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1.
爆裂玉米3个膨爆特性的非条件和条件QTL分析 总被引：2，自引：0，他引：2
膨化倍数(PF)、膨化体积(PV)和爆花率(PR)是爆裂玉米的主要品质指标。本研究以普通玉米自交系丹232和爆裂玉米自交系N04杂交构建的259个F2:3家系为定位群体,采用完全随机区组设计在郑州夏播条件下测定了3个膨爆特性指标。利用覆盖玉米10条染色体的183对多态性分子标记构建连锁图,采用复合区间作图(CIM)方法对各性状进行非条件QTL定位分析,采用条件遗传统计软件对PF进行条件QTL分析。3个膨爆特性指标非条件QTL定位共检测出31个QTL,单个QTL的贡献率为3.09%￣12.46%,累计贡献率为51.90%、77.37%和61.45%。在8个标记区间(占42.11%)同时检测到控制2￣3个性状的QTL。加性和部分显性在膨爆特性的遗传中起主要作用,同时存在显性和超显性基因效应。条件QTL定位结果表明,PV和PR以不同方式显著影响PF的QTL表达,PV比PR更重要。 相似文献
2.
利用相同来源F2:3和BC2S1群体定位玉米生育期QTL 总被引：1，自引：0，他引：1
以普通玉米自交系丹232和爆裂玉米自交系N04为亲本构建259个F2:3和220个BC2S1家系群体,利用SSR标记构建分子标记遗传图谱,利用复合区间作图方法对4个生育期性状进行QTL定位和效应分析。利用F2:3群体共检测到4个抽雄期QTL、6个吐丝期QTL和3个散粉期QTL。单个QTL可解释的表型变异为6.7%~18.4%,可解释的表型总变异为28.9%~50.3%,11个QTL的增效基因来自生育期较长的亲本丹232,其余2个QTL的增效基因来自生育期较短的亲本N04;BC2S1群体检测到8个与4个生育期性状相关的QTL,单个QTL可解释的表型变异为4.5%~11.6%,可解释的表型总变异为13.2%~18.5%,增效基因来自两个亲本的QTL为3个和5个。两类群体检测出QTL的数目、位置、效应和贡献率均存在较大差异,主要原因在于BC2S1群体抽样选择所引起的群体结构差异,F2:3群体显示出较高的QTL检测能力,但回交育种过程中应慎重依据F2:3群体QTL定位结果进行标记辅助选择(MAS)。 相似文献
3.
QTL for popping characteristics in popcorn 总被引：1，自引：0，他引：1
Popping volume (PV), flake size (FS) and popping rate (PR) are three important popping characteristics in popcorn ( Zea mays L.). We developed 259 F2:3 lines from a cross between a dent corn inbred 'Dan232' and a popcorn inbred 'N04' that were grown during spring and summer to evaluate the three popping characteristics. Fifteen quantitative trait loci (QTL) were detected on chromosomes 1, 2, 5, 6, 7 and 8 using composite interval mapping. The numbers of QTL were 6, 4 and 5 for PV, FS and PR, respectively, with phenotypic variance explained ranging from 4.9% to 15.6% for a single QTL, accounting for a total of 54.0%, 34.5% and 39.1% of the total phenotypic variance respectively. Partially dominance and overdominance effects played an important role in popping characteristics. Only 13 pairs of digenic interactions were detected. The QTL on chromosome bin 1.05–1.06 for PV and PR, and 5.07 for FS were in accordance with previous reports and could be good candidate QTL for marker-assisted selection. There was a significant correlation amongst QTL on chromosomes 1, 6 and 8 for PV and PR. 相似文献
4.
