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梨中AVERMECTIN B1残留的酶联免疫吸附测定 总被引：2，自引：0，他引：2
本文建立了检测梨中Ａｖｅｒｍｅｃｔｉｎ Ｂ１的间接竞争酶联免疫吸附测定法（ＥＬＩＳＡ）。以结合物４″－Ｏ－（单）琥珀酰Ａｖｅｒｍｅｃｔｉｎ Ｂ１－ＢＳＡ免疫家兔，制备特异性针对Ａｖｅｒｍｅｃｔｉｎ Ｂ１的多克隆抗体。梨样本经甲醇提取后用ＥＬＩＳＡ检测。在６．０￣６０ｍｇ／ｋｇ添加浓度范围内，Ａｖｅｒｍｅｃｔｉｎ Ｂ１的回收率为８６％￣１０５％，变异系数（ＣＶ）为４％￣８％。样本检测限为０．１ｍｇ／ 相似文献
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本文研究了乌兰察布高原的地带性土壤府殖质的地理分布规律：（碱溶性腐殖质可提取的部分）以深厚黑色土为最高：８０－８７％；向北过渡至栗钙土、棕钙土明显降低在２０％左右，ＨＡ／ＦＡ亦是以深厚黑色土最高：土层中部达２以上；栗钙土１－１．５；至棕钙土０．６－１，并且表层最高，下部土层减少＜１；山地土壤ＨＡ／ＦＡ最高层在Ａ１层：１－１．３左右，向下锐减。光密度特性Ｅ４：Ｅ６：深厚黑色土最小＜４；草原土壤在４－５；山地土壤：＞５。土壤Ｃ／Ｎ也反映了土壤类型之间差异：深厚黑色土：表层低：６－７，下层高：１２－１３；草原土壤多在９－１０；山地土壤则较大１１－１３。通过对土壤以上各化学指标分析研究，明确了土壤类型的划分依据，对本区土壤分类中存在的几个问题进行初步探讨。 相似文献
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广西县级植保站病虫情报的技术考核王华生廖皓年（广西壮族自治区农作物病虫测报站南宁５３００２２）表１病虫情报质量等级的代码、权重、级值和级值范围（广西）代码Ａ＋ＡＡ－Ｂ＋ＢＢ－Ｃ＋ＣＣ－权重１．０００．９５０．９００．８５０．８００．７５０．７００．６... 相似文献
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利用土壤拮抗性微生物防治棉花枯萎病 总被引：13，自引：0，他引：13
从棉株根围土壤中分离到２００多株土壤微生物。通过土壤微生物－棉枯萎病菌平板对峙培养,筛选出抑菌效果较好的７个菌株,即绿色木霉Ｔ－０３,芽孢杆菌Ｂ－０１、Ｂ－０３,放线菌Ａ－０３、Ａ－０６、Ａ－０７、Ａ－１９。用上述７个土壤拮抗菌进行棉花枯萎病田间小区防效试验的结果表明,Ｔ－０３对棉花枯萎病的防效最高,达７６．５％;４个放线菌株的防病效果在６０．５％～６５．１％之间,其中Ａ－０３对棉花出苗有一定抑制作用;Ｂ－０１、Ｂ－０３的防效分别是３６．４％和５４．０％。 相似文献
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本文首次报道新疆维吾尔自治区乌鲁木齐周围地区的盘星藻属５种,４变种,其中４株,４变种为新疆新记录,这些结果是对１９９１年７月和１９９５年４－６月所采集的８７号标本鉴定后获得的。 相似文献
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乌鲁木齐地区三种不同景观鸟类群落结构初报 总被引：1，自引：0，他引：1
作者于１９９４年３－６月在乌鲁木齐地区,对三各不同景观的鸟类群落进行了调查,可划分为３类群落：干旱荒漠为角百灵＋沙群落（群落Ａ）;干旱草原为角百灵＋领岩鹨群落（群落Ｂ）;干旱灌丛云雀＋漠莺＋靴篱莺群落（群落Ｃ）群落的物种多样性以群落Ｃ最高（１．８８６７）,次为群落Ｂ（１．８８３７）,群落Ａ的多样性最低（１．５２１２）种的多样性与生态环境的差异有关。 相似文献
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三种杀真菌剂在一定浓度范围为（氯硝胺在１００×１０￣（－６），孟加拉红在５００×１０￣（－６）和柯赛得在２０００×１０￣（－６）内）对金龟子绿僵菌的菌落形成没有明显影响，而柯赛得（含５０％氢氧化铜）和孟加拉红分别在１００×１０￣（－６）和５００×１０￣（－６）浓度时显著地增加金龟子绿僵菌的产孢量，柯赛得还具有缩短产孢时间的作用。氯硝胺在１～１００×１０￣（－６）浓度范围内，随着浓度增加，对产孢能力和菌落大小的抑制作用加强。柯赛得和孟加拉红随浓度的增加对菌落的抑制作用也有所加强，但没有像氯硝胺的抑制作用那么强。 相似文献
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为明确小豆白粉病病原菌的种类以及小豆种质资源对白粉病的抗性,采用形态学和系统发育学方法对近年来在北京市发生的小豆白粉病病原菌种类进行鉴定,并采用室内苗期人工接种法评价小豆常见栽培品种（系）对白粉病的抗性。结果表明,从北京市采集的感白粉病小豆病样中培养获得病原菌BJ1,该菌能在小豆叶片和茎上产生明显的白色粉斑,分生孢子梗直立,不分枝,分生孢子单细胞,成链状着生于分生孢子梗上,呈椭圆形或卵圆形。通过rDNA-ITS序列系统发育分析,小豆白粉病菌BJ1被鉴定为白粉菌目白粉菌科的苍耳叉丝单囊壳Podosphaera xanthii。室内苗期人工接种条件下,19个供试小豆品种（系）接种小豆白粉病菌BJ1后均可发病,其中9个审定品种均表现为中度感病或高度感病,10个优良品系发病略轻。 相似文献
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为西北农林科技大学小麦新育成品种（系）在黄淮麦区的大面积推广,该研究对83份西农新育成的小麦品种（系）进行苗期抗条锈病和白粉病鉴定,成株期抗条锈病、白粉病、叶锈病和赤霉病鉴定,并在田间自然环境下对其抗性进行鉴定及对相关抗病基因进行分子检测。结果显示,在苗期人工接种鉴定中,有63、29和16份小麦品种（系）分别对条锈菌Puccinia striiformis f.sp.tritici生理小种CYR32、CYR33和CYR34表现出抗性,9份小麦品种（系）对3个条锈菌生理小种均表现出抗性;有10、3和0份小麦品种（系）分别对白粉菌Blumeria graminis f.sp.tritici生理小种E15、E09和A13表现出抗性。在成株期人工接种鉴定中,有23、15、28和62份小麦品种（系）分别对条锈病、白粉病、叶锈病和赤霉病表现出抗性。在83份小麦品种（系）中有6份在苗期和成株期均对小麦条锈病表现出抗性。在田间抗性鉴定中,有57、6、65和40份小麦品种（系）分别对条锈病、白粉病、赤霉病及叶锈病表现出抗性。在83份小麦品种（系）中,3份含有Yr5基因,22份含有Yr9基因,3份含有Yr17基因,2份含有Pm24基因,14份含有Lr1基因,所占比例分别为3.6%、26.5%、3.6%、2.4%和16.8%。 相似文献
