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1.
Global warming, altering the physiology and irrigation demand of grapevines, has already been perceived in certain premium viticulture regions across the world. Selection of proper rootstocks for especially new cultivars has vital role for a sustainable viticulture under water-deficit conditions. The grapevine cultivar ‘Michele Palieri’ has been finding a good reception on the global markets. The objective of this study was to determine the response of ‘Michele Palieri’ cultivar to deficit irrigation using different rootstocks with distinct genetic origins. Two irrigation regimes (Full Irrigation [FI] and Deficit Irrigation [DI]) were applied to the vines of different grafting combinations of ‘Michele Palieri’ with Kober 5 BB, Richter 99, Richter 110, 140 Ruggeri, 44–53 Malégue or grown on own roots. Two years old vines were cultivated in 60?L pots containing sterile peat under controlled glasshouse conditions. Irrigations were regulated according to soil water matric potential (Ψm) levels using tensiometers. The volume of the irrigation water that has to be applied to attain 100% field capacity was performed as FI, while 50% of FI was considered as DI. The water was transported directly into the pots by micro-irrigation systems consisting of individual spaghetti tubes. The vines of DI treatment showed visible symptoms of mild water stress (e.?g., loss of turgor in shoot tips), but no defoliation or leaf necrosis occurred. DI treatment reduced the gs of ‘Michele Palieri’ scion cultivar in varying levels depending on the rootstock. DI treatment also affected vegetative growth of the scion cultivar in different levels. For example, the greatest decrease (26.7%) in shoot length resulting from DI was determined in ‘Michele Palieri’/5 BB grapevines, followed by own rooted vines (13.1%), while the lowest change (2.4%) was found in vines grafted on 110?R. Overall findings of this study imply that the rootstocks originating from V. berlandiery?×?V. rupestris hybrids (110?R, 99?R, 140?Ru and 44–53?M) better performed in a similar genetic aptitude under deficit irrigation regime while the rootstock 5 BB (V. berlandiery?×?V. rupestris) showed more susceptible responses. On the other hand, the general response of own rooted vines were better than those grafted on 5 BB. Therefore, the use of one of V. berlandiery?×?V. rupestris hybrids may be a better choice for viticulture under semiarid regions.  相似文献   

2.
Plum and apple trees are the most widely spread fruit trees in south-eastern Europe. The main purpose of this paper is to characterize the spatial distribution of roots in medium and course textured soils to improve orchard management. ’Stanley’ plum cultivar grafted on Saint Julien A semi-dwarf rootstock and ’Topaz’ apple cultivar grafted on M.9 dwarf rootstock were studied in a temperate climate and medium and course textured soils. The trench technique was used. There was an intense concentration of roots near the trees and the roots did not occupy the whole soil space between tree rows. The finest tree roots in both species were prevalent. Root density was higher in tree rows versus inter-rows. Both the inter-row and in-row distances can be reduced to increase tree density if light penetration into canopy is sufficient. When a full irrigation regime is applied, a soil depth of 0.8?m would be sufficient for water application, and soil depths of 0.4 to 0.6?m would be recommended for deficit irrigation; water and fertilizers should only be applied over the surface area covering most of the roots. When a more strict control on root activity is desired, the tree roots could be cut vertically at 0.5–0.8?m distance from tree rows. The results could also be used in regions and countries with similar soil texture and climate conditions.  相似文献   

