共查询到20条相似文献，搜索用时 31 毫秒
1.
La Rosa G Muscillo M Di Grazia A Fontana S Iaconelli M Tollis M 《Journal of veterinary medicine. B, Infectious diseases and veterinary public health》2006,53(6):257-265
Porcine enteroviruses (PEVs) and teschoviruses (PTVs) are described as causative agents of neurological disorders, fertility disorders and dermal lesions of swine. Difficulties in the serological detection of these viruses may lead to a significant underestimation of infections with clinical symptoms. With the recent availability of genome sequence data for all the serotypes, molecular diagnosis is a possibility. The present study describes a new approach to molecular 'serotyping' of PTVs and PEV-B viruses, involving the amplification and sequencing of a genomic fragment of the VP1 coding region. A molecular characterization of Italian entero-teschovirus isolates was performed using a set of previously published and newly designed polymerase chain reaction primers. A total of 33 porcine isolates and 10 reference strains were analysed. Porcine enterovirus-B samples were first diagnosed as positive for enterovirus by amplification of the 5'-non-translated region. Samples were then typed by amplification and sequencing of a portion of the VP1 coding region. Porcine enterovirus-A and PTVs were detected by a published assay in the 5'-NC region that allows them to be differentiated according to the size of amplification product, using the same set of primers. For serotype characterization of PTV, we evaluated four different regions: the N terminus of the capsid protein VP2, the region encoding for RNA-dependent RNA polymerase, and the capsid VP1 and VP4 regions. The newly designed primers in the VP1 region was proved to be broad in range and suitable for serotype assessment and therefore constitute a useful diagnostic tool for molecular diagnosis of porcine teschovirus/enterovirus strains and for the study of molecular epidemiology and evolution of these viruses. 相似文献
2.
3.
CHEN Ru-jing XIU Jin-sheng CHEN Xiao-luo WU Xue-min CHE Yong-liang WANG Chen-yan YAN Shan WANG Long-bai ZHOU Lun-jiang 《中国畜牧兽医》2015,42(9):2303-2307
In this study, specific primers were designed according to VP0 gene of porcine kobuvirus (PKV), and the full-length coding region of VP0 gene was amplified by RT-PCR method and sequenced, then bioinformatics analysis were conducted to investigate the structure and function of the porcine kubovirus VP0 gene.The results showed that the porcine kobuvirus VP0 gene was 1 098 bp in length, coding an open reading frame (ORF) with 366 amino acids.The isoelectric point, molecular weight and instability index of porcine kubovirus VP0 were 6.71, 38.489 ku, 34.65, respectively.The maximum and minimum hydrophobicity were 2.622 and —2.122, respectively.Compared with the porcine kubovirus VP0 genes published previously in GenBank, the sequenced gene shared the highest homology with HNXX-4 strain, which was 89.1%;And shared the lowest homology with S-1-HUN strain, which was 81.1%.The porcine kubovirus VP0 gene shared two different phylogenetic genotype branches, and the Chinese porcine kubovirus isolates distributed in the two phylogenetic genotype branches. 相似文献
4.
本研究根据猪嵴病毒(porcine kobuvirus,PKV)VP0基因序列特征设计特异性扩增引物,采用RT-PCR方法扩增猪嵴病毒VP0基因全长编码区。将特异性扩增目的片段克隆后进行序列测定,将结果进行拼接后获得猪嵴病毒VP0基因全长并进行相关生物信息学分析。所扩增的目的片段编码有完整的VP0基因开放阅读框,全长为1 098 bp,编码有366个氨基酸,理论等电点为6.71,理论分子质量为38.489 ku,不稳定系数为34.65,最大疏水指数为2.622,最小疏水指数为-2.122。将获得的VP0基因和GenBank中的猪嵴病毒代表株VP0基因序列进行核苷酸同源性比对和遗传进化分析,其与HNXX-4核苷酸同源性最高,为89.1%,与S-1-HUN核苷酸同源性最低,为81.1%。从遗传进化上看,猪嵴病毒VP0基因在遗传进化上呈两个独立的基因亚群,猪嵴病毒中国分离株在两个遗传基因亚群上均有分布。 相似文献
5.
6.
7.
Biswas S Sanyal A Hemadri D Tosh C Mohapatra JK Manoj Kumar R Bandyopadhyay SK 《Veterinary microbiology》2006,116(1-3):187-193
A total of 18 foot-and-mouth disease virus (FMDV) serotype Asia1 field isolates belonging to two different lineages (including the divergent group) as delineated earlier in VP1-based phylogeny were sequenced in the non-structural 3A and 3C protein-coding regions. The phylogenetic trees representing the regions coding for the non-structural proteins were very similar to that of the structural VP1 protein-coding region. Phylogenetic comparison at 3C region revealed clustering of Asia1 viruses with the isolates of serotypes O, A and C in the previously identified clade. Comparison of amino acid sequences identified lineage-specific signature residues in both the non-structural proteins. Overall analysis of the amino acid substitutions revealed that the 3A coding region was more prone to amino acid alterations than 3C region. 相似文献
8.
