共查询到18条相似文献，搜索用时 156 毫秒
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《食用菌学报》2017,(4)
以雄性小白鼠为研究对象,用四氯化碳(carbon tetrachloride,CCl4)诱导的小鼠肝损伤模型,测定红托竹荪(Dictyophora rubrovalvata)对小鼠血清中谷丙转氨酶(alanine aminotransferase,ALT)、谷草转氨酶(aspartate aminotransferase,AST)和碱性磷酸酶(alkaline phosphatase,ALP)水平的影响,对小鼠肝组织进行病理学检查。结果表明,与模型组相比,红托竹荪组小鼠血清中ALT、AST和ALP含量降低,组织病变程度减轻,红托竹荪菌柄水提液对CCl_4诱导的小鼠肝损伤具有保护作用。 相似文献
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亮菌多糖ATPS-2对小鼠四氯化碳和酒精肝损伤的保护作用 总被引：2，自引：0，他引：2
分别采用四氯化碳（CCl4）和北京红星二锅头诱导小鼠肝损伤，比色法测定血清中丙氨酸氨基转移酶（ALT）、天门冬氨酸氨基转移酶（AST）的含量；肝脏中超氧化物歧化酶（SOD）活力和丙二醛（MDA）浓度，并作肝组织切片病理观察。亮菌多糖ATPS-2（25mg／kg、50mg／kg、100mg／kg）给药明显降低小鼠血清中升高的ALT和AST水平，抑制肝脏中上升的MDA水平和提高过低的SOD活性。病理检查结果显示亮菌多糖ATPS-2有明显的保肝作用。亮菌多糖ATPS-2对小鼠四氯化碳肝损伤和酒精肝损伤均具有显著的保护作用。 相似文献
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《中国食用菌》2016,(6)
探讨灵芝(Ganoderma lucidum)孢子油提取物复合制剂对小鼠急性酒精肝损伤的保护作用。分别设定0.167 g·kg~(-1)、0.333 g·kg~(-1)、1.00 g·kg~(-1)三个剂量组,以及乙醇模型对照组和空白对照组。各剂量组经口灌胃给予受试物,溶剂对照组和乙醇模型组给予食用植物油。30 d后,用乙醇建立急性酒精性肝损伤小鼠模型。16 h后处死动物,测定肝组织中甘油三酯(TG)、谷胱甘肽(GSH)、丙二醛(MDA),同时取肝脏进行组织病理学检查。结果表明样品可以降低3个剂量组小鼠急性酒精性肝损伤后第16小时肝组织中TG含量和中剂量组MDA含量,肝脏组织病理学检查发现,乙醇模型对照组动物肝损伤程度高于溶剂对照组(P0.05)。结论证明灵芝孢子油提取物复合制剂对急性酒精肝损伤具有辅助保护功能。 相似文献
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采用正交试验优化超声波辅助提取尖顶羊肚菌(Morchella conica)多糖工艺,并分析多糖对肝损伤小鼠体内抗氧化活性.结果表明,在超声功率600 W、提取温度60℃、提取时间80 min、醇沉浓度70％、料液比1∶40(g∶mL)的条件下,多糖得率为(2.25±0.04)％.影响多糖得率的因素由大到小依次为超声功率、提取温度、料液比、乙醇浓度和提取时间.与CCl4诱导的小鼠急性肝损伤模型组相比,灌胃尖顶羊肚菌多糖处理组的肝组织中丙二醛含量显著降低、总抗氧化能力显著提高;血清中丙二醛含量显著降低、超氧化物歧化酶活性和总抗氧化能力显著增加. 相似文献
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《中国食用菌》2015,(4)
评价猴头菌H10的水提物对酒精造成的胃粘膜损伤和肝损伤的保护作用。采用95%乙醇造成大鼠急性胃粘膜损伤,测定胃粘膜损伤指数;采用50%乙醇造成小鼠急性肝损伤,测定小鼠血清甘油三脂、极低密度脂蛋白、门冬氨酸转移酶、丙氨酸氨基转移酶含量,测定小鼠肝组织中丙二醛、超氧化物歧化酶、谷胱甘肽过氧化物酶含量。猴头菌中剂量组和大剂量组能明显降低乙醇造成的大鼠胃粘膜损伤指数;猴头低剂量组能明显降低小鼠肝损伤动物血清VLDL含量,中剂量能够降低ALT、VLDL、MDA含量,高剂量组能够降低ALT、AST、VLDL、TG、MDA含量及提高SOD、GSH-Px活性。猴头菌对酒精造成的胃粘膜损伤和肝损伤具有保护作用。 相似文献
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AIM: To explore the protective effect of riboflavin preconditioning on hepatic ischemia/reperfusion injury in rats. METHODS: Twenty-four Sprague-Dawley rats wererandomly divided into 3 groups (n=8): sham group, ischemia/reperfusion (I/R) group and riboflavin preconditioning (R+I/R) group. The rats in sham group and I/R group received a standard chow,while the rats in R+I/R group received a chow supplemented with riboflavin. After 4 weeks, portal vein and hepatic artery supplying the middle and left hepatic lobes were clamped with a traumatic vascular clip for induction of partial hepatic ischemia in the rats in I/R group and R+I/R group. After 1 h of ischemia, 1 h of reperfusion was conducted by removal of the clip. The activity of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in serum,the activity of superoxide dismutase (SOD) and the level of malondialdehyde (MDA) in serum and liver were measured. Western blotting was employed to examine the protein expression of heme oxygenase-1(HO-1) in the liver. RESULTS: The results showed that ischemia/reperfusion injury markedly increased the activity of AST and ALT in serum, decreased the activity of SOD, and elevated the level of MDA and the activity of HO-1 in the liver as compared with sham group (P<0.01). The riboflavin pretreatment significantly decreased the activity of AST and ALT in serum, increased the activity of SOD and decreased the levels of MDA in serum and liver as compared with I/R group (P<0.01). In addition, the protein expression of HO-1 and the activity of HO-1 were elevated in R+I/R group (P<0.01). Cytoplasmic vacuolation and swelling of the hepatocytes were observed in I/R group. Treatment with riboflavin markedly alleviated the changes of liver structure. CONCLUSION: Riboflavin preconditioning has protective effect on hepatic ischemia/reperfusion injury. The mechanism may be correlated with enhancing the anti-oxidation and alleviating the reaction of lipid peroxidation. 相似文献
