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1.
As natural wetlands have disappeared around the world, artificial wetlands have increased. We found interesting differences in waterbird communities of two natural (Bundala Ramsar site) and seven artificial wetlands (irrigation tanks, salt ponds, rice paddies) in south-east Sri Lanka. Eight species exclusively used natural and one species artificial wetlands. Migratory species (shorebirds 64%, terns 47%) dominated species’ richness of natural lagoons, with densities of shorebirds 3-6 times greater than on artificial wetlands. Contrastingly, resident species (dabbling ducks, gallinules) contributed most to the diversity (59%) and density of waterbirds on artificial irrigation tanks. Cattle egrets dominated waterbird density (>70%) of rice paddies. Waterbird communities reflected physical and chemical character of wetlands: natural wetlands were shallow (<2 m) and saline (EC > 1000 msm−1) compared to deep (>2 m) and freshwater (EC < 110 msm−1) artificial wetlands. Artificial inputs of water drained into the natural Embilikala lagoon changing its physico-chemical profile and disrupting the natural seasonal drying. Consequently its waterbird community was similar to artificial irrigation tanks, with shorebird species particularly impacted: densities half that of the other natural lagoon. Artificial salt ponds had similar physico-chemical properties to the natural Bundala lagoon and a similar waterbird community. Even though artificial wetlands supported waterbirds, they were not adequate replacements for loss of natural wetlands, favouring some species. Imposed hydrological stability degraded habitat quality for migratory shorebirds on one of our Ramsar site lagoons.  相似文献   

2.
This investigation was conducted to determine the elemental composition of effluents from three anaerobic swine waste lagoons and one aerobic swine waste lagoon and to evaluate effects of effluent application on growth of corn (Zea mays L.) Wide variation occurred in the elemental composition of effluents among the four lagoons and among four sampling dates from each lagoon. Use of these effluents as irrigation water would lead to application of a wide range in amounts of nutrients to soil. Effluent application increased corn grain yield on a Woodstown loamy fine sand. This yield increase was attributed to alleviation of moisture stress and to correction of N deficiency.  相似文献   

3.
Semiarid lands are the object of a limited number of studies, very few among them aimed at characterizing root-associated fungal communities. The diverse vegetation occurring in the tropical dry forest from the Ceará State, Brazil, core area of the Brazilian tropical semiarid, has been attributed to its soil, topography and climatic variation. However, the arbuscular mycorrhizal (AM) symbiosis may have an important role in the function of these ecosystems. We examined AM association in 29 semiarid Brazilian species from three different locations: thorny dry woody savanna vegetation, known as caatinga; non-thorny dry forest and closed, non thorny dry tall-shrubby vegetation, known as carrasco. AM fungal diversity was also compared among the different sites. Twenty of the 22 trees and two of the seven herbs examined had AM association. Arum-type AM morphology was the dominant association occurring in 19 trees and in 3 hemicryptophyte plants. AM morphology is reported for the first time in 21 trees and two herbaceous species. Over the different sites, spore densities in the soil ranged from 5 to 32 per 100 g air-dried soil. Spores of 32 AM fungal taxa were isolated from the soil samples of trees of which twelve belonged to Acaulospora, two to Scutellospora, three to Gigaspora, four to Racocetra, three to Glomus, one to Clareoideoglomus, one to Ambispora, one to Pacispora, one to Sclerocystis, one to Dentiscutata, one to Orbispora, one to Quatunica and one to Entrophospora. Species richness was high in woody caatinga and Glomus macrocarpum, Gigaspora gigantea and Cetraspora pellucida were the most frequent species at different sites. Species diversity (Shannon–Weaver index) did not differ significantly among sites. Water content and phosphorus availability was found to influence the AMF species composition at the plant community level, providing information about the caatinga dominium biodiversity, mainly for its conservation.  相似文献   

4.
The concentrations of Pb, Cd, Cr, Mn and Fe were evaluated in leaves, stem and root of the Scirpus americanus and Typha latifolia aquatic macrophytes, which were collected from Tanque Tenorio, an artificial lagoon highly polluted by municipal and industrial wastewater. Some S. americanus and T. latifolia plants were collected from four different sites within Tanque Tenorio. The sites were chosen regarding their proximity with the main channel discharging wastewater into the lagoon. The results showed that S. americanus and T. latifolia have the ability to extract Pb, Cd, Cr, Mn and Fe from their water surroundings; on the whole, the roots presented higher concentrations of heavy metals than the stem and the leaves. The highest accumulation of heavy metals was observed in plants growing at the site near the channel entering the lagoon. S. americanus accumulated more Pb, Cr, Mn and Fe than T. latifolia; Cd concentrations were comparably the same in both species. This study provides information in relation to aquatic plants growing in polluted waters, which accumulate heavy metals. These findings are of interest pertaining to the removal processes for treating aquatic systems with heavy metal content.  相似文献   

5.

