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鸡传染性法氏囊病病毒野毒株VP2基因高可变区酶切位点分析 总被引：5，自引：1，他引：4
参照澳大利亚鸡传染性法氏囊病病毒（ Ｉ Ｂ Ｄ Ｖ）００２７３ 毒株基因组序列设计的 １ 对引物,位于 Ｖ Ｐ２ 高可变区两端,通过 Ｒ Ｔ Ｐ Ｃ Ｒ,扩增了 ５ 个 Ｉ Ｂ Ｄ Ｖ 山东分离株 Ｖ Ｐ２ 基因的 ６０７～１ ０８０ 位核苷酸片段（ａａ２０３～３０６）。 Ｐ Ｃ Ｒ 产物经纯化后,分别用 Ｄｒａ Ⅰ、 Ｓａｃ Ⅰ、 Ｓｓｐ Ⅰ、 Ｐｓｔ Ⅰ和 Ｓａｕ３ Ａ Ｉ共 ５ 种限制性内切酶（ Ｒ Ｅ）进行消化处理,结果 ５ 个分离株均为 Ｄｒａ Ⅰ（－ ）、 Ｓａｃ Ⅰ（－ ）和 Ｓａｕ ３ Ａ Ｉ（＋ ）, Ｌ Ｃ２ 和 Ｔ Ａ 株为 Ｓｓｐ Ⅰ（＋ ）, Ｌ Ｃ１ 和 Ｊ Ｎ 株为 Ｓｓｐ Ⅰ（－ ）, Ｌ Ｃ１ 和 Ｌ Ｃ２ 株为 Ｐｓｔ Ⅰ（＋ ）, Ｊ Ｎ、 Ｌ Ｌ 和 Ｔ Ａ 株为 Ｐｓｔ Ⅰ（－ ）;５ 个分离株的酶切图谱与美国变异株迥异,而 Ｔ Ａ 株与日本超强毒株 ９０１１ 相类似。５ 个分离株与现用疫苗株对比,至少在 Ｖ Ｐ２ 可变区内存在着一定的差异,这可能是 Ｉ Ｂ Ｄ Ｖ 接种免疫鸡群仍然暴发 Ｉ Ｂ Ｄ 的原因之一。 相似文献
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牛病毒性腹泻／粘膜病（Ｂｏｖｉｎｅｖｉｒａｌ ｄｉａｒｒｈｅａ／ｍｕｃｏｓａｌ ｄｉｓｅａｓｅ，ＢＶＤ／ＭＤ），简称牛病毒性腹泻（ＢＶＤ）或牛粘膜病（ＢＭ），是由牛病毒性腹泻／粘膜病病毒（ＢＶＤ／ＭＤＶ）感染牛引起的以发热、粘膜糜烂溃疡、白血胞减少、腹泻、咳嗽及怀孕母牛流产或产出畸形胎儿为主要特征的一种传染病。１９４６年Ｏｌａｆｓｏｎ等首次报道病毒性腹泻病。１９５３年Ｒａｍｓｅｙ和Ｃｈｉｖｅｒ发现粘膜病。１９６１年Ｇｉｌｌｅｓｐｉｅ等研究证明，这两种病毒是有共同抗原性的同种病毒，１９７１年由美国… 相似文献
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鸡传染性支气管炎病变型的研究进展 总被引：1，自引：0，他引：1
鸡传染性支气管炎（ＩＢ）是由传染性支气管炎病毒（ＩＢＶ）引起的急性、高度接触性传染病。目前世界上已分离的ＩＢＶ毒株达３０多种。由于新的变异毒株不断出现 ,致使病毒对组织器官亲嗜性发生变化 ,导致不同病变型的出现。本文就不同变异毒株引起的病变型进行综述 ,以供参考。１呼吸型为经典型ＩＢ ,首先由Ｓｃｈａｌｋ和Ｈａｗｎ（１９３１）在美国北部的Ｄａｋｏｔａ州发现。Ｂｅａｃｈ和Ｓｃｈａｌｍ（１９３６）确定该病由病毒引起。Ｊｕｎｇｈｅｒｒ等（１９５６）报道了分离株Ｃｏｎｎｅｃｔｉｃｕｔ和Ｍａｓｓａｃｈｕｓｅｔｔｓ… 相似文献
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应用杂交瘤技术获得了１３ 株抗传染性法氏囊病病毒（ＩＢＤＶ） 的单克隆抗体（ ＭｃＡｂ） ,经过检测其中有９ 株ＭｃＡｂｓ 为ＩｇＧ 类, ４ 株为Ｉｇ Ｍ , 这些ＭｃＡｂｓ 与新城疫病毒（ＮＤＶ） 、传染性支气管炎病毒 （ＩＢＶ） 和马立克氏病病毒（ ＭＤＶ） 均无交叉反应, 病毒中和试验表明, 有５株ＭｃＡｂｓ 具有中和活性,Ｗｅｓｔｅｒｎ － ｂｌｏｔｔｉｎｇ 试验表明, 这５ 株有中和活性的ＭｃＡｂｓ 识别的抗原位点在ＶＰ２ 。传染性法氏囊病最早于１９５７ 年发现于美国东部特拉华州的甘博罗（Ｇｕ ｍ ｂｏｒｏ） 地区,１９６２ 年由Ｃｏｓｇｒｏｖｅ 首次报道, 此后该病相继在全世界许多国家和地区广泛流行。本试验对通过淋巴细胞杂交瘤获得的１３ 株单克隆抗体进行了生物学特性鉴定, 以期应用于对ＩＢＤ 的研究, 现将试验情况报告如下。 相似文献
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SPSS／W7.5在畜牧场资料分类处理中的应用研究 总被引：1，自引：0，他引：1
ＳＰＳＳ（ＳｔａｔｉｓｔｉｃａｌＰａｃｋａｇｅｆｏｒｔｈｅＳｏｃｉａｌＳｃｉｅｎｃｅ，社会科学用软件包）是世界上著名的统计分析软件之一，它与ＳＡＳ（ＳｔａｔｉｓｔｉｃａｌａｎａｌｙｓｉｓＳｙｓｔｅｍ，统计分析系统），ＢＭＤＰ（ＢｉｏｍｅｄｅｃａｌＰｒ... 相似文献
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卵巢囊肿（Ｃｙｓｔｉｃ ｏｖａｒｉｅｓ）是奶牛的常见病,尤其是高产牛群发病率较高。卵巢囊肿可分为卵泡囊肿（Ｆｏｌｌｉｃｕｌａｒ ｃｙｓｔｓ）和黄体囊肿（Ｌｕｔｅａｌ ｃｙｓｔｓ）。前者是由于卵泡上皮变性、卵泡壁结缔组织增生变厚、卵泡液增多而形成;而黄体囊肿是由未排卵的卵泡上皮细胞黄体化而形成的。患卵泡囊肿的母牛表现发情异常,大多数病例发情周期变短,发情期延长,病牛哞叫、减食、追逐或爬跨其他牛,表现强烈的发情行为严重的成为慕雄狂,直肠检查卵巢上有波动的囊泡直径可达５～７厘米。患黄体囊肿的母牛主要表现… 相似文献
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猪伪狂犬病发生的新特点、发展趋势与防控对策 总被引：2，自引：0，他引：2
伪狂犬病（Ｐｓｅｕｄｏｒａｂｉｅｓ）又名奥捷士奇氏病（Ａｕｊｅｓｚｋｙ’ｓＤｉｓｅａｓｅ）,其病原为伪狂犬病毒（Ｐｓｅｕｄｏｒａｂｉｅｓ Ｖｉｒｕｓ,ＰＲＶ）属于疱疹病毒（Ｈｅｒｐｅｓｖｉｒｉｄａｅ）ａ疱疹病毒亚科（Ａｌｐｈａｈｅｒｐｅｓｒｉｒｉｎａｅ）,水痘病毒属（Ｖａｒｉｃｅｌｌｏ Ｖｉｒｕｓ）的猪疱疹病毒 Ｉ型（Ｐｏｒｃｉｎｅ ｈｅｒ－ｐｅｓｖｉｒｕｓ ＴｙｐｅＩ）。早在 １８１３年在美国的牛身上发现了一种叫“疯痒病”（“ｍａｄ ｉｔｃｈ”）的病原,１９０２年匈牙利兽医学家阿拉德奥捷士奇氏（Ａ… 相似文献
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猪囊尾蚴（Ｃｙｓｔｉｃｅｒｃｕｓｃｅｌｌｕｌｏｓａｅ）,又名猪囊虫,是存在于人体内猪带绦虫（Ｔａｅｎｉａｓｏｌｉｕｍ）的幼虫,主要寄生于猪体和人体,造成囊尾蚴病（Ｃｙｓｔｉｃｅｒｃｏｓｉｓ）。该病呈世界性分布,尤以发展中国家居多,我国是囊尾蚴病流行的国家之一,据目前不完全统计,在我国的１５个省、市、自治区均有囊尾蚴病流行,不仅给畜收稿日期：１９９９－０８－１８作者简介：高学军（１９６９－）,男,黑龙江阿城人,讲师,１９９０年毕业于山东大学生物化学专业,１９９８年毕业于东北农业大学兽医病理学专业,… 相似文献
