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1.
Two hundred and forty 1‐day‐old male Arbor Acres broiler chickens were randomly assigned to five dietary treatments with six replicates of eight chickens per replicate cage for a 42‐day feeding trial. Broiler chickens were fed a basal diet supplemented with 0 (control), 250, 500, 750 or 1000 mg/kg betaine, respectively. Growth performance was not affected by betaine. Incremental levels of betaine decreased the absolute and relative weight of abdominal fat (linear P < 0.05, quadratic P < 0.01), low‐density lipoprotein cholesterol (LDL‐C), triglyceride (TG) and total cholesterol (TC) (linear P < 0.05), and increased concentration of nonesterified fatty acid (NEFA) (linear P = 0.038, quadratic P = 0.003) in serum of broilers. Moreover, incremental levels of betaine increased linearly (P < 0.05) the proliferator‐activated receptor alpha (PPARα), the carnitine palmitoyl transferase‐I (CPT‐I) and 3‐hydroxyacyl‐coenzyme A dehydrogenase (HADH) messenger RNA (mRNA) expression, but decreased linearly (P < 0.05) the fatty acid synthase (FAS) and 3‐hydroxyl‐3‐methylglutaryl‐CoA (HMGR) mRNA expression in liver of broilers. In conclusion, this study indicated that betaine supplementation did not affect growth performance of broilers, but was effective in reducing abdominal fat deposition in a dose‐dependent manner, which was probably caused by combinations of a decrease in fatty acid synthesis and an increase in β‐oxidation.  相似文献   

2.
Various lines of evidence suggest that appetite‐related neuropeptides in the hypothalamus are regulated by adiposity signals such as leptin and insulin in mammals. In the present study, we examined age‐dependent changes in the weight of abdominal fat and hypothalamic mRNA levels of neuropeptide Y (NPY, an orexigenic neuropeptide) and proopiomelanocortin (POMC, a precursor of anorexigenic neuropeptides) in growing chickens at 7, 14, 21 and 28 days of age. Hypothalamic NPY mRNA levels were significantly (P < 0.05) decreased after 14 days of age, whereas hypothalamic POMC mRNA levels were significantly (P < 0.05) increased at 28 days of age. The percentage of abdominal fat was significantly increased after 14 days of age in chickens. We next examined the correlation of hypothalamic NPY and POMC mRNA levels and several parameters at 28 days of age. There were no significant correlations between hypothalamic mRNA levels of NPY or POMC and the percentage of abdominal fat. These findings suggest that the gene expressions of NPY and POMC do not depend on adiposity in chickens, at least in 28‐day‐old layer chickens.  相似文献   

3.
This research was conducted to study the performance and carcass parameters of broiler chickens fed diets supplemented with heat‐treated non‐starch polysaccharide degrading enzyme. A total of 432 one‐day old Ross 308 broiler chickens were allocated to five treatments: (i) CON (basal diet), (ii) E1: CON + 0.05% multi‐enzyme, (iii) E2: CON + 0.1% multi‐enzyme, (iv) E3: CON + 0.05% thermo‐resistant multi‐enzyme and (v) E4: CON + 0.1% thermo‐resistant multi‐enzyme, each treatment consisted of six replications and 12 chickens in each replication. The chickens were housed in three floor battery cages during 28‐day experimental period. On days 1–7, gain in body weight (BWG) improved by feeding the diets supplemented with thermo‐resistant multi‐enzyme. On days 7–21 and 1–28, chickens fed the diets containing thermo‐resistant multi‐enzyme showed improved (p < 0.05) BWG and feed conversion ratio (FCR) compared to CON group. Supplementing the diets with multi‐enzyme or thermo‐resistant multi‐enzyme affected the percentage of drip loss on d 1 (p < 0.05). Drip loss percentage on days 3 and 5 and also meat colour were not affected significantly. Supplementing the diets with multi‐enzyme or thermo‐resistant multi‐enzyme did not affect the relative weights of organs but compared to CON group, relative weight of breast muscle increased and abdominal fat decreased (p < 0.05). Among measured blood constituents, chickens fed supplemented diets with thermo‐resistant multi‐enzyme showed higher (p < 0.05) IgG. Counts of red and white blood cells and lymphocyte percentage were not affected. In conclusion, the results demonstrated that supplementing pelleted diets with thermo‐resistant multi‐enzyme improved performance of broiler chickens.  相似文献   

