybean oil (SBO); and linseed oil (LSO). The treatments did not modify significantly growth performance and feed intake of the broilers. There was no effect of dietary FA pattern on reduced glutathione level and glutathione peroxidase activity of plasma, erythrocyte and liver samples. However, higher PUFA content of the diet resulted in a significant increase in malondialdehyde level of erythrocytes and liver. The broilers fed LSO diet more effectively maintained their antioxidant status with enhanced plasma radical scavenger capacity. FA composition in tissues reflected the FA pattern of the diets, although proportion of FAs with four or more double bonds was metabolic specific. LSO diet increased the level of C18:3, C20:5 and C22:6 in tissue lipids in relation to L, SFO and SBO diets. Significantly increased plasma radical scavenging capacity in concert with the enhanced C20:5 and C22:6 proportion in liver and muscle during LSO feeding indicate metabolic changes to counteract the oxidative injury. This may be related to the compounds produced after different biochemical pathways of n-6 and n-3 FAs. 相似文献
15.
不同产地蜂胶的抗氧化活性 总被引：1，自引：0，他引：1
不同地区生产的蜂胶,由于其化学成分不同,因而抗氧化活性也不同。比较了产自阿根廷、澳大利亚、巴西、保加利亚、智利、匈牙利、新西兰、南非、泰国、乌克兰、乌拉圭、美国、乌兹别克斯坦以及中国(河北、湖北和浙江)的蜂胶乙醇提取液的抗氧化活性以及总酚酸类、黄酮类的含量,结果表明:产自阿根廷、澳大利亚、中国、匈牙利以及新西兰的蜂胶醇提取液具有很强的抗氧化活性,并且抗氧化活性与其总酚酸类及黄酮类含量相关,其中主要的抗氧化组分为堪菲醇和咖啡酸苯乙酯。 相似文献
16.
Simone Mancini Sara Minieri Arianna Buccioni Margherita Marzoni Fecia di Cossato Claudia Russo Gisella Paci 《Animal Science Journal》2019,90(5):680-689
Tannins were recently evaluated as feed additives in order to increase antioxidant compounds in animal diet, mainly to enhance resistance to lipid oxidation in meat. Rabbit meat is one of the most susceptible animal products, thus the main aim of this study was to evaluate the capacity of tannins to elongate shelf life of rabbit meat. Ninety hybrid rabbits were fed with three different diets: basal diet (control, C) and basal diet supplemented with 0.3% or 0.6% of tannins mix. Meat samples were refrigerated as raw at 4°C up to 11 days and analysed both as raw and cooked for physical‐chemical characteristics, fatty acids profile, lipid oxidation and antioxidant capacity. Results showed that dietary tannins affected meat colour of raw samples (mostly yellowness). Lipid peroxidation (TBARS) of raw samples was lower in tannins group than C group; a further inhibition of peroxidation was showed also in cooked samples only by the highest dose of tannins mix. Moreover, antioxidant capacity (ABTS) of raw samples increased with the percentage of tannins. In conclusion, supplementation with 0.6% of tannins mix seems to positively affect the lipid peroxidation and antioxidant capacity of meat without modifying the intrinsic characteristics of rabbit meat. 相似文献
17.
Exercise has been shown to increase the production of reactive oxygen species (ROS) to a point that can exceed antioxidant defenses, to cause oxidative stress. The aim of our trials was to evaluate oxidative stress and recovery times in trained dogs during two different hunting exercises, with reactive oxygen metabolites-derivatives (d-ROMs) and biological antioxidant potential (BAP) tests. A group of nine privately owned Italian hounds were included. A 20-min aerobic exercise and a 4-h aerobic exercise, after 30 days of rest, were performed by the dogs. Our results show an oxidative stress after exercise due to both the high concentration of oxidants (d-ROMs) and the low level of antioxidant power (BAP). Besides, the recovery time is faster after the 4-h aerobic exercise than the 20-min aerobic exercise. Oxidative stress monitoring during dogs exercise could become an interesting aid to establish ideal adaptation to training. 相似文献
18.
