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4份桃种质挥发性成分的GC-MS分析 总被引：1，自引：0，他引：1
为了分析桃果实中共有的挥发性成分及初步探讨果皮毛和果肉颜色性状对挥发性成分的影响情况,以此2对性状均存在差异的4份桃种质为试材,采用顶空固相微萃取方法提取其成熟果肉中的挥发性成分,并用气相-质谱联用仪对其进行了测定。结果表明:4份桃种质中共鉴定出116种挥发性成分,其主要为酯、醛、醇和烷烃等类物质。从挥发性成分的种类数量上看,白肉桃‘大久保’和‘华光’中检出的挥发性成分种类较多,分别有68和52种;而2份黄肉桃种质中检出的挥发性成分种类均较少。从挥发性成分的种类上看,2份普通桃与2份油桃中的差异挥发性成分总共仅有3种,其分别为反式-2,4-庚二烯醛、戊酸-2,2,4-三甲基-3-异丙酸-异丁酯和脱氧精胍菌素,远低于2份白肉桃与2份黄肉桃种质中差异挥发性成分的数量(13种)。此外,从挥发性成分的类群上看,2份白肉桃含有较多的"青香型"的C6类化合物,而2份黄肉桃含有较多的"果香型"的酯和内酯类化合物。文中综合分析认为,果皮毛的有无和果肉的颜色性状均可能影响果实中挥发性成分的数量和种类,但后者的影响较为明显。 相似文献
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Hirofumi Hirai Aiko Onitsuka Ryuichiro Kondo Kokki Sakai Tomoaki Nishida 《Journal of Wood Science》2001,47(5):374-377
Iron-binding compounds were isolated from a culture ofPhanerochaete sordida YK-624 and were found to bind to Fe(III) preferentially compared with Fe(II). Two iron-binding compounds were purified to near-homogeneity with gel permeation chromatography. Hydrolysis of the iron-binding compounds with 6N hydrochloric acid gave ninhydrin-negative products. The molecular weight of these compounds was 3–5 kDa. These compounds may play an important role in the reduction of extracellular manganese dioxide to Mn(II) by intracellular ferrireductases for lignin degradation by manganese peroxidase. 相似文献
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应用顶空固相微萃取和气相色谱—质谱联用方法,对贵州、云南两省12种山茶属植物物种及类型花的挥发性物质及其相对含量进行分析.共分离鉴定出237种挥发性化合物,占总挥发性化合物种类的99.58％.主要包括醛类、酮类、醇类、酯类、萜烯类、烷烃类、酸类及其他类等8类化合物.以怒江山茶花的挥发物种类最多(88种),离蕊金花茶最少(41种).有51种化合物为12个物种及类型中的多数植物所共有.其中,100％共有的5种;90％共有的8种;80％共有的10种;70％共有的10种;50％共有的18种;50％以下共有的100种.有86种化合物为各物种所独有.归类分析表明,醇类比例最高,达29.87％;萜烯类次之,为27.79％;再次为酯类,为22.48％.化合物中相对含量第1为L-芳樟醇,相对含量达75.94％;第2为(Z)-3-己烯酯,为42.48％;第3为庚烷-2-酮,为31.67％;第4为(Z)-3-己烯-1-醇和(S)-2-庚醇,分别达23.79％和20.95％. 相似文献
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Two new compounds (S)-(+)-butyl-4-methylene-5-oxo-pyrrolidin-2-carboxylate (1) and (5-formylfuran-2-yl)methyl 4-hydroxy-2-methylenebutanoate (2), together with four known compounds (3-6) were isolated from the roots of Polygala tricornis Gagnep. Their structures were determined on the basis of 1D- and 2D-NMR experiments and by comparison with physical data of known compounds. All the isolated compounds were examined for their inhibitory effect on the nitric oxide (NO) production induced by lipopolysaccharide (LPS) in BV-2 microglial cells, and compounds 1 and 2 exhibited pronounced inhibition on the NO production with IC(50) values of 14.1 μM and 1.77 μM, respectively. 相似文献
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Two new phenylethanoid glycosides magnoloside D (1) and E (2), together with nine known compounds, were isolated from the polar part of methanol extract of the stem bark of Magnolia officinalis. The structures of the new compounds were established on the basis of spectral analysis. Anti-spasmodic activity of four major constituents (3, 4, 9 and 11) was tested in isolated colon of rat, compounds 3, 4, and 9 showed inhibition against acetylcholine, with the effect similar to that of magnolol and honokiol. At the same time, antioxidant activity of the isolated compounds was investigated using a DPPH and an ORAC assay. All of the compounds, except compound 8 showed potent antioxidant capacity in the ORAC assay, while compounds 1-5 and 11 exhibited potent antioxidant activity in the DPPH assay. 相似文献
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Salvianolic acid B (Sal B) is the most abundant phenolic compound in Salvia miltiorrhiza, which has been widely used for the treatment of cardiovascular diseases in Oriental medicine. To elucidate structure of the converted compounds of Sal B by decoction in aqueous solution, Sal B (200mg) was decocted in an aqueous solution (200mL) at pH 4.9 and the decocted solution was purified by Sephadex LH-20 column chromatography and preparative HPLC. The 13 converted compounds were isolated and the chemical structures were determined by NMR and MS. In addition to the 4 compounds previously reported as conversion products of Sal B by decoction, 9 compounds were first reported with the complete structure of compounds isolated from decocted Sal B solution and three of the compounds were determined to be novel compounds. In addition, a conversion mechanism of compounds converted by decoction was proposed on the basis of kinetics studies, which reasonably supported the conversion mechanism of Sal B. The 13 compounds seemed to be produced by the hydrolysis of an ester bond, decarboxylation, retro oxa-Michael reaction, hydration, and radical reaction during decoction in aqueous solution. 相似文献
