共查询到19条相似文献，搜索用时 250 毫秒
1.
应用免疫组化技术并结合图像分析软件研究了胃泌素、β-内啡肽、胰高血糖素、5-羟色胺、生长抑素在鸭腔上囊中的表达特征。结果显示：鸭腔上囊小结相关上皮细胞呈胃泌素强阳性反应;滤泡间粘膜上皮呈β-内啡肽中强度阳性反应;淋巴滤泡皮质部有少量的β-内啡肽、胃泌素、5-羟色胺阳性细胞,呈中、强度免疫反应;淋巴滤泡髓质有胃泌素强阳性细胞;生长抑素和胰高血糖素均呈阴性反应。结果说明,鸭腔上囊中存在β-内啡肽、胃泌素、5-羟色胺弥散神经内分泌细胞,它们在淋巴滤泡皮质部的表达特征有利于其通过内分泌和旁分泌方式调节B细胞的发育。 相似文献
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应用免疫组化技术并结合图像分析软件研究了胃泌素、β-内啡肽、胰高血糖素、5-羟色胺、生长押素在鸭腔上囊中的表达特征.结果显示鸭腔上囊小结相关上皮细胞呈胃泌素强阳性反应;滤泡间粘膜上皮呈β-内啡肽中强度阳性反应;淋巴滤泡皮质部有少量的β-内啡肽、胃泌素、5-羟色胺阳性细胞,呈中、强度免疫反应;淋巴滤泡髓质有胃泌素强阳性细胞;生长抑素和胰高血糖素均呈阴性反应.结果说明,鸭腔上囊中存在β-内啡肤、胃泌素、5-羟色胺弥散神经内分泌细胞,它们在淋巴滤泡皮质部的表达特征有利于其通过内分泌和旁分泌方式调节B细胞的发育. 相似文献
3.
旨在研究前列腺素家族中生殖领域鲜有报道的PGD2成员对单一环境下黄体细胞内分泌功能及其凋亡相关基因表达的影响，并解析其在黄体退化中的作用机理，为全面探析前列腺素家族成员的生物学作用提供新的理论依据。采集空怀母山羊黄体中期的卵巢组织，通过胶原酶II消化和胰酶差速离心法分离纯化以获得山羊黄体细胞。采用DMEM/F12进行离体培养，观察不同离体培养时间的黄体细胞生长状态；采用免疫组化法和细胞形态特征鉴定黄体细胞，将PGD2设置三个不同梯度确定其对黄体细胞作用效果的剂量依赖性关系。最后，通过PGD2最佳依赖性剂量处理黄体细胞，利用ELISA法检测黄体细胞的内分泌功能变化，流式细胞术测定黄体细胞的凋亡率及qRT-PCR/Western blot法检测凋亡相关基因mRNA/蛋白表达水平。结果显示，经突触素(synaptophysin, SYP)特异性表达蛋白鉴定，成功分离并获得了山羊原代黄体细胞。细胞形态实时观察和ELISA检测结果显示，离体培养5 d时黄体细胞胞质饱满、外形紧凑、形态清晰可见，并且黄体细胞的内分泌P4 相似文献
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5.
运用免疫组化ABC法妊娠26～120d的奶山羊黄体中催产素免疫反应细胞的分布进行了观察。结果表明，奶山羊妊娠黄体中存在催产素(Oxytocin,OT）免疫反应阳性细胞。阳性细胞在形态上以卵圆形、圆形、棱形为主，还有一些具有明显的突起。根据阳性细胞胞质内反应颗粒着色的深浅，可把OT阳性细胞分为强阳性、中等阳性和弱阳性3种。在妊娠26～30d，阳性细胞数量最多，强阳性细胞主要分布于黄体的周边，中等阳性及弱阳性细胞则均匀分布于整个黄体组织中，妊娠31～60d，阳性细胞数量明显下降，弥散于整个黄体组织中；妊娠61～120d，阳性细胞的数量及逐渐增多，以中等阳性和弱阳性细胞为主，而强阳性细胞数量较少。连续切片HE染色的对照观察显示妊娠黄体中大、小黄体细胞均可出现OT免疫阳性反应。 相似文献
6.