4.
本研究旨在考察持续高温对不同性别北京油鸡生长性能、肉质性状及脂肪沉积的影响.选择健康、体况相近的49日龄北京油鸡公母各72只(公鸡平均体质量(608.58±13.07)g;母鸡平均体质量(518.50±8.33)g)随机分在2个处理中:(1)高温组,(35±0.37)℃;(2)适温组,(25±0.38)℃,分别在2个人工环境气候舱中饲养.每个处理设6个重复,每个重复6只鸡.试验持续23 d.测定试验第1~11、12~22和1~22天的生产性能指标及第12和23天时的胴体性状、肉质性状及脂肪沉积率.结果表明;1)热暴露1~11 d,公母鸡的日增体质量都显著降低(P<0.01),料重比无明显变化(P>0.05).热暴露12~22 d,公鸡的日增体质量显著降低(P<0.01),而母鸡无明显变化(P>0.05),同时,公鸡的料重比显著提高(P<0.01);2)与适温组相比,高温组各性别北京油鸡的肉质性状均无明显变化(P>0.05);3)对公鸡而言,热暴露12 d时腹脂、肌间脂肪和皮下脂肪沉积率及胴体率、腿比率与适温组相比均无显著差异(P>0.05).热暴露23 d时各部位脂肪沉积率均显著降低(P<0.01),而胴体率和腿比率显著提高(P<0.05);对母鸡而言,热暴露12d时腹脂、肌间脂肪沉积率显著降低(P<0.05),热暴露23 d时皮下脂肪率显著降低(P<0.05),腹脂、肌间脂肪沉积率及胴体率、腿比率与适温组相比均无显著差异(P>0.05).结果提示,热暴露12 d,各性别油鸡的日增体质量均显著降低,随着热暴露进一步延续,公鸡日增体质量的降低幅度加大,而母鸡的已无明显变化;随着热暴露时间的延长,公鸡的脂肪沉积逐渐下降,而母鸡的脂肪沉积下降程度有所缓解.  相似文献   

5.
Characterisation of animal genetic resources has been recognised globally as an important step towards their sustainable use. Body weight data of local chickens (213 forest and 160 savannah chickens) and 183 French free-ranging SASSO T44 chickens kept under improved management were collected from hatch to 40 weeks of age and analysed to determine the effects of age, genotype and sex on their growth performance. At all ages, SASSO T44 chickens had significantly (P < 0.05) higher weights (2.6–3.2 kg at 28 weeks) than the local chickens (1.2–1.7 kg at 28 weeks). The rate of growth at the earlier ages in the local genotypes (5.57–7.80 g/day) was lower than the range of 13.81–15.42 in SASSO T44 chickens. Except at hatch, savannah chickens were significantly heavier (P < 0.05) than the forest chickens at all ages. Male chickens had significantly (P < 0.05) superior growth rates than females across all genotypes except from the 20th to the 28th week. Growth trends in both sexes depicted linear increase in body weights; however, the rate of increase in body weights was higher in males as compared to females, thus showing clear sexual dimorphism. There were no significant (P > 0.05) differences in the growth rates of SASSO T44 chickens and local genotypes at the later ages (20–28 weeks). Local chickens from the savannah zone had better growth rate than forest chickens. The significant effect of ecozone on the growth potential of local chickens is an indication that their productive potential could be improved through interventions in the environment such as provision of feed and some veterinary care.  相似文献   

6.
Abstract

Our objective was to examine the effects of bovine growth hormone (bGH) on growth, carcass composition and meat quality of dairy heifers. Nine monozygotic twin pairs of Friesian or Red Danish cattle were used, and pair-fed à diet consisting of grass silage, barley and soybean meal. Within each pair, one animal was given daily subcutaneous injections of 20 IU of pituitary-derived bGH (15–20 mg), while the other animal was injected with saline (excipient). Treatments started at 179±2 kg body weight and lasted for 15.6 weeks. At slaughter, carcass composition and meat quality were analyzed. bGH treatment increased gain by 8% (948 vs. 877 g/d; P <0.03). The amount of saleable meat in carcass and lean content of four main carcass cuts were on average increased by 2% (P <0.05). Concomitantly, carcass fat trim was reduced by 12% (P <0.001), fat trim of individual cuts by 4–21% (P <0.05), and kidney fat by 13% (P <0.01). Except for a 20% reduction of intramuscular fat content (P <0.001), meat quality assessed by objective as well as subjective methods was unaffected by bGH treatment. In summary, bGH treatment of dairy heifers around puberty stimulated growth and reduced carcass fattening including intramuscular fat, while eating quality of the meat was unaffected.  相似文献   

