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【目的】 研究感染鲤浮肿病毒（Carp edema virus,CEV）镜鲤的组织病理特征及CEV在各组织中的分布规律,以期为鲤浮肿病的诊断和防控提供参考。【方法】 利用HE染色观察发病镜鲤心脏、肝脏、皮肤、脾脏、肾脏、脑、鳃组织的病理变化情况;用免疫组织化学法检测CEV在镜鲤各组织的分布和蛋白表达情况;利用CEV P4a基因保守区域设计3条探针,于5'-端标记FAM进行原位杂交,检测CEV在镜鲤各组织的核酸分布情况;实时荧光定量PCR检测感染镜鲤各组织的病毒载量。【结果】 HE染色观察发现,患病镜鲤的鳃丝肿胀、充血,鳃丝间有脱落堆积的红细胞和炎性细胞,肝脏细胞深染萎缩,胆小管出血,脾脏组织出现明显间隙并伴有出血,肾脏组织充血明显,肾小管有明显闭合现象。免疫组织化学结果显示,在患病鱼的鳃和肝脏组织中大量表达CEV P4a蛋白,在其他组织中也有少量表达。原位杂交结果显示,CEV核酸大量存在于鳃组织中,在心脏、脾脏和肾脏中均有少量阳性信号。实时荧光定量PCR结果显示,鳃组织病毒载量极显著高于心脏、肝脏、皮肤、脾脏、肾脏、脑组织（P<0.01）。【结论】 感染CEV后,镜鲤内脏组织出现不同程度充血、出血,病毒主要分布在鳃,说明鳃是CEV增殖的最主要靶器官。 相似文献
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内参基因的正确选择是获得精准、可靠RT-qPCR数据的重要前提。本研究随机选取3只28日龄的白羽王鸽,采集心、肝、脾、肺、肾、胸肌、腿肌、腹脂、卵巢9个组织作为实验材料,选取GAPDH、β-actin、18S rRNA和RPS24个常见的内参基因作为候选内参基因。利用RT-qPCR技术和geNorm、NormFinder、BestKeeper软件及Ct值分析法对候选基因在不同组织中的表达进行检测和稳定性分析。结果表明:geNorm、NormFinder软件分析与Ct值分析法的结果一致,即18S rRNA基因稳定性最佳,RPS2次之;BestKeeper软件的分析结果显示RPS2基因表达稳定性最好,18S rRNA基因次之。因此,推荐选择RPS2+18S rRNA的内参基因组合用于白羽王鸽不同组织基因表达的研究。 相似文献
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番鸭呼肠孤病毒病雏番鸭实质器官的超微结构 总被引：2，自引：0，他引：2
对人工感染番鸭呼肠孤病毒发病雏番鸭的心、肝、肺、肾、脾脏、胸腺、法氏囊等7种实质器官的超微结构进行了观察。电镜下发现：心、肝、肺、肾等实质器官出现不同程度的细胞变性、水肿以及局灶性溶解坏死；各器官血管内皮细胞脂滴增多、水肿以至坏死脱落．通透性增加；浆细胞、淋巴细胞和吞噬细胞呈散在或灶性浸润于坏死区和实质细胞间。免疫器官脾脏、胸腺和法氏囊中的部分淋巴细胞、浆细胞坏死和不同程度凋亡，且细胞溶解坏死形成大小不一的坏死灶并被大量增生的吞噬细胞所吞噬，淋巴细胞数量明显减少。上述结果提示，番鸭呼肠孤病毒能导致番鸭免疫抑制。 相似文献
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Takami S Goryo M Masegi T Okada K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2005,67(1):13-18
The major organs and tissues of 24 broiler chickens (70 or 71 days old) suspected of spindle-cell proliferative disease (SPD) because of showing the tumorous lesions distributed throughout the body at meat inspection were collected for histopathological and immunohistochemical examination. Macroscopically, liver, spleen and cecal tonsil showed severe enlargement and white nodules or plaques were observed in the liver, spleen, kidney, intestine and bone marrow of the femur. All chickens were diagnosed with SPD based on the histopathological examination. The lesions of SPD were observed in the liver, spleen, kidney, heart, lung, pancreas, proventriculus, gizzard, duodenum, jejunum, ileum, rectum, cecal tonsil, bursa of Fabricius, bone marrow of the femur and skin. Hemangioma was observed in the lung of 1 bird. Eight 1-day-old specific pathogen-free chicks were inoculated intraperitoneally with 0.25 ml of a 20% homogenate of the affected spleens of three naturally occurring cases. One inoculated bird, necropsied at 10 weeks of age, macroscopically had a white nodule in the kidney and histopathologically had spindle-cell proliferative lesions, a pattern similar to that seen in the naturally occurring cases, in the liver, spleen, kidney, heart, lung, pancreas, proventriculus, duodenum, cecal tonsil and bone marrow of the femur, and was diagnosed with SPD. Immunohistochemically, significant positive reactions with a rabbit antiserum against avian leukosis virus antigens were detected in all spindle cells in the proliferative lesions of all examined SPD cases and in tumor cells of the hemangioma of a field case. 相似文献