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应加强对奶牛卵巢黄体化囊肿的认识徐立仁（南京农业大学动物医学院２１００９５）奶牛的卵巢囊肿（ｃｙｓｔｓｏｆｏｖａｒｙ）在许多人的概念中通常仅包括卵泡囊肿（ｃｙｓｔｓｏｆｆｏｌｉｃｌｅ）和黄体囊肿（ｃｙｓｔｓｏｆｌｕｔｅａ），而对于一种过渡形式的黄体化... 相似文献
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《Journal of aquatic animal health》2013,25(2):176-179
Abstract Lymphocystis was found in Caitipa mojarras Diapterus rhombeus collected in La Parguera, Puerto Rico. This is the first report of lymphocystis in the family Gerreidae. At four fish-cleaning sites frequently used by local fishers, 55% of the Caitipa mojarras were infected. Lymphocystis was not found in 100 Caitipa mojarras from four nearby locations which were identical except that they lacked fish-cleaning sites. All study sites had high levels of nitrification and petroleum hydrocarbons. Other fish species collected at these sites did not have gross signs of lymphocystis. Additional study is needed to determine if the high prevalence of lymphocystis at fish-cleaning sites is related to discarded fish tissue which could be infected with lymphocystis. 相似文献
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《Journal of aquatic animal health》2013,25(2):173-175
Abstract Lymphocystis occurred in Indian glassfish Chanda ranga imported to Puerto Rico from Thailand. This is only the second report of lymphocystis in Indian glassfish and in the family Chandidae (or Ambassidae), but the disease appears to have become widespread in this species in the ornamental aquarium fish trade throughout the southeastern USA and the Caribbean. We report the first occurrence of Indian glassfish with lymphocystis in a region where the fish can survive in the wild. Lymphocystis is not known in Puerto Rican fresh-water fishes, and the introduction of this disease should be avoided. 相似文献
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Cano I Alonso MC Garcia-Rosado E Saint-Jean SR Castro D Borrego JJ 《Veterinary microbiology》2006,113(1-2):137-141
An immunoblot technique for the detection of lymphocystis disease virus (LCDV) in naturally infected gilt-head seabream (Sparus aurata, L.) has been developed. A specific antiserum against a 60 kDa viral protein has been proven to be an appropriate tool for LCDV diagnosis either from inoculated cell cultures or from fish tissues using the immunoblot assay. The sensitivity of this technique varied between 10(-1) and 10(2) TCID50. LCDV has also been detected in fish tissues from both, diseased and asymptomatic gilt-head seabream. For the asymptomatic fish detection, a viral amplification step in cell culture and a subsequent viral concentration using polyethylene glycol (PEG) (600 wt) are required. On the contrary, immunoblot allowed the detection of LCDV antigens directly from tissue homogenates of diseased fish. The method described in this study shows higher sensitivity than classical detection techniques based on cell culture inoculation. 相似文献
