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家兔豆状囊尾蚴病暴发感染调查 总被引：1，自引：1，他引：0
家兔豆状囊尾蚴病是由豆状带绦虫 ( Taeniapisiformis)的幼虫——豆状囊尾蚴寄生于肝脏、肠系膜、网膜及骨盆腔而引起。寄生数量少时 ,可无明显临床症状 ,大量感染则可引起肝脏损害、消化功能紊乱、消瘦倦怠 ,严重者可引起死亡。1 995年 5月 ,我中心饲育的家兔虽经常规疫苗注射、球虫病及螨病防治等 ,仍普遍表现出不同程度的精神沉郁、被毛松乱、消瘦、腹泻患病率高及整体生长发育迟缓等 ,影响了正常的生产。抽样剖检部分术后兔及死亡兔 ,发现有豆状囊尾蚴感染 ,于是就对兔群进行了较系统的豆状囊尾蚴感染情况调查 ,并采取了相应防治措施 ,… 相似文献
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家兔豆状囊尾蚴病是由豆状带绦虫的幼虫(即豆状囊尾蚴)寄生于家兔的肝脏、肠系膜和腹腔内而引起的一种寄生虫病。2012年滨州畜牧兽医研究院门诊部共接收2例囊尾蚴病例,发病多与场内饲养猫或犬有关。兔场内老鼠多,很多场内饲养了猫来防治老鼠,殊不知猫传染疾病的危险性也非常大,兔囊尾蚴病就是与犬、猫等肉食 相似文献
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1 兔豆状囊尾蚴病兔豆状囊尾蚴病是由豆状带绦虫的幼虫寄生于家兔体内引起的一种寄生虫病。本病的流行需要犬等肉食动物作为终末宿主,一般在养犬的养殖场较为多发。1.1 流行病学 兔豆状囊尾蚴病呈世界性分布,在我国家兔主产区都有不同程度的感染。犬、猫等肉食动物是本病的终末宿主,而兔是本病的中间宿主。主要传播方式为消化道,感染成虫的犬、猫通过粪便排出虫卵孕节或虫卵污染饲料、饲草、饮水等,家兔通过食入这些被污染的饲草、饮水而感染发病。 相似文献
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Qiuxia Wang Shaohua ZhangXuenong Luo Junling Hou Xueliang ZhuXuepeng Cai 《Veterinary parasitology》2013
Rabbit cysticercosis, caused by the larval stage of Taenia pisiformis, is a serious parasitic disease of rabbits. It was reported that some cysteine peptidases have potential roles in the pathogenesis of various parasitic infections. To investigate the biochemical characteristics and roles in the pathogenesis/host-invasion of cysteine peptidases, a cDNA sequence encoding for a cathepsin L-like cysteine protease (TpCP) was cloned and identified from the T. pisiformis metacestodes. This sequence was 1220 bp in its length, which included a 1017 bp open reading frame encoding a 339 amino acid peptide. Multiple sequence alignments revealed a 28.9–88.5% similarity with cathepsin L-like cysteine proteases from other helminth parasites and mammals. The recombinant TpCP expressed in Escherichia coli did not show the proteolytic activity by zymography gel assay. However, the TpCP expressed in Pichia pastoris had typical biochemical activities that could hydrolyze rabbit immunoglobulin G, bovine serum albumin and fibronectin. Substrate studies indicated pronounced cleavage of Z-Phe-Arg-AMC. This activity was sensitive to cysteine protease inhibitor E-64 and immunohistochemistry results also indicated that TpCP was distributed as an intense positive reaction in the bladder wall. Our results gave us insights into future studies of TpCP's roles in the infection. 相似文献
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Kebede N 《Research in veterinary science》2008,85(3):522-526
The occurrence of cysticercosis due to Taenia saginata in cattle slaughtered for meat in Amhara National Regional State, northwestern Ethiopia between September 2005 and February 2007 was investigated. Routine meat inspection of various organs of 4456 cattle in eight abattoirs of this region showed that 824 (18.49%) were infected with Cysticercus bovis. The occurrence rate did not vary significantly from abattoir to abattoir (P>0.5). The tongue, masseter muscles, heart muscles, triceps muscles and thigh muscles were the main predilection sites of the cysts. Of 4102 male cattle, examined, 768 (18.72%) had cysts of C. bovis while 56 (15.82%) of the 354 female animals investigated were infected. The animals slaughtered were all adults. No significant difference in occurrence was recorded between the sexes. Monthly occurrence of the cysts in the animals revealed a rise of infected animals during the dry season. 相似文献
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The prevalence of Taenia saginata cysticercosis in cattle slaughtered for meat in Addis Ababa Abattoir, Ethiopia between September 2004 and August 2005 was
reported. The examination of various organs of 11227 cattle in Addis Ababa Abattoir showed that 842 (7.5%) were infected with
T. saginata cysticercosis. The tongue, masseter muscles, cardiac muscles, triceps muscles and thigh muscles were the main predilection
sites of the cysts. The cysts of bovine cysticercosis were also identified on the spleen, intercostal muscles, diaphragm and
liver. Out of 10329 male cattle, examined, 783 (7.6%) had cysts of bovine cysticercosis while 59 (6.6%) of the 898 female
animals investigated were infected. The animals slaughtered were all adults. No significant difference in prevalence rates
was recorded between the sexes. The prevalence of bovine cysticercosis was higher in local zebu cattle breeds than Holstein-Frisian
cattle. 相似文献
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Maio E Carta T Balseiro A Sevilla IA Romano A Ortiz JA Vieira-Pinto M Garrido JM de la Lastra JM Gortázar C 《Research in veterinary science》2011,91(2):212-218
