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Forest restoration projects with Holm oak (Quercus ilex) have had limited success, mostly due to water stress after planting and poor plant quality. Recent studies indicated that large and nutrient rich plants perform better in Mediterranean areas, suggesting that late-season fertilization may improve plant quality and field performance. The purpose of this study was to assess the effect of late-season fertilization on the quality of Holm oak seedlings, as determined by morphological, nutritional, and water relations analyses. We grew Holm oak nursery seedlings under 5 different late-season fertilization regimes and then analyzed morphological characteristics, nutritional status, and water relations parameters of the fertilization groups at the end of the nursery period. We also analyzed the effect of fertilization on nutritional status by use of vector nomograms. Our results indicated that late-season NPK fertilization improved shoot and root growth, and the overall nutritional status of seedlings. The lack of late-season fertilization leads to nutrient deficiency in plants, whilst the application of imbalanced fertilization treatments may trigger nutrient luxury consumption and nutrient dilution, pointing out the importance of NPK proportions in the fertilizer. Moreover, late-season fertilization with nitrogen might improve the drought resistance of seedling by enhancing their osmotic adjustment. 相似文献
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To assess the effects of seedling spacing on one-year-old seedling morphology in the nursery, seeds of three provenances of Fraxinus angustifolia were sown at five different seedling spacings within rows of two different spacings in the seedbed. Subsequent growth performance of one-year-old seedlings was assessed by planting in the forest. Within row spacings were: 4.3, 5.0, 6.3, 8.3, and 12.5 cm, and there were five rows at 20 cm apart, or three rows at 33 cm apart across the 1.2 m wide seedbeds. Both spacings within and between rows significantly affected shoot height, root collar diameter, root dry weight and shoot dry weight, but not root/shoot ratio. Wider spacings produced larger seedlings, but only the wider spacing within rows significantly increased fine and coarse root mass. Provenances showed significant differences in diameter, root/shoot ratio, and fine and coarse roots, and they also showed interactions with row spacings in height and diameter measurements. One year after outplanting, diameter growth was significantly related to provenance, and diameter growth was 88% greater for trees from 33 cm nursery row spacing than those from 20 cm nursery row spacing. 相似文献
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Free amino acids and protein in Scots pine seedlings cultivated at different nutrient availabilities
Scots pine (Pinus sylvestris L.) seedlings of a provenance from northern Sweden were cultivated hydroponically for 7 weeks in a climate chamber. The nutrient solution contained either 2.5 (low-N) or 50 (high-N) mg N l(-1) with other essential elements added in a fixed optimal proportion to the nitrogen. After 5 and 7 weeks, the seedlings were analyzed for growth, total nitrogen and other essential nutrients, protein and free amino acids. Low-N seedlings grew more slowly and had higher root/shoot ratios than high-N seedlings. With respect to total nitrogen, the effect of the lower nutrient supply was mainly on the nitrogen content of the whole plant and the allocation of nitrogen among tissues, not on tissue nitrogen concentration. This was also the case for potassium, phosphorus, calcium and magnesium. The proportions by weight among these macronutrients in the whole seedlings were similar in both nutrient regimes. The proportion and concentration of sulfur were significantly lower in low-N seedlings than in high-N seedlings, because of a lower net uptake of sulfur than of other macronutrients. The shoot, needles and stem of low-N seedlings had higher concentrations of free amino acids and lower concentrations of protein than the shoot, needles and stem of high-N seedlings. Arginine dominated the pool of free amino acids in the low-N seedlings, whereas glutamine predominated in the high-N seedlings. We conclude that Scots pine seedlings accumulated soluble nitrogen as arginine when net protein synthesis was limited by factors other than nitrogen availability. Nutritional imbalance, as revealed by growth characteristics and a suboptimal proportion and concentration of sulfur in the seedlings, probably affected synthesis of S-amino acids, resulting in the diversion of assimilated nitrogen to arginine instead of protein. 相似文献