7.
This study was conducted to investigate the effects of free‐range system (FRS) and conventional cage‐rearing system (CRS) on growth performance, carcass yield, meat quality, lymphoid organ indices, and serum biochemistry of Wannan Yellow chickens. At 56 days of age, a total of 640 male chickens were randomly allocated to FRS and CRS groups, each of which included 4 replicates with 80 chickens in each replicate. The experiment lasted from 56 to 112 days of age. The results showed that CRS chickens exhibited better final body weight, average daily feed intake, average daily gain, and feed conversion ratio, whereas FRS chickens showed better breast and leg yields, shear force, meat color, lower drip loss, and decreased abdominal fat deposition. Moreover, the absolute thymus weight and thymus to body weight ratio of FRS birds were significantly higher than those of CRS birds (p < 0.05). Additionally, FRS chickens had significantly reduced glucose, total protein, triglyceride, and cholesterol contents, but enhanced levels of high‐density lipoprotein cholesterol (p < 0.05). In conclusion, the FRS has advantages in breast and leg yields, meat quality, and some serum biochemical parameters of Wannan Yellow chickens, whereas it has negative effects on growth performance.  相似文献   

8.
Distillers dried grains with solubles (DDGS) from corn contain relatively large amounts of polyunsaturated fatty acids and some yeast components, which may increase oxidative stress and alter immune function, respectively, when fed to broilers. Therefore, the study was undertaken to assess the effects of distillers dried grains with soluble (DDGS) on broilers under immunosuppressive challenge. One-day-old male broiler chickens (300) were assigned to 2 treatments with 6 replicates pretreatment. Birds were fed diets formulated to contain 0, 15% corn-based DDGS, respectively. The experimental diets were fed for 6 weeks in 2 phases. On day 21, serum IgA, IgG content and malondialdehyde (MDA), glutathione peroxidase (GSH-Px), total superoxide dismutase (T-SOD), and total antioxidant activity (T-AOC) capacity were analyzed. Chickens were then randomly allotted to 1 of 4 treatment groups: negative control (NC) corn-soybean meal diet without dexamethasone (DEX) challenge, positive control (PC) corn-soybean meal diet with (DEX) challenge, 15% DDGS without DEX challenge (D), 15% DDGS with DEX (D+DEX). Based on these results, dietary DDGS did not influence ADG, ADFI and F:G of 21 d, 28 d and 42 d chicks (P > 0.05), however, DEX affected ADG and F:G of 28 d chicks remarkably (P < 0.05). Relative weights of liver, abdominal fat, spleen, thymus, and bursa were influenced by DEX challenge on d 28 (P < 0.05). DDGS reduced serum T-AOC, T-SOD, whereas increased IgA, IgG and MDA of 21-day-old broilers significantly (P < 0.05). Dietary DDGS also reduced liver T-SOD of 21-day-old broilers significantly (P < 0.05). Based on real-time PCR, 28 d chicks fed DDGS had a greater relative abundance of mRNA encoding IL-4 and IL-6 (P < 0.05), whereas DEX decreased the expression of GPX, IL-6, IL-10 (P < 0.05). Thus, 15% dietary DDGS inclusion has the beneficial effects on immune functions for broilers to some degree.  相似文献   

9.
1. An experiment was carried out to determine the effect of soaking at 0°C, soaking at room temperature, germination, or enzyme treatment of whole barley on feeding value and digestive tract parameters of 2‐ to 4‐week old broiler chickens given diets with 700g/kg whole barley.

2. Soaking or germination decreased the soluble and total β‐glucan content (P<0.05) and, except for soaking at 0°C, the acid extract viscosity of the grain also decreased (P< 0.05). Germination and soaking in the presence of enzymes produced the lowest β‐glucan content and viscosity.

3. Except for soaking in cold water, the soaking, germination and enzyme treatments increased weight gain and decreased food: gain ratio (P< 0.05). Correspondingly, the digestibility of protein, fat, and ash, and the digestible energy content, increased (P< 0.05) after enzyme treatment or germination.

