共查询到20条相似文献，搜索用时 15 毫秒
1.
Molecular analysis of protein assembly in muscle development 总被引：13，自引：0，他引：13
The challenge presented by myofibril assembly in striated muscle is to understand the molecular mechanisms by which its protein components are arranged at each level of organization. Recent advances in the genetics and cell biology of muscle development have shown that in vivo assembly of the myofilaments requires a complex array of structural and associated proteins and that organization of whole sarcomeres occurs initially at the cell membrane. These studies have been complemented by in vitro analyses of the renaturation, polymerization, and three-dimensional structure of the purified proteins. 相似文献
2.
Perturbation of red cell membrane structure during intracellular maturation of Plasmodium falciparum 总被引：3，自引：0，他引：3
An experimental approach, which in this study was applied to the malarial system, can be used to analyze the molecular structure and organization of individual phospholipids in a wide variety of biological membranes. Electron spin resonance spectroscopy was used to investigate the structural modifications of the major red cell phospholipids that occur in erythrocyte membranes infected with the human malarial parasite, Plasmodium falciparum. These modifications were correlated with the intracellular developmental stage of the parasite. Phosphatidylcholine, phosphatidylethanolamine, and phosphatidylserine were increasingly disordered (fluidized) as infection progressed. This disordering occurred at different rates and to varying extents. 相似文献
3.
To establish infection in the host, malaria parasites export remodeling and virulence proteins into the erythrocyte. These proteins can traverse a series of membranes, including the parasite membrane, the parasitophorous vacuole membrane, and the erythrocyte membrane. We show that a conserved pentameric sequence plays a central role in protein export into the host cell and predict the exported proteome in Plasmodium falciparum. We identified 400 putative erythrocyte-targeted proteins corresponding to approximately 8% of all predicted genes, with 225 virulence proteins and a further 160 proteins likely to be involved in remodeling of the host erythrocyte. The conservation of this signal across Plasmodium species has implications for the development of new antimalarials. 相似文献
4.
1990: annus mirabilis of potassium channels 总被引：17，自引：0，他引：17
C Miller 《Science (New York, N.Y.)》1991,252(5010):1092-1096
Voltage-gated potassium channels make up a large molecular family of integral membrane proteins that are fundamentally involved in the generation of bioelectric signals such as nerve impulses. These proteins span the cell membrane, forming potassium-selective pores that are rapidly switched open or closed by changes in membrane voltage. After the cloning of the first potassium channel over 3 years ago, recombinant DNA manipulation of potassium channel genes is now leading to a molecular understanding of potassium channel behavior. During the past year, functional domains responsible for channel gating and potassium selectivity have been identified, and detailed structural pictures underlying these functions are beginning to emerge. 相似文献
5.
[目的]探讨通过纳升液相色谱-飞行时间质谱检测人永生化表皮细胞的膜蛋白质的方法。[方法]对人永生化表皮细胞进行贴壁培养,然后对处于对数生长期细胞的膜蛋白进行提取、富集和酶解,用nano—HPLC—TOF／TOF—MS对蛋白质进行鉴定,最后用生物信息学技术对鉴定蛋白进行分析和归类。[结果]在鉴定的15个蛋白中,与细胞膜相关的蛋白有9个,占比例的60％。生物信息学分析表明,这些蛋白在分子功能上主要涉及催化,结构分子活性和结合;在生物学进程上,这些蛋白主要归类为代谢,细胞成分形成和细胞生物进程等。[结论]纳升色谱-飞行时间质谱是鉴定膜蛋白的有效方法。 相似文献
6.
1 Introduction Hypophosphorus in cows is very common in the world.Sixty percent samples of pas-turage have the low content of phosphorus(<0 .1 5% ) in Ireland and40 % soil in some district of the Soviet Union in normal dissection.The phenomenon of hypopohosphatemia,hemolysis and hemoglobinuria resulted from low- phosphorous of circumstance(<0 .1 0 % inpasturage,rough 0 .50︼g/ g soil for utilize in pasture field) and microthermal climate (Jan-uary to March) in the west part of Heilongji… 相似文献
7.
