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1.
Abstract

The impact of conservation tillage, crop rotation, and cover cropping on soil‐quality indicators was evaluated in a long‐term experiment for cotton. Compared to conventional‐tillage cotton, other treatments had 3.4 to 7.7 Mg ha?1 more carbon (C) over all soil depths. The particulate organic matter C (POMc) accounts for 29 to 48 and 16 to 22% of soil organic C (SOC) for the 0‐ to 3‐and 3‐ to 6‐cm depths, respectively. Tillage had a strongth influence on POMc within the 0‐ to 3‐cm depth, but cropping intensity and cover crop did not affect POMc. A large stratification for microbial biomass was observed varing from 221 to 434 and 63 to 110 mg kg?1 within depth of 0–3 and 12–24 cm respectively. The microbial biomass is a more sensitive indicator (compared to SOC) of management impacts, showing clear effect of tillage, rotation, and cropping intensity. The no‐tillage cotton double‐cropped wheat/soybean system that combined high cropping intensity and crop rotation provided the best soil quality.  相似文献   

2.
Poor soil health and low soil water content during crop growing period are major factor for low productivity of pearl millet – mustard rotation under rainfed semi-arid regions. The authors evaluated five different tillage and residue management practices for improving physico–chemical and biological properties of soil. Results showed that conservation agriculture (CA) practice (zero tillage (ZT) with 4 t ha–1 residue retention) exhibited higher proportion of soil macro-aggregate. It also increased infiltration rate of about 15.2% over conventional tillage without residue but ZT increased soil penetration resistance in surface soil layer. In the residue applied plots, ~2–4% (w/w) higher soil water content was maintained throughout the season than the no-residue plots. CA practice had the highest soil organic carbon (4.96 g kg–1) and microbial biomass carbon (188.3 μg g–1 soil). Significant and positive correlation was also found between soil organic carbon with infiltration rate (r = 0.73**), mean weight diameter (r = 0.80**) and microbial biomass carbon (r = 0.86**). Thus, this study suggests that ZT with residue retention can be advocated in pearl millet – mustard rotations for improving, productivity, soil health and maintaining higher soil water content in rainfed semi-arid regions.  相似文献   

3.
A field experiment was conducted at ICAR-Indian Institute of Sugarcane Research, Lucknow, with three tillage practices (T1: Control- two times ploughing with harrow and cultivator, each followed by planking before sugarcane planting; T2: Deep tillage with disc plough (depth 25–30 cm) before planting followed by harrowing, cultivator, and planking; and T3: Subsoiling at 45–50 cm and deep tillage with disc plough/moldboard plough (depth 25–30 cm) followed by harrowing, cultivator, and planking before planting, two soil moisture regimes (M1: 0.5 irrigation water (IW)/cumulative pan evaporation (?CPE) ratio and M2: 0.75 IW/CPE ratio) at 7.5 cm depth of IW, and four N levels (N1- 0, N2- 75, N3- 150, and N4-225 kg N ha?1) in sugarcane plant crop. Deep tillage and subsoiling increased porosity and reduced bulk density in surface/subsurface soil. Further, these physical changes also improved soil biological and chemical properties responsible for higher crop growth and yield. Deep tillage and subsoiling reduced the compaction by 6.12% in 0–15 cm depth in sugarcane plant crop at maximum tillering stage. The highest N uptake (158.5 kg ha?1) was analyzed with deep tillage and subsoiling compared to all other tillage practices. Maintaining suboptimal moisture regime with deep tillage and subsoiling showed the highest IW use efficiency (157.16 kg cane kg?1 N applied). Mean soil microbial biomass carbon (SMBC) in ratoon crop was higher compared to plant crop. During initial tillering stage, ratoon crop showed higher SMBC with application of deep tillage and subsoiling (1209 mg CO2-C g?1 soil day?1) at 0–15 cm depth and 1082.9 mg CO2-C g?1 soil day?1 at 15–30 cm depth. Thus, it could be concluded that besides improving sugarcane yield, soil health could be sustained by adopting subsoiling (45–50 cm depth) and deep tillage (20–25 cm depth), with soil moisture regime of 0.75 IW/CPE and application of 150 kg N ha?1 in sugarcane (plant crop).  相似文献   