3.
Early ripening sweet cherry cvs ‘Burlat’, ‘Earlise’, ‘Samba’, ‘Souvenir des Charmes’ and clone M, which are all characterised by large fruit size and dark red fruit, on GiSelA 5 rootstock were forced – by closing a polytunnel from 24 March to 20 April 2006 – at Klein-Altendorf research station of Bonn University. A portable gas heater was used for automated frost protection in April. An adjacent uncovered planting at the same spacing of 3.8?m?×?1.75?m served as control. Forcing resulted in a 16–18 days earlier flowering in April relative to the 12–16 days earlier harvest at the beginning of June, resulting in a 2–4 days longer fruit development and retarded ripening. PAR was reduced in the polytunnel by 40% on sunny and 30% on overcast days with a concurrent drop in the humidity to 30% on sunny and 40% on overcast days and nearly 100% relative humidity at night. Polytunnel air temperatures on sunny days were increased – during ventilation – by up to 6?°C relative to the outside, e.g. 33?°C versus 27?°C outside. Forced cherries saved one spray application against aphids compared to those outside. Fully-grown cherry leaves contained 40–60?mg chlorophyll/g FM sufficient for photosynthesis with commensurate chlorophyll contents when grown in the polytunnel compared with those outside, and were deficient in calcium (0.5% DM) and magnesium (0.2% DM), but with a surplus in both nitrogen (3.5% DM) and phosphorus (0.42% DM). The surplus leaf nitrogen was due to excessive nitrogen mineralization in the rich soil under the higher temperatures in the polytunnel which caused the vigorous vegetative growth despite the dwarfing rootstock. Great fluctuations in fertilisation – despite strong flowering and sufficient pollination with honey bees – of between 4–71% may be due to the combination of S-alleles of the cherry varieties, infrequent water supply, nutrient imbalance and microclimate. Trees of cv. ‘Burlat’, ‘Earlise’ and clone M in their fourth leaf yielded an average of 2.5–7.5?kg fruit with 15?kg/tree in one case. Fruit of forced cvs. ‘Burlat’, ‘Souvenir’ and ‘Samba’ were larger compared with those of cv. ‘Earlise’ and clone M, which were smaller as a result of increased fruit load on the tree than those from uncovered control trees. For all five cvs tested, except forced cv. ‘Earlise’, a fruit mass of between 10?g (28?mm ) and 12?g (30?mm) and a sugar content of 12–17% with sugar:acid ratios of 20–37:1 made their fruit suitable for marketing as superior or premium fruit at higher farm-gate prices of 4.5–8?€/kg. Fruits were sufficiently dark red or black and firm for short distance transport and rapid sale. An economic evaluation showed financial gains, when yields exceed 8?kg fruit per tree per year at a farm-gate price of 4.50?€/kg.  相似文献   

4.
In a soil not previously planted with vines, ‘Muscat Gordo Blanco’ (= ‘Gordo’) grafted on to 8 rootstocks did not significantly increase the 7-year cumulative yield compared to own-rooted (control) vines despite the increased vigour (pruning-weight) given by some rootstocks. ‘Gordo’ on 1613 rootstock had the maximum cumulative yield, but this was only 8% greater than control. Growth of vines on rootstocks 62–66 was the most vigorous as judged by trunk circumference.During the course of the trial a number of vines on ‘Salt Creek’ (‘Ramsey’) died and others declined markedly due to graft-incompatibility. Surviving vines yielded 40% less than control due to fewer and smaller bunches. They also had lower Brix and pruning-weight and smaller trunk circumference.Symptoms of Legno riccio virus were observed below the bud union on ‘du Lot’ and 420 A rootstocks, and yield of vines on ‘du Lot’ was lower than control. Due to their poor performance, grafting ‘Gordo’ on ‘Ramsey’ and ‘du Lot’ rootstocks is not recommended. In soil not previously planted with vines, ‘Gordo’ clones on their own roots are the most economical and best to use.  相似文献   

5.
Summary

Six wild species of Eriobotrya were evaluated as rootstocks for domesticated loquat. Twelve-month-old seedlings of Oak leaf loquat (an Oak Leaf loquat ‘Zaozhong No. 6’ hybrid) and wild Fragrant loquat (E. fragrans Champ) had higher root-to-shoot fresh weight (FW) ratios than other wild species. When the domesticated loquat, ‘Zaozhong No. 6’, was grafted onto seedlings of six different wild loquat species, differences in compatibility were observed. Graft compatibility was lower for all combinations compared to ‘Zaozhong No. 6’ grafted onto domesticated loquat seedlings. When wild Henry loquat was used as a rootstock, delayed incompatibility was observed. Differences in soluble sugar contents (SSC) between scion and rootstock were observed, and there was a significant negative correlation between graft compatibility and the difference in SSC values. The influence of the rootstock on anatomical changes at the graft union were observed using a scanning electron microscope (SEM). Wild rootstocks also influenced scion growth, fruit bearing, and fruit quality. Graft combinations which used wild Fragrant loquat as the rootstock had increased cold tolerance and improved scion growth. Wild Fragrant loquat therefore appears to have value as a rootstock for domesticated loquat.  相似文献   