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Ohba T Shibahara T Kobayashi H Kubo M Takashima A Imai S Murakami S Kadota K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(4):449-453
A 6-month-old barrow presented with lethargy, inappetence and dysstasia. At necropsy, multiple coalescing hemorrhagic foci were detected in the margins of the spleen. Gram-positive bacilli were isolated from the spleen, kidney, muscle and liver. Comparative 16S rDNA gene sequencing analysis of the isolates (TO16177) revealed that they would be the same species of unpublished Arcanobacterium species strain HJ57-14E (accession no. gi 18873551) (99.7% similarity based on a comparison of 675 bp). Histologic examination of the splenic tissue sections revealed extensive necrosis and inflammation, and gram-positive bacilli were discernible. Multifocal necrosis was also detected in the liver. Immunohistochemically, the isolates were cross-reacted with polyclonal antibodies against Arcanobacterium pyogenes and Actinomyces naeslundii, and the reaction was strong for the latter. Similar reactions were found in the suppurative lesions of the tonsil, and occasionally in the spleen and lymph nodes. The present results indicate that the unpublished Arcanobacterium species induced multiple organ failure accompanied by acute hemorrhagic necrotizing splenitis in this growing-finishing pig. 相似文献
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Haematopoietic necrosis in a goldfish (Carassius auratus) associated with an agent morphologically similar to herpesvirus 总被引：1，自引：0，他引：1
One of 14 goldfish (Carassius auratus) died 4 weeks after purchase and was investigated by necropsy and histological examination. Routine formalin fixation of the goldfish was followed by histopathology. Formalin fixed spleen and kidney from the fish was further processed by embedding in epoxy resin and examined by transmission electron microscopy (EM). Severe, diffuse necrosis of haematopoietic tissue in the spleen, thymus and kidney and severe, diffuse hyperplasia in the gill epithelial cells were seen. In the spleen there was severe, diffuse necrosis of lymphocytes and many nuclei with marginated chromatin and intranuclear inclusions were scattered throughout the necrotic tissue. EM of affected tissues demonstrated intranuclear particles morphologically similar to herpesvirus. The presence of an agent similar to a herpesvirus in a goldfish with severe haematopoietic necrosis suggests that the herpesvirus responsible for haematopoietic necrosis in cyprinid species throughout the world has entered the goldfish population in Australia. 相似文献
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猪的心、肝、肺、脾、肾五脏器的发育早晚直接影响着其它部位的生长发育的快慢.运用异速生长方程分析了舍饲型合作猪内脏器官的生长发育规律.结果表明：肝脏和肺脏的增重迅速,肾脏和心脏次之,脾脏较慢;肺脏和肾脏的相对生长速度较快,其次为心脏和脾脏,肝脏较慢;各内脏器官占体重百分比为：肝脏＞肺脏＞肾脏＞心脏＞脾脏;异速生长方程拟合的内脏器官生长强度为：心脏（b=1.120）＞脾脏（b＝0.974）＞肺脏（b=0.973）＞肾脏（b=0.9520）＞肝脏（b=0.726）,心脏属于晚熟器官,脾脏、肺脏和肾脏属于中熟器官,肝脏属于早熟器官.为保护合作地方品种,进一步开发利用优良品种提供了数据参考. 相似文献
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分别以1日龄健康籽鹅肝脏、肾脏、心脏、肌肉和卵巢为研究对象,应用实时定量RT-PCR技术,探讨28SrRNA、18SrRNA、GAPDH、ACT、HPRT1、SDH和TUB等7个内参基因mRNA的表达水平。经过geNorm和NormFinder程序分析,结果显示7个内参基因稳定度由高到低依次为GAPDH=HRPT1〉28SrRNA〉TUB〉SDH〉ACT〉18SrRNA;基因表达的稳定值分别为0.215（GAPDH）,0.215（HRPT1）,0.339（28SrRNA）,0.471（TUB）,0.721（SDH）,0.888（ACT）,1.177（18SrRNA）;表明GAPDH和HRPT1这2个内参基因适合用于目的基因表达的校正。 相似文献
