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1.
Summary. Translocation of 3-amino-1,2,4-triazole-5-14C (14C-aminotriazole) was compared to that of 14C-assimilates in couch grass ( Agropyron repens (L.) Beauv.) at three different growth stages.
Assimilates of 14CO2 were translocated from the treated shoot to other shoots and rhizomes at the 2–3-leaf and 3–4-leaf stages of development. Much less labelled material was translocated into untreated shoots at the 5-leaf stage. More 14C-assimilates were translocated to the roots than to untreated shoots at all developmental stages. The translocation patterns of 14C-aminotriazole and 14C-assimilates were similar.
Two metabolites, A and B, were formed from 14C-aminotriazole, which chromatographed identically to previously described metabolites in Cirsium arvense (L.) Scop. A was further metabolized into B. Labelled aminotriazole and its two metabolites were translocated throughout the plants. Metabolite A was phytotoxic when concentrated and re-applied to couch grass, but its properties were not those of Unknown II from Cirsium arvense. They were the same as those of Unknown III. Whether or not metabolite A and Unknown III are identical was not established.
Migration des substances assimilables marquées au 14 C, du 3 amino-1,2,4-triazole et de ses métabolites chez Agropyron repens  相似文献   

2.
Summary Plants of Paspalum distichum L. with two stolons growing over water were treated separately with the sodium salt of 2,2-dichloropropionic acid-36C1 (dalapon), 3-amino-1,2,4-triazole-14C (amitrole) and 1,1'-dimethyl-4,4'-dipyridylium dichloride-14C (paraquat) at four different situations on one stolon. Autoradiographs showed that both the amitrole and dalapon label accumulated in the apices of the treated stolons and in some of the young shoots growing from the base of the plant. The 14C from the paraquat label showed predominantly xylem translocating with no apical accumulation and little basal translocation. The results indicate that young basal shoots rely to some extent on assimilates from the mature stolons but that there is little transport between mature stolons.
La migration et la distribution de trois Herbicides marqués dans Paspalum distichum L.  相似文献   

3.
Summary. Previous findings suggested that the translocation in Agropyron repens of 2,2-dichloropropionic acid (dalapon) may be considerably affected by the transpiration rate. This relationship has been studied further in the present investigation using 14C-labelled material and autoradiography.
It was found that when dalapon was applied to the leaves and the treated plants were placed in darkness a reduction in the transpiration rate of ca. 90% was associated with a marked increase in the amount of dalapon translocation into the roots and tillers. The evidence provided by the autoradiographs was confirmed by a quantitative assay of the 14C present in the roots. It was also found, however, that translocation was not appreciably affected either by a 50% reduction in the light intensity or when the transpiration rate was reduced by placing the plaints under conditions of high humidity in the light or by the application of white petroleum jelly to the leaves. An alternative hypothesis, namely that the effect of darkness on the movement of the herbicide might be due to changes induced in the normal pattern of assimilate translocation was also investigated but was not supported by the results obtained. Further experimentss, in which various parts of the treated shoot were placed in the dark, suggested that the effect of darkness on the translocation of dalapon is exerted primarily on the treated leaf itself.
Etudes sur to migration dans Agropyron repens de l'acide 2,2-dichloropropionique marqué avec 14C  相似文献   

4.
Summary. High concentrations of 2,4-D and picloram interfered with the downward movement of 14c-assimilates infield-grown vines. The interference in translocation was appreciably greater with picloram than it was with 2,4-D, Although basipetal translocation was retarded, translocation within the treated shoots continued from the vegetative part to the clusters. Translocation of 2,4-D appeared to follow the same route as 14c-assimilatcs for the most part. Formative effects were absent on untreated grape shoots although the adjacent shoots treated with 2,4-D or picloram on the same cordons were killed; however, formative effects were evident on some of the stump sprouts which developed after the vines were harvested. The malformed leaves on the stump sprouts were twelve or more nodes from the base of the shoots, while 14c was in the more basal leaves.
Thompson Seedless (Sultanina) rootings treated with 20 000 ppm 2,4-D or picloram transported less 14C to the roots than did the controls. Treatment with either herbicide resulted in a marked increase in the labelling of the stems.
Effet du 2,4-D et du pichlorame sur la migration de métabolites marqués au 14C dans Vitis vinifera L.  相似文献   