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Fertilization is essential to seedling production in nursery culture, but excessive fertilization can contaminate surface
and ground water around the nursery. The optimal fertilization practice is that which maximizes seedling growth and minimizes
nutrient loss. We tested three fertilization strategies: (1) constant fertilization (2) a three-stage rate, and (3) exponential
fertilization on Liriodendron tulipifera and Larix leptolepis containerized seedlings. Growth performance, nutrient uptake, and nutrient loss in leaching were measured. Height, root collar
diameter, and dry weight of both species were not significantly different among treatments even though the nutrient supply
of the exponential treatment was half that of the constant and three-stage treatments. Generally, nutrient losses in leached
solutions were higher in constant and three-stage than the exponential treatment. Nutrient use efficiency was calculated as
the ratio of the nutrient content of the seedlings to the amount of nutrient applied to the containers. The nitrogen use efficiency
in the constant, three-stage, and exponential treatments was 63, 61, and 85% for yellow poplar, respectively, and 35, 30,
and 53% for larch. Similar results were obtained for phosphorus and potassium. Thus, the exponential treatment had the highest
nutrient use efficiency as well as the least nutrient loss. Adjusting fertilization rates can reduce soil and water contamination
around the nursery without compromising growth performance, which reduces both producer’s investments and environmental impacts. 相似文献
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Carbon assimilation by Cedrela odorata L. (Meliaceae) seedlings was investigated in ambient and elevated CO2 concentrations ([CO2]) for 119 days, using small fumigation chambers. A solution containing macro- and micronutrients was supplied at two rates. The 5% rate (high rate) was designed to avoid nutrient limitation and allow a maximum rate of growth. The 1% rate (low rate) allowed examination of the effect of the nutrient limitation-elevated CO2 interaction on carbon assimilation. Root growth was stimulated by 23% in elevated [CO2] at a high rate of nutrient supply, but this did not lead to a change in the root:shoot ratio. Total biomass did not change at either rate of nutrient supply, despite an increase in relative growth rate at the low nutrient supply rate. Net assimilation rates and relative growth rates were stimulated by the high rate of nutrient addition, irrespective of [CO2]. We used a biochemical model of photosynthesis to investigate assimilation at the leaf level. Maximum rate of electron transport (Jmax) and maximum velocity of carboxylation (Vcmax) did not differ significantly with CO2 treatment, but showed a substantial reduction at the low rate of nutrient supply. Across both CO2 treatments, mean Jmax for seedlings grown at a high rate of nutrient supply was 75 micromol m(-2) s(-1) and mean Vcmax was 27 micromol m(-2) s(-1). The corresponding mean values for seedlings grown at a low rate of nutrient supply were 36 micromol m(-2) s(-1) and 15 micromol m(-2) s(-1), respectively. Concentrations of leaf nitrogen, on a mass basis, were significantly decreased by the low nutrient supply rate, in proportion to the observed decrease in photosynthetic parameters. Chlorophyll and carbohydrate concentrations of leaves were unaffected by growth [CO2]. Because there was no net increase in growth in response to elevated [CO2], despite increased assimilation of carbon at the leaf level, we hypothesize that the rate of respiration of non-photosynthetic organs was increased. 相似文献