THE CHARACTERISATION AND PATHOGENICITY OF PORCINE ENTEROVIRUSES ISOLATED IN VICTORIA 总被引：1，自引：0，他引：1
SUMMARY Approximately 23 viruses were isolated from healthy pigs, pigs with encephalitis, and in cases of reproductive failure. Five viruses were identified as enteroviruses and a total of 10 isolates were shown to cross-react serologically to varying degrees. Twenty viruses were neutralised by a reference antiserum of serotype 8 porcine enterovirus. Intracerebral inoculation of colostrum-deprived piglets with 2 of the characterised viruses caused lesions of encephalomyelitis which were not induced by oral infection. Intrafoetal inoculation of 2 sows with one characterised faecal isolate caused foetal death and abortion, but no adverse effects followed oral dosage. 相似文献
9.
10.
11.
The surface exposed capsid proteins, VP1, VP2 and VP3, of foot-and-mouth disease virus (FMDV) determine its antigenicity and the ability of the virus to interact with host-cell receptors. Hence, modification of these structural proteins may alter the properties of the virus.In the present study we compared the pathogenicity of different FMDVs in young pigs. In total 32 pigs, 7-weeks-old, were exposed to virus, either by direct inoculation or through contact with inoculated pigs, using cell culture adapted (O1K B64), chimeric (O1K/A-TUR and O1K/O-UKG) or field strain (O-UKG/34/2001) viruses. The O1K B64 virus and the two chimeric viruses are identical to each other except for the capsid coding region.Animals exposed to O1K B64 did not exhibit signs of disease, while pigs exposed to each of the other viruses showed typical clinical signs of foot-and-mouth disease (FMD). All pigs infected with the O1K/O-UKG chimera or the field strain (O-UKG/34/2001) developed fulminant disease. Furthermore, 3 of 4 in-contact pigs exposed to the O1K/O-UKG virus died in the acute phase of infection, likely from myocardial infection. However, in the group exposed to the O1K/A-TUR chimeric virus, only 1 pig showed symptoms of disease within the time frame of the experiment (10 days). All pigs that developed clinical disease showed a high level of viral RNA in serum and infected pigs that survived the acute phase of infection developed a serotype specific antibody response. It is concluded that the capsid coding sequences are determinants of FMDV pathogenicity in pigs. 相似文献
12.
A L Green N M DuTeau M W Miller J M Triantis M D Salman 《American journal of veterinary research》1999,60(5):583-588
OBJECTIVE: To evaluate 2 polymerase chain reaction (PCR)-based methods for differentiating cytotoxic and noncytotoxic Pasteurella trehalosi from Rocky Mountain bighorn sheep (Ovis canadensis canadensis). SAMPLE POPULATION: 23 isolates of P. trehalosi from bighorn sheep in Colorado, including 18 from free-ranging herds and 5 from a captive herd. PROCEDURE: Using a sequence of the leukotoxin gene region of P. haemolytica serotype 1, 7 PCR primers were designed. A PCR amplification was performed on a sample of bacterial cell suspensions from pure cultures of P. trehalosi with known in vitro cytotoxic effects. The 2 most promising primer pairs were used in a study of 23 P. trehalosi isolates. Results were analyzed for association with cytotoxicity and 3 distinct ribotypes (Eco, Aco, and Bco). RESULTS: Significant associations were observed between in vitro cytotoxicity and PCR results for coding region, between ribotype Eco classification and PCR results for coding region, and between ribotype Eco classification and PCR results for promoter region. There was a negative association between ribotype Aco classification and PCR results for coding and promoter regions. CONCLUSIONS AND CLINICAL RELEVANCE: The PCR for the leukotoxin A coding region may be useful in differentiating cytotoxic from noncytotoxic P. trehalosi isolates recovered from bighorn sheep. It may be useful for studying epidemiologic features of pasteurellosis in bighorn sheep and for designing vaccines to protect wild sheep against pneumonia caused by P. trehalosi and P. haemolytica. 相似文献
13.
In 1999, 10 sporadic outbreaks of cattle foot-and-mouth disease (FMD) occurred in Taiwan. By the time, infection was limited to the Chinese yellow cattle (a native species of beef cattle in Mainland China), which did not develop vesicular lesions under field conditions. Five viruses isolates obtained from individual farms were confirmed to be the serotype O FMD virus (O/Taiwan/1999). During January-February 2000, however, this virus has spread to dairy cattle and goat herds, causing severe mortality in goat kids and vesicular lesions in dairy cattle. Partial nucleotide sequence of the capsid coding gene 1D (VP1) was determined for the virus isolates obtained in this study. Phylogenetic analysis of the VP1 sequences indicated that the O/Taiwan/1999 viruses shared 95-97% similarities to the virus strains isolated from the Middle East and India. The species susceptibility of the O/Taiwan/1999 virus was experimentally studied in several species of susceptible animals, showing that the virus did cause generalized lesions in dairy cattle and pigs, however, it would not cause vesicular lesions on the Chinese yellow cattle and the adult goats. These studies suggested that the O/Taiwan/1999 virus was a novel FMD virus of Taiwan and it presented various levels of susceptibility in cattle species. 相似文献
14.