6.
不同苗龄接穗西瓜嫁接体愈合的组织细胞学研究   总被引:1,自引:0,他引:1  
 对不同苗龄接穗的西瓜(葫芦砧木)嫁接苗的嫁接体愈合组织进行组织学和细胞学观察。结果表明:接穗苗龄5 d、7 d和10 d的嫁接苗,嫁接愈合快,嫁接6 d后管状分子已开始分化,8 d后隔离层基本消失,维管束桥形成,近愈合面细胞内有丰富细胞器,嫁接苗的成活率达90%以上;而接穗苗龄为13 d和18 d的嫁接组合在嫁接8 d后愈合面隔离层仍存在,愈合面两侧细胞中细胞器少,鲜见维管束桥连通,嫁接苗的成活率分别为83.3%和72%。因此认为,维管束桥的形成及愈合面细胞中丰富的细胞器,是西瓜嫁接苗成活的组织学和细胞学特征,接穗苗龄小于10 d适合于进行嫁接,嫁接苗成活率高。  相似文献   

7.
影响苹果试管嫁接苗培育的因素   总被引:1,自引:0,他引:1  
用苹果试管苗M26作砧木,‘Gala’作接穗,成功地获得试管嫁接苗。嫁接苗在MS液体纸桥培养基上的生长量与嫁接成活率显著高于MS固体培养基。但是,液体培养基导致砧木基部膨大,形成玻璃苗。随BAP浓度升高,嫁接部位愈伤组织形成快、成活率高,接穗生长量明显增加。过高浓度的BAP(≥0.8mg·1~(-1)),诱导砧木侧枝大量形成、砧木基部膨大、产生玻璃苗,并且显著降低砧木的生根力。试管嫁接苗建立阶段最适的BAP浓度为0.4mg·1~(-1)。试管嫁接苗的生根力随IBA浓度升高而显著提高,在1.0mg·1~(-1)浓度时达最适水平。IBA浓度对试管嫁接苗接穗生长的影响与生根力是一致的。  相似文献   

8.
In composite (scion–rootstock) dwarfing fruit trees, an overgrowth at the graft union is often observed, the severity of which is correlated with degree of dwarfing. The graft union of dwarfing sweet cherry (Prunus avium L.) rootstocks may limit soluble sugar transport or starch mobilization, leading to localized accumulation. Soluble sugars and starch were measured in the tissues surrounding the graft union of young ‘Rainier’ (2002) and ‘Lapins’ (2003) sweet cherry trees on ‘Gisela 5’ (‘Gi 5’; dwarfing) and ‘Colt’ (vigorous) rootstocks. Separate rootstock shank, rootstock, graft union, and scion tissues were analyzed for both starch and soluble sugar content throughout the growing season in both years. Starch concentrations did not vary among locations within the graft union for ‘Rainier’ on either the dwarfing or vigorous rootstock, or for ‘Lapins’ on the dwarfing stock. However, for ‘Lapins’ on ‘Colt’, starch was highest in the rootstock shank and declined vertically (rootstock shank ? rootstock > union > scion). Soluble sugar concentrations were generally similar to or higher in scions on ‘Gi 5’ than on ‘Colt’, and were similar to or lower in the rootstock and rootstock shank on ‘Gi 5’ than on ‘Colt’. Results suggest that rootstock has a significant effect of localized accumulations of carbohydrates above and within the graft union of ‘Gi 5’ and below the graft union of ‘Colt’.  相似文献   

