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1.
大豆籽粒维生素E含量的QTL分析 总被引：3，自引：0，他引：3
维生素E(VE)具有提高人体免疫力、抗癌、预防心血管疾病等保健作用,从大豆中提取的VE安全性更高。本研究采用高效液相色谱技术(HPLC)检测大豆BIEX群体(Essex×ZDD2315)维生素E的α-生育酚、γ-生育酚和δ-生育酚含量。应用QTLNetwork 2.1软件分别检测到8个和12对控制大豆维生素E及组分含量的加性和互作QTL。α-生育酚含量加性和互作QTL累计贡献值分别为8.68%(2个)和15.57%(4对),γ-生育酚含量加性和互作QTL累计贡献值分别为8.59%(2个)和11.57%(2对),δ-生育酚含量加性和互作QTL累计贡献值分别为5.44%(1个)和17.61%(3对),维生素E总含量的加性和互作QTL累计贡献值分别为11.39%(3个)和9.48%(3对)。未检测到维生素E及组分含量和环境互作的QTL。未定位到的微效QTL累计贡献值为66.16%~75.32%,说明未定位到的微效基因的变异占2/3以上。各性状的遗传构成中,未检测出的微效QTL份额最大,加性QTL和互作QTL贡献相差不大。在育种中应考虑常规方法聚合微效QTL与标记辅助方法聚合主要QTL相结合。 相似文献
2.
3.
Zhenfeng Jiang Binbin Zhang Weili Teng Yingpeng Han Xue Zhao Desheng Sun Zhongchen Zhang Wenbin Li 《Euphytica》2011,177(3):431-442
The oil accumulation in the developing soybean seed has been shown to be a dynamic process with different rates and activities
at different phases affected by both genotype and environment. The objective of the present study was to investigate additive,
epistatic and quantitative trait loci (QTL) × environment interaction (QE) effects of the QTL controlling oil filling rate
in soybean seed. A total of 143 recombinant inbred lines (RILs) derived from the cross of Charleston and Dongnong 594 were
used in this study to obtain 2 years of field data (2004 and 2005). A total of 26 QTL with significantly unconditional and
conditional additive (a) effect and/or additive × environment interaction (ae) effect at different filling stages were identified on 14 linkage groups. Among the QTL with significant a effects, 18 QTL showed positive effects and 6 QTL had negative effects on seed filling rate of oil content during seed development.
A total of 29 epistatic pairwise QTL underlying seed filling rate were identified at different filling stages. About 28 pairs
of the QTL showed additive × additive epistatic (aa) effects and 14 pairs of the QTL showed aa × environment interaction (aae) effects at different filling stages. QTL with aa and aae (additive × additive × environment) effects appeared to vary at different filling stages. Our results demonstrated that oil
filling rate in soybean seed were under genetic, developmental and environmental control. 相似文献
4.
大豆叶茸毛形态对抗虫性、耐旱性等均有重要作用。本研究利用2个重组自交系群体NJRIKY (KY)和NJRIXG (XG)进行叶面茸毛密度和长度的遗传与QTL定位分析。结果表明,2个性状在2个群体中均有大幅度变异,存在不同程度的超亲分离,两者有极显著负相关(r= –0.49和–0.62),叶面茸毛密度的遗传率(75.7%~76.8%)高于叶面茸毛长度的遗传率(45.2%~62.9%);检测到2个叶面茸毛密度主效QTL (XG群体的PD1-1和KY群体的PD12-1,表型贡献率分别达20.7%和21.7%);两群体叶面茸毛密度遗传构成中加性QTL贡献率占20.7%~36.2%,互作QTL只占0%~1.4%,而未定位到的微效QTL所占份额很大,为38.1%~56.1%,是以往只用定位程序而未注意遗传构成解析所没有发现的特点;未在KY中检测到叶面茸毛长度加性QTL,互作QTL贡献率也仅4.2%,而微效QTL贡献率达58.7%;但在XG中叶面茸毛长度加性QTL Pl1-1和Pl12-1贡献率分别达18.3%和22.5%,占主要成分,互作QTL和微效QTL贡献均较小,说明该性状两群体的遗传构成有很大差异。大豆叶面茸毛密度和长度的遗传涉及多个效应不同的基因/QTL。 相似文献
5.
