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1.
It has been suggested by others that the size of the flush of mineralization caused by CHC13 fumigation can be used to estimate the amount of microbial biomass in soils. Calculation of biomass from the flush requires that the proportion of CHCl3-killed cell C mineralized be known. To determine this proportion, 15 species of [14C]labelled fungi and 12 species of [14C]labelled bacteria were added to four types of soil and these were fumigated for 24 h with CHC13, reinoculated with unfumigated soil, and incubated at 22°C for 10 days. The average percentage mineralization of the fungi was 43.7 ± 5.3, while the average for the bacteria was 33.3 ± 9.9. Using a 1:3 ratio for distribution of total biomass between the bacterial and fungal populations, respectively, it was calculated that the average mineralization of both types of cells was 41.1%. In experiments conducted to determine if CHC13 vapour alters stabilized microbial metabolites or dead microbial cells in a manner which makes them more susceptible to degradation, it was found that both fumigated and unfumigated dead fungal materials mineralized to the same extent in soil during 10 days of incubation.  相似文献   

2.
We analysed the decomposition of 14C-labelled straw at five different levels of heavy metal contamination (100-20,000 µg total Zn g-1 soil) in non-fumigated and repeatedly fumigated soils. The soils were not spiked with Zn, but were taken from sites containing different heavy metal concentrations. Zn was only used as a reference and the effects observed are most likely due to this metal. Microbial biomass decreased with increasing heavy metal content of soils, paralleled generally by the decreasing amount of wheat straw 14C incorporated into the microbial biomass. In addition, the newly synthesised microbial biomass declined more rapidly as the incubation proceeded. In the repeatedly fumigated soils, microbial biomass 14C corresponded to roughly 50% of the maximum 14C incorporation of the non-fumigated soil. The relative decline during incubation was similar to that of the non-fumigated soil at the respective contamination level. These results reveal clearly that heavy metal effects on straw decomposition do not depend on the ratio of substrate C to microbial biomass C. In contrast to microbial biomass C, the mineralisation of the wheat straw was not seriously affected by heavy metal contamination. The same was true for all of the repeatedly fumigated treatments, where a much smaller microbial biomass mineralised nearly the same amount of straw as in the non-fumigated soils. However, repeated fumigation caused a strong reduction in the decomposition of soil organic matter. The ratio of CO2-14C to microbial biomass 14C after 60 days was linearly related to the Zn concentration in both non-fumigated and repeatedly fumigated samples, clearly indicating that an additional energy cost is required by soil microorganisms with increasing heavy metal concentrations.  相似文献   

3.
Soil microbial biomass has been determined since the mid 1970's by the chloroform fumigation incubation technique as proposed by Jenkinson and Powlson (1976). The microbial biomass C can be determined by subtracting the CO2 emitted from an unfumigated soil (mineralization of soil organic matter) from that emitted from a chloroform fumigated inoculated soil (mineralization of soil organic matter and killed soil microorganisms) and dividing the difference by a proportionality factor (kC = 0.45). The question remained which microorganisms recolonized a fumigated soil. An arable soil was fumigated for one day with ethanol-free chloroform or left unfumigated and incubated aerobically after removal of the chloroform for 10 days. The bacterial population structures were determined in the fumigated and unfumigated soil after 0, 1, 5 and 10 days by means of 454 pyrosequencing of the 16S rRNA gene. Fumigating the arable soil reduced significantly the relative abundance of phylotypes belonging to different groups, but increased the relative abundance of only four genera belonging to two phyla (Actinobacteria and Firmicutes) and two orders (Actinomycetales and Bacillales). The relative abundance of phylotypes belonging to the Micromonospora (Micromonosporaceae) increased significantly from 0.2% in the unfumigated soil to 6.7% in the fumigated soil and that of Bacillus (Bacillaceae) from 3.6% to 40.8%, Cohnella (Paenibacillaceae) from undetectable amounts to 0.6% and Paenibacillus (Paenibacillaceae) from 0.3% to 4.2%. The relative percentage of phylotypes belonging to the Acidobacteria, Bacteroidetes, Chloroflexi, Gemmatimonadetes and Proteobacteria (α- β-, δ- and γ-Proteobacteria) were significantly lower in the fumigated than in the unfumigated soil and in most of them the relative abundance of different bacterial orders (i.e. Gp3, Gp4, Gp6, Sphingobacteriales, Gemmatimonadales, Rhodospirillales, Burkholderiales, Xanthomonadales) was reduced strongly (P < 0.001). It was found that the relative abundance of a wide range of bacteria was reduced shortly after fumigating an arable soil, but only a limited group of bacteria increased in a fumigated arable soil indicating a capacity to metabolize the killed soil microorganisms or recolonize a fumigated soil.  相似文献   