【目的】从鸡卵泡膜细胞蛋白中筛选和鉴定与膜联蛋白A2（ANXA2）相互作用的细胞蛋白并进行功能分析,为深入研究ANXA2调控鸡卵泡发育的作用机制提供理论依据。【方法】制备开产后30周龄贵州黄鸡的卵泡膜细胞,提取卵泡膜总蛋白后利用His Pull-Down联合质谱技术（LC-MS/MS）从卵泡膜细胞中筛选出与鸡ANXA2互作的细胞蛋白,然后通过GO数据库和KEEG数据库分别进行GO功能富集分析及KEEG信号通路注释分析,并利用STRING Version 11.0绘制蛋白互作网络图。【结果】通过His Pull-Down联合LC-MS/MS共鉴定获得41个鸡ANXA2互作细胞蛋白,GO功能富集分析发现这些互作细胞蛋白在分子功能、生物学进程和细胞组成均发挥作用。其中,在分子功能方面主要涉及蛋白结合（占58.06%）、催化活性（占19.35%）、核糖体结构（占16.13%）及细胞骨架结构组成（占6.45%）,在生物学进程方面主要参与细胞骨架（占19.35%）、刺激反应（占19.35%）、翻译（占16.13%）、代谢过程（占12.90%）、细胞迁移（占12.90%）、蛋白折叠（占9.68%）和蛋白运输（占9.68%）,而细胞组分显示以定位于细胞膜的蛋白为主（占32.26%）。鸡ANXA2蛋白互作细胞蛋白参与的KEEG信号通路主要有应激反应、代谢、翻译、信号转导、免疫系统和蛋白定位等。鸡ANXA2互作细胞蛋白互作网络可分为3条,即CNN2-FN1-MYH9-MYH10-ACTN1-CSRP1、ANXA1-ANXA2-ENO1-PRDX4-GPI-ATP5B-PRDX3-HSPA8-TUBB2A和CCT7-CCT4-GNB2L1-ATP5A1-RPS3-RPS3A-RPL23A-RPL22-RPS7;互作细胞蛋白间存在复杂的互作关系,其中又以膜联蛋白A1（ANXA1）与烯醇化酶-1（ENO1）及ANXA2的互作关系最明显。【结论】鸡ANXA2互作细胞蛋白主要参与细胞骨架形成、应对刺激和翻译等生物学过程,涉及应激反应、代谢、翻译、信号转导、免疫及蛋白定位等信号通路。其中,PRDX3、PRDX4、MYH9和TCSC可能通过与ANXA2蛋白相互作用而参与鸡卵巢相关疾病的发生,而ANXA1与ANXA2相互作用可能在鸡卵泡的发育及排卵过程中发挥重要调节作用。 相似文献
8.
Hiller NL Bhattacharjee S van Ooij C Liolios K Harrison T Lopez-Estraño C Haldar K 《Science (New York, N.Y.)》2004,306(5703):1934-1937
Malaria parasites secrete proteins across the vacuolar membrane into the erythrocyte, inducing modifications linked to disease and parasite survival. We identified an 11-amino acid signal required for the secretion of proteins from the Plasmodium falciparum vacuole to the human erythrocyte. Bioinformatics predicted a secretome of >320 proteins and conservation of the signal across parasite species. Functional studies indicated the predictive value of the signal and its role in targeting virulence proteins to the erythrocyte and implicated its recognition by a receptor/transporter. Erythrocyte modification by the parasite may involve plasmodial heat shock proteins and be vastly more complex than hitherto realized. 相似文献
9.
10.
[目的]运用生物信息学方法预测动物双歧杆菌AD011锚定于细胞壁的蛋白并进行功能分析,为后续研究该菌与宿主相互作用的分子机制提供基础资料。[方法]用Phobius和SignalP 4.0软件对动物双歧杆菌AD011全基因组编码蛋白中细胞壁蛋白进行预测,同时采用COG(Cluster of Orthologous Groups of proteins)功能数据库对预测的细胞壁蛋白进行功能注释和聚类分析。[结果]动物双歧杆菌AD011基因组1 518个编码蛋白中,11个蛋白含有细胞壁的锚定基序,6个蛋白含有LP×TG基序,1个蛋白含有Lys M基序,1个蛋白含有PG结合区域,2个蛋白含有SLH结构域,1个蛋白含有NLPC_P60结构域。细胞壁蛋白功能分析结果显示,11个细胞壁蛋白中,9个蛋白没有功能注释,2个蛋白(NLP/P60 protein和1,4-beta-N-acetylmuramidase)参与细胞壁和细胞膜的生物合成。[结论]动物双歧杆菌AD011大多数细胞壁蛋白功能未知,还需在以后的研究中进行进一步的功能注释。 相似文献
11.
Identification and location of brain protein 4.1 总被引：8，自引：0，他引：8
Protein 4.1 is a membrane skeletal protein that converts the low-affinity interaction between spectrin and actin into a high-affinity ternary complex of spectrin, protein 4.1, and actin that is essential to the structural stability of the erythrocyte. Pig brain was shown to contain an 87-kilodalton immunoreactive analog of protein 4.1 that has partial sequence homology with pig erythrocyte protein 4.1 and the same location as spectrin in the cortical cytoplasm of neuronal and glial cell types of the cerebellum. 相似文献
12.