Recombinant inbred lines (RILs) derived from a cross between Brassica rapa L. cv. ‘Sampad’, and an inbred line 3‐0026.027 was used to map the loci controlling silique length and petal colour. The RILs were evaluated under four environments. Variation for silique length in the RILs ranged from normal, such as ‘Sampad’, to short silique, such as 3‐0026.027. Three QTL, SLA3, SLA5 and SLA7, were detected on the linkage groups A3, A5 and A7, respectively. These QTL explained 36.0 to 42.3% total phenotypic variance in the individual environments and collectively 32.5% phenotypic variance. No additive × additive epistatic interaction was detected between the three QTL. Moreover, no QTL × environment interaction was detected in any of the four environments. The number of loci for silique length detected based on QTL mapping agrees well with the results from segregation analysis of the RILs. In case of petal colour, a single locus governing this trait was detected on the linkage group A2. 相似文献
6.
Soybean [Glycine max (L.) Merr.] is the principal oilseed crop in the world. Soybean oil has various industrial and food applications. The quality of soybean oil is determined by its fatty acid composition. Palmitic, stearic, oleic, linoleic and linolenic are the predominant fatty acids in soybean oil. The objective of this study was to determine the associations of simple sequence repeat (SSR) molecular markers with minor differences in fatty acids in soybean oil thereby detecting modifier quantitative trait loci (QTL) which could further improve soybean oil quality. To achieve this objective, 101 F6-derived recombinant inbred lines (RIL) from a population whose parents did not contain major mutant fatty acid alleles were developed from a cross of N87-984-16 × TN93-99. Fatty acids were determined by gas chromatography. Heritability estimates on an entry mean basis for fatty acids ranged from 65.8 to 77.3% for palmitic and linoleic acids, respectively. Molecular marker Satt537 located on molecular linkage group (MLG) D1b was associated with palmitic acid and Satt168 and Satt249 located on MLG B2 and J, respectively were associated with stearic acid. Molecular markers Satt185 or Satt268 (which are within 0.6 cM of each other) located on MLG E were consistently associated with oleic and linoleic acid, and Satt263 and Satt235 located on MLG E and G, respectively were associated with linolenic acid. The lack of markers associated with multiple fatty acids suggests the possibility of independently changing fatty acid levels to achieve a desirable composition, except for regions common to all saturated fatty acids. Phenotypic variation explained by the fatty acids modifier QTL ranged from 10 to 22.5%. These modifier QTL may be useful in making minor improvements to further enhance the quality of soybean oil. 相似文献
7.
Younan Ouyang Fanrong Zeng Jieyun Zhuang Shengmiao Yu Lianfeng Zhu Qianyu Jin Guoping Zhang 《Euphytica》2007,156(3):311-318
High iron levels in rice soils represent a major problem for seedling establishment and crop growth, and rapid coleoptile
elongation is the mechanism for the rice to cope with the induced stress. Quantitative trait loci (QTLs) analysis for coleoptile
elongation rate (CER) in rice (Oryza sativa L.) was performed to study the inheritance of CER and its response to Fe nutrition. A recombinant inbred line (RIL) population
of 244 lines derived from the cross zhenshan97B/miyang46 was germinated in 2004 under four Fe concentrations (0, 1.79, 7.16,
and 14.32 mM). Seven QTLs with additive effects of stimulating CER were detected under the four Fe concentrations and they
were localized on chromosome 1, 4, 5 and 7 with LOD ranging from 2.88 to 15.94 and their contribution to total phenotypic
variance ranging from 4.17% to 15.87%, respectively. In addition, 21 QTLs with additive × additive epistasis were detected
on all chromosomes but 4 and 9. The detected QTLs with additive effect mainly came from the male parent ZS97B. The detected
number of QTLs with additive and epistatic effects for CER varied with Fe concentration. An additive QTL with G × Fe effect
was detected between RZ460 and RZ730 markers of chromosome 1 using multi-environmental model of QTL Mapper 1.6 and considering
Fe concentration as an environmental factor. The pattern of CER in the different Fe concentrations was well characterized
by the genetic model of quantitative traits. It was found that some RILs had higher CER than both parents in each Fe concentration. 相似文献
8.