4.
In a previous paper (1), a modification of the chloroform fumigation method (2) was proposed for estimating microbial biomass-nitrogen (N) in submerged soil. It was found that a large part of the living bacteria in the submerged soil was killed within about 12 h of the fumigation and was actively mineralized by subsequent anaerobic incubation.

In this paper, the following factors which influence the estimated amount of microbial biomass-N were examined: a) inoculation to fumigated soil, b) duration of anaerobic incubation, c) storage and sieving pretreatments of soil, and d) effect of fumigation on soil organic matter.  相似文献   

5.
The influence of the composition of added organic C on microbial functional diversity was investigated in an arable soil. Soil was treated with single large additions of organic C compounds (asparagine, gluconic acid, glutamic acid, mannose, methyl–glucamine and urocanic acid) or repeated additions of compounds (asparagine, glucosamine, -ketovaleric acid or urocanic acid) over 4 d. The effects on microbial functional diversity were assessed by measurement of patterns of in situ catabolic potential (ISCP) in each experiment at 1, 7 and 20 weeks, and 1, 4 and 7 d, respectively. Microbial biomass C was generally increased by the substrate additions in both experiments and remained greater than the control over 7 d in the repeated-addition experiment. After 20 weeks, however, only the microbial biomass C in the asparagine and mannose treatments remained increased in the single-addition experiment. The direction of changes in the patterns of ISCP were distinctly different between most treatments in both the single- and repeated-substrate addition experiments. Over 20 weeks, factor analysis showed that the patterns of ISCP in the glutamic acid, mannose, methyl–glucamine and urocanic acid treatments tended to converge with that in the non-amended treatment. The changes in patterns of ISCP were also different after repeated additions of different substrates. However, respiration responses to the added substrate in each of the treatments were not specifically increased relative to the respiration responses to other organic C compounds. These results support the hypothesis that addition of different types of organic C can influence patterns of ISCP. However, it is difficult to distinguish the direct effects of the added compounds on the respiration response to individual substrates.  相似文献   

6.
Early season problems with growth of corn (Zea mays L.) under cool, wet conditions prompted a study of the effects of soil and environmental conditions on mineralization and plant uptake of phosphorus (P). Our objective was to determine the effect of soil test P, temperature, and soil fumigation on soil P availability and uptake during early corn growth. Corn was grown in growth chambers at temperatures of 14°C or 25°C. Soils were a high‐P Hastings silty clay loam (fine, montmorillonitic, mesic Udic Argiustoll) and a low‐P Sharpsburg clay loam (fine, montmorillonitic, mesic Typic Argiudoll). Plants grew for up to 42 d either in soil which had been fumigated with methyl bromide to reduce microbial populations or left unfumigated. We harvested whole pots for soil and plant analysis at 1, 14, 28, and 42 d after planting. Biomass carbon (C) and biomass P were lower in fumigated soils and biomass C increased with time. Fumigation increased Bray Pl‐extractable P at all times. Phosphatase activity and mycorrhizal colonization were both reduced by fumigation. Cumulative plant P uptake was highest in Hastings at 25°C. Higher temperature and higher initial P status increased plant P uptake during early growth. Plants grown in fumigated soil did not take up more P, despite greater extractable P.  相似文献   