4.
This study evaluates soil properties in organically managed olive groves and natural zones in a mountainous area of Andalusia, Spain. Two soil types (Eutric Regosol and Eutric Cambisol) and the most common soil management methods (tillage and two intensities of grazing) were studied. Both soil types in the groves had values not much lower than those in the natural areas. Average (±SE) values in the groves were 1.58 ± 0.71% for organic carbon, 323 ± 98 g kg?1 for macroaggregate stability, 1.11 ± 0.16 g cm?3 for bulk density, 3.5 ± 1.6 mm h?1 for saturated hydraulic conductivity and 1209 ± 716 mg CO2 kg?1 for soil respiration. Overall, these values tended to be lower in the tilled compared with that in the grazed groves. The average phosphorus soil content (5.83 ± 5.22 mg kg?1) was low for olive production and within adequate ranges for N (0.12 ± 0.05%) and K (142 ± 81 mg kg?1). Soil erosion was high in the tilled groves (35.5 ± 18.2 t ha?1 year?1) with soil loss correlating with indicators of soil degradation such as organic carbon content and water stable macroaggregates. In the grazed groves, soil loss was moderate with no clear indications of soil degradation. Overall, there was significant farm‐to‐farm variability within the same soil and land management systems. Olive production had a moderate effect on soil degradation compared with natural areas and olive cultivation could be sustained in future if cover crop soil management replaced tillage, especially in the most sloping areas.  相似文献   

5.
The influence of differing soil management practices on changes seen in soil organic carbon (SOC) content of loamy Haplic Luvisol was evaluated. The field experiment included two types of soil tillage: 1. conventional tillage (CT) and 2. reduced tillage (RT) and two treatments of fertilization: 1. crop residues with nitrogen, phosphorus, and potassium (NPK) fertilizers (PR+NPK) and 2. NPK fertilizers (NPK). The results of SOC fluctuated from 9.8 to 14.5 g kg?1 and the tillage systems employed and fertilization status did not have a statistically significant influence on SOC. The SOC content was higher in RT (12.4 ± 0.86 g kg?1) than in CT (12.2 ± 0.90 g kg?1). On average, there was a smaller higher value of SOC in PR+NPK (12.4 ± 1.02 g kg?1) than in NPK (12.3 ± 0.88 g kg?1). During a period of 18 years, reduced tillage and application of NPK fertilizers together with crop residues build up a SOC at an average speed of 7 and 16 mg kg?1 year?1, respectively, however conventional tillage and NPK fertilizer applications caused a SOC decline at an average speed of 104 and 40 mg kg?1 year?1, respectively.  相似文献   

6.
Vineyard management practices to enhance soil conservation principally focus on increasing carbon (C) input, whereas mitigating impacts of disturbance through reduced tillage has been rarely considered. Furthermore, information is lacking on the effects of soil management practices adopted in the under-vine zone on soil conservation. In this work, we evaluated the long-term effects (22 years) of alley with a sown cover crop and no-tillage (S + NT), alley with a sown cover crop and tillage (S + T), and under-vine zone with no vegetation and tillage (UV) on soil organic matter (SOM), microbial activity, aggregate stability, and their mutual interactions in a California vineyard in USA. Vegetation biomass, microbial biomass and activity, organic C and nitrogen (N) pools, and SOM size fractionation and aggregate stability were analysed. Soil characteristics only partially reflected the differences in vegetation biomass input. Organic C and N pools and microbial biomass/activity in S + NT were higher than those in S + T, while the values in UV were intermediate between the other two treatments. Furthermore, S + NT also exhibited higher particulate organic matter C in soil. No differences were found in POM C between S + T and UV, but the POM fraction in S + T was characterized by fresher material. Aggregate stability was decreased in the order: S + NT > UV > S + T. Tillage, even if shallow and performed infrequently, had a negative effect on organic C and N pools and aggregate stability. Consequently, the combination of a sown cover crop and reduced tillage still limited SOM accumulation and reduced aggregate stability in the surface soil layer of vineyards, suggesting relatively lower resistance of soils to erosion compared to no-till systems.  相似文献   