An additive-dominance, additive × additive (ADAA) and genotype × environment interaction mix model was used to study the genetic
control of β-carotene and l-ascorbic acid in six basic generations (P1, P2, F1, F2, BC1P1 and BC1P2) of tomato derived from the cross CDP8779 accession (Solanum lycopersicum L.) × CDP4777 accession (S. lycopersicum var. cerasiforme). The study was performed in two environments: (1) open field; (2) protected environment, consisting of hydroponic cultivation
in a glasshouse. The results indicate that β-carotene accumulation was mainly additive (32.2% of the genetic component), with
a small dominant component (4.2%) and an important additive × environment interaction contribution (63.6%). In target environments
with moderate to high temperatures and no limiting radiation, this the expression additive × environment interaction could
substantially enhance the β-carotene content. This trait showed also a high narrow-sense heritability (h
2 = 0.62). Ascorbic acid accumulation was also mainly additive (61.7% of the genetic component), with a minor additive epistatic
component (21.5%). This epistatic effect caused a negative heterosis that reduced the positive main additive effect. Nevertheless,
in the described target environments, the additive × environment interaction contribution (16.8%) may enhance the ascorbic
acid content and compensate for the negative heterosis effect. The total narrow-sense heritability of this trait can be considered
useful (h
2 = 0.52). In conclusion, the CDP4777 accession is a very interesting donor parent for the joint improvement of β-carotene
(without diminishing lycopene content) and ascorbic acid content in commercial nutraceutical tomato breeding programmes; the
F1 hybrids derived from this accession showed nearly 450% of the commonly reported average β-carotene content and close to 130%
of the ascorbic acid content of the female parent. 相似文献
9.
Tocopherols are natural antioxidants in vegetable oils and are important dietary nutrients. Enhanced tocopherol content has become an important objective in oilseed rape breeding. A segregating DH population was tested for 2 years at two locations in replicated field trials. Genotypic differences occurred for α‐, γ‐ and total tocopherol content as well as α/γ‐tocopherol ratio, but highly significant genotype x environment interactions resulted in low heritabilities. Using a mixed‐model composite interval mapping approach between one and five QTL with additive and/or additive x environment interaction effects could be mapped for α‐, γ‐ and total tocopherol content and α/β‐tocopherol ratio. In addition, one to six locus pairs with epistatic interaction effects were identified, indicating a strong contribution of epistasis to trait variation. In total, the additive and epistatic effects explained between 28% (α‐tocopherol content) and 73% (total tocopherol content) of the genotypic variance in the population, with individual QTL and locus pairs contributing between 7.5 and 29.2% of variance. Considering the low heritabilities of the tocopherol traits, the results of this study indicate that marker‐assisted selection may be an efficient strategy in a breeding program for enhanced tocopherol content in rapeseed. 相似文献
10.
陆地棉中G6主要性状主效和上位性QTL分析 总被引：2，自引：0，他引：2
利用基于混合线性模犁的复合区间作图法对重组近交系(recombinant inbreed line,RIL)"中G6"进行QTL定位,生育期性状共定位了主效QTL位点5个,上位性QTL位点4对,纤维品质性状定位了主效QTL位点1个,上位性QTL位点6对,产量性状定位了主效QTL位点3个,上位性QTL位点5对.其中定位的果枝始节、吐絮期、上半部平均长度、衣分的主效QTL位点均距离最近标记1 cM以下,这有利于在育种实践中主效QTL跟踪检测.定位的霜前花率主效QTL位点具有较高的加性效应和遗传贡献率,应进行QTL精细定位、图位克隆,将会对早熟性育种工作有一定的推动意义.定位的马克隆值、衣分和予指总的遗传贡献率均在30%以上,对性状特征均影响显著.对主效及上位性QTL位点进行遗传效应分析,验证了前人有关数量性状遗传符合主基因与多基因混合遗传的论断,认为此模型是研究数量性状遗传的有效途经;对主效及上位性QTL位点进行A、D亚基因组定位,并对主效及上位性QTL位点在A、D亚基因组上的分布及互作方式进行了详细的分析.全文认为上位性QTL位点和主效QTL位点一样在物种遗传变异和聚合育种中起着重要作用. 相似文献
11.
