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1.
A small tumour was found at the periphery of the lung during routine necropsy of a 13-year-old Romney ewe. The tumour consisted of vacuolated, cuboidal cells which lined alveolar spaces without destroying them. Electron microscopy revealed numerous microvilli on the free surface of the cells and irregularly shaped osmiophilic inclusions within the vacuoles. No viral particles were found and the tumour was diagnosed as a well differentiated Type II pneumocyte adenocarcinoma of unknown aetiology.  相似文献   

2.
A case of a canine large cell type T-cell lymphoma, with features of high-grade malignancy is described. The tumour was found confined in the nasal cavity and the paranasal sinuses of a crossbred German Shepherd dog. Histological examination revealed the features of a highly malignant large cell lymphoma. Ultrastructurally, the lymphoid tumour cells bore cytoplasmic protrusions that interdigitated tightly. From a panel of tumour markers used, the neoplastic cells were stained only for vimentin. Immunophenotyping of the tumour cells by means of CD3, CD79, kappa-light chains and lambda-light chains detection was undertaken. The tumour stained only for CD3 and was classified as T-cell lymphoma.  相似文献   

3.
A cerebral tumour was found in the right frontal lobe of a 7-year-old female mongrel cat. The mass showed infiltrative growth and caused deformation of the corpus callosum. Histopathologically, the tumour cells showed anaplasia, pleomorphism and mitotic figures. Necrosis and vascular proliferation were prominent. The neoplastic cells surrounded areas of necrosis, but as an indistinct pseudopalisade formation. Immunohistochemically, low numbers of tumour cells labelled positively for anti-glial fibrillary acidic protein and anti-S100 protein. Electron microscopically, the majority of tumour cells had no filaments and cytoplasmic processes, but the differentiated cells presented cytoplasmic filaments and glycogen granules. Based on these findings, the tumour was diagnosed as cerebral high-grade astrocytoma, glioblastoma.  相似文献   

4.
The morphology and morphogenesis of jaagsiekte retrovirus (JSRV)   总被引：1，自引：0，他引：1  
Jaagsiekte retrovirus ( JSRV ) was recently shown to be the aetiological agent of jaasiekte (ovine pulmonary adenomatosis). The morphogenesis of JSRV was studied in jaagsiekte tumour tissue. Intracytoplasmic particles, often associated with centrioles, were found in tumour cells. JSRV budded from tumour cells with a complete core which appeared to mature during the budding process. Extracellular particles were found in the alveolar lumen. Immature extracellular particles were rare. Mature extracellular JSRV was membrane-bound and had a slightly eccentric nucleoid with an electron-dense perinucleoidal space. In negatively stained preparations of JSRV the envelope was covered with spikes. JSRV is morphologically distinct from all known retroviruses.  相似文献   

5.
In this study, a case of osteoclast-like giant cell tumour arising from the kidney is reported in an eight-year-old female Anatolian Shepherd dog. Macroscopically, the tumorous mass covered the hilus of the left kidney. It was 26 x 22 x 12 cm in size and 3700 g in weight. Metastatic tumorous nodules, 0.5-2.0 cm in diameter, were found on the abdominal side of the diaphragm and in the lungs. Microscopically, numerous large osteoclast-like multinucleated giant cells and spindle-spheroidal-shaped cells were seen. Osteoblastic differentiation and osteoid matrix were noted in a few areas at the periphery of the tumour, near the connective tissue septa. The stroma of the tumour tissue was vascular, oedematous and loose. By immunoperoxidase staining, tumour cells showed immunoreactivity for vimentin but not for keratin and desmin, indicating that the tumour had mesenchymal origin. This is the first report in the literature on a malignant osteoclast-like giant cell tumour arising from a visceral organ in animals.  相似文献   