7.
One way to increase the amount of carbon sequestered in agricultural land is to convert conventional tillage into no‐tillage systems. This greatly affects the location of crop residues in soil. To investigate the impact of the location of residues on soil physical and biological properties and how the interactions between those properties influence the fate of carbon and nitrogen in soil, we did a laboratory experiment with repacked soil in columns. Doubly labelled 13C15N oilseed rape residues were incorporated in the 0–10 cm layer or left on the soil surface. The columns were incubated for 9 weeks at 20°C and were submitted to three cycles of drying and wetting, each of them induced by a rain simulator. The location of the residues affected the water dynamics and the distribution of C and N in the soil, which in turn influenced microbial activity and the decomposition rate of the added residues. After 9 weeks of’incubation, 18.4 ± 1.5% of the surface applied residue‐C and 54.7 ± 1.3% of the incorporated residue‐C was mineralized. We observed a nitrate accumulation of 10.7 mg N kg?1 with residues at the soil surface, 3.6 mg N kg?1 with incorporated residues and 6.3 mg N kg?1 without addition of fresh organic matter, which entailed net N mineralization in soil under mulch and immobilization of N with residue incorporation compared with the control soil. We concluded that application of oilseed rape residues at the soil surface increased the storage of fresh organic C in soil in the short term, compared with the incorporation treatment, but increased the risk of nitrate leaching.  相似文献   

8.
Maintaining soil organic carbon (SOC) in arid ecosystem is important for soil productivity and restoration of deserted sandy soil in western plain of India. There is a need to understand how the cropping systems changes may alter SOC pools including total organic carbon (TOC), particulate organic C (POC), water soluble carbon (WSC), very labile C (VLC), labile C (LC), less labile C (LLC) and non-labile C (NLC) in arid climate. We selected seven major agricultural systems for this study viz., barren, fallow, barley–fallow, mustard–moth bean, chickpea–groundnut, wheat–green gram and wheat–pearl millet. Result revealed that conversion of sandy barren lands to agricultural systems significantly increased available nutrients and SOC pools. Among all studied cropping systems, the highest values of TOC (6.12 g kg?1), POC (1.53 g kg?1) and WSC (0.19 g kg?1) were maintained in pearl millet–wheat system, while the lowest values of carbon pools observed in fallow and barren land. Strong relationships (P < 0.05) were exhibited between VLC and LC with available nutrients. The highest carbon management index (299) indicates that wheat–pearl millet system has greater soil quality for enhancing crop productivity, nutrient availability and carbon sequestration of arid soil.  相似文献   

9.
耕作与施肥对甘蔗地土壤微生物量碳、氮的影响   总被引：2，自引：0，他引：2  
何瑞清  王百群    张燕 《水土保持研究》2015,22(5):118-121
通过野外调查与室内分析,研究了云南蒲缥甘蔗地赤红壤不同耕作与施肥土壤剖面微生物量碳、氮特征。结果表明:不同耕作与施肥对土壤微生物量碳、氮均有一定影响,其中耕作对土壤微生物量碳、氮的影响较为一致,表现为免耕 > 翻耕。施肥对0—20 cm土层中微生物量碳的影响表现为翻耕施肥 > 翻耕,对20—40 cm,40—60 cm土层中微生物量碳的效应呈现为翻耕 > 翻耕施肥,而施肥对土壤微生物量氮影响则与其对微生物量碳的效应相反。耕作和施肥对各个土层土壤微生物商具有显著的影响（p<0.05）,表现为免耕 > 翻耕,翻耕施肥条件下0—20 cm土层的微生物商稍高于翻耕,而其他土层均为翻耕 > 翻耕施肥;土壤微生物量氮与全氮的比值与微生物量氮的变化趋势相近。在不同的耕作和施肥条件下,免耕有利于提高土壤微生物量,施肥在一定程度上也利于提高土壤微生物量。  相似文献   