以丰产性好、抗旱力强的栽培大豆晋豆23为母本,山西农家品种半野生大豆灰布支黑豆为父本杂交衍生的447个RIL作为供试群体。将亲本及447个家系分别于2011、2012和2013年采用随机试验种植,按照标准测量叶长、叶宽和叶柄长3个性状,并于2012年8月1日和8月8日和2013年8月2日和8月9日各测量1次叶绿素含量。采用QTLNETwork 2.0混合线性模型分析方法和主基因+多基因混合遗传分离分析法,对大豆叶片性状和叶绿素含量进行遗传分析和QTL间的上位性和环境互作效应研究。结果表明,叶长受2对加性-加性×加性上位性混合主基因控制,叶宽受3对等效主基因控制,叶柄长受4对加性-加性×加性上位性主基因控制,叶绿素含量受4对加性主基因控制;检测到10个与叶长、叶宽、叶柄长和叶绿素含量相关的QTL,分别位于A1、A2、C2、H_1、L和O染色体。其中2个叶长QTL分别位于C2和L染色体,是2对加性×加性上位互作效应及环境互作效应QTL;3个叶宽加性与环境互作QTL分别位于A2、C2和O染色体;2个叶柄长QTL分别位于L和O染色体;3个叶绿素含量QTL分别位于A1、C2和H_1染色体。叶片性状和叶绿素含量的遗传机制较复杂,加性效应、加性×加性上位互作效应及环境互作效应是大豆叶片性状和叶绿素含量的重要遗传基础。建议大豆分子标记辅助育种中,一方面要考虑起主要作用的QTL,另一方面要注重上位性QTL的影响,这对于性状的遗传和稳定表达具有积极的意义。 相似文献
12.
Quantitative trait loci (QTLs) influencing textural properties (hardness, adhesiveness, springiness, cohesiveness, gumminess,
chewiness, and resilience)of wheat for Chinese northern-style steamed bread were studied using a doubled haploid (DH) population
containing 168 lines derived from a cross between elite Chinese wheat cultivars Huapei 3 and Yumai 57 (Triticum aestivum L.). The DH population and parents were grown in 2007 and 2008 in Tai’an and 2008 in Suzhou. QTL analyses were performed using
the software QTL Network version 2.0 and IciMapping v2.2 based on the mixed linear model. Thirty nine putative QTLs were detected
on 14 chromosomes: viz. 1A, 2A, 3A, 4A, 6A, 1B, 2B, 3B, 5B, 6B, 7B, 5D, 6D, and 7D, and single QTLs explained 3.91–35.17%
of the phenotypic variation. Eight pairs of QTLs with epistatic effects and/or epistasis × environment (AAE) effects were
detected for adhesiveness, resilience, hardness, and cohesiveness on chromosomes 2A, 1B and 3D. Several co-located QTLs with
additive effects were detected on chromosomes 2B, 5D, 6A, 3A, 3B and 6D. Two clusters of three QTLs for steamed bread textural
properties (chewiness, gumminess, and hardness) and for adhesiveness, cohesiveness and resilience were detected on chromosome
2B. Two co-located QTLs with epistatic effects were detected on chromosomes 1B and 3A. Both additive effects and epistatic
effects were important for Chinese steamed bread textural properties, which were also subject to environmental modifications.
The information obtained in this study will be useful for manipulating QTLs determining Chinese steamed bread textural properties
by molecular marker-assisted selection. 相似文献
13.