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LI Mei-ai WANG Hua-dong LU Da-xiang WANG Yan-ping QI Ren-bin YAN Yu-xia FU Yong-mei LI Chu-jie 《园艺学报》2006,22(5):987-991
AIM: To investigate the protective effects of berberine against liver injury induced by lipopolysaccharide in mice and the mechanisms underlying its protective effect. METHODS: The male mice were divided randomly into control, berberine group, LPS group and berberine treatment group. Mice were administered intragastrically with distilled water (0.01 mL/g) or 5 g/L neutral sulfate berberine (0.01 mL/g) once a day for 5 days and injected intraperitoneally with normal saline or LPS （0.02 mL/g，28 mg/kg）at 1 h after gavage on day 5. Blood was collected for determining alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities, the content of tumor necrosis factors-α (TNF-α) at 10 h and 2 h after LPS or normal saline injection, respectively. Furthermore, the liver tissue was processed, and histological changes and ultrastructure in liver were observed with light and electron microscopy, malondialdehyde (MDA) content and superoxide dismutase (SOD) activity in liver were also detected. RESULTS: Both ALT and AST activities in serum in LPS group were higher than those in control and berberine treatment group. LPS increased the serum TNF-ɑ content at 2 h after injection, which was reversed by berberine pretreatment. The histological examination showed that LPS caused severe hepatic cell edema, degeneration, apoptosis and even necrosis, and ultrastructure observation demonstrated that LPS induced mitochondrial swelling, condensation and margination of chromatin, irregular nuclear envelope in hepatocytes. The above pathological changes produced by LPS were attenuated by berberine pretreatment. Moreover, MDA contents in liver tissue were higher in LPS group than control and berberine treatment group, but there were no significant difference in SOD activity between berberine treatment and LPS group. CONCLUSION: Berberine has a protective effect on LPS-induced liver injury in mice, the mechanisms may be related to its decreasing the production of TNF-α, inhibiting lipid peroxidation and protecting mitochondria. 相似文献
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AIM: To study the protective effect of aqueous extract of 2-branched and 3-branched velvet antler on cisplatin (CDDP)-induced nephrotoxicity in mice. METHODS:The mouse model of renal injury was induced by intragastric administration of CDDP at the dose of 15 mg/kg. After treatment, kidney index (KI), serum creatinine (SCr), blood urea nitrogen (BUN), the activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), and the content of malondialdehyde (MDA) in the kidney were determined. The renal pathological changes were observed with HE staining. RESULTS: Aqueous extract of velvet antler at the tested dose markedly decreased BUN, SCr and the content of MDA, and elevated the activity of SOD and GSH-Px in the mice pretreated with CDDP (P<0.05). The pathological changes of the renal tissues were improved obviously, and the injury of the epithelial cells of renal tubules was mitigated. The effect of the aqueous extract of 2-branched velvet antler on renal function and cisplatin-induced nephrotoxicity was better than that of 3-branched one at the same concentration. CONCLUSION: The aqueous extract of 2-branched and 3-branched velvet antler has a certain protective effect on cisplatin-induced nephrotoxicity, which may be associated with increasing the anti-oxidative capability of mouse renal tissue. 相似文献