6.
The canine transmissible venereal tumour (CTVT) is a transmissible cancer that is spread naturally between dogs, with the ability to develop and evade the immune system, despite strict immune surveillance of the host. Furthermore, molecular signalling between cells of the immune system and the tumour microenvironment appear to influence the behaviour and development of the tumour. Thus, this study aimed to quantify the expression of genes related to the immune system such as IL‐6, IFN‐γ, and TGF‐β, as well as angiogenic factors (VEGF, CXCR4), in CTVT cells in vivo and in vitro (primary culture), correlating with the clinical response of the animals treated with vincristine. As expected, the most prevalent subtype was plasmacytoid cells, although lymphocytic cells were also found, indicating the possibility of polyclonality. When we compared the gene expressions of IFN‐γ and IL‐6, we mostly found low expression, concluding that MHC expression was probably not occurring in tumour cells, and no activation of immune cells to eliminate the tumour. The TGF‐β gene was normal in the majority of animals but demonstrated decreased expression in vincristine resistant animals, leading to the hypothesis that the concentration of tumour‐derived TGF‐β was affecting and even suppressing the real TGF‐β expression, favouring tumour proliferation and progression in these cases. VEGF expression was extremely high, demonstrating its angiogenic role in tumour growth, while CXCR4 was decreased, possibly because of CTVT’s low metastatic potential. Thus, we concluded that the tumour microenvironment, together with the immune system of the host, influences CTVT, presumably altering its tumorigenesis and the animal’s clinical response to treatment.  相似文献   

7.
Clinicopathological and immunohistochemical findings of two uncommon canine lipid-rich mammary carcinomas are described. The predominant histological feature in both tumours was the presence of at least 80% of cells with intracytoplasmic vacuoles which stained positively with Sudan IV but not with alcian-blue periodic acid-schiff method. In both tumours, small groups of non-vacuolated cells were identified among the vacuolated cells. However, histological and immunohistochemical differences were also found between these tumours. Thus, one of them was composed of tumour cells with a large and single vacuole, which were arranged in lobular pattern, while the other neoplasm showed an intraductal growth of tumour cells with a fine vacuolated cytoplasm. Immunohistochemically, in the first tumour most vacuolated cells were positive for CK (cytokeratin)8-7, indicating a secretory epithelial immunophenotype while CK5 and CK8-7-expressing non-vacuolated cells were associated with luminal duct immunophenotype. However, in the second tumour the expression of CK14 in most of vacuolated cells and alpha-smooth muscle actin (alpha-SMA) in non-vacuolated cells, alone or in combination with CK5 suggested a myoepithelial immunophenotype for both cell types. These results suggest heterogeneity of the cell type and growth pattern for this type of canine tumour as has been described in women but not in dogs.  相似文献   

8.
Introduction : Canine transmissible venereal tumour is occasionally observed in leishmaniotic dogs, and Leishmania amastigotes can be harboured in canine transmissible venereal tumour cells. Objectives : The aim of this paper was to investigate the clinicopathological significance of the association of both diseases. Methods : Nineteen dogs affected by canine transmissible venereal tumour and canine leishmaniasis were studied retrospectively. Results : In these dogs, the tumour manifested a large size and often aggressive behaviour (42%) and no predictive sign of spontaneous regression was observed. Sporadic Leishmania amastigotes were found within the canine transmissible venereal tumour in three cases, probably transported by infected macrophages often infiltrating the tumour. A high Leishmania parasitisation of canine transmissible venereal tumour was observed in two other cases and verified by immunohistochemistry. Clinical Significance : Canine transmissible venereal tumour is a tumour of the dog able to harbour a large number of Leishmania parasites. Alternatively, the systemic disease (canine leishmaniasis) may lower the immune defence against malignancy (canine transmissible venereal tumour).  相似文献   

9.
An 8-year-old castrated Cocker Spaniel with tympanic paraganglioma was presented. The tumour was resected twice with an interval of 120 days. The dog presented with tetraparesis 130 days after the second operation and had radiologic evidence of metastasis to the C5 vertebra. On pathologic examination, metastasis of the tumour was found in the myocardium, lungs, kidney, and C5 vertebra. The tumour, which is being described for the first time in the dog, was highly vascular, and was characterized by various-sized groups of medium-sized cells separated by vascular stroma. The neoplastic cells were round or polyhedral with moderately hyperchromatic nuclei, scattered chromatin, indistinct nucleoli, and eosinophilic granular cytoplasm. The metastatic lesions were histologically similar to the primary tumour.  相似文献   