大豆脂肪酸组分的胚、细胞质和母体遗传效应分析 总被引：1，自引：0，他引：1
利用5个大豆品种配制20个杂交组合,采用广义种子遗传模型分析了大豆脂肪酸组分的胚、细胞质和母体植株等3套遗传体系的基因主效应和基因型×环境效应。棕榈酸含量、硬脂酸含量和亚油酸含量是以基因型×环境互作效应为主。亚麻酸和油酸的遗传主效应和基因型×环境互作效应相近。在脂肪酸组分的遗传主效应中,棕榈酸、硬脂酸和亚油酸含量是以胚主效应为主。油酸含量和亚麻酸含量以细胞质主效应为主。在基因型×环境互作方差中,脂肪酸组分以极显著的胚互作方差为主。亚麻酸含量是以基因的加性效应和加性×环境互作效应为主,棕榈酸含量、硬脂酸含量、油酸含量和亚油酸含量以基因的显性和显性×环境互作效应为主。棕榈酸含量和油酸含量是以普通狭义遗传率为主。硬脂酸、亚油酸含量和亚麻酸含量以互作狭义遗传率为主。在普通狭义遗传率中,棕榈酸含量、油酸含量和亚麻酸含量以细胞质普通遗传率和母体普通遗传率为主。在互作狭义遗传率中,油酸含量和亚麻酸含量以胚互作狭义遗传率为主,亚油酸含量以母体植株互作遗传率为主。棕榈酸含量、硬脂酸含量、油酸含量和亚油酸含量以细胞质及母体选择响应和互作选择响应为主,亚麻酸含量的胚普通选择响应和互作选择响应为主。 相似文献
14.
Mapping quantitative trait loci (QTLs) underlying seed vitamin E content in soybean with main,epistatic and QTL × environment effects 
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下载免费PDF全文 Soybean (Glycine max (L.) Merr.) seed contains small amounts of tocopherol, a non‐enzymatic antioxidant known as lipid‐soluble vitamin E (VE). Dietary VE contributes to a decreased risk of chronic diseases in humans and has several beneficial effects on resistance to stress in plants, and increasing VE content is an important breeding goal for increasing the nutritional value of soybean. In this study, quantitative trait loci (QTLs) underlying VE content with main, epistatic and QTL × environment effects were identified in a population of F5 : 6 recombinant inbred lines from a cross between ‘Hefeng 25’ (a low‐VE cultivar) and ‘OAC Bayfield’ (a high‐VE cultivar). A total of 18 QTLs were detected that showed additive main effects (a) and/or additive × environment interaction effects (ae) in different environments. Moreover, 19 epistatic pairs of QTLs were found to be associated with α‐tocopherol (α‐Toc), γ‐tocopherol (γ‐Toc), δ‐tocopherol (δ‐Toc) and total VE (TE) contents. The QTLs identified in multienvironments could provide more information about QTL by environment interactions and could be useful for the marker‐assistant selection of soybean cultivars with high seed VE contents. 相似文献
15.
Kun-Pu Zhang Guang-Feng Chen Liang Zhao Bin Liu Xian-Bin Xu Ji-Chun Tian 《Euphytica》2009,165(3):471-484
Flour color is an important trait in the assessment of flour quality for the production of many end products. In this study,
quantitative trait loci (QTLs) with additive effects, epistatic effects, and QTL × environment (QE) interactions for flour
color in bread wheat (Triticum aestivum L.) were studied, using a set of 168 doubled haploid (DH) lines derived from a Huapei 3 × Yumai 57 cross. A genetic map was
constructed using 283 simple sequence repeats (SSR) and 22 expressed sequence tags (EST)-SSR markers. The DH and parents were
evaluated for flour color in three environments. QTL analyses were performed using QTLNetwork 2.0 software based on a mixed
linear model approach. A total of 18 additive QTLs and 24 pairs of epistatic QTLs were detected for flour color, which were
distributed on 19 of the 21 chromosomes. One major QTL, qa1B, closely linked to barc372 0.1 cM, could account for 25.64% of the phenotypic variation of a* without any influence from the environments. So qa1B could be used in the molecular marker-assisted selection (MAS) in wheat breeding programs. The results showed that both additive
and epistatic effects were important genetic basis for flour color, and were also sometimes subject to environmental modifications.
