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对民和县176头奶牛的隐性乳房炎进行检测,发现该地区奶牛隐性乳房炎头患病率为75.5%,对乳区进行检测,发现左前乳房患病率为12.5%,左后乳房患病率为27.8%,右前乳房患病率为14.8%,右后乳房患病率为20.4%;通过挤奶方式和胎次对奶牛隐性乳房炎患病率影响的调查,发现人工挤奶的奶牛隐形乳房炎患病率为69.9%,低于机器挤奶的奶牛隐性乳房炎74.3%患病率,奶牛隐性乳房炎患病率从第五胎起有上升趋势。对奶牛隐性乳房炎的患病原因进行调查分析并制定了预防措施,6个月后复检,奶牛隐性乳房炎患病率降低至60.0%。 相似文献
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新疆石河子地区奶牛隐性乳房炎的调查与分析 总被引:3,自引:0,他引:3
采用LMT法对新疆石河子地区三个奶牛场泌乳牛群进行了隐性乳房炎检测,共检测446头泌乳牛1784个乳区。结果表明:奶牛隐性乳房炎阳性率为89.68%,乳区阳性率为59.81%。数据分析表明,不同年龄、胎次奶牛的隐性乳房炎阳性率差异显著(P<0.05),后乳区的隐性乳房炎感染率显著高于前乳区感染率(P<0.05),隐性乳房炎乳区发生数也明显影响当日产奶量(P<0.05)。 相似文献
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The influence of housing factors on udder diseases was investigated in 2156 tiestall barns with 41893 cows and in 98 loose housed herds with 4126 cows. In loose housed herds less teat injuries were observed and also less mastitis than in tiestall barns. Within the different tiestall systems there was a trend to more udder diseases in short standings with a dung grid than in other systems. The ordinary neck chain and the "Grabner"-tethering system showed better results than the horizontal chain and the yoke tying system. Stall partitions between the cows caused a marked reduction of udder diseases. The installation of a cow trainer showed advantages regarding udder diseases. Inaccurate standing measurements (length and width) caused more udder diseases. When the metal grid was in poor condition, the incidence of teat injuries as well as udder diseases of heifers increased. Teat injuries and udder infections were more frequent when limb or digit injuries were found. Regular hoof care twice a year reduced the udder disease rate. Bedding material influenced the incidence of udder diseases positively. On farms where the cows were on pasture, the incidence of udder infections was higher than on farms where the cows were permanently housed. Heifers that were tied as calves or as juveniles showed significantly less udder diseases than those that were raised in groups. Faults in milking, defects in milking machines and deficits in milking hygiene showed a negative effect on udder diseases. 相似文献
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This report describes a 7-year-old cow of the breed "Deutsche Rotbunte" which was brought into the clinic because of intense fungal mastitis and left displacement of the abomasum. A disseminated intravascular coagulopathy (DIC) was determined by coagulation assays and verified by necropsy and histopathological examination. 相似文献
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2002年1月至2003年10月,对苏北盐城地区几个不同饲养环境的奶牛场进行隐性乳腺炎的SMT(上海乳腺炎检验法)检测,结果显示:不同的乳区、有无运动场、原发病情况、发病是否隔离等条件的差异,隐性乳腺炎的发生率存在明显差异;不同的季节、挤奶方式、饲料的营养状况与隐性乳腺炎的发生率没有明显的关系。 相似文献
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The efficiency of regular post-partum gynaecological care in the dairy cow can only be correctly evaluated if cows with a normal post-partum period and those with problems during this time are distinguished. From 1,125 dairy cows being fed a high proportion of maize silage it was shown that gynaecological examinations on days 12, 28 and 40 post-partum had a positive effect on herd fertility. Regular checks resulted in significant reductions in the calving to conception interval, the insemination index and in the percentage culled due to fertility problems in comparison to the control animals (n = 451) where veterinary control took place only occasionally. In particular the early detection and treatment of endometritis and ovary function abnormalities contributed to a high conception rate with appropriate calving interval. The administration of GnRH (20 micrograms Buserelin) to cows with problems in the post-partum period and under continuous gynaecological supervision resulted in improvements in only some aspects (uterine involution, ovarian cyst incidence). The conception rates in the treated group and in the intensively controlled group were the same. 相似文献
