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1.
J P Sundberg D L Hill E S Williams S W Nielsen 《American journal of veterinary research》1985,46(10):2200-2206
Cutaneous fibromas of white-tailed deer (Odocoileus virginianus), when compared with normal skin of the same species, had a thinner basement membrane; thickened stratum spinosum with numerous melanocytes, desmosomes, polyribosomes, and tonofilaments; focal hyperplasia of the stratum granulosum containing numerous large, electron-dense keratohyalin granules with irregular borders and containing occasional cells with diffuse intranuclear virus particles; and a moderately thickened stratum corneum with (although rarely) small crystalline arrays of virus particles. Normal mule deer (Odocoileus hemionus) skin was structurally similar to that of the white-tailed deer. Mule deer fibromas were similar to those in white-tailed deer, except for diffuse thickening of the stratum granulosum (the cells of which contained large keratohyalin granules of various electron densities with occasional composite granules) and except for a markedly thickened stratum corneum that contained numerous intranuclear viral inclusions. In negatively stained homogenates of tumors from both deer species, viral particles resembled papillomaviruses. 相似文献
2.
Chicken embryos and healthy adult chickens naturally infected with lymphoid leukosis virus were used to investigate viral inclusion bodies in myocardial cells by light and electron microscopies and by immunocytochemical technique. Intracytoplasmic viral matrix inclusion bodies frequently appeared in the myocardium of adult chickens, but not in that of embryos. In light microscopic preparations, inclusions were irregularly distributed, were basophilic, and contained ribonucleic acid. Ultrastructurally, inclusions in myocardial cells were in areas containing numerous interstitial C-type particles. Early inclusions were composed of clusters of ribosomes associated with sarcoplasmic tubules; spherical bodies developed among these ribosomes. Mature inclusions were composed of numerous spherical bodies (50 to 75 nm) with interspersed ribosomes and of ribosomes clustered at the periphery. Inclusions were not membrane-enclosed. Occasionally, spherical bodies were in paracrystalline arrays. Multiple budding occurred on cell membranes adjacent to matrix inclusions. The viral group-specific protein, p27, was demonstrated by the peroxidase-antiperoxidase method and by the protein A-gold method in the spherical bodies, in nucleoids of mature virus particles, and among ribosomes of inclusions. The results indicate that the matrix inclusions were the result of lymphoid leukosis virus infection and were the product of viral protein synthesis on ribosomes. 相似文献
3.
K S Latimer P M Rakich W L Steffens I M Kircher B W Ritchie F D Niagro P D Lukert 《Veterinary pathology》1991,28(4):300-304
The nature of feather inclusions was characterized in 32 psittacine birds (30 cockatoos, one peach-faced lovebird (Agapornis roseicollis), and one red-lored Amazon parrot (Amazona autumnalis autumnalis] with naturally-acquired psittacine beak and feather disease. Intranuclear inclusions within feather epithelial cells and intracytoplasmic inclusions within macrophages in the feather epithelium and pulp cavity contained psittacine beak and feather disease viral antigen when stained by the avidin-biotin complex immunoperoxidase technique. Ultrastructurally, inclusions were observed primarily within macrophages and to a lesser extent within epithelial cell nuclei. Macrophage inclusions appeared as paracrystalline arrays of viral particles. Intranuclear inclusions were less well defined, although scattered viral particles were present. Intracytoplasmic and intranuclear particles in ultrastructural preparations were identified by colloidal gold labeling as psittacine beak and feather disease virus. Feather epithelium was more frequently and severely involved in the disease process than was adjacent follicular epithelium. Plucked feathers with an intact epidermal collar and feather epithelium were preferred to follicular biopsies for histopathologic examination. 相似文献
4.
?ystein Wessel Finstad Maria Krudtaa Dahle Tone H?g Lindholm Ingvild Berg Nyman Marie L?voll Christian Wallace Christel Mor?us Olsen Anne K Storset Espen Rimstad 《Veterinary research》2014,45(1):35
Piscine orthoreovirus (PRV) belongs to the Reoviridae family and is the only known fish virus related to the Orthoreovirus genus. The virus is the causative agent of heart and skeletal muscle inflammation (HSMI), an emerging disease in farmed Atlantic salmon (Salmo salar L.). PRV is ubiquitous in farmed Atlantic salmon and high loads of PRV in the heart are consistent findings in HSMI. The mechanism by which PRV infection causes disease remains largely unknown. In this study we investigated the presence of PRV in blood and erythrocytes using an experimental cohabitation challenge model. We found that in the early phases of infection, the PRV loads in blood were significantly higher than in any other organ. Most virus was found in the erythrocyte fraction, and in individual fish more than 50% of erythrocytes were PRV-positive, as determined by flow cytometry. PRV was condensed into large cytoplasmic inclusions resembling viral factories, as demonstrated by immunofluorescence and confocal microscopy. By electron microscopy we showed that these inclusions contained reovirus-like particles. The PRV particles and inclusions also had a striking resemblance to previously reported viral inclusions described as Erythrocytic inclusion body syndrome (EIBS). We conclude that the erythrocyte is a major target cell for PRV infection. These findings provide new information about HSMI pathogenesis, and show that PRV is an important factor of viral erythrocytic inclusions. 相似文献
5.
