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1.
Two worm-free calves were allowed to graze on irrigated pasture with a naturally infected herd for each of 34 one-month periods from November 1979 to August 1982. After each grazing period, the calves were transferred to a cement-floored pen for 3 weeks and then were euthanatized and necropsied. Ostertagia ostertagi and Cooperia oncophora were the most prevalent species of nematodes recovered. Adults and larvae of Ostertagia spp and Cooperia spp were most numerous in winter and spring and least numerous during summer. The proportions of Ostertagia spp that were inhibited as fourth-stage larvae increased in late fall, peaked from March through April, and then decreased to low values during summer. The maximal inhibition in 1980, 1981, and 1982 was 72, 65, and 62%, respectively. The number of larval Cooperia spp was highest in winter months and, except for one grazing period when 55% of the Cooperia spp were larvae, the total numbers represented less than 15% of the nematode population during all grazing periods. Other nematodes encountered were Trichostrongylus axei, Haemonchus spp, O lyrata, and O occidentalis in the abomasum; C surnabata, C punctata, Nematodirus helvetianus, T colubriformis, and Bunostomum phlebotomum in the small intestine; and Oesophagostomum venulosum and Trichuris ovis in the large intestine.  相似文献   

2.
A study was initiated to (1) determine species of gastrointestinal nematodes present in cattle in selected areas of Oregon, (2) examine seasonal abundance of these nematodes as an indicator of periods of transmission, and (3) determine when developmental inhibition occurs in the major genera of nematodes encountered. Four study sites were chosen: Corvallis, Langlois, near Fort Rock, and southeast of Klamath Falls on the Oregon-California border. Eight sets of three to four tracer calves each were introduced onto pasture at each site over a 2 year period with time of turn out onto pasture corresponding to late spring, mid-summer, late fall and late winter. At Corvallis, a distinct seasonality in parasite transmission was evident, peaking during the fall and winter. At Langlois, transmission was fairly constant throughout the year. No discernible patterns were evident at either Klamath Falls or Fort Rock. Eight nematode genera were found during the study. Ostertagia, Cooperia, Nematodirus and Trichostrongylus were present at all study sites and were the most common genera at each. Trichuris was found at all sites except Klamath Falls. Oesophagostomum was present in tracers only from Langlois and Corvallis while Haemonchus was found only at Klamath Falls and Fort Rock. Capillaria was only present at Klamath Falls. Where possible, specific transmission patterns for Nematodirus, Cooperia, and Ostertagia were determined for each site. Nematodirus was transmitted fairly steadily at both Langlois and Corvallis but was quite variable at Fort Rock. Developmental arrest was detected in this genus at all study sites during the fall and/or winter. Cooperia exhibited the most seasonally defined pattern of transmission, peaking during the fall and winter at Langlois, Corvallis and Klamath Falls. Hypobiotic larvae of Cooperia were present during the fall and/or winter only at Langlois and Corvallis. Peak transmission of Ostertagia at Langlois and Corvallis occurred during the fall and winter. At Fort Rock, transmission was lowest in the fall and increased in the winter. Hypobiotic larvae were evident in the fall and winter at Corvallis, Fort Rock and Klamath Falls. These data suggest Type II ostertagiasis may occur in late winter through spring in these areas. Hypobiotic larvae of Ostertagia were not detected at Langlois. The lack of appropriate environmental stimuli is one possible explanation for the apparent lack of hypobiosis at that site.  相似文献   