应用免疫组化技术并结合图像分析软件研究了β-内啡肽、胃泌素、胰高血糖素、5-羟色胺、血管活性肠肽、神经肽Y、生长抑素细胞在鸭胸腺中的表达特征。结果显示：7种神经内分泌细胞在胸腺中呈强阳性表达;β-内啡肽阳性细胞在皮质部较多,而胃泌素、胰高血糖素、5-羟色胺、血管活性肠肽、神经肽Y、生长抑素阳性细胞在髓质和皮髓质交界区的数量高于皮质部;除胰高血糖素外,其余6种神经内分泌激素在胸腺小体中呈不同程度的阳性反应。本试验结果说明,鸭胸腺不仅是中枢免疫器官,而且具有重要的神经内分泌功能;β-内啡肽阳性细胞在T淋巴细胞的发育中发挥重要作用;胃泌素、胰高血糖素、5-羟色胺、生长抑素、血管活性肠肽、神经肽Y阳性细胞在髓质部和皮髓质交界区的表达有利于其通过内分泌、自分泌或旁分泌方式调节T细胞的发育。同时,也探讨了胸腺小体的功能。 相似文献
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8.
为探讨催产素(OT)是否可以影响雌性山羊颈胸神经节的活动.采用免疫组化SP法观察催产素受体在雌性山
羊颈胸神经节的分布特点.催产素受体在颈胸神经节分布广泛,节内的神经细胞、卫星细胞和过路纤维均有OTR
免疫阳性产物分布;OTR主要在神经细胞中表达,相对表达量与其他非神经结构相比差异性显著(P＜0.05);在神经
细胞中,OTR免疫阳性产物主要存在于胞膜、胞质、核仁,核膜不着色.雌性山羊颈胸神经节神经元对OT具有反应
性,提示OT可能通过影响颈胸神经节神经元的活动,从而经其发出的交感节后神经这一途径调节其所支配的靶器
官如心血管、汗腺、呼吸等的生理活动. 相似文献
9.
发情周期中奶山羊下丘脑-垂体-卵巢轴催产素的免疫组化定位 总被引：9，自引：0，他引：9
用免疫组化ABC法,对发情周期中奶山羊下丘脑-垂体-卵巢轴催产素(OT)分布进行了观察研究.结果表明,下丘脑中分泌OT的神经元主要分布在室旁核和视上核,在穹窿周核、腹内侧核、腹外侧核、交叉上核、背内侧核、乳头体、下丘脑外侧区、下丘脑前核等核团也有一定数量的阳性神经元;阳性神经纤维仅见于室旁核、下丘脑前核、视上核等少数核团,在正中隆起和第3脑室室周可见到一定数量的阳性神经纤维.在垂体前叶未见到OT免疫反应阳性产物,自垂体柄和正中隆起的一侧可见到平行排列的OT阳性神经纤维断续地延伸至神经部.卵巢的卵泡及间质未见OT免疫阳性反应,,在黄体组织中存在数量较多的免疫反应阳性细胞,阳性细胞主要呈圆形、卵圆形,小梁两侧及黄体中央近腔区域的阳性细胞呈长梭形,有相当数量的阳性细胞具有突起.连续切片HE染色对照观察显示,黄体中OT主要由大黄体细胞产生,但小黄体细胞也存在OT免疫阳性反应. 相似文献
10.