Of the non-ruminant wildlife species known to harbor Mycobacterium avium paratuberculosis (MAP), the rabbit (Oryctolagus cuniculus) is thought to pose the greatest risk of transmission to cattle. We analyzed 80 hunter-harvested wild rabbits from a core study area in southern Spain, and sera from 157 wild rabbits sampled opportunistically on seven additional sites. Gross lesions compatible with paratuberculosis were observed in two of 80 necropsied rabbits. Histopathology revealed focal to diffuse multibacillary MAP-compatible lesions in 8 of 10 rabbits examined. Presence of MAP was confirmed in one rabbit with gross lesions by positive amplification curves for both IS900 and ISMAP02. However, no isolate was obtained from 47 samples by culture. We adapted an indirect ELISA for the detection of MAP antibodies. At the established cut-off of 0.5, 6 of 237 wild rabbit sera (2.5%) yielded a positive ELISA result. Antibodies were detected in rabbits from 3 of 8 sampling sites. Considering the increasing relevance of MAP infection for animal health, these results open a challenging field for future research. 相似文献
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Andr M. Almeida Alexandre Campos Sofia van Harten Luís Alfaro Cardoso Ana Varela Coelho 《Research in veterinary science》2009,87(2):196-199
In several laboratory and production species, the establishment of a proteome reference map of a specific tissue has been accomplished. The rabbit is widely used as both a production and experimental animal. A lot of physiology research involving the gastrocnemius muscle of rabbit is described, although no reference proteome map is available. In this work, the first reference map of the rabbit’s gastrocnemius muscle using 2D gel electrophoresis and the identification of proteins through peptide mass fingerprinting (PMF) was established. A total of 45 proteins were localized and identified with three major roles: cell structure and contractile apparatus; metabolic and cell defense proteins. A reference map of major proteins expressed is described enabling possible comparisons with other physiological studies. 相似文献
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Herbert S. Soares Sílvia M.M. Ahid Ana C.D.S. Bezerra Hilda F.J. Pena Ricardo A. Dias Solange M. Gennari 《Veterinary parasitology》2009,160(3-4):211-214
Toxoplasma gondii is an obligate intracellular parasite with a variety of hosts, responsible for reproductive problems and economic losses in sheep flocks. Neospora caninum was recently identified and its clinical presentation in sheep is similar to that of toxoplasmosis, which can cause repeated abortions, though less frequently in this species. In order to confirm the prevalence of these agents in the city of Mossoró, Rio Grande do Norte, Brazil, 409 serum samples from adult sheep (364 females and 45 males) were tested by the indirect immunofluorescence antibody test, using cut-off point at a dilution of 1:64 and 1:50 for T. gondii and N. caninum, respectively. From the 35 properties examined, 23 (65.7%) had at least one seropositive animal for T. gondii and six (17.1%) for N. caninum. The prevalence of seropositive animals for T. gondii was 20.7% and for N. caninum 1.8%. There was no association between the presence of the agent’s antibody and gender, reports of reproductive problems and presence of dogs and/or cats in the properties. T. gondii is well distributed and N. caninum has low prevalence in sheep and in the properties of the studied region. 相似文献
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Danny Morick Gad Baneth Boaz Avidor Michael Y. Kosoy Kosta Y. Mumcuoglu Dvir Mintz Osnat Eyal Ralph Goethe Andreas Mietze Nahum Shpigel Shimon Harrus 《Veterinary microbiology》2009,139(3-4):293-297
The prevalence of Bartonella spp. in wild rodents was studied in 19 geographical locations in Israel. One hundred and twelve rodents belonging to five species (Mus musculus, Rattus rattus, Microtus socialis, Acomys cahirinus and Apodemus sylvaticus) were included in the survey. In addition, 156 ectoparasites were collected from the rodents. Spleen sample from each rodent and the ectoparasites were examined for the presence of Bartonella DNA using high resolution melt (HRM) real-time PCR. The method was designed for the simultaneous detection and differentiation of eight Bartonella spp. according to the nucleotide variation in each of two gene fragments (rpoB and gltA) and the 16S–23S intergenic spacer (ITS) locus, using the same PCR protocol which allowed the simultaneous amplification of the three different loci. Bartonella DNA was detected in spleen samples of 19 out of 79 (24%) black rats (R. rattus) and in 1 of 4 (25%) Cairo spiny mice (A. cahirinus). In addition, 15 of 34 (44%) flea pools harbored Bartonella DNA. Only rat flea (Xenopsyla cheopis) pools collected from black rats (R. rattus) were positive for Bartonella DNA. The Bartonella sp. detected in spleen samples from black rats (R. rattus) was closely related to both B. tribocorum and B. elizabethae. The species detected in the Cairo spiny mouse (A. cahirinus) spleen sample was closely related to the zoonotic pathogen, B. elizabethae. These results indicate that Bartonella species are highly prevalent in suburban rodent populations and their ectoparasites in Israel. Further investigation of the prevalence and zoonotic potential of the Bartonella species detected in the black rats and the Cairo spiny mouse is warranted. 相似文献