10.
Vascular changes at various stages of growth of 7,12‐dimethylbenz(α)anthracene (DMBA)‐induced mammary adenocarcinomas in 22 female Sprague‐Dawley rats were investigated using histology, immunohistochemistry and scanning electron microscopy (SEM) of corrosion casts. In the early stage of tumour growth, capillaries within the neoplasms were thin, with 8–10 μm diameter, and characterized by rows of vascular sprouts representing extensive neovascularization and formation of a high‐density capillary plexus. In the intermediate stage of tumour growth, the growing tumour was multi‐nodular and tumour cells were arranged in a tubulopapillary pattern. Capillaries formed spheroid vascular capsules and were characterized by dilation, to a diameter of 10–80 μm, and blind ends. In the late stage of tumour growth, a remarkable reduction in the number of vascular endothelial growth factor‐positive cells and Ki‐67‐stained nuclei was demonstrated. The tunica media of nutritive arteries displayed a tendency to atrophy. Many arteriovenous anastomoses between the major arteries and the veins were found in regions just before their entry into the tumour. The central regions of the tumour were degenerative and necrotic, and vasculature was confined to surface regions of the tumour, forming a basket‐like configuration around the avascular central regions. Resin leakages representing haemorrhage or oedema and distortions such as flattening, break‐off and strangulations of capillaries were frequently observed, all of which are known as late tumour signs. We concluded that these microvascular alterations might change the homogeneity of tumour perfusion and contribute to necrosis in central portions of the tumour.  相似文献   

11.
A solitary mass, 2.5 cm × 2.5 cm × 2.0 cm in size, was found in the subcutis adjacent to the ischial tuberosity of an 8-year-old male German shepherd dog. The excised mass was not encapsulated and the cut surface was solid, grey–white and had multiple red areas suggestive of haemorrhage. Microscopically, the neoplasm was composed of epithelioid cells interspersed with venous vessels of various sizes and irregular bundles of spindle-shaped tumour cells with mucinous stroma. The nuclei showed anisokaryosis and many mitotic figures were noted. Immunohistochemically, the majority of tumour cells were positive for alpha smooth-muscle actin and vimentin, but negative for cytokeratin, desmin, S-100 and factor VIII-related antigen. Ultrastructurally, the tumour cells contained irregular nuclei, few mitochondria, few rough endoplasmic reticulum, cytoplasmic myofilaments of intermediate density, pinocytotic vesicles and distinct basal lamina. Based on these pathological findings, the diagnosis was malignant glomus tumour.  相似文献   

12.
A soluble tumour-associated antigen was prepared by homogenizing AKR lymphoma cells (L15) followed by treatment with acetone and glycine-HCl buffer pH 3. It was analyzed by immunoelectrophoresis, rocket electrophoresis in the presence of Concanavalin A and SDS-PAGE. It showed rapid electrophoretic mobility, and the major component had an estimated molecular weight of 70,000 daltons. The molecular composition of the antigen included a glycoprotein. Specific antibodies against this antigen were demonstrated in BALB/c mice in which the AKR lymphoma was conditioned to grow by introducing the L15 cells into a subcutaneously implanted glass cylinder. In this model antibodies were found in both tumour-bearing (progressor) and tumour-rejecting (regressor) animals. In vivo experiments showed that pretreatment of BALB/c mice with L15 acellular extracts, 10 days before tumour challenge, led to a significant increase in allogeneic tumour growth. A rabbit anti-tumour extract serum was prepared and was rendered tumour -specific by exhaustive absorption with normal AKR serum and tissues. It shared the same epitopic specificity with tumour progressor or regressor mouse sera since indirect immunofluorescence using L15 cells could be reciprocally inhibited.  相似文献   

