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1.
Varying levels of lupin (Lupinus albus), peas (Pisum sativum) and rapeseed (Brassica napus) meals were evaluated as partial replacements for fishmeal in extruded diets for rainbow trout, with particular emphasis on the effect on growth performance and the expression of three genes associated with immune response. A series of 10 isonitrogenous (450 g kg?1 crude protein) and isolipidic (17 g kg?1 crude lipid) diets were formulated to contain different levels of lupin (150 g kg?1, 250 g kg?1 and 350 g kg?1), rapeseed cake (100 g kg?1, 200 g kg?1 and 300 g kg?1) and pea (50 g kg?1, 150 g kg?1 and 250 g kg?1) meals. The control diet was prepared with fish meal as the sole source of protein. Triplicate groups of fish (37.08 ± 3.58 g) were assigned to each experimental diet. The feeding experiment was conducted for 9 weeks at 14.3 ± 0.4 °C. The fish were hand fed three times per day, 6 days per week to apparent satiation level. Growth performance, feed utilization and immunological response were significantly affected by the type of plant protein as well as level of inclusion. Hepatosomatic index (HSI) increased in all groups of fish fed diets with pea and rapeseed cake meal. Dietary inclusion of lupin did not affect the expression of Mx‐1 gene. Our results suggest that fish meal can be replaced by lupin in the diet of rainbow trout without any apparent adverse effects on key innate immunological genes.  相似文献   

2.
The present study investigated the optimum dietary protein level for the maturation of adult Pangasianodon hypophthalmus broodstock. Four isocaloric diets containing 250, 300, 350 and 400 g kg?1 of protein levels were prepared and presented to triplicate groups of fish. The fish (mean weight 770 ± 17.23 g and 712 ± 23.42 g for females and males respectively) were stocked in outdoor canvas tanks (4 m × 1 m × 1 m) at a stocking density of 20 fish/tank with a male: female ratio of 1:4. The fish were fed the test diets to satiation twice daily for 6 months. Gonadosomatic index (GSI) and fecundity were similar among fish fed dietary protein levels, higher than those fed on the 250 g kg?1. Final weight, weight gain, oocyte weight were significantly highest (< 0.05) for the fish fed 350 and 400 g kg?1 dietary protein treatments. Only the 350 g kg?1 dietary protein treatment resulted in significantly best ovipositor diameter and % ripe egg. Amino acid levels were highest in the muscle followed by the oocyte and liver of fish fed 350 and 400 g kg?1 dietary protein levels. The present results suggested that a dietary protein level of 350 g kg?1 can be recommended for the development of P. hypophthalmus broodstock.  相似文献   

3.
In experiment 1, juvenile sea urchins (n = 80, 0.088 ± 0.001 g wet weight and 5.72 ± 0.04 mm diameter) were held individually and fed ad libitum one of three semi‐purified formulated diets (n = 16 individuals treatment?1). In the diets, protein was held constant (310 g kg?1 dry, as fed) and carbohydrate level varied (190, 260, or 380 g kg?1 dry, as fed). Wet weights were measured every 2 weeks. Total wet weight gain was inversely proportional to dietary carbohydrate level and energy content of the respective diet. In experiment 2, sea urchins (5.60 ± 0.48 g wet weight, n = 40) fed 190 g kg?1 carbohydrate consumed significantly more dry feed than those fed 260 g kg?1, but not more than those fed 380 g kg?1 carbohydrate. Based on differential feed intake rates, sea urchins that consumed more feed also consumed higher levels of protein and had the highest weight gain. Consequently, protein content and/or protein: energy ratio may be important in determining feed utilization and growth among sea urchins in this study. The average digestible energy intake was approximately 70 kcal kg?1 body weight day?1, suggesting daily caloric intake of juvenile Lytechinus variegatus is lower than in shrimp and fish.  相似文献   