4. Chickens fed on enzyme‐treated or germinated barley diets had intestinal contents with a greater proportion of dry matter and lower viscosity than chickens fed on untreated barley (P< 0.05). Consequently, the cages and chickens were cleaner (P< 0.05) and the weight of digestive organs as proportion of live weight was lower.

5. Particle size analysis of excreta revealed that whole barley was efficiently ground by the gizzards of 16‐d‐old chickens, and very few whole kernels were found.  相似文献   


10.
This study was conducted to assess the effects of dietary corn oil and vitamin E supplementation on fatty acid (FA) profiles and abundances of acetyl‐CoA carboxylase (ACC) and Δ9 stearoyl‐CoA desaturase (SCD) mRNA of Hu sheep. Animals were allocated to three dietary treatments: basal and supplemented with 3% corn oil (CNO), or CNO plus 500 mg/kg vitamin E (COE). The experiment lasted for 10 weeks. No differences were observed in growth performance and carcass qualities among the three treatments (P > 0.05). Feeding CNO and COE diets increased polyunsaturated FAs including cis 9 trans 11 conjugated linoleic acid, and decreased saturated FA in longissimus muscle (P < 0.05). The mRNA abundances of ACC and SCD as detected by real‐time PCR were reduced (P < 0.05) in liver and subcutaneous fat by supplementary oil, while the SCD mRNA level in longissimus muscle was also reduced (P < 0.05). Inclusion of vitamin E did not have further effects on mRNA abundances of these two enzymes. It is suggested that dietary corn oil supplementation may reduce FA biosynthesis and influence FA profiles in Hu sheep through decreased expression of both ACC and SCD genes.  相似文献   

11.
Krüppel‐like factor 7 (KLF7) has been extensively studied in mammalian species, but its role in birds is still unclear. In the current study, cloning and sequencing showed that the full‐length coding region of chicken KLF7 (Gallus gallus KLF7, gKLF7) was 891 bp long, encoding 296 amino acids. In addition, real‐time RT‐PCR analysis showed that gKLF7 was broadly expressed in all 15 chicken tissues selected, and its expression was significantly different in spleen, proventriculus, abdominal fat, brain, leg muscle, gizzard and heart between fat and lean broilers at 7 weeks of age. Additionally, one novel single nucleotide polymorphism (SNP), XM_426569.3: c. A141G, was identified in the second exon of gKLF7. Association analysis showed that this locus was significantly associated with fatness traits in Arbor Acres broiler random population and the eighth generation of Northeast Agricultural University broiler lines divergently selected for abdominal fat content (NEAUHLF) population (P < 0.05). These results suggest that gKLF7 might be a candidate gene for chicken fatness traits.  相似文献   

12.
ABSTRACT This experiment was conducted to investigate leptin mRNA expression, adipocyte size, and their relationship in several adipose tissues of fattening steers. Subcutaneous, perirenal, intermuscular and intramuscular adipose tissues were collected from three crossbred steers (Japanese Black cattle X Holstein) aged 21 months. The mRNA level and adipocyte diameter were determined in these adipose tissues. The intramuscular adipose tissue had a lower leptin mRNA level than the intermuscular and perirenal adipose tissues (P < 0.05). Leptin mRNA level was lower in the subcutaneous depot than in the intermuscular depot (P < 0.05). Adipocyte diameter was larger in the intermuscular adipose tissue than in the subcutaneous and intramuscular adipose tissues (P < 0.05). Leptin mRNA level was positively correlated with adipocyte diameter (r2 = 0.81, P < 0.05). These results suggest that the cattle have fat depot‐specific differences in leptin gene expression, which are a result of a difference in adipocyte size.  相似文献   

13.
The principal objective of this study was to assess whether bedding types could influence the growth performance of broiler chickens fed on high and low nutrient density diets. The broilers were bedded with clean hulls or shavings. Four hundred eighty male broiler chickens (Ross) were divided into 6 treatment groups, including 1) a high-density diet with rice hulls, 2) a high-density diet with wood shavings, 3) a high-density diet with coconut hulls, 4) a low-density diet with rice hulls, 5) a low-density diet with wood shavings, and 6) a low-density diet with coconut hulls. Weight gain, feed intake, G:F, visceral organ relative weight, red blood cells, white blood cells, and lymphocyte concentrations were evaluated. The bursa of Fabricius, liver, and abdominal fat relative weight, white blood cells, and lymphocyte concentrations were not altered consistently by any of the treatments. For the overall period, weight gain and feed intake were greater in the coconut hull treatment groups as compared with the wood shavings treatments (P < 0.05). Feed intake was also greater in the low-density diet than in the high-density diet treatment groups (P < 0.05). The concentration of red blood cells in blood was reduced significantly by wood shavings bedding compared with the rice hull treatments (P < 0.05).  相似文献   