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AIM:To investigate the protective effect of crude Paecilomyces tenuipes polysaccharides (cPtPs) and Paecilomyces tenuipes polysaccharides (PtPs) in a rat model of acute hepatic necrosis induced by carbon tetrachloride (CCl4). METHODS:Wistar rats were divided into four groups (control group, CCl4 group, cPtPs+CCl4 group and PtPs +CCl4 group), the four groups were given intragastrically with normal saline, cPtPs and PtPs for 15 d, respectively. In the last two days, these groups except control group were injected peritoneally with CCl4. Serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin (TBIL), direct bilirubin (DBIL) and indirect bilirubin (IBIL) were detected by automatic biochemistry analyzer. Pathological changes of hepatic tissue were assessed by hematoxylin-eosine (HE) staining. The levels of superoxide dismutase (SOD) and malondialdehyde (MDA) in liver homogenate were analyzed using xanthinoxidase and thio-barbituric acid, respectively. The concentration of Ca2+ in hepatocyte mitochondria was determined by colorimetric method. The expression of α-smooth muscle actin (α-SMA) was examined in hepatic tissue by immunohistochemical method. RESULTS:Compared with control group, serum levels of ALT, AST, TBIL, DBIL and IBIL in CCl4 group increased significantly, denaturation and necrosis implicated to the whole hepatic lobules. Compared with CCl4 group, serum levels of ALT, AST, TBIL, DBIL and IBIL in PtPs +CCl4 group decreased significantly, denaturation and necrosis located in the third region of hepatic lobules, the level of SOD increased and MDA decreased (P<0.05) in endochylema. Concentration of Ca2+ in mitochondria decreased in PtPs +CCl4 group and cPtPs +CCl4 group (P<0.05, P<0.01, respectively). Expression of α-SMA was found little in PtPs+CCl4 group. CONCLUSION:PtPs, the effect is better than cPtPs, lessens CCl4-induced hepatonecrosis significantly. The role may be related with anti-lipid peroxidation. 相似文献
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以羊肚菌子实体为研究材料,研究了酶提羊肚菌子实体多糖的抗氧化和抗衰老活性。对比试验结果表明,羊肚菌多糖对小白鼠SOD、GSH-Px、MDA等抗氧化能力有一定的影响,对ALT、AST、BUN等血清生化指标也有影响,并且不同剂量的影响效果也有差别,但总体上都能不同程度地提高小白鼠的抗氧化和抗衰老能力,是一种理想的抗衰老保健产品,可以为羊肚菌多糖抗衰老产品的研发提供参考。 相似文献
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AIM: To investigate the effects of dexmedetomidine (DEX) on acute alcoholic hepatic injury in mice and to explore the possible mechanisms. METHODS: Kunming mice (n=50) were randomly divided into 5 groups (n=10): normal saline control (NS) group, acute alcoholic hepatic injury model (E) group, low-dose (10 μg/kg) DEX (E+L) group, medium-dose (50 μg/kg) DEX (E+M) group and high-dose (100 μg/kg) DEX (E+H) group. The animals were sacrificed at 6 h after gavage of alcohol or normal saline. The levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglyceride (TG), malondialdehyde (MDA), glutathione (GSH) and superoxide dismutase (SOD) were measured. The livers were removed for evaluation of histological characteristics and determining the content of tumor necrosis factor-α (TNF-α) amd interleukin-1β (IL-1β) in the liver tissues by ELISA. The expression levels of cytochrome P450 2E1 (CYP2E1) and nuclear factor-κB (NF-κB) in the liver tissues were evaluated by Western blot. RESULTS: Compared with NS group, the levels of ALT, AST and TG were obviously increased in E group, which were obviously decreased in E+M and E+H groups. Compared with NS group, the levels of TNF-α, IL-1β and MDA were obviously increase in E group, which were obviously decreased in E+M and E+H groups. Compared with NS group, the activity of SOD and the content of GSH were obviously decreased in E group, which were obviously increased in E+M and E+H groups. Compared with NS group, the expression of CYP2E1 and NF-κB was obviously increase in E group, which was obviously decreased in E+M and E+H groups. Compared with NS group, ethanol induced marked liver histological injury, which was less pronounced in E+M and E+H groups. CONCLUSION: DEX has a protective effect on mouse liver with acute alcoholic injury by the involvement in the processes of antioxidation and antiinflammation, and its mechanism may be associated with the inhibition of CYP2E1 and NF-κB expression. 相似文献