Purpose

To characterise soil humic acids (HAs) extracted from Spanish marshes formed under different vegetation types (Spartina maritima (GSp), Juncus maritimus (GJc), Phragmites australis (GPh), and Scirpus maritimus (VSc)), soil depths (0–20, 20–40 and 40–60 cm), physiographic position (low and high marshes), wetland types (salt marshes and lagoons) and environmental conditions (Atlantic and Mediterranean coast).

Material and methods

Soil samples were collected in five Spanish marshes, three on the Galicia province and two on the Valencia province. Humic acids were extracted and their elemental composition, semiquinone-type free radical (SFR) content, FTIR and CPMAS 13C NMR spectra determined. Total carbon (TC), total nitrogen (TN), total sulphur (TS), CaCO3 content, and field pH and Eh (mV) in the marsh soils sampled were also measured.

Results and discussion

The field pH and Eh values were typical of coastal areas submitted to periodic inundations and the highest TC, TN and TS contents were found in the soil of lagoon marshes as an effect of physiographic position and wetland type. The HAs, in general, were highly aliphatic and exhibited a low SFR content, which suggests a low humification degree of the SOM formed in the studied areas. This is a result of the anaerobic decomposition to which SOM is submitted and the high input of plant-derived organic matter (OM) by vegetation. However, among the studied sites low salt marsh and subsurface layer of the high salt marsh showed higher SFR content, simpler FTIR spectra, higher lignin degradation and lower O-alkyl C/alkyl C ratio than the lagoon marshes, thus suggesting the presence of a more humificated SOM in these sites.

Conclusions

From the different factors analysed, only physiographic position (low versus high salt marshes) and wetland type (marshes versus lagoons) caused variations in the HAs characteristics, because as the studied soils are under anaerobic conditions, they control the exportation of plant-derived OM and the allochthonous OM contribution in the studied areas.  相似文献   

6.
Altitudinal gradients are useful to study the potential effects of climate change on ecosystems. Historically, studies on elevation gradients have primarily focused on macro-organisms and ecosystem processes, while microorganisms have been mostly ignored despite their ubiquity and functional importance. We studied the temporal (about every two months from June 2008 until May 2009) variation of testate amoeba communities in four Sphagnum-dominated peatlands along a 1300 m elevation gradient in the Swiss Mountains (580–1880 m) in relation to water table depth and hydrochemistry with special focus on dissolved organic carbon (DOC), a useful proxy for changes in C-cycling in peatlands.The lowest site had significantly (P < 0.01) lowest testate amoeba density, species richness. The highest site had highest testate amoeba density (38 ind mg−1 dry mass of Sphagnum). Seasonal fluctuations in testate amoeba species richness and diversity were not consistent among sites but density tended to peak in spring at all sites, autumn in the three highest sites and mid-winter in the upper two sites. In a redundancy analysis (RDA) community structure was more strongly correlated to altitude (33.8% of variance explained in living community) than to soil hydrological and hydro-chemical variables (together explaining 16.2% of variance). In a partial RDA with altitude used as covariable, the four sites were separated by DOP, DOC, DON, pH and average depth to water table.The abundance of high trophic level testate amoeba species (shell-aperture over their body size >0.20; i.e. primarily predators of protists and micro-metazoa) as well as the community size structure increased from lowest to highest elevation (respectively by 3.7× and 6×) and followed the seasonal patterns of total density, while DOC, DON, and DOC/DON decreased with elevation.These results agree with previously reported alteration of peatland microbial food chains in response to experimental warming, suggesting that climate-induced changes in microbial community structure (here a shortening of microbial food chains) represent a mechanism controlling the carbon balance of peatlands.  相似文献   