5.
Summary. Plants of Potamogeton nodosus, a submersed aquatic, were treated with the di-sodium salt of 7-oxabicyclo(2,2,l)heptane-2,3-dicarboxylic acid-14C (endothal-14C). Gross radioautographs showed that the 14C label moved from mature photosynthesizing leaves and accumulated in the apices and developing secondary plants. Similar results were obtained with mid-leaf, stem (internode) and winter-bud applications. No move-ment of radioactivity occurred following root treatment. The results indicate symplastic translocation of endothal-14C when applied to the leaf, stem or winter bud. It is suggested that endothal can cause the death of plants by direct injury to root tissues subsequent to absorption.
Recherches sur la migration de l'endothal-14C dans le Potamogeton nodosus Poir.  相似文献   

6.
Summary. 3,4,3',4'-Tetrachloroazobenzene (TCAB) and TCAB-14C were supplied to the roots of rice ( Oryza sativa L.) plants in nutrient solution. An analysis of the shoots indicated that the TCAB was absorbed and translocated to the shoots. The translocated material was characterized by gas-liquid chromatography and mass spectrometry. Roots were exposed to saturation concentrations of TCAB-14C in liquid culture to determine the translocation and distribution of the compound. Only 5'6% of the total azobenzene present in the nutrient solution was absorbed by the intact rice plants and only 3'2% of the absorbed TCAB-14C was translocated to the shoots after 12 days of treatment. No TGAB was isolated from plants treated with propanil or 3,4-dichloroaniline after 14 days of treatment.  相似文献   

7.
Summary. A method of exposing seedlings of Norway spruce (Picea abies (L.) Karst.) to 14CO2 is described. Within the 1st hr alter 14CO2 exposure, no translocation of the 14C out of the treated branch could be observed. After a 24-hr period, however, the 14C in dormant seedlings had been translocated basipetally to part of the root system only, with no lateral diffusion of the 14C-compounds in the stem. About a week after exposure, both symplastic and apoplastic patterns of translocation had caused a more uniform distribution of 14C. In seedlings at active internode elongation, the translocation patterns were fundamentally identical to those in dormant seedlings, but the active shoot growth had led to a more uniform distribution of the 14C.
Simazine at 20 ppm had apparently stimulated both the photofixation of 14CO2 and the rate of translocation of the 14C-assimilates. At 30 ppm, however, simazine had blocked the translocation of nutrients to the roots. On the other hand, the 14CO2 uptake was not influenced. The simazine incubation had apparently no influence on the synthesis of cationic photosynthate.  相似文献   

8.
Growth chamber experiments were conducted in order to study the absorption, translocation and activity of glyphosate when applied to roots with aqueous solution avoiding any glyphosate–substrate interaction. Corn seedlings at the first leaf stage were set up in individual graduated cylinders containing different solutions of 14C-glyphosate (0–30 mg ae kg−1). After 26 h of root exposure, plants were transferred to fresh nutrient solution and grown for the next 5 days. After harvest, plants were separated into seed, root, mesocotyle, coleoptile, cotyledon, first leaf and all new leaves (apex), and quantified 14C radioactivity contained in each part. Glyphosate uptake was only 11% of the theoretical mass flow into the plant. The amount of glyphosate translocated from roots was positively correlated with plant uptake ( P  < 0.01). Total plant fresh weight presented a logistic response to glyphosate amounts, including a growth stimulant effect (hormesis), when plants absorbed less than 0.6 µg. The treated plants presented a normal pattern of glyphosate allocation, with the apex the principal sink, accumulating more than 38% of mobilized glyphosate. When corn plants absorbed more than 0.6 µg they showed a decrease in growth. The relatively high glyphosate quantities allocated in the new leaves showed the relevance of the symplastic pathway in the translocation process for root absorbed glyphosate.  相似文献   