9.
Çoban  Nermin  Öztürk  Ahmet 《Erwerbs-Obstbau》2022,64(2):229-235

In modern pear cultivation, clonal quince and pear rootstocks are preferred because they are easy to maintain and harvest. Also, they form dwarf plants and improve fruit quality compared to pear seedling rootstocks. However, graft incompatibility can be involved between different species or genera. The aim of the study was to determine the graft compatibility of the ‘Deveci’ and ‘Williams’ pear cultivars with different pear and quince rootstocks by carbohydrate analysis. Carbohydrate accumulation in the graft union was also observed with iodized potassium iodide (KI) staining. In terms of rootstocks, there were no differences in starch and carbohydrate content, but statistically differences were found in sugar contents. Significant differences were also found between cultivars and graft union in terms of the examined traits. Sugar content was highest in OHxF 333 and lowest in seedling rootstocks. There were no statistical differences in the starch content between the graft unions of the ‘Deveci’ cultivar, while starch accumulation was higher above the graft union than below and graft union in the ‘Williams’ cultivar grafted on the quince rootstock. In the study, it was determined that there were higher carbohydrate accumulation in the scion and graft union than below the graft union in ‘Williams’ grafted quince rootstocks, which was also confirmed by staining with KI. As a result of the study, it was concluded that carbohydrate accumulation analysis can be used to determine the graft compatibility of the pear cultivars with the different quince and pear rootstocks.

  相似文献   

10.
In autumn 2008, in the research orchard of the University of Natural Resources and Life Sciences in Vienna, the apple cultivar ‘Topaz’ was planted on the rootstocks M9 (clone T337) with and without ‘Rubinola’ as interstem, M26, M7 grafted at 25?cm and at 55?cm respectively, M111 and ‘Bittenfelder seedling’, trained as spindles and tested for eight years according to organic production rules. The trees on seeding grew very vigorously, followed by MM111 and M7 normally grafted; the M7 highly grafted trees showed less vigor, comparable with M26, while the trees on M9 with and without interstem grew very weakly. Tree losses were only observed on M9 without interstem. The highest yields per hectare were found on M7 normally grafted, followed by M26, M7 highly grafted and M9 without interstem. M7 highly grafted showed the highest single fruit weights, followed by MM111, at the other end were M9 with and without interstem, and M26. The rootstock M7 grafted at 55?cm combines a medium growth with high yield and good fruit quality and therefore can be recommended for dry areas like our site. M9 without interstem showed a higher specific yield and fruit firmness after storage compared to the variant with interstem. Whether these advantages for ‘Topaz’ on M9 can be combined with a higher grafting height as effective preventive method to control Phytophthora cactorum, has to be examined in further research.  相似文献   

11.
矮砧及乔砧苹果树嫁接口的解剖观察   总被引:13,自引:0,他引:13  
张新忠  章德明 《园艺学报》1995,22(2):117-122
苹果栽培品种秋富1与M7、M26、八棱海棠3种砧木“T”形芽接1、2年生苗的嫁接口部位徒手切片显微镜观察,结果表明,砧木、接芽产生的大量愈伤组织,有不同方向、不同程度的分化,沿砧木和接芽的原有形成层逐渐分化产生新的形成层,使砧术与接穗维管组织连通;接口附近及过渡区域内各种组织均表现异常,维管分子及纤维细胞短、粗、弯曲,上下排列不同轴,射线细胞所占比例大,伸长较短,直径大;矮化砧M26的嫁接口内愈伤组织细胞层数最多,导管不同轴程度最高;乔化砧八棱海棠的嫁接口内愈伤组织细胞层数最少,导管不同轴程度较轻;半矮化砧M7介于二者之间。  相似文献   

12.
果树砧穗互作研究进展   总被引:4,自引:0,他引:4  
果树砧穗互作相关机理的研究对于揭示嫁接亲和性、营养平衡以及信号调控器官形态建成具有重要意义。综述了果树砧穗互作相关机制,并指出目前存在问题和未来发展方向。研究表明:(1)砧穗间细胞正确识别和信息交流是嫁接正常愈合的关键,生理生化代谢物质也影响嫁接体的愈合;(2)嫁接体成活后,砧木直接影响接穗营养水平,接穗调控砧木根系生长发育并产生反馈作用,砧穗间内源激素含量及比例和一些可远距离传递的信号物质参与调控嫁接体发育过程;(3)嫁接杂交为果树育种提供新的方向。未来应进一步研究内源激素如何调控嫁接愈合及嫁接体营养运输、远距离移动的信号物质和嫁接遗传物质改变的分子机制。  相似文献   