利用高代回交方法定位爆裂玉米膨化倍数QTL 总被引：8，自引：0，他引：8
高代回交QTL分析方法可以使QTL检测和育种相结合,有效利用非适应性种质。在河南郑州春、夏播两种环境条件下,种植以普通玉米自交系丹232和爆裂玉米自交系N04为亲本构建的220个BC2S1家系群体,利用覆盖玉米10条染色体的188对多态性SSR分子标记,采用单标记分析法对控制膨化倍数QTL进行分析。目的在于印证利用F2:3家系的QTL分析结果,检测具有世代稳定性的QTL,同时将QTL分析与爆裂玉米育种相结合,直接从中选育爆裂玉米自交系。研究结果表明,膨化倍数在家系间、环境间均存在极显著差异,家系与环境互作不显著。BC2S1家系呈连续正态分布,且存在超高亲分离,超高亲家系分别占12.27%和19.09%。共检测出16个QTL,其中2个在两种环境条件下均被检测到,单个QTL的贡献率为3%~9%,累计贡献率为42%和33%,增效基因均来自爆裂N04。BC2S1家系的穗粒和植株性状均得到明显改良,各性状优于爆裂亲本N04的家系占10%~100%。两种环境条件下分别有186个和204个家系的穗粒重比爆裂亲本N04高10%以上,其中23个和46个家系的膨化倍数达到或优于N04,可以直接从中选育出优良爆裂玉米自交系。 相似文献
5.
Dent corn genetic background influences QTL detection for grain yield and yield components in high-oil maize 总被引：3，自引：0，他引：3
Despite the well-recognized importance of grain yield in high-oil maize (Zea mays L.) breeding and production, few studies have reported the application of QTL mapping of such traits. An inbred line of high-oil
maize designated ‘GY220’ was crossed with two dent maize inbred lines to generate two connected F2:3 populations with 284 and 265 F2:3 families. Our main objective was to evaluate the influence of genetic background on QTL detection of grain yield traits through
comparisons between the F2:3 populations. The field experiments were conducted during the spring in Luoyang and summer in Xuchang, Henan, China. Two genetic
linkage maps were constructed with a genetic distance of 2111.7 and 2298.5 cM using 185 and 173 polymorphic SSR markers, respectively.
In total, 18 and 15 QTL were detected for six grain yield traits in the two populations. Only one common QTL marker was shared
between the two populations. A QTL cluster associated with five traits was identified at bin 1.05–1.06, including the shared
QTL for 100GW, which demonstrated the largest effect (16.7%). Among the detected QTL, 12 digenic interactions were identified.
Our results reflect the substantial influence of dent maize genetic background on QTL detection of grain yield traits. 相似文献
6.
利用普通玉米自交系丹232与爆裂玉米自交系N04为亲本构建的重组近交系(RILs) 群体为材料,对籽粒不同发育时期的粗蛋白、粗淀粉、粗脂肪和赖氨酸4个品质性状分析结果表明：RILs群体各时期4个品质性状均存在明显的超亲分离;亲本、F1及RILs群体的粗蛋白、粗脂肪及赖氨酸含量从10 DAP至30 DAP持续下降,至灌浆后期又表现不同程度的升高,而粗淀粉含量在整个发育时期持续增加。除粗蛋白的10 DAP与40 DAP间、粗脂肪的10 DAP与30 DAP间不相关外,其余各性状在不同发育时期均表现为显著或极显著正相关。粗淀粉与粗蛋白、粗脂肪及赖氨酸在4个时期表现为极显著负相关,其余性状间均表现为极显著正相关。 相似文献
7.
In order to detect quantitative trait loci (QTLs) for drought tolerance in wheat during seed germination conditional and unconditional QTL analyses of eight seedling traits were conducted under two water regimes using three related F9 recombinant inbred line populations with a common female parent. A total of 87 QTLs for the eight seedlings traits and 34 specific QTLs related to drought tolerance were detected. Seventy-one of these QTLs were major QTLs with contributions to phenotypic variance of >10 %. Of the 34 QTLs related to drought tolerance only eight were also detected by unconditional analysis of seedling traits under osmotic stress conditions indicating that most of the QTLs related to drought tolerance could not be detected by unconditional QTL analysis. Therefore, conditional QTL analysis of stress-tolerance traits such as drought tolerance was feasible and effective. Of 11 important QTL clusters located on chromosomes 1BL, 1D, 2A, 2B, 2D, 4A, 6B, and 7B, nine were detected in multiple populations and eight were detected by both unconditional and conditional analyses. 相似文献
8.