4.
An 8‐week feeding trial was conducted to assess dietary protein and lipid levels on growth performance, feed utilization and body composition of juvenile red‐spotted grouper (7.85 ± 0.03 g fish?1). Nine semi‐purified diets were formulated containing varying protein levels (440–520 g kg?1, dry matter) and lipid levels (60–120 g kg?1, dry matter). The weight gain of juvenile Epinephelus akaara was affected by dietary protein (= .005) and its interaction with dietary lipid (= .020). Viscerosomatic index, intraperitoneal fat ratio and whole‐body lipid level increased with increasing dietary lipid level (p < .001). Nitrogen retention was not affected by dietary protein and lipid, while lipid retention decreased with increasing dietary lipid level (p < .001). The plasma blood urea nitrogen increased with increasing dietary protein level (= .003). This study showed that diet with 520 g kg?1 protein and 60 g kg?1 lipid with 30.58 mg kJ?1 P:E provided a maximal growth for this species. Moreover, an increase in dietary lipid levels (from 60 to 90 g kg?1) could reduce the protein requirement (from 520 to 480 g kg?1) without affecting the growth performance, while higher fat deposition was observed in fish fed high‐lipid diets.  相似文献   

5.
W. Li  X. Wen  Y. Huang  J. Zhao  S. Li  D. Zhu 《Aquaculture Nutrition》2017,23(5):1035-1047
A two‐factor experiment was designed to determine the suitable dietary protein and lipid levels for juvenile Nibea diacanthus. Nine extruded pellet diets were formulated to contain three levels of protein (420, 470 and 520 g kg?1) and three levels of lipid (70, 110 and 150 g kg?1). Each diet was randomly fed to triplicate groups of 25 juvenile N. diacanthus (initial weight 12.12 ± 0.23 g) for 8 weeks in net cages. The results showed that weight gain rate (WGR), specific growth rate, final body weight and energy retention were significantly influenced by the dietary protein and lipid levels‐. The highest WGR (982.5g kg?1.) of N. diacanthus was found in the group with dietary protein and lipid of 520 and 150 g kg?1. Feed intake and feed efficiency were significantly impacted by the dietary protein levels. An interactive effect between dietary protein and lipid on the protein retention and protein efficiency ratio was observed. There were no significant differences in condition factor and survival among all treatments. Hepatosomatic index and viscerasomatic index of N. diacanthus were positively related with dietary lipid levels, but negatively with dietary protein levels. Crude protein, crude lipid, ash, moisture and energy contents of the whole body, muscle and liver were influenced by dietary protein and lipid levels. Moreover, total essential amino acid pattern of the muscle was correlated to those of dietary protein. Total protein concentration in the serum was affected by dietary protein and lipid levels. Meanwhile, both serum cholesterol and triglyceride concentrations increased with increasing dietary lipid levels. These results demonstrate that the diet containing 470 g kg?1 protein and 110 g kg?1 lipid is optimal for juvenile N. diacanthus and analysis of WGR by quadratic regression indicated that the estimated optimal protein‐to‐energy ratio for juvenile N. diacanthus was 24.53 mg protein kJ?1.  相似文献   

6.
Four semi‐moist formulated feeds were supplied to Octopus vulgaris subadults (664 ± 70 g; 18.4 ± 0.7°C) in two different experiments. In the experiment #1, two diets were prepared with a new mixture of binders (gelatine 150 g kg?1, starch 100 g kg?1 and gum 50 g kg?1). The GEL15‐Squid and GEL15‐Hake feeds included 100 g kg?1 freeze‐dried squid (Todarodes sagittatus) or hake (Merluccius sp.) respectively. Both feeds showed low water disintegration rates at 24 h (13.6–15.0% dry weight). The specific feeding rate was higher in animals fed GEL15‐Hake (2.7%BW day?1), but the growth and feed efficiency were significantly better in animals fed GEL15‐Squid (1.4%BW day?1 and 61.2% respectively; P < 0.05). The proximate composition of the digestive gland, carcass and whole animals was similar. In the experiment #2, the GEL15‐Squid amino acid profile was improved by replacing 50 g kg?1 gelatine by 50 g kg?1 freeze‐dried squid (GEL10‐Squid) or freeze‐dried fish (GEL10‐Fish). These feeds showed higher water disintegration rates (31.7–36.3% dry weight). The feeding rates (2.2–2.3%BW day?1), growth (1.5%BW day?1) and feed efficiency were similar for both diets. Total lipids were higher in the digestive gland and whole animals fed GEL10‐Fish diet (P < 0.05). Future effort could be directed towards alternative binders that allow improve amino acid balance with a minimum gelatine content or even supplementation trials including essential amino acids.  相似文献   