北京地区某猪场猪群疑似感染猪捷申病毒(porcine teschovirus,PTV),为了分离与鉴定该病毒,从采集的脑组织样品中分离出病毒,并从病毒中提取总RNA,根据已报道的引物序列,经RT-PCR扩增得到部分编码主要结构蛋白的VP1基因和高度保守的5'-UTR区基因序列,将这些基因克隆到pEASY-Blunt载体中,经序列测定,与GenBank上的毒株序列进行比对。试验结果表明,分离的毒株与猪捷申病毒同源性为95%~100%。本试验成功分离获得一株猪捷申病毒北京地方流行毒株,命名为 10BJ02株。 相似文献
15.
Ray Pradeep K. Desingu P. A. Anoopraj R. Singh R. K Saikumar G. 《Tropical animal health and production》2020,52(3):1161-1166
Tropical Animal Health and Production - Porcine teschovirus (PTV) previously classified as porcine enteroviruses in the family Picornaviridae are associated with a wide range of illnesses in swine... 相似文献
16.
A complement fixation test in microtitre plates for the differentiation of porcine enterovirus serotypes is described, employing guinea pig antisera prepared using inactivated purified viruses. Eleven porcine enterovirus serotypes and swine vesicular disease virus were compared and clearly distinguished from each other. In addition, 71 porcine enterovirus strains and isolates were tested and each was identified as belonging to one of the 11 serotypes. 相似文献
17.
Nucleic acid and structural proteins of infectious bursal disease virus isolates belonging to serotypes I and II 总被引：1，自引：0，他引：1
The nucleic acid and structural proteins of infectious bursal disease virus serotype I (six isolates) were compared with those of serotype II (two isolates). Five of the serotype I isolates originated from chickens, whereas both serotype II isolates were from turkeys. The growth curves of representative isolates of both serotypes were similar, but the latent period and virus yield were different. The seven isolates tested had two segments of double-stranded genomic RNA. The RNA migration patterns of viruses belonging to each serotype were similar, but differences were noticed between the two serotypes. There were differences in the molecular weights of viral proteins (VP) 3 and 4 from the two serotypes, and serotype II isolates lacked VP-2. 相似文献
18.
19.
猪嵴病毒（porcine kobuvirus,PKV）是近年来在健康猪和腹泻猪粪便中新检测到的小核糖核酸病毒科嵴病毒属成员,可能引起猪的腹泻,对养猪业造成重大经济损失。研究发现,PKV广泛分布在猪中,在腹泻和临床健康猪中均已被检测到,阳性率从3.9%~100.0%各不相同。一个典型的PKV病毒粒子直径为30 nm,基因组全长为8 120 bp,包括1个含2 488个氨基酸的开放性阅读框（ORF）;PKV是典型的小RNA病毒科的基因组结构：1个5'非编码区,1个L蛋白,结构蛋白P1（VP0、VP3和VP1）,非结构蛋白P2（2A、2B和2C）和P3（3A、3B、3C和3D）,1个3'非编码区和1个Poly（A）尾巴。PKV的2B编码区存在30个氨基酸的缺失,VP1蛋白是小RNA病毒科变异最频繁的结构蛋白,其含有主要的抗原表位,可促进机体产生中和抗体。检测PKV的方法有反转录聚合酶链式反应（RT-PCR）、TaqMan探针实时荧光定量RT-PCR和逆转录环介导扩增（RT-LAMP）方法。作者对PKV的分类学、流行概况、基因组结构、遗传特性及检测技术等研究现状做一简要概述,以期为进一步研究及了解PKV提供参考。 相似文献
20.
针对编码非结构蛋白的3D基因合成一对引物进行口蹄疫病毒RT-PCR扩增,不同血清型病毒的RNA样本均显现一条457bp的目的带,与预期设计的长度相符合。在敏感性试验中,O型、A型和AsiaⅠ型病毒的最小RNA检出量分别为0.8ng、8ng和8ng。根据GenBank发表的口蹄疫病毒VP1和2A基因序列,采用多重RT-PCR鉴别口蹄疫病毒血清型,O型、A型和AsiaⅠ型病毒的特异性扩增片段分别为200bp、340bp和500bp。对9份乳鼠感染病料进行检测,确诊为O血清型口蹄疫病毒感染。 相似文献