10.
The farming practices in vineyards vary widely, but how does this affect vineyard soils? The main objective of this study was to evaluate the effects of vineyard management practices on soil organic matter and the soil microbial community. To this end, we investigated three adjacent vineyards in the Traisen valley, Austria, of which the soils had developed on the same parent material and under identical environmental/site conditions but were managed differently (esp. tillage, fertilizer application, cover crops) for more than 10 yrs. We found that topsoil bulk density (BD) decreased with increasing tillage intensity, while subsoil BD showed the opposite trend. Soil organic carbon (SOC) stocks in 0–50 cm depth increased from 10 kg m?2 in an unfertilized and frequently tilled vineyard to 17 kg m?2 in a regularly fertilized but less intensively tilled vineyard. Topsoil microbial biomass per unit SOC, estimated by the sum of microbial phospholipid fatty acids (PLFAs), followed this trend, albeit not statistically significantly. Principal component analysis of PLFA patterns revealed that the microbial communities were compositionally distinct between different management practices. The fungal PLFA marker 18:2ω6,9 was highest in the vineyard with the lowest amount of extractable Cu (by 0.01 m CaCl2), and the bacterial‐to‐fungal biomass ratio was positively correlated with extractable Cu. Our results indicate that tillage and fertilizer application of vineyards can strongly affect vineyard soil properties such as BD and SOC stocks and that the application of Cu‐based fungicides may impair soil fungal communities.  相似文献   

11.
Tropical legume cover crops are important components in cropping systems because of their role in improving soil quality. Information is limited on the influence of nitrogen (N) fertilization on growth of tropical legume cover crops grown on Oxisols. A greenhouse experiment was conducted to evaluate the influence of N fertilization with or without rhizobial inoculation on growth and shoot efficiency index of 10 important tropical cover crops. Nitrogen treatment were (i) 0 mg N kg?1 (control or N0), (ii) 0 mg N kg?1 + inoculation with Bradyrhizobial strains (N1), (iii) 100 mg N kg?1 + inoculation with Bradyrhizobial strains (N2), and (iv) 200 mg N kg?1 of soil (N3). The N?×?cover crops interactions were significant for shoot dry weight, root dry weight, maximal root length, and specific root length, indicating that cover crop performance varied with varying N rates and inoculation treatments. Shoot dry weight is considered an important growth trait in cover crops and, overall, maximal shoot dry weight was produced at 100 mg N kg?1 + inoculation treatment. Based on shoot dry-weight efficiency index, cover crops were classified as efficient, moderately efficient, and inefficient in N-use efficiency. Overall, the efficient cover crops were lablab, gray velvet bean, jack bean, and black velvet bean and inefficient cover crops were pueraria, calopo, crotalaria, smooth crotalaria, and showy crotalaria. Pigeonpea was classified as moderately efficient in producing shoot dry weight.  相似文献   

