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1.
Mapping of the human Blym-1 transforming gene activated in Burkitt lymphomas to chromosome 1 总被引：7，自引：0，他引：7
C C Morton R Taub A Diamond M A Lane G M Cooper P Leder 《Science (New York, N.Y.)》1984,223(4632):173-175
Blym-1, a transforming gene detected by transfection of NIH 3T3 cells with DNA from Burkitt lymphomas, was mapped to the short arm of chromosome 1 (1p32) by chromosomal in situ hybridization. The Blym-1 gene was not physically linked to the cellular myc oncogene or to any of the immunoglobulin gene loci implicated in the characteristic chromosomal translocations in Burkitt lymphoma. 相似文献
2.
Gene for human insulin receptor: localization to site on chromosome 19 involved in pre-B-cell leukemia 总被引：15，自引：0，他引：15
Consistent chromosomal translocations in neoplastic cells may alter the expression of proto-oncogenes that are located near the breakpoints. The complementary DNA sequence of the human insulin receptor is similar to those of the EGF receptor (erbB oncogene) and products of the src family of oncogenes. With in situ hybridization and Southern blot analysis of somatic cell hybrid DNA, the human insulin receptor gene was mapped to the distal short arm of chromosome 19 (bands p13.2----p13.3), a site involved in a nonrandom translocation in pre-B-cell acute leukemia. 相似文献
3.
Construction of a general human chromosome jumping library, with application to cystic fibrosis 总被引：22，自引：0，他引：22
F S Collins M L Drumm J L Cole W K Lockwood G F Vande Woude M C Iannuzzi 《Science (New York, N.Y.)》1987,235(4792):1046-1049
In many genetic disorders, the responsible gene and its protein product are unknown. The technique known as "reverse genetics," in which chromosomal map positions and genetically linked DNA markers are used to identify and clone such genes, is complicated by the fact that the molecular distances from the closest DNA markers to the gene itself are often too large to traverse by standard cloning techniques. To address this situation, a general human chromosome jumping library was constructed that allows the cloning of DNA sequences approximately 100 kilobases away from any starting point in genomic DNA. As an illustration of its usefulness, this library was searched for a jumping clone, starting at the met oncogene, which is a marker tightly linked to the cystic fibrosis gene that is located on human chromosome 7. Mapping of the new genomic fragment by pulsed field gel electrophoresis confirmed that it resides on chromosome 7 within 240 kilobases downstream of the met gene. The use of chromosome jumping should now be applicable to any genetic locus for which a closely linked DNA marker is available. 相似文献
4.
The role of the c-mos gene in the 8;21 translocation in human acute myeloblastic leukemia 总被引：2，自引：0，他引：2
M O Diaz M M Le Beau J D Rowley H A Drabkin D Patterson 《Science (New York, N.Y.)》1985,229(4715):767-769
The human c-mos proto-oncogene is located on chromosome 8 at band q22, close to the breakpoint in the t(8;21) (q22;q22) chromosome rearrangement. This translocation is associated with acute myeloblastic leukemia, subgroup M2. The c-myc gene, another proto-oncogene, has been mapped to 8q24. The breakpoint at 8q22 separates these genes, as determined by in situ hybridization of c-mos and c-myc probes. The c-mos gene remains on the 8q-chromosome and the c-myc gene is translocated to the 21q+ chromosome. Southern blot analysis of DNA from bone marrow cells of four patients with this translocation showed no rearrangement of c-mos. 相似文献
5.
L R Finger R C Harvey R C Moore L C Showe C M Croce 《Science (New York, N.Y.)》1986,234(4779):982-985
The chromosomal breakpoint involved in the t(8;14)(q24;q11) chromosome translocation in the SKW-3 cell line, which directly involves the 3' flanking region of the c-myc gene, was cloned and sequenced. The breakpoint on chromosome 8 mapped to a position 3 kb 3' of c-myc while the chromosome 14 breakpoint occurred 36 kb 5' of the gene for the constant region of the alpha chain of the T-cell receptor (TCR). The translocation resulted in a precise rearrangement of sequences on chromosome 8 and what appears to be a functional J alpha segment on chromosome 14. Signal sequences for V-J joining occurred at the breakpoint positions on both chromosomes 14 and 8, suggesting that the translocation occurs during TCR gene rearrangement and that it is catalyzed by the enzymatic systems involved in V-J joining reactions. The involvement of c-myc in the translocation and the association of joining signals at the breakpoints provides a parallel to the situation observed in the translocations involving c-myc and the immunoglobulin loci in B-cell neoplasms and suggests that common mechanisms of translocation and oncogene deregulation are involved in B- and T-cell malignancies. 相似文献
6.