9.
Rooted cuttings of Pyrus melanocarpa (Michx.) Willd. had a 3.5-fold greater tolerance to soil-applied hexazinone than those of Rubus hispidus L. in a greenhouse trial. R. hispidus accumulated four times more 14C-label in the foliage following root uptake of 14C-hexazinone than P. melano-carpa . Here, the greater uptake and susceptibility of R. hispidus was related to its greater root:fol-iage (weight) ratio compared to the tolerant P. melanocarpa . However, in whole plant metabolism studies employing younger plants there were no differences in 14C accumulation in the leaves. Here 14Chexazinone was converted to a number of hydroxylated and/or demethylated triazinone metabolites in both species following vacuum infiltration into leaf disks and root sections, or following root uptake in whole plants. A major difference in metabolism between the two species was the greater formation of the mono demethylated metabolite, B, [3-cyclohexyl-6-methylamino-l-methyl-l,3,5-triazine-2,4-dione] in P. melanocarpa which may also contribute to its greater tolerance. A loss of total 14Clabel from all plant parts with time may reflect cleavage of the 14C-ring-labelled herbicide.  相似文献   

10.
[14C]-Imazethapyr was applied as a seed treatment and at plant pre-emergence and post-emergence to peas ( Pisum sativum L.) parasitized by Orobanche crenata Forsk. Herbicide uptake increased with time regardless of the application method. Uptake reached about 98%, 89%, 81% and 94% of the total herbicide applied for the seed coating, seed soaking, pre-emergence and post-emergence treatments respectively. Herbicide translocation within the host plants consistently differed between O. crenata -infected and non-infected plants. High levels of 14C activity were accumulated by parasitic plants from the host. In non-infected pea plants, pods were stronger sinks for imazethapyr than the other parts of the plant, regardless of the application method. The herbicide distribution in the pea plant: O. crenata complex showed the same pattern regardless of the application methods. However, accumulation of radioactivity in the parasite was lower with pre-emergence and post-emergence application than with the seed treatments. In addition, radioactivity concentration in O. crenata plants was slightly higher when [14C]-imazethapyr was applied to pea seeds by coating than by soaking.  相似文献   

11.
Summary. 14CO2 together with autoradiographic techniques was used to stuy the movement of labelled carbon within wheat plants at different stages in their development. Movement of 14C about the plain was found to be extensive in the early stages, gradually bccoming more localized until at a stage after ear emergence there was little transference of labelled carbon between tillers within a 24-hour period.
In the early stages of development 14C from leaves on the main shoot was transported throughout the plant but accumulated in greatest amounts in the meristematic regions. Assimilates produced by the leaves of newly formed tillers were distributed mainly to the meristematic regions of those tillers although labelled carbon was moved in into the rest of the plant. In the later stages of development of each tiller the labelled assimilate was restricted to the tiller itself and to any very young tillers associated with it. After car emergence the pattern of distribution of the labelled carbon from the youngest and second youngest leaves was found to differ. Movement was predominantly towards the ear from the flag leaf and towards the root system from the leaf below the flag.
It was noticed that mature leaves accumulated some labelled material and the possibility that this occurred via the transpiration stream following migration from phloem to xylem, as is the case with some of the case with some of the translocated herbicides, was investigated, but no indication of xylem transference was obtained.
The possible application of these studies to investigations into the relationship between the movement natural assimilates and of translocated herbicides is discussed.  相似文献   

12.
Summary. The effects of localized herbicide placement at different internodes of pea ( Pisum sativum L. cv. Alaska) shoots below the soil surface after plant emergence were studied by removing the soil from around the shoots and replacing with herbicide-treated soil. The first internode proved insensitive to linuron, diuron, atrazine and simazine at 4·5 kg/ha, while treatment of the second and third internodes markedly reduced plant growth 4–6 days after treatment. Separate exposure of the first internode alone, and the second and part of the third together to 14C-labelled atrazine indicated no difference in herbicide metabolism. However, a two- to threefold increase in 14C uptake and movement to the foliar parts occurred when the second and part of the third internode was treated, as compared to first internode treatment. Thus the differential sensitivity of the internodes to atrazine, and possibly to the other herbicides, may be because the more mature first internode allows less uptake and subsequent movement to the foliar parts.
Absorption dans la zone des tiges des herbicides appliqués dans le sol chez Pisum sativum L.  相似文献   