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Six cases of ocular lymphocystis, a virus disease, are described. Lymphocystis is generally known as a benigh, unique, giant cell disease of fishes causing nodules on the skin and fins. It has been studied extensively because of the virus-host cell relationship that results in extreme size and lack of quick cellular destruction or stimulation to neoplasia. Lymphocystis cells were found behind or in one or both eyes and were also found on the cornea or adjacent skin surfaces. A retrobulbar mass produced extreme exophthalmos. Uveal (choroid and iris) masses were present in most cases. Optic nerve involvement was also seen. It is probable that the virus reached the eye by the blood with the resulting masses forming in situ rather than by direct extension from skin lesions. 相似文献
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《Journal of aquatic animal health》2013,25(3):231-235
Abstract This report documents natural infection and experimental transmission of lymphocystis in red drum Sciaenops ocellatus. Lymphocystis was a chronic problem in a commercial red drum production facility in Texas during 1993–1994. Electron microscopy of lesions and infected cell cultures revealed an icosohedral virus 220–230 nm in diameter. The virus was experimentally transmitted by intramuscular injection and oral administration, with the development of lesions restricted to fins and adjacent tissues. Cell culture of the virus yielded large numbers of incomplete particles, which might account for variations in experimental transmission of lymphocystis. 相似文献
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Atlantic salmon were selected from a fish farm with no previous record of pancreas disease (PD) or infectious pancreatic necrosis virus (IPNV) infection. Groups of fish were inoculated with either IPNV (strain Sp) from cell culture, organ material from fish with PD or control material as phosphate-buffered saline (PBS). Virological, histological and immunohistochemical examinations were carried out throughout the experiment. None of the fish died or showed clinical symptoms of PD. Histological examination revealed no pathological changes, and immunohistochemical studies were negative. Virus was isolated only sporadically from the group inoculated with organ material, whereas it was isolated consistently from the group inoculated with virus propagated in cell culture, as well as from in-contact control fish after the first week. In a latent carrier test, changes were entirely lacking in the first mentioned group, and were only slight in the last mentioned group. The data suggest that PD is not a transmissible disease, and that IPNV isolated from a PD outbreak does not play any part in the etiology of this disease. 相似文献
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《Journal of aquatic animal health》2013,25(3):236-240
