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中国野生花卉的开发及产业可持续发展探讨 总被引：7，自引：1，他引：6
中国有着丰富的野生花卉资源,保护和合理开发利用野生花卉资源是实现花卉产业可持续发展的物质基础.现介绍野生花卉种质资源研究的新技术新方法,阐述野生花卉引种驯化和开发利用情况及我国花卉产业可持续发展,并就存在的问题和发展前景提出了一些建议. 相似文献
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园林对城市环境具有重要的调节作用,可净化城市空气,改善居住环境,缓解城市污染问题,而野生花卉是园林建筑发挥调节作用的关键,因此,必须重视野生花卉的作用,进行科学合理的野生花卉造景与野生花卉配置,以保证园林的实效性。为此,分析了现行野生花卉造景与野生花卉配置模式中存在的不足,提出了野生花卉造景与野生花卉配置应遵循的基本原则,并围绕基本原则提出了具体的改进措施,以推进我国园林可持续发展。 相似文献
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韭菜害虫的危害与防治 总被引：1，自引：0，他引：1
韭菜害虫的危害与防治杨有权，吴慧杰，刘晶（吉林省蔬菜花卉科学研究所）韭菜（ＡｌｌｉｉｕｍａｍｐｅｌｏｐｒａｓｕｍＬ）属于百合科葱属多年生宿根作物。原产亚州。野生韭菜遍布我国的许多荒山野岭。我国栽培韭菜已有三千多年的历史。韭菜是我国特有的传统蔬菜，风味... 相似文献
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野生花卉以其突出的自然和地域特色,在生态景观建设中具备生态、经济、美学价值,但随着农业生产、环境气候变化、人为干扰等各方面的影响,野生花卉的数量急剧下降,宣传应用与保护工作迫在眉睫.为促进野生花卉在国内园林景观的研究与推广,总结了野生花卉在国内外的应用方式,提出了野生花卉的保护与应用的价值,提出中国对野生花卉资源仍停留... 相似文献
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RAPD技术在石榴品种分类上的应用 总被引：5，自引：1，他引：5
以55个石榴栽培品种为试验材料,利用15条多态性好的随机引物进行RAPD分析,共扩增出125条带,其中多态性条带92条,多态性百分率73.6%,说明品种间有变异。应用TFPGA软件计算55个石榴品种间的Nei’s遗传距离,并用UPGMA法构建聚类图,将55个石榴品种分为4个类群,从DNA水平上揭示了石榴品种之间的亲缘关系。结果表明,采用UPGMA法聚类的结果与形态上的分类存在着一定的差异,即与根据花色和果味进行的分类没有相关性而与瓣型进行的分类有一定的相关性。 相似文献
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Process-based forest landscape models are valuable tools for testing basic ecological theory and for projecting how forest
landscapes may respond to climate change and other environmental shifts. However, the ability of these models to accurately
predict environmentally-induced shifts in species distributions as well as changes in forest composition and structure is
often contingent on the phenomenological representation of individual-level processes accurately scaling-up to landscape-level
community dynamics. We use a spatially explicit landscape forest model (LandClim) to examine how three alternative formulations
of individual tree growth (logistic, Gompertz, and von Bertalanffy) influence model results. Interactions between growth models
and landscape characteristics (landscape heterogeneity and disturbance intensity) were tested to determine in what type of
landscape simulation results were most sensitive to growth model structure. We found that simulation results were robust to
growth function formulation when the results were assessed at a large spatial extent (landscape) and when coarse response
variables, such as total forest biomass, were examined. However, results diverged when more detailed response variables, such
as species composition within elevation bands, were considered. These differences were particularly prevalent in regions that
included environmental transition zones where forest composition is strongly driven by growth-dependent competition. We found
that neither landscape heterogeneity nor the intensity of landscape disturbances accentuated simulation sensitivity to growth
model formulation. Our results indicate that at the landscape extent, simulation results are robust, but the reliability of
