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新城疫是危害养禽业的一种主要传染病,通过其抗体水平监测,分析免疫状态,对鸡群科学饲养,确保养鸡业健康发展。本次检测沂南县某蛋鸡场血样50份,分离血清,通过微量血凝及血凝抑制试验(HI)进行新城疫抗体水平监测。试验结果表明样品平均HI抗体效价为9.4log2,说明整场免疫合格,不需要补种,新城疫疫苗能达到最佳效果,能抵抗新城疫病毒的侵入。 相似文献
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本次检测费县某蛋鸡场血样30份,分离血清,通过微量血凝及血凝抑制试验(HI)进行新城疫、禽流感H5亚型(Re-5株)抗体监测。结果表明,该鸡场新城疫HI抗体效价≥5log2的占87%,新城疫免疫合格;而禽流感抗体效价≥5log2的占67%,禽流感H5亚型免疫失败,鸡场必须补免或重免禽流感H5亚型二联油乳剂灭活苗。 相似文献
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为找到新城疫抗体监测的最佳方法,选择郑州市六县九区不同规模鸡场,实施不同新城疫抗体监测方法的比对试验与研究,对血凝试验(HA)和血凝抑制试验(HI)、酶联免疫吸附试验(EUSA)以及新城疫抗体免疫金标试纸条监测新城疫抗体进行了分析和研究,结果表明,HI方法不仅节约成本,省时省力,而且监测结果准确,适合大批量的新城疫抗体... 相似文献
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海原县部分鸡场新城疫免疫现状调查 总被引：2，自引：1，他引：1
应用血凝试验和血凝抑制试验对采自海原县部分鸡场的239份血样进行了新城疫免疫抗体的检测。血清检测结果表明,所有检样的平均免疫保护率为76.99%。说明海原县部分鸡场中新城疫免疫抗体水平偏低,应及时进行免疫接种并加强抗体监测。 相似文献
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《养殖与饲料．饲料世界》2020,(4)
为了解山东省日照市养禽场(户)新城疫疫苗的免疫效果,应用血凝抑制试验方法对全市9家养禽场(户)的315份血清样品进行实验室检测。检测结果显示,新城疫抗体检测中,检测315份,免疫合格290份,抗体水平合格率达到92.1%,符合国家要求。 相似文献
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新城疫、禽流感作为危害养禽业的主要传染病,通过其抗体水平监测,分析免疫状态,对鸡群合理饲养,确保养鸡业健康发展。本次检测沂南县某蛋鸡场血样50份,分离血清,通过微量血凝及血凝抑制试验(HI)进行新城疫、禽流感H5亚型(Re-5株)抗体水平监测。试验结果表明,新城疫平均HI抗体效价为9.4 log 2,禽流感H5亚型(Re-5株)平均HI效价为7.64 log 2,说明整场免疫合格,但鸡群需要补种禽流感H5亚型二联油乳剂灭活苗。 相似文献
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《当代畜牧》2017,(2)
为正确评估高致病性禽流感疫苗的免疫效果和质量。运用小孩7号头皮针在鸡的翅静脉上采血,在395个散养户的土鸡、45个规模场的蛋鸡、10个规模场的肉鸡,运用HI(血凝抑制试验)对高致病性禽流感(H5型)抗体实验室监测,随后进行抗体水平科学评估。监测了395个散养户的土鸡,205个散养户的鸡免疫不合格,监测的血清样本免疫合格率56.96%。监测了55个规模养蛋鸡场和肉鸡场,3个规模场抗体不合格,52个规模场鸡场抗体合格,总共监测规模场的血清样本免疫合格率81.56%。雏鸡的最佳首免时间应为7~10日龄时,2周后,用禽流感-新城疫重组二联活疫苗(r L-H5)加强免疫1次。经过科学地按程序2次免疫接种的鸡,保护力很高,免疫后抗体水平有效保护时间可达4~6个月。 相似文献
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为了调查通海县规模化蛋鸡养殖场禽流感和新城疫的流行情况,本试验结合养殖场的免疫情况,随机采集10个蛋鸡养殖场卵黄样品148份,采用血凝(HA)和血凝抑制(HI)实验进行抗体检测分析。结果显示:10个蛋鸡场中HY1和HHX蛋鸡场ND抗体平均值不合格,MGQ蛋鸡场H9抗体平均值不合格,H5-6抗体平均值全都合格,XJW、LHY、WY、QJY、YGL蛋鸡场H5-7抗体平均值不合格;新城疫抗体合格率为89.87%,禽流感H9、H5-6和H5-7亚型抗体合格率分别为91.98%、77.63%、42.19%,其新城疫抗体水平良好,禽流感H5-7亚型抗体整体水平偏低,其中,有少数样品H5-7亚型抗体效价偏高,怀疑以前有野毒感染过;新城疫和禽流感H9和H5-6抗体极差都≤5,其抗体水平较为均匀,而禽流感H5-7抗体极差相差大,QJY和YGL蛋鸡场的H5-7抗体极差为10。这些数据为通海县蛋鸡养殖场禽流感和新城疫的防控及免疫工作提供参考。 相似文献
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《饲料工业》2019,(18):54-58
应用电感耦合等离子-质谱技术(ICP-MS),建立饲料中钠、镁、铬、锰、铁、铜、锌、砷、硒、镉和铅等元素的测定方法。对饲料样品的前处理方法、仪器工作参数和11种元素标准曲线进行优化;并以加标回收、分析方法比对和重复测试说明方法的准确性和精密性。方法在0～1 000 ng/ml范围内线性良好,仪器检出限为0.557 7～5.072 ng/ml,具有良好的精密度,其回收率在88.1%～104.4%之间,相对标准偏差小于5.0%。同时与原子吸收和原子荧光方法进行比对,测定结果相近。所建立的方法简单、快速,可替代原子吸收和原子荧光方法测定饲料中的11种金属元素,为饲料的质量控制提供理想的元素分析方法。 相似文献
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In experiment 1, 6 pregnant mares received a concentrate that contained a trace mineral premix that provided 14.3 mg Cu, 40 mg Zn, 28 mg Fe, 28 mg Mn, 0.08 mg Co, 0.16 mg I, and 0.16 mg Se/kg concentrate (group A). Seven mares received the same concentrate plus 502 mg Zn and 127 mg Cu once daily (group B). No differences (P > .05) in foal growth data, or Cu, Zn, and Fe concentrations of mare milk, mare serum, or foal serum were observed. In experiment 2, 6 pregnant mares received the same concentrate as group A (group C), and 8 mares received the same concentrate fortified with 4× the trace mineral premix (group D). Group C mares had higher serum Zn concentration at 1 day (P < 0.01) and 56 days (P < 0.04). Group C mares had higher milk Fe concentration at 28 days (P < .01), and group D mares had higher milk Cu concentration at 56 days (P < .01). Group C foals had higher serum Cu concentration at 14 days (P < .03). The results from this study provide no evidence to indicate that supplementing late gestating and lactating mares with higher dietary trace mineral levels than those recommended currently by NRC has any influence on foal growth and development, or on the Cu, Zn, and Fe concentrations of the mare milk, mare serum, or foal serum. 相似文献