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Hsu YM Lin CC Chomel BB Tsai KH Wu WJ Huang CG Chang CC 《Comparative immunology, microbiology and infectious diseases》2011,34(6):513-518
Rickettsia spp. are zoonotic pathogens and mainly transmitted by various arthropod vectors, such as fleas, ticks, and lice. Previous epidemiological studies indicated that ectoparasites infested on dogs or cats may be infected by Rickettsia spp., and transmit them to human beings accidentally. In this study, the prevalence of Rickettsia infection was evaluated using fleas and ticks from stray dogs and cats in Taiwan. A total of 158 pools made by 451 cat fleas (Ctenocephalides felis) from 37 dogs and 4 cats were used for analysis. Besides, 386 Rhipicephalus ticks collected from the other 62 stray dogs were included in this study. Nymphal and adult ticks were individually analyzed but larvae were separated into 21 pools for molecular detection. Partial sequencing analysis of the gltA gene was applied for Rickettsia identification. The results showed that 44.3% (70/158) of the cat flea pools were harboring Rickettsia DNA. Although 6.9% (13/187) of adult ticks were infected with Rickettsia, neither larval pools nor nymphal ticks were found to contain Rickettsia DNA. According to the results of sequencing analyses, all Rickettsia PCR-positive cat flea pools were infected with R. felis, and all Rickettsia PCR-positive adult ticks were infected with R. rhipicephali. The results of this study demonstrated that C. felis but not Rhipicephlus sanguineus (the brown dog tick) and Rh. haemaphysaloides collected from stray animals in Taiwan could be infected the zoonotic pathogen R. felis. Moreover, R. rhipicephali was only identified in adult stage of Rhipicephalus sanguineus and Rh. haemaphysaloides. 相似文献
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应用PCR技术对3个猪囊尾蚴西昌分离株的线粒体细胞色素C氧化酶第Ⅰ亚基(cox1)基因全序列和烟酰胺腺嘌呤二核苷酸还原酶第一亚基(nad1)基因部分序列进行扩增,分析其遗传变异,并用Mega 5.0程序NJ法绘制种系发育树,探讨不同地区来源的猪囊尾蚴种系发育关系。测序结果显示,猪囊尾蚴的cox1基因全序列长度为1 620bp,nad1基因部分序列长度为796bp。cox1和nad1基因序列均存在一定的遗传变异,且cox1基因序列的变异高于nad1序列。种系发育分析结果显示,所有猪囊尾蚴分离株构成一个分支,3个西昌分离株均属于猪带绦虫亚洲基因型。结果表明,cox1和nad1基因均可用于猪带绦虫的分子分类,为猪带绦虫病/猪囊尾蚴病的分子诊断奠定了基础。 相似文献
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Lucas L. Asaava Phillip M. Kitala Peter B. Gathura Mark O. Nanyingi Gerald Muchemi Esther Schelling 《Preventive veterinary medicine》2009,89(3-4):197-204
Bovine cysticercosis is a zoonosis that is mainly of socioeconomic and public health importance. A survey of this disease was carried out in Northern Turkana District, Kenya to estimate the prevalence through both serology and meat inspection, to determine the prevalence of the adult tapeworm in the human definitive host, and to determine risk factors for cattle seropositivity. This information is of public health importance and will be of use in assessing economic losses due to downgrading, refrigeration or condemnation of infested carcasses.The study area was stratified into the three livestock grazing regions of Oropoi to the south, Lokichoggio–Mogilla centrally and Kibish in the north for the purposes of the serological and questionnaire (n = 53 herd owners) data. Five adakaars (grazing units) were selected and 34, 63, 49, 75 and 571 cattle serum samples obtained from these. The slaughter slabs of Lokichoggio and Kakuma were visited and 188 serum samples were obtained from slaughter cattle and compared to results of meat inspection. Human stool samples were collected in each of the three grazing areas and 66, 97 and 78 samples were obtained.The seroprevalence of cysticercosis in cattle was estimated at 16.7% (95% CI 13–20.9%) using a secretory–excretory antigen detection ELISA. There was poor agreement between meat inspection and serology (k = 0.025; p = 0.2797). The prevalence of taeniosis was estimated as 2.5% (95% CI 0.8–5.6%) by microscopy.A backwards elimination logistic regression analysis indicated that the grazing unit (Adakaar), the deworming history of household members and the distance (>2 km) of grazing fields from the homestead were significant explanatory variables for cattle being found to be positive on serology. An intra-cluster correlation coefficient (ICC) of 0.07 (0.02–0.12); p < 0.0001 was calculated for bovine cysticercosis in this area. 相似文献