共查询到17条相似文献，搜索用时 187 毫秒
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黑木耳多糖对糖尿病小鼠降血糖作用 总被引：7，自引：0，他引：7
目的：研究黑木耳多糖对正常小鼠及四氧嘧啶小鼠的降血糖作用。方法：黑木耳多糖灌胃30d，观察其对正常小鼠血糖的影响、对四氧嘧啶糖尿病小鼠血糖的影响。结果：黑木耳多糖对正常小鼠血糖有降低作用；糖尿病小鼠血糖显著降低。结论：黑木耳多糖具有显著降血糖作用。 相似文献
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灵芝多糖降血糖作用的研究 总被引：6，自引：0，他引：6
目的：研究灵芝多糖对四氧嘧啶致高血糖小鼠、去甲肾上腺素致高血糖小鼠及正常小鼠血糖水平的影响。方法：制备四氧嘧啶致糖尿病小鼠模型及去甲肾上腺素致高血糖小鼠模型,给药2周后取血测定血糖水平。结果：灵芝多糖能明显降低四氧嘧啶所致糖尿病小鼠及去甲肾上腺索所致高血糖小鼠的血糖水平,其中高剂量组与模型对照组相比均有显著意义（P〈0．05）,而对正常小鼠血糖水平影响较小,低、中、高剂量组与正常对照组相比均无显著意义（P〉0．05）。结论：灵芝多糖对四氧嘧啶致高血糖小鼠及去甲肾上腺素致高血糖小鼠具有明显降血糖作用,而对正常小鼠血糖水平影响较小。 相似文献
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蜜环菌菌索多糖对小鼠血糖及急性毒性作用研究 总被引：12，自引：3，他引：12
本文研究了蜜环菌菌索多糖AMP - 1和AMP - 2组分对小鼠血糖及其急性毒性作用。结果表明 :AMP - 1能使正常小鼠的糖耐量增强 ;AMP - 1、AMP - 2均能抑制四氧啶糖尿病小鼠血糖升高 ;AMP - 2能显著降低四氧啶糖尿病小鼠的血糖 ;AMP - 1、AMP - 2 (灌胃剂量为 10g/kg/d)对供试小鼠无毒性作用 ,内脏器官均正常无损 相似文献
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针对刺芹侧耳谷氨酰胺对运动机体的作用开展研究。测定了刺芹侧耳中氨基酸的含量,并对谷氨酸和谷氨酰胺进行了分离。小鼠动物试验表明,谷氨酰胺能够促进小鼠肌肉增长,对机体的耐氧能力和血糖浓度稳定有一定效果,能够有效提高机体运动能力和耐久力。 相似文献
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粗毛黄褐孔菌多糖降血糖试验研究 总被引：2，自引：0，他引：2
通过研究粗毛黄褐孔菌多糖降血糖的作用,分别用不同剂量野生粗毛黄褐孔菌多糖,灌胃正常小鼠与四氧嘧啶致糖尿病小鼠,结果显示,野生粗毛黄褐孔菌多糖对正常小鼠无明显影响,对糖尿病小鼠在给药21 d后,中剂量组和高剂量组与阴性对照组间差异极显著,与阳性对照组之间差异不显著;用野生粗毛黄褐孔菌与人工栽培、液体发酵菌丝体多糖相同剂量灌胃糖尿病小鼠21 d后,三者降血糖作用无显著差异,均能一定程度降低糖尿病小鼠血糖。 相似文献
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桦褐孔菌人工培养菌丝体、菌核与野生菌核多糖的降血糖比较试验研究 总被引：6，自引：1，他引：6
目的是探讨桦褐孔苗人工培养菌丝体、菌核与野生菌核多糖的降血糖作用。方法：采用四氧嘧啶（200mg／kg体重）腹腔注射复制小鼠高血糖模型。分别给予小鼠应用桦褐孔菌人工培养菌丝体、菌核与野生菌核多糖提取物按不同剂量灌胃，葡萄糖氧化酶法测定各组的血糖水平。结果：桦褐孔菌菌丝体多糖提取物不同剂量组对四氧嘧啶型高血糖模型小鼠的血糖均有抑制作用，对正常小鼠无明显降血糖作用。桦褐孔菌人工培养菌丝体、菌核与野生菌核多糖三者的降血糖作用无显著性差异。结论：桦褐孔菌人工培养菌丝体、菌核与野生菌核多糖提取物对四氧嘧啶型高血糖模型小鼠均有降血糖作用。对正常小鼠无明显降血糖作用。 相似文献
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AIM：To establish the animal model of insulinoma and to analyze the properties of insulinoma for further study. METHODS：The hormone-releasing ability of rat insulinoma cell line INS-1 was detected in vitro. INS-1 cells were transplanted into the left kidney capsule of nude mice. The islets of the animals were destroyed by intraperitoneal injection of streptozocin (STZ) 3 d before transplantation or 2 weeks after transplantation. The venous blood was sampled, and the level of blood glucose less than 2.8 mmol/L was defined as successful establishment of insulinoma model. Different irritants were given to the model animals, and the changes of blood glucose and insulin content in serum were observed. The pancreatic tissues and the renal tissues in the injecting sites were taken from all mice for detecting insulin and glucagon by immunohistochemical staining. RESULTS：Insulinoma cells expressed insulin and glucagon at the same time. The blood glucose was less than 2.8 mmol/L 3 to 4 weeks after inoculation of INS-1 cells. Apparent tumor formed in the left kidneys where INS-1 cells were transplanted and the tumor diameters were more than 1 cm. The level of blood glucose transiently increased to higher than the normal level in the mice with tumor cell transplantation after intraperitoneal injection of STZ, and then decreased gradually and returned to less than 2.8 mmol/L after 2 weeks. The level of blood glucose in the normal nude mice after administration of STZ increased significantly. After transplantation of INS-1 cells, the level of blood glucose decreased gradually, and returned to less than 2.8 mmol/L after 4 weeks. After stimulated with high glucose, the blood glucose levels in the mice with 3 methods to establish the insulinoma models showed lower glucose peaks than that in the normal control mice. After stimulated with high glucose plus arginine or acetylcholine in the normal animals, the blood glucose peak was lower than that in the normal animals only stimulated with high glucose, and rapidly recovered to the normal level. However, the levels of blood glucose in the mice with 3 methods to establish the insulinoma models under the same stimulations were significantly higher than that in the mice only stimulated with high glucose. After stimulated with high glucose plus norepinephrine, the blood glucose peak time in the normal animals delayed, and the blood glucose level declined slowly. After stimulated with high glucose plus norepinephrine, the levels of blood glucose in the mice with 3 methods to establish the insulinoma models increased as compared with that in the mice only stimulated with high glucose. Compared with normal control group, serum insulin in insulinoma mice increased significantly. CONCLUSION：The insulinoma animal model is successfully established by transplantation of INS-1 cells into the renal capsule of nude mice. The insulinoma cells express both insulin and glucagon, and are not easily damaged by STZ. 相似文献