7.
Diatoms are good indicators of water quality in lotic systems. Unlike in the temperate region, the effect of substrate on diatom-based water quality assessment in tropical streams is not fully understood. The purpose of this study was to assess the effect of substrate on diatom-based multivariate water quality assessment. Epilithic, epiphytic, epipsammic and epipelic diatom community and water quality sampling was done four times at 10 sites during the dry season (2008 and 2009). Artificial substrates (brick and glasses) were also placed at the sampling sites during this period and sampled after 1 month. Cluster analysis was performed to show the main differences and similarities in community composition amongst substrates sampled and amongst sampling sites. The IndVal method was used to identify indicator species characterising different substrates. Canonical correspondence analyses (CCAs) were performed to relate the structure of diatom communities from different substrates to predictor variables. A gradient of increasing metal and organic pollution, eutrophication and ionic strength was observed from the agricultural/forested area to the urban area. Diatom community structure closely reflected this gradient, with communities from polluted sites (8, 9 and 10) being different from other communities. Polluted sites were associated with such species as Nitzschia palea, Plantago lanceolata, Achnanthes exigua, Caloneis hyaline, Cyclotella meneghiniana, Gomphonema parvulum, Fallacia monoculata, Luticola goeppertiana, Pinnularia microstauron, Pinnularia subcapitata and Sellaphora pupula. Indicator species analysis showed that common diatom species were not restricted to a single substrate, though preference was generally high for natural (especially macrophytes) compared to artificial substrates. Six CCAs corresponding to six substrates performed to relate diatom community structure to simultaneous effects of predictor variables explained ~50% of the diatom species variance in all cases and roughly separated highly polluted sites from the rest of the sites. This indicates that the results of diatom-based multivariate water quality assessment based on different substrates may be interchangeable. Only one substrate has to be collected at each site for water quality assessment surveys, thus avoiding unnecessary expensive and time-consuming oversampling. Given the limitations of artificial substrates, sampling of natural substrates is highly recommended.  相似文献   

8.
《Applied soil ecology》2011,47(3):443-449
Pontoscolex corethrurus (Glossoscolecidae, Oligochaeta) is an invasive endogeic earthworm that has colonized most land transformed by human activities in the humid tropics. When installed, populations can change soil physical properties, biogeochemical processes and microbial communities. The aim of this study was to determine whether P. corethrurus establishment is a result of (1) a competitive exclusion of native earthworm species or (2) the exploitation of a new niche created by anthropogenic disturbance that native earthworm species cannot use. We tested these hypotheses by doing a survey of earthworm communities in 270 sites that represented the diversity of land use systems encountered in two contrasted regions of the Amazonian arc of deforestation located in Brazil and Colombia respectively. When present in forests, P. corethrurus had no negative effect on the native species communities that had similar (epigeic species) or even higher densities (endogeic species) in the presence of the invasive species. These results suggest the absence of competitive exclusion.The first two axes of a PCA multivariate analysis of communities represented the densities of native species (axis 1) and P. corethrurus (axis 2) respectively. This suggests that respective densities of the two groups respond to different conditions and that their variations are independent. The density of P. corethrurus co-varied with soil N content and pH in Colombian sites while the densities of other species did not. Our results thus suggest that this invasive species, unlike native species, is able to feed and develop in environments where litter resources are decreased while soils have been enriched in C and nutrients by deforestation and burning. We discuss the reasons why some primary forests in Central America have large populations of P. corethrurus.  相似文献   