QTL analysis for grain weight in common wheat 总被引：6，自引：0，他引：6
Neeraj Kumar Pawan L. Kulwal Anupama Gaur Akhilesh K. Tyagi Jitendra P. Khurana Paramjit Khurana Harindra S. Balyan Pushpendra K. Gupta 《Euphytica》2006,151(2):135-144
Quantitative trait loci (QTL) analysis for grain weight (GW = 1000 grain weight) in common wheat was conducted using a set of 100 recombinant inbred lines (RILs) derived from a cross ‘Rye Selection 111 (high GW) × Chinese Spring (low GW)’. The RILs and their two parental genotypes were evaluated for GW in six different environments (three locations × two years). Genotyping of RILs was carried out using 449 (30 SSRs, 299 AFLP and 120 SAMPL) polymorphic markers. Using the genotyping data of RILs, framework linkage maps were prepared for three chromosomes (1A, 2B, 7A), which were earlier identified by us to carry important/major genes for GW following monosomic analysis. QTL analysis for GW was conducted following genome-wide single marker regression analysis (SMA) and composite interval mapping (CIM) using molecular maps for the three chromosomes. Following SMA, 12 markers showed associations with GW, individual markers explaining 6.57% to 10.76% PV (phenotypic variation) for GW in individual environments. The high grain weight parent, Rye Selection111, which is an agronomically superior genotype, contributed favourable alleles for GW at six of the 12 marker loci identified through SMA. The CIM identified two stable and definitive QTLs, one each on chromosome arms 2BS and 7AS, which were also identified through SMA, and a third suggestive QTL on 1AS. These QTLs explained 9.06% to 19.85% PV for GW in different environments. The QTL for GW on 7AS is co-located with a QTL for heading date suggesting the occurrence of a QTL having a positive pleiotropic effect on the two traits. Some of the markers identified during the present study may prove useful for marker-assisted selection, while breeding for high GW in common wheat. 相似文献
9.
玉米光周期敏感相关性状发育动态QTL定位 总被引：2，自引：1，他引：1
玉米是短日照作物,大多数热带种质对光周期非常敏感。光周期敏感性限制了温、热地区间的种质交流。研究玉米光周期敏感性的分子机理,有利于玉米种质的扩增、改良、创新,提高玉米品种对不同光周期变化的适应性。本研究以对光周期钝感的温带自交系黄早四和对光周期敏感的热带自交系CML288为亲本配置的组合衍生的一套207个重组自交系为材料,在长日照环境条件下对不同发育时期的叶片数、株(苗)高变化进行QTL分析。结果表明,双亲间的最终可见叶片数和株高差异很大;发育初期CML288的叶片数和苗高都低于黄早四,而发育后期CML288的叶片数和株高都明显高于黄早四;测定各时期F7重组自交系间也存在显著差异。利用包含237个SSR标记、图谱总长度1 753.6 cM、平均图距7.40 cM的遗传连锁图谱,采用复合区间作图法,分别检测到控制叶片数和株(苗)高发育的QTL 11个和20个。但是,没有一个条件QTL 能在测定的几个时期都有效应。在长日照条件下,控制叶片数与株(苗)高的非条件与条件QTL主要集中在第1、9和10染色体上,特别是在第10染色体的标记umc1873附近均检测到了影响这两个性状的QTL,且在不同的发育时期单个条件和非条件QTL所解释的表型变异分别为4.34%~25.74%和10.02%~22.57%,表明这一区域可能包含光周期敏感性关键基因。 相似文献
10.