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AIM: To investigate the expression of survivin in pancreas in the streptozotocin-induced diabetic mice. METHODS: Low dose of streptozotocin was used to induce diabetes mellitus in BALB/c mice. Body weight and blood glucose concentrations were examined at 1, 2, 3 and 4 weeks after the streptozotocin injection. Expression of survivin mRNA was detected by real-time FQ-PCR. RESULTS: Survivin was expressed in the pancreas of normal BALB/c mice. Low dose of streptozotocin provoked hyperglycaemia with increased survivin expression in the pancreas, but blood glucose concentration and expression of survivin was not significantly changed in control group. CONCLUSION: Survivin is expressed in the pancreas of normal BALB/c mice. Streptozotocin increases survivin expression in the pancreas, which may be related with islets regeneration. 相似文献
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AIM: To study the effects of water decoction of Platycladus orientalis on reducing high blood glucose and lipids in diabetic and hyperlipidemia mice, respectively. METHODS: The mouse model of hyperlipidemia was established. The increasing rate of weight, hepatic index, lipid content of liver and atherosclerosis index (AI) were measured. Blood levels of total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), adiponectin (ADP) and interleukin-6 (IL-6) were detected. According to these indexes, the function of water decoction of Platycladus orientalis for reducing high blood lipids caused by hyperlipidemia was evaluated. At the same time, the model of diabetes mellitus was established. The levels of fasting plasma glucose, fasting plasma insulin and blood tumor necrosis factor-α (TNF-α) were measured. The glucose tolerance test was performed. The insulin resistance index was calculated. The function of water decoction of Platycladus orientalis to reduce high blood glucose caused by diabetes was observed by these above indexes. RESULTS: Water decoction of Platycladus orientalis lowered the increasing rate of weight, hepatic index and lipid content of liver, reduced the blood content of TC, TG and IL-6, and elevated the blood HDL-C and ADP content caused by high-fat diet apparently (P<0.05). The effect of water decoction of Platycladus orientalis at high dose was more effective than that at low dose. Water decoction of Platycladus orientalis obviously alleviated the abnormity of glucose tole-rance test, reduced blood TNF-α and insulin levels, and decreased insulin resistance index caused by diabetes apparently (P<0.05). The effect of the drug at high dose was stronger than that at low dose. CONCLUSION: The water decoction of Platycladus orientalis dose-dependently reduces blood glucose caused by diabetes and blood lipids caused by hyperlipidemia in the mice. The mechanism to reduce blood lipids may be related with elevating blood ADP content and reducing blood IL-6 content, and the mechanism to reduce blood glucose may be related with lowering blood TNF-α content. 相似文献
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LI Yan HE Juan LI Fang-ping LIU Shan-ying LUO Zhao-fan LI Feng YAN Li LIU Dan XU Ming-tong 《园艺学报》2009,25(5):944-948
AIM: To establish the animal model of hyperresistinemia and to observe the effects of resistin on glucose metabolism and insulin sensitivity in vivo. METHODS: We established a mouse model of hyperresistinemia in C57BL/6 mice by intravenous administration of the recombinant adenovirus encoding mouse resistin. Then we observed the fasting blood glucose and insulin levels. We also investigated glucose tolerance by IPGTT, and insulin sensitivity by IPITT. RESULTS: On 5 d after the injection, the concentration of plasma resistin was more than 15-fold higher in Adv-resistin-EGFP-treated mice than that in saline- or Adv-EGFP-treated mice. In the fasting state, no difference in glucose levels was observed among three groups. However, mice injected with Adv-resistin showed higher insulin levels, impaired glucose tolerance and insulin sensitivity. CONCLUSION: Hyperresistinemia affects glucose metabolism in mice and it may play an important role in the pathogenesis of insulin resistance and type 2 diabetes. 相似文献