13.
Summary. An autoradiographic study of the distribution of 14C-labelled assimilates, derived from 14CO2 fed to the aerial parts, was made at ten different developmental stages of Oxalis latifolia H.B.K. and Oxalis pes-caprae L. Changing patterns of distribution of labelled assimilates to the underground parts of both species were revealed. These patterns are discussed in the context of the timing of control by defoliation and by foliageapplied herbicides.
Etude autoradiographique de la distribution des métabolites marqués an 14C à différents stades de développement de l'Oxalis latifolia H.B.K. et de l' O. pes-caprae L.  相似文献   

14.
Absorption and translocation of 14C-glyphosate was evaluated under controlled conditions in peas ( Pisum sativum L.) and broad beans ( Vicia faba L.) parasitised or not by crenate broomrape Orobanche crenata Forsk.). Absorption increased with time up to 12 days after treatment, and reached about 50% of the 14C-glyphosate applied. Three days after treatment 70–85% of the total herbicide absorbed had been translocated out of the treated leaflet. There was no consistent differ ence in absorption and translocation between infected and non-infected plants 12 days after treatment. The 14C-glyphosate concentration in the root system increased with time in broad beans. In peas it remained more or less constant from 3 days after treatment. Generally, pods were stronger sinks for glyphosate than other parts of the plant. Herbicide accumulation in broomrape increased with its growth stage, and the parasite was a much stronger sink for glyphosate than the legume root system.  相似文献   

15.
Summary. The main shoot or selected tillers of seedling plants of Poa pratensis L. were supplied with 14CO2 and the distribution of 14C-assimilates was followed by autoradiography. The primary tillers were initially supplied with 14C-assimilates from the main shoot but, once established, became independent of the parental shoot for carbohydrates. Similarly, developing rhizome-tillers were supported by the main shoot and established primary tillers but soon became self-supporting, Defoliation of all the tillers of the plant but one led to the defoliated primary and rhizome tillers being supplied with radiocarbon from the remaining intact shoot. Thus, although the primary tillers and rhizome-tillers in the intact plant appear to be physiologically independent, the entire tiller-rhizome system may be re-integrated after defoliation, allowing assimilate redistribution. P. pratensis may be regarded as a convenient link between the non-weedy herbage grasses which generally lack rhizomes and the weedy rhizomatous grasses typified by Agropyron repens (L.) Beauv.
Etat des relations entre les talles et les rhizomes de Poa pratensis L. Etude autoradiographique  相似文献   

16.
Absorption, translocation and distribution of 14C-glyphosate were examined in Agropyron repens (L.) Beauv. plants growing under field conditions in the autumn. Glyphosate absorption did not increase beyond 3 days after application, whereas translocation to the rhizomes continued up to 7 days after application. The translocated glyphosate accumulated more in new rhizomes than in older parts of the rhizomes. Ten per cent of the glyphosate translocated out of the treated shoot was recovered in younger shoots 7 days after application. Plants harvested the following spring contained less than 20% of the glyphosate originally applied. Although a growth cabinet experiment indicated that 34% of the glyphosate in the rhizomes of treated plants could be remobi-lized into new aerial shoots, considerably less was recovered in new, aerial shoots in the spring in the field-grown plants. Freezing experiments showed that glyphosate translocation to the rhizomes was only prevented when cold treatment caused visible damage to A. repens foliage.  相似文献   