7.
An 8-yr (1998–2005) field experiment was conducted on a Gray Luvisol (Boralf) soil near Star City, Saskatchewan, Canada, to determine the effects of tillage (no-tillage – NT and conventional tillage – CT), straw management (straw retained – R and straw not retained – NR) and N fertilizer (0, 40, 80 and 120 kg N ha−1, except no N to pea (Pisum sativum L.) phase of the rotation) on seed and straw yield, mass of N and C in crop, organic C and N, inorganic N and aggregation in soil, and nitrous oxide (N2O) emissions for a second 4-yr rotation cycle (2002–2005). The plots were seeded to barley (Hordeum vulgare L.) in 2002, pea in 2003, wheat (Triticum aestivum L.) in 2004 and canola (Brassica napus L.) in 2005. Seed, straw and chaff yield, root mass, and mass of N and C in crop increased with increasing N rate for barley in 2002, wheat in 2004 and canola in 2005. No-till produced greater seed (by 51%), straw (23%) and chaff (13%) yield of barley than CT in 2002, but seed yield for wheat in 2004, and seed and straw yield for canola in 2005 were greater under CT than NT. Straw retention increased seed (by 62%), straw (by 43%) and chaff (by 12%) yield, and root mass (by 11%) compared to straw removal for barley in 2002, wheat in 2004, and seed and straw yield for pea in 2003. No-till resulted in greater mass of N in seed, and mass of C in seed, straw, chaff and root than CT for barley in 2002, but mass of N and C were greater under CT than NT for wheat in 2004 and for canola in 2005 in many cases. Straw retention had greater mass of N and C in seed, straw, chaff and root in most cases compared to straw removal for barley in 2002, pea in 2003 and wheat in 2004. Soil moisture content in spring was higher under NT than CT and with R than NR in the 0–15 cm depth, with the highest moisture content in the NT + R treatment in many cases. After eight crop seasons, tillage and straw management had no effect on total organic C (TOC) and N (TON) in the 0–15 cm soil, but light fraction organic C (LFOC) and N (LFON), respectively, were greater by 1.275 Mg C ha−1 and 0.031 Mg N ha−1 with R than NR, and also greater by 0.563 Mg C ha−1 and 0.044 Mg N ha−1 under NT than CT. There was no effect of tillage, straw and N fertilization on the NH4-N in soil in most cases, but R treatment had higher NO3-N concentration in the 0–15 cm soil than NR. The NO3-N concentration in the 0–15, 15–30 and 30–60 cm soil layers increased (though small) with increasing N rate. The R treatment had 6.7% lower proportion of fine (<0.83 mm diameter) and 8.6% greater proportion of large (>38.0 mm) dry aggregates, and 4.5 mm larger mean weight diameter (MWD) compared to NR treatment. This suggests a lower potential for soil erosion when crop residues are retained. There was no beneficial effect of elimination of tillage on soil aggregation. The amount of N lost as N2O was higher from N-fertilized (580 g N ha−1) than from zero-N (155 g N ha−1) plots, and also higher in CT (398 g N ha−1) than NT (340 g N ha−1) in some cases. In conclusion, retaining crop residues along with no-tillage improved some soil properties and may also be better for the environment and the sustainability of high crop production. Nitrogen fertilization improved crop production and some soil quality attributes, but also increased the potential for NO3-N leaching and N2O-N emissions, especially when applied in excess of crop requirements.  相似文献   

8.
Knowledge concerning the location of soil enzymes and their kinetics is necessary for a better understanding of their roles in biogeochemical cycles. A rapid dichloromethane fumigation procedure and a new extraction method giving negligible co-extraction of humic substances were used. Fumigation and consequent cell lysis increased the activity of arylsulfatase, but not that of acid and alkaline phosphomonoesterase, suggesting that only the former enzyme was located both extracellularly and intracellularly. Extracted arylsulfatase displayed a Michaelis–Menten constant (Km) significantly lower than in fumigated soil, which in turn was significantly lower than in unfumigated soil. In contrast, each phosphomonoesterase displayed the same Km in extracts, fumigated and unfumigated soils. These results show that activity of soil phosphomonoesterases is not influenced by any direct interaction with soil and, therefore, are more likely to be extracellular enzymes primarily devoted to biogeochemical cycling than are arylsulfatases.  相似文献   