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Juan A. Oliet María Tejada K. Francis Salifu Aleyda Collazos Douglass F. Jacobs 《European Journal of Forest Research》2009,128(3):253-263
Holm oak (Quercus ilex L.) seedlings were exponentially (E) nutrient loaded using incremental increases in fertilizer addition or conventionally
(C) fertilized using a constant fertilizer rate during nursery culture. The fertility treatments (mg N plant−1) were control (0), 25E, 100E, and 100C. Subsequently, 1-year-old plants were transplanted under simulated soil fertility
gradients in a greenhouse to evaluate effects of nutrient loading and post-transplant fertility on seedling performance. Post-transplant
fertility consisted of fertilizing plants at two rates (0 vs. 200 mg N plant−1). A water-soluble fertilizer 20-20-20 was supplied in both nursery and post-transplant experiments. Nutrient loading increased
plant N content by 73% in 100E and by 75% in 100C relative to controls, although no significant differences were detected
between constant and exponential fertilization regimes at the 100 mg N plant−1 rate. When transplanted, nutrient loading promoted post-transplant root growth relative to shoot, implicating potential to
confer competitive advantage to loaded holm oak seedlings after trans-planting. In contrast, post-transplant fertility increased
new shoot dry mass by 140% as well as N, P and K content relative to controls. Results suggest that holm oak seedlings can
be successfully nutrient loaded in the nursery at higher fertility rates, improving its potential to extend new roots, but
alternative fertilization regimes and schedules that better fit nutrient availability to the growth rhythm and conservative
strategy of this species must be tested. 相似文献
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The effect of two arbuscular mycorrhizal (AM) fungi, Glomus fasciculatum and G. macrocarpum on shoot and root dry weights and nutrient content of Cassia siamea in a semi-arid wasteland soil was evaluated. Under nursery conditions, mycorrhizal inoculation improved growth of seedlings. Root and shoot dry weights were higher in mycorrhizal than non-mycorrhizal plants. The concentration of P, K, Cu, Zn and Na was significantly higher in AM inoculated seedlings than in non-inoculated seedlings. Mycorrhization led to decrease in alkalinity of the rhizosphere soil from pH 8.5 to 7.4. Under nursery conditions, the degree of mycorrhizal dependency increased with age of C. siamea seedling. On transplantation to the field, the survival rate of mycorrhizal seedlings (75%–90%) was higher than that of non-mycorrhizal seedlings (40%). Besides better survival rate, AM inoculation improved the growth performance of seedlings in terms of height and stem diameter. Among the two AM fungi used, the efficiency of Glomus macrocarpum was higher than that of G. fasciculatum under both nursery and field conditions.This revised version was published online in May 2005 with corrected page numbers. 相似文献
9.
杉木营养平衡与苗木干物质的分配关系 总被引：6，自引：3，他引：6
详细研究了不同营养 (N、P、NP)条件下杉木苗木干物质在根冠的分配规律 ,结果表明 ,不同的营养条件可导致苗木光合产物在根冠发生明显再分配 ,苗木的营养状态和根冠干物质分配的关系规律可应用异速生长模型y =axk 来描述和刻划。基于营养与光合产物在根冠的再分配规律及异速生长模型参数k值对营养胁迫反应的敏感性变化规律 ,提出了新的营养平衡理论和营养诊断方法 (称之为苗木群体营养诊断法 )。营养诊断法则为 :(1)在某一营养条件下 ,如果苗木异速生长模型参数k <1时 ,表明根系相对生长速率高于冠层相对生长速率 ,苗木处于营养亏缺状态 ;(2 )当k >1时 ,表明苗木冠层相对生长速率高于根系 ,苗木处于营养过剩状态 ,一般在自然条件下 ,k >1是不会出现的 ;(3)当k =1时 ,表明根系和冠层相对生长速率相等 ,苗木处于营养平衡状态。根据新的营养诊断方法 ,制定了杉木叶片和土壤营养诊断标准 ,并通过大量的杉木幼、中、近熟林施肥试验结果验证了营养平衡理论的正确性。 相似文献
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Early establishment of seedlings in reclaimed oil sand areas is often limited by low nutrient and water availability due to factors such as strong understory vegetation competition. Management practices such as nursery fertilization and field weed control could help early establishment of planted seedlings and reclamation success. We investigated the effect of nursery nutrient loading and field weed control on the growth, nitrogen (N) retranslocation within seedling components, and plant N uptake from the soil for white spruce (Picea glauca [Moench] Voss) seedlings planted on a highly competitive reclaimed oil sands site for two years. Exponential fertilization during nursery production increased the root biomass but not the nutrient reserve in the seedling. In the field