12.
不同耕作方式下玉米农田土壤养分及土壤微生物活性变化   总被引：4，自引：0，他引：4  
通过连续3年的野外调查与室内分析试验,研究了不同耕作方式（翻耕、旋耕、免耕）对玉米农田土壤养分及土壤微生物活性的影响。结果表明,不同耕作方式下土壤pH值略显酸性,土壤容重与土壤总孔隙度变化趋势相反;土壤容重基本表现为:免耕 > 翻耕 > 旋耕;土壤总孔隙度TSP基本表现为:翻耕 > 旋耕 > 免耕,不同耕作方式差异均显著（p<0.05）。不同耕作方式下土壤养分（有机碳、全氮含量）和有效养分（有效磷、铵态氮和硝态氮）均呈现出一致性规律,大致表现为翻耕 > 旋耕 > 免耕,不同耕作方式下土壤全磷含量差异均不显著（p > 0.05）;与免耕相比,土壤微生物量碳和氮、土壤微生物数量（细菌、真菌、放线菌、固氮菌和纤维素菌）均有明显的增加,大致表现为:翻耕 > 旋耕 > 免耕。土壤微生物活度的变化范围为0.38~0.69,依次表现为翻耕 > 旋耕 > 免耕,不同耕作方式下土壤微生物活度差异均显著（p<0.05）。不同耕作方式下土壤微生物量碳周转率高于氮周转率,说明微生物量碳更新比微生物量氮快,其中翻耕处理下土壤微生物量碳和氮更新较旋耕和免耕快。通径分析发现,不同土壤环境因子对土壤微生物活度产生直接和间接负作用,其中有机碳、全氮、硝态氮、铵态氮、细菌数量对土壤微生物活度产生直接效应;土壤微生物量碳、微生物量氮、真菌数量和固氮菌数量对土壤微生物活度产生间接效应。  相似文献   

13.
A study was conducted to examine the impact of land use on soil fertility in an Entisol in the Jalpaiguri District of humid subtropical India. The natural forest served as a control against which changes in soil properties were compared. Soil samples were collected from four different depths (0–25, 25–50, 50–75, and 75–100 cm) of soil from four land uses (viz. forest, home garden, arecanut plantation, and agriculture) and examined for pH, organic carbon (OC), electrical conductivity (EC), cation exchange capacity, available nitrogen (N), phosphorus (P), exchangeable calcium (Ca), magnesium (Mg), potassium (K), aluminum (Al), microbial biomass carbon (MBC), microbial biomass nitrogen (MBN) and dehydrogenase activity (DHA). Soil pH (5.7), OC (2.29%), N (386 kg ha?1), and P (22.54 kg ha?1) were greatest in forest soil, followed by soil from arecanut plantation, agriculture, and home garden. The greatest Ca (0.892 cmol kg?1), Mg (0.527 cmol kg?1), and Al (1.86 cmol kg?1) were found in the arecanut plantation, whereas K (0.211 cmol kg?1) was greatest in forest. The greatest content of diethylenetriaminepentaacetic acid–extractable copper, zinc, manganese, and iron (2.25, 1.66, 4.86, and 7.65 ppm, respectively) were found in forest. MBC (558 mg kg?1), MBN (26.67 mg kg?1), and DHA (33.03 μg TPF 24 h?1 g?1) was greatest in forest soil. Soil fertility index varied from 13.13 in arecanut plantation to 18.49 in forest. The soil evaluation factor ranged from 5.32 in agriculture to 6.56 in forest. Pearson's correlation matrix revealed strongly significant positive correlation of soil fertility index and soil evaluation factor with soil properties.  相似文献   

14.
不同保护性耕作措施对麦-豆轮作土壤有机碳库的影响   总被引：13，自引：3，他引：13  
通过设置在甘肃省定西市李家堡镇的不同保护性耕作试验,对春小麦、豌豆两种轮作次序下的土壤总有机碳、活性有机碳、微生物量碳含量进行了测定,并计算了各处理土壤碳库管理指数.结果表明:经过5年的轮作后,与传统耕作相比,两种轮作次序下免耕秸秆覆盖和传统耕作结合秸秆还田处理均能不同程度地提高土壤总有机碳、活性有机碳、微生物量碳含量及土壤碳库管理指数,而免耕不覆盖处理除在0～5 cm提高了土壤有机碳库管理指数外,其他各层次均降低了土壤有机碳库管理指数,说明仅依靠免耕而不结合秸秆覆盖或还田对于土壤有机碳库的管理来讲是不可持续的.  相似文献   