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为明确海南省苦瓜白粉病的病原菌、生理小种及苦瓜对白粉病的抗性遗传规律,结合形态学鉴定和分子鉴定解析白粉病菌及生理小种种类,通过显微镜观察白粉病菌侵染过程,并应用主基因+多基因混合遗传模型分析法探讨苦瓜对白粉病的主要抗性遗传规律。结果表明:采集自海南省6个市(县)的苦瓜白粉病病原菌均为单囊壳白粉菌Sphaerotheca fuliginea,属生理小种2F,该菌在侵染苦瓜叶片时有4个关键时期:接种后4 h为分生孢子萌发高峰期,8 h为附着孢形成高峰期,16～24 h为次生菌丝形成高峰期,5 d为分生孢子梗形成高峰期。将其接种于苦瓜抗、感品系,对白粉病的抗性符合2对加性-显性-上位性主基因+加性-显性多基因模型,主基因和多基因共同控制苦瓜对白粉病的抗性,其中以主基因遗传为主,且会受到环境变异的影响。根据苦瓜抗性遗传规律,F2代主基因遗传率最高,受环境影响最小,在苦瓜的白粉病抗性育种中,以早期世代F2代作为有效选择世代。研究表明白粉病菌侵染叶片的前2 d是白粉病防治的最佳时期,所以在白粉病易发的物候期,可将防治时间提前1～2 d。 相似文献
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普通小麦“兰考90(6)”品系对白粉病抗性的遗传研究 总被引：7，自引：0，他引：7
普通小麦(Triticum aestivum L.)"兰考90(6)"系列品系是以六倍体小黑麦(X Triticosecale Wittmack;AABBRR)为白粉病抗源培育的新的小麦-黑麦1BL/1RS异易位系。这些品系高抗白粉病。小麦白粉病抗性基因推导试验证明,"豫麦66"携带的抗病基因与大多数已经报道的小麦抗白粉病基因不同。用白粉菌[Blumeria graminis (DC.) E. O. Speer f. sp. tritici]单孢堆分离物进行的遗传分析表明,"兰考90(6)"品系携带一个小种专化的隐性抗白粉病基因。对"中国春"和"兰考90(6)21-12"杂交F2分离群体进行1RS染色体检测,结果证明该抗白粉病基因不在1RS染色体臂上。本研究为有效利用"兰考90(6)"系列品系中的抗白粉病基因提供了科学依据。 相似文献
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陕西省小麦抗白粉病基因及新育成小麦品种(系)抗白粉病分析 总被引：6，自引：0，他引：6
为明确已知小麦抗白粉病基因载体品种以及2005—2007年陕西省新育成小麦品种(系)的抗病性,采集陕西关中不同地区小麦白粉病菌作为菌源,分年度在人工诱发病圃中对32份已知抗白粉病基因品种及578份新育成小麦品种(系)进行鉴定。结果表明,目前在陕西省仅Pm21、Pm4a、Pm5 6、Pm2 Talent及小黑麦具有优良的抗病性;Pm4b、Pm5(Mli)、Pm13、Pm19、Pm"Era"、Pm2 Mld的抗病性较弱;其它基因抗病性较差。新育成的小麦品种(系)中绝大部分对白粉病表现感病,苗期和成株期均抗病的材料仅27份,占4.67%,成株期抗病性的46份,占7.96%,其中,簇毛麦后代材料抗病性较高。 相似文献
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Progeny comprising 1643 hop seedlings from crosses between eight partially resistant parents were screened under glasshouse conditions for resistance to a single pathotype of Sphaerotheca humuli. Results indicated polygenic inheritance of resistance. Genotypes representative of seedlings with 'light', 'moderate' and 'severe' infection from each family were transplanted to a hop garden where a natural epidemic of an introduced pathotype of powdery mildew developed. There was a significant positive association between amounts of disease on seedlings and on leaves of adult male plants, but no corresponding agreement between amounts of disease on female plants. A weak positive association was found between infection of seedlings in the glasshouse and subsequent cone infection in the field. Estimates of infection on leaves of adult plants and on cones were very closely associated. Partial resistance to powdery mildew was not associated with any deleterious agronomic or brewing quality characters. 相似文献
