共查询到19条相似文献，搜索用时 328 毫秒
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糙皮侧耳多糖分解酶和木质素酶活性研究 总被引：5，自引：0，他引：5
本文测定了侧耳在ＰＤＹ培养液中生长时，培养液ＰＨ值的变化，蛋白质含量和菌丝体研究了淀粉酶，羧甲基纤维素酶，半纤维素酶，邻苯二酚氧化酶，愈创木酚氧化酶和漆酶的活性变化。结果表明；在整个培养欺内六种酶均有酶活性，但不同酶的活性有较大差异，产酶高峰也不尽相同。 相似文献
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侧耳液体培养特性及胞外酶活性研究 总被引：3，自引：2，他引：1
本文测定了侧耳在PDY液体培养基中生长时 ,培养液pH值的变化 ,蛋白质含量和菌丝体重量 ;研究了淀粉酶、羧甲基纤维素酶、半纤维素酶、邻苯二酚氧化酶、愈创木酚氧化酶和漆酶的活性变化。结果表明 :在整个培养基内六种酶均有酶活性 ,但不同酶的活性有较大差异 ,产酶高峰也不尽相同 相似文献
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七个白色金针菇菌株发酵液中四种胞外酶活性的测定与分析 总被引：16，自引：0，他引：16
本文研究了七个白色金针菇杂交菌株在液体深层发酵过程中的滤纸纤维素酶、漆酶、酸性蛋白酶、多酚氧化酶的活性。结果表明,七个白色金针菇菌株中,６号菌株的漆酶、多酚氧化酶、纤维素酶均高于其它６个菌株,而就蛋白酶的活性而言,６号菌株明显低于对照菌株７号。 相似文献
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侧耳792几种胞外酶活性的测定比较 总被引：4，自引：0，他引：4
侧耳(792)在PDY液体培养基中培养时,淀粉酶、羧甲基纤维素酶、半纤维素酶、邻苯二酚氧化酶、愈创木酚氧化酶和漆酶的酶活有较大变化,产酶高峰期也不同；蛋白质含量和菌丝体重量在第10d达最大值,可作为菌丝体(菌种)收获期。 相似文献
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杏鲍菇液体培养胞外酶活性研究 总被引：1，自引：1，他引：0
采用分光光度法测定了杏鲍菇发酵培养时的胞外酶活性。结果表明:淀粉酶、羧甲基纤维素酶、邻苯二酚氧化酶、愈创木酚氧化酶均有活性且活性变化较大。在整个发酵培养过程中,邻苯二酚氧化酶活性较低,淀粉酶活性高峰出现最早,羧甲基纤维素酶活性随培养时间波动上升,而愈创木酚氧化酶在培养后期活性较大。 相似文献
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以梯度试验方法研究了采绒革盖菌（Ｃｏｒｉｏｌｕｓ ｖｅｒｓｉｃｏｌｏｒ）在含不同量枸杞子（Ｌｙｃｉｕｍ ｃｈｉｎｅｎｓｅ）水煎液的ＰＤＹ液体培养基中生长时,纤维素酶、半纤维素酶、木质素酶（漆酶、邻苯二酚氧化酶、愈创木酚氧化酶）活性的变化。结果表明,培养基中含有４％￣５％的枸杞子水提物能明显促进菌丝体生长,显著增强胞内和胞外酶活性。 相似文献
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香菇液体培养的基本生理研究 总被引：2，自引：2，他引：0
供试的三个菌株７４０２，ｘ１６和大光在相同的有机培养基中，其商体量不同，以７４０２生长最多；其漆酶和多酚氧化酶活性也有差异，以Ｘ１６产生的最高。在有机基础培养基中加花生仁纷或葵籽仁粉可以促进菌体生长量，而加草纸浆却提高了木聚糖酶活性。以加漩甲基纤维素的综合培养基为种液，接种到有草纸浆加花生仁粉的培养基中，可以诱导出较高的纤维素酶活性，但漆酶和多酚氧化酶浩性不高。反之，用玉术粉为种液时，仅出刃了高的漆酶和多酚氧化酶活性，而纤维素酶性却是低的。 相似文献
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AIM: To investigate the effects of phosphatase and tensin homolog deleted on chromosome 10 (PTEN) on the apoptosis, oxidative damage and immune inflammatory factors in myocardial H9c2 cells with anoxia/reoxygenation (A/R). METHODS: The H9c2 cells were used to establish a model of A/R. The H9c2 cells were transfected with PTEN small interfering RNA (siRNA) and negative control. After A/R, the expression of PTEN at mRNA and protein levels was determined by RT-PCR and Western blot, respectively. The cell viability was measured by MTT assay. The apoptosis was analyzed by flow cytometry. Xanthine oxidase method was used to determine superoxide dismutase (SOD) activity. The content of malondialdehyde (MDA) was detected by thiobarbituric acid method. The lactate dehydrogenase (LDH) activity in the supernatant was evaluated by 4-dinitrophenylhydrazine method. The levels of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and IL-6 in culture supernatant were examined by ELISA. The protein levels of cleaved caspase-3, Bax and FasL in the cells were determined by Western blot. RESULTS: After A/R, the expression of PTEN at mRNA and protein levels was significantly increased in the H9c2 cells (P<0.05). The mRNA and protein levels of PTEN were decreased significantly after transfection with PTEN siRNA (P<0.05). The viability of H9c2 cells was decreased after A/R, while the apoptotic rate was increased. The protein levels of cleaved caspase-3, Bax and FasL were increased in the cells. The MDA level was elevated, the activity of SOD was decreased, and the levels of LDH, TNF-α, IL-1β and IL-6 in the culture supernatant were increased (P<0.05). Down-regulation of PTEN partly antagonized the effects of A/R on the viability, apoptotic rate, MDA content, SOD activity, and the levels of LDH, TNF-α, IL-1β and IL-6 in culture supernatant. CONCLUSION: Down-regulation of PTEN attenuates oxidative damage induced by A/R, reduces apoptosis and secretion levels of TNF-α, IL-1β and IL-6 in the H9c2 cells. 相似文献