Surface area of human erythrocyte lipids: reinvestigation of experiments on plasma membrane 总被引：9，自引：0，他引：9
Ratios of the lipid monolayer area to the erythrocyte surface area are 2:1 at low surface pressures and approach 1: 1 at collapse pressures. Un saturated phospholipids in cholesterol-phospholipid complexes of membrane ex tracts resemble their saturated derivatives at collapse pressures. Area ratio and phospholipid area data are related by an equation that tests hypothetical values for molecular areas used in membrane models. 相似文献
13.
Chemostimulatory protein: a new type of taste stimulus 总被引：3，自引：0，他引：3
R H Cagan 《Science (New York, N.Y.)》1973,181(94):32-35
Three taste-active proteins have recently been discovered. It is proposed that two of these (monellin and thaumatin) should be classified as chemostimulatory proteins because of their sensory effect; these two proteins taste intensely sweet. The third protein (miraculin), a taste-modifier protein, changes the normal sour taste of acids to sweet. The taste-modifier protein, miraculin, occurs in the fruit of the tropical plant Synsepalum dulcificum. Though itself not sweet, it is able to change the taste of acids from sour to sweet after the tongue has been treated with the protein. Miraculin is a basic glycoprotein with a molecular weight of 44,000. Monellin, a chemostimulatory protein, is found in the fruit of a different tropical plant, Dioscoreophyllum cumminsii. It has been characterized as a basic protein with a molecular weight of 10,700 that contains no carbohydrate. Thaumatin, another chemostimulatory protein, occurs in the fruit of a third tropical plant, Thaumatococcus daniellii. Like monellin, it is a basic protein that contains no carbohydrate. Its molecular weight is around 21,000. Certain gross similarities among the three proteins have been noted. Their basic ionic character and some features of the amino acid compositions are similar. Little is known of the structural features of the chemostimulatory proteins that are required for eliciting their intense sweetness; they are of the order of 10(5) times more effective than sucrose. The precise role of the tertiary structure in their biological activity is not known but appears to be an important area for further study. The relatively large size (11,000 to 21,000 molecular weight) of the chemostimulatory proteins provides indirect evidence that the initial interaction of these stimuli with taste receptor cells occurs at the plasma membrane. 相似文献
14.
[目的]分析两歧双歧杆菌PRL2010菌体外表面蛋白的功能。[方法]以两歧双歧杆菌PRL2010基因组序列为研究对象,采用Perry等人分析革兰氏阳性菌菌体外表面蛋白(PSE蛋白)的Inmembrane方法,同时采用COG功能数据库对预测的外表面蛋白进行功能注释和聚类分析。[结果]PRL2010外表面蛋白包括28个细胞壁蛋白、12个脂蛋白、182个细胞膜蛋白、38个分泌蛋白。PRL2010外表面蛋白的功能分析结果显示,大多数外表面蛋白与细胞壁、细胞膜的生物合成,无机离子转运与代谢,防御机制,碳水化合物转运与代谢,氨基酸转运与代谢等有关。[结论]该研究可为探讨该菌与宿主相互作用的分子机制奠定基础。 相似文献
15.
布鲁氏菌的外膜蛋白在维持细胞结构方面发挥着重要作用,其中,有些外膜蛋白与细菌本身的毒性相关,成为了重要的抗原决定簇相关分子。Omp31分子就是猪型布鲁氏菌的一个重要的抗原表位分子,在研制亚单位疫苗方面有重要作用。本论文以猪型布鲁氏菌Omp31基因作为研究对象,利用DNAStar生物学软件对Omp31分子的立体结构、分子的表面可能性、亲水性、蛋白骨架区的柔韧性、分子的抗原指数进行了详细分析。结果显示：猪型布鲁氏菌Omp31分子一级结构的氨基酸序列中存在4段可能的抗原表位区段,其中,51~80氨基酸残基组成的区段和193~228氨基酸残基组成的区段的抗原指数较高,成为抗原决定簇的可能性很大。 相似文献
16.
[目的]为研究饥饿胁迫下鱼类的生理生化变化提供参考。[方法]采用传统瑞氏染色法研究饥饿胁迫下大鳞副泥鳅外周红细胞的形态变化。[结果]大鳞副泥鳅的成熟红细胞逐渐发生变化,先出现核膜溶解,然后出现细胞形变,接着出现核质扩散和细胞膜溶解,最后整个成熟红细胞膜完全溶解,核染色反应由紫黑色变为紫红色最后变为粉红色。大鳞副泥鳅的幼稚红细胞则明显增多。[结论]在饥饿胁迫下大鳞副泥鳅外周红细胞出现核异常,主要表现为核膜溶解和核质扩散,因此红细胞核异常可以作为饥饿胁迫的指标。 相似文献
17.