7.
A 14 weeks growth trial was performed to estimate the protein requirement for growth and maintenance of zebra sea bream (Diplodus cervinus) juveniles. For that purpose, nine isolipidic diets were formulated to contain increasing protein levels (from 50 to 550 g kg?1) at the expense of carbohydrate. Each diet was assigned to duplicate groups of 20 fish, with an average body weight of 7.7 g. Feed efficiency improved with dietary protein up to 400 g kg?1, no further differences being noticed at higher protein levels. Fish fed the 50 g kg?1 protein diet lost weight during the trial. In the other groups, weight gain improved as dietary protein increased up to 350–400 g kg?1. Fish fed diets with 250 g kg?1 protein or lower had lower whole‐body protein content than the other groups. A curvilinear‐plateau model was used to adjust weight gain and protein gain (g kg ABW?1 day?1) to dietary protein levels. Based on that model, the optimum dietary protein requirement for maximum weight gain was estimated to be 437.6 g kg?1 and for maximum protein gain 461.9 g kg?1, corresponding to a protein intake of 7.63 g kg ABW?1 day?1. Protein requirement for maintenance was estimated to be 1.01 g kg ABW?1 day?1.  相似文献   

8.
The objectives of the present study were to investigate of GroBiotic®‐A (GBA) on growth, autochthonous intestinal microbiota and haemato‐serological parameters of beluga juvenile. A total of 180 fish (40.82 ± 5.81 g) were fed diets containing graded levels of GBA (0, 5, 10 and 20 g kg?1 diet) for 8 weeks. No significantly differences in body composition, total viable aerobic bacteria, mean cell volume, mean cell haemoglobin, mean cell haemoglobin concentration, neutrophils, eosinophils, monocytes, albumin, glucose, triglyceride, cholesterol, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase and lactate dehydrogenase were detected between treatment groups. The fish fed on 10 and 20 g kg?1 GBA significantly showed higher lactic acid bacteria, final weight, body weight increases, weight gain and lower feed conversion ratio compared with the control and 5 g kg?1 groups. The group fed 20 g kg?1 GBA showed a highly significant difference in condition factor, survival rate, final length, total red and white blood cells, lymphocytes, haematocrit, haemoglobin, total protein, total immunoglobulin and lysozyme activity. The specific growth rate of the treatment groups was significantly elevated compared with the control groups. These results indicated that GBA at level 20 g kg?1 improved growth, welfare and survival of beluga juvenile.  相似文献   

9.
Threonine is the third‐limiting essential amino acid in diets based on cereal ingredients. A 4‐week trial was conducted to determine the threonine requirement of large Nile tilapia based on fish growth, feed efficiency, body composition, protein and amino acid retention. Six hundred fish (563.3 ± 15.1 g) were distributed into twenty 1.2‐m3 cages. Five diets containing 288 g kg?1 of crude protein, 12.7 MJ kg?1 of digestible energy and 8.9, 10.5, 12.2, 13.7 and 15.4 g kg?1 of threonine were elaborated. Fish were hand‐fed five times a day to extruded diets. Significantly, differences in growth performance and amino acids retention among the treatments were observed. Fish fed 10.5 g kg?1 of threonine showed higher daily weight gain, gutted weight and fillet weight (P < 0.05) compared to fish fed with other experimental diets. Diets containing 8.9–15.4 g kg?1 of threonine did not affect whole body and muscle proximate composition. Based on second‐order regression analysis, the dietary threonine requirement estimated based on final gain, fillet weight and fillet yield was 12, 12.1 and 11.5 g kg?1 diet, respectively. The dietary threonine requirement for maximum fillet yield of Nile tilapia was estimated to be 11.5 g kg?1.  相似文献   

