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1.
Mycobacterium bovis was isolated from 504 deer from 1970 to 1983. It was first isolated from feral red deer (Cervus elaphus) in New Zealand in 1970, and from farmed deer in 1978. Cervine tuberculosis has emerged as a significant problem in farmed deer and in 1983 M.bovis was found on 40 different farms. Thirty-five isolates of Mycobacterium avium-intracellulare have been cultured from deer but were associated with clinical disease in only four cases. Mycobacterium nonchromogenicum, Mycobacterium diernhoferi, Mycobacterium gastri, Mycobacterium chelonei, Mycobacterium smegmatis and Mycobacterium vaccae were isolated from deer but were not considered to be pathogenic.  相似文献   

2.
The aim of the present study was to investigate the prevalence of Trichinella infection in wolves (Canis lupus) in a 17,468 km2 area in Croatia. Muscle samples were collected from 67 wolves between 1996 and 2007 and analyzed by artificial digestion. Muscle larvae were detected in 21 wolves (31%) and genotyped by multiplex PCR. Trichinella britovi was the predominant species confirmed in 90% (19 wolves) while Trichinella spiralis was detected in 9% (2 wolves). The presence of the so called “domestic” Trichinella species was a surprise since, to date, only T. britovi had been reported in wild animals in this region. The larval burdens in infected animals ranged from 0.3 to 45.9 larvae per gram. The prevalence of infected animals varied by geographic region; infected animals were found in the region of Gorski Kotar (20%) which has very similar environment to the region of Lika, where almost all wolves were found infected. Interestingly, this is the first report of infected wolves in Dalmatia.  相似文献   

3.

Background

This study investigates Salmonella spp. isolated from privately kept reptiles and from environmental samples such as bedding materials or water from the floor of the enclosures (terraria). It also compares isolation of Salmonella using Modified Semisolid Rappaport-Vassiliadis (MSRV) medium or selective enrichment in Rappaport-Vassiliadis-Soya (RVS) pepton broth. Cloacal swabs or swabs from the cloacal area were collected from 63 individual reptiles belonging to 14 households. All reptiles were from different terraria and from 62 of these, environmental samples were also collected. Sampling were done by the reptile owners according to written instructions and sent by mail immediately after sampling. All but three samples were analyzed within 24 h after collection. Colonies suspected for Salmonella were tested for agglutination and serotyped using the White-Kauffmann-Le Minor scheme. The relative sensitivity (se) and specificity (sp) for MSRV compared with RVS, and the agreement coefficient kappa (κ) were calculated.

Results

Salmonella was isolated from 50/63 (80%) terraria, either from the reptiles (31/63; 49%) or from bedding material (39/62; 63%). The most common subspecies was Salmonella enterica subspecies enterica followed by S. enterica subspecies diarizonae. In reptiles, the most common S. enterica subspecies enterica serovars were Java (n = 4) and Fluntern (n = 4), compared with the serovars Tennessee (n = 10) and Fluntern (n = 10) in the environmental samples. The exact same set of Salmonella subspecies and serovars were not isolated from the individual reptiles and the environmental samples from any of the households. Isolation using MSRV yielded more Salmonella isolates 61/113 (54%) than enrichment in RVS 57/125 (46%). The se was 97.9% (95% Confidence Interval 93.9-100), the sp 78.5% (95% CI 68.5-88.5) and the κ 0.74, indicating substantial agreement between the tests.

Conclusions

Salmonella can be expected to be present in environments where reptiles are kept. This constitutes public health risks and should be considered during handling of the reptiles and during cleaning and disposal of bedding. A combination of different culturing techniques may be used to increase the isolation rate.  相似文献   

4.
SUMMARY A survey of antibiotic resistance in 1,287 strains of Salmonella from bovine, porcine and avian sources in Australia was carried out from 1975 to 1982. Isolates were tested against ampicillin, chloramphenicol, furazolidone, neomycin, streptomycin and tetracycline. Resistance was found to streptomycin in 286 isolates and to tetracycline in 282 isolates. Resistance to other antimicrobials was low and was unrelated to source. One hundred and seventy-three isolates showed multiple resistance to 2 or more antimicrobial agents with resistance to streptomycin and tetracycline being the most common. The overall level of resistance did not change over the period examined.  相似文献   

5.
从四川省雅安市某县12家长毛兔场的脱毛癣患兔中随机采样30份(1份/只);绵阳市某獭兔场脱毛癣患兔,采样10份(1份/只),经直接镜检、病原分离培养、致病性试验后,得到2种致病菌株。结合流行病学、临诊症状鉴定致病菌株为石膏样毛癣菌(Trichophyton mentagrophytes)和红色毛癣菌(Trichophyton rubrum)。在脱毛癣患兔中分离出红色毛癣菌,在国内尚属首次。文中同时提出了兔脱毛癣病防治对策。  相似文献   

6.
Trichinella spiralis and Trichinella britovi are species that are frequently found in domestic pigs and various sylvatic animals in Croatia. During routine trichinoscopy, non-encapsulated larvae were detected in the muscle tissue of a domestic pig. Artificial digestion revealed a larvae burden of 602 muscle larvae per gram of tissue. Tissue section analysis confirmed the presence of non-encapsulated larvae. Multiplex PCR identified the larvae as T. pseudospiralis. This observation is consistent with the reports of a local veterinary inspector who described the presence of non-encapsulated Trichinella in four individual cases over the last 2 years. This is the first report of T. pseudospiralis in Croatia and one of very few cases of T. pseudospiralis infection described in domestic pigs. The detection of non-encapsulated larvae stresses the need for implementation of artificial digestion instead of trichinoscopy for the detection and identification of Trichinella infections.  相似文献   