9.
Earthworm burrows contribute to soil macroporosity and support diverse microbial communities. It is not well known how fluctuations in soil temperature and moisture affect the burrowing activities of earthworms. The objective of this experiment was to evaluate the maximum depth and length of burrows created by the endogeic earthworm Aporrectodea caliginosa (Savigny) and the anecic earthworm Lumbricus terrestris L. for a range of temperatures (5–20 °C) and soil water potentials (−5 and −11 kPa). The laboratory microcosm was a plexiglass chamber (45 cm high, 45 cm wide) containing 0.14 m2 of pre-moistened soil and litter, designed to house a single earthworm for 7 days. Earthworm mass, surface casting and burrowing activities were affected significantly by soil temperature, moisture and the temperature×moisture interaction. Burrow length and maximum burrow depth increased with increasing temperature, but there was less burrowing in wetter soil (−5 kPa) than drier soil (−11 kPa). Weight gain and surface casting, however, were greater in soil at −5 kPa than −11 kPa. Our results suggest more intensive feeding and limited burrowing in wetter soil than drier soil. Earthworms inhabiting the non-compacted, drier soil may have pushed aside particles without ingesting them to create burrows. The result was that earthworms explored a larger volume of soil, deeper in the chamber, when the soil was drier. How these burrowing activities may affect the community structure and activity of soil microorganisms and microfauna in the drilosphere remains to be determined.  相似文献   

10.
Cycles of soil drying followed by rewetting occur in most terrestrial ecosystems, but there is conflicting evidence as to the role of osmolytes in dry–wet cycles. The broad aim of this experiment was to determine how N-containing osmolytes and other organic N monomers are affected by rewetting of a moderately dry soil. In a sub-alpine grassland, experimental plots were irrigated with 50 mm of water near the conclusion of a typical late-summer drying cycle. Twelve putative osmolytes (proline, 8 quaternary ammonium compounds, trimethylamine N-oxide, ectoine, hydroxyectoine) and 60 other organic N monomers were identified and quantified by capillary electrophoresis-mass spectrometry of the free/exchangeable pool of soil water (0.5 M K2SO4 extracts) and microbial biomass (via chloroform fumigation extraction). The total concentration of organic N monomers was 25-times greater in fumigated than unfumigated extracts. Differences in relative abundance of compound classes and compounds between fumigated and unfumigated extracts suggested some compounds were localized to the free/exchangeable pool; others were predominantly microbial, whereas many were shared between pools. A striking feature of the free/exchangeable pool was that on an N-basis alkylamines were the most abundant compound class and accounted for 34% of the pool of organic N monomers. There was no evidence that osmolytes were the primary means soil microbes coped with dry–wet cycles. Instead, the pool of osmolytes was an invariant 4% of the pool of CE-MS detected monomers in K2SO4 extracts and 7% of the pool of CE-MS detected monomers in the chloroform-labile (microbial) fraction. The absence of substantial amounts of osmolytes may be because water stress was too mild or brief, or because osmolyte synthesis was limited by availability of energy, N or C and some alternative strategy was used to cope with water deficits.  相似文献   

11.
Four cultural amendments; spent mushroom compost, straw mulch, both compost and straw mulch, or neither, were applied to soils that were either fumigated or not fumigated in a field of potatoes subject to early dying and Colorado potato beetle defoliation. Two plant samples were harvested at two week intervals to measure shoot and tuber growth and mineral nutrition, and two rows were harvested for yield at maturity. Amending the soil with compost increased vegetative growth and shoot weight more than final yield of tubers. Compost amendment delayed tuber filling by several days. Fumigation partly controlled the loss of leaf area due to early dying, but it did not increase tuber yields, and in 1994 fumigation reduced tuber yield in compost amended soils. The effects of compost and straw mulch on tuber yield were related to the concentrations of N and P in leaves. The potato crop did not benefit from compost amendment combined with fumigation, because in fumigated soil there was no improvement in plant nutrition due to compost.  相似文献   