High-resolution chromosome sorting and DNA spot-blot analysis assign McArdle's syndrome to chromosome 11 总被引：23，自引：0，他引：23
R V Lebo F Gorin R J Fletterick F T Kao M C Cheung B D Bruce Y W Kan 《Science (New York, N.Y.)》1984,225(4657):57-59
A rapid gene-mapping system uses a high-resolution, dual-laser sorter to identify genes from separate human chromosomes prepared with a new stain combination. This system was used to sort 21 unique chromosome types onto nitrocellulose filter papers. Several labeled gene probes hybridized to the sorted chromosomal DNA types predicted by their previous chromosome assignments. The skeletal muscle glycogen phosphorylase gene was then mapped to a portion of chromosome 11 by spot blotting normal and translocated chromosomes. 相似文献
7.
Human c-Ki-ras2 proto-oncogene on chromosome 12 总被引：11，自引：0，他引：11
A Y Sakaguchi S L Naylor T B Shows J J Toole M McCoy R A Weinberg 《Science (New York, N.Y.)》1983,219(4588):1081-1083
A human colonic adenocarcinoma transforming gene, recently identified as a cellular homolog of the Kirsten sarcoma gene (v-ras), was used to assign the human cellular Kirsten ras2 gene to chromosome 12 by the Southern hybridization method. A single 640 base-pair Eco RI--Hind III fragment of the transforming gene, isolated by DNA transfection and molecular cloning, can detect a single Eco RI fragment (2.9 kilobase pairs) of DNA from phenotypically normal cells. The data suggest a constant chromosomal location of c-Ki-ras2. 相似文献
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9.
Chromosomal location of human T-cell receptor gene Ti beta 总被引：10，自引：0，他引：10
P E Barker F H Ruddle H D Royer O Acuto E L Reinherz 《Science (New York, N.Y.)》1984,226(4672):348-349
A complementary DNA probe corresponding to the beta-chain gene of Ti, the human T lymphocyte receptor, has been molecularly cloned. The chromosomal origin of the Ti beta gene was determined with the complementary DNA by screening a series of 12 cell hybrid (mouse X human) DNA's containing overlapping subsets of human chromosomes. DNA hybridization (Southern) experiments showed that the human Ti beta gene resides on chromosome 7 and is thus not linked to the immunoglobulin loci or to the major histocompatibility locus in humans. 相似文献
10.
Chromosomal localization of the human homolog (c-sis) of the simian sarcoma virus onc gene 总被引：34，自引：0，他引：34
Nonrandom chromosome rearrangements of chromosome 22 have been identified in different human malignancies. As a result of Southern blot hybridization of a c-sis probe to DNA's from mouse-human somatic cell hybrids, the human homolog (c-sis) of the transforming gene of simian sarcoma virus was assigned to chromosome 22. Hybrids between thymidine kinase-deficient mouse cells and human fibroblasts carrying a translocation of the region q11-qter of chromosome 22 to chromosome 17 were also analyzed. These studies demonstrate that the human c-sis gene is on region 22q11 greater than qter. 相似文献
11.
U Kasid A Pfeifer R R Weichselbaum A Dritschilo G E Mark 《Science (New York, N.Y.)》1987,237(4818):1039-1041
12.
High-resolution mapping of human chromosome 11 by in situ hybridization with cosmid clones 总被引：133，自引：0，他引：133
P Lichter C J Tang K Call G Hermanson G A Evans D Housman D C Ward 《Science (New York, N.Y.)》1990,247(4938):64-69
Cosmid clones containing human DNA inserts have been mapped on chromosome 11 by fluorescence in situ hybridization under conditions that suppress signal from repetitive DNA sequences. Thirteen known genes, one chromosome 11-specific DNA repeat, and 36 random clones were analyzed. High-resolution mapping was facilitated by using digital imaging microscopy and by analyzing extended (prometaphase) chromosomes. The map coordinates established by in situ hybridization showed a one to one correspondence with those determined by Southern (DNA) blot analysis of hybrid cell lines containing fragments of chromosome 11. Furthermore, by hybridizing three or more cosmids simultaneously, gene order on the chromosome could be established unequivocally. These results demonstrate the feasibility of rapidly producing high-resolution maps of human chromosomes by in situ hybridization. 相似文献
13.
B Rajput S F Degen E Reich E K Waller J Axelrod R L Eddy T B Shows 《Science (New York, N.Y.)》1985,230(4726):672-674
A panel of human-mouse somatic cell hybrids and specific complementary DNA probes were used to map the human tissue plasminogen activator and urokinase genes to human chromosomes 8 and 10, respectively. This result is in contrast to a previous assignment of a plasminogen activator gene to chromosome 6. As neoplastic cells produce high levels of plasminogen activator, it is of interest that aberrations of chromosome 8 have been linked to various leukemias and lymphomas and that two human oncogenes, c-mos and c-myc, have also been mapped to chromosome 8. 相似文献
14.