13.
利用ELISA试剂盒,对百日鸡的禽白血病抗体和抗原进行了检测。在5个百日鸡父母代鸡群中,1个鸡群的ALV-J抗体阳性率为57.14%,其余鸡群皆为阴性;所有鸡群的ALV-A、B抗体均为阴性;ALV p27抗原除1个鸡群为阴性外,其余鸡群阳性率都较高。在百日鸡的J亚群禽白血病病例中,肝脏、脾脏和肾脏显著肿大,且有弥漫性分布的肿瘤结节;在其他脏器也呈现肿瘤结节。组织病理学观察,发现其脏器等组织中,有一定比例的胞质含红色嗜酸性颗粒的髓细胞样瘤细胞与成淋巴细胞,排列致密呈局灶性生长,正常的组织细胞被挤压或破坏。超微病理观察,在脾脏和法氏囊等组织中均看到病毒颗粒,有囊膜,直径约100nm。部分淋巴细胞核膜、细胞膜水肿或溶解破裂,线粒体和内质网也水肿或池变大、细胞质中出现很多空泡状结构。肿瘤细胞的增多以及细胞内和细胞间的水肿,可能是各脏器严重肿大的主要原因。  相似文献   

14.
An eight-year-old neutered male domestic shorthaired cat was presented with non-weightbearing lameness of the right hindlimb. On radiography, a pathological, metaphyseal femoral fracture was apparent, with areas of bony lysis directly surrounding the fracture site. The cat underwent amputation of the limb. Histopathological examination revealed the presence of a tumour of mesenchymal cells with cytoplasm-containing vacuoles which stained positive with the periodic acid-schiff reaction, indicative of glycogen granules that may be found in rhabdomyosarcoma cells. In addition, the tumour cells stained positive for vimentin and desmin on immunohistochemical examination, thus confirming the diagnosis of rhabdomyosarcoma. In spite of the radical surgery performed, the cat was euthanased due to a recurrence at the amputation site four and a half months later.  相似文献   

15.
The clinicopathological and immunohistochemical findings of a primary feline mammary tumour with features similar to human and canine primary inflammatory carcinoma are described for the first time. The cat presented to the clinic for the rapid onset of oedema, severe erythema, local pain and warmth of the inguinal region, with a pustular-to-nodular cutaneous lesion in association with an ill-defined underlying mass. An epithelial malignant tumour was diagnosed by cytological investigation. Necropsy revealed a thickening of the skin with oedema of the subcutis in the right inguinal area, and regional and distant metastases. Histology showed an unencapsulated tubulopapillary proliferation of malignant epithelial cells, with a massive embolisation in the dermal lymphatics and a mild inflammatory infiltrate. Through immunohistochemistry, the tumour was found to be oestrogen (ER)-alpha-, androgen (AR)- and progesterone (PR)-negative; neoplastic cells were ER-alpha, AR-negative and focally PR-positive. An irregular, mild and focal HER-2 immunoreactivity was present (score +1, non-HER-2 overexpressing). The neoplastic cells were cyclo-oxygenase-2 and vascular endothelial growth factor positive.  相似文献   

16.
Cancer relapse following chemotherapy has been attributed in part to the presence of cancer stem cells (CSC), which drive tumour growth and metastasis and are highly resistant to the effects of cytotoxic chemotherapy. As a result, treatment with cytotoxic chemotherapy selects for drug‐resistant CSC populations that eventually drive tumour recurrence. Little is known currently regarding the role of CSC in dogs with lymphoma, nor the impact of chemotherapy on CSC populations. Therefore, we prospectively quantitated CSC populations in dogs with B‐cell (BCL) and T‐cell lymphoma (TCL), using tumour aspirates and flow cytometric analysis with a panel of CSC markers. In addition, in vitro studies were carried out to determine the impact of chemotherapy resistance on the stem cell phenotype and stem cell properties of lymphoma cells. We found that the percentages of tumour cells expressing CSC markers were significantly increased in dogs with BCL, compared with B cells from normal lymph nodes. Similar findings were observed in dogs with TCL. In vitro studies revealed that lymphoma cells selected for resistance to CHOP chemotherapy had significantly upregulated expression of CSC markers, formed spheroids in culture more readily, and expressed significantly greater aldehyde dehydrogenase activity compared with chemotherapy‐sensitive tumour cells. Similar results were observed in tumour samples dogs with relapsed BCL. These findings suggest that cytotoxic chemotherapy can lead to a relative enrichment of tumour cells with CSC properties, which may be associated with lymphoma recurrence.  相似文献   