12.
The unique capacity of fungi to efficiently sequester carbon in aerobic conditions, presents a way to maximize OC gain in agricultural systems. Oat (Avena sativa) was planted in the temperate climate of southern Ontario, Canada to study factors affecting soil organic carbon (OC). The plots varied with initial OC from 25 to 68 g kg−1 or with ground cover of differing decomposability (alfalfa (Medicago sativa) growing from seed, dried oat straw, dried hay and compost) on high OC soil (60–70 g kg−1). The soil was analysed for correlation of changes in soil aggregation, moisture, OC, fungal hyphal number and length and distribution of organic matter by mass and OC in density fractions within the growing season. At harvest, soil OC and moisture were increased only in plots with ground cover. Total hyphal length was not significantly different with ground cover treatment at harvest, and did not correlate with soil aggregation and soil OC. However, the number of hyphae with >5 μm diameter (primarily mycorrhizal fungi) correlated with % OC in ground cover plots while the number of hyphae <5 μm (primarily saprophytic fungi) correlated with % OC without ground cover in the gradient of initial soil OC. Mycorrhizal hyphae may be important to the increases in soil OC from surface treatment, although there was no treatment effect of mycorrhizal occurrence on the oat roots. This microcosm study, with growing and dried ground cover, suggests surface management may a simple and inexpensive means in agriculture to increase soil moisture and OC that benefits farmers as well as reducing atmospheric CO2.  相似文献   

13.
Immobilization of N was measured in a fumigated and in an unfumigated soil by adding (15NH4)2SO4 and following the disappearance of inorganic label from the soil solution and its simultaneous conversion to soil organic N. Calculations based on the measurement of organically-bound 15N gave more consistent values for immobilization than did calculations based on the measurement of the disappearance of label from solution. The fumigated soil immobilized 6.6 μg N g?1 N g?1 soil in 10 days at 25°C, the unfumigated control 4.8 μg. The corresponding gross mineralization rates were 34.9 and 5.6 μg N g?1 soil in 10 days.Addition of 58 μg N as (15NH4)2SO4 to the fumigated soil increased the quantity of the ynlabelled NH4-N extracted at the end of 10 days from 33.8 to 37.8 μg Ng?1 soil, i.e. there was a positive Added Nitrogen Interaction (ANI). The added labelled N produced this ANI, not by increasing the rate of mineralization of organic N, but by standing proxy for unlabelled N that otherwise would have been immobilized.A procedure for calculating biomass N from the size of the flush of mineral N caused by fumigation is proposed. Biomass N (BN) is calculated from the relationship BN = F'N/0.68 where F'N is [(N in fumigated soil incubated for 10 days — (N in unfumigated soil incubated for 10 days)].  相似文献   