6.
A female bearded dragon (Amphibolurus barbatus) died following intermittent periods of inappetance. No significant gross lesions were found at autopsy, but histological examination revealed disordered liver architecture with numerous foci of coagulative necrosis. Eosinophilic intranuclear inclusions were present in many hepatocytes, some epithelial cells of the bile ductules and occasional epithelial cells of renal tubes and glomeruli. Large numbers of viral particles within many nuclei, associated with the intranuclear inclusion were demonstrated by electronmicroscopy. Similar particles, sometimes in paracrystalline arrays, were also seen within membrane-bound vesicles located next to the nuclei and to a lesser degree within the cytoplasm and extracellular spaces. The virus was considered to be an adenovirus on the basis of its size, morphology, site of formation and lack of envelopment. It was considered to he the cause of the hepatitis. 相似文献
7.
A female bearded dragon (Atnphibolurus barbutus) died following intermittent periods of inappetance. No significant gross lesions were found at autopsy, but histological examination revealed disordered liver architecture with numerous foci of coagulative necrosis. Eosinophilic intranuclear inclusions were present in many hepatocytes, some epithelial cells of the bile ductules and occasional epithelial cells of renal tubes and glomeruli. Large numbers of viral particles within many nuclei, associated with the intranuclear inclusion were demonstrated by electronmicroscopy. Similar particles, sometimes in paracrystalline arrays, were also seen within membrane-bound vesicles located next to the nuclei and to a lesser degree within the cytoplasm and extracellular spaces. The virus was considered to be an adenovirus on the basis of its size, morphology, site of formation and lack of envelopment. It was considered to he tlie cause of the hepatitis. 相似文献
8.
C Olson R O Olson S Hubbard-Van Stelle 《Journal of the American Veterinary Medical Association》1992,201(1):56-62
The common bovine papilloma virus type 1 has been widely used to stimulate basic research on papilloma viruses involved in some cancers of mankind. The usually benign neoplasms of cattle caused by bovine papilloma viruses are frequent clinical problems for veterinarians. Approximately 240 experimentally induced cutaneous fibropapillomas on 8 susceptible calves had a uniform appearance of initial growth. Their size and duration ranged from about 2 months to nearly 3 years but were similar for the multiple papillomas of each calf. Sequential biopsies were done to examine the histologic changes and existence of viral antigen. Veterinarians in practice may encounter the common fibropapillomas caused by bovine papilloma virus 1 and 2 as well as papillomas with no fibromatous element. These types may develop on teats of cows in some herds. Interdigital papillomas cause a problem in some dairy herds and a virus suspected but not yet found. Prophylactic vaccination with a formalin-killed vaccine will protect against infection with bovine papilloma virus 1 and 2. 相似文献
9.
A 12-year-old domestic Shorthaired cat developed a multinodular exophytic mass on the dorsal surface of the nose. The skin surrounding the mass was nonpigmented, and actinic keratosis had been diagnosed in this area 3 years previously. Histologic examination revealed hyperkeratosis, epidermal hyperplasia, papillomatosis, koilocytosis, and possible intranuclear viral inclusions. Polymerase chain reaction amplified papillomaviral deoxyribonucleic acid from formalin-fixed samples of the lesion. Sequencing of the amplicon revealed 98% similarity to human papillomavirus (HPV) type 9. To the authors' knowledge, this is only the second reported feline cutaneous viral papilloma. In addition, this is the first report of a feline papilloma being associated with an HPV. 相似文献
10.
Out of a sample of 200 rumens from sheep slaughtered in Edinburgh, papillomas were found in 25. They occurred as fibro-papillomas, mostly along the pillar of muscle between the dorsal and ventral rumen, and were often multiple. No inclusion bodies were seen in the lesions or papilloma virus particles on electron microscopy. Homogenisation of papillomatous tissue followed by various methods of purification did not yield identifiable virus particles, and viral DNA was not detected. Immunoperoxidase staining showed a very small number of positive cells at or on the surface of 6 out of 10 lesions examined. Thus, it seems probable that virus particles are not found in large numbers in the rumen papillomas of sheep, unlike the situation in ovine skin warts, but are present in a few epithelial cells which are rapidly shed from the surface of the mucous membrane. 相似文献
11.