3.
The occurrence and seasonal trends of nematode parasite infections in beef cattle on the Tablelands and North Coast regions of New South Wales are described, based on worm counts from 627 spring-born steers slaughtered at 2-monthly intervals from 6 to 24 months of age. The predominant parasites were Ostertagia ostertagi, Trichostrongylus axei and Cooperia spp. Large burdens of adult Ostertagia were seen in 12-month-old animals in late winter and early spring, and later in 18- to 20-month-old cattle in the following late summer and autumn. These infections often resulted in outbreaks of clinical parasitism. Massive numbers of inhibited early fourth stage larvae (EL4) also accumulated in the yearlings during their first spring, reached peaks in mid-summer and then declined. The possibility of their resumption of development to mature worms in the late summer and autumn period is discussed. Adult T. axei showed a similar seasonal trend to Ostertagia and may have increased the severity of outbreaks of clinical disease. Cooperia populations of EL4 and adult worms were highest in weaners during winter, but lower numbers thereafter indicated a strong resistance to re-infection. In addition, Haemonchus placei occurred frequently on the North Coast both as EL4 and adults in cattle of all ages up to 20 months. Of the other cattle nematodes, Oesophagostomum radiatum and Trichuris spp occurred in low numbers, mainly in weaners. Bunstomum phlebotomum and H. contortus occurred sporadically on the North Coast and Tablelands respectively. Intestinal Trichostronglyus spp, Oesophagostomum venulosum and Dictyocaulus viviparus were seen occasionally and Nematodirus spp were not seen. There was a poor relationship between worm counts and faecal egg counts. Cooperia spp dominated the egg counts, while those for other genera were generally low and did not reflect the relative abundance or seasonal changes in worm numbers.  相似文献   

4.
The patterns of gastrointestinal and pulmonary nematode infections in a previously untreated Aberdeen Angus cow-calf herd were observed between May 1988 and December 1990. The cow-calf herd and replacement heifers were on separate pastures. The relatively high mean faecal egg counts of cows and heifers at the time of turnout were mainly owing to the maturation of hypobiotic worms. The strongyle egg counts of calves began to rise soon after turnout onto pasture and reached peak levels at the end of the grazing season. The number of infective larvae on pasture was highest during September/October. Ostertagia, Cooperia and Nematodirus were the most prevalent genera found at necropsy and on pasture. Larvae of these nematodes were able to overwinter on pasture and Ostertagia larvae, additionally, were able to overwinter in the host as arrested early fourth stage larvae. The high egg output of cows at the time of turnout may serve as a source of infection for their calves and be responsible for the late-season rise in pasture larval counts.  相似文献   

5.
An epidemiological study of gastrointestinal nematode parasitism in beef cattle in mountainous areas of Spain was performed. The dynamics of contamination with gastrointestinal nematode larvae of Pyrenean pastures was studied over four years at five areas at different altitudes (900 m to 2100 m), grazed by animals according to traditional systems of beef cattle in mountainous areas. Grass samples were taken every two weeks and larval differentiation was performed. Worm egg counts of grazing animals were assessed in cows, heifers and calves. A consistent seasonal pattern of infective larvae on pasture through the study was observed. In hay meadows, located below 1000 m, infective larvae were found from the end of October until June of the following year. At higher altitudes (1200-2100 m), a bimodal pattern of pasture larvae contamination was observed with increases in late spring (March-June) and in late autumn (September-November). Ostertagia spp., Cooperia spp., Trichostrongylus spp., Oesophagostomum spp., and Nematodirus spp. were found, with Ostertagia spp. being the most frequently found, followed by Cooperia spp. The highest increase of larval contamination in autumn coincided with the grazing of animals in hay meadows. This elevated autumn larval population had a very important epidemiological role because these larvae remained as overwintered larvae until the following grazing season, starting the cycle of contamination of the animals.  相似文献   

6.
The patterns of gastrointestinal and lung helminth infections in grazing calves were studied in two small grazing experiments carried out between May and October in 1985 and 1986, respectively, at Utrecht State University. For lungworm a very rapid translation of infective larvae was seen in both experiments, resulting in a rapid build-up of the worm burdens, and in July the second generation of Dictyocaulus caused husk. After the end of patency for lungworm, in the beginning of August, a rapid decrease in pasture infectivity, measured by pasture larval counts and worm counts of tracer calves, was observed. For the gastrointestinal nematodes the translation of infective larvae was much slower as a distinct 'midsummer increase' of pasture infectivity was not seen before the end of July. The worm counts of the tracer calves and the pasture larval counts showed that these larvae remained on pasture much longer than the lungworm larvae. As in Dictyocaulus, patency stopped in August-September in Nematodirus but not in Ostertagia and Cooperia, indicating a slower rate of development of immunity in both latter genera. In contrast to lungworm, the results indicate a clear and predictable seasonal pattern for gastrointestinal helminth infections.  相似文献   