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《Fitoterapia》2014
Two new hydrolysable tannins, chebumeinin A (1) and chebumeinin B (2), together with eight known related compounds (3-10), were isolated from the fruits of Terminalia chebula. The new compounds were structurally determined by analysis of their spectroscopic data and the known compounds characterized by comparing their spectroscopic data with literature values. All isolates were evaluated by an HCV protease inhibition assay, and some compounds were found to be potently active. 相似文献
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Six-year-old cloned Betula pubescens Ehrh. trees, grown outdoors at 65 degrees 01' N, were cut on six dates during the growing season to study coppice shoot development in relation to root-produced cytokinin-like compounds. Bleeding sap was collected over timed intervals for two days after cutting, and endogenous cytokinin-like compounds were measured by ELISA assay in HPLC-purified fractions of xylem sap. Initiation and development of coppice shoots on the clonally propagated plants were comparable to those in seedlings. Coppice shoot initiation was affected by the time of cutting, diminishing significantly after June. Of the cytokinin-like compounds detected in the xylem sap, zeatin riboside-like (ZR) compounds were present in the highest concentrations, and the concentrations of dihydrozeatin riboside-like (DHZR) and isopentenyladenoside-like (IPA) compounds were approximately one third and one eighth of the ZR concentrations, respectively. The concentration of cytokinin-like compounds was positively correlated with xylem sap flow rate. The export of cytokinin-like compounds, especially DHZR- and ZR-types, was positively correlated with the initiation and elongation rate of coppice shoots, the number of lateral branches, and the radial growth of the more slowly growing coppice shoots. The export of cytokinin-like compounds collected immediately after cutting may represent the basal value for each tree. This value is probably affected by the size and activity of the root system and may be a relevant estimate for predicting the success of coppicing. 相似文献
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A new guaianolide and a new eudesmanolide were isolated from Lactuca tatarica, as well as eight known sesquiterpenoids. The new compounds were elucidated on the basis of spectroscopic methods including IR, HRESIMS, 1D and 2D NMR, and the known compounds were established by comparing their physical data with those of the corresponding compounds in the literature. 相似文献
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All factors affecting the PCR system in Pinus massoniana Lamb were investigated one by one.The results show that the optimum PCR system of EST-SSR were:2μL 10×Buffer(Mg2+ Free), 30 ng template DNA,0.187 5 mmol/L dNTPs, 3.75 mmol/L Mg2+,8 pmol primer pair,1.0 U Taq DNA poly-merase in total 20μL reaction system. Finally,a total of 11 isolates of P.massoniana was used for testing the stability of the PCR amplification.The results prove that the optimum PCR system was stable,reliable,highly repetitive. 相似文献
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Peng F Tao Q Wu X Dou H Spencer S Mang C Xu L Sun L Zhao Y Li H Zeng S Liu G Hao X 《Fitoterapia》2012,83(3):568-585
Twenty-nine phenolic compounds were isolated from the root bark of fresh (Yunnan) ginger and their structures fully characterized. Selected compounds were divided into structural categories and twelve compounds subjected to in-vitro assays including DPPH radical scavenging, xanthine-oxidase inhibition, monoamine oxidase inhibition, rat-brain homogenate lipid peroxidation, and rat pheochromocytoma PC12 cell and primary liver cell viability to determine their antioxidant and cytoprotective properties. Isolated compounds were also tested against nine human tumor cell lines to characterize anticancer potency. Several diarylheptanoids and epoxidic diarylheptanoids were effective DPPH radical scavengers and moderately effective at inhibiting xanthine oxidase. An enone–dione analog of 6-shogaol (compound 2) was isolated and identified to be most effective at protecting PC12 cells from H2O2-induced damage. Almost all tested compounds inhibited lipid peroxidation. Three compounds, 6-shogaol, 10-gingerol and an enone-diarylheptanoid analog of curcumin (compound 6) were identified to be cytotoxic in cell lines tested, with KB and HL60 cells most susceptible to 6-shogaol and the curcumin analog with IC50 < 10 μM. QSAR analysis revealed cytotoxicity was related to compound lipophilicity and chemical reactivity. In conclusion, we observed distinct compounds in fresh ginger to have biological activities relevant in diseases associated with reactive oxygen species. 相似文献