J H Park H Kida K Ueda K Ochiai M Goryo C Itakura 《Zentralblatt für Veterin?rmedizin. Reihe B. Journal of veterinary medicine. Series B》1991,38(10):749-754
Causative agent of rabbit haemorrhagic disease (RHD) was purified by CsCl density gradient centrifugation from the liver homogenate of rabbits infected with RHD virus which originated from Korea. The viral particles were 35-40 nm in diameter, and had hollow depressions on their surface. Protein A-gold immunoelectron microscopy clearly showed that the convalescent antisera of diseased rabbits reacted specifically with the virus particles. SDS-PAGE and Western blot analyses demonstrated that the structural protein of the virus was composed of a single major polypeptide of 63 kD. These findings indicate that the causative agent of RHD, tentatively named as picornavirus in Korea, belongs to calicivirus. 相似文献
12.
Nakamura K Mase M Yamamoto Y Takizawa K Kabeya M Wakuda T Matsuda M Chikuba T Yamamoto Y Ohyama T Takahashi K Sato N Akiyama N Honma H Imai K 《Avian diseases》2011,55(4):719-723
From January 2009 to June 2010, many broiler chicks suddenly died without clinical signs. The mortality rates were from 1.2% to 17.0% in affected flocks. Inclusion body hepatitis (IBH) was detected in 13 prefectures (northern, eastern, western, and southern areas) in Japan. The livers were enlarged and pale. The bursa of Fabricius and thymus had not atrophied. Multifocal necroses of hepatocytes with basophilic intranuclear inclusions were seen in the liver. Eosinophilic intranuclear inclusion bodies in hepatocytes were rare. Focal necrosis of acinar cells with basophilic intranuclear inclusions was found in the pancreas. Basophilic intranuclear inclusion bodies were detected in intact surface epithelial cells of gizzard and epithelial cells of the small intestine. The intranuclear inclusions of liver, pancreas, gizzard, and small intestine were stained positively for immunohistochemistry of fowl adenovirus (FAV) antigen. Ultrastructurally, basophilic intranuclear inclusions consisted of viral particles approximately 70 nm in diameter and arranged in a crystalline array. FAV was isolated from the liver of chickens affected with IBH. The serotype of most isolates was 2. This study suggests that IBH produced by FAV is epidemic in broiler chicks in Japan and that the present cases occurred as the primary disease without the association of infectious bursal disease virus or chicken anemia virus. 相似文献
13.
Seven 19-day-old broiler chickens affected with hydropericardium syndrome (HPS) with pancreatic necrosis and gizzard erosions were investigated pathologically and virologically. Mortality increased after 13 days of age in a flock on a broiler farm. The mortality rate of the flock reached 10% by 19 days of age. Macroscopically, the chickens had hydropericardium (the characteristic gross change of HPS), pinpoint white foci in the pancreas, and ventricular erosions. Histologically, the chickens had multifocal hepatic necrosis with intranuclear inclusions in hepatocytes, a marked increase of macrophages in the spleen and lung, mild epicardial edema, multifocal necrosis of pancreatic acinar cells with intranuclear inclusions, focal necrosis of the ventricular koilin layer, and degeneration of the ventricular glandular epithelium with intranuclear inclusions. Immunohistochemically, intranuclear inclusions in the liver, pancreas, and ventriculi were stained positively against group I avian adenovirus (GIAAV) antigens. Ultrastructurally, 67-nm diameter viral particles were present in intranuclear inclusions. Virologically, serotype 4 of GIAAV was isolated from the liver, heart, and kidney of affected chickens. The pathologic changes of the present cases differ from previous cases of HPS; therefore, the present strain of GIAAV may have different pathogenicity for chickens than the previous virus strain of HPS. 相似文献
14.
Y Yoshikawa F Ochikubo Y Matsubara H Tsuruoka M Ishii K Shirota Y Nomura M Sugiyama K Yamanouchi 《Veterinary microbiology》1989,20(3):193-205
A case of encephalitis in a Japanese monkey (Macaca fuscata) was examined histopathologically and serologically. The animal had brain lesions consisting of perivascular cuffs, malacia, inclusion bodies and giant cells. Monoclonal antibody to the nucleoprotein of canine distemper virus (CDV) stained the inclusions, and the distribution of the virus antigen was closely associated with that of the histological lesions. Serologically, all the 22 monkeys in the same group as the diseased monkey had relatively high titers of neutralizing antibody to CDV, but not to measles virus (MV). The pattern of the antibody titers to CDV and MV closely resembled that of cynomolgus monkeys experimentally inoculated with CDV, but differed from that of monkeys inoculated with MV. These findings suggest that an epidemic of CDV occurred in these Japanese monkeys, associated with one case of fatal viral encephalitis. This is believed to be the first report of a natural infection by CDV in non-human primates. 相似文献
15.