Abstract Infectious salmon anemia (ISA) is a viral disease of Atlantic salmon Salmo salar that have been exposed to seawater in fish farms or hatcheries. This disease was previously believed to be exclusively one of salmon. However, it has been shown that anadromous brown trout Salmo trutta may carry the ISA virus (ISAV). Propagation of the ISAV in brown trout without the trout's showing any gross clinical signs of disease could be a result of a longstanding host-pathogen relationship between the virus and brown trout. A brown trout population isolated from the sea during the last 5,000 years and expected to be naive to the virus was challenged. These fish did not develop any gross signs of disease, but a few ISAVs were present as late as 46 d postchallenge. It was also shown that the ISA virus was present in brown trout as late as 7 months after challenge. 相似文献
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《Journal of aquatic animal health》2013,25(4):315-322
Abstract Largemouth bass virus (LMBV; family Iridoviridae) has recently emerged as a causative agent in fish kills of largemouth bass Micropterus salmoides. Little is known about how the virus is transmitted or what factors predispose fish to mortality subsequent to infection. Concern has nevertheless arisen that activities related to recreational angling may affect transmission dynamics and may alter the susceptibility of infected fish to clinical disease. This study examined the separate effects of two angling-related factors on the susceptibility of juvenile largemouth bass to mortality from LMBV infection and on the transmission of LMBV from infected to uninfected fish. The first factor was hook-and-line angling. Infected fish that underwent a simulated angling treatment did not experience higher mortality or have higher viral loads in their tissues than those that were not angled. The second factor was direct contact between infected and uninfected fish, as would occur in live wells and holding tanks. The LMBV was transmitted from infected to uninfected fish through water, even when direct contact was prevented. Transmission of LMBV between infected and uninfected fish separated by a fenestrated barrier was nearly as efficient as LMBV transmission between infected and uninfected fish that were allowed direct contact. These results imply that angling itself may have only minimal effects on the survival of largemouth bass infected with LMBV but that angling-related practices that place infected and uninfected fish together in a limited water volume may facilitate viral transmission. Partitioning or cooling of live wells and holding tanks, as well as limiting their use, could reduce LMBV-associated mortality and viral transmission. 相似文献
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Neil M Ruane Evelyn M Collins Michelle Geary David Swords Cathy Hickey Fiona Geoghegan 《Irish veterinary journal》2013,66(1):16