14.
We reported previously that behavioral development of chicks was promoted remarkably by the presence of a broody hen. Here we report that these effects at an early age persist after maturity. A total of 60 female chicks were randomly assigned to one of two treatment groups: six pens with five chicks (brooded group) each were reared by a broody hen and six pens with five chicks (non‐brooded group) each were provided with an infrared heating lamp. We evaluated the persistent effects of broody hens by measures of behavior, physical condition and production at 9, 16, 35 and 55 weeks of age. The numbers of threatening, aggressive pecking, fighting and severe feather pecking behaviors were higher in non‐brooded than in brooded chickens (all P < 0.05). Egg production was lower in brooded than in non‐brooded chickens (P < 0.05), while the number of brooding chickens was higher in the brooded than in the non‐brooded group (P < 0.05). In conclusion, the presence of broody hens at an early stage of chicks' lives has a persistent effect on behavior. Although brooded chickens showed more brooding and lower egg production than non‐brooded chickens, feather pecking and aggressive interaction were decreased in brooded hens.  相似文献   

15.
Kapok seed and oil from the tropical zone are widely used as pig feed to harden porcine fat in Japan. This study evaluated the effect of dietary kapok oil supplementation on pork quality and sensory traits. Five Duroc pigs each were assigned to an experimental group supplemented with kapok oil and a control group. Dietary kapok oil supplementation had no effect on growth performance and intramuscular fat content in the Longissimus dorsi muscle (LM). Supplemental kapok oil increased saturated fatty acid contents in subcutaneous and intramuscular fat and decreased monounsaturated fatty acid levels (P < 0.05). Off‐flavor detection by a trained panel was higher in the experimental than the control group (P < 0.05), but tenderness, juiciness, texture and flavor intensity of LM chops were similar in both groups. The overall palatability of pork as judged by a consumer panel decreased with kapok oil supplementation (P < 0.01). These results indicate that while growth performance, intramuscular fat contents and carcass characteristics were unchanged, while dietary kapok oil supplementation makes firm fat to prevent inferior soft fat in pork, it can lower the palatability of pork due to a decrease in monounsaturated fatty acids.  相似文献   

16.
ABSTRACT

1. Lipid metabolism is an indispensable process in an organism, though little is known about the regulatory mechanisms of fat deposition in different types of adipose tissues.

2. The differentially expressed genes related to triglyceride (TG) metabolism between abdominal and intramuscular fat (IMF) of Beijing-You chickens were investigated in this study.

3. TG content in abdominal fat (AF) (349.7 mg/g) was significantly higher (P < 0.01) than in the breast and thigh (12.3 mg/g and 24.8 mg/g, respectively).

4. Using Agilent chicken gene-expression profiling in adipose tissues between AF and muscle (breast and thigh), certain representative genes related to fatty acid metabolism, lipoprotein catabolism and esterification reactions were significantly upregulated (P < 0.05 or P < 0.01).

5. Genes involved in fatty acid oxidation or carbohydrate utilisation were significantly up- or downregulated (P < 0.05 or P < 0.01), including those involved with highly enriched pathways of lipid metabolism (PPAR, Wnt pathway and inositol phosphate metabolism), cell junctions (focal adhesion and regulation of actin cytoskeleton) and muscle contraction.