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应用光镜和电镜技术研究了人工感染雏番鸭细小病毒病死鸭的心、肝、脾、肺、肾、胰、法氏囊和大脑等8种器官的显微结构和发病鸭的心、肝、脾、肺、肾、胰等6种器官的超微结构变化。结果如下：1．显微结构变化主要表现为：各器官的血管扩张、充血，以肺脏尤为严重，并见少量淋巴单核细胞浸润，同时心、肝、肾、胰和大脑均呈现不同程度的变性；而免疫器官脾、法氏囊则主要表现为淋巴细胞数量减少。2．超微结构变化主要表现为：（1）各实质细胞内线粒体肿胀，嵴变少而或崩解，内质网减少，脂滴增多，部分细胞水肿坏死；（2）血管内皮均有不同程度的脂滴增多，水肿以至脱落；（3）吞噬能力加强，表现为细胞内次级溶酶体增多，吞噬细胞数量增加，其胞浆内含大量的吞噬体。 相似文献
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紫茎泽兰(Eupatorium adenophorum)在中国作为入侵物种,可造成食草动物中毒死亡,并给畜牧业带巨大的损失。为研究反刍动物采食紫茎泽兰后的中毒机理,本研究探讨了日粮中添加不同剂量的紫茎泽兰对萨能奶山羊(Saanen goat)血常规及肝、脾、肾的病理学影响。选择16只5~6月龄健康萨能奶山羊,随机分成对照组(日粮中未添加紫茎泽兰)和试验Ⅰ、Ⅱ、Ⅲ组(日粮配比中分别添加紫茎泽兰40%、60%和80%),每组4只。试验Ⅰ、Ⅱ、Ⅲ组分别饲喂含40%、60%和80%紫茎泽兰的混合日粮3个月,每两周检测一次血常规,试验末屠宰奶山羊,并观察其肝、肾、脾的病理学变化。结果表明,与对照组相比,各试验组血液中的白细胞(white blood cell,)和中性粒细胞(neutrophil,NEUT)均显著升高(P0.05),试验后期极显著高于对照组(P0.01);而各试验组血液中淋巴细胞(lymphocyte,LY)与血红蛋白(hemoglobin,HGB)均呈下降趋势,试验后期均极显著低于对照组(P0.01)。肝和脾肿大,肝组织出现淤血,肝细胞发生水泡变性及脂肪变性等,肾组织伴有出血、坏死、颗粒变性及水泡样变性的现象。因此,本研究结果说明紫茎泽兰对萨能奶山羊血液指标有显著影响,且长期摄入紫茎泽兰可引起动物肝、肾、脾等主要实质器官不同程度的损伤。 相似文献
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Hughes MS Ball NW Love DN Canfield PJ Wigney DI Dawson D Davis PE Malik R 《Journal of Feline Medicine and Surgery》1999,1(1):23-29
An 8-year-old FIV-positive Australian cat was presented with coughing, periocular alopecia, pyrexia and inappetence. Skin scrapings demonstrated Demodex cati mites. Antibiotics were administered and it was treated successfully for periocular demodectic mange, but the cat continued to exhibit respiratory signs and lose weight. Further investigation revealed an ascarid infection and active chronic inflammation of undetected cause affecting the lower airways. Repetitive treatment with pyrantel failed to eradicate the ascarid infection. The cat became cachectic and developed moist ulcerative dermatitis of the neck, severe non-regenerative anaemia, leucopenia and thrombocytopenia. Necropsy and histopathology revealed mycobacteriosis affecting skin, lungs, spleen, lymph nodes, liver and kidney. Attempted culture of frozen tissues at a mycobacteria reference laboratory was unsuccessful. Paraffin-embedded, formalin-fixed tissue was retrieved and examined using PCR to amplify part of the 16S rRNA gene. A diagnosis of disseminated Mycobacterium genavense infection was made based on the presence of acid fast bacteria in many tissues and partial sequence of the 16S rRNA gene. Although M genavense has been identified previously as a cause of disseminated disease in AIDS patients, this is the first report of infection in a cat. It was suspected that the demodecosis, recurrent ascarid infections and disseminated M genavense infection resulted from an immune deficiency syndrome consequent to longstanding FIV infection. 相似文献