Identification of QTL underlying the filling rate of protein at different developmental stages of soybean seed 总被引：1，自引：0，他引：1
Zhenfeng Jiang Yingpeng Han Weili Teng Zhongchen Zhang Desheng Sun Yuhua Li Wenbin Li 《Euphytica》2010,175(2):227-236
Seed protein content at the harvest stage is the sum of protein accumulation during seed filling. The aim of our investigation
was to identify loci underlying the filling rate of seed protein at different developmental stages. To this end, we used 143
recombinant inbred lines (RILs) derived from the cross of soybean cultivars ‘Charleston’ and ‘Dongnong 594’ and composite
interval mapping with a mixed genetic model. The genotype × environment interactions of the quantitative trait loci (QTL)
were also evaluated. Thirty-nine unconditional QTL underlying the filling rate of seed protein at five developmental stages
were mapped onto 14 linkage groups. The proportion of phenotypic variation explained by these QTL ranged from 4.88 to 26.05%.
Thirty-eight conditional QTL underlying the filling rate of seed protein were mapped onto 16 linkage groups. The proportion
of phenotypic variation explained by these QTL ranged from 1.87 to 31.34%. The numbers and types of QTL and their genetic
effects on the filling rate of seed protein were different at each developmental stage. A G × E interaction effect was observed
for some QTL. 相似文献
11.
Analysis of contributors to grain yield in wheat at the individual quantitative trait locus level 
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下载免费PDF全文 Da‐Bin Hou Guo‐Yue Chen Zhi‐En Pu Wei Li Xiu‐Jin Lan Qian‐Tao Jiang Ya‐Xi Liu Mei Deng Yu‐Ming Wei 《Plant Breeding》2018,137(1):35-49
In wheat, strong genetic correlations have been found between grain yield (GY) and tiller number per plant (TN), fertile spikelet number per spike (FSN), kernel number per spike (KN) and thousand‐kernel weight (TKW). To investigate their genetic relationships at the individual quantitative trait locus (QTL) level, we performed both normal and multivariate conditional QTL analysis based on two recombinant inbred lines (RILs) populations. A total of 79 and 48 normal QTLs were identified in the International Triticeae Mapping Initiative (ITMI)/SHW‐L1 × Chuanmai 32 (SC) populations, respectively, as well as 55 and 35 conditional QTLs. Thirty‐two QTL clusters in the ITMI population and 18 QTL clusters in the SC population explained 0.9%–46.2% of phenotypic variance for two to eight traits. A comparison between the normal and conditional QTL mapping analyses indicated that FSN made the smallest contribution to GY among the four GY components that were considered at the QTL level. The effects of TN, KN and TKW on GY were stronger at the QTL level. 相似文献
12.
Yanan Liu Hongwei Zhang Xuhui Li Feng Wang Demar Lyle Lianjun Sun Guoying Wang Jianhua Wang Li Li Riliang Gu 《Plant Breeding》2019,138(1):29-37
Quantitative trait locus (QTL) mapping for seed longevity is essential for breeding modern cultivars with resistance to deterioration during postharvest storage. The inbred lines X178 and I178 showed large differences in seed vigour after artificial aging treatment, while they had similar performances in terms of most agronomic traits. An F2:3 population and a recombinant inbred line (RIL) population were generated to map QTL after 5 days under artificial aging conditions. Positive correlations were observed among all investigated traits including the aging germination rate, relative aging germination rate, aging simple vigour index, aging primary root length, aging shoot length and aging total length. Thirteen QTL were identified to locate on five chromosome regions: Chr.1:297 Mb (chromosome 1 region 297 Mb), Chr.3:205 Mb, Chr.4:240 Mb, Chr.5:205 Mb and Chr.7:155 Mb, with 2 to 4 QTL co‐located on a region. In each region, 3–8 previously identified aging‐related QTL were located, confirming the importance of these regions for controlling seed longevity in different maize populations. Taken together, the results of this work provide a foundation for further QTL fine mapping and the molecular‐assisted breeding of aging tolerant maize. 相似文献
13.