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为分析牙鲆碱性磷酸酶基因5'调控区和第一内含子调控序列的功能,本研究运用DNA重组技术将PCR扩增得到的牙鲆碱性磷酸酶基因的5′调控区序列、第一内含子序列及5′调控区和第一内含子串联序列分别插入启动子缺失的增强型绿色荧光蛋白表达载体pAcGFP1-1中,得到pAcGFP1-5′ALP、pAcGFP1-Intron1和pAcGFP1-AI报告基因表达载体。通过脂质体介导重组质粒转染中国仓鼠卵巢细胞(CHO)并检测荧光信号。转染后细胞状态良好,24 h后发现,在倒置荧光显微镜下均可看到不同强度的绿色荧光信号,且荧光信号随时间的延长而增强,其中转染pAcGFP1-AI后的荧光信号强度最强。以上结果表明,牙鲆碱性磷酸酶基因5′调控区和第一个内含子序列均具有启动子活性,且第一内含子与5′调控区存在协同效应,两者协同能增强基因的表达。为了解甲状腺激素T3对调控序列启动子活性的调节作用,用不同浓度的T3处理转染的CHO细胞,24 h后发现加入50、75和100 nM的T3都增强了绿色荧光蛋白的表达,而且信号强度随T3浓度的增加而增强。这表明甲状腺激素T3对牙鲆碱性磷酸酶基因调控序列的启动子活性有一定的增强作用。本研究为深入阐明牙鲆碱性磷酸酶基因转录表达的分子机制提供了实验依据。 相似文献
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RanGTPases, one superfamily of small G protein family, have been demonstrated to be involved in the innate immune system and they are up‐regulated after pathogen infection. However, the regulation mechanism of RanGTPases remains unclear. In this study, the 5′ flanking region of shrimp RanGTPase gene was cloned and sequenced. To detect the activity of the promoter, pIZΔIE/EGFP was modified from pIZ/V5‐His by replacing the OPIE2 promoter with this promoter and inserting enhanced green fluorescence protein (EGFP) gene downstream. The promoter can drive the expression of reporter EGFP gene in Hi5 insect cells, indicating the promoter has activity. These results extend our previous findings and provide insights into the molecular regulation of RanGTPase gene expression, which will be helpful for shrimp viral disease control. 相似文献
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In the mammary gland of cattle there is a complex defense system of non-specific and specific reactions available preventing the invasion of pathogenic bacteria. Most infections occur via the teat canal, so teat canal keratin (SKK) is of particular importance in non-specific defense of the gland. The SKK serves as a physical barrier, and bacteriostatic and/or bactericidal effects of SKK lipids and proteins against certain mastitis bacteria could be demonstrated. By increasing the concentrations of lactoferrin and lysozyme in milk a reduction of mastitis frequencies could be observed. However, those high concentrations in the proteins occur only during the dry period of the cow. An improvement of the mastitis situation would also appear possible by increasing phagocytosis. The numerous trials intended to reduce mastitis by improving specific protection showed no significant success. Therefore, the most successful and cheapest means to achieve udder health remains the strict and consistent hygiene of housing, animals and mammary glands. 相似文献
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M Vandeplassche 《Tier?rztliche Praxis》1985,13(4):513-517
The diagnosis of Mycoplasma bovis mastitis (MbM) usually comes too late. Consequently the capital early symptoms are described for a probable diagnosis in order to enable prophylactic measures and to prevent ineffective expensive treatments and loss of time. Following a definite diagnosis of MbM by culture, a repeated application of the CMT milk test combined with bacteriological culture detects infected cows and they should be culled. All cows of exceptional breeding value can be treated by repeated intramammary infusions of specific antimycoplasma antibiotics with a fair chance of healing, by preference during the dryudder period. 相似文献
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ghrelin是脊椎动物的一种脑肠肽,有促进摄食的功能,并能促进生长激素(GH)释放,参与能量平衡调控和糖类代谢。为探索ghrelin基因多态性与大口黑鲈生长性状的相关性,针对大口黑鲈ghrelin基因的启动子序列,实验采用直接测序法获得了2个SNPs位点:S1(A-642C)和S2(A-639C)。随机选取同批繁殖、同塘养殖的大口黑鲈采用Sna Pshot方法进行SNPs位点检测和分型。结果显示,实验群体在ghrelin基因2个SNPs位点上基本处于哈温平衡,S1和S2共组成5种双倍型(D1、D2、D3、D4和D5)。基因型与生长性状的相关性分析结果表明,S1位点AC型个体在体高与全长上显著高于CC型个体,S2位点AA型个体在头长上显著高于AC型个体。双倍型D1在体质量、全长和体高方面显著高于双倍型D3,在体质量、体宽和体高方面显著高于双倍型D4。本研究在大口黑鲈ghrelin基因启动子区域获得的与生长性状相关的SNPs标记,可为大口黑鲈分子标记辅助育种提供帮助。 相似文献