15.
[目的]覆盖作物影响果园土壤的微生物和线虫群落,研究不同覆盖作物对土壤微生物和线虫群落的影响特征可为生态果园管理提供理论依据.[方法]试验于2016年在湖北十堰的猕猴桃园内进行,供试品种为美味猕猴桃(Actinidia deliciosa),2015年定植.覆盖作物处理为白三叶草、鼠茅草,以清耕为对照(CK).连续进行...  相似文献   

16.
The effects of different integrated farming systems on microbial biomass was studied 20 years after their adoption at Meghalaya, India. The soil fertility was relatively greater in agricultural and agri‐horti‐silvi‐pastoral systems as a result of accumulation of leaf litter/crop residues and addition of inorganic and organic manures. Microbial biomass carbon was greatest in agricultural (378 mg kg?1) followed by the agri‐horti‐silvi‐pastoral systems (291 mg kg?1). The most microbial biomass nitrogen (N) and phosphorus (P) (32.4 and 17.07 mg kg?1, respectively) were recorded in agricultural followed by agri‐horti‐silvi‐pastoral systems. Microbial biomass carbon (C) had a significant relationship with organic C, microbial biomass N, and biomass P, indicating that the living part of soil organic matter is involved in the transformation of nutrients into the labile pool and governs their availability to the plants. Application of inorganic fertilizers and organics along with lime has contributed more microbial biomass that led to more biological activity attributed in nutrient transformations and also maintained the soil fertility.  相似文献   

17.
Abstract

Soil carbon sequestration in agricultural lands has been deemed a sustainable option to mitigate rising atmospheric CO2 levels. In this context, the effects of different tillage and C input management (residue management and manure application) practices on crop yields, residue C and annual changes in total soil organic C (SOC) (0–30 cm depth) were investigated over one cycle of a 4-year crop rotation (2003–2006) on a cropped Andisol in northern Japan. For tillage practices, the effects of reduced tillage (no deep plowing, a single shallow harrowing for seedbed preparation [RT]) and conventional deep moldboard plow tillage (CT) were compared. The combination of RT, residue return and manure application (20 Mg ha?1 in each year) increased spring wheat and potato yields significantly; however, soybean and sugar beet yields were not influenced by tillage practices. For all crops studied, manure application enhanced the production of above-ground residue C. Thus, manure application served not only as a direct input of C to the soil, but the greater crop biomass production engendered enhanced subsequent C inputs to the soil from residues. The SOC contents in both the 0–5 cm and 5–10 cm layers of the soil profile were greater under RT than under CT treatments because the crop residue and manure were densely incorporated into the shallow soil layers. Comparatively, neither tillage nor C input management practices had significant effects on annual changes in SOC content in either the 10–20 cm or 20–30 cm layers of the soil profile. When soil C sequestration rates, as represented by annual changes in total SOC (0–30 cm), were assessed on a total soil mass basis, an anova showed that tillage practices had no significant effect on total C sequestration, but C input management practices had significant positive effects (P ≤ 0.05). These results indicate that continuous C input to the soil through crop residue return and manure application is a crucial practice for enhancing crop yields and soil C sequestration in the Andisol region of northern Japan.  相似文献   