6. Overall, higher TG levels were observed in AF tissue than in adipose tissues of breast and thigh, which could be regulated through gene expression of pathways related to lipid metabolism (PPAR, Wnt pathway and inositol phosphate metabolism), cell junctions (focal adhesion and regulation of actin cytoskeleton) and muscle contraction. These results provide clues to understanding the molecular mechanisms of TG metabolism between abdominal and IMF.  相似文献   

17.
Twenty 10‐month‐old Japanese Black steers were used to evaluate the effects of clay on fat necrosis and carcass characteristics. Ten steers (Clay group) were fed the clay (50 g/day) during 10–30 months of age. The other 10 steers (Control group) were not fed it. There was no significant difference in body weight or average daily gain between the two groups (P > 0.05). The occurrence of fat necrotic mass in the Clay group (30%) was lower (P < 0.05) than that in the Control group (90%) at slaughter. The size of necrotic masses in the Clay group was smaller (P < 0.05) than that in the Control group. There was no significant difference in the marbling score, beef color, Longissimus muscle area or subcutaneous fat thickness between the two groups. These results suggest that the clay prevented the occurrence of fat necrosis and did not affect the carcass characteristics in Japanese Black steers.  相似文献   

18.
The present study assessed the effect of feeding palm oil (PO), sunflower oil (SO) and their combination on performance, fat deposition, fatty acid composition and lipogenic gene expression of broilers reared for 42 days. A total of 144 1‐day‐old broilers (Cobb500) were randomly allotted into four treatment diets with each having six replicates of six chicks in each replicate following a completely randomized design. Live weight gain and feed efficiency was significantly (P < 0.05) higher in birds fed with a combination of oil sources compared to controls. Birds fed with the combination of oil and SO alone had higher carcass yield and lower abdominal fat. Higher (P < 0.05) concentrations of unsaturated fatty acids (UFA) and lower concentrations of palmitic acid and saturated fatty acid (SFA) was found in birds fed SO alone and combinations of SO and PO. Furthermore, the outcomes showed that birds fed diet supplemented with SO and the combination of SO and PO down‐regulated gene expression of key hepatic lipogenic enzymes of fatty acids synthase (FAS), acetyl‐CoA carboxylase (ACC) and stearoyl‐CoA desaturase (SCD). These findings suggest that the diet containing the combination of 2% PO and 4% SO may reduce hepatic lipogenesis, as well as lower abdominal fat content of broilers.  相似文献   

19.
为揭示脂肪分化相关蛋白(adipose differentiation-related protein,ADFP)变化对家禽脂肪性状形成的作用,实验采用SYBRGreenΙ荧光定量法,首次测定了四川山地乌骨鸡1、14、28、42、56、70d和84d胸肌、肝脏、腹脂和皮脂组织中ADFP基因相对表达量。结果表明:ADFP基因表达量在28d,腹脂和肝脏中的表达量显著高于胸肌(P<0.05),且腹脂>肝脏>皮脂>胸肌;在42d,胸肌中的表达量显著高于皮脂(P<0.05),且胸肌>肝脏>腹脂>皮脂;在84d时,胸肌中的表达量显著高于肝脏中的(P<0.05),且胸肌>腹脂>肝脏。腹脂中ADFP基因的相对表达量的变化与活重和冠重性状的变化显著相关(P<0.05),胸肌中ADFP基因的相对表达量发育性的变化与肌内脂肪含量的变化显著相关(P<0.05)。ADFP基因可能在鸡脂肪性状形成的过程中发挥重要的调控作用。  相似文献   

20.
Body composition and energy requirements are different between males and females in several species, and both interfere with weight loss. The aim of this study was to compare the total and regional body composition and energy requirements in obese male (n = 8) and female (n = 8) cats, during weight loss and weight maintenance over 17 subsequent weeks after regimen. The total and regional (thoracic and pelvic limbs, and trunk) body composition was assessed by dual‐energy X‐ray absorptiometry (DXA). Females exhibited a higher fat mass (FM) than males (p < 0.05), and the trunk was the site with greater fat accumulation regardless of gender. A 23.0 ± 2.8% reduction in body weight was followed by a 50.3 ± 9.4% and a 37.0 ± 8.9% reduction in fat in the trunk region in males and females respectively. Lean mass (LM) mobilization was also increased in the trunk (p < 0.05), and the loss of LM was associated with a reduction in bone mass. The energy intake to achieve the same rate of weight loss was 12.9 ± 3.4% higher in males (p < 0.05). The cats exhibited a gradual increase in energy requirements to maintain their body weight after weight loss (p < 0.05). It was concluded that obese cats mainly accumulate fat in the trunk. The reduction in lean mass after the regimen also occurred in the trunk, with no modifications in the skeletal muscle mass of the limbs. Neutered male cats have higher energy requirements than neutered females, and gender should be considered during obesity management in cats.  相似文献   

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