共查询到20条相似文献，搜索用时 31 毫秒
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Estrogen-receptor interaction 总被引：27，自引：0，他引：27
The interaction of estradiol with uterine cells involves the association of the hormone with an extranuclear receptor protein, followed by temperature dependent translocation of the resulting complex to the nucleus. During this process, the steroid binding unit of the protein undergoes an alteration, called "receptor transformation," that can be recognized by an increase in its sedimentation rate from 3.8S to 5.2S, and by its acquisition of the ability to bind to isolated uterine nuclei and to alleviate a tissue specific deficiency in the RNA synthesizing capacity of such nuclei. Receptor transformation can be effected in the absence of nuclei by warming uterine cytosol with estradiol. This preparation of transformed complex resembles that extracted from nuclei both in its sedimentation rate (5.3S) and in its ability to bind to uterine nuclei and augment RNA synthesis, properties that are not shown by the native complex. It is proposed that receptor transformation is an important step in estrogen action and that a principal role of the hormone is to induce conversion of the receptor protein to a biochemically functional form. 相似文献
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Negative regulation by glucocorticoids through interference with a cAMP responsive enhancer 总被引：65，自引：0，他引：65
I E Akerblom E P Slater M Beato J D Baxter P L Mellon 《Science (New York, N.Y.)》1988,241(4863):350-353
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Correlation of glucocorticoid receptor binding sites on MMTV proviral DNA with hormone inducible transcription 总被引：9，自引：0，他引：9
M Pfahl D McGinnis M Hendricks B Groner N E Hynes 《Science (New York, N.Y.)》1983,222(4630):1341-1343
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Glucocorticoid receptors in lymphoma cells in culture: relationship to glucocorticoid killing activity 总被引：17，自引：0，他引：17
Mouse lymphoma cells in culture which are killed by adrenal steroids contain specific cortisol receptors that may be involved in the initial events of hormone action. The similarity of these receptors to those in hepatoma tissue culture cells, where adrenal steroids induce tyrosine aminotransferase, suggests that certain aspects of steroid action are similar in the two systems. In three steroid-resistant lymphoma cell populations specific binding was less than in the parent lines, suggesting that conversion to steroid resistance may be associated with changes in specific steroid binding. 相似文献
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Receptor-associated resistance to growth hormone-releasing factor in dwarf "little" mice 总被引：5，自引：0，他引：5
Anterior pituitaries from the dwarf mouse strain "little" did not release growth hormone or accumulate adenosine 3',5'-monophosphate (cyclic AMP) in response to human and rat growth hormone-releasing factor (GRF). Dibutyryl cyclic AMP, as well as the adenylate cyclase stimulators forskolin and cholera toxin, markedly stimulated growth hormone (GH) release. The basis of the GH deficiency in the little mouse may therefore be a defect in an early stage of GRF-stimulated GH release related either to receptor binding or to the function of the hormone-receptor complex. 相似文献
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Protein synthesis: its control in erythropoiesis 总被引：16，自引：0，他引：16
Erythropoiesis in the fetal mouse provides a model to study several important aspects of the regulation of cell differentiation and differentiated protein synthesis. Changes in the patterns of hemoglobins formed during fetal and postfetal development are shown to be associated with the substitution of the liver erythroid cell line. In the course of differentiation of yolk sac erythroid cells there are at least two classes of proteins distinguishable with respect to dependence on continued RNA formatoin. The bulk of nuclear proteins, "nondifferentiated" proteins, appear to be dependent on relatively short-lived messenger RNA while synthesis of differentiated proteins, the hemoglobins, proceeds on relatively stable molecules of messenger RNA. Hemoglobin formation occurs in those cells which are actively synthesizing DNA