7.
Several steers, reared in isolation until approximately six months of age, were placed on a small isolated enclosed pasture from late spring to late fall of 1970, 1971 and 1972. The pasture was left vacant and unused during the winters and early springs. The pasture had been used in previous years by cattle, and in the late spring of 1970 was grazed by cattle know to be passing gastrointestinal nematode eggs in their feces. The steers were slaughtered periodically, and the prevalence of nematode species in the abomasum, small intestine, and large intestine, was determined from random samples of up to 100 adult male worms from each segment. The following nematodes were found in the steers in 1971 and 1972 and survived winters on pasture: Ostertagia ostertagi, O. lyrata, Cooperia oncophora, C. mcmasteri, Nematodirus helvetianus and Trichuris discolor. Two nematodes Cooperia punctata and Bunostomum phlebotomum known to be present on pasture in 1970, since they were recovered from the steers in that year, were not found in the steers in 1971 and 1972.  相似文献   

8.
Trends in the numbers of infective nematode larvae on pasture plots contaminated by cattle at different seasons of the year were defined in 3 different climatic regions. The main nematodes were Ostertagia ostertagi, Trichostrongylus spp, Haemonchus spp and Cooperia spp.
On the North Coast of New South Wales with a sub-tropical climate, the numbers of infective larvae of all 4 nematodes rose rapidly to peak levels soon after each seasonal period of contamination began, then fell quickly within a few months. On the Central Coast of New South Wales, the trends were similar to those on the North Coast, except that the larvae persisted on the pasture for a much longer time. On the Northern Tablelands of New South Wales, where temperatures were much colder than on the coast, larval development was slower and major peaks of larval availability did not occur until early spring. These different seasonal trends in each region were considered to be related to the climatic differences between the regions.
On pastures which were contaminated continuously, larval numbers reached maximum levels in mid-winter on the Central Coast and in early spring on the Northern Tablelands. It was concluded that the majority of these larvae were derived from the contamination of pastures in autumn and winter. Subsequently in summer, a rapid dying out of larvae was observed in all the regions, probably due to the effect of hotter weather.
The studies suggest that a reduction in the contamination of pasture with nematode eggs in autumn and winter could result in pastures carrying fewer larvae and thus form the basis of effective worm control programs for cattle.  相似文献   

9.
The anthelmintic efficacy of abamectin (avermectin B1) was evaluated against gastrointestinal nematodes, including Ostertagia ostertagi inhibited larvae and lungworm, in yearling crossbred beef heifers during late spring. The calves were grazed on contaminated pasture for 10 weeks and then held under conditions free of nematode infection for 3 weeks prior to allotment and treatment on 5 June. Thirteen calves were randomly assigned to two groups of six by restricted randomization on body weights; the extra lightest calf was assigned to the non-treated control group. Group 1 calves were treated with abamectin at 200 micrograms kg-1 body weight by s.c. injection and Group 2 calves were not treated; all were killed at 14 days after treatment. Ostertagia ostertagi was present in all controls; arithmetic mean numbers of adults, developing fourth stage larvae (L4) and inhibited EL4 were 7683, 605 and 36,102, respectively. Other nematode genera present in controls in sufficient numbers for the experiment were Haemonchus placei adults, Trichostrongylus axei adults, Cooperia spp. adults, Oesophagostomum radiatum adults, Bunostomum phlebotomum adults, Dictyocaulus viviparus adults and E5 (immature adults). Abamectin was highly effective (consistently greater than 99% efficacy and P less than 0.05) in removing all nematodes present in treated calves as represented in non-treated controls, including the primary target of Ostertagia ostertagi inhibited EL4. The lowest efficacy was 93.8%, against D. viviparus E5.  相似文献   

10.
Residual ovine nematode pasture infections were assessed by grazing groups of ewes and their lambs on permanent sheep and cattle pastures and by the use of tracer lambs. Ostertagia spp., Cooperia oncophora, Nematodirus spp., Chabertia ovina and Trichuris spp. eggs and/or larvae survived on pastures overwinter. Second generation Ostertagia larvae were present in greatest numbers on pasture during the latter part of August and early September. The failure of a significant build-up of Cooperia oncophora was attributed to negligible worm egg output of this species in sheep. A build-up of Nematodirus spp. on pasture was not detected in this study.  相似文献   