Yingpeng Han Dongmei Li Guiyun Zhao Xue Zhao Zhenfeng Jiang Haibo Hu Lin Wu Yan Wang Yuan Gao Yongguang Li Guoliang Zeng Fanli Meng Weili Teng Wenbin Li 《Plant Breeding》2016,135(3):335-341
Most of quantitative trait loci (QTL) underlying soybean seed isoflavone contents were derived from the harvest stage of plant development, which uncover the genetic effects that were expressed in earlier seed developmental stages. The aim of this study was to detect conditional QTL associated with isoflavone accumulation during the entire seed development. A total of 112 recombinant inbred lines developed from the cross between ‘Zhongdou 27’ (higher seed isoflavone content) and ‘Jiunong 20’ (lower seed isoflavone content) were used for the conditional QTL analysis of daidzein (DZ), genistein (GT), glycitein (GC) and total isoflavone (TI) accumulations through composite interval mapping with mixed genetic model. The results indicated that the number and type of QTL and their additive effects for individual and total isoflavone accumulations were different among R3 to R8 developmental stages. Three unconditional QTL and six conditional QTL for DZ, four unconditional QTL and five conditional QTL for GT, six unconditional QTL and five conditional QTL for GC, six unconditional QTL and seven conditional QTL for TI were identified at different developmental stages, respectively. Unconditional and conditional QTL that affect individual and total isoflavone accumulations exhibited multiple expression patterns, implying that some QTL are active for long period and others are transient. Two genomic regions, Satt144‐Satt569 in linkage group F (LG F; chromosome 13, chr 13) for DZ, GC, GT and TI accumulations and Satt540‐Sat_240 in LG M (chr 07) for TI and GC accumulations, were found to significantly affect individual and total isoflavone accumulations in multiple developmental stages, suggesting that the accumulation of soybean seed isoflavones is governed by time‐dependent gene expression. 相似文献
14.
Construction of chromosomal segment substitution lines and genetic dissection of introgressed segments associated with yield determination in the parents of a super‐hybrid rice 下载免费PDF全文
下载免费PDF全文 Xi Liu Zhigang Zhao Linglong Liu Yinghui Xiao Yunlu Tian Shi‐jia Liu Liangming Chen Yihua Wang Yuqiang Liu Saihua Chen Wenwei Zhang Chunming Wang Ling Jiang Jianmin Wan 《Plant Breeding》2016,135(1):63-72
Heterosis is a phenomenon whereby hybrids of inbred lines produce favourable phenotypes that exceed those of their parents. Traits of interest are higher yield and stronger stress tolerance. The two‐line super‐hybrid rice ‘Liangyoupei9’ (LYP9) shows superiority to both its elite inbred line ‘93‐11’ and ‘Pei'ai64s’ (‘PA64s’) parents and conventional hybrids. However, the genetic basis of its hybrid vigour, especially yield determination, remains elusive. In the present study, a set of 156 chromosome segment substitution lines (CSSLs) carrying overlapping segments from ‘PA64s’ in a genetic background of ‘93‐11’ were constructed and planted in six environments. Three major agronomic traits, viz. panicle length (PL), heading date (HD) and plant height (PH), and five yield‐related traits, viz. grain weight per panicle (GWP), number of grains per panicle (GPP), 1000‐grain weight (TGW), seed set (SS) and number of panicles of per plant (PPP), were evaluated over 3 years. Quantitative trait loci (QTL) analysis was conducted using a likelihood ratio test based on stepwise regression. Forty‐six putative QTL distributed on 11 chromosomes were detected in more than one year. Remarkably, GWP of four CSSLs carrying positive yield QTL outperformed the recurrent parent ‘93‐11’ by more than 15%, in at least two environments. These results indicate that CSSLs are effective in identifying yield‐associated traits, and lines harbouring such QTL will be rich in resources for future molecular breeding programmes. 相似文献
15.
16.
