共查询到19条相似文献，搜索用时 375 毫秒
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基因水稻培矮64S回交后代白叶枯病抗性与育性研究 总被引：6，自引：2，他引：4
对筛选到的转[WTBX][STBX]Xa21[WTBZ][STBZ]基因培矮64S后代纯合株系抗性与育性的研究表明,在第5代杂种中,该外源基因仍能稳定遗传表达;以其作父本、培矮64S为母本所配制的F1植株, Xa21基因PCR检测均呈阳性,且绝大多数表现抗白叶枯病,说明该外源基因能够通过常规杂交方式转移利用。这些杂交组合F2群体中抗病与感病株出现3∶1分离,表明转Xa21基因在杂交后代的传递属单基因显性遗传。长日高温下,（培矮64S/转Xa21基因培矮64S）F1雄性完全可育,F2群体可育与不育株出现27∶1分离。 相似文献
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藉人工气候室的人控光温条件 ,对两系杂交水稻不育系培矮 64S在 2 2 .0～ 2 3 .0℃的低温下的育性表达规律进行了连续 2年的跟踪研究。主要结果如下 :1) 2 3 .0℃的低温仅能导致培矮 64S花粉不育度的明显降低 ,而 2 2 .5℃和 2 2 .0℃的低温则不仅能导致花粉不育度的下降 ,而且还能诱导一定程度的自交结实 ;2 )培矮 64S的育性温度敏感终止期为幼穗分化第 6期 (花粉母细胞减数分裂期 ) ,育性温度最敏感期为幼穗分化第 4～ 5期 (雌雄蕊形成期至花粉母细胞形成期 ) ;3 )培矮 64S具有光敏性 ,其临界光长处于 13 5 0～ 13 .3 8h之间 ,光敏温度范围为 2 3～ 2 8℃ ;4 )对于农艺性状已经稳定的高世代培矮 64S而言 ,其控制育性表达的遗传基础尚不纯 ,表现在来源相同的不同株系间或同一株系内不同单株间在低温下育性表达存在显著的差异 ,且低温强度加大 ,这种差异就更为明显 相似文献
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《杂交水稻》1990,(6)
1990年8月 24-25日及9月6日由袁隆平主任组织本中心14名中、青年育种家及管理人员,对该中心选育的两个光敏核不育 系培矮64s、 CY8526s及一个三系不育系Ⅱ-32A进行了鉴定。 培矮64s是副研究员罗孝和等同志于1985年用带光敏核不育基因的农垦58原始株与带广亲和基因的培矮64杂交,经隔代回交选育而成。经田间1150株群体鉴定,农艺性状整齐一致,不育株率为100%,不育度经镜检795杂颖花,正常花粉率为0.008%,染败花粉率为0.078%,典败花粉率为99.8345%,检查套袋250穗,自交结实率为0。该不育系稳定不育期在一个月以上,可育期自交结实率在30%以上,异… 相似文献
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籼型两用核不育系水稻培矮64S的育性表达——幼穗发育过程中的NAD+-MDH和AP同工酶的变化 总被引：2，自引：0，他引：2
以籼型两用不育系水稻培矮64S和常规稻粳籼89为材料，采用PAGE分析同工酶的方法，比较水稻幼穗发育过程中花粉母细胞形成期、减数分裂期和花粉完熟期NAD+-MDH和AP同工酶的变化，以期了解不育系的育性转换与表达机理。结果表明，在1993年2月25日、3月10日和3月30日三期播种的水稻培矮64S均表现不育，花粉不育度在99.7%以上，结实率在0.3%以下，1994年8月1日播种的培矮64S表现可育，结实率在19%以上，而同期播种的常规稻粳籼89均能得到正常结实，结实率在68%以上。同时发现1993年培矮64S的不育性在花粉完熟期与颖花及花药的NAD+-MDH活性的显著降低关系较大。在花粉母细胞形成期—减数分裂期与颖花AP的活性及同工酶组成的变化关系较密切。1994年表现可育的培矮64S与粳籼89相比不存在这种差异。由此可认为培矮64S育性表达可能与花粉发育前期的脂肪代谢以及花粉发育后期的呼吸代谢有关。 相似文献
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水稻实用光温敏核不育系培矮64S不育性稳定化研究 总被引：16，自引：1，他引：16
在人工气候室的人控光温条件下，通过1997～1999年3年22.0～23.0℃的低温处理，对水稻实用光温敏核不育系培矮64S进行了育性稳定化研究。结果表明：1)在人工气候室的低温条件下，采用“株系(再)鉴定、(再)筛选单株”的方法，对稳定高世代光温敏核不育系培矮64S的不育性是可行的，并筛选出套袋自交结实率和花粉不育度两级水平均已稳定的3个株系9862、103-1和103-2，而且其不育起点温度已降至22.5℃，比原始株系降低了近1℃。2)现有高世代光温敏核不育系培矮64S仍然存在育性的不稳定性，表现在受低温影响后其后代不同株系间花粉不育度和套袋自交结实率存在明显差异，说明对现有高世代光温敏核不育系的育性提纯很有必要。 相似文献
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WANG Cai-lin ZHAO Ling ZHU Zhen ZHANG Ya-dong 《水稻科学》2007,14(1):1-6
In order to improve the panicle extrusion of photo-and thermo-sensitive sterile line'Pei'ai 64S'by using elongated uppermost internode(eui)gene of the wide compatibility rice mutant'02428h',a new photo-and thermo-sensitive sterile line'P8hS' characterized with elongated uppermost internode was developed by transferring the eui gene into Pei'ai 64S through three successive backcrossing.Compared with Pei'ai 64S,the plant height of P8hS was 35.6 cm higher resulted from the elongation of the uppermost and the second internodes from the top.The panicle extrusion of Pei'ai 64S was completely improved and positive effects were found on the main economic characters of P8hS and its hybrids by introducing eui gene into Pei'ai 64S. 相似文献