11.
In a study originally designed to determine the seasonal origin of the high levels of availability of nematode larvae to cattle in winter and spring, plots were serially contaminated with eggs of Ostertagia ostertagi and Cooperia oncophora by naturally-infected calves at monthly intervals from February 1980 to September 1980. The availability of infective larvae was monitored by monthly pasture sampling and larval recovery. Because of the intervention of a 15 month drought, recoveries of larvae from the pastures were very low until March 1981 (autumn in Australia) when large numbers of larvae appeared on pastures contaminated in the preceding spring. Examination of dry dung pats at that time showed that significant numbers of larvae were present in pats deposited up to a year previously, and particularly in pats deposited in May, August and September. Following the resumption of normal rainfall in May 1981, larval numbers in pats rapidly declined and concentrations of larvae on the pastures increased to extremely high levels. It is suggested that survival of infective larvae in dry dung pats was enhanced by the drought, with implications for control of nematode infections of cattle, particularly in winter rainfall environments.  相似文献   

12.
Gastrointestinal nematode parasite burdens were monitored in a herd of field-grazed cattle from weaning to 2 years old to allow observations to be made on the dynamics of burdens of arrested Ostertagia spp. and Cooperia oncophora. Arrested Ostertagia spp. accumulated in the herd over their first autumn and winter to reach a peak in late winter/spring (August-October). From October until late January they declined at a rate comparable to that at which they had accumulated. Numbers continued to decline at a slower rate until the following autumn (May) when they began to increase again. Observations on the decline of worm burdens in a group of animals which had been moved from pasture to worm-free conditions in October indicated that intake of infective larvae over late spring/summer contributed little to the number of arrested Ostertagia spp. in the herd. No clinical Type II ostertagiosis was observed in the field-grazed animals over the time of decline of the arrested worm burden, nor was there an increase in the number of adult Ostertagia spp. present. However, two of the animals removed from pasture to worm-free conditions developed Type II ostertagiosis approximately a month after the move. Worm burdens of successive groups of "tracers" grazed with the herd every 2 months indicated that there was a small seasonal increase in the propensity of Ostertagia spp. for arrested development. However, estimated accumulation rates of arrested larvae calculated from tracer worm burdens, suggest that this seasonal increase in propensity was not adequate in itself to account for the accumulation rate observed in "resident" animals of the herd. No comparable accumulation of arrested C. oncophora took place in resident animals over the first autumn and winter of the trial despite the fact that tracer worm burdens over this period indicated a marked seasonal increase in the propensity for arrested development by this species.  相似文献   

13.
A 4-year study on the free-living stages of cattle gastrointestinal nematodes was conducted to determine (a) the development time from egg to infective larvae (L3) inside the faecal pats, (b) the pasture infectivity levels over time, and (c) the survival of L3 on pasture. Naturally infected calves were allowed to contaminate 16 plots on monthly basis. Weekly monitoring of eggs per gram of faeces (epg) values and faecal cultures from these animals provided data for the contamination patterns and the relative nematode population composition. At the same time, faecal pats were shaped and deposited monthly onto herbage and sampled weekly to determine the development time from egg to L3. Herbage samples were collected fortnightly over a 16-month period after deposition to evaluate the pasture larval infectivity and survival of L3 over time. The development time from egg to L3 was 1-2 weeks in summer, 3-5 weeks in autumn, 4-6 weeks in winter, and 1-4 weeks in spring. The levels of contamination and pasture infectivity showed a clear seasonality during autumn-winter and spring, whilst a high mortality of larvae on pasture occurred in summer. Ostertagia spp., Cooperia spp. and Trichostrongylus spp. were predominant and a survival of L3 on pasture over a 1-year period was recorded in this study.  相似文献   

