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为充分发掘利用水稻田已有的有益微生物资源,获得水稻纹枯病菌(Rhizoctonia solani)的拮抗芽孢杆菌菌株,从四川农业大学水稻试验田中采集根际土样,采用稀释平板法在LB培养基上分离得到96株芽孢杆菌菌株。采用平板对峙法,将水稻纹枯病菌标准菌株R.solani AG1 IA与待测芽孢杆菌菌株共培养,得到1株对R.solani AG1 IA具有强拮抗作用的芽孢杆菌菌株Q9-0-1,并以形态学特征、生理生化特性为基础,结合gyr B基因序列分析,将其鉴定为短小芽孢杆菌(Bacillus pumilus)。 相似文献
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《中国水稻研究通报》2002,(Z1)
Enriched by the medium containing chitin and cell wall of Rhizoctonia solani AG-1, a bacterium X2-23 with higher chitinase activity was isolated from 166 chitinase-producing bacteria. It could distinctly inhibit the fungi growth of some plant pathogens such as R. solani, Magnaporth griesa, Fusariwn moniliforme, F. geamin-eanun, Scleretimia sclerotiorum, and 相似文献
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棉苗立枯病丝核菌Rhizoctonia Solani是引起土传病害的重要病原菌之一,在我国棉区发生普遍,寄主范围甚广。我国对R.solani引起的棉苗立枯病进行 相似文献
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河北省马铃薯真菌病害种类及分布 总被引：2，自引：1，他引：2
2001年7月至2002年12月对河北省马铃薯在生长期和储存期进行病害种类调查,共发现了5种主要真菌病害,即早疫病、晚疫病、枯萎病、干腐病和马铃薯黑痣病,并明确了它们在河北省范围内的大致分布。通过对河北省马铃薯真菌病害的病原进行鉴定,共鉴定出了7种病原菌,即:Fusarium solani、Fusarium semiteatum、Fusarium moniliforme、Fusarium oxysporum、Rhizoctonia solani、Phytophthora infestans、Alternaria solani。 相似文献
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一、室内药效测定 试验 方 法 本试验供试的病菌是立枯病菌(Rhizoctonia solani)、根腐病菌(Fusarium sp.)及茎腐病菌(Diplodia sp.)三种。这些病菌都是从花生种籽上分离的。 试验用的“苯骈咪唑44”粉剂,系沈阳化工研究院在北京双桥化工厂生产的成品,含50％的有效成份。 相似文献
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《中国水稻研究通报》2002,(2)
Rhizoctonia solani AG-1 IA, the non-specific, soil-borne, and plant casual a-gent of rice sheath blight, occurring world-widely in rice fields, has become a major disease to rice. In this study, relationships among R. solani AG-1 IA isolates, collected from different rice cultivars were reported.In 1999, the genetic diversity of 40 isolates of rice sheath blight collected from six rice varieties were analyzed by using random amplified polymorphic 相似文献
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Wenlei Cao Xinxin Cao Jianhua Zhao Zhaoyang Zhang Zhiming Feng Shouqiang Ouyang Shimin Zuo 《水稻科学》2020,27(2):101-112
MicroRNAs(miRNAs) are about 22 nucleotides regulatory non-coding RNAs that play versatile roles in reprogramming plant responses to biotic and abiotic stresses. However, it remains unknown whether miRNAs confer the resistance to necrotrophic fungus Rhizoctonia solani in rice. To investigate whether miRNAs regulate the resistance to R. solani, we constructed 12 small RNA libraries from susceptible and resistant rice cultivars treated with water/pathogen at 5 h post inoculation(hpi), 10 hpi and 20 hpi, respectively. By taking the advantage of next-generation sequencing, we totally collected 400–450 known mi RNAs and 450–620 novel miRNAs from the libraries. Expression analysis of mi RNAs demonstrated different patterns for known and novel miRNAs upon R. solani challenge. Thirty-four mi RNA families were identified to be expressed specifically in rice, and most of them were involved in plant disease resistance. A particular Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway analysis result revealed that a great majority of target genes of regulated miRNAs belonged to the pathway of plant-pathogen interaction. Moreover, miR444 b.2, miR531 a, mir1861 i, novel_miR1956 and novel_miR135 conferred response to R. solani infection confirmed by Northern blot. Our global understanding of miRNA profiling revealed that the regulation of mi RNAs may be implicated in the control of rice immunity to R. solani. Analysis of the expression of miRNAs will offer the community with a direction to generate appropriate strategies for controlling rice sheath blight disease. 相似文献
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枯草芽孢杆菌A30菌株产生的拮抗肽的分离纯化与理化性质研究 总被引：24，自引：0，他引：24