《动物医学进展》2016,(7)
为了探讨5-羟色胺(5-HT)与山羊黄体细胞的生长是否存在密切的关联性,揭示其在黄体发育中的生物学作用,采用胶原酶Ⅱ消化法获得原代山羊黄体细胞并用催产素(OT)染色对细胞进行鉴定,四甲基偶氮唑蓝(MTT)比色法测定不同浓度5-HT对山羊黄体细胞的影响,免疫组织化学染色法(SP法)检测山羊黄体细胞中增殖细胞核抗原(PCNA)蛋白的表达。MTT法结果显示,5-HT对山羊黄体细胞的增殖起到促进作用,促增殖的浓度范围在10~(-8) mol/L~10~(-4) mol/L,浓度越高,促增殖作用越明显。免疫组织化学染色法证实,5-HT能够使山羊黄体细胞中PCNA蛋白的表达增多。结果提示,5-HT可能是通过促进PCNA蛋白的表达来实现对山羊黄体细胞的促增殖作用。5-HT在黄体发生、发展过程中发挥的重要作用及相关分子机制的初步揭示都为生殖内分泌系统的研究奠定了理论基础。 相似文献
11.
The gross, histomorphologic, cytochemical, and immunocytochemical findings in 16 dogs with medullary thyroid carcinoma were evaluated. Grossly, the neoplasms were encapsulated, firm, lobulated, and grey-white to tan. The typical histologic pattern was groups or sheets of round to polygonal cells with fibrovascular stroma, which was thickened and hyalinized in places. Variants of clear cell (two dogs), giant cell (one dog), and oxyphil cell (one dog) types were also seen. In all 16 dogs, Grimelius-stained sections of the neoplasms revealed intracytoplasmic silver granules; ten tumors contained amyloid and four contained mucin. Immunohistochemically, the neoplasms reacted to AE1/AE3 (n = 13), S-100 protein (n = 5), neuron specific enolase (n = 14), synaptophysin (n = 11), calcitonin (n = 16), somatostatin (n = 4), gastrin (n = 7), and serotonin (n = 6). Only one neoplasm was positive for vimentin. None of the neoplasms reacted to antibodies for neurofilaments, thyroglobulin, insulin, glucagon, or adrenocorticotrophic hormone. Eleven neoplasms contained multiple (two to four) peptides, in various combinations. It was concluded that in dogs, gross and histologic features can be used to distinguish medullary thyroid carcinoma from other thyroid malignancies. Cytochemical and immunocytochemical studies with neuron specific enolase, synaptophysin, and calcitonin can be used to establish the diagnosis of medullary thyroid carcinoma in dogs. 相似文献
12.
Egerbacher M Helmreich M Probst A König H Böck P 《Anatomia, histologia, embryologia》2005,34(2):112-116
Digital cushions were studied in horses with particular reference to vascularization, tissue constituents and matrix components. The cushions mainly resembled a network of coarse collagen bundles. The areas inbetween the bundles were replenished with loosely woven interstitial connective tissue, myxoid tissue, and fibrocartilage. Expected masses of fat lobules were missing: only solitary adipocytes or small groups of adipocytes were seen. Vascular supply to the cushions was remarkably poor. The mucinous myxoid matrix largely consisted of hyaluronan with little sulphated glycosaminoglycans. Myxoid cells were stellate or ramified in shape and showed a tendency to store glycogen and lipid droplets. Myxoid cells reacted for vimentin and stained for S-100 protein. Moreover, myxoid cells often reacted for neuron specific enolase and glial fibrillary acidic protein. Myxoid tissue continuously transformed into loosely organized interstitial connective tissue with fibroblasts, which remained unreactive when tested for neuroectodermal markers. Myxoid tissue also was not clearly demarcated against irregularly interspersed islets of fibrocartilage or hyaline cartilage. Chondrocytes did not stain for neuron specific enolase but reactivity for S-100 protein and glial fibrillary acidic protein was noted in peripheral regions of fibrocartilage. Single or grouped unilocular fat cells were rarely placed into myxoid areas. Unilocular fat cells stained for vimentin, S-100 protein, and occasionally for glial fibrillary acidic protein but not for neuron specific enolase. Continuous transformation of myxoid tissue into cartilage together with corresponding reactivity for neuroectodermal marker proteins of myxoid cells and peripherally located chondrocytes suggest close relationship between myxoid cells and chondrocytes. The same criteria indicate relationship between myxoid cells and adipocytes. Coarse connective tissue, myxoid tissue, fibrous cartilage, and fat cells are functionally combined to absorb mechanical shock in the horse digital cushions. 相似文献
13.