experiment, on average across the treatments, 78 and 49% of the total N demand of new tissue growth in the first and second year were met by N retranslocation, respectively. Though exponential fertilization did not affect N retranslocation, it increased the percent height and root collar diameter growth. Weed control increased not only the growth of seedlings by increasing soil N availability, but also N retranslocation within the seedlings in the second year after outplanting. We conclude that vegetation management by weed control is feasible in improving the early growth of white spruce seedlings planted on reclaimed soils and facilitate tree establishment in the oil sands region. Optimization of the nursery exponential N fertilization regime for white spruce may further help with early revegetation of reclaimed oil sands sites. 相似文献
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Effects of nitrogen source on growth and activity of nitrogen-assimilating enzymes in Douglas-fir seedlings 总被引：2，自引：0，他引：2
Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) seedlings were grown for 68 days in a growth chamber in nutrient solutions with ammonium, nitrate or ammonium nitrate as the nitrogen source. Among the nitrogen sources tested, whole-seedling biomass, relative growth rate (RGR), root and shoot elongation, and number of lateral roots, were greatest in seedlings grown with ammonium. In the absence of nitrogen, plant growth and formation of lateral roots were poor. Initially, glutamine synthetase, NAD-glutamate dehydrogenase and aspartate aminotransferase activities were high in young roots and shoots, but all three enzymatic activities decreased after one month of culture. In root apices, glutamine synthetase and aspartate aminotransferase activities were higher than NAD-glutamate dehydrogenase activity. Enzymatic activities were often higher in ammonium-fed seedlings than in seedings supplied with the other forms of nitrogen. Activities of all three enzymes were significantly reduced in seedlings grown in the absence of nitrogen. The beneficial effect of ammonium is discussed on the basis of its involvement in the assimilation pathways of Douglas-fir. 相似文献
13.
B.M.Khan M.K.Hossain M.A.U.Mridha 《林业研究》2006,17(2):99-102
雨豆树的苗木种植在容器袋的沙和牛粪的混合质上(3:1)。在播种前后一周施用不同浓度(0.1%, 0.5%, 1%, 2%, 5% and 10%)的有效微生物群(Effective Microorganisms )液。测定发芽和苗木自然生长参数(包括:茎根长度,活力指数,直径,叶子数量,茎根的鲜重和干重,总的生物量增长量)。估计生化参数如叶绿素 a, 叶绿素 b,类胡罗卜素,并观测了受有效微生物群影响的生节情况。与对照相比, 经不同浓度有效微生物群液处理的雨豆树,发芽率和苗木自然生长参数都明显增加。施用 2%有效微生物群液的苗木生长率最大,其次是施用 1%有效微生物群液的苗木。1%有效微生物群液的生节率最高,并随浓度的增加而下降。尽管经有效微生物群液处理的苗木叶中色素比对照高,但叶绿素 a, 叶绿素 b,类胡罗卜素并不明显高于对照。随着有效微生物群液浓度的增加,苗木生长表现出不同的效果,大部分参数在中等浓度范围最高。研究表明,有效微生物群技术有助与提高苗圃中苗木的生长,有益微生物与容器袋土壤相结合有助于改善退化的或贫瘠的土壤,有利于初移植苗木的营养和水分吸收。表 4 参 17。 相似文献
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This research examined the first year growth characteristics of cold stored and transplanted nursery-produced aspen (Populus tremuloides) seedlings (container and bareroot (BR)) and compared it to the growth of seedlings that had not been transplanted (established
from germinants in the field) and therefore had an unrestricted root system (UR). Prior to planting, nursery-produced seedlings
were placed in cold storage (−3°C) and root growth potential (RGP) and total non-structural carbohydrate (TNC) root reserves
were tested at 0, 10, 75 and after 150 (container) and 190 days (BR) of storage. Both container and BR stock had much lower
root to shoot ratios (RSRs) and root carbohydrate reserves compared to UR seedlings after 170 days. During storage, root reserves
in container stock declined faster than in the BR and UR seedlings. RGP in all nursery stock was the highest after 75 days
of storage, while longer storage resulted in shoot dieback and reduced root growth. After the first growing season, UR seedlings
were one tenth the size of the nursery stock; however, in the second growing season they had no stem dieback and grew twice
the height and stem diameter. The higher RSRs and root reserves in the UR seedlings was likely caused by early bud set in
its first year of growth. This suggests that inducing bud set earlier in the growing regime might allow seedlings to increase
root mass and carbohydrate reserves. 相似文献
15.