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AIM:To investigate the effect of Se-containing spirulina phycocyanin (Se-SPC) on liver injury of mice induced by carbon tetrachloride (CCl4). METHODS:The mouse model was conducted by intragastric feeding with 2% CCl4 oil for three times, meanwhile Se-SPC, spirulina phycocyanin (SPC) and Na2SeO3 were injected (ip) to various groups for 7 days. Then selenium (Se), glutathione peroxidase (GPx), superoxide dismutase (SOD), alanine aminotransferase (ALT), malondiaoldehyde (MDA) and nitric oxide (NO) levels in blood and liver were measured. RESULTS:The level of Se,GPx and SOD activities were obviously higher(P<0.05)but ALT activity,MDA and NO2-/NO3- levels were remarkably lower(P<0.05)in Se-SPC treated groups than those in CCl4 groups,and effects of high dose Se-SPC on Se,GPx,MDA and NO2-/NO3- were even more significant(P<0.01).Under the same dose of Se or protein,effects of all selected targets in Se-SPC groups were more efficient than those in SPC groups and inorganic-Se groups.Furthermore,Se levels had a positive correlation with GPx activity(r=01705),which had negative correlation with levels of MDA,NO2-/NO3- and ALT(r=-0.629,r=-0.336,r=-0.457,respectively), and positive correlations between ALT activity and MDA or NO2-/NO3- level were found (r=0.519,r=0.641). CONCLUSION:These results indicated that Se-SPC may attenuate liver injury of mice induced by CCl4 through its anti-inflammatory action and enhancing selenoenzyme expression. 相似文献
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AIM: To investigate the distributive rules of apoptosis index (AI) in liver with ischemia/reperfusion (I/R) injury and evaluate the factors related to the hepatocyte apoptosis. METHODS: Sixty SD rats in specific pathogen free grade were randomly divided into three groups: control group (n=18), sham operation group (n=18) and I/R group (n=24). In I/R group, liver injury was induced by blocking blood inflow in rat liver for 20 min, then reperfusion for 22 h. Rats in the control group didnt receive any management. Rats in the sham operation group only subjected sham operation. All rat blood samples and livers were obtained for determination. Blood serum ALT, AST, TBIL, TNF-α, IFN-γ, IL-4, plasma endotoxin concentration, MDA level and SOD activity in liver were detected. Hepatic histological analysis was conduced through HE staining. Apoptosis was detected by TUNEL methods. RESULTS: Focal necrosis occurred in six rats livers in I/R group, in control group and sham group no necrosis cell was found in livers. The hepatic AI of I/R group was significantly increased compared with other groups. The AI in region under hepatic amicula was higher than that in central veins region and portal area. The necrotic regions contained apoptotic cells and AI was higher than that of other regions. Hepatic AI was significantly associated with ALT, AST, TNF-α, IFN-γ and SOD/MDA. CONCLUSION: In liver with I/R injury, the apoptotic cells in the region under hepatic amicula and the focus of necrosis are significantly higher than those in other regions, apoptotic cells and necrosis cells co-exist in the same zone. Hepatic AI may be significantly associated with ALT, AST, TNF-α and SOD/MDA. 相似文献