17.
A granular cell myoblastoma was found in the dorsal side of the tongue of a 9‐year‐old female mongrel Pinscher. Macroscopically the tumour was white, well circumscribed, and 1½> cm in diameter. Microscopically the tumour cells were arranged in small compact nests or cords with delicate, poorly vascularized collagen bundles in between. A cinar structures were found, also a few mitotic figures were present. Cytoplasmic granules showed a positive periodic acid Schiff reaction. Ultrastructural features included indented nuclei and small‐sized vesicular cytoplasmic granules.

The histogenesis of the neoplasm remains uncertain.  相似文献   

18.
A 14-month-old Yorkshire boar was examined. In the abdomen, two big tumour masses were found in the intestines. In addition, pale nodules occurred in the liver, kidneys, in and upon the spleen, in the wall of the heart and in the testicles. By immunohistochemistry tumour cells were positive for CD3, but negative for B-lymphocyte antigen and CD79. From these findings it was concluded that the boar was suffering from multicentric lymphosarcoma probably of T-cell origin.  相似文献   

19.
Canine endometrial carcinomas are rare, and mostly occur in geriatric bitches. In this work, the uterus of a 10‐year‐old female Boxer evidencing an endometrial carcinoma on the body of the uterus was used to describe the histopathological features of the tumour and to study its immunophenotype. In this work, a panel of immunomarkers (cytokeratins AE1/AE3 and 14, vimentin, CD10 and Ki‐67) was applied to the endometrial carcinoma to establish the staining patterns indicative of the tumour agressiveness and cellular differentiation. Additionally DNA ploidy was also performed. In this case, the tumour showed papillar pattern, with large pleomorphic, anaplastic cells and also some aberrant multinucleated and giant cells. In some areas of the tumour, it was also observed cytotrophoblastic‐like cells outlining the papillae. Cytokeratin AE1/AE3 expression was detected in the luminal neoplasic cells. Cytokeratin 14 positivity was sporadic and irregular, and was observed mainly in the luminal epithelium. Only stromal and aberrant cells showed a positive staining to vimentin. Positive membranous staining to CD10 was evidenced by clear epithelial, cytotrophoblastic‐like cells at the tumour surface but not by the stromal cells. The mitotic and Ki‐67 indices were low, suggestive of a weak aggressiveness of the tumour. The multinucleated and giant cells evidenced a positive immunostaining to CK AE1/AE3, and CD 10; its positivity to vimentin was sporadic. This study aims to contribute to the advancement of the knowledge in canine endometrial carcinoma immunophenotype.  相似文献   

20.
A histological grading system of oesophageal sarcomas has not been established. Thirty-two cases of oesophageal sarcomas have been reviewed for tumour characteristics and clinical outcome. Nineteen dogs underwent surgical intervention to remove oesophageal tumours; ten of them survived (median 278 days). Primary tumour types included osteosarcoma (47%), osteosarcoma with tumour giant cells (7%), fibroblastic osteosarcoma (13%), chondroblastic osteosarcoma (7%) fibrosarcoma (23%) and undifferentiated sarcoma (3%). Histological grade evaluation revealed 33% grade 1 sarcoma, 50% grade 2 and 17% grade 3. No correlation could be found between survival and signalment, duration of clinical signs, tumour type, tumour grade and chemotherapy. Chemotherapy was found to reduce lung metastases' histological scores in three cases (P=0.0007). Surgery seems to be the treatment of choice but the effect of chemotherapy warrants further investigation. Additional research of cases should be performed in order to further define prognostic factors of oesophageal sarcomas.  相似文献   

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