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Analysis of compounds in dichloromethane extractives for Sawara Falsecypress （Chamaecyparis pisifera） outer heartwood 总被引：1，自引：0，他引：1
The chemical components of dichloromethane extractives for Sawara Falsecypress heartwood were analyzed with GC/MS except for basic chemical composition analysis for heartwood with Chinese standard method. 14 kinds of compounds were idenfifjed according to the computer compounds library data. The major compounds in dichloromethane extractives comprised of terpene and naphthalene derivafives. The experiments of antifungal effects of the dichloromethane extractive on Aspergillus niger were also carded out. The result showed that the dichloromethane extractive from Sawara Falsecypress has no or weak antifungal capability. 相似文献
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Two new compounds, a neolignan (1), and a benzofuran derivative (2), along with 6 known compounds (3-8), were isolated from the aerial parts of Leontopodium leontopodioides. The structures of the new compounds were elucidated as (7R,8S)-3,5'-dimethoxy-4',7-epoxy-8,3'-neolignane-5,9,9'-triol (1) and (2R)-12-hydoxy-4-methoxy-tremeton (2) on the basis of their spectroscopic data, respectively. All of the isolates were evaluated for their effects on the inhibition of nitric oxide (NO) production in lipopolysaccharide-activated RAW264.7 macrophages and compounds 1 and 8 exhibited inhibitory activity with IC(50) values of 35.80 and 24.41 μM, respectively. 相似文献
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Yosuke Nakamura Hisashi Miyafuji Haruo Kawamoto Shiro Saka 《Journal of Wood Science》2011,57(4):331-337
Acetic acid fermentability of various compounds from beech wood as produced in a two-step hot-compressed water treatment was
evaluated by fermentation tests using standard compounds with Clostridium thermoaceticum and Clostridium thermocellum. For cellulose- and hemicellulose-derived products, the former microorganism was found to ferment compounds with low molecular
weights such as monosaccharides, decomposed products, and organic acids to acetic acid, while the latter was found to ferment
compounds with high molecular weights such as polysaccharides and oligosaccharides to acetic acid. Lignin-derived products
were, on the other hand, fermented by both microorganisms to acetic acid. Based on these lines of evidence, co-culture with
C. thermoaceticum and C. thermocellum was evaluated and proven to increase acetic acid fermentability. Consequently, almost all compounds produced from beech wood
in hot-compressed water were found to be converted to acetic acid when using these microorganisms in combination. Thus, hot-compressed
water treatment coupled with acetic acid fermentation would likely be a powerful method to produce acetic acid from lignocellulosics. 相似文献
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濒危植物夏蜡梅叶片酚类化合物含量的动态分析 总被引：1，自引：0，他引：1
以国家2级保护植物夏蜡梅为研究对象,对自然条件下夏蜡梅叶片不同生长时期的总黄酮、总酚酸、总鞣质等3种酚类化合物含量进行了测定与分析。结果表明:夏蜡梅叶片3种酚类化合物含量随季节的不同而有差异。3种酚类化合物含量的变化曲线均为"双峰型",其中,总黄酮的含量在5月和7月出现2个峰值;总酚酸的含量在7月和9月出现2个峰值;总鞣质的含量在6月和8月出现2个峰值。3种酚类化合物总含量的变化呈现出先升高而后降低的趋势,以7月最高,10月最低。3种酚类化合物的总含量与总酚酸含量之间存在显著的相关性,与总黄酮含量之间存在极显著的相关性。 相似文献
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Two new naturally occurring formylated phloroglucinol compounds (FPCs), a dimer, loxophlebal B (10) and a cyclized FPC, loxophlebene (8) together with eight other formylated phloroglucinols (1–7 and 9) were isolated from the chloroform-methanol (8:2) extract of the leaves of Eucalyptus loxophleba ssp. lissophloia. The structures of new compounds were established by comprehensive spectral analysis and by comparison of their NMR data with those of related compounds in the literature. All the isolated compounds were evaluated for anti-leishmanial activity against promastigotes of Leishmania donovani. 相似文献