17.
Summary. Studies on the physiological effects of dalapon in Cynodon dactylon (L.) Pers. were carried out. Field and glasshouse experiments showed that dalapon is freely translocated in the aerial organs and from these to the rhizomes. No transformation of the herbicide was detected during 72 h following application. In rhizomes as well as in stolons, dalapon caused growth inhibition and necrosis of buds while root growth was apparently not affected. This differential susceptibility was confirmed by culturing node explants in nutrient solution containing dalapon. Although dalapon killed aerial organs, most rhizomes were capable of recovery even when a high dose was applied. These results are discussed. Effets physiologiqius du dalapon sur Cynodon dactylon (L.) Pers.  相似文献   

18.
Summary. When columns of three soil types were perfused with a microbial medium containing 5·7 and 26·9 ppm 14COOH-labelled chloramben, there was a rapid decrease in the 14C activity remaining in solution, attributable to soil adsorption. Soil pH and organic matter content were the primary factors controlling chloramben adsorption. Soil pH may enhance adsorption through its effect on the number of soil anion exchange sites, and sites for polyvalent cation bridging and H bonding. Following adsorption, liver decreases in solution 14C-chloramben activity were attributed to degradation; 14CO2 evolved during perfusion amounted to 25–30% of the total decrease in 14C-chloramben from solution. It was concluded that more than 90% of the 14C-activity remaining in solution could be attributed to intact chloramben. Thus, decarboxylation appeared to be the primary mechanism of chloramben degradation in soils. Lag phases observed during degradation and subsequent investigations with re-amended solutions implicated the soil microflora; even at chloramben concentrations of 26.9 ppm no toxic effect on the chloramben-degrading micro-organisms was apparent.
Dégradation du chlorambène [amibène) dans le sol  相似文献   

19.
Summary. When seeds of lettuce ( Lactuca sativa L.) were placed on small squares of filter paper moistened with solutions of picloram (4-amino-3,5,6-trichloropicolinic acid), the degree of inhibition on of root and hypocotyl elongation 72 hr later was related to picloram concentration. Inhibition was a useful parameter in determining quantities of picloram ranging from 0·03 to 7·2 μg. (Growth stimulation occurred from 0·00072 to 0·0072 μg. After paper chromatography of plant exudates containing 14C-labelled picloram, location and quantities of picloram on the chromatograms were determined by the lettuce bioassay and compared with determinations by the methods of ultraviolet light sensitivity, 14C 4π strip scanning and 14 C dilution calculations. Rf values determined lay all methods were identical, and the quantitative determination by the bioassay agreed closely with the calculations based on the amount of 14C detected by liquid scintillation counting.
Un essai biologique rapide d'idntification et de dosage de piclorame en solution aqueuse  相似文献   

20.
The patterns of absorption and translocation of 14C clopyralid commercially formulated as the acid, monoethanolamine salt, potassium salt, 2-ethylhexyl ester and 1 -decyl ester were compared in Cirsium arvense and Polygonum convolvulus grown under three environmental regimes. Plants were grown under a 35% or 65% RH regime in silica sand maintained at 33% w/w moisture or under a water stress regime at 65% RH. Approxi mately 26,39,86,93 and 100% of the applied 14C-activity from the 2-ethylhexyl ester, acid, monoethanolamine salt, 1-decyl ester and potas sium salt, respectively, were recovered 72 h after application to glass cover slips placed in the growth room. However, loss of applied 14C-activity after application of the five formulations to both plant species was significant only for the 2-ethylhexyl ester. Regardless of environmental regime, the acid was the most readily absorbed formulation. In contrast to the acid, the salts and esters were less readily absorbed. When the data were expressed as a percentage of absorbed radioactivity, there was no significant difference in translocation when the acid, monoethanolamine salt and potassium salt were compared. However, significantly smaller quantities of the absorbed 2-ethylhexyl and 1-decyl ester were exported from the treated leaf. These results indicate that once absorbed, the esters do not readily partition out of the cuticle, whereas the acid and two salts move into the symplast for subsequent translocation. When the results of the three environmental regimes were compared, the absorption of the monoethanolamine and potas sium salts were greatly reduced under low humi dity or water stress, whereas the acid and esters were not affected.  相似文献   

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