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AIM: To explore the protective effects of exosome secreted by human umbilical mesenchymal stem cells on cardiac fibrosis in diabetic mouse model. METHODS: Male C57BL/6 mice at 6~8 weeks of age were divided into 3 groups randomly:control group, diabetes mellitus (DM) group and DM+exosome group. To develop mouse DM mo-del, the mice were fed with high-fat diet for 5 weeks, followed by intraperitoneal injection of 45 mg/kg streptozocin once a week for 5 weeks. It was considered as a successful DM model that the blood glucose of the mice was ≥ 16.7 mmol/L. The mice in DM+exosome group were injected with exosome via tail vein. The mice in other 2 groups were injected with saline at the same volume. The heart function was evaluated by color Doppler echocardiography for small animals. The blood samples were collected from abdominal aortas. The blood glucose and non-esterified fatty acids were measured by biochemical colorimetric assay. HE staining was performed to observe the structural changes of myocardial fibers, and Masson staining was used to observe the cardiac fibrosis. RESULTS: The results of echocardiography showed that left ventricular end-diastolic dimension (LVIDd) and left ventricular end-systolic dimension (LVIDs) of diabetic mice were larger than those in control mice (P<0.05 and P<0.01, respectively). The ejection fraction (EF) and fractional shortening (FS) decreased in the diabetic mice (P<0.01). Exosome treatment significant decreased the LVIDs (P<0.01), but increased the EF and FS (P<0.01). The blood glucose and non-esterified fatty acids were significantly increased in the diabetic mice. The injection of the stem cell exosome significantly decreased the blood glucose and non-esterified fatty acids (P<0.01). HE staining observation showed that cardiomyocyte hypertrophy and fragmentation of cardiomyocyte in DM group were more se-rious than those in control group. Masson staining showed that the area of fibrosis in DM group was larger than that in control group (P<0.01), but that in DM+exosome group was reduced (P<0.01). CONCLUSION: Exosome secreted by human umbilical mesenchymal stem cells protects the DM model mice from cardiac fibrosis. 相似文献
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为探讨白菜冻干粉(Chinese Cabbage Freeze-Dried Powder,CCFDP)对高糖高脂饲料饲养小鼠餐后血糖的影响,对血糖筛选合格小鼠分别进行6组处理:正常对照组、高糖高脂饲料饲养造模对照组、造模+同时口服灌胃CCFDP低剂量组、造模+同时口服灌胃CCFDP高剂量组、造模+造模15 d后再同时口服灌胃CCFDP低剂量组、造模+造模15 d后再同时口服灌胃CCFDP高剂量组,45 d后检测小鼠餐后血糖值、血清中总胆固醇(T-CHO)、甘油三酯(TG)、高密脂蛋白(HDL-C)、低密脂蛋白(LDL-C)等指标。结果表明:与模型对照组比较,150 mg/(kg·d)CCFDP能有效降低高糖高脂饲料饲养小鼠的餐后血糖值以及血清TG和LDL-C。CCFDP能显著降低高糖高脂饲料饲养小鼠的餐后血糖,为糖尿病早期防治提供参考。 相似文献
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TIAN He-lin WEI Li-shun XU Zhong-xin KANG Yan-hui ZHAO Ru-tong JIN Dong-ling 《园艺学报》2012,28(2):358-361
AIM: To investigate the relationship between microvessel density (MVD) and expression of vascular endothelial growth factor (VEGF) in glomeruli of diabetic mice. METHODS: Streptozotocin-induced diabetic mice as well as the control mice were involved in this study for 6 weeks. The body weight and blood glucose level of the mice in each group were weekly measured at certain time point. The morphological changes of the kidney were observed under light microscope, and the diameter, perimeter and area of the glomeruli were detected by an image analysis system. The expression of CD34 and VEGF in glomeruli was examined by immunohistochemistry method, and MVD and VEGF index were also calculated. RESULTS: In comparison with the control mice, the blood glucose level was significantly increased,and the body weight was decreased in diabetic mice(P<0.01). The diameter, perimeter and area of glomeruli in diabetic mice were significant greater than those in control mice (P<0.05). Increased expression of CD34 and VEGF in the glomeruli of diabetic mice was observed. Glomerular MVD of diabetic mice was significantly higher than that of the controls (P<0.01), and was positively correlated with the VEGF index (r=0.9979, P<0.05). CONCLUSION: VEGF may promote the angiogenesis in glomeruli of diabetic mice. The increase in VEGF expression may play a role in the pathogenesis of diabetic nephropathy. 相似文献