14.
Pasture plots in 3 climatic regions were contaminated with worm eggs of Ostertagia ostertagi, Trichostrongylus axel, Haemonchus spp and Cooperia spp in the autumn, winter and spring. Successive pairs of parasite-free calves were grazed on the plots for 7 to 10 days at 4-week intervals and then killed for worm counts 14 days after their removal from pasture.
On the Northern Tablelands of New South Wales, irrespective of the season of pasture contamination, the degree of inhibition of O. ostertagi was low in winter and highest in spring. T. axei showed similar trends while Cooperia spp showed negligible inhibition.
On the North Coast of New South Wales, inhibited larvae accounted for a very small proportion of the O. ostertagi burdens, while in comparison T. axei showed a much greater degree of inhibition. Larval inhibition of Haemonchus spp occurred in autumn and early winter after which it did not occur. There was negligible inhibition in Cooperia spp.
On the Central Coast of New South Wales, there was little inhibition of O. ostertagi and none in T. axel . For Haemonchus spp, inhibited larvae were found mainly in autumn and winter. The numbers of inhibited Cooperia larvae were also highest in autumn and winter and were associated with large worm burdens.
The marked difference between the tablelands and coastal regions in the seasonal trends of inhibition of O. ostertagi was considered to be due to a difference in strains between the geographical regions. The possible effect of climatic factors on the inhibition-proneness of infective larvae on pasture is discussed for Ostertagia and other nematodes. The roles of host resistance and density-dependence are also discussed.  相似文献   

15.
A 3-year grazing trial (2002-2004) was conducted on a commercial beef cattle farm in south-central Sweden to assess different methods of parasite control. This paper focuses on the dynamics of the free-living larval stages, whereas data on performance and within-host parasitological variables are presented in a complementary paper. Each year in May, 4 groups of 10 first-season grazing (FSG) steers were turned out on to separate 2ha paddocks and subjected to the following strategies: (1) spring turn-out on to pasture which had been grazed the previous year by second-season grazing (SSG) steers (paddock RT), followed by a move to aftermath (paddock AM) after 10 weeks (mid-July), (2) supplementary feeding with concentrate and hay for 4 weeks following turn-out (paddock FD), set stocked, (3) untreated control (paddock UT), set stocked and (4) anthelmintic treated control (paddock DO), set stocked. All paddocks were assigned a new set of FSG cattle each year whereas the treatments remained the same. Pasture infectivity were monitored partly by two tracer calves that grazed each paddock along with the FSG calves for 3 weeks after turn-out and prior to housing, partly by analysis of herbage samples for infective larvae (L3) that were collected from each paddock at monthly intervals between April and October. The predominant genera found were Cooperia and Ostertagia. Tracers grazing paddock RT overall harboured less worms, and in particular less Ostertagia spp., and tracers grazing paddock AM in mid-July harboured insignificant numbers of nematodes compared to tracers on the FD and UT paddocks. Although total worm counts varied between groups, smaller numbers were generally observed early in the grazing-season (May), compared to close to housing (September) when inhibited early L4 larvae were almost exclusively found. Results observed from herbage samples showed high numbers of L3 in spring before the time of turn-out, compared to around housing. In conclusion, the rotation control strategy showed promising results and provided a turn-out pasture that was 'nematode safe' to FSG cattle the following spring, whereas the feeding strategy failed as applied in this experiment.  相似文献   

16.
Two groups of three month old, parasite-free calves grazed a permanently infected pasture for 14 days, Group A during the first two weeks of September and Group B during early November. Half of each group was killed 14 days after removal from the pasture and the remainder stabled overwinter before slaughter and parasitological examination. Marked inhibition of development occurred for Cooperia oncophora with a variable lower level of inhibition for Ostertagia ostertagi and practically none for Nematodirus helvetianus in those calves grazing late in the fall. Under the conditions of this study, inhibited Cooperia larvae resumed development in several calves soon after they were stabled while small numbers of Ostertagia resumed development regularly during the winter and spring with a considerable number of Ostertagia still present when the calves were slaughtered at the end of the stabling period. On the other hand, Nematodirus and practically all Cooperia worms were lost during the stabling period. In three of seven calves grazing late fall pastures, large Cooperia infections were either not established or failed to become patent.  相似文献   