14.
Pre-plant fumigation of agricultural soils with a combination of methyl bromide (MeBr) and chloropicrin (CP) to control nematodes, soil-borne pathogens and weeds has been a common practice in strawberry (Fragaria X ananassa Duchesne) production since the 1960s. MeBr will be phased out by 2005, but little is known about the impacts of alternative fumigants on soil microbial processes. We investigated the response of microbial biomass and enzyme activities in soils fumigated over two years with MeBr+CP and the alternatives propargyl bromide (PrBr), InLine, Midas and CP. Results were compared to control soils, which were not fumigated for the last 4-5 years for Watsonville and Oxnard, respectively, but had a 10 year history of MeBr+CP fumigation (history soils). Soil samples (0-15 cm) were taken from two sites in the coastal areas of California, USA, in Watsonville and Oxnard, at peak strawberry production after two years of repeated application. In addition to the soil enzymes, the activities of purified reference enzymes of β-glucosidase, acid phosphatase and arylsulfatase were assayed before and after fumigation with MeBr+CP and alternative biocides. At the Oxnard site, microbial respiration significantly decreased in soils fumigated with MeBr+CP (P=0.036), while microbial biomass C and N showed no response to fumigation at both sites. These results may indicate that fumigation promotes the growth of resistant species or that soil microorganisms had recovered at the time of sampling. Repeated soil fumigation with MeBr+CP significantly decreased the activities of β-glucosidase and acid phosphatase at the Watsonville site, and dehydrogenase activity at the Oxnard site. Although, enzyme activities in soils fumigated with PrBr, InLine, Midas and CP were lower compared to the control soil, effects were, in general, not significant. Fumigation with MeBr+CP and alternatives reduced the activities of purified reference enzymes by 13, 76 and 28% for acid phosphatase, β-glucosidase and arylsulfatase, respectively. Mean enzyme protein concentrations in fumigated agricultural soils were 2.93, 0.105, and 2.95 mg protein kg−1 soil for acid phosphatase, β-glucosidase and arylsulfatase, respectively, all lower than in control soils. Organic matter turnover and nutrient cycling, and thus, the long-term productivity of agricultural soils seem unaffected in soils repeatedly fumigated with PrBr, InLine, Midas and CP.  相似文献   

15.
两种测定土壤微生物量氮方法的比较初探   总被引：6，自引：0，他引：6  
用氯仿熏蒸-0.5mol/L的K2SO4直接浸提,280nm紫外比色法和熏蒸-淹水培养法测定了20种有机质、全氮和速效氮差异较大的土样的土壤微生物量N。研究结果表明,两种方法测得20种土样的土壤微生物量N数值呈极显著正相关;280nm紫外比色法操作步骤简单、产生误差的环节少、测定所需时间短、且测定数据比熏蒸-淹水培养法有更好的重现性。初步认为,280nm紫外比色法来反映土壤微生物量的大小。结果还表明,两种方法的测定结果都与土壤的全氮含量呈极显著正相关关系,与有机碳含量有一定的正相关关系,与速效氮无明显的相关关系;但在不同的土壤类型上,与全氮、有机碳和速效氮的相关性有所不同。用280nm紫外比色法测定两种土壤的新鲜和风干样的微生物生物量的结果说明,可用风干土经预培养后测定土壤微生物生物量。风干土样的预培养时间初步确定为10天。  相似文献   

16.
An incubation experiment was carried out to investigate whether salinity at high pH has negative effects on microbial substrate use, i.e. the mineralization of the amendment to CO2 and inorganic N and the incorporation of amendment C into microbial biomass C. In order to exploit natural differences in the 13C/12C ratio, substrate from two C4 plants, i.e. highly decomposed and N-rich sugarcane filter cake and less decomposed N-poor maize leaf straw, were added to two alkaline Pakistani soils differing in salinity, which had previously been cultivated with C3 plants. In soil 1, the additional CO2 evolution was equivalent to 65% of the added amount in the maize straw treatment and to 35% in the filter cake treatment. In the more saline soil 2, the respective figures were 56% and 32%. The maize straw amendment led to an identical immobilization of approximately 48 μg N g−1 soil over the 56-day incubation in both soils compared with the control soils. In the filter cake treatment, the amount of inorganic N immobilized was 8.5 μg N g−1 higher in soil 1 than in soil 2 compared with the control soils. In the control treatment, the content of microbial biomass C3-C in soil 1 was twice that in soil 2 throughout the incubation. This fraction declined by about 30% during the incubation in both soils. The two amendments replaced initially similar absolute amounts of the autochthonous microbial biomass C, i.e. 50% of the original microbial biomass C in soil 1 and almost 90% in soil 2. The highest contents of microbial biomass C4-C were equivalent to 7% (filter cake) and 11% (maize straw) of the added C. In soil 2, the corresponding values were 14% lower. Increasing salinity had no direct negative effects on microbial substrate use in the present two soils. Consequently, the differences in soil microbial biomass contents are most likely caused indirectly by salinity-induced reduction in plant growth rather than directly by negative effects of salinity on soil microorganisms.  相似文献   