C J O'Loan W L Curran M S McNulty 《Zentralblatt für Veterin?rmedizin. Reihe B. Journal of veterinary medicine. Series B》1992,39(6):459-466
The morphology and morphogenesis of five viruses isolated in Great Britain, France and South Africa from turkeys with rhinotracheitis were examined. The five isolates were antigenically related by immunofluorescence and were indistinguishable by negative contrast and thin section electron microscopy. Negative contrast electron microscopy of infected Vero cell cultures revealed ortho- or paramyxovirus-like particles and helical nucleocapsids 14 nm in diameter with a pitch of 6 nm. The viral nature of these structures was confirmed by immuno-gold labelling, using a hyperimmune rabbit antiserum to TRT virus and 15 nm gold-labelled goat anti-rabbit IgG. Ultrastructural changes characteristics of paramyxovirus infection were observed in Vero cell cultures infected with each of the five TRT virus isolates. These alterations, which included areas of localised thickening of plasma membrane, associated cytoplasmic inclusions of nucleocapsids and budding virus particles also labelled specifically following immunogold staining. These observations are in accord with the suggestion that TRT virus is an avian pneumovirus. 相似文献
16.
Catherine M Shilton Dale A Smith Leslie W Woods Graham J Crawshaw Howard D Lehmkuhl 《Journal of zoo and wildlife medicine》2002,33(1):73-79
Adenoviral infection was associated with hemorrhagic enteritis, serosal hemorrhages, and severe pulmonary edema in six captive moose (Alces alces) in Toronto, Ontario, Canada: an adult female moose and three calves in 1985 and two calves in 1998. Adenoviral disease was suspected based on histological findings of systemic vasculitis and widespread thrombosis associated with amphophilic intranuclear inclusions in endothelial cells. Diagnosis was confirmed by immunohistochemistry using antiserum to bovine adenovirus type 5, transmission electron microscopic identification of viral particles consistent in morphology with adenovirus within nuclei of pulmonary endothelial cells in an affected calf, and virus isolation. The restriction pattern of virus isolated from the lung of one of the calves indicated that the virus was identical to a recently characterized adenovirus in black-tailed deer (Odocoileus hemionus) in California. The moose adenovirus reported here may have been endemic in the captive moose herd, or infection may have resulted from either direct or indirect contact with other species of captive or wild cervids. This is the first report of adenoviral infection in moose and of the presence of adenoviral disease in a cervid in Canada. 相似文献
17.
Kiatipattanasakul-Banlunara W Tantileartcharoen R Katayama K Suzuki K Lekdumrogsak T Nakayama H Doi K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(6):527-529
Three sulphur-crested cockatoos (Cacatua galerita) were diagnosed as psittacine beak and feather disease (PBFD). Histopathology of the feather pulp and follicles showed intracytoplasmic botryoid clusters or granular inclusion bodies in epithelial cells and macrophages. Electron microscopy revealed multiple cytoplasmic clusters of electron dense viral particles corresponding to the inclusions. PBFD virus (circovirus) DNA-specific product was detected from formalin-fixed paraffin-embedded feathers by nested polymerase chain reaction (PCR) method. 相似文献
18.
Strain A/swine/Wisconsin/1/68 (WI/68) swine influenza virus (SIV) was propagated in embryonating chicken eggs at 33, 35, or 37 C. The SIV harvested from eggs incubated at 33 C invariably had higher hemagglutination (HA) and egg infectivity titers than did SIV propagated in eggs at the 2 higher temperatures. When SIV inoculum propagated at 33 C was inoculated into separate groups of eggs and incubated at 33, 35, and 37 C, the SIV harvested from inoculum incubated at the 2 higher temperatures had significantly lower infectivity and HA titers than did that propagated at 33 C. By electron microscopy (EM), viral particles of Wi/68 were of various sizes and shapes regardless of the temperature used to propagate the virus. However, in contrast to what was seen in SIV harvested from innoculum incubated at 33 C incubation, pleomorphic shapes and particles with surface abnormalities were much more frequent in SIV harvested from inoculums kept at the 2 higher temperatures. Approximately one-third of the particles from 35 and 37 C incubation either were spikeless or were relatively deficient in surface spikes. 相似文献
19.
McLaughlin BG Adams PS Cornell WD Elkins AD 《The Canadian veterinary journal. La revue veterinaire canadienne》1985,26(12):368-372
Four cases of canine distemper were detected by the presence of numerous cytoplasmic inclusions in various circulating blood cells. Fluorescent antibody techniques and electron microscopy confirmed the identity of the viral inclusions. The cases occurred in the same geographic area and within a short time span. All four dogs had been vaccinated against canine distemper, but stress or other factors may have compromised their immune status. The possibility of an unusually virulent virus strain was also considered. 相似文献