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Breed differences for weight (CW), height (CH), and condition score (CS) were estimated from records (n = 12,188) of 2- to 6-yr-old cows (n = 744) from Cycle IV of the U.S. Meat Animal Research Center's Germplasm Evaluation (GPE) Program. Cows were produced from mating Angus and Hereford dams to Angus, Hereford, Charolais, Shorthorn, Galloway, Longhorn, Nellore, Piedmontese, and Salers sires. Samples of Angus and Hereford sires were 1) reference sires born from 1962 through 1970 and 2) 1980s sires born in 1980 through 1987. The mixed model included cow age, season of measurement and their interactions, year of birth, pregnancy-lactation code (PL), and breedgroup as fixed effects for CW and CS. Analyses of weight adjusted for condition score included CS as a linear covariate. The model for CH excluded PL. Random effects were additive genetic and permanent environmental effects associated with the cow. Differences among breed groups were significant (P < 0.05) for all traits and were maintained through maturity with few interchanges in ranking. The order of F1 cows for weight was as follows: Charolais (506 to 635 kg for different ages), Shorthorn and Salers, reciprocal Hereford-Angus (HA) with 1980s sires, Nellore, HA with reference sires, Galloway, Piedmontese, and Longhorn (412 to 525 kg for different ages). Order for height was as follows: Nellore (136 to 140 cm), Charolais, Shorthorn, Salers, HA with 1980s sires, Piedmontese, Longhorn, Galloway and HA with reference sires (126 to 128 cm). Hereford and Angus cows with reference sires were generally lighter than those with 1980s sires. In general, breed differences for height followed those for weight except that F1 Nellore cows were tallest, which may in part be due to Bos taurus-Bos indicus heterosis for size. 相似文献
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采用液相色谱-串联质谱法测定动物尿液中11种β-受体激动剂残留量,对标准溶液、体积、质谱峰面积、浓缩过程及回收率等测定不确定度因素进行了分析,通过评定各不确定度分量及标准不确定度,得出11种β-受体激动剂的扩展不确定度在0.7 ～ 1.1 ng/mL范围内.由各因素对合成不确定度的贡献比分析可知,影响较大的因素为试验回收率及标准溶液浓度. 相似文献
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K Reifenberg M Stahl U L?sch 《Zentralblatt für Veterin?rmedizin. Reihe B. Journal of veterinary medicine. Series B》1989,36(3):191-198
A simple two step procedure for the isolation of caprine, ovine, bovine, equine, canine, porcine and human peripheral blood granulocytes is described. After enrichment of granulocytes by centrifugation, contaminating erythrocytes are lysed hypotonically. Recovery, purity, and viability of the granulocyte suspensions are determined. FACScan analysis of the cell suspensions measuring cellular size by forward and sideward light scatter is compared with the corresponding analysis of whole blood leukocytes. Constituencies of the isolated cell suspensions and loss of granulocyte subpopulations through isolation procedure is discussed with regard to granulocyte function assays. 相似文献