model results at a finer resolution depends critically on accurate tree growth functions. 相似文献
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以生产收集的24个黑木耳栽培菌株为试材,采用拮抗、酯酶同工酶及ISSR分子标记技术对其进行分类鉴定和遗传多样性分析。结果表明:酯酶同工酶酶谱显示24个黑木耳菌株共扩增出11条迁移率不同的酶带,聚类分析表明当遗传系数为0.74时,将24个黑木耳菌株分为两大类;通过ISSR分子标记共扩增出67条清晰的DNA多态片段,大小介于0.2～2.0 kb,聚类分析表明当遗传系数为0.85时,将24个菌株分为两大类,聚类分析结果与拮抗试验结果基本一致。该研究为黑木耳的菌株选择及遗传育种提供了参考依据。 相似文献
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大棚蔬菜连作对土壤多糖影响的研究 总被引：2，自引：0，他引：2
研究了大棚蔬菜作物连作条件下对土壤多糖的影响,测定了不同蔬菜连作土壤的多糖含量,并对土壤多糖的提取方法进行了优化。结果表明:对土壤样品进行6 h沸水浴处理,H2SO4浓度为2.0 mol/L条件下,多糖的提取效果最好;蔬菜连作土壤多糖含量的测定表明,连作4~5 a的大棚土壤多糖含量明显低于对照;种植叶菜类土壤的多糖含量高于种植果菜类土壤的含量,并且差异达到显著水平。 相似文献
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2016—2018年以洛阳地区不同牡丹种植地的根腐病株为试材,采用组织分离法进行根腐病病原菌的分离纯化,根据柯赫氏法则检测分离物的致病性,同时结合形态学特征和基因序列分析对具有致病性的分离物进行鉴定,以明确引起洛阳地区牡丹根腐病发病的主要病原菌种类。结果表明:从30份根腐病株中共分离获得15株病原分离物,离体致病性检测表明9株分离物具有显著致病性;根据形态学特征,初步鉴定9株致病分离物为镰刀菌属(Fusarium)真菌,rDNA-ITS序列和EF-1α基因序列分析表明其中7株致病分离物为茄病镰刀菌(Fusarium solani),2株致病分离物为尖孢镰刀菌(Fusarium oxysporum)。因此,该研究结果初步表明茄病镰刀菌和尖孢镰刀菌是引发洛阳地区牡丹根腐病的主要病原菌种类,2种病原菌复合侵染引起根腐病的发生,其中茄病镰刀菌为优势致病菌。 相似文献
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牡丹89 个不同种源品种遗传多样性和亲缘关系分析 总被引：2，自引：0，他引：2
利用ISSR分子标记技术,从121条ISSR备选引物中筛选出条带清晰、多态性好的19条引物对89个牡丹品种的遗传多样性进行了分析。通过优化PCR试验条件,共获得188条带,其中177条表现出多态性,多态位点的百分率为94.1%。通过计算机软件分析,供试牡丹品种平均有效等位基因数为1.514,平均Nei’s基因多样性指数为0.3085,平均Shannon’s信息指数为0.4713;各品种间的相似系数介于0.3294 ~ 0.7744之间,平均为0.5722。利用UPGMA法作图,供试的89个品种可聚为2个类群,很好地将不同来源的品种区分开来。 相似文献
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AIM: To investigate liquid-based cytology technique (LCT) combined with high-risk human papilloma virus DNA (HPV-DNA) detection for diagnosis of cervical precancerous lesions. METHODS: Screening of cervical cancer was performed by LCT combined with HPV-DNA detection in 6 521 women at the age of 19~65 in our hospital for physical examination from December 2007 to December 2010. Eighteen high-risk HPV isoform genes were detected by HPV typing gene chip detection system. The women with positive detection underwent colposcopic cervical biopsies. The women with negative detection also underwent colposcopic cervical biopsies if the operation was of their own accord. RESULTS: The LCT positive results (≥ASCUS) were observed in 152 cases and HPV positive results were obtained in 86 cases. The women with both 2 positive results were found in 42 cases. The women with both LCT and HPV negative results were determined in 6 325 cases. In 152 cases of LCT positive and 86 cases of HPV positive women, the positive results of pathological biopsy (≥CINI) were 112 and 68 cases, respectively. Thirty-four cases in the 42 cases of both LCT and HPV positive women were conformed by pathological biopsy. In 6 325 women with negative results of LCT and HPV detection, 2 000 were perform pathological biopsy according to their own accord and only 1 had positive result. The diagnostic sensitivity of LCT for cervical precancerous lesions was 76.19% with the specificity of 