[目的]运用生物信息学方法预测两歧双歧杆菌PRL2010锚定于细胞壁的蛋白和功能分析,为后续研究该菌与宿主相互作用的分子机制奠定基础。[方法]用Phobius和Signal P 4.0软件对两歧双歧杆菌PRL2010全基因组编码蛋白中细胞壁蛋白进行预测,同时采用COG(Cluster of Orthologous Groups of proteins)功能数据库对预测的细胞壁蛋白进行功能注释和聚类分析。[结果]两歧双歧杆菌PRL2010基因组1 706个编码蛋白中,28个蛋白含有细胞壁的锚定结构域。细胞壁蛋白功能分析结果显示,28个细胞壁蛋白中,21个蛋白没有功能注释,7个蛋白有功能注释,其中1个蛋白(exo-alpha-sialidase)参与碳水化合物代谢与转运,2个蛋白(bacteriophage endolysin和cell wall-associated protein)参与细胞壁和细胞膜的生物合成,2个蛋白(hypothetical protein BBPR_0440和Subtilisin family peptidase)参与蛋白质翻译后修饰,1个蛋白(beta-n-acetylhexosaminidase Nag Z)参与细胞内运输、分泌和囊泡运输,1个蛋白(metallophosphoesterase)功能只是预测的。[结论]两歧双歧杆菌PRL2010的大多数细胞壁蛋白功能未知,还需要在以后的研究中进一步分析和注释。 相似文献
18.
Fraser HB Hirsh AE Steinmetz LM Scharfe C Feldman MW 《Science (New York, N.Y.)》2002,296(5568):750-752
High-throughput screens have begun to reveal the protein interaction network that underpins most cellular functions in the yeast Saccharomyces cerevisiae. How the organization of this network affects the evolution of the proteins that compose it is a fundamental question in molecular evolution. We show that the connectivity of well-conserved proteins in the network is negatively correlated with their rate of evolution. Proteins with more interactors evolve more slowly not because they are more important to the organism, but because a greater proportion of the protein is directly involved in its function. At sites important for interaction between proteins, evolutionary changes may occur largely by coevolution, in which substitutions in one protein result in selection pressure for reciprocal changes in interacting partners. We confirm one predicted outcome of this process-namely, that interacting proteins evolve at similar rates. 相似文献
19.
Membrane structure: some general principles 总被引：55，自引：0，他引：55
M S Bretscher 《Science (New York, N.Y.)》1973,181(100):622-629
The arrangement of lipids and some proteins in the erythrocyte membrane has been discussed. The conclusions from this are listed here as a set of general guidelines for the structure of membranes of higher organisms: some of these rules may be wrong. But at this stage it seems useful to sharpen our thoughts in this way and thereby focus attention on various specific points. 1) The basis of a membrane is a lipid bilayer with (i) choline phospholipids and glycolipids in the external half and (ii) amino (and possibly some choline) phospholipids in the cytoplasmic half. There is effectively no lipid exchange across the bilayer (unless enzymatically catalyzed) (68). 2) Some proteins extend across the bilayer. Where this is so, they will in general have carbohydrate on their surface remote from the cytoplasm. This carbohydrate may prevent the protein diffusing out of the membrane into the cytoplasm; it acts as a lock on the protein. 3) Just as lipids do not flip-flop, proteins do not rotate across the membrane. Lateral motion or rotation of lipids and proteins in the plane of the bilayer may be expected. 4) Most membrane protein is associated with the inner, cytoplasmic, urface of the membrane. Proteins are not usually associated exclusively with the outer half of the lipid bilayer. 5) Membrane proteins are a special class of cytoplasmic proteins, not of secreted proteins. 相似文献
20.
[目的]分析动物双歧杆菌AD011暴露外表面蛋白的种类和功能,有助于了解该菌株的各项生理功能以及为探讨该菌与宿主相互作用的分子机制奠定基础。[方法]以动物双歧杆菌AD011基因组序列为研究对象,采用Inmembrane方法和COG功能数据库对预测的外表面蛋白进行功能注释和聚类分析。[结果]AD011菌体暴露外表面蛋白数目为193个,包括11个细胞壁蛋白、20个脂蛋白、暴露外表面的膜蛋白140个、22个细胞外蛋白。AD011外表面蛋白的功能分析结果显示,193个外表面蛋白中,含有较大比例的蛋白没有功能注释(78个),115个蛋白具有COG功能注释。在有功能注释的蛋白中,所占比例较大的是氨基酸转运与代谢(16个),无机离子转运与代谢(14个),细胞壁、细胞膜生物合成(13个),碳水化合物转运与代谢(10个),防御机制(10个)等有关蛋白。[结论]AD011菌体外表面蛋白与营养物质的吸收和转运,细胞壁、细胞膜生物合成,防御机制有关。 相似文献