10.
A feeding trial was conducted to study the effect of dietary lipid on growth performance and heat‐shock protein (HSP70 and HSP60) response of white seabass (WSB), Atractoscion nobilis. Five diets were formulated to contain 440 g kg?1 protein from 300 g kg?1 fish meal, 240 g kg?1 soybean meal and 100 g kg?1 soy protein concentrate with different levels of lipid: 100, 120, 140, 160 or 180 g kg?1. At the end of the trial, heat shock response based on HSP70 and HSP60 was measured in liver and white muscle from fish at ambient temperature and temperature shock conditions. Final weight and percent gain were significantly higher for fish fed the 100 g kg?1 lipid diet than for fish fed the rest of the diets (P ≤ 0.05). Feed conversion ratio was lowest for fish fed the 100 g kg?1 lipid diet. The HSP70 and HSP60 responses were positively correlated to dietary lipid levels following temperature shock. At ambient temperature, HSP60 and HSP70 responses in muscle and HSP60 response in liver increased with dietary lipid level. Temperature shock significantly increased the HSP response of fish in all treatments. Results of this study demonstrated that a moderate (110–120 g kg?1) level of dietary lipids would be recommended for production diets but a higher dietary lipid level may be required for optimal stress tolerance.  相似文献   

11.
Twelve experimental diets (D‐1 to D‐12) in a 4 × 3 factorial design (four protein levels: 250, 350, 400 and 450 g kg?1 and three lipid levels: 50, 100 and 150 g kg?1) were formulated. Carbohydrate level was constant at 250 g kg?1. Rohu fingerlings (average wt. 4.3 ± 0.02 g) were fed the experimental diets for 60 days in three replicates at 2% BW  day?1. Weight gain (%), specific growth rate (% day?1) and feed gain ratio (FGR) indicated that diets containing 450 g kg?1 protein and 100 or 150 g kg?1 lipid (diets D‐11 and D‐12) resulted in best performance, although results were not significantly different from those of diet D‐9 (400 g kg?1 protein and 150 g kg?1 lipid). Protein efficiency ratio was highest with diets D‐6 (350 g kg?1 protein and 150 g kg?1 lipid) and D‐9 (400 g kg?1 protein and 150 g kg?1 lipid) (P > 0.05) and declined with higher and lower protein diets at all levels of lipid tested. Elevated lipid level (50, 100 or 150 g kg?1) did not produce better FGR in diets containing 400 and 450 g kg?1 dietary protein (P > 0.05). The combined effects of protein and lipid were evident up to 400 g kg?1 protein. Growth and FGR showed consistent improvement with increased lipid levels from 50 to 150 g kg?1 at each protein level tested except with diets containing 450 g kg?1 protein. Apparent nutrient digestibility (for protein, lipid and energy) did not show significant variation among different dietary groups (P > 0.05). Whole body protein and lipid contents increased significantly (P > 0.05) with dietary protein level. The results of this study indicate that rohu fingerlings are adapted to utilize high protein in diets with varying efficiency. The fish could utilize lipid to spare protein but there is no significant advantage from this beyond the dietary protein level of 350–400 g kg?1 in terms of growth and body composition.  相似文献   