13.
 以基砧为八棱海棠的4 种不同中间砧嫁接的苹果幼苗长富2 号/八棱海棠、长富2 号/M9、长 富2 号/M26、长富2 号/SH6 为试验材料,研究干旱胁迫对其导水特性的影响。结果表明:在干旱胁迫下, 4 种中间砧木嫁接苗的整体、冠层、茎干、根系叶比导水率均有减小,各器官叶比导水率基本趋势是乔 化 > 半矮化 > 矮化,其中矮化中间砧的变化幅度最大,乔化中间砧的变化最小。中间砧嫁接口导水阻 力表现为矮化砧比半矮化、乔化砧高,在正常水分条件下,八棱海棠、M9、M26 和SH6 中间砧嫁接区域 导水阻力在植株总体导水阻力中所占的比率分别为4.07%、6.60%、4.97%和5.11%,当受到干旱胁迫后, 嫁接区域所占比率均有不同程度减小。由于矮化苗有效导水率长期低下,根系吸水和运输水分的能力下 降,导致地上部分水分供给减少,从而影响树体的生长。  相似文献   

14.
An objective of this study was to investigate rooting success of grape cuttings propagated from vines symptomatic of Pierce’s disease. Additional objectives were to assess if rooted cuttings could survive and produce viable plants, and determine if Xylella fastidiosa (causal agent of Pierce’s disease) could be found in rooted cuttings. In Jan. 2008, cuttings were taken from symptomatic and asymptomatic ‘Merlot’ and ‘Cabernet Sauvignon’ grapevines growing in the Hill Country and Gulf Coast regions of Texas. Six weeks after cuttings were propagated, each cutting was uprooted and evaluated for rooting and infection parameters. Cuttings were then planted in containers and held in the greenhouse to evaluate survivability. To confirm the presence of X. fastidiosa, propagated cuttings were tested by real-time polymerase chain reaction. Data indicate several rooted cuttings tested positive for X. fastidiosa and appeared viable and healthy. Therefore, vines infected with X. fastidiosa have the ability to produce asexually propagated cuttings, and potentially contaminate non-infected vineyards.  相似文献   

15.
Summary

The feasibility and anatomical development of an in vitro olive cleft-graft method were studied. Grafting survival after 60.d in vitro was 85% which then dropped slightly to 67% after hardening. Three days after grafting, callus formation was observed along the cut surfaces of the rootstock and scion, after 6.d the first healing cellular unions were observed, and by 12.d after grafting a strong union developed. The first cellular differentiation to form vascular tissues was observed 12.d after grafting and continued rapidly until a total connection was reached 10.d later. Thus a complete graft union of the in vitro olive cleft-grafts was achieved in three weeks, and vigorous plants were established after 60.d of in vitro culture followed by 10.d of hardening. The in vitro olive cleft graft is suggested as an effective and useful method for germplasm multiplication.  相似文献   

16.
This study aimed to evaluate in vitro grafting of Theobroma cacao where seedlings of the UF 677 genotype were used as the rootstock and apices or axillary buds of a Trinitarian genotype were used as scion. Three methods of grafting using scions from seedlings were evaluated. Apical grafts using apex and side grafts using apex displayed better graft success (95 and 80%, respectively). However, side grafts using axillary buds reached a greater height on average and a higher number of leaves per plant (1.76 cm and 3.72, respectively). Histological studies revealed new vascular elements at the graft union area. Side grafts with axillary buds provided the highest survival rate (82%) after the acclimatization step. A shoot of at least 1 cm with two leaves is required for plant survival after transfer to ex vitro conditions. Side grafting was carried out with axillary buds from adult trees and nursery plants. Only the grafts with buds from nursery grafted plants were successful, with a rate of 26%. Overall, side grafting with axillary buds is the most appropriate method for cacao micrografting. This method can be used for clonal propagation and for the establishment of in vivo and/or in vitro cacao germplasm collection.  相似文献   