平皿测试表明Bacillus subtilis A30菌株对多种植物病原真菌,例如水稻纹枯病菌(Rhizoctonia solani)、稻瘟病菌（Magnaporthe grisea）等有强烈抑制作用。在小区试验中A30菌液对水稻纹枯病也表现出了较好的防治效果。A30菌株培养液经硫酸铵沉淀、FPLC疏水色谱、HPLC反相色谱后,纯化得到一种拮抗活性物质,命名为P1。P1对热稳定,对蛋白酶不敏感;茚三酮反应呈阴性,用酸水解后茚三酮反应和双缩脲反应呈阳性。经LC-ESI-MS分析初步判断P1的分子量为1476.7 Da。 相似文献
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在越南雨季,3～5龄的榴莲植株上常发生由立枯丝核菌（Rhizoctonia solani）引起的叶枯病,其症状表现为淡褐色的边缘不规则病斑。在潮湿环境下,病斑上长有浅黄色菌丝,被感染的叶片逐渐变成深褐色进而枯萎。过去没有立枯丝核菌融合群（AG）和亚群在榴莲上发生危害的报道。 相似文献
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Rahman Moni Zakiah Ansar Ali Md Shahidul Alam Md Asif Rahman Md Rejwan Bhuiyan Md Salim Mian Md Md Iftekharuddaula Khandakar Abdul Latif Md Ashik Iqbal Khan Mohammad 《水稻科学》2016,23(1):42
Eighteen isolates of Rhizoctonia solani collected from infected rice plants in four different locations of Bangladesh were studied by using morphological characters and molecular markers. Anastomosis study with a reference isolate confirmed that all the isolates belonged to R. solani. Significant variation was observed in sclerotial size, shape and distribution. Un-weighted pair group method with arithmetic mean dendrogram constructed based on the Gower's general similarity coefficient showed that these isolates were grouped into four clusters at the 0.68 similarity coefficent according to morphological characters. Cluster I was a major cluster consisting of 13 isolates, while clusters Ⅱ to Ⅳ consisted of 1 or 2 isolates. Analyses by variable number of tandem repeat and amplified fragment length polymorphism markers showed that the isolates were grouped into five and three clusters at a similarity coefficient of 0.64 and 0.69, respectively. Although most of the variability was found between isolates from different regions as expected, significant variation was observed within the isolates collected from similar agro-ecological regions. Our results suggest the presence of different races of R. solani within the same local geographic regions. 相似文献
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大豆根腐病菌拮抗细菌筛选及抗生作用 总被引：6，自引：1，他引：5
从大豆根际土壤中分离纯化得到388个细菌菌株,从中筛选对大豆根腐病病原尖孢镰刀菌(Fusaritm oxysporum)、茄腐镰刀菌(Fusarium solani)和立枯丝核菌(Rhizoctonia solani)有拮抗作用的菌株,采用无菌室内点接法初筛,抑菌带大于20mm以上的有12株,通过盆栽防效试验,进一步证明12个菌株中有6个菌株盆栽防治尖孢镰刀菌和立枯丝核菌达到46.0%-78.5%.菌株与病原真菌共培养结果显示,菌株B2、B57、B85和B69使尖孢镰刀菌菌丝干重分别减少51.27%,40.78%、38.87%和34.58%;菌株B57使茄腐镰刀菌菌丝干重减少71.48%.菌株分泌物对病原真菌的拮抗作用研究结果表明,B57的分泌物对尖孢镰刀菌抑制率最高,3d为82.1%,6d为90.6%;其次是B16,3d为58.8%,6d为51.1%. 相似文献
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白木香立枯病的病原鉴定及生物学特性 总被引：5，自引：0，他引：5
对白木香幼苗上发生的1种新病害进行了病原鉴定及生物学特性研究.结果表明:该病害为丝核菌属的立枯丝核菌(Rhizoctonia solani Kuhn)引起的立枯病.病原菌生长的最适宜温度为10～30℃,20～30℃;在查彼克琼脂培养基上生长最快,香石竹PDA培养基上产菌核最多;以可溶性淀粉为碳源,硫酸氨为氮源,有利于菌丝的生长:光照条件下比黑暗条件下菌丝生长好,但差异不明显. 相似文献
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测定了立枯丝核菌 Rhizoctonia So-lani 的2,3和4联合组群分离菌诱致亚麻枯萎病的能力。所有分离菌诱致种子腐烂,但只有 AG 4分离菌感染亚麻胚轴。AG2和 AG3分离菌诱致有限的根腐。发现两个亚麻品种能抗全部供试分离菌。 相似文献
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人参(Panax ginseng C.A.Mey.)的根腐病是农田栽培人参较严重的病害,经分离鉴定致病菌至少有以下几种(1)Rhizoctonia solani Knohu.(2)Fusariumsolani.(3)Cephalosporium Spp.(4)ThielariopsisSPP.(5)Ra mulariaSpp.。据调查,一、二年生参苗的发病率约20～30%,六年生死亡率达50%以上,造成人参严重减产。为了寻找土壤消毒的最佳农药,我们于1983～1985年进行了室内农药筛选与田间防治效果的试验,结果总结如下: 相似文献
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已经报导有五种镰刀菌为害花生,即Fusariumoxysporum Schlecht.emend Snyd.＆Hans,F.solani(Mart)App.＆Wr.emend.Snyd.＆Hans,Froseum(Lk.ex Fr)emend Snyd.＆Hans,F.tricinctum(Cda)Sacc.emend Snyd.＆Hans,F.moniliforme Sheld.emend Snxd.＆Hans。关于确定镰刀菌是花生病害初侵染病原的文献很少。使花生根部和茎部发生病害的报导有Miller和Harvey(F。solani f.sp.phaseoli(Burk)Snyd＆Hans),Rothwell(F.oxysporum)和Frezzi 相似文献
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近年来,对品种、杂种与不断变化的周围环境之间关系的评价方法的深入研究,一直吸引着国内外许多研究者(Wricke G., 1962, 1966; Eberhart S. A., Russell W. A., 1966; ?., 1973; ?., 1980, 1984; ? 1984等等). 相似文献
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