12.
A growth trial was conducted to estimate the optimum concentration of dietary potassium (K) for grass carp (Ctenopharyngodon idella). Triplicate groups of grass carp (3.96 ± 0.06 g) were fed diets containing graded levels (0.87, 2.90, 5.37, 7.54, 9.87 and 12.4 g kg?1) of K for 8 weeks. Final body weight, weight gain and feed efficiency and gill Na+‐K+ ATPase activity were highest in fish fed with 9.87 g kg?1 dietary K and lowest in fish fed the basal diet (P < 0.05). The K contents in whole body and muscle were linearly increased up to the 9.87 g kg?1 dietary K and then levelled off beyond this level, whereas in scales and vertebrae up to the 7.54 g kg?1 dietary K (P < 0.05). However, dietary K levels had no significant effect on ash, Ca, P and Mg contents in whole body, scales, vertebrae or muscle. Analysis using polynomial regression of weight gain and gill Na+‐K+ ATPase activity and using the broken‐line regression of whole body K concentrations indicated that the adequate dietary K concentration for grass carp is about 9.45–9.99 g kg?1 diet.  相似文献   

13.
Feed requirements were estimated from specific growth rates in standardized soft tissue dry weight (SGRDW) and atomic O:N ratios for mussels fed seven rations of microalgae (5–735 μg C h?1 ind?1) at 7 and 14°C respectively. The mean oxygen consumption and ammonia‐N excretion rates were significantly higher at 14°C (0.29 μg O2 and 27.3 μg N ind?1 h1) compared with those at 7°C (0.16 μg O2 and 11.4 μg N ind?1 h?1) (P < 0.05), resulting in O:N ratios between 3 and 45 at 7°C and 7 and 28 at 14°C. Low O:N ratios indicate protein catabolism and an unfavourable condition, whereas high ratios indicate that carbohydrate is the primary energy source. The measured SGRDW suggests minimum feed requirements of ~240 and ~570 μg C ind?1 h?1 for weight maintenance at 7 and 14°C, with corresponding O:N ratios of 24 and 16, respectively, indicating a more stressed condition at 14°C. A 0.5% SGRDW day?1 was obtained by ~565 (O:N = 29) and ~680 (O:N = 23) μg C ind?1 h?1 at 7 and 14°C respectively. A positive and significantly higher SGRDW, with the lowest feed ration at 7°C compared with a negative SGRDW at 14°C (P < 0.05), indicated that storage time can also possibly be prolonged at low temperatures if the mussels are not fed.  相似文献   

14.
Six isonitrogenous and isocaloric semi‐purified diets were prepared with different levels of microbial levan: control (Basal), T1 (Basal + 2.5 g kg?1 diet), T2 (Basal + 5 g kg?1 diet), T3 (Basal + 7.5 g kg?1 diet), T4 (Basal + 10 g kg?1 diet) and T5 (Basal + 12.5 g kg?1 diet), fed to six groups of fish in triplicate tanks. The results of the 60 days feeding trail showed that supplementation of dietary levan significantly affected the weight gain percentage and specific growth rate of the treatment groups fed at 10 g kg?1 or more levan. Lowest feed conversion ratio (FCR) value and highest survival percentage among levan fed groups were observed with 12.5 g kg?1 incorporation (T5) and was comparable with (T4) group. Significant increase in muscle RNA level and RNA/DNA ratio was observed with the increasing dietary levan. Fish fed 12.5 g kg?1 levan had significantly higher protease, amylase and lipase activities compare with the control group. Lowest Aspartate aminotransferase (AST) activity in the liver and muscle was observed in the T5 group fed with highest level of dietary levan. Overall results conclude that dietary microbial levan incorporation at 12.5 g kg?1 could be used as potent dietary prebiotic for the culture of L. rohita juveniles.  相似文献   