17.
Changes in soil moisture determine the reproductive, respiratory, and metabolic activities of soil microorganisms and hence the rate of microbial nitrogen (N) mineralization. Soil moisture also affects the feeding activity and movement of soil invertebrates. Bacterial and fungal grazing by soil invertebrates such as Collembola and nematodes is known to increase N mineralization by increasing the reproductive, respiratory, and metabolic activities of microorganisms. Therefore, to assess the effect of soil moisture on N mineralization, faunal responses need to be considered. We used microcosms to investigate the effect of soil moisture on N mineralization mediated by a species of Collembola, Folsomia candida Willem. We used four moisture levels corresponding to matric potentials of ?42.5, ?11.8, ?0.8, and ?0.5 kPa and investigated the effects of these on Collembola with respect to feeding activity, growth, and contribution to N mineralization. The microbial biomass and ratio of bacterial to fungal biomass tended to increase with increasing soil moisture. Collembola feeding activity and growth increased with increasing soil moisture conditions. Collembola significantly enhanced N mineralization in soil at water potentials of ?11.8 and ?0.5?kPa. The greatest relative increase in N mineralization attributed to Collembola occurred in the ?11.8?kPa treatment. The change in contribution of the Collembola to N mineralization with soil moisture was most likely induced by changes in Collembola feeding activity and microbial community structure. The growth in body length of the Collembola was significantly greater at higher moisture conditions than at the lowest moisture condition, indicating that increases in both metabolic activity and biomass of the Collembola population contributed to the enhanced N mineralization.  相似文献   

18.
Tillage management can affect crop growth by altering the pore size distribution, pore geometry and hydraulic properties of soil. In the present communication, the effect of different tillage management viz., conventional tillage (CT), minimum tillage (MT) and zero-tillage (ZT) and different crop rotations viz. [(soybean–wheat (S–W), soybean–lentil (S–L) and soybean–pea (S–P)] on pore size distribution and soil hydraulic conductivities [saturated hydraulic conductivity (Ksat) and unsaturated hydraulic conductivity {k(h)}] of a sandy clay loam soil was studied after 4 years prior to the experiment. Soil cores were collected after 4 year of the experiment at an interval of 75 mm up to 300 mm soil depth for measuring soil bulk density, soil water retention constant (b), pore size distribution, Ksat and k(h). Nine pressure levels (from 2 to 1500 kPa) were used to calculate pore size distribution and k(h). It was observed that b values at all the studied soil depths were higher under ZT than those observed under CT irrespective of the crop rotations. The values of soil bulk density observed under ZT were higher in 0–75 mm soil depth in all the crop rotations. But, among the crop rotations, soils under S–P and S–L rotations showed relatively lower bulk density values than S–W rotation. Average values of the volume fraction of total porosity with pores <7.5 μm in diameter (effective pores for retaining plant available water) were 0.557, 0.636 and 0.628 m3 m−3 under CT, MT and ZT; and 0.592, 0.610 and 0.626 m3 m−3 under S–W, S–L and S–P, respectively. In contrast, the average values of the volume fraction of total porosity with pores >150 μm in diameter (pores draining freely with gravity) were 0.124, 0.096 and 0.095 m3 m−3 under CT, MT and ZT; and 0.110, 0.104 and 0.101 m3 m−3 under S–W, S–L and S–P, respectively. Saturated hydraulic conductivity values in all the studied soil depths were significantly greater under ZT than those under CT (range from 300 to 344 mm day−1). The observed k(h) values at 0–75 mm soil depth under ZT were significantly higher than those computed under CT at all the suction levels, except at −10, −100 and −400 kPa suction. Among the crop rotations, S–P rotation recorded significantly higher k(h) values than those under S–W and S–L rotations up to −40 kPa suction. The interaction effects of tillage and crop rotations affecting the k(h) values were found significant at all the soil water suctions. Both S–L and S–P rotations resulted in better soil water retention and transmission properties under ZT.  相似文献   