Dissection of component QTL expression in yield formation in rice 总被引：10，自引：0，他引：10
L. B. Guo Y. Z. Xing H. W. Mei C. G. Xu C. H. Shi P. Wu L. J. Luo 《Plant Breeding》2005,124(2):127-132
Yield and its components were investigated by using a population of 241 recombinant inbred lines (F9 RILs) derived from an elite hybrid rice cross of ‘Zhenshan 97’בMinghui 63′. Quantitative trait loci (QTLs) for causal analysing of yield traits were detected at different yield component (YC) influences by conditional and unconditional QTL mapping methods. The number of QTLs significantly affecting yield was different at component‐special influence. Some QTLs controlling yield identified in one component influence were undetectable at the others. More QTLs for yield could be detected at different YC influences. It is possible to reveal that causal gene expression for yield could be different at different YC influences. Mapping QTLs for component effects of yield could help us in understanding the nature of cause‐effect traits for the formation of grain yield. 相似文献
17.
基于SSR-PCR标记的不同种群玉米种质遗传多样性研究 总被引：3，自引：0，他引：3
利用SSR-PCR分子标记对硬粒型、马齿型、半马齿型、爆裂型、甜质型、糯质型6个种群共72份玉米种质的遗传多样性进行了分析研究。57对SSR引物在供试材料中共计检测出222个等位基因位点,每对引物检测出2-8个等位基因,平均3.89个。试验材料间Nei’S遗传距离变幅为0.0793-0.6637;爆裂型、甜质型、糯质型3个特用玉米种群内材料间平均遗传距离明显小于种群间平均遗传距离,3个普通玉米种群（硬粒型、马齿型、半马齿型）则表现为种群内、种群间平均遗传距离无明显差异;特用玉米种质间遗传距离变异幅度总体上小于普通玉米种质间以及特用玉米与普通玉米间遗传距离的变异幅度。根据UPGMA法,将18个特用玉米自交系分为三个组,与其种群划分基本相符。试验用大部分普通玉米种质均可划入以标准测验种为代表的5个杂种优势类群中,而又以PB群、旅大红骨群和BSSS群为主,分别占31.5%、16.7%和14.8%。 相似文献
18.
Increasing seed oil content is an important breeding goal for Brassica napus L. (B. napus). The identification of quantitative trait loci (QTL) for seed oil content and related traits is important for efficient
selection of B. napus cultivars with high seed oil content. To get better knowledge on these traits, a molecular marker linkage map for B. napus was constructed with a recombinant inbred lines (RIL) population. The length of the map was 1,589 cM with 451 markers distributed
over 25 linkage groups. QTL for seed oil content, seed hull content and seed coat color in three environments were detected
by composite interval mapping (CIM) tests. Eleven QTL accounted for 5.19–13.57% of the variation for seed oil content. Twelve
QTL associated with seed hull content were identified with contribution ranging from 5.80 to 22.71% and four QTL for seed
coat color accounted for 5.23–15.99% of the variation. It is very interesting to found that co-localization between QTL for
the three traits were found on N8. These results indicated the possibility to combine favorable alleles at different QTL to
increase seed oil content, as well as to combine information about the relationship between seed oil content and other traits. 相似文献
19.
Ma Yu Shuang-Lin Mao Guo-Yue Chen Zhi-En Pu Yu-Ming Wei You-Liang Zheng 《Euphytica》2014,200(1):95-108
Spike length (SL) is one of the most important components of spike morphology, and the uppermost internode represents an ideal organ to study the transportation system. We performed conditional and unconditional quantitative trait locus (QTL) mapping in two unrelated recombinant inbred line populations to precisely detect QTLs for uppermost internode length (UIL) and SL, and to dissect the genetic relationship between these two factors with plant height (PH). Both of the populations were derived from crosses with synthetic wheat. Ten repetitive QTLs for UIL and six environment-independent QTLs for SL were identified in this study, and twelve of these were completely independent of PH. Conditional QTL mapping analysis indicated that SL was more independent to PH than UIL was. The results indicated that the conditional QTL mapping method could evaluate PH component effects on PH, and thus accelerate the selection of suitable loci that improve commercial wheat morphology yet avoid changes to PH. 相似文献