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Thirty-nine genotypes ofCucumis melo (plant introduction entries, open-pollinated cultivars and F1 hybrids) were evaluated for resistance to powdery mildew under either natural field conditions or artificial inoculation
in growth chambers at the cotyledonary stage and the 2-true-leaf stage. Results confirmed that susceptibility in cotyledons
was not necessarily associated with susceptibility in either true leaves in growth chambers or adult plants in the field.
However, resistance at the 2-true-leaf stage in growth chambers was highly correlated with resistance of field-grown plants.
Results also showed that 20 muskmelon genotypes resistant to race 1 at the cotyledonary stage were also resistant at the 2-leaf-stage
and as adult plants in the field. The same was true for ten genotypes with race 2 inoculations. Because muskmelon genotypes
expressing resistance in cotyledons were also resistant in true leaves in growth chambers or the field, the use of plants
at the cotyledonary stage is recommended for screening for powdery mildew resistance caused by race 1 or race 2 ofS. fuliginea. When cotyledons are susceptible, screening should be done at the 2-true-leaf stage. 相似文献
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Aegilops sharonensis (Sharon goatgrass) is a wild relative of wheat and a rich source of genetic diversity for disease resistance. The objectives of this study were to determine the genetic basis of leaf rust, stem rust, and powdery mildew resistance in A. sharonensis and also the allelic relationships between genes controlling resistance to each disease. Progeny from crosses between resistant and susceptible accessions were evaluated for their disease reaction at the seedling and/or adult plant stage to determine the number and action of genes conferring resistance. Two different genes conferring resistance to leaf rust races THBJ and BBBB were identified in accessions 1644 and 603. For stem rust, the same single gene was found to confer resistance to race TTTT in accessions 1644 and 2229. Resistance to stem rust race TPMK was conferred by two genes in accessions 1644 and 603. A contingency test revealed no association between genes conferring resistance to leaf rust race THBJ and stem rust race TTTT or between genes conferring resistance to stem rust race TTTT and powdery mildew isolate UM06-01, indicating that the respective resistance genes are not linked. Three accessions (1644, 2229, and 1193) were found to carry a single gene for resistance to powdery mildew. Allelism tests revealed that the resistance gene in accession 1644 is different from the respective single genes present in either 2229 or 1193. The simple inheritance of leaf rust, stem rust, and powdery mildew resistance in A. sharonensis should simplify the transfer of resistance to wheat in wide crosses. 相似文献