and dividing. On the average, two to three cell divisions may occur after the formation and stabilization of the messenger RNA for globin. Yolk sac erythropoiesis, at least from day 10 of gestation, is unresponsive to erythropoietin. By comparison, in fetal liver erythropoiesis, the hormone, erythropoietin, acts selectively on the most immature erythroid cell precursor to induce differentiation, cell replication, and hemoglobin formation. The erythropoietin responsive cell in the liver is apparently differentiated from the progenitor, pluripotential stem cell and committed to erythroblast formation and hemoglobin synthesis on exposure to the hormone. The initial effects of erythropoietin on macromolecular synthesis are to stimulate RNA synthesis, which temporally is followed by cell replication and the increase in hemoglobin formation. During liver erythropoiesis, there appears to be a transition from hemoglobin synthesis dependent on RNA formation to hemoglobin synthesis directed by relatively stable messenger RNA. 相似文献
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Estrogen memory effect in human hepatocytes during repeated cell division without hormone 总被引：3，自引：0，他引：3
Transient stimulation of target tissues by sex steroids can cause long-lasting changes that may facilitate or alter responses to subsequent hormonal treatment. How these altered characteristics are propagated during cell division in the absence of the stimulating hormone is unknown. The human hepatocarcinoma cell line HepG2 was used as a model to examine the effects of estrogen on the synthesis of serum apolipoproteins in vitro. Treatment with low concentrations of estrogen for 24 to 48 hours resulted in long-lasting alterations in the kinetics with which the cells responded to subsequent stimulation with estrogen. Manifestation of this memory effect was correlated quantitatively with the induction and propagation of a moderate-affinity, nuclear, estrogen-binding protein with the characteristics of a type II estrogen receptor. The data indicate that transient exposure of these cells to estrogen can induce changes in their response characteristics and composition of nuclear proteins that are inherited by daughter cells grown in the absence of hormone for more than ten generations. 相似文献
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Participation of the TATA factor in transcription of the yeast U6 gene by RNA polymerase C 总被引：35，自引：0，他引：35
F Margottin G Dujardin M Gérard J M Egly J Huet A Sentenac 《Science (New York, N.Y.)》1991,251(4992):424-426
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Circular RNA(circRNA)是一类在转录过程中形成的首尾相接的环状非编码RNA,其在多种生物学过程以及疾病发生过程中发挥重要作用。基于前期鸭胚原代脂肪细胞分化过程中circRNA测序结果,本研究选择由FBLN2编码的circ-FBLN2进行验证分析。通过PCR扩增和焦磷酸测序技术获得接合序列,采用Rnase R消化实验进一步验证了circ-FBLN2的存在,采用核质分离技术得出circ-FBLN2主要存在于细胞质中,且其在脂肪细胞诱导分化后显著下调,在樱桃谷鸭各组织中的表达具有一定的差异,主要在脂肪和腿肌中表达。生物信息学分析结果显示,circ-FBLN2与预测到的靶标基因主要参与MAPK,Toll样受体,以及细胞因子与受体互作通路等信号通路,揭示其可能通过对靶基因的调控作用间接调控鸭脂肪细胞分化过程。 相似文献
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Actinomycin D: inhibition of protein synthesis unrelated to effect on template RNA synthesis 总被引：18，自引：0，他引：18
Incubation of sarcoma-37 ascites cells in vitro with actinomycin D resulted in inhibition of synthesis of nuclear and cytoplasmic proteins. The overall inhibition could be prevented or relieved by glucose; it is thus unrelated to breakdown of template RNA. 相似文献
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【目的】卵巢颗粒细胞的增殖和分化是原始卵泡生长启动的关键因素,卵巢颗粒细胞过度凋亡是卵泡闭锁的主要原因,因此卵巢颗粒细胞的功能对卵泡生长发育、排卵、激素分泌等至关重要。研究通过探究连环蛋白β1（catenin beta 1, CTNNB1）对猪卵泡发育过程中颗粒细胞的增殖、凋亡及类固醇激素分泌等功能的影响,为猪卵泡发育的分子调控机制研究提供参考。 【方法】利用RNA抽提、实时定量PCR（Quantitative real time PCR, qRT-PCR）等方法,构建CTNNB1在母猪卵巢、肌肉、大脑等9个组织的表达谱;检测母猪性成熟过程中卵巢组织和小（≤3 mm）、中（3—6 mm）、大（≥6 mm）卵泡颗粒细胞中CTNNB1的表达情况。构建CTNNB1的真核表达载体及合成siRNA,转染至猪卵巢颗粒细胞,采用EdU、Annexin V- FITC/PI双染、ELISA等方法,检测CTNNB1对颗粒细胞增殖、凋亡、类固醇激素分泌的影响,以及对类固醇激素合成通路重要基因转录表达的影响。【结果】与肌肉、大脑等组织相比,CTNNB1在卵巢中mRNA表达水平最高。性成熟母猪卵巢中CTNNB1转录水平显著高于性成熟前和性成熟后的母猪;卵巢卵泡中CTNNB1转录和蛋白水平随卵泡发育明显上调;且卵巢颗粒细胞中CTNNB1转录和蛋白水平也随卵泡的发育逐渐上调。更重要的是,CTNNB1显著促进颗粒细胞增殖,抑制颗粒细胞凋亡;CTNNB1能够上调CYP1A1和HSD17B7的转录水平,下调CYP11A1、ESR1、ESR2、FSHR、LHR和NR5A1等类固醇激素合成相关基因的转录水平,进而促进颗粒细胞雌激素的分泌,抑制颗粒细胞雄激素和孕激素的分泌。【结论】本研究证实CTNNB1可能通过促进颗粒细胞增殖及雌激素的合成和分泌,抑制颗粒细胞凋亡及雄激素、孕激素的合成和分泌,进而促进卵泡的生长发育。 相似文献