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通过生物信息学方法对苹果赤霉素氧化酶基因GA2ox、GA3ox和GA20ox的结构、化学性质、染色体分布、进化关系、启动子顺式作用元件和组织特异性表达进行分析,并通过实时荧光定量PCR对苹果花芽诱导过程中的表达水平进行测定。从苹果基因组里鉴定出41个赤霉素氧化酶基因,其中GA2ox类20个,GA3ox类14个,GA20ox类7个,除4号染色体外,其他染色体均有分布;其蛋白质分子量在13.15 ~ 60.17 kD之间,等电点预测值在5.50 ~ 9.81之间;通过聚类分析将这41个赤霉素氧化酶基因分为5个亚家族;基因结构和保守结构域分析发现这41个赤霉素氧化酶基因的外显子数1 ~ 5不等,且保守基序Motif 1、5、6、7、10为大部分基因所共有;根据苹果表达量数据库组织特异性分析发现这3类基因在不同品种、不同组织部位的表达量不同,其中在花和果实中表达量相对较高。通过‘长富2号’花芽诱导过程的转录组数据,挑选出8个基因（MdGA2ox4、MdGA2ox6、MdGA2ox9、MdGA2ox12、MdGA3ox5、MdGA3ox12、MdGA20ox1和MdGA20ox5）进行实时荧光定量PCR分析,结果发现,GA3处理后,花后不同时期GA20ox类氧化酶基因表达量均下调,GA2ox类氧化酶基因表达量均上调,GA3ox类氧化酶基因表达量则出现相对波动的现象。 相似文献
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Zhang Xiao-hui 《园艺学报》2012,28(4):714-717
AIM: To study the possible anti-platelet aggregative mechanisms of procyanidins (PC) isolated from grape seeds in vitro. METHODS: Platelet-rich plasma (PRP) and platelet-poor plasma (PPP) were prepared from the blood of healthy volunteers. PC,diphenylene iodonium(DPI,a nonspecific NADPH oxidase inhibitor) and apocynin (a specific NADPH oxidase inhibitor) were used to observe the effects on collagen-induced platelet maximum aggregation rate using platelet aggregometer. The influences of PC on platelet NADPH oxidase activity, NO content and superoxide anion (O2-·) level were evaluated by chemiluminescence spectrometer. The role of PC in the expression of activated platelet markers (PAC-1 and CD62P) was observed by flow cytometry. RESULTS: PC (100 μmol/L), apocynin (10 μmol/L) and DPI (100 μmol/L) significantly inhibited collagen-induced maximum platelet aggregation rate (P<0.01). In collagen-activated platelets, NO content reduced and O2-· level increased,both of which were recovered by PC at concentration of 100 μmol/L (P<0.05). PC also obviously inhibited NADPH oxidase activity (P<0.01), and significantly down-regulated PAC-1 and CD62P expression (P< 0.05) in platelets. CONCLUSION: Procyanidins isolated from grape seeds have the anti-platelet aggregation function through inhibiting NADPH oxidase activity, further influencing platelet NO and O2-· levels. 相似文献
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AIM: To study the increased level of reactive oxygen species in human umbilical vein endothelial cells (HUVECs) and their correlation with the injury caused by cholesterol on HUVECs, and to clarify the original source of intracellular ROS. METHODS: The cells of HUVECs-12 were cultured in F12 medium with 10% FBS and divided into normal control group (without any treatment), solvent group (treated with 0.25% dehydrated alcohol), cholesterol group (treated with 50 mg/L cholesterol) and N-acetyl-L-cysteine(NAC) group(pretreated with 10 mmol/L NAC for 1 h and then treated with 50 mg/L cholesterol for 48 h). The intracellular ROS levels were determined by flow cytometry (FCM) with DCFH-DA as fluorescent probe. Nuclear translocation of NF-κB subunit p65 was detected by immunocytochemistry staining. LDH activity and concentration of nitric oxide in the supernatant of the cell culture were also determined. The concentration of MCP-1 protein in cultured supernatant was measured by ELISA. The intracellular levels of ROS and the changes after adding 4 kinds of enzyme