The information obtained in this study should be useful for manipulating the QTLs for flour color by MAS in wheat breeding
programs.
Kun-Pu Zhang and Guang-Feng Chen contributed equally to this study. 相似文献
16.
Quantitative trait loci underlying soybean seed tocopherol content with main additive,epistatic and QTL × environment effects 下载免费PDF全文
下载免费PDF全文 Huancheng Liu Guanglu Cao Depeng Wu Zhenfeng Jiang Yingpeng Han Wenbin Li 《Plant Breeding》2017,136(6):924-938
Soybean (Glycine max [L.] Merrill) seeds are a major source of tocopherols (Toc), which could significantly improve immune system health of human and prevent or treat many serious diseases. Selection for higher Toc contents of seeds could increase nutritional value of soybean‐derived food, laying on an important breeding goal for many soybean breeders. The present objectives of the work were to evaluate various genetic effects of QTL associated with individual and total Toc content based on a RIL population (“Beifeng 9” × “Freeborn”) in six environments to improve the efficiency of molecular marker‐assisted selection (MAS) for high‐Toc breeding. The results described that eighteen, thirteen, eleven and thirteen QTL were associated with α‐Toc, γ‐Toc, δ‐Toc and total Toc content, respectively, and have additive main effects (a) and/or additive × environment interaction effects (ae) in certain environments. Among them, four QTL for α‐Toc, two QTL for γ‐Toc, one QTL for δ‐Toc and four QTL for total Toc could increase α‐Toc, γ‐Toc, δ‐Toc and total Toc content via significant a effect, respectively, which have stronger stability in different years and locations. It implied a value for MAS. Additionally, twenty‐five, fifteen, eleven and twenty epistatic pairwise QTL associated with α‐Toc, γ‐Toc, δ‐Toc and total Toc contents, respectively, were detected. The genetic information of the QTL effects obtained here would be beneficial for breeding soybean variety with high‐Toc content by MAS. 相似文献
17.
Amita Mohan Pawan Kulwal Ravinder Singh Vinay Kumar Reyazul Rouf Mir Jitendra Kumar Manoj Prasad H. S. Balyan P. K. Gupta 《Euphytica》2009,168(3):319-329
A framework linkage map comprising 214 molecular marker (SSR, AFLP, SAMPL) loci was prepared using an intervarietal recombinant
inbred line (RIL) mapping population of bread wheat. The RIL population that was developed from the cross SPR8198 (red-grained
and PHS tolerant genotype) × HD2329 (white-grained and PHS susceptible genotype) following single seed descent segregated
for pre-harvest sprouting (PHS). The RIL population and parental genotypes were evaluated in six different environments and
the data on PHS were collected. Using the linkage map and PHS data, genome-wide single-locus and two-locus QTL analyses were
conducted for PHS tolerance (PHST). Single-locus analysis following composite interval mapping (CIM) detected a total of seven
QTL, located on specific arms of five different chromosome (1AS, 2AL, 2DL, 3AL and 3BL). These seven QTL included two major
QTL one each on 2AL and 3AL. Two of these seven QTL were also detected following two-locus analysis, which resolved a total
of four main-effect QTL (M-QTL), and 12 epistatic QTL (E-QTL), the latter involved in 7 QTL × QTL interactions. Interestingly,
none of these M-QTL and E-QTL detected by two-locus analysis was involved in Q × E and Q × Q × E interactions, supporting
the results of ANOVA, where genotype × environment interaction were non-significant. The QTL for PHS detected in the present
study may be efficiently utilized for marker-aided selection for enhancing PHST in bread wheat. 相似文献
18.