Gene for T-cell growth factor: location on human chromosome 4q and feline chromosome B1 总被引：8，自引：0，他引：8
L J Seigel M E Harper F Wong-Staal R C Gallo W G Nash S J O'Brien 《Science (New York, N.Y.)》1984,223(4632):175-178
T-cell growth factor (TCGF) or interleukin-2 (IL-2), an immunoregulatory lymphokine, is produced by lectin- or antigen-activated mature T lymphocytes and in a constitutive manner by certain T-cell lymphoma cell lines. By means of a molecular clone of human TCGF and DNA extracted from a panel of somatic cell hybrids (rodent cells X normal human lymphocytes), the TCGF structural gene was identified on human chromosome 4. In situ hybridization of the TCGF clone to human chromosomes resulted in significant labeling of the midportion of the long arm of chromosome 4, indicating that the TCGF gene was located at band q26-28. Genomic DNA from a panel of hybrids prepared with HUT-102 B2 cells was examined with the same molecular clone. In this clone of cells, which produces human T-cell leukemia virus, the TCGF gene was also located on chromosome 4 and was apparently not rearranged. The homologous TCGF locus in the domestic cat was assigned to chromosome B1 by using a somatic cell hybrid panel that segregates cat chromosomes. Linkage studies as well as high-resolution G-trypsin banding indicate that this feline chromosome is partially homologous to human chromosome 4. 相似文献
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16.
A new cluster of genes within the human major histocompatibility complex 总被引：29，自引：0，他引：29
T Spies G Blanck M Bresnahan J Sands J L Strominger 《Science (New York, N.Y.)》1989,243(4888):214-217
17.
The chromosomal basis of human neoplasia 总被引：85，自引：0，他引：85
J J Yunis 《Science (New York, N.Y.)》1983,221(4607):227-236
High-resolution banding techniques for the study of human chromosomes have revealed that the malignant cells of most tumors analyzed have characteristic chromosomal defects. Translocations of the same chromosome segments with precise breakpoints occur in many leukemias and lymphomas, and a specific chromosome band is deleted in several carcinomas. Trisomy, or the occurrence of a particular chromosome in triplicate, is the only abnormality observed in a few neoplasias. It is proposed that chromosomal rearrangements play a central role in human neoplasia and may exert their effects through related genomic mechanisms. Thus, a translocation could serve to place an oncogene next to an activating DNA sequence, a deletion to eliminate an oncogene repressor, and trisomy to carry extra gene dosage. 相似文献
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19.
Concerted nonsyntenic allelic loss in human colorectal carcinoma 总被引：12，自引：0，他引：12
D J Law S Olschwang J P Monpezat D Lefran?ois D Jagelman N J Petrelli G Thomas A P Feinberg 《Science (New York, N.Y.)》1988,241(4868):961-965
Familial polyposis coli (FPC) is caused by an autosomal dominant gene on chromosome 5, and it has been proposed that colorectal cancer in the general population arises from loss or inactivation of the FPC gene, analogous to recessive tumor genes in retinoblastoma and Wilms' tumor. Since allelic loss can be erroneously scored in nonhomogeneous samples, tumor cell populations were first microdissected from 24 colorectal carcinomas, an additional nine cancers were engrafted in nude mice, and nuclei were flow-sorted from an additional two. Of 31 cancers informative for chromosome 5 markers, only 6 (19%) showed loss of heterozygosity of chromosome 5 alleles, compared to 19 of 34 (56%) on chromosome 17, and 17 of 33 (52%) on chromosome 18. Therefore, it appears that (i) FPC is a true dominant for adenomatosis but not a common recessive gene for colon cancer; and (ii) simple Mendelian models involving loss of alleles at a single locus may be inappropriate for understanding common human solid tumors. 相似文献
20.
在已知萝卜D染色体附加系对线虫具有抗性和萝卜分子图谱的7号连锁群定位有抗线虫基因Hs1Raph的基础上,利用(dp)RAPD分子标记技术,以甘蓝型油菜异源萝卜附加系为材料,筛选到90个萝卜D染色体的特异标记,选择4个在萝卜构图亲本间表现多态性的D染色体标记进行F2群体(包括245个单株)分离检测,并与构图用的505个AFLP和RAPD标记一起用Join-map3.0进行连锁定位分析。结果萝卜D染色体标记全部定位于7号连锁群,并且7号连锁群上只有D染色体的特异标记,说明萝卜7号连锁群就是D染色体在分子水平的反映。证实了定位有抗线虫基因Hs1Raph的7号连锁群对应于具线虫抗性效应的萝卜D染色体。实验结果对研究萝卜D染色体控制的农艺性状或抗逆性以及萝卜抗线虫育种和将萝卜抗线虫基因转入其它芸薹属作物的研究都将会起到重要的指导作用。 相似文献