通过进行酸枣嫁接金丝王大枣苗试验研究,结果表明,不同的嫁接方法、不同嫁接时期影响到嫁接苗的成活率,不同的施肥配方均影响到苗木新梢生长量;春季4月24日左右采用插皮接较之其他嫁接方法能显著提高嫁接苗成活率;采用氮、磷和硫基钾其三元素配比为2∶1∶1养分配比能明显提高新梢生长量,与其他配比有极显著差异,当年新梢生长达到了58.3cm。 相似文献
16.
Nutrient loading of nursery seedling stock of species with an indeterminate growth strategy is challenging and poorly understood. Here, we explore the use of two potential techniques for nutrient loading of trembling aspen (Populus tremuloides Michx.) seedlings: (1) exponential fertilization and (2) early shoot growth termination in order to divert assimilated nutrients and carbon to storage rather than to growth. In the first study, aspen seedlings were treated with either exponential or constant fertilization rates, both of which supplied the same amount of nutrients over the growing season. Exponential fertilization resulted in overall poor planting stock form (stunted seedling growth and weak root development) and produced only marginal improvements of nutrient status. As a result, the exponential fertilization regime studied cannot be recommended as a treatment for aspen seedlings. In the second study we treated seedlings with a 2 × 2 factorial combination of fertilization and shoot growth inhibitor (SGI) applications with the fertilizer treatments varying in terms of mid-season fertilizer concentrations. Seedlings with SGI application had much higher tissue nutrient and carbon reserve concentrations than seedlings without a SGI application. In addition, nutrient uptake appeared to be more efficient in SGI treated seedlings, which could potentially result in significant reductions of nutrient application rates during aspen seedling production in nurseries. Overall, early shoot growth termination using a SGI appears to be an effective technique to produce nutrient loaded aspen seedlings. 相似文献
17.
污泥对苗圃生长的银合欢幼苗发芽和初期长势的影响 总被引：1，自引：1，他引：0
研究了不同类型的污泥(城市的、工业的和住宅污泥)对苗圃生长的银合欢幼苗田间萌发、生长和分枝的影响.播种前先将不同类型污泥的混合物与养分匮乏的自然林土壤混合.播种的3和6月后,记录幼苗大田发芽、分枝状况和其他物理生长参数(枝条或根长、活力指数、茎直径、叶片数、分枝或根鲜重和干重、总的生物量干重增长)等.与对照幼苗相比,混合污泥的土壤中生长的幼苗田间发芽、分枝状况及其他生长参数均发生了显著变化.与其它条件生长的幼苗相比,住宅污泥与土壤混合(1:1)条件生长的3月龄和6月龄幼苗分枝数和分枝鲜或干重均最高.就生长参数而言,住宅污泥与自然林土壤混合(1:1)生长的幼苗长势最好.研究表明:退化的土壤补偿以住宅污泥可促进银合欢的田间发芽、生长以及分枝的形成.图1表3参29. 相似文献
18.
《Scandinavian Journal of Forest Research》2012,27(5):401-415
Norway spruce [Picea abies (L.) Karst.] and silver birch (Betula pendula Roth) seedlings were grown for one season under three different fertilization regimens in the forest nursery. During the first 50 days the seedlings were grown in a glasshouse, and thereafter outdoors until the beginning of September. Finally, the plants were exposed to 16 h nights in the glasshouse throughout September. When the seedlings were supplied with fertilizers at a rate adjusted to expected plant demand (RO), less than half as much of each nutrient was applied as in a conventional regimen (RC), in which equal amounts were supplied per unit time during the growth season, yet the plants still looked healthy. Utilization of N increased by almost 50% in spruce when supply was adjusted to plant demand. In the third treatment (RL), nutrients were supplied as in the RO treatment, but at a growth-limiting rate. These plants were loaded with nutrients at the end of the season and had higher root:plant ratios, i.e. root weight in relation to total plant weight, compared with the other treatments. The nutrient status of the plants was not growth limiting at the end of the growing season in any of the treatments. The plants given the different treatments differed in size at planting out, but they had similar heights after 3 yrs in the field. This indicates that the root:plant ratio may be important for growth performance, provided that nutrient status is not at a growth-limiting level. This study suggests that the use of fertilizers can be considerably reduced in Swedish forest nurseries. 相似文献
19.