9.
Shifting sand dunes threatened the Baotou-Lanzhou railway being kept on operation smoothly seriously crossing Shapotou on the southeastern edge of the Tengger Desert (China). Artificial vegetation input was employed in Shapotou and a vegetated belt was established for stabilizing the shifting sand dunes. Nematode communities from bare soil between vegetation (ST, STI) and soil under the vegetation (ST-V, STI-V) in the vegetation belt, were investigated. All sites received natural rainfall, of which STI and STI-V received irrigation supplements. Total 43 genera were found in four types of soil samples, and Acrobeles, Acrobeloides, Chiloplacus, Panagrolaimus, Aphelenchoides and Ditylenchus were dominant genera in our study. Nematode abundance per 100 g fresh soil (47–552), the number of taxa identified (8–20), species richness (1.51–3.41), the proportion of plant feeders (0.3%–4.2%) and abundance of all cp groups responded to vegetation. The proportion of omnivores (0.5%–7%), the number of taxa identified, Shannon index (1.26–2.12), species richness, genus dominance (0.18–0.40) and abundance of cp3–5 responded to irrigation supplements. The application of PCA helped to reveal that almost all nematode taxa exhibited a positive loading on the horizontal axis, it implied that nematode abundance was generally higher on sites with planted input. Besides, it showed a vegetation gradient from bare soil between vegetation to soil under vegetation along the first axis, while an irrigation gradient along the second axis. Similarly, classification analysis based on all cased involved indicated that two-main group of nematodes was distinguished by their habitats under vegetation from bare soil. Those results suggested that vegetation input might play a more important role on nematode community composition than irrigation supplements for stabilization of shifting sand dunes. Therefore, nematode community composition could be as an indicator of stabilization of sand dune approaches of plant input with only natural rainfall versus with natural rainfall in combination with irrigation supplements in our system. However, the apparent anhydrobiotic state of the nematode fauna required special consideration in desert system, thus the utilization of the nematodes as an indicator of stabilization of sand dune regimes should be further explored on anhydrobiotic nematodes.  相似文献   

10.
Exceptionally high concentrations of Cd (100 to 200 ppm dry weight) were found in samples of Halobates collected off Baja California. In contrast, no detectable Cd was found in Rheumatobates collected from mangrove lagoons in the Gulf of California. Concentrations of five other heavy metals in the two marine insects are also presented.  相似文献   

11.
A protozoological survey was performed on waste stabilization lagoons to isolate, identify, and determine the incidence of zooflagellates present in these waters. A correlation between the species found and the physicochemical parameters determined was also done. Fifteen samplings were analyzed from June 1981 to April 1982. Free-living zooflagellates included: Bodo edax Klebs: Bodo caudatus Dujardin;Trepomonas agilis Dujardin;Pleuromonas jaculans Perty (all of them polisaprobic indicators);Bodo saltans Ehrenberg (a mesosaprobic indicator); and Bodomorpha minima Hollande. A human commensal species Enteromonas hominis Da — Fonseca, was also isolated. The performance of the lagoons was predominantly anaerobic. The temperature seemed to be the most influential factor on the incidence of the zooflagellates isolated, especially in winter time.  相似文献   

12.
Little is known about the characteristics of arbuscular mycorrhizal fungi (AMF) community in the roots of host plants growing on heavy metal contaminated sites. The objectives of this study were to examine the community structure of AMF associated with the roots of a copper (Cu) tolerant plant—Elsholtzia splendens in a Cu mining area in southeastern Anhui Province, China. Molecular techniques were used to analyze AMF community composition and phylogenetic relationship in E. splendens roots sampled from three Cu mine spoils and two adjacent reference areas. Results obtained showed that root colonization and AMF diversity were very low and negatively correlated with total and extractable Cu concentrations. All the DNA sequences recovered belonged to the genus of Glomus. The principal component analysis (PCA) revealed that the AMF community composition varied remarkably among different sites and was related closely to soil properties, especially Cu concentrations. The distribution pattern of AMF species in various sites suggested the degree of AMF tolerance to Cu contamination. The unique AMF species that presented exclusively in heavily contaminated sites need to be further examined for potential application in phytoremediation of metal contaminated soils.  相似文献   

13.
14.
Resurveys of plant communities provide valuable information on changes in species composition over time and clues about how species respond to environmental change. We report results from resurveys of 62 upland forest stands in northern Wisconsin and the western Upper Peninsula of Michigan first surveyed around 1950. We identify plant species that have significantly increased or decreased in frequency in 1 m2 quadrats (‘winners’ and ‘losers’) and evaluate the traits that distinguish these groups. Twenty-one winner species increased across sites (by 25-400%), while 21 loser species decreased (by 21-95%). Winners include both common, native species and five invading exotics. Many are grasses or sedges and most are abiotically pollinated or dispersed (e.g., Carex, which increased 286%). Losers are mostly rarer native forbs that rely on animals for pollination and/or dispersal. Losers appear sensitive to desiccation, anthropogenic disturbance, and/or herbivory by white-tailed deer (e.g., Streptopus roseus, which decreased 73%). Declines in loser species are heterogeneous and stochastic across sites whereas increases in winners are more uniform and deterministic. Increases in common widespread native species account for most of the community change we observe across sites. The fact that winners resist or tolerate deer herbivory while many losers are sensitive to deer suggests that deer may be a key driver of the shifts we observe in these forests.  相似文献   