17.
On the basis of the hypothesis that the peak numbers of infective nematode third-stage larvae (L3) on herbage in winter months results from fall contamination of pastures, 2 methods to reduce fall contamination were tested. In trial 1, morantal sustained-release boluses were administered to 15 fall-calving cows on Sept 7, 1982. Fifteen untreated cows (controls) were placed on separate pastures. Numbers of L3 on herbage during the winter and spring were assessed by use of worm-free tracer calves. In trial 2, 19 cattle due to calve in the fall were administered 200 micrograms of invermectin/kg of body weight, SC, on Sept 2, 1983. Also, 17 cattle similarly were given a placebo injection and served as control animals. Treated cattle were placed on the pasture used by control cattle in trial 1 and control cattle on the pasture used by treated cattle in trial 1. Worm-free tracer calves were again used to assess numbers of L3 on herbage. In trial 1, tracer calves grazing the control animal pasture from January 14 to 28 acquired 37 times as many nematodes as did those grazing the treated animal pasture. In trial 2, the greatest difference observed was a 10-fold increase of nematodes in calves grazing control animal pastures, compared with worm numbers in tracer calves grazing the treated animal pasture.  相似文献   

18.
AIM: To investigate the efficacy of pour-on anthelmintics against field strains of parasitic nematodes in young cattle on five farms in New Zealand. METHODS: Faecal nematode egg count (FEC) reduction (FECR) tests were carried out on five calf-rearing farms using pour-on formulations of levamisole, ivermectin, eprinomectin, and the simultaneous administration of levamisole and ivermectin. Faecal samples were collected per rectum before treatment and about 7, 14, 21 and 28 days after treatment, for FEC and faecal nematode larval culture. RESULTS: Resistance (i.e. <95% reduction in FEC) of Cooperia oncophora to ivermectin and eprinomectin was identified on all five farms. There was limited evidence of possible emerging resistance in Ostertagia spp to ivermectin but not eprinomectin, in short-tailed larvae of Cooperia spp to ivermectin and eprinomectin, and in Trichostrongylus spp to ivermectin, eprinomectin and levamisole used separately. Levamisole was effective against C. oncophora, but had variable efficacy against Ostertagia spp in the calves in this study. Simultaneous treatment with levamisole and ivermectin pour-on formulations were effective against all genera on all farms. CONCLUSIONS: To effectively manage roundworm parasites in their calves farmers need to be aware of the resistance status of the parasites on their farms. Levamisole is likely to be an effective anthelmintic on most farms at times of the year when the impact of Ostertagia spp is not high. Simultaneous administration of levamisole and ivermectin pour-on anthelmintics to cattle is likely to control both ML-resistant C. oncophora and stages of Ostertagia spp that are not controlled by levamisole alone.  相似文献   

19.
Field experiments were carried out for three consecutive years to study the influence of gastrointestinal nematodes in young cattle during their first grazing season. Free-living stages of Ostertagia ostertagi and Cooperia oncophora were found to survive the winter in the pasture in sufficient numbers to cause reduced weight gain and clinical disease, including deaths, in the calves. The symptoms were most pronounced during the first half of the grazing season.The mean weight gain of calves on parasite-free pastures was 48.3, 15.5 and 31.2 kg above those on contaminated pastures during the 3 years.Calves grazing contaminated pasture from the spring onwards, treated with thiabendazole and moved to aftermath in July, showed significantly better weight gain during the last part of the grazing season than calves which remained on the same contaminated pasture. It was, however, suggested that the better grazing conditions were the main cause of the improved weight gain on aftermath.  相似文献   

20.
A field study was undertaken of the possible differences in propensity for inhibited development in strains of cattle nematodes from two different locations in The Netherlands. In one location (Lelystad) the strains were thought to lack the ability for inhibited development as a result of environmental stimuli on the infective larvae, while in the other location (Utrecht) this ability was presumed to be present. The Lelystad strains were kept at the original location and were also transferred to a pasture in Utrecht, while the Utrecht strains remained in Utrecht. At both locations all permanent grazing calves showed high proportions of inhibited early fourth stage larvae of Ostertagia ostertagi, the proportion of the Utrecht strain being higher than the local and the transferred Lelystad strain. For Cooperia oncophora and Nematodirus helvetianus no clear differences between locations were observed in the permanently grazed calves. No inhibition occurred in tracer calves turned out in August in either location, but differences were seen among tracer calves turned out in October. The Lelystad strains, kept in Lelystad or transferred to Utrecht, showed no inhibition, while in the tracer calves grazed on pastures contaminated by the Utrecht strains a marked inhibition of O. ostertagi and N. helvetianus was observed. The Lelystad strains appear to lack the ability, possessed by the Utrecht strains, to inhibit their development in autumn in response to environmental stimuli.  相似文献   

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