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Changxuan 3S, a thermo-sensitive genic male sterile (TGMS) rice line with eui gene, is derived from the TGMS rice line Pei'ai 64S by irradiation with 350 Gy of 60Co y-ray. To elucidate the uppermost internode elongation of the TGMS line with eui gene, Changxuan 3S and its parent Pei'ai 64S were used to study the effects of temperature on panicle exsertion. At 24℃, the uppermost intemode of Changxuan 3S elongated the fastest from the 4thday before flowering to 0 day (flowering), being 2.1-fold as that of Pei'ai 64S, whereas it elongated slowly during the 12thday to the 4thday before flowering and the 1st to the 3rd day after flowering. The uppermost internode of Changxuan 3S exserted from the flag leaf sheath at 22℃, 24℃ and 26℃, and the length of elongated uppermost intemode increased with the decreasing temperatures. At 28℃, though the panicles of Changxuan 3S were still enclosed in the leaf sheath, the degree of panicle enclosure was significantly lower compared with Pei'ai 64S. Cytological studies on Changxuan 3S showed that the uppermost intemode elongation was attributed to the increase of cell number and cell elongation, and the latter was more significant. Moreover, the numbers of outermost and innermost parenchyma cells and the cell length of the uppermost internode reduced with the increasing temperatures. 相似文献
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Improving Blast Resistance of a Thermo-Sensitive Genic Male Sterile Rice Line GD-8S by Molecular Marker-Assisted Selection 总被引：1，自引：1，他引：0
LIU Wu-ge # JIN Su-juan ZHU Xiao-yuan WANG Feng LI Jin-hua LIU Zhen-rong LIAO Yi-long ZHU Man-shan HUANG Hui-jun LIU Yi-bai 《水稻科学》2008,15(3):179-185
The broad-spectrum blast resistance gene Pi-1, from donor line BL122, was introduced into a thermo-sensitive genic male sterile rice line GD-8S, which possessed good grain quality but high susceptibility to rice blast, by using backcross breeding and molecular marker-assisted selection. Five elite improved male sterile lines, RGD8S-1, RGD8S-2, RGD8S-3, RGD8S-4 and RGD8S-5, were selected based on the results of molecular marker analysis, spikelet sterility, recovery rate of genetic background and agronomic traits. Thirty-three representative blast isolates collected from Guangdong Province,China were used to inoculate the improved lines and the original line GD-8S artificially. The resistance frequencies of the improved lines ranged from 76.47% to 100%, much higher than that of the original line GD-8S (9.09%). On the agronomic characters, there were no significant differences between the improved lines and GD-8S except for flag leaf length and panicle number per plant. The improved lines could be used for breeding hybrid rice with high blast resistance. 相似文献