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Epidemiology, pathology, and immunohistochemistry were investigated in layer hens affected with H5N1 highly pathogenic avian influenza, which occurred for the first time in 79 years in Japan. The farm, which had a total of 34,640 chickens, experienced up to 43.3% mortality before the chickens were depopulated. Clinically, the affected chickens exhibited mortality without apparent clinical signs. Histologically, hepatocytic necrosis; necrosis of ellipsoids and follicles with fibrin in the spleen; necrosis with glial nodules in the brain stem, cerebrum, and cerebellum; necrosis of acinar cells in the pancreas; and necrosis of lymphoid tissues in intestinal lamina propria were seen. Occasionally, mild bronchiolitis, degeneration of smooth muscle fibers in the cecum, and mild tubulonephrosis were noted. Immunohistochemically, influenza virus antigens were detected often in the liver and spleen, heart, intestine, gizzard, proventriculus, and oviduct. In addition, antigens were seen also in the brain, kidney, pancreas, and ovary, but seldom in the lung and trachea. Virus antigen was mainly detected in the capillary endothelium and parenchymal cells. This suggests that virus excretion from the respiratory tract was not as prevalent as that from the digestive tract in the present cases. 相似文献
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腹泻犊牛大肠埃希菌的分离鉴定及药敏试验 总被引：1，自引：0，他引：1
对陕西省某奶牛场一起犊牛腹泻病例进行诊断和治疗。无菌采集腹泻犊牛肝脏和脾脏组织进行微生物学检测、小鼠致病性试验和药敏试验。结果显示,被检测病料中,牛病毒性腹泻病毒(BVDV)、牛腺病毒7型(BAdV-7)、牛冠状病毒(BCoV)、牛轮状病毒(BRV)检测结果均为阴性;分别从脾脏和肝脏组织分离培养到2株细菌,其培养特性和生化试验结果与大肠埃希菌高度相似,16S rRNA基因测序结果与大肠埃希菌的同源性为100%;动物致病性试验显示,分离菌能致试验小鼠死亡;药敏试验结果显示,该菌对左氧氟沙星、庆大霉素高度敏感,对头孢呋辛、恩诺沙星不敏感;临床选择敏感药物左氧氟沙星进行治疗,犊牛腹泻很快得到控制。 相似文献
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Seven 19-day-old broiler chickens affected with hydropericardium syndrome (HPS) with pancreatic necrosis and gizzard erosions were investigated pathologically and virologically. Mortality increased after 13 days of age in a flock on a broiler farm. The mortality rate of the flock reached 10% by 19 days of age. Macroscopically, the chickens had hydropericardium (the characteristic gross change of HPS), pinpoint white foci in the pancreas, and ventricular erosions. Histologically, the chickens had multifocal hepatic necrosis with intranuclear inclusions in hepatocytes, a marked increase of macrophages in the spleen and lung, mild epicardial edema, multifocal necrosis of pancreatic acinar cells with intranuclear inclusions, focal necrosis of the ventricular koilin layer, and degeneration of the ventricular glandular epithelium with intranuclear inclusions. Immunohistochemically, intranuclear inclusions in the liver, pancreas, and ventriculi were stained positively against group I avian adenovirus (GIAAV) antigens. Ultrastructurally, 67-nm diameter viral particles were present in intranuclear inclusions. Virologically, serotype 4 of GIAAV was isolated from the liver, heart, and kidney of affected chickens. The pathologic changes of the present cases differ from previous cases of HPS; therefore, the present strain of GIAAV may have different pathogenicity for chickens than the previous virus strain of HPS. 相似文献
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Bowater RO Norton J Johnson S Hill B O'Donoghue P Prior H 《Australian veterinary journal》2003,81(10):627-632