15.
The effectiveness of reduced-impact logging practices on the maintenance of biodiversity in Borneo has been recognized for some organisms (e.g., mammals). We investigated the effects of reduced-impact logging and conventional selective logging practices on biodiversity by using soil fauna as indicators of disturbance. The study sites were the production forests of the Deramakot Forest Reserve and the Tangkulap Forest Reserve in Sabah, Malaysian Borneo (5°14–30′ N, 117°11–36′ E). We compared macro- and mesofauna in a pristine forest with no logging, a reduced-impact logged forest in Deramakot Forest Reserve, and a conventionally logged forest in Tangkulap Forest Reserve.The mean density of soil macrofauna (excluding ants) did not differ significantly among the three forest categories (nested ANOVA, p > 0.05). This tendency was also seen in the density and species richness of Oribatida and Collembola, which showed little difference among forest categories. Nonmetrical multidimensional scaling (NMS) ordination diagrams revealed a different community composition in conventionally logged forest sites compared with the other sites. The scores for the NMS first or second axis of soil fauna were correlated with one or more of the values for density, diversity, and species composition of trees. A RELATE test showed the congruence between trees and the Collembola and Oribatida community composition between sites. The results implied that the soil fauna community composition was related to tree communities. In conclusion, the impacts of logging on decomposers in the soil animal communities have been mitigated by the introduction of reduced-impact logging in Deramakot Forest Reserve through the protection of tree vegetation. It is important to consider monitoring the influence of selective logging on soil fauna with regard to the dynamics of the species (or group) composition because total density and species (or group) richness of soil fauna displayed only a marginal response to the different logging practices.  相似文献   

16.
In a Japanese forest, CH4 uptake rate and methanotrophic community structure in the soil were investigated at four sites of different vegetation. At two of these sites, undergrowth was dominated by Sasa senanensis, and that of another was dominated by Sasa kurilensis. At the rest site, undergrowth had been removed artificially. The tree-layer composition differed between the two sites with S. senanensis, but tree layer of the other two sites were dominated by the same species. At the site lacking undergrowth, observed CH4 uptake rate was twice as high as that at the other sites. Under laboratory conditions, soil sample from the site lacking undergrowth exhibited CH4 consumption rate higher than that of the adjacent site with the same dominating tree species. The community structures of methanotrophs were investigated with denaturing gradient gel electrophoresis (DGGE) of the gene encoding particulate methane monooxygenase (pmoA). The banding patterns observed were different depending on the type of undergrowth vegetation. The sequences of the DGGE bands were closely related to each other and belonged to the “upland soil cluster alpha” (USCα). These results imply possible close relationship between the undergrowth vegetation and methanotrophic communities in forest soils.  相似文献   

17.
Estuarine sediments are the repository for a wide range of contaminants. Anthropogenic impacts and variations in the belowground biomass of salt marsh plants potentially select for different sediment microbial communities with different functional capabilities, including the ability to biotransform anthropogenic contaminants. There are large differences in both root morphology and the amount of fine root biomass of Spartina alterniflora and Phragmites australis; Spartina is the species commonly used to replace Phragmites in northeastern US salt marsh restoration projects. Our study compared the effect of these two macrophyte species on sediment microbial communities responsible for the biotransformation of the halogenated flame retardant tetrabromobisphenol A (TBBPA). Sediments were obtained from contaminated and uncontaminated salt marsh field sites in New Jersey. Anaerobic methanogenic sediment microcosms were established and incubated for up to 130 days. TBBPA was reductively dehalogenated resulting in the transient formation of two intermediates, identified as tribromobisphenol A and dibromobisphenol A, and the formation and accumulation of bisphenol A (BPA) as the end product. Spartina sediments from both sites were found to dehalogenate TBBPA more rapidly than the Phragmites or unvegetated sediments, resulting in greater production of BPA. Microbial community diversity as measured by in situ sediment phospholipid fatty acid (PLFA) composition prior to TBBPA exposure, was found to be higher in the uncontaminated sediments; differences in microbial PLFA diversity were not seen in contaminated sediments associated with either the different plant species or unvegetated sediment. The results of this study demonstrate that these two plant species affected sediment microbial community function with respect to dehalogenation capabilities, even though the disturbed and undisturbed sediments varied in microbial community composition.  相似文献   