为探究琥珀酸黄杆菌(FS)对遮阴胁迫下多年生黑麦草(LP)的缓解效应,在盆栽试验条件下,研究了不同遮阴胁迫下(0、20%、40%、60%和80%)接种琥珀酸黄杆菌DSM4002(FS)对LP生长和生理特性的影响。结果表明,遮阴胁迫显著抑制LP的生长,而FS能够有效缓解遮阴胁迫对LP的生长抑制。60%和80%遮阴导致多年生黑麦草积累较高的活性氧和丙二醛,FS处理有效地缓解了遮阴对LP幼苗造成的氧化损伤,20%遮阴+FS、40%遮阴+FS、60%遮阴+FS和80%遮阴+FS处理中氧自由基含量分别与单独遮阴处理相比下降4.47%、29.75%、44.57%和49.84%。遮阴胁迫不同程度地改变了超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)、谷胱甘肽还原酶(GR)活性以及抗坏血酸(AsA)和还原性谷胱甘肽(GSH)含量,接种FS能够进一步提高SOD、CAT、GR活性及AsA和GSH水平,但是接种FS仅能够显著提高40%遮阴胁迫下POD活性和60%遮阴胁迫下APX活性。由此可见,遮阴胁迫下接种FS能够显著提高LP的抗氧化系统活性,增加活性氧的清除能力,减轻遮阴引起的氧化损伤,从而增强LP的耐阴性。 相似文献
19.
水土保持植物类芦对土壤酸胁迫的形态生理响应 总被引：3，自引：0，他引：3
土壤酸度低是限制一些金属矿山废弃地植被恢复的主要因素之一,类芦可在酸度极低的稀土矿废弃地正常生长,其中可能存在对酸胁迫特殊的形态生理响应机制。因此,采用室内土培盆栽模拟胁迫试验,设计不同强度酸胁迫处理(pH: 3.5,4.5,5.0和5.6),测定不同酸胁迫条件下,类芦地上部分和根系生长、生物量、抗氧化酶活性及MDA含量等指标,探讨类芦对土壤酸胁迫的适应策略。结果表明,类芦对土壤酸胁迫有一定的耐性,pH 3.5处理仍能较正常生长;随土壤pH的逐渐增大,类芦地上部分和根系各生长指标整体表现为先增大后减小的变化趋势,弱酸胁迫对类芦根系生长有一定促进作用,pH 4.5处理均达最大值,为类芦最适宜生长的土壤酸度;酸胁迫条件下,类芦可通过加快根直径的生长,增大根系的生物量分配,以适应酸胁迫条件,但酸胁迫对类芦体内抗氧化酶活性具有一定抑制作用。 相似文献
20.
研究紫花苜蓿（Medicago sativa L.）叶总黄酮的最优提取工艺及体外抗氧化能力。在单因素试验的基础上设计正交试验,通过方差分析和多重比较确定最优提取工艺。以1,1-二苯基-2-三硝基苯肼（2,2-diphenyl-1-picrylhydrazyl,DPPH）清除率测定法及还原力测定法评价苜蓿黄酮类化合物体外抗氧化活性。最佳提取工艺条件是：乙醇体积分数70%、提取温度60℃、提取时间30 min、超声功率180 w,总黄酮得率为6.43 mg·g-1。苜蓿叶总黄酮在一定的质量浓度范围具有较明显的抗氧化活性,并随着质量浓度的增加活性增强。苜蓿叶总黄酮具有较强的还原力,清除DPPH自由基的半数抑制质量浓度（IC50值）为0.608 mg·mL-1。超声提取是一种高效的提取紫花苜蓿叶总黄酮方法。超声优化的苜蓿叶总黄酮提取工艺经济、稳定、合理可行。 相似文献