15.
An 8‐week feeding trial was conducted to investigate the effects of different taurine levels on the growth performance of juvenile white shrimp fed with low‐fishmeal diets. Six level diets of dietary taurine were prepared by the supplementation of taurine (0, 0.4 g kg?1, 0.8 g kg?1, 1.2 g kg?1, 2.0 g kg?1 and 4.0 g kg?1) to a control diet (100 g kg?1 fish meal). Each diet was randomly assigned to triplicate groups of 30 shrimps (0.48 ± 0.0 g), each three times daily. Shrimp fed the 0.4 g kg?1 and 0.8 g kg?1 taurine‐supplemented diets, showed significantly higher weight gain, protein efficiency ratio and protein retention efficiency than those of shrimp fed the other diets. The quadratic regression analysis (y = ?55.59x2 + 187.1x + 750.2 R² = 0.587) indicated that a maximum weight gain occurring at 1.68 g kg?1 of taurine level. The whole body and hepatopancreas taurine contents of the taurine‐supplemented diets were on the same level and higher than those of the control group. Total free amino acid content in the hepatopancreas was significantly affected by taurine supplementation. The results of the present study demonstrate that the white shrimps require taurine as an essential nutrient for growth performance.  相似文献   

16.
A 10‐week feeding trial with four dietary protein levels (400, 450, 500 and 550 g kg?1 crude protein) and two dietary lipid levels (80 and 160 g kg?1 crude lipid) was conducted to assess optimum dietary protein and lipid levels for the growth, feed utilization and body composition of juvenile Manchurian trout (initial weight 11.80 ± 0.15 g). Fish were fed twice daily (08:30 and 16:30 h) to apparent satiation. The results showed that fish fed the diet with 500 g kg?1 protein and 80 g kg?1 lipid had the highest growth and feed efficiency. However, fish fed the diet with 450 g kg?1 protein and 160 g kg?1 lipid showed comparable growth to that of the fish fed diet 5 (500/80) and had higher protein efficiency ratio (PER), nitrogen retention (NR) and energy retention (ER) than other groups (< 0.05). Growth, PER, NR and ER of fish fed the 160 g kg?1 lipid diet was significantly higher (< 0.05) than that of fish fed the 80 g kg?1 lipid diet at 400 and 450 g kg?1 protein diet, whereas these values showed an opposite trend at 500 and 550 g kg?1 protein diet, and the lowest PER, NR and ER was found by fish fed the 400 g kg?1 protein diet with 80 g kg?1 lipid. Fish fed diets with 400 g kg?1 protein had lower feed intake (FI) than that of other groups. Feed intake of fish fed 80 g kg?1 lipid level was significantly lower than that of fish fed 160 g kg?1 lipid diet at 400 g kg?1 protein (< 0.05), while no significant differences were observed at 450, 500 and 550 g kg?1 protein‐based diets. Contrary to moisture content, lipid content of whole body and muscle increased significantly (< 0.05) with increasing lipid levels. The results of this study indicated that the diet containing 450 g kg?1 protein and 160 g kg?1 lipid, with a P/E ratio of 23.68 g protein MJ?1 would be suitable for better growth and feed utilization of juvenile Manchurian trout under the experimental conditions and design level used in this study.  相似文献   

17.
The aim of the present work was to obtain the lipid utilization of Octopus vulgaris supplying formulated semi‐moist diets with different contents in cod oil (reduced from water content): 0 g kg?1 (A0, 138 g kg?1 lipids DW; N = 4), 100 g kg?1 (A100, 286 g kg?1 lipids DW; N = 6) and 200 g kg?1 (A200, 388 g kg?1 lipids DW; N = 6). The rest of the ingredients were constant in the three diets: 200 g kg?1 gelatin, 100 g kg?1 egg yolk powder, 150 g kg?1 freeze‐dried Todarodes sagittatus and 50 g kg?1 freeze‐dried Sardinella aurita). Survival was 100% with the three diets. The highest absolute feeding (15.8 ± 1.2 g day?1), growth (9.6 ± 1.4 g day?1; 0.91% BW day?1) and feed efficiency rates (60.3%) were obtained with diet A0. This diet also showed greater retention of lipid and protein than A100 and A200. Protein digestibility was above 95% in all of the diets. Only diet A0 led to a high lipid digestibility coefficient (81.25%), which fell drastically to 12.3% in A200. It was notable the high polar lipid digestibility rates (83–89%) respect to neutral lipids (2–87%) in all diets. The best results were obtained with lipid feeding rates of around 1 g day?1 and a suitable lipid content on 130–140 g kg?1 DW in formulated diets for O. vulgaris.  相似文献   