17.
18.
A method was developed for quick propagation of roses under conditions prevailing in The Netherlands, based on cutting and grafting in one action and called “stenting” (to stent), being a contraction of the dutch words “stekken” (to strike a cutting) and “enten” (to graft). A piece of stem of the cultivar with one leaf and a dormant bud is grafted on a single internode of the rootstock. Formation of the graft union and of adventitious roots occur simultaneously, resulting in a complete plant in 3 weeks.Absence of buds on the rootstock is essential to diminish wild suckering. Factors influencing the percentage of successful stentlings were investigated. With R. chinensis ‘Indica Major’ as a rootstock, the best results were obtained with mature internodes 6–8 mm in diameter after application of 5000 mg 1?1 IBA. Pre-treatment of the rootstock internodes by application of IBA and storage at 4° C for 3 weeks improved rooting. Comparison of grafting by hand and by machine showed that machine-grafting offers good possibilities.The influence of the cultivar, after cutting or stenting, on root-formation and root-growth was studied. Grafting on seedling rootstocks (bench-grafting) and stenting proved to give similar results. In the first year after stenting, R. chinensis ‘Indica Major’ was a better rootstock for the cultivar ‘Cocktail 80’ than R. canina ‘Inermis’, while ‘Inermis’ was better for ‘Motrea’.Rose culture can be improved by using cultivars stented on a convenient rootstock. Stenting can be applied around the year, is ideal for screening new scion/stock combinations and to investigate the interaction between shoots and roots.  相似文献   

19.
Summary

‘Hayward’ kiwifruit (Actinidia deliciosa (A. Chev) C. F. Liang et A. R. Ferguson) were sampled to identify populations of fruit with differing fruit mineral concentrations and levels of the storage-related disorder physiological pitting. Fruit were taken from different shoot types at different locations within vines. Fruit from short shoots near the tips of canes, in an area of the vine with low leaf:fruit ratios, had low fruit calcium concentrations and more pitting. In contrast, fruit from long shoots and with high leaf:fruit ratios near the base of canes, had high concentrations of calcium and less pitting. At the more distal positions along canes in an area of the vine with low leaf:fruit ratios, increasing leaf:fruit ratios on individual fruiting shoots led to higher inflow of calcium to the fruit. No such relationship was found when fruit were sampled from shoots near the base of canes, an area of the vine with relatively high leaf:fruit ratios. Inconsistent relationships were found between fruit soluble solids concentrations and calcium, probably due to different mobility of calcium and carbohydrates within the vines. Fruiting position and the associated leaf area are a source of variability in mineral concentrations of fruit, and by consequence, in the incidence of physiological pitting in ‘Hayward’ kiwifruit, and should be considered when developing sampling techniques.  相似文献   

20.
The overall objective of this work was to improve fruit quality, break alternate bearing and reduce hand thinning using fewer chemicals in fruit crops. A device was constructed for mechanical thinning, which consisted of three independent horizontal rotors with ropes and freely adjustable angles on a frame, mounted on a front three point hitch and powered by the tractor hydraulics. This can be adapted to any fruit tree trained as spindle, Solaxe, (tall) vertical axis or fruit wall (le mur fruitier) irrespective of rootstock employed. Rotor speed varied from 300 to 460?rpm at either 5 or 7.5?km/h tractor speed. Eight-year-old or twelve-old apple trees cvs. ‘Gala’ and ‘Golden Delicious’ were mechanically thinned in 2007 between pink bud and full bloom (flower bud stages 6–8 or F1–F2) near Bonn, Germany; non-thinned and hand-thinned apple trees of the same block and variety served as control. Mechanically thinned flowering branches showed a similar amount of ethylene efflux (0.4–0.6?ppm C2H4/branch) as non-thinned flower branches, preventing potentially unexpected subsequent fruit drop, except for those removed by the rotors. The impact of the horizontal rotors on the branches was from the upper side and removed excessive flowers right to the tree trunk viz. the centre of the tree canopy, where fruits of lesser quality are expected leaving 2–3 flowers per cluster. Leaf damage was less than??10%, even at the fast rotor speed of 420?rpm, which was associated with negligible wood injury. Mechanical thinning induced firmer and sweeter fruit, i.e. tastier apples with longer shelf life, relative to control fruit from non-thinned apple trees. The greatest efficacy in terms of final fruit quality in the grading/sorting was achieved by a rotor speed of 360?rpm at a tractor speed of 5?km/h: Fruit mass increased by up to 20?g and the proportion of fruit larger than 70–75?mm by 10–30% compared with the fruit from non-thinned trees. Mechanical thinning with this newly constructed device led to a 10–20% reduction in yield, but increased returns due to better fruit size and colouration in apple with the potential to overcome alternate bearing.  相似文献   

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