18.
Across large areas of upland Britain Molinia caerulea and Nardus stricta are replacing Calluna vulgaris as the dominant plant species of the vegetation assemblage. Associated changes in the composition of the whole plant assemblage and a phytophagous insect group, the Hemiptera, were investigated. Vegetation and hemipteran assemblages were sampled at six moorland sites in northern England and Scotland. Sampling at each site was carried out in Calluna vulgaris-dominated areas and grass-dominated areas that had previously been dominated by C. vulgaris. Vegetation assemblages of heath and grass sites differed significantly even when species by which sites were selected for sampling (C. vulgaris, M. caerulea and N. stricta) were removed from the analysis with the highest species richness in dry grassland sites. Hemipteran assemblages differed significantly between heath and grass sites with greater species richness also being in grass sites. Plant species composition was the most important explanatory variable of hemipteran assemblages with temperature, latitude, soil loss on ignition and vegetation height also explaining a significant proportion of the variation. Overall a range of vegetation composition and structural variables explained 40.8% of variation in the Hemiptera species data with ranges of soil and biogeographic variables explaining an additional 8.7%.This research demonstrates that the replacement of one dominant plant species by another is associated with a change in other aspects of moorland biodiversity. In particular the loss of C. vulgaris actually leads to an increase in the species richness of Hemiptera and plants although the number of specialist species declines.  相似文献   

19.

Purpose

Concentrations and transformations of mercury were measured in river, estuarine, and marine sediments to determine factors affecting the fate of mercury entering the northern Adriatic Sea.

Materials and methods

Radiotracer methodology was used to compare rates of mercury methylation (203Hg), MeHg demethylation (14C), and sulfate reduction (35S) in sediment depth profiles to concentrations of total and dissolved mercury species in the lower freshwater region of the Isonzo River, the coastal lagoons, and in the Gulf of Trieste, northern Adriatic Sea.

Results and discussion

Mercury was readily methylated and demethylated in all sediments, but the relative activity of these processes varied greatly with location. Methylation activity increased greatly from freshwater to the marine regions; however, demethylation was extremely high in the estuarine and lagoon sites. Ratios of methylation to demethylation were low in these coastal sites but increased further offshore in the gulf, which agreed with increased ratios of MeHg to total Hg (%MeHg) in gulf sediments. Comparisons of microbial activities indicated that sulfate reduction strongly controlled both methylation and demethylation. However, Hg methylation in coastal lagoon sediments was controlled by rapid demethylation and the bioavailability of Hg that was affected by Hg adsorption and precipitation. Methylation in offshore marine sites correlated with sulfate reduction but not the partitioning of Hg between pore water and solid phases. The decrease in sulfide production offshore exacerbated Hg methylation.

Conclusions

The freshwater to marine gradient in the Idrija/So?a/Isonzo/Adriatic region is dynamic, exhibiting horizontally variable rates of microbial activities and Hg transformations that create “hot spots” of MeHg accumulation that are controlled differently in each region.
  相似文献   

20.
The montado is an agro-silvo pastoral system characterized by an open oak formation combined with shrubs or a rotation of cultures/pastures/fallow. A key, but frequently overlooked, component of these systems is the fungal community associated with the dominant oaks. We present a study of the basidiomycete community in montado areas of Quercus suber L. from Central-Western Portugal, based on fruiting body assessment and PCR-DGGE of soil mycelium. Soil available phosphorus and organic matter content were also determined. The most frequent fruiting body found in the cork oak montados belonged to Lactarius, Clitocybe, Russula and Cortinarius species. Lactarius chrysorrheus was the most widely distributed species. Soil management practices cause a temporal increase in soil available phosphorus, and harrowing and fertilizer application also result in an increase in soil organic matter content. Mechanical clearing with recent soil disturbance had a negative effect on the richness and diversity of the basidiomycete community assessed using fruiting body. The molecular analysis of the basidiomycete community separated the sites with recent shrub-removal from the others sites. Our results show that soil management techniques that avoid disturbance of the top-soil layers are the best way to preserve the structure and diversity of the soil fungal community in the montado.  相似文献   

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