大豆籽粒硬实加性和上位性QTL定位 总被引：2，自引：0，他引：2
硬实是植物种子的普遍特性, 是影响大豆种子发芽率、生存能力及储存期的重要数量性状, 同时影响着大豆的加工品质。本实验通过对大豆籽粒硬实性状的加性和上位性互作QTL (quantitative trait locus)分析, 明确控制大豆籽粒硬实的重要位点及效应, 旨在为进一步解析硬实性状复杂的遗传机制提供理论依据。以冀豆12和地方品种黑豆(ZDD03651)杂交构建的包含186个家系的F6:8和F6:9重组自交系群体为材料, 采用WinQTL Cartographer V. 2.5的复合区间作图法(composite interval mapping, CIM)定位不同年份的籽粒硬实性状相关的加性QTL, 同时采用IciMapping 4.1软件中的完备区间作图法(inclusive composite interval mapping, ICIM)检测籽粒硬实性状的加性及上位性QTL。共检测到3个籽粒硬实性状相关的加性QTL, 分别位于第2、第6和第14染色体, 遗传贡献率范围为5.54%~12.94%。同时检测到4对上位性互作QTL, 分别位于第2、第6、第9、第12和第14染色体, 可解释的表型变异率为2.53%~3.47%。同时检测到籽粒硬实性状加性及上位性互作QTL, 且上位性互作多发生在主效QTL间或主效QTL与非主效QTL间, 表明上位性互作效应在大豆籽粒硬实性状的遗传基础中具有重要的作用。 相似文献
19.
Yashoda Jadhav Surendra S. Manohar Gururaj Sunkad Viswanatha P. Kannalli Manish K. Pandey Murali T. Variath Shasidhar Yaduru Praveen Kona Rajeev K. Varshney Janila Pasupuleti 《Plant Breeding》2019,138(6):748-760
Parents and 318 F8 recombinant inbred lines (RILs) derived from the cross, TAG 24 × ICGV 86031 were evaluated for peanut bud necrosis disease (PBND) resistance and agronomic traits under natural infestation of thrips at a disease hotspot location for 2 years. Significant genotype, environment and genotype × environment interaction effects suggested role of environment in development and spread of the disease. Quantitative trait loci (QTL) analysis using QTL Cartographer identified a total of 14 QTL for six traits of which five QTL were for disease incidence. One quantitative trait locus q60DI located on LG_AhII was identified using both QTL Cartographer and QTL Network. Another QTL q90DI was detected with a high PVE of 12.57 using QTL Cartographer. A total of nine significant additive × additive (AA) interactions were detected for PBND disease incidence and yield traits with two and seven interactions displaying effects in favour of the parental and recombinant genotype combinations, respectively. This is the first attempt on QTL discovery associated with PBND resistance in peanut. Superior RILs identified in the study can be recycled or released as variety following further evaluations. 相似文献
20.
Hao Tang Hongbo Wang Maojie He Mingyu Zhang Yangshan Hu Zhi Li Tianheng Ren Zhenglong Ren Benju Yan 《Euphytica》2017,213(10):235
Falling number (FN) is an inner quality trait in wheat (Triticum aestivum L.) ultimately determining the end use of wheat kernels. In this 3-year study, 171 recombinant inbred lines derived from Chuannong17 (a 1BL/1RS tranlocation parent) × Mianyang11 were planted in the Sichuan Basin, a rainfed agricultural area in southwestern China. In this climate, we found that FN had significant differences between 1BL/1RS translocation lines and non-1BL/1RS translocation lines in two of the 3 years and the heavy fluctuation of rainfall and temperature resulted in decreasing FN in grain filling period. We used 191 simple sequence repeats markers to construct a genetic linkage map and then detected 11 additive effect FN quantitative trait loci (QTL) on chromosomes 2B, 3D, 4A, 4D, 6B and 7D, explaining 5.48–31.91% of the phenotypic variance. The FN QTL on chromosomes 4A, 4D and 6B were major or stable and detected at least in 2 years, whereas the Qfn.sicau-3D.1 in 2015 year explained the maximum phenotypic variation (about 31.91%). Furthermore, FN QTLs additive and epistatic effects as well as their interactions with environment were estimated by a mixed linear model approach. We found that the additive effect QTLs had no significant additive × environment interaction, while the paired QTLs had significant additive × additive epistatic effects however none had a significant additive × additive epistasis × environment interaction effect, excluding the paired QTLs Qfn.sicau-3B/Qfn.sicau-5B. 相似文献