OBJECTIVE: To describe the clinical signs, gross pathology, serology, bacteriology, histopathology, electron microscopy and immunohistochemistry findings associated with toxoplasmosis in four Indo-Pacific humpbacked dolphins (Sousa chinensis) that stranded in Queensland in 2000 and 2001. DESIGN: Clinical assessment, gross necropsy, and laboratory examinations. PROCEDURE: Necropsies were performed on four S. chinensis to determine cause of death. Laboratory tests including serology, bacteriology, histopathology and transmission electron microscopy were done on the four dolphins. Immunohistochemistry was done on the brain, heart, liver, lung, spleen and adrenal gland from various dolphins to detect Toxoplasma gondii antigens. RESULTS: Necropsies showed all of four S. chinensis that stranded in Queensland in 2000 and 2001 had evidence of predatory shark attack and three were extremely emaciated. Histopathological examinations showed all four dolphins had toxoplasmosis with tissue cysts resembling T. gondii in the brain. Tachyzoite stages of T. gondii were detected in the lungs, heart, liver, spleen and adrenal gland, variously of all four dolphins. Electron microscopy studies and immunohistochemistry confirmed the tissues cysts were those of T. gondii. All four dolphins also had intercurrent disease including pneumonia, three had peritonitis and one had pancreatitis. CONCLUSION: Four S. chinensis necropsied in Queensland in 2000 and 2001 were found to be infected with toxoplasmosis. It is uncertain how these dolphins became infected and further studies are needed to determine how S. chinensis acquire toxoplasmosis. All four dolphins stranded after periods of heavy rainfall, and coastal freshwater runoff may be a risk factor for T. gondii infection in S. chinensis. This disease should be of concern to wildlife managers since S. chinensis is a rare species and its numbers appear to be declining. 相似文献
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对患溶组织内阿米巴原虫病而死亡的凹甲陆龟进行了系统的病理组织学观察，该病的病变主要发生在消化道、肝、心脏、肾、脾等部位，尤以胃肠道和肝病变最为明显，表现为卡他性一坏死性胃肠炎和坏死性一肉芽肿性肝炎，胃肠黏膜出血、水肿、上皮细胞变性、坏死脱落，形成溃疡灶；肝细胞水泡变性，细胞核裂解，肝小叶内可见大小不等的结节性坏死灶，并伴有明显的炎症反应。 相似文献
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Jiao J Yuan T Zhou Y Xie W Zhao Y Zhao J Ouyang H Pang D 《Journal of animal science》2011,89(10):3099-3106
Myostatin is expressed in skeletal muscle tissue where it functions to suppress myoblast proliferation and myofiber hypertrophy. Recently, myostatin was detected in the tendon, mammary gland, and adipose tissue of mice. We sought to determine whether myostatin is expressed in the liver, spleen, lung, and kidney of pigs. Real-time PCR and Western blots demonstrated that myostatin, follistatin, decorin, and activin receptor IIB (ActRIIB) mRNA and proteins were expressed in skeletal muscle, heart muscle, and adipose tissue, and also in liver, spleen, lung, kidney, and cultured fibroblasts. The relative abundance of myostatin was closely related to follistatin and decorin in porcine tissues. Immunohistochemical analysis further demonstrated the presence of myostatin, follistatin, and decorin in the skeletal muscle, adipose tissue, heart muscle, liver, spleen, lung, and kidney of pigs. These results suggest that myostatin could be associated with certain functions of the internal organs, such as energy metabolism or fibrosis. We conclude that myostatin is a factor broadly expressed in the internal organs and muscle tissues of pigs. 相似文献