inhibitors (NADPH oxidase inhibitor diphenyl iodide, mitochondrial respiratory chain enzyme complex inhibitor rotenone, NOS inhibitor L-NAME and xanthine oxidase inhibitor oxypurinol) were observed. RESULTS: (1)Compared to the normal control cells, 50 mg/L cholesterol increased intracellular ROS (P<0.01) and activated the nuclear translocation of NF-κB p65. A significant increases in LDH activity and the MCP-l protein were also observed. The NO level decreased in the cells. (2)Compared to the cholesterol control cells, diphenyl iodide decreased intracellular ROS significantly (P<0.01).Retenone also inhibited the generation of ROS partially (P<0.05). The other inhibitors almost did not affect the level of ROS caused by cholesterol (P>0.05). CONCLUSION: Free cholesterol increases ROS generation in endothelial cells, activates intracellular NF-κB, thus leading to endothelial cell injury. NADPH oxidase was the main source of ROS generation in HUVECs cultured with free cholesterol. 相似文献
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平阴玫瑰组培苗多酚含量及多酚氧化酶活性与其生根的关系 总被引：5，自引：0，他引：5
对平阴玫瑰(Rosa rugosa) 多酚含量及多酚氧化酶( Polyphenol oxidase, PPO) 活性与组培苗生根的关系进行了研究, 对多酚含量、PPO活性与生根指数进行了相关性分析。结果表明: 平阴玫瑰不同品种间的多酚含量、PPO活性及生根指数存在极显著差异。多酚含量与生根指数显著负相关, PPO活性与生根指数极显著负相关。对不同pH条件下‘紫枝玫瑰’组培苗的PPO活性和生根指数的研究表明, ‘紫枝玫瑰’组培苗的PPO活性及生根指数与pH均有密切联系, pH为6.5时PPO活性最高, 而生根指数最低, pH 5.5最适宜生根。 相似文献
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Wang Li-Ping 《园艺学报》2014,30(10):1760-1764
AIM:To explore the effect of erythropoietin （EPO） on the expression of myocardial NADPH oxidase （Nox） in the pressure overload rats. METHODS:Male SD rats (n=36) were used to establish a pressure overload myocardial hypertrophy model by abdominal aorta ligation. The animals were divided into model group, control group (sham, without narrowing abdominal aorta, the rest of the operation was the same as the model) and recombinant human erythropoietin (rhEPO) treatment group (intraperitoneal injection of rhEPO postoperatively, 4 000 U/kg, twice a week). After 8 weeks, the cardiac ultrasound imaging and hemodynamic evaluation were conducted to determine the cardiac functions. Masson staining was used to observe the degree of myocardial fibrosis. The expression of Nox2 and Nox4 at mRNA and protein levels was detected by real-time quantitative PCR and Western blotting. The protein levels of myocardial inflammatory factors CD45, F4/80 and TGF-β were determined by Western blotting. RESULTS:Compared with model group, the left ventricular ejection fraction (LVEF), left ventricular fractional shortening (LVFS), left ventricular end-systolic pressure (LVESP) and left ventricular pressure maximum rising and falling rates (±dp/dtmax) increased significantly in EPO treatment group (P<0.01). At the same time, left ventricular end-systolic diameter (LVESD), left ventricular end-diastolic diameter (LVEDD) and left ventricular end-diastolic pressure (LVEDP) were decreased in EPO treatment group (P<0.01). EPO reduced the degree of myocardial fibrosis caused by pressure overload (P<0.01) and decreased the expression of Nox2 and Nox4 at mRNA and protein levels in the myocardium (P<0.05 or P<0.01), and reduced the protein expression of myocardial inflammatory factors CD45, F4/80 and TGF-β. CONCLUSION: EPO inhibits rat myocar-dial fibrosis induced by pressure overload, improves heart functions by decreasing NADPH oxidase activity and inhibiting myocardial oxidative stress levels and myocardial inflammatory reaction. 相似文献