19.
Soil compaction can affect the turnover of C and N (e.g. by changing soil aeration or by changing microbial community structure). In order to study this in greater detail, a laboratory experiment simulating total soil porosities representative of field conditions in cropped and pasture soils was set up. Soils were silty clay loams (Typic Endoaquepts) from a site that had been cropped with cereals continuously for 28 years, a permanent pasture and a site that had been cropped with maize continuously for 10 years. Soils from the three sites were compacted into cores to different total porosities (corresponding bulk densities ranging from 0.88 to 1.30 Mg m−3). The soil cores were equilibrated to different matric potentials (ranging from −1 to −100 kPa), yielding values for the fraction of air-filled pores of < 0.01 to 0.53 m3 m−3, and then incubated at 25°C for 21 days. C-mineralization was on average 15, 33 and 21 μg C g−1 day−1 for soils from the cropped, pasture and maize sites, respectively, and was positively correlated with soil water contents. Net N-mineralization showed a similar pattern only for well-aerated, high total porosity cores (corresponding bulk density 0.88 Mg m−3) from the pasture soil. Denitrification at < 0.20 m3 m−3 for the fraction of air-filled pores may have caused the low N-mineralization rates observed in treatments with high water content or low porosity. Microbial biomass estimates decreased significantly with increasing water contents if measured by fumigation-extraction, but were not significantly affected by water content if estimated by the substrate-induced respiration method. The degree of soil compaction did not affect the microbial biomass estimates significantly but did affect microbial activity indirectly by altering aeration status.  相似文献   

20.
We carried out a study to see the effect of contaminated water of Nullah Dek on fine rice paddy and straw yields and trace elements accumulation in different parts of rice plants and soil. A site was selected near the bank of Nullah Dek at Kot Pindi Das in the District of Sheikhupura, Pakistan. The water of this nullah is contaminated by industrial effluents carrying different micronutrients. This water was employed to grow rice crop. Water samples were collected before transplanting and during the season with 15 days interval for analysis from 20 July to 1 November 2002 from a spot near village Shamke. Three fine rice varieties, viz. Super Basmati, Shaheen Basmati and Basmati 2000 were transplanted. These rice varieties were grown up to maturity. Paddy and straw yields data were recorded. Six composite soil samples from three random spots were collected from the experimental site before the start of the study to see the status of trace elements in soil. After the harvest of rice crop, soil, paddy and straw samples were analysed for Zn, Cu, Fe and Mn. The chemical analysis of Nullah Dek water showed that total salts concentration was greater than the safe limit, i.e. electric conductance (EC) > 1.0 dS m−1. Even sodium adsorption ratio (SAR) was very high, but there was no problem of high residual sodium carbonate (RSC). Zn, Cu, Fe and Mn were present but within safe limits. The water of Nullah Dek remained within permissible limits of irrigation from onset of rainy season till 15 October. There was an increase in EC, SAR and trace elements concentrations after 15 October but within safe limits. Soil analysis revealed its saline nature, devoid of sodicity. Among trace elements, the zinc ranged between deficiency (<0.5 mg kg−1) and adequate limits (>1.0 mg kg−1). Copper, Mn and Fe were present in adequate amounts. After the harvest of rice crop there was a slight decrease in pH, ECe and SAR at both the depths, while the concentrations of all trace elements were slightly increased with more in upper layer than the lower layer. Shaheen Basmati produced the maximum paddy yield followed by Basmati 2000 and then Super Basmati. The chemical analysis of paddy samples indicated a sufficient accumulation of zinc (1.68–1.78 mg kg−1), copper (1.38–1.45 mg kg−1), iron (6.12–6.37 mg kg−1) and manganese (2.22–2.42 mg kg−1). Analysis of rice straw also showed sufficient accumulation of zinc (27.50–28.50 mg kg−1), copper (20.0–20.50 mg kg−1), iron (270–280 mg kg−1) and manganese (2.38–2.41 mg kg−1).  相似文献   

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