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Extract

Sir:- In June 1982, possums (Trichosurus vulpecula) from the Waitahuna forest area near Lawrence in Central Otago were examined for endoparasites (181 possums) and ectoparasites (125 possums). The alimentary tract was separated into stomach, small intestine and large intestine. These sections were washed thoroughly through a 60 mesh sieve and total parasite counts and identification were carried out. The fur from the base of the forelegs along the back and around the base of the tail was digested in 20% KOH and the ectoparasites were recovered. There was no evidence of pathogenicity ascribable to the parasites found. A summary of results is presented in Table I.  相似文献   

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12.
Bates C  Hiett KL  Stern NJ 《Avian diseases》2004,48(1):138-147
Campylobacter, a foodborne pathogen closely associated with poultry, is considered to be an important agent of human gastroenteritis in New Zealand. The pathways involved in the contamination of poultry flocks remain unclear; however, many vectors, such as insects, rodents, and wild birds, have been implicated. Infestation of poultry houses by insects, particularly darkling beetles (Alphitobius diaperinus), is difficult to control. Furthermore, darkling beetles are known vectors for a variety of pathogens that include Salmonella, infectious bursal disease virus, Aspergillus, Escherichia coli, and Marek's disease virus. In this investigation, the relationship between darkling beetles and Campylobacter contamination of poultry flocks was investigated. A New Zealand breeder flock and four of its progeny broiler flocks were included in the study. Samples of beetles and of intestinal excreta of the birds were cultured for the presence of Campylobacter spp. A subset of the recovered isolates was subsequently genotyped using flaA short variable region (SVR) DNA sequence analysis. A large number of Campylobacter subtypes were isolated, indicating that Campylobacter colonization of poultry is likely to arise from a number of different reservoirs. However, a set of genetically distinct isolates were found to be common to the broiler flocks and to the beetles. This research provides data that indicates that Alphitobius diaperinus may serve as a source of Campylobacter contamination of poultry. A more thorough understanding of the relationship between beetle infestation and the Campylobacter status of poultry flocks should enable progress in further development of biosecurity control measures.  相似文献   

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Experiences from BVDV control in Sweden   总被引:1,自引:0,他引:1  
Hult L  Lindberg A 《Preventive veterinary medicine》2005,72(1-2):143-8; discussion 215-9
A control scheme on BVDV was launched in Sweden in 1993 with the aim to prevent spread of the infection between herds, to eliminate virus from infected herds and, ultimately, to eradicate BVDV from Sweden. At the start it was voluntary and fully financed by the affiliated farmers. The estimated prevalence of infected herds was 40%. After 11 years there were only 205 (0.9%) herds still under clearance. Measures perceived to be of importance in concluding the scheme are subsidies on costs for analyses, gradually making regulations for contacts between herds and prevention of indirect infection stricter and also to eventually make the scheme compulsory.  相似文献   

15.
Cryptosporidiosis in chickens from southern Spain is reported. Cryptosporidia were found in the trachea, esophagus, and epithelium of the bursa of Fabricius in chickens from different regions. In one flock, cryptosporidiosis was associated with respiratory problems and high mortality. In another flock, it was associated with low mortality and weight loss.  相似文献   

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Renal dysfunction due to leptospirosis has been reported infrequently in the horse. The animals in this case presentation developed an acute and persistent febrile illness that could have been attributed to a number of infectious diseases. Upon numerous subsequent clinical evaluations, detection of renal dysfunction narrowed the list of differential diagnoses. This Case Report describes 3 horses with acute renal disease from Leptospira interrogans. Pyrexia was the initial clinical sign and was refractory to antimicrobial therapy. Once azotaemia developed, leptospirosis was diagnosed as the origin of the renal dysfunction. Treatment included i.v. crystalloid fluids, diuretics, anti‐inflammatory medication, antibiotics, pentoxifylline and polysulphated glucosaminoglycan. Additionally, one of these 3 horses required a continuous infusion of vasopressors to resolve the azotaemia. Pyrexia resolved within 48 h of treatment initiation for leptospirosis. The azotaemia resolved completely after 3 months (Case 1), 3 weeks (Case 2) and 5 days (Case 3).  相似文献   

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Borreliosis in dogs from southern Connecticut   总被引:13,自引:0,他引:13  
Blood samples were obtained from dogs in tick-infested regions of southern Connecticut to assess canine exposure to Borrelia burgdorferi, the etiologic agent of Lyme disease in human beings. An indirect fluorescent antibody test detected immunoglobulin (Ig)M antibodies at titers of 1:64 to 1:512 in 22 of 84 serum samples previously shown to be positive with a polyvalent rabbit anti-dog total Ig conjugate. Analyses of paired serum samples from 20 seropositive dogs revealed temporal differences in titers; changes occurred during brief (1 month) or extended (greater than 4 years) sampling periods. Clinical records for 52 seropositive dogs indicated a history of intermittent lameness in 19 of these. Limb/joint disorders typically developed in dogs without IgM antibodies, suggesting manifestation during later phases of illness. A microscopic-agglutination test was used to assess cross reactivity between B burgdorferi and 20 serovars of Leptospira interrogans and biflexa. Analyses of 63 dog serum specimens with antibodies to B burgdorferi and a series of reference rabbit sera revealed minor antigenic relatedness. There was geographic clustering of dogs with antibodies to B burgdorferi in areas of south-central and southeastern Connecticut, where human Lyme disease also occurs.  相似文献   

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