18.
This study evaluated the effects of soy protein ratio, lipid content and the minimum dietary level of krill meal in plant‐based diets over the growth performance and digestibility of Litopenaeus vannamei. Nine plant‐based diets varied the soybean meal (SBM) and soy protein concentrate (SPC) inclusion ratio at 1 : 2.3, 1 : 1 and 2.5 : 1, and their dietary lipid content at 121.4 ± 9.4, 102.3 ± 1.2, and 79.9 ± 1.2 g kg?1 (in a dry matter basis). An additional diet containing 120 g kg?1 of fish meal (salmon by‐product) was used as a control. Krill meal was included at 0, 5, 10, 20 and 30 g kg?1 in a new set of plant‐based diets. After 10 weeks in clear‐water tanks of 0.5 m3, no effect of SBM:SPC ratio and dietary lipid content was detected on shrimp survival. However, dietary lipid levels of 80 and 121 g kg?1 combined with a high SPC to SBM resulted in the lowest final body weight and the poorest apparent crude protein digestibility, respectively. Krill meal increased feed intake at only 10 g kg?1, while at 20 g kg?1, it accelerated shrimp growth, increased yield and reduced food conversion ratio.  相似文献   

19.
Growth, feed efficiency and proximate and lipid class composition of subadults Octopus vulgaris (788 ± 133 g; 18.5°C) fed formulated diets of low lipid (LL: 8 g kg?1) and high lipid levels (HL: 84 g kg?1) and each one of these with three different levels of glutamate supplementation (0, 5 and 20 g kg?1) were compared. All the animals accepted the diets with a survival of 100%. The addition of glutamate did not stimulate feeding rates in any of the assays (2.48–2.64 and 1.86–2.01%Body weight day?1 for LL and HL, respectively; P > 0.05). The best growth, feed efficiency and protein productive value were observed in the groups fed 5 or 20 g kg?1 glutamate supplementation at both lipid levels, with significant differences for LL diet (P < 0.05). A better feed efficiency was achieved with the HL diet (14.6–27.5% vs. 2.5–19.2% for LL diet). There were no significant differences in the proximate composition of carcass (animal excluding the digestive gland). However, a substantial amount of lipids accumulated in the digestive gland, mainly triglycerides, was detected as a consequence of higher lipid ingestion or glutamate supplementation. It was notable the lower percentages of phosphatidylethanolamine and phosphatidylcholine in the digestive gland of animals with best growth.  相似文献   

20.
A 56‐d feeding trial was conducted to investigate the effect of dietary mannan‐oligosaccharides (MOS) and fructo‐oligosaccharide (FOS) on growth indices, body composition, intestinal bacterial community and digestive enzymes activity of regal peacock. A total of 240 fish were randomly distributed to 15 experimental units (40‐L aquariums) of 16 fish each. These replicates were randomly assigned to one of five treatments in a 2 × 2 + 1 factorial arrangement. The treatments were control diet (no MOS and FOS), diet A (2 gkg?1 MOS + 1.5 g kg?1 FOS), diet B (2 g kg?1 MOS + 3 g kg?1 FOS), diet C (4 g kg?1 MOS + 1.5 g kg?1 FOS) or diet D (4 g kg?1 MOS + 3 g kg?1 FOS). The results showed that feeding diet C increased specific growth rate and protein efficiency ratio and decreased feed conversion ratio compared with control diet. Higher intestinal trypsin activity and increased Lactobacillus counts were observed in fish fed diets B and C. All diets significantly elevated body protein deposition and intestinal amylase activity compared to the control diet. In conclusion, the diet supplemented with 4 g kg?1 MOS + 1.5 g kg?1 FOS was advantageous over other MOS + FOS‐supplemented diets, with respect to growth performance and health benefits of regal peacock.  相似文献   

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