《Scandinavian Journal of Forest Research》2012,27(5):409-414
Second-year Norway spruce seedlings [Picea abies (L.) Karst.] grown in containers were divided into three fertilization levels in August [5, 15 and 25 mg nitrogen (N) seedling?1]. The resulting foliar concentrations of N were 11.0, 13.1 and 15.8 g kg?1, respectively. Seedling height (mean 26.0 cm) did not differ among treatments. The next spring, the seedlings were tested in two experiments. (1) The seedlings were transplanted into pots containing sandy soil in the greenhouse, where they were fertilized with either pure water or nutrient solution (22 mg N l?1). (2) The outplanting performance of the autumn-fertilized seedlings was tested on a sandy field. In the greenhouse experiment, the autumn fertilization level affected height growth and root egress of the seedlings significantly, but less than fertilization with a nutrient solution after planting. In the field experiment, during the first season after transplanting shoot growth of the seedlings increased with the level of autumn fertilization. After the second and third seasons, the seedling stem volume was highest with the highest level of autumn fertilization. These results suggest that, by improving the preplanting nutrient status of seedlings, the growth of shoot, stem diameter and root biomass can be enhanced after planting, especially on nutrient-poor soils. However, heavier autumn fertilization than that used here may yield a greater and more persistent increment in growth. 相似文献
20.
Growth, nutrition, photosynthesis and transpiration responses of longleaf pine seedlings to light, water and nitrogen 总被引：15，自引：0，他引：15
Early growth and physiology of longleaf pine (Pinus palustris Mill.) seedlings were studied in response to light, water and nitrogen under greenhouse conditions. The experiment was conducted with 1-year-old seedlings grown in 11.3 l pots. The experimental design was a split-plot factorial with two levels (low and high) of each of the factors, replicated in three blocks. The four factorial combinations of water and nitrogen were randomly applied to 15 pots (sub-plots) in each of the light treatment (main plot). Data were collected on survival, root collar diameter (RCD), and height on a monthly basis. Biomass (shoot, root and needle), leaf area index, specific needle area, and needle nutrient (N, P, K, Ca, and Mg) concentrations were determined following final harvest after 16 months. Physiological data (net photosynthesis and transpiration) were collected monthly from March to July during the second growing season.
Height and RCD were significantly influenced by nitrogen and water and by the interaction between them with no apparent effect of light. Seedlings grew 93% taller in the high nitrogen and well watered (HNWW) treatment compared to the low nitrogen and water stressed (LNWS) treatment. Similarly, a significant increase (78%) in RCD was observed for seedlings in the HNWW treatment over the LNWS treatment. Light, along with water and nitrogen, played an important role in seedling biomass growth, especially when water was not limiting. Biomass partitioning (as measured by root:shoot ratio) was affected only by nitrogen and water. Nutrient stress had a greater influence on carbon allocation (69% increase in root:shoot ratio) than water stress (19% increase). Net photosynthesis (Pnet) was significantly higher for seedlings in the high resource than in the low resource treatments with significant light×water and nitrogen×water interactions. Transpiration rate was higher (75%) under the WW treatment compared to the WS treatment. Longleaf pine seedlings grown under the LNWW treatment had the lowest foliar nitrogen (0.71%) whereas seedlings in the HNWS treatment had the highest (1.46%). Increasing the availability of light (through larger canopy openings or controlling midstory density) and soil nitrogen (through fertilization) may not result in greater Pnet and improved seedling growth unless soil water is not limiting. 相似文献