98.05%. The diagnostic sensitivity of HPV detection for precancerous lesions of uterine cervix was 46.26% with the specificity of 99.12%. Combination of the 2 methods to diagnose cervical precancerous lesions made the sensitivity increase to 99.32% with the specificity of 99.61%. CONCLUSION: Liquid-based cytology technique combined with high-risk HPV-DNA detection is better than each of the single technique for screening precancerous lesion of cervical carcinoma. 相似文献
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AIM: To screen the chemotherapy resistance-related microRNAs (miRNAs) of colon cancer using gene chip technique, and to explore the mechanism of miRNAs regulating chemotherapy resistance. METHODS: Gene chip technique was used to analyze the expression of miRNAs in colon cancer cell line HCT8 and vincristine-resistant cell line HCT8/v, and screen the miRNAs with significantly different expression. The results were verified by RT-qPCR. The target genes of these miRNAs were predicted, and the Gene Ontology (GO) analysis and the signaling pathway analysis of the predicted genes were carried out. RESULTS: Altogether 342 miRNAs with significantly differential expression were selected, in which 190 were up-regulated, and 152 were down-regulated. The verification results of RT-qPCR showed that the expression of miR-125-5p, miR-181c-5p and miR-153-3 was consistent with the results of chip detection. The expression of miR-130a-3p and miR-149-3p was not consistent with the results of chip detection. The results of GO analysis showed that the main pathway of chemotherapy resistance-related genes was RNA polymerase II regulatory region sequence-specific DNA binding. The chemotherapy resistance-related genes played roles mainly through positive regulation and are mainly located in intracellular membrane-bound organelles. The results of KEGG analysis showed that the pathways associated with the most enriched chemotherapy resistance-related genes were axon guidance pathway, insulin signaling pathway, and phospholipase D signaling pathway.CONCLUSION: miRNAs are closely related to chemotherapy resistance in colon cancer. Through the researches on miRNAs, we can have a deeper understanding of the mechanism of chemotherapy resistance and provide new ideas for reversing chemotherapy resistance in colon cancer. 相似文献
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几种冬青属植物抗冻能力比较 总被引：17，自引：0，他引：17
选取了11种国内外冬青园艺品种或种, 以其离体叶片为试材, 采用低温循环仪自动降温程序模拟冰冻处理, 用电导法测定相对电导率, 并配以Logistic方程, 计算出各自的半致死温度。试验结果显示11种冬青的抗冻能力由高到低依次为: 龟甲冬青( Ilex crenata var. convexa) 、绿冬青( I. viridis) 、拉索里娜冬青( I. ×altaclerensi‘Lawsoniana’) 、枸骨( I.cornuta) 、冬青( I. chinensis) 、无刺枸骨( I. cornuta var. fortunei) 、金边小叶冬青( I. ×altaclerensis ‘Golden Van Tol’) 、毛冬青( I. pubescens) 、花刺冬青( I. aferox ‘Avgentea’) 、小果冬青( I. micrococca) 和金宝石冬青( I. crenata ‘Golden Gem’) 。在此基础上选出9种冬青树种离体叶片为试材, 作多个低温处理, 测定其可溶性糖和游离脯氨酸含量。结果表明, 随处理温度的降低, 可溶性糖和游离脯氨酸的含量逐渐增加, 低温下可溶性糖和游离脯氨酸的含量仅与部分冬青的抗冻性呈正相关。该研究数据对推测引进新品种的抗冻能力, 为它们的合理应用提供科学依据。 相似文献