18.
Summary The soil microbial biomass contains important labile pools of C, N, P, and S, and fluctuations in its size and activity can significantly influence crop productivity. In cropping systems where fertilizer use is reduced or eliminated and green-manure legumes are used, nutrient availability is more directly linked to C-cycle dynamics. We observed the fluctuations in microbial biomass C and P, and in microbial biomass activity over three cropping seasons in continuous maize and 2-year maize-wheat-soybean rotation agroecosystems under no-till and reduced-chemical-input management. We estimated the concentrations of microbial C and P using fumigation-incubation and fumigation-extraction techniques for the surface 20 cm of Cecil and Appling series soils (clayey, kaolinitic, thermic, Typic Kanhapludults). There were significant seasonal fluctuations in microbial C and P under all cropping systems. Generally, the magnitude of fluxes and the quantity of microbial C and P tended to be higher in reduced-chemical-input systems due to tillage and incorporation of crop, weed, and legume residues. Over 3 years, the means for microbial C were 435 under reduced-input maize; 289 under no-till maize; 374 und the reduced-input crop rotation; and 288 mg kg-1 soil under the no-till rotation. The means for microbial P were 5.2 under reduced-input maize; 3.5 under no-till maize; 5.0 under the reduced-input rotation; and 3.5 mg kg-1 soil under the no-till rotation. Estimates of microbial activity, derived from CO2–C evolution and specific respiratory activity (mg CO2–C per mg biomass C), suggest that reduced-chemical-input management may cause a larger fraction of the biomass to be relatively inactive but may also increase the activity of the remaining fraction over that in no-till. Thus in these specific systems, the turnover of C and P through the microbial biomass with a reduced chemical input to the soil may be higher than under a no-till system.  相似文献   

19.
Although tropical and subtropical environments permit two cropping cycles per year, maintaining adequate mulching on the soil surface remains a challenge. In some cases, leaving soils fallow during the winter as an agricultural practice to control pathogens contributes to reduce soil mulching. The aim of this study was to assess attributes associated with C and N cycling in a soil under conventional and no-tillage management, with contrasting uses in winter: black oats (Avena strigosa Schreb) as cover crop or fallow. No-tillage increased total C and N, irrespective the winter crop. Cropping black oats under no-tillage resulted in more microbial biomass C and N, and glutaminase activity (15.2%, 65.2%, and 24%, respectively) than no-tillage under fallow. Under conventional tillage, winter cropping did not affect the attributes under study. Available P was higher in the no-tillage system (9.2–12.3 mg kg−1), especially when cropped with black oats, than in the conventional tillage system (4.8–6.6 mg kg−1). A multivariate analysis showed strong relationships between soil microbiological and chemical attributes in the no-tillage system, especially when cropped with black oats. Soil pH, dehydrogenase and acid phosphatase activities were the most effective at separating the soil use in winter. Microbial N, total N, microbial to total N ratio, available P, metabolic quotient (qCO2), and glutaminase activity were more effective at separating soil management regimes. The no-tillage system in association with winter oat cropping stimulated the soil microbial community, carbon and nutrient cycling, thereby helping to improve the sustainability of the cropping system.  相似文献   

20.
The objective of the present study was to increase understanding of the effects of heavy metal pollution and soil properties on microorganisms in relation to the biomass and microbial functional community. Soil samples were collected from aged polluted and reference sites on a floodplain. The soil Cu, Zn and Pb total concentrations were much higher at the polluted sites (average 231.6–309.9 mg kg?1, 195.7–233.0 mg kg?1, and 72.4–86.0 mg kg?1, respectively) than at the reference site (average 33.3–44.0 mg kg?1, 76.7–98.0 mg kg?1, and 30.8–41.6 mg kg?1, respectively), while the available heavy metal concentrations in CaCl2 extraction were similar in all sites. Small seasonal variations in the size of microbial biomass were observed. Ambient soil properties (e.g. total C, N, pH, moisture content, and CEC) affected the soil microbial biomass more than the heavy metal pollution. However, the aged pollution tended to impact on the composition of the microbial community. PICT (pollution-induced community tolerance) test using BIOLOG Ecoplates showed enhanced tolerance of the microbial community to Cu stress in the polluted site. In non polluted but low nutrient, low pH and low moisture soil, the microbial biomass was lower and the microbial community was more vulnerable to Cu stress. In spite of the low heavy metal availability due to ageing, the BIOLOG technique provided sensitive detection of microbial community level changes in PICT analysis.  相似文献   

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