HH Ortega NR Salvetti C Baravalle JA Lorente GA Mira 《Reproduction in domestic animals》2006,41(3):204-209
The objectives of this work were to determine the changes in the expression of neuroendocrine markers in Leydig cell by oestradiol treatment, and to determine whether testosterone is able to recover partially the effects of hormonal suppression induced by oestradiol. Adult male rats were injected daily with either 50 microg of oestradiol or oestradiol plus testosterone propionate (25 mg every 3 days) for 15 days. The animals were sacrificed and testicles were dissected and processed by routine histological protocols. FSH and LH serum levels were determined by radioimmunoassay. The visualization of antigens was achieved by the streptavidin-peroxidase immunohistochemical method. Antibodies against chromogranin A (CrA), S-100 protein (S-100), P substance (PS), synaptofisin (SYN), neurofilament protein (NF), gliofibrillary acidic protein (GFAP) and neuron specific enolase (NSE) were used. The mean LH and FSH serum concentrations were consistently suppressed with hormonal treatments. Intermediate filaments (NF and GFAP) showed no difference in their expression. The expression of S-100, NSE and SYN was significantly lower in both hormone-treated groups. In oestradiol-treated rats, the immunoreactivity of CrA and SP decreased significantly but was restored after testosterone supplementation. Although the nature and functions of many of these substances in Leydig cells remain unknown, these results are consistent with the hypothesis that the expression of some neuroendocrine markers is hormonally controlled. 相似文献
14.
C Geyer A Hafner S Pfleghaar W Hermanns 《Zentralblatt für Veterin?rmedizin. Reihe B. Journal of veterinary medicine. Series B》1992,39(7):485-494
Six canine, one feline and one equine granular cell tumours (GCTs) were investigated electron microscopically and immunohistochemically. The tumours were tested for reactivity with monoclonal antibodies against vimentin and desmin and with polyclonal antibodies against cytokeratin, S-100 protein, glial fibrillary acidic protein (GFAP) and neuron specific enolase (NSE). All GCTs were characterized by their PAS positive cytoplasmic granules in light microscopy, which in electron microscopy appeared as lysosome-like granules. In each case two canine GCTs were stained by the antibody against cytokeratin, vimentin and S-100 protein. Cells of the equine GCT showed reactivity with the antiserum against S-100 protein. In the feline GCT no reactivity with any of the antibodies tested was observed. These differences of the immunohistochemical reactions of GCTs suggest a nonuniform histogenesis of GCTs in domestic animals. The reactivity of the tumour cells with the antiserum against NSE is discussed. 相似文献
15.
Rigas JD Smith TJ Gorman ME Valentine BA Simpson JM Seguin B 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2012,41(1):141-146
An 8-year-old male neutered Pomeranian dog was presented to the Veterinary Teaching Hospital at Oregon State University for surgical treatment of hydronephrosis of the left kidney and a left cranial abdominal mass. A primary ureteral mass was found during exploratory surgery, and the mass was resected and ureteral anastomosis was performed. Cytologic evaluation of the mass revealed 3 distinct cell populations, including a large number of multinucleated giant cells, a moderate number of thin spindle-shaped cells, and cohesive clusters of transitional epithelial cells. The cytologic diagnosis was giant cell sarcoma. The diagnosis was confirmed by histologic examination, and immunohistochemical staining was performed. The spindle-shaped cells and multinucleated giant cells were both immunoreactive for vimentin and spindle-shaped cells for S-100. Tumor cells did not express wide-spectrum cytokeratin, broad-spectrum muscle actin, smooth muscle actin, sarcomeric actin, desmin, BLA36, Mac 387, synaptophysin, neuron-specific enolase, glial fibrillary acid protein, or von Willebrand factor. These findings are most consistent with an anaplastic sarcoma with giant cells. This is the first case report of a primary ureteral giant cell sarcoma in a dog. 相似文献
16.
蓝狐消化道5-羟色胺和生长抑素免疫组化定位 总被引：2，自引：0，他引：2
采用免疫组织化学SP法.研究蓝狐消化道内5-羟色胺和生长抑素2种免疫阳性细胞的形态结构与分布密度.结果表明,消化道中这2种免疫阳性细胞形态多呈圆形、椭圆形或锥体形,主要集中分布在胃腺上皮、肠上皮及肠腺上皮细胞之间.5-HT免疫阳性细胞数量以结肠最多,直肠和空肠次之,胃底腺区、十二指肠、回肠和幽门腺区较少,食管、贲门腺区和盲肠中未见:SS免疫阳性细胞大量出现于幽门腺区,食管、责门腺区和盲肠中未见;根据其形态推测,蓝狐消化道这2种免疫阳性细胞有内、外2种分泌功能. 相似文献
17.
Sawamoto O Yamate J Kuwamura M Hagiwara R Kurisu K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1999,61(12):1335-1338
Peripheral nerve sheath tumor was found in a 7-year-old male mongrel dog. The tumors were located in the right cheek subcutis and oral submucosa. Histologically, neoplastic cells were arranged in streaming bundles, occasionally interlacing bundles or whorls of elongated and spindle cells. Cellular atypia was poor and mitotic figures were rarely observed. Ultrastructurally, neoplastic cells had basement membrane, typical of Schwann cells. One bundle of normal peripheral nerve fibers and some myelinated axons were seen within the tumor tissues. Immunohistochemically, neoplastic cells reacted to vimentin, glial fibrillary acidic protein, S-100 protein and neuron specific enolase. In addition to the above immunoreactions, the included nerve fibers were positive for myelin basic protein and neurofilament protein. This paper also discusses immunohistochemical findings on differential diagnosis in comparison with those of canine hemangiopericytomas reported hitherto. 相似文献
18.
Yamate J Izawa T Ogata K Kobayashi O Okajima R Kuwamura M Kotani T Aoki M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(5):495-498
An 11-year-old thoroughbred gelding was euthanatized because of right nasal cavity tumor. The tumor consisted of round to oval cells with a scanty cytoplasm and hyperchromatic nuclei. Homer-Wright rosettes and pseudorosettes, as well as microcysts were seen. Neoplastic cells were immunoreactive to vimentin, S-100 protein, and neuron-specific enolase, glial fibrillary acidic protein and microtube-associated protein in varying degrees, indicating neurogenic nature. Based on these findings, this tumor was diagnosed as an olfactory neuroblastoma. Since this type is an uncommon tumor showing histological variety, the nature is discussed. 相似文献
19.
Aresu L Tursi M Iussich S Guarda F Valenza F 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(11):1229-1233
To define the characteristics of malignancy we performed routine histology and an immunohistochemical study on seventeen aortic body tumors in dogs. We essayed tumors using a panel of immunohistochemical markers: neuron specific enolase (NSE), chromogranin A (CrA) and S-100. Among 17 cases, the neoplastic cells were positive for NSE (17 cases, 100%), S-100 (9 cases, 53%), and CrA (8 cases, 47%), respectively. The sustentacular cells density and chief cell staining intensity were both inversely related to tumor grade. The most relevant data was consistent with a negative staining of S-100 correlated with absence or decreased number of sustentacular cells in tumors grade III. This report indicates that the immunohistochemical panel has utility for the diagnosis of chemodectoma and the negative staining to CrA and S-100 